LOCUS       sequence-410555-        9023 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Mammalian expression of SREBP-1 (isoform 1).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9023)
  AUTHORS   Yang M, Mariano J, Su R, Smith CE, Das S, Gill C, Andresson T, 
            Loncarek J, Tsai YC, Weissman AM
  TITLE     SARS-CoV-2 papain-like protease plays multiple roles in regulating 
            cellular proteins in the endoplasmic reticulum.
  JOURNAL   J Biol Chem. 2023 Oct 12;299(12):105346. doi: 
            10.1016/j.jbc.2023.105346.
  PUBMED    37838170
REFERENCE   2  (bases 1 to 9023)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Biol 
            Chem."; date: "2023-10-12"; volume: "299(12):105346. doi"; pages: " 
            10.1016/j.jbc.2023.105346"
COMMENT     Sequence Label: pCDNA3- SREBP-1
FEATURES             Location/Qualifiers
     source          1..9023
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(44..63)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        235..614
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        615..818
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     769..789
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     863..882
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        863..881
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     regulatory      907..916
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     primer_bind     complement(4614..4631)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    4620..4844
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      4890..5318
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(4977..4996)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     5187..5208
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(5327..5347)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        5332..5661
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      5512..5647
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     5574..5593
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     CDS             5728..6522
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(5782..5801)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     6392..6411
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     polyA_signal    6696..6817
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(6733..6752)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     6787..6806
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(6866..6882)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(6866..6882)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(6879..6901)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    6890..6906
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(6914..6920,6921..6938,6939..6944))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    6959..6980
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(7097..7114)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(7268..7856)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(7348..7367)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(8027..8818,8819..8887))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     8650..8669
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(8888..8992)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 gacggatcgg gagatctccc gatcccctat ggtgcactct cagtacaatc tgctctgatg
       61 ccgcatagtt aagccagtat ctgctccctg cttgtgtgtt ggaggtcgct gagtagtgcg
      121 cgagcaaaat ttaagctaca acaaggcaag gcttgaccga caattgcatg aagaatctgc
      181 ttagggttag gcgttttgcg ctgcttcgcg atgtacgggc cagatatacg cgttgacatt
      241 gattattgac tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata
