LOCUS       sequence-410554-        6903 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  pDefence15:Eluc (defence-induced promoter with green-shifted 
            luciferase expression cassette).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6903)
  TITLE     Schwessinger Lab Plasmids 2023
REFERENCE   2  (bases 1 to 6903)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: pBD002
FEATURES             Location/Qualifiers
     source          1..6903
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     RBS             2137..2145
                     /label=Shine-Dalgarno sequence
                     /note="full consensus sequence for ribosome-binding sites 
                     upstream of start codons in E. coli; complementary to a 
                     region in the 3' end of the 16S rRNA (Chen et al., 1994)"
     terminator      2306..2558
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     misc_feature    2606..2630
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     CDS             complement(2786..3934)
                     /codon_start=1
                     /product="trans-acting replication protein that binds to 
                     and activates oriV"
                     /label=trfA
                     /translation="MNRTFDRKAYRQELIDAGFSAEDAETIASRTVMRAPRETFQSVGS
                     MVQQATAKIERDSVQLAPPALPAPSAAVERSRRLEQEAAGLAKSMTIDTRGTMTTKKRK
                     TAGEDLAKQVSEAKQAALLKHTKQQIKEMQLSLFDIAPWPDTMRAMPNDTARSALFTTR
                     NKKIPREALQNKVIFHVNKDVKITYTGVELRADDDELVWQQVLEYAKRTPIGEPITFTF
                     YELCQDLGWSINGRYYTKAEECLSRLQATAMGFTSDRVGHLESVSLLHRFRVLDRGKKT
                     SRCQVLIDEEIVVLFAGDHYTKFIWEKYRKLSPTARRMFDYFSSHREPYPLKLETFRLM
                     CGSDSTRVKKWREQVGEACEELRGSGLVEHAWVNDDLVHCKR"
     rep_origin      complement(4270..4858)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(4350..4369)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(5029..5820,5821..5889))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     5652..5671
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(5890..5994)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      6081..6712
                     /label=oriV
                     /note="incP origin of replication"
     misc_feature    6804..6828
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
ORIGIN
        1 tctgtgaaga caatgccgaa ttcggatccg gagacattgt acaaccccgg tttacgttac
       61 acagcagcga gtagtcattt tcagttacac gctatgtatg catgttgacc gggtaagaaa
      121 atcgagacgc cttagccgtg ttgttgcacg taccgaccga cgcggttaag ctagcgaaca
      181 cggcccagat gcatttgaat gtttattaca agctagtttt ttttgcggga aaattacaag
      241 ctagctaggt gtatatatgt cactttcaat cacaagacaa tgtactacat gagagctaat
