LOCUS       sequence-410547-        9023 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Mammalian expression of SREBP-1 (isoform 1).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9023)
  AUTHORS   Yang M, Mariano J, Su R, Smith CE, Das S, Gill C, Andresson T, 
            Loncarek J, Tsai YC, Weissman AM
  TITLE     SARS-CoV-2 papain-like protease plays multiple roles in regulating 
            cellular proteins in the endoplasmic reticulum.
  JOURNAL   J Biol Chem. 2023 Oct 12;299(12):105346. doi: 
            10.1016/j.jbc.2023.105346.
  PUBMED    37838170
REFERENCE   2  (bases 1 to 9023)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Biol 
            Chem."; date: "2023-10-12"; volume: "299(12):105346. doi"; pages: " 
            10.1016/j.jbc.2023.105346"
COMMENT     Sequence Label: pCDNA3- SREBP-1 (A317-318;A341; A1171)
FEATURES             Location/Qualifiers
     source          1..9023
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(132..149)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    138..362
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      408..836
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(495..514)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     705..726
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(845..865)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        850..1179
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      1030..1165
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     1092..1111
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     CDS             1246..2040
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(1300..1319)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     1910..1929
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     polyA_signal    2214..2335
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(2251..2270)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     2305..2324
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(2384..2400)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(2384..2400)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(2397..2419)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    2408..2424
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(2432..2438,2439..2456,2457..2462))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    2477..2498
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(2615..2632)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(2786..3374)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(2866..2885)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(3545..4336,4337..4405))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     4168..4187
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(4406..4510)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(4585..4604)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        4776..5155
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        5156..5359
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     5310..5330
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     5404..5423
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        5404..5422
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     regulatory      5448..5457
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 cgcctgggcg gtgcgaccac tgtcacttcc agcggagaat tctcaggcgc ccccgtgccc
       61 taccccgacc ccctggagcc ccggtgagcg gccgctcgag tctagtgggc ccgtttaaac
      121 ccgctgatca gcctcgactg tgccttctag ttgccagcca tctgttgttt gcccctcccc
      181 cgtgccttcc ttgaccctgg aaggtgccac tcccactgtc ctttcctaat aaaatgagga
      241 aattgcatcg cattgtctga gtaggtgtca ttctattctg gggggtgggg tggggcagga