      301 tggagttccg cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc
      361 cccgcccatt gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc
      421 attgacgtca atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt
      481 atcatatgcc aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt
      541 atgcccagta catgacctta tgggactttc ctacttggca gtacatctac gtattagtca
      601 tcgctattac catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg
      661 actcacgggg atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc
      721 aaaatcaacg ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg
      781 gtaggcgtgt acggtgggag gtctatataa gcagagctct ctggctaact agagaaccca
      841 ctgcttactg gcttatcgaa attaatacga ctcactatag ggagacccaa gctggctagc
      901 ggatccgcca ccatggcctc tatgaccggc ggccagcaaa tgggtatggc ctctatgacc
      961 ggcggccagc aaatgggagc atcgatggac gagccaccct tcagcgaggc ggctttggag
     1021 caggcgctgg gcgagccgtg cgatctggac gcggcgctgc tgaccgacat cgaaggtgaa
     1081 gtcggcgcgg ggaggggtag ggccaacggc ctggacgccc caagggcggg cgcagatcgc
     1141 ggagctatgg attgcacttt cgaagacatg cttcagctta tcaacaacca agacagtgac
     1201 ttccctggcc tatttgaccc accctatgct gggagtgggg cagggggcac agaccctgcc
     1261 agccccgata ccagctcccc aggcagcttg tctccacctc ctgccacatt gagctcctct
     1321 cttgaagcct tcctgagcgg gccgcaggca gcgccctcac ccctgtcccc tccccagcct
     1381 gcacccactc cattgaagat gtacccgtcc atgcccgctt tctcccctgg gcctggtatc
     1441 aaggaagagt cagtgccact gagcatcctg cagaccccca ccccacagcc cctgccaggg
     1501 gccctcctgc cacagagctt cccagcccca gccccaccgc agttcagctc cacccctgtg
     1561 ttaggctacc ccagccctcc gggaggcttc tctacaggaa gccctcccgg gaacacccag
     1621 cagccgctgc ctggcctgcc actggcttcc ccgccagggg tcccgcccgt ctccttgcac
     1681 acccaggtcc agagtgtggt cccccagcag ctactgacag tcacagctgc ccccacggca
     1741 gcccctgtaa cgaccactgt gacctcgcag atccagcagg tcccggtcct gctgcagccc
     1801 cacttcatca aggcagactc gctgcttctg acagccatga agacagacgg agccactgtg
     1861 aaggcggcag gtctcagtcc cctggtctct ggcaccactg tgcagacagg gcctttgccg
     1921 accctggtga gtggcggaac catcttggca acagtcccac tggtcgtaga tgcggagaag
     1981 ctgcctatca accggctcgc agctggcagc aaggccccgg cctctgccca gagccgtgga
     2041 gagaagcgca cagcccacaa cgccattgag aagcgctacc gctcctccat caatgacaaa
     2101 atcattgagc tcaaggatct ggtggtgggc actgaggcaa agctgaataa atctgctgtc
     2161 ttgcgcaagg ccatcgacta cattcgcttt ctgcaacaca gcaaccagaa gcttaagcag
     2221 gagaacctaa gtctgcgcac tgctgtccac aaaagcaaat ctctgaagga tctggtgtcg
     2281 gcctgtggca gtggagggaa cacagacgtg ctcatggagg gcgtgaagac tgaggtggag
     2341 gacacactga ccccaccccc ctcggatgct ggctcacctt tccagagcag ccccttgtcc
     2401 cttggcagca ggggcagtgg cagcggtggc agtggcagtg actcggagcc tgacagccca
     2461 gtctttgagg acagcaaggc aaagccagag cagcggccgt ctctgcacag ccgcggcatg
     2521 ctggaccgct cccgcctggc cctgtgcacg ctcgtcttcc tctgcctgtc ctgcaacccc
     2581 ttggcctcct tgctgggggc ccgggggctt cccagcccct cagataccac cagcgtctac
     2641 catagccctg ggcgcaacgt gctgggcacc gagtctagag atggccctgg ctgggcccag
     2701 tggctgctgc ccccagtggt ctggctgctc aatgggctgt tggtgctcgt ctccttggtg
     2761 cttctctttg tctacggtga gccagtcaca cggccccact caggccccgc cgtgtacttc
     2821 tggaggcatc gcaagcaggc tgacctggac ctggcccggg gagactttgc ccaggctgcc
     2881 cagcagctgt ggctggccct gcgggcactg ggccggcccc tgcccacctc ccacctggac
     2941 ctggcttgta gcctcctctg gaacctcatc cgtcacctgc tgcagcgtct ctgggtgggc
     3001 cgctggctgg caggccgggc agggggcctg cagcaggact gtgctctgcg agtggatgca
     3061 agcgccagcg cccgagacgc agccctggtc taccataagc tgcaccagct gcacaccatg
     3121 gggaagcaca caggcgggca cctcactgcc accaacctgg cgctgagtgc cctgaacctg