      301 gtgcggcagg tacgtacata ctatgtgaga aagcgaaaca ttgcattcgt gggggcaagc
      361 acagccaaaa accagtagta ctagtcaact gaaaaaaaaa ctaaaactag tggtgcacgc
      421 tcgaaaagta tttgaaaacg cgtatctagg tgttgtgttg cctactcgtg aaggaagctc
      481 gggcgtttct ctccgtgcac aaggctatag cactatataa ggtcacgccc ggccctaagt
      541 ccaagcactt gacaacccac aaaataacac agatcagatc atacacaccg ccggctcgtg
      601 gatcacttca ctccatcgct attccattga ctgctgagct tcctgaaagc gagttcgttg
      661 gtaaatggag agagagaaga acgtggtgta cggccccgag cccaagcacc ctctgggcaa
      721 cttcaccgcc ggcgagatgc tgtacaacgc tctgcacaag cactcccaca tcccccaggc
      781 catcctggac gtgatgggca acgagtccct ttcctaccag gagttcttcg acactactgt
      841 gaagctgggc cagagcctcc agaactgtgg ctacaagatg aacgatgtcg tgtcgatctg
      901 tgcagagaac aacaagagat tcttcatccc catcatctcc gcctggtaca tcggcatggt
      961 ggtggcccct gtgaacgagg actatatccc agacgagctg tgtaaagtga ccggcatctc
     1021 caagccgatc ctggtcttta ccactaggaa gatcctgcct aaggttttgg aggttaaaga
     1081 cagaaccaac tacataaaga ggatcatcat actggactct gaagagaacc tgctgggctg
     1141 cgagagcctg cacaacttca tgtccaggta ctccgacaac aacctccaaa cattcaagcc
     1201 tctgcactac gaccctgtgg accaggtagc cgccatcctg tgctcctccg gcacaaccgg
     1261 cctgcctaaa ggcgtgatgc agacccacag gaacatctgt gtgagactca cacacgcatc
     1321 tgaccccaga gtgggtacac aactcatccc cggcgtatcc gtgctggcct acctgccatt
     1381 cttccacgcc ttcggcttca gtatcaacct gggctatttc atggtgggcc tgagagtggt
     1441 gatgctccga aggtttaacc aggaggtgtt cctgaaggcc atccaggact acgaggtgag
     1501 gagcgtgatc aacgttccct ccacaatcct gttcctgtcc aagagccctc tggtggacaa
     1561 gtacgaccta tccaccctgg cggagctgtg ctgtggagcc gctcctctgg cgaaggaggt
     1621 ggccgagatc gccgtgaaga ggctgaacct gccagggata cggtgtggct acggtctaac
     1681 agagtctacc tccgccaaca tccatactct gcacaacgag ttcaagtccg gctccctggg
     1741 caaggtgaca ccttacatgg ccgccaagat catcgacagg aacaccggcg aggccctggg
     1801 tccaaaccag gtgggcgagc tgtgcatctg gggacctatg gtaacaaaag gctatgtgaa
     1861 caacccacag gctactaagg aggccatcga cgacgacggc tggctgcact ctggcgactt
     1921 cggctactac gacgaggacg agtatttcta catcgtggac cggtacaagg agctgatcaa
     1981 atacaagggc tatcaggtcg cccctgtgga gctggaggag atcctccttc agcacccagg
     2041 catcagggac gtggccgtcg tgggtatccc tgacatcgag gccggcgagc tgccagccgg
     2101 cttcgtggtg aagcagcccg gcgcccaact caccgctaag gaggtgtacg acttcctggc
     2161 ccagagggtg tctcactcca agtacctgag gggcggcgta aggttcgtgg actctatccc
     2221 caggaacgtg acaggcaaga ttagtcgaaa agagctgagg gaggccctga tggagaaggc
     2281 ttctaagctg taagcttgtc aagcagatcg ttcaaacatt tggcaataaa gtttcttaag
     2341 attgaatcct gttgccggtc ttgcgatgat tatcatataa tttctgttga attacgttaa
     2401 gcatgtaata attaacatgt aatgcatgac gttatttatg agatgggttt ttatgattag
     2461 agtcccgcaa ttatacattt aatacgcgat agaaaacaaa atatagcgcg caaactagga