      301 cagcaagggg gaggattggg aagacaatag caggcatgct ggggatgcgg tgggctctat
      361 ggcttctgag gcggaaagaa ccagctgggg ctctaggggg tatccccacg cgccctgtag
      421 cggcgcatta agcgcggcgg gtgtggtggt tacgcgcagc gtgaccgcta cacttgccag
      481 cgccctagcg cccgctcctt tcgctttctt cccttccttt ctcgccacgt tcgccggctt
      541 tccccgtcaa gctctaaatc gggggctccc tttagggttc cgatttagtg ctttacggca
      601 cctcgacccc aaaaaacttg attagggtga tggttcacgt agtgggccat cgccctgata
      661 gacggttttt cgccctttga cgttggagtc cacgttcttt aatagtggac tcttgttcca
      721 aactggaaca acactcaacc ctatctcggt ctattctttt gatttataag ggattttgcc
      781 gatttcggcc tattggttaa aaaatgagct gatttaacaa aaatttaacg cgaattaatt
      841 ctgtggaatg tgtgtcagtt agggtgtgga aagtccccag gctccccagc aggcagaagt
      901 atgcaaagca tgcatctcaa ttagtcagca accaggtgtg gaaagtcccc aggctcccca
      961 gcaggcagaa gtatgcaaag catgcatctc aattagtcag caaccatagt cccgccccta
     1021 actccgccca tcccgcccct aactccgccc agttccgccc attctccgcc ccatggctga
     1081 ctaatttttt ttatttatgc agaggccgag gccgcctctg cctctgagct attccagaag
     1141 tagtgaggag gcttttttgg aggcctaggc ttttgcaaaa agctcccggg agcttgtata
     1201 tccattttcg gatctgatca agagacagga tgaggatcgt ttcgcatgat tgaacaagat
     1261 ggattgcacg caggttctcc ggccgcttgg gtggagaggc tattcggcta tgactgggca
     1321 caacagacaa tcggctgctc tgatgccgcc gtgttccggc tgtcagcgca ggggcgcccg
     1381 gttctttttg tcaagaccga cctgtccggt gccctgaatg aactgcagga cgaggcagcg
     1441 cggctatcgt ggctggccac gacgggcgtt ccttgcgcag ctgtgctcga cgttgtcact
     1501 gaagcgggaa gggactggct gctattgggc gaagtgccgg ggcaggatct cctgtcatct
     1561 caccttgctc ctgccgagaa agtatccatc atggctgatg caatgcggcg gctgcatacg
     1621 cttgatccgg ctacctgccc attcgaccac caagcgaaac atcgcatcga gcgagcacgt
     1681 actcggatgg aagccggtct tgtcgatcag gatgatctgg acgaagagca tcaggggctc
     1741 gcgccagccg aactgttcgc caggctcaag gcgcgcatgc ccgacggcga ggatctcgtc
     1801 gtgacccatg gcgatgcctg cttgccgaat atcatggtgg aaaatggccg cttttctgga
     1861 ttcatcgact gtggccggct gggtgtggcg gaccgctatc aggacatagc gttggctacc
     1921 cgtgatattg ctgaagagct tggcggcgaa tgggctgacc gcttcctcgt gctttacggt
     1981 atcgccgctc ccgattcgca gcgcatcgcc ttctatcgcc ttcttgacga gttcttctga
     2041 gcgggactct ggggttcgaa atgaccgacc aagcgacgcc caacctgcca tcacgagatt
     2101 tcgattccac cgccgccttc tatgaaaggt tgggcttcgg aatcgttttc cgggacgccg
     2161 gctggatgat cctccagcgc ggggatctca tgctggagtt cttcgcccac cccaacttgt
     2221 ttattgcagc ttataatggt tacaaataaa gcaatagcat cacaaatttc acaaataaag
     2281 catttttttc actgcattct agttgtggtt tgtccaaact catcaatgta tcttatcatg
     2341 tctgtatacc gtcgacctct agctagagct tggcgtaatc atggtcatag ctgtttcctg
     2401 tgtgaaattg ttatccgctc acaattccac acaacatacg agccggaagc ataaagtgta
     2461 aagcctgggg tgcctaatga gtgagctaac tcacattaat tgcgttgcgc tcactgcccg
     2521 ctttccagtc gggaaacctg tcgtgccagc tgcattaatg aatcggccaa cgcgcgggga
     2581 gaggcggttt gcgtattggg cgctcttccg cttcctcgct cactgactcg ctgcgctcgg
     2641 tcgttcggct gcggcgagcg gtatcagctc actcaaaggc ggtaatacgg ttatccacag
     2701 aatcagggga taacgcagga aagaacatgt gagcaaaagg ccagcaaaag gccaggaacc
     2761 gtaaaaaggc cgcgttgctg gcgtttttcc ataggctccg cccccctgac gagcatcaca
     2821 aaaatcgacg ctcaagtcag aggtggcgaa acccgacagg actataaaga taccaggcgt
     2881 ttccccctgg aagctccctc gtgcgctctc ctgttccgac cctgccgctt accggatacc
     2941 tgtccgcctt tctcccttcg ggaagcgtgg cgctttctca tagctcacgc tgtaggtatc
     3001 tcagttcggt gtaggtcgtt cgctccaagc tgggctgtgt gcacgaaccc cccgttcagc
     3061 ccgaccgctg cgccttatcc ggtaactatc gtcttgagtc caacccggta agacacgact
     3121 tatcgccact ggcagcagcc actggtaaca ggattagcag agcgaggtat gtaggcggtg