     3181 gcagagtgtg caggggatgc cgtgtctgtg gcgacgctgg ccgagatcta tgtggcggct
     3241 gcattgagag tgaagaccag tctcccacgg gccttgcatt ttctgacacg cttcttcctg
     3301 agcagtgccc gccaggcctg cctggcacag agtggctcag tgcctcctgc catgcagtgg
     3361 ctctgccacc ccgtgggcca ccgtttcttc gtggatgggg actggtccgt gctcagtacc
     3421 ccatgggaga gcctgtacag cttggccggg aacccagtgg accccctggc ccaggtgact
     3481 cagctattcc gggaacatct cttagagcga gcactgaact gtgtgaccca gcccaacccc
     3541 agccctgggt cagctgatgg ggacaaggaa ttttcggatg ccctcgggta cctgcagctg
     3601 ctgaacagct gttctgatgc tgcgggggct cctgcctaca gcttctccat cagttccagc
     3661 atggccacca ccaccggcgt agacccggtg gccaagtggt gggcctctct gacagctgtg
     3721 gtgatccact ggctgcggcg ggatgaggag gcggctgagc ggctgtgccc gctggtggag
     3781 cacctgcccc gggtgctgca ggagtctgag agacccctgc ccagggcagc tctgcactcc
     3841 ttcaaggctg cccgggccct gctgggctgt gccaaggcag agtctggtcc agccagcctg
     3901 accatctgtg agaaggccag tgggtacctg caggacagcc tggctaccac accagccagc
     3961 agctccattg acaaggccgt gcagctgttc ctgtgtgacc tgcttcttgt ggtgcgcacc
     4021 agcctgtggc ggcagcagca gcccccggcc ccggccccag cagcccaggg caccagcagc
     4081 aggccccagg cttccgccct tgagctgcgt ggcttccaac gggacctgag cagcctgagg
     4141 cggctggcac agagcttccg gcccgccatg cggagggtgt tcctacatga ggccacggcc
     4201 cggctgatgg cgggggccag ccccacacgg acacaccagc tcctcgaccg cagtctgagg
     4261 cggcgggcag gccccggtgg caaaggaggc gcggtggcgg agctggagcc gcggcccacg
     4321 cggcgggagc acgcggaggc cttgctgctg gcctcctgct acctgccccc cggcttcctg
     4381 tcggcgcccg ggcagcgcgt gggcatgctg gctgaggcgg cgcgcacact agagaaactt
     4441 ggcgatcgcc ggctgctgca cgactgtcag cagatgctca tgcgcctggg cggtgggacc
     4501 actgtcactt ccagcggaga attctcaggc gcccccgtgc cctaccccga ccccctggag
     4561 ccccggtgag cggccgctcg agtctagtgg gcccgtttaa acccgctgat cagcctcgac
     4621 tgtgccttct agttgccagc catctgttgt ttgcccctcc cccgtgcctt ccttgaccct
     4681 ggaaggtgcc actcccactg tcctttccta ataaaatgag gaaattgcat cgcattgtct
     4741 gagtaggtgt cattctattc tggggggtgg ggtggggcag gacagcaagg gggaggattg
     4801 ggaagacaat agcaggcatg ctggggatgc ggtgggctct atggcttctg aggcggaaag
     4861 aaccagctgg ggctctaggg ggtatcccca cgcgccctgt agcggcgcat taagcgcggc
     4921 gggtgtggtg gttacgcgca gcgtgaccgc tacacttgcc agcgccctag cgcccgctcc
     4981 tttcgctttc ttcccttcct ttctcgccac gttcgccggc tttccccgtc aagctctaaa
     5041 tcgggggctc cctttagggt tccgatttag tgctttacgg cacctcgacc ccaaaaaact
     5101 tgattagggt gatggttcac gtagtgggcc atcgccctga tagacggttt ttcgcccttt
     5161 gacgttggag tccacgttct ttaatagtgg actcttgttc caaactggaa caacactcaa
     5221 ccctatctcg gtctattctt ttgatttata agggattttg ccgatttcgg cctattggtt
     5281 aaaaaatgag ctgatttaac aaaaatttaa cgcgaattaa ttctgtggaa tgtgtgtcag
     5341 ttagggtgtg gaaagtcccc aggctcccca gcaggcagaa gtatgcaaag catgcatctc
     5401 aattagtcag caaccaggtg tggaaagtcc ccaggctccc cagcaggcag aagtatgcaa
     5461 agcatgcatc tcaattagtc agcaaccata gtcccgcccc taactccgcc catcccgccc
     5521 ctaactccgc ccagttccgc ccattctccg ccccatggct gactaatttt ttttatttat
     5581 gcagaggccg aggccgcctc tgcctctgag ctattccaga agtagtgagg aggctttttt
     5641 ggaggcctag gcttttgcaa aaagctcccg ggagcttgta tatccatttt cggatctgat
     5701 caagagacag gatgaggatc gtttcgcatg attgaacaag atggattgca cgcaggttct
     5761 ccggccgctt gggtggagag gctattcggc tatgactggg cacaacagac aatcggctgc
     5821 tctgatgccg ccgtgttccg gctgtcagcg caggggcgcc cggttctttt tgtcaagacc
     5881 gacctgtccg gtgccctgaa tgaactgcag gacgaggcag cgcggctatc gtggctggcc
     5941 acgacgggcg ttccttgcgc agctgtgctc gacgttgtca ctgaagcggg aagggactgg
     6001 ctgctattgg gcgaagtgcc ggggcaggat ctcctgtcat ctcaccttgc tcctgccgag
     6061 aaagtatcca tcatggctga tgcaatgcgg cggctgcata cgcttgatcc ggctacctgc