     2521 taaattatcg cgcgcggtgt catctatgtt actagatcga cgctgcaatt gtcttctgca
     2581 cgaagtggtt taaactatca gtgtttgaca ggatatattg gcgggtaaac ctaagagaaa
     2641 agagcgttta ttagaataat cggatattta aaagggcgtg aaaaggttta tccgttcgtc
     2701 catttgtatg tgcatgccaa ccacagggtt ccccagatca ggcgctggct gctgaacccc
     2761 cagccggaac tgaccccaca aggccctagc gtttgcaatg caccaggtca tcattgaccc
     2821 aggcgtgttc caccaggccg ctgcctcgca actcttcgca ggcttcgccg acctgctcgc
     2881 gccacttctt cacgcgggtg gaatccgatc cgcacatgag gcggaaggtt tccagcttga
     2941 gcgggtacgg ctcccggtgc gagctgaaat agtcgaacat ccgtcgggcc gtcggcgaca
     3001 gcttgcggta cttctcccat atgaatttcg tgtagtggtc gccagcaaac agcacgacga
     3061 tttcctcgtc gatcaggacc tggcaacggg acgttttctt gccacggtcc aggacgcgga
     3121 agcggtgcag cagcgacacc gattccaggt gcccaacgcg gtcggacgtg aagcccatcg
     3181 ccgtcgcctg taggcgcgac aggcattcct cggccttcgt gtaataccgg ccattgatcg
     3241 accagcccag gtcctggcaa agctcgtaga acgtgaaggt gatcggctcg ccgatagggg
     3301 tgcgcttcgc gtactccaac acctgctgcc acaccagttc gtcatcgtcg gcccgcagct
     3361 cgacgccggt gtaggtgatc ttcacgtcct tgttgacgtg gaaaatgacc ttgttttgca
     3421 gcgcctcgcg cgggattttc ttgttgcgcg tggtgaacag ggcagagcgg gccgtgtcgt
     3481 ttggcatcgc tcgcatcgtg tccggccacg gcgcaatatc gaacaaggaa agctgcattt
     3541 ccttgatctg ctgcttcgtg tgtttcagca acgcggcctg cttggcctcg ctgacctgtt
     3601 ttgccaggtc ctcgccggcg gtttttcgct tcttggtcgt catagttcct cgcgtgtcga
     3661 tggtcatcga cttcgccaaa cctgccgcct cctgttcaag acgacgcgaa cgctccacgg
     3721 cggccgatgg cgcgggcagg gcagggggag ccagttgcac gctgtcgcgc tcgatcttgg
     3781 ccgtagcttg ctggaccatc gagccgacgg actggaaggt ttcgcggggc gcacgcatga
     3841 cggtgcggct tgcgatggtt tcggcatcct cggcggaaaa ccccgcgtcg atcagttctt
     3901 gcctgtatgc cttccggtca aacgtccgat tcattcaccc tccttgcggg attgccccga
     3961 ctcacgccgg ggcaatgtgc ccttattcct gatttgaccc gcctggtgcc ttggtgtcca
     4021 gataatccac cttatcggca atgaagtcgg tcccgtagac cgtctggccg tccttctcgt
     4081 acttggtatt ccgaatcttg ccctgcacga ataccagcga ccccttgccc aaatacttgc
     4141 cgtgggcctc ggcctgagag ccaaaacact tgatgcggaa gaagtcggtg cgctcctgct
     4201 tgtcgccggc atcgttgcgc cacatctagg atctgccagg aaccgtaaaa aggccgcgtt
     4261 gctggcgttt ttccataggc tccgcccccc tgacgagcat cacaaaaatc gacgctcaag
     4321 tcagaggtgg cgaaacccga caggactata aagataccag gcgtttcccc ctggaagctc
     4381 cctcgtgcgc tctcctgttc cgaccctgcc gcttaccgga tacctgtccg cctttctccc
     4441 ttcgggaagc gtggcgcttt ctcatagctc acgctgtagg tatctcagtt cggtgtaggt
     4501 cgttcgctcc aagctgggct gtgtgcacga accccccgtt cagcccgacc gctgcgcctt
     4561 atccggtaac tatcgtcttg agtccaaccc ggtaagacac gacttatcgc cactggcagc
     4621 agccactggt aacaggatta gcagagcgag gtatgtaggc ggtgctacag agttcttgaa
     4681 gtggtggcct aactacggct acactagaag aacagtattt ggtatctgcg ctctgctgaa