     3181 ctacagagtt cttgaagtgg tggcctaact acggctacac tagaagaaca gtatttggta
     3241 tctgcgctct gctgaagcca gttaccttcg gaaaaagagt tggtagctct tgatccggca
     3301 aacaaaccac cgctggtagc ggtggttttt ttgtttgcaa gcagcagatt acgcgcagaa
     3361 aaaaaggatc tcaagaagat cctttgatct tttctacggg gtctgacgct cagtggaacg
     3421 aaaactcacg ttaagggatt ttggtcatga gattatcaaa aaggatcttc acctagatcc
     3481 ttttaaatta aaaatgaagt tttaaatcaa tctaaagtat atatgagtaa acttggtctg
     3541 acagttacca atgcttaatc agtgaggcac ctatctcagc gatctgtcta tttcgttcat
     3601 ccatagttgc ctgactcccc gtcgtgtaga taactacgat acgggagggc ttaccatctg
     3661 gccccagtgc tgcaatgata ccgcgagacc cacgctcacc ggctccagat ttatcagcaa
     3721 taaaccagcc agccggaagg gccgagcgca gaagtggtcc tgcaacttta tccgcctcca
     3781 tccagtctat taattgttgc cgggaagcta gagtaagtag ttcgccagtt aatagtttgc
     3841 gcaacgttgt tgccattgct acaggcatcg tggtgtcacg ctcgtcgttt ggtatggctt
     3901 cattcagctc cggttcccaa cgatcaaggc gagttacatg atcccccatg ttgtgcaaaa
     3961 aagcggttag ctccttcggt cctccgatcg ttgtcagaag taagttggcc gcagtgttat
     4021 cactcatggt tatggcagca ctgcataatt ctcttactgt catgccatcc gtaagatgct
     4081 tttctgtgac tggtgagtac tcaaccaagt cattctgaga atagtgtatg cggcgaccga
     4141 gttgctcttg cccggcgtca atacgggata ataccgcgcc acatagcaga actttaaaag
     4201 tgctcatcat tggaaaacgt tcttcggggc gaaaactctc aaggatctta ccgctgttga
     4261 gatccagttc gatgtaaccc actcgtgcac ccaactgatc ttcagcatct tttactttca
     4321 ccagcgtttc tgggtgagca aaaacaggaa ggcaaaatgc cgcaaaaaag ggaataaggg
     4381 cgacacggaa atgttgaata ctcatactct tcctttttca atattattga agcatttatc
     4441 agggttattg tctcatgagc ggatacatat ttgaatgtat ttagaaaaat aaacaaatag
     4501 gggttccgcg cacatttccc cgaaaagtgc cacctgacgt cgacggatcg ggagatctcc
     4561 cgatccccta tggtgcactc tcagtacaat ctgctctgat gccgcatagt taagccagta
     4621 tctgctccct gcttgtgtgt tggaggtcgc tgagtagtgc gcgagcaaaa tttaagctac
     4681 aacaaggcaa ggcttgaccg acaattgcat gaagaatctg cttagggtta ggcgttttgc
     4741 gctgcttcgc gatgtacggg ccagatatac gcgttgacat tgattattga ctagttatta
     4801 atagtaatca attacggggt cattagttca tagcccatat atggagttcc gcgttacata
     4861 acttacggta aatggcccgc ctggctgacc gcccaacgac ccccgcccat tgacgtcaat
     4921 aatgacgtat gttcccatag taacgccaat agggactttc cattgacgtc aatgggtgga
     4981 gtatttacgg taaactgccc acttggcagt acatcaagtg tatcatatgc caagtacgcc
     5041 ccctattgac gtcaatgacg gtaaatggcc cgcctggcat tatgcccagt acatgacctt
     5101 atgggacttt cctacttggc agtacatcta cgtattagtc atcgctatta ccatggtgat
     5161 gcggttttgg cagtacatca atgggcgtgg atagcggttt gactcacggg gatttccaag
     5221 tctccacccc attgacgtca atgggagttt gttttggcac caaaatcaac gggactttcc
     5281 aaaatgtcgt aacaactccg ccccattgac gcaaatgggc ggtaggcgtg tacggtggga
     5341 ggtctatata agcagagctc tctggctaac tagagaaccc actgcttact ggcttatcga
     5401 aattaatacg actcactata gggagaccca agctggctag cggatccgcc accatggcct
     5461 ctatgaccgg cggccagcaa atgggtatgg cctctatgac cggcggccag caaatgggag
     5521 catcgatgga cgagccaccc ttcagcgagg cggctttgga gcaggcgctg ggcgagccgt
     5581 gcgatctgga cgcggcgctg ctgaccgaca tcgaaggtga agtcggcgcg gggaggggta
     5641 gggccaacgg cctggacgcc ccaagggcgg gcgcagatcg cggagctatg gattgcactt
     5701 tcgaagacat gcttcagctt atcaacaacc aagacagtga cttccctggc ctatttgacc
     5761 caccctatgc tgggagtggg gcagggggca cagaccctgc cagccccgat accagctccc
     5821 caggcagctt gtctccacct cctgccacat tgagctcctc tcttgaagcc ttcctgagcg
     5881 ggccgcaggc agcgccctca cccctgtccc ctccccagcc tgcacccact ccattgaaga
     5941 tgtacccgtc catgcccgct ttctcccctg ggcctggtat caaggaagag tcagtgccac
     6001 tgagcatcct gcagaccccc accccacagc ccctgccagg ggccctcctg ccacagagct
     6061 tcccagcccc agccccaccg cagttcagct ccacccctgt gttaggctac cccagccctc