     6121 ccattcgacc accaagcgaa acatcgcatc gagcgagcac gtactcggat ggaagccggt
     6181 cttgtcgatc aggatgatct ggacgaagag catcaggggc tcgcgccagc cgaactgttc
     6241 gccaggctca aggcgcgcat gcccgacggc gaggatctcg tcgtgaccca tggcgatgcc
     6301 tgcttgccga atatcatggt ggaaaatggc cgcttttctg gattcatcga ctgtggccgg
     6361 ctgggtgtgg cggaccgcta tcaggacata gcgttggcta cccgtgatat tgctgaagag
     6421 cttggcggcg aatgggctga ccgcttcctc gtgctttacg gtatcgccgc tcccgattcg
     6481 cagcgcatcg ccttctatcg ccttcttgac gagttcttct gagcgggact ctggggttcg
     6541 aaatgaccga ccaagcgacg cccaacctgc catcacgaga tttcgattcc accgccgcct
     6601 tctatgaaag gttgggcttc ggaatcgttt tccgggacgc cggctggatg atcctccagc
     6661 gcggggatct catgctggag ttcttcgccc accccaactt gtttattgca gcttataatg
     6721 gttacaaata aagcaatagc atcacaaatt tcacaaataa agcatttttt tcactgcatt
     6781 ctagttgtgg tttgtccaaa ctcatcaatg tatcttatca tgtctgtata ccgtcgacct
     6841 ctagctagag cttggcgtaa tcatggtcat agctgtttcc tgtgtgaaat tgttatccgc
     6901 tcacaattcc acacaacata cgagccggaa gcataaagtg taaagcctgg ggtgcctaat
     6961 gagtgagcta actcacatta attgcgttgc gctcactgcc cgctttccag tcgggaaacc
     7021 tgtcgtgcca gctgcattaa tgaatcggcc aacgcgcggg gagaggcggt ttgcgtattg
     7081 ggcgctcttc cgcttcctcg ctcactgact cgctgcgctc ggtcgttcgg ctgcggcgag
     7141 cggtatcagc tcactcaaag gcggtaatac ggttatccac agaatcaggg gataacgcag
     7201 gaaagaacat gtgagcaaaa ggccagcaaa aggccaggaa ccgtaaaaag gccgcgttgc
     7261 tggcgttttt ccataggctc cgcccccctg acgagcatca caaaaatcga cgctcaagtc
     7321 agaggtggcg aaacccgaca ggactataaa gataccaggc gtttccccct ggaagctccc
     7381 tcgtgcgctc tcctgttccg accctgccgc ttaccggata cctgtccgcc tttctccctt
     7441 cgggaagcgt ggcgctttct catagctcac gctgtaggta tctcagttcg gtgtaggtcg
     7501 ttcgctccaa gctgggctgt gtgcacgaac cccccgttca gcccgaccgc tgcgccttat
     7561 ccggtaacta tcgtcttgag tccaacccgg taagacacga cttatcgcca ctggcagcag
     7621 ccactggtaa caggattagc agagcgaggt atgtaggcgg tgctacagag ttcttgaagt
     7681 ggtggcctaa ctacggctac actagaagaa cagtatttgg tatctgcgct ctgctgaagc
     7741 cagttacctt cggaaaaaga gttggtagct cttgatccgg caaacaaacc accgctggta
     7801 gcggtggttt ttttgtttgc aagcagcaga ttacgcgcag aaaaaaagga tctcaagaag
     7861 atcctttgat cttttctacg gggtctgacg ctcagtggaa cgaaaactca cgttaaggga
     7921 ttttggtcat gagattatca aaaaggatct tcacctagat ccttttaaat taaaaatgaa
     7981 gttttaaatc aatctaaagt atatatgagt aaacttggtc tgacagttac caatgcttaa
     8041 tcagtgaggc acctatctca gcgatctgtc tatttcgttc atccatagtt gcctgactcc
     8101 ccgtcgtgta gataactacg atacgggagg gcttaccatc tggccccagt gctgcaatga
     8161 taccgcgaga cccacgctca ccggctccag atttatcagc aataaaccag ccagccggaa
     8221 gggccgagcg cagaagtggt cctgcaactt tatccgcctc catccagtct attaattgtt
     8281 gccgggaagc tagagtaagt agttcgccag ttaatagttt gcgcaacgtt gttgccattg
     8341 ctacaggcat cgtggtgtca cgctcgtcgt ttggtatggc ttcattcagc tccggttccc
     8401 aacgatcaag gcgagttaca tgatccccca tgttgtgcaa aaaagcggtt agctccttcg
     8461 gtcctccgat cgttgtcaga agtaagttgg ccgcagtgtt atcactcatg gttatggcag
     8521 cactgcataa ttctcttact gtcatgccat ccgtaagatg cttttctgtg actggtgagt
     8581 actcaaccaa gtcattctga gaatagtgta tgcggcgacc gagttgctct tgcccggcgt
     8641 caatacggga taataccgcg ccacatagca gaactttaaa agtgctcatc attggaaaac
     8701 gttcttcggg gcgaaaactc tcaaggatct taccgctgtt gagatccagt tcgatgtaac
     8761 ccactcgtgc acccaactga tcttcagcat cttttacttt caccagcgtt tctgggtgag
     8821 caaaaacagg aaggcaaaat gccgcaaaaa agggaataag ggcgacacgg aaatgttgaa
     8881 tactcatact cttccttttt caatattatt gaagcattta tcagggttat tgtctcatga
     8941 gcggatacat atttgaatgt atttagaaaa ataaacaaat aggggttccg cgcacatttc
     9001 cccgaaaagt gccacctgac gtc
//