     4741 gccagttacc ttcggaaaaa gagttggtag ctcttgatcc ggcaaacaaa ccaccgctgg
     4801 tagcggtggt ttttttgttt gcaagcagca gattacgcgc agaaaaaaag gatctcaaga
     4861 agatcctttg atcttttcta cggggtctga cgctcagtgg aacgaaaact cacgttaagg
     4921 gattttggtc atgagattat caaaaaggat cttcacctag atccttttaa attaaaaatg
     4981 aagttttaaa tcaatctaaa gtatatatga gtaaacttgg tctgacagtt accaatgctt
     5041 aatcagtgag gcacctatct cagcgatctg tctatttcgt tcatccatag ttgcctgact
     5101 ccccgtcgtg tagataacta cgatacggga gggcttacca tctggcccca gtgctgcaat
     5161 gataccgcga gaaccacgct caccggctcc agatttatca gcaataaacc agccagccgg
     5221 aagggccgag cgcagaagtg gtcctgcaac tttatccgcc tccatccagt ctattaattg
     5281 ttgccgggaa gctagagtaa gtagttcgcc agttaatagt ttgcgcaacg ttgttgccat
     5341 tgctacaggc atcgtggtgt cacgctcgtc gtttggtatg gcttcattca gctccggttc
     5401 ccaacgatca aggcgagtta catgatcccc catgttgtgc aaaaaagcgg ttagctcctt
     5461 cggtcctccg atcgttgtca gaagtaagtt ggccgcagtg ttatcactca tggttatggc
     5521 agcactgcat aattctctta ctgtcatgcc atccgtaaga tgcttttctg tgactggtga
     5581 gtactcaacc aagtcattct gagaatagtg tatgcggcga ccgagttgct cttgcccggc
     5641 gtcaatacgg gataataccg cgccacatag cagaacttta aaagtgctca tcattggaaa
     5701 acgttcttcg gggcgaaaac tctcaaggat cttaccgctg ttgagatcca gttcgatgta
     5761 acccactcgt gcacccaact gatcttcagc atcttttact ttcaccagcg tttctgggtg
     5821 agcaaaaaca ggaaggcaaa atgccgcaaa aaagggaata agggcgacac ggaaatgttg
     5881 aatactcata ctcttccttt ttcaatatta ttgaagcatt tatcaaggtt attgtctcat
     5941 gagcggatac atatttgaat gtatttagaa aaataaacaa ataggggttc cgcgcacgaa
     6001 ttggccagcg ctgccatttt tggggtgagg ccgttcgcgg ccgaggggcg cagcccctgg
     6061 ggggatggga ggcccgcgtt agcgggccgg gagggttcga gaaggggggg cacccccctt
     6121 cggcgtgcgc ggtcacgcgc acagggcgca gccctggtta aaaacaaggt ttataaatat
     6181 tggtttaaaa gcaggttaaa agacaggtta gcggtggccg aaaaacgggc ggaaaccctt
     6241 gcaaatgctg gattttctgc ctgtggacag cccctcaaat gtcaataggt gcgcccctca
     6301 tctgtcagca ctctgcccct caagtgtcaa ggatcgcgcc cctcatctgt cagtagtcgc
     6361 gcccctcaag tgtcaatacc gcagggcact tatccccagg cttgtccaca tcatctgtgg
     6421 gaaactcgcg taaaatcagg cgttttcgcc gatttgcgag gctggccagc tccacgtcgc
     6481 cggccgaaat cgagcctgcc cctcatctgt caacgccgcg ccgggtgagt cggcccctca
     6541 agtgtcaacg tccgcccctc atctgtcagt gagggccaag ttttccgcga ggtatccaca
     6601 acgccggcgg ccgcggtgtc tcgcacacgg cttcgacggc gtttctggcg cgtttgcagg
     6661 gccatagacg gccgccagcc cagcggcgag ggcaaccagc ccggtgagcg tcgcaaagga
     6721 gatcctgatc tgactgatgg gctgcctgta tcgagtggtg attttgtgcc gagctgccgg
     6781 tcggggagct gttggctggc tggtggcagg atatattgtg gtgtaaacaa attgacgctt
     6841 agacaactta ataacacatt gcggacgttt ttaatgtact ggggtggatg cagtgggccc
     6901 cac
//