     6121 cgggaggctt ctctacagga agccctcccg ggaacaccca gcagccgctg cctggcctgc
     6181 cactggcttc cccgccaggg gtcccgcccg tctccttgca cacccaggtc cagagtgtgg
     6241 tcccccagca gctactgaca gtcacagctg cccccacggc agcccctgta acgaccactg
     6301 tgacctcgca gatccagcag gtcccggtcc tgctgcagcc ccacttcatc aaggcagact
     6361 cgctgcttct gacagccatg aagacagacg gagccactgt gaaggcggca ggtctcagtc
     6421 ccctggtctc tggcaccact gtgcagacag ggcctttgcc gaccctggtg agtgccgcaa
     6481 ccatcttggc aacagtccca ctggtcgtag atgcggagaa gctgcctatc aaccggctcg
     6541 cagctgccag caaggccccg gcctctgccc agagccgtgg agagaagcgc acagcccaca
     6601 acgccattga gaagcgctac cgctcctcca tcaatgacaa aatcattgag ctcaaggatc
     6661 tggtggtggg cactgaggca aagctgaata aatctgctgt cttgcgcaag gccatcgact
     6721 acattcgctt tctgcaacac agcaaccaga agcttaagca ggagaaccta agtctgcgca
     6781 ctgctgtcca caaaagcaaa tctctgaagg atctggtgtc ggcctgtggc agtggaggga
     6841 acacagacgt gctcatggag ggcgtgaaga ctgaggtgga ggacacactg accccacccc
     6901 cctcggatgc tggctcacct ttccagagca gccccttgtc ccttggcagc aggggcagtg
     6961 gcagcggtgg cagtggcagt gactcggagc ctgacagccc agtctttgag gacagcaagg
     7021 caaagccaga gcagcggccg tctctgcaca gccgcggcat gctggaccgc tcccgcctgg
     7081 ccctgtgcac gctcgtcttc ctctgcctgt cctgcaaccc cttggcctcc ttgctggggg
     7141 cccgggggct tcccagcccc tcagatacca ccagcgtcta ccatagccct gggcgcaacg
     7201 tgctgggcac cgagtctaga gatggccctg gctgggccca gtggctgctg cccccagtgg
     7261 tctggctgct caatgggctg ttggtgctcg tctccttggt gcttctcttt gtctacggtg
     7321 agccagtcac acggccccac tcaggccccg ccgtgtactt ctggaggcat cgcaagcagg
     7381 ctgacctgga cctggcccgg ggagactttg cccaggctgc ccagcagctg tggctggccc
     7441 tgcgggcact gggccggccc ctgcccacct cccacctgga cctggcttgt agcctcctct
     7501 ggaacctcat ccgtcacctg ctgcagcgtc tctgggtggg ccgctggctg gcaggccggg
     7561 cagggggcct gcagcaggac tgtgctctgc gagtggatgc aagcgccagc gcccgagacg
     7621 cagccctggt ctaccataag ctgcaccagc tgcacaccat ggggaagcac acaggcgggc
     7681 acctcactgc caccaacctg gcgctgagtg ccctgaacct ggcagagtgt gcaggggatg
     7741 ccgtgtctgt ggcgacgctg gccgagatct atgtggcggc tgcattgaga gtgaagacca
     7801 gtctcccacg ggccttgcat tttctgacac gcttcttcct gagcagtgcc cgccaggcct
     7861 gcctggcaca gagtggctca gtgcctcctg ccatgcagtg gctctgccac cccgtgggcc
     7921 accgtttctt cgtggatggg gactggtccg tgctcagtac cccatgggag agcctgtaca
     7981 gcttggccgg gaacccagtg gaccccctgg cccaggtgac tcagctattc cgggaacatc
     8041 tcttagagcg agcactgaac tgtgtgaccc agcccaaccc cagccctggg tcagctgatg
     8101 gggacaagga attttcggat gccctcgggt acctgcagct gctgaacagc tgttctgatg
     8161 ctgcgggggc tcctgcctac agcttctcca tcagttccag catggccacc accaccggcg
     8221 tagacccggt ggccaagtgg tgggcctctc tgacagctgt ggtgatccac tggctgcggc
     8281 gggatgagga ggcggctgag cggctgtgcc cgctggtgga gcacctgccc cgggtgctgc
     8341 aggagtctga gagacccctg cccagggcag ctctgcactc cttcaaggct gcccgggccc
     8401 tgctgggctg tgccaaggca gagtctggtc cagccagcct gaccatctgt gagaaggcca
     8461 gtgggtacct gcaggacagc ctggctacca caccagccag cagctccatt gacaaggccg
     8521 tgcagctgtt cctgtgtgac ctgcttcttg tggtgcgcac cagcctgtgg cggcagcagc
     8581 agcccccggc cccggcccca gcagcccagg gcaccagcag caggccccag gcttccgccc
     8641 ttgagctgcg tggcttccaa cgggacctga gcagcctgag gcggctggca cagagcttcc
     8701 ggcccgccat gcggagggtg ttcctacatg aggccacggc ccggctgatg gcgggggcca
     8761 gccccacacg gacacaccag ctcctcgacc gcagtctgag gcggcgggca ggccccggtg
     8821 gcaaaggagg cgcggtggcg gagctggagc cgcggcccac gcggcgggag cacgcggagg
     8881 ccttgctgct ggcctcctgc tacctgcccc ccggcttcct gtcggcgccc gggcagcgcg
     8941 tgggcatgct ggctgaggcg gcgcgcacac tagagaaact tggcgatcgc cggctgctgc
     9001 acgactgtca gcagatgctc atg
//
