LOCUS       sequence-410559-        7073 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  pDefence1:Eluc (defence-induced promoter with green-shifted 
            luciferase expression cassette).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7073)
  TITLE     Schwessinger Lab Plasmids 2023
REFERENCE   2  (bases 1 to 7073)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: pSW001
FEATURES             Location/Qualifiers
     source          1..7073
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     RBS             2307..2315
                     /label=Shine-Dalgarno sequence
                     /note="full consensus sequence for ribosome-binding sites 
                     upstream of start codons in E. coli; complementary to a 
                     region in the 3' end of the 16S rRNA (Chen et al., 1994)"
     terminator      2476..2728
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     misc_feature    2776..2800
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     CDS             complement(2956..4104)
                     /codon_start=1
                     /product="trans-acting replication protein that binds to 
                     and activates oriV"
                     /label=trfA
                     /translation="MNRTFDRKAYRQELIDAGFSAEDAETIASRTVMRAPRETFQSVGS
                     MVQQATAKIERDSVQLAPPALPAPSAAVERSRRLEQEAAGLAKSMTIDTRGTMTTKKRK
                     TAGEDLAKQVSEAKQAALLKHTKQQIKEMQLSLFDIAPWPDTMRAMPNDTARSALFTTR
                     NKKIPREALQNKVIFHVNKDVKITYTGVELRADDDELVWQQVLEYAKRTPIGEPITFTF
                     YELCQDLGWSINGRYYTKAEECLSRLQATAMGFTSDRVGHLESVSLLHRFRVLDRGKKT
                     SRCQVLIDEEIVVLFAGDHYTKFIWEKYRKLSPTARRMFDYFSSHREPYPLKLETFRLM
                     CGSDSTRVKKWREQVGEACEELRGSGLVEHAWVNDDLVHCKR"
     rep_origin      complement(4440..5028)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(4520..4539)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(5199..5990,5991..6059))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     5822..5841
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(6060..6164)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      6251..6882
                     /label=oriV
                     /note="incP origin of replication"
     misc_feature    6974..6998
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
ORIGIN
        1 tctgtgaaga caatgccgaa ttcggatccg gagaaggaga cgcagtagct acgttcctcc
       61 tgctgtcccc aggtggcgga caccgcgtga tcgacaacac cgagaggact tggcttgcac
      121 gccggccgcc gcggttcacg gcgccgtctt atttccttca gcgcgtccat gcaaatgcat
      181 gccgactata gttcgccata acccgaccaa ctaagcaaac ccttgctcga tcgacgtcgc
      241 cgacatggcg acatcctttc ccgtgcgtgc cttagccact aaggcgtcgc aatgtacaca
      301 ctagagctgg ctgccacata cgttctctct ttttgtcttg tcgtacgtat accatgtaaa
      361 gttacttttg gtggagtata cgcacgtaca tgcatggtgt tttttggggt gatgctccgt
      421 tcggttcggg tggagaaggt tcgcccgcgc ttcccctgta aatcacgtcc cgcgtgcaac
      481 gcaacggctt ccttgacagg ccaacaacgc tgttggttgg gagtcatcac gaaataaacg
      541 gcaaatgacg aggcgattaa ttcgtgcagc agctttgatc cgttcctagc tagtgtgatt
      601 tccaggaacc ttcccaagtt ctcctcatat atccagcccc ggccggctcc atcaaactca
      661 tcaaggaagg atttcgagca cccctgaccc tcccagctgg tgtagatccg tccatcgagt
      721 cctgcctacc taggagctct tcgctggtcg ccgcatccat tgatcccaac ctccatactt
      781 gttgtgtttc tcacccttct cgcttagctt tgcaatcgcg cgctgcacag cggaatggag
      841 agagagaaga acgtggtgta cggccccgag cccaagcacc ctctgggcaa cttcaccgcc
      901 ggcgagatgc tgtacaacgc tctgcacaag cactcccaca tcccccaggc catcctggac
      961 gtgatgggca acgagtccct ttcctaccag gagttcttcg acactactgt gaagctgggc
     1021 cagagcctcc agaactgtgg ctacaagatg aacgatgtcg tgtcgatctg tgcagagaac
     1081 aacaagagat tcttcatccc catcatctcc gcctggtaca tcggcatggt ggtggcccct
     1141 gtgaacgagg actatatccc agacgagctg tgtaaagtga ccggcatctc caagccgatc
     1201 ctggtcttta ccactaggaa gatcctgcct aaggttttgg aggttaaaga cagaaccaac
     1261 tacataaaga ggatcatcat actggactct gaagagaacc tgctgggctg cgagagcctg
     1321 cacaacttca tgtccaggta ctccgacaac aacctccaaa cattcaagcc tctgcactac
     1381 gaccctgtgg accaggtagc cgccatcctg tgctcctccg gcacaaccgg cctgcctaaa
     1441 ggcgtgatgc agacccacag gaacatctgt gtgagactca cacacgcatc tgaccccaga
     1501 gtgggtacac aactcatccc cggcgtatcc gtgctggcct acctgccatt cttccacgcc
     1561 ttcggcttca gtatcaacct gggctatttc atggtgggcc tgagagtggt gatgctccga
     1621 aggtttaacc aggaggtgtt cctgaaggcc atccaggact acgaggtgag gagcgtgatc
     1681 aacgttccct ccacaatcct gttcctgtcc aagagccctc tggtggacaa gtacgaccta
     1741 tccaccctgg cggagctgtg ctgtggagcc gctcctctgg cgaaggaggt ggccgagatc
     1801 gccgtgaaga ggctgaacct gccagggata cggtgtggct acggtctaac agagtctacc
     1861 tccgccaaca tccatactct gcacaacgag ttcaagtccg gctccctggg caaggtgaca
     1921 ccttacatgg ccgccaagat catcgacagg aacaccggcg aggccctggg tccaaaccag
     1981 gtgggcgagc tgtgcatctg gggacctatg gtaacaaaag gctatgtgaa caacccacag
     2041 gctactaagg aggccatcga cgacgacggc tggctgcact ctggcgactt cggctactac
     2101 gacgaggacg agtatttcta catcgtggac cggtacaagg agctgatcaa atacaagggc
     2161 tatcaggtcg cccctgtgga gctggaggag atcctccttc agcacccagg catcagggac
     2221 gtggccgtcg tgggtatccc tgacatcgag gccggcgagc tgccagccgg cttcgtggtg
     2281 aagcagcccg gcgcccaact caccgctaag gaggtgtacg acttcctggc ccagagggtg
     2341 tctcactcca agtacctgag gggcggcgta aggttcgtgg actctatccc caggaacgtg
     2401 acaggcaaga ttagtcgaaa agagctgagg gaggccctga tggagaaggc ttctaagctg
     2461 taagcttgtc aagcagatcg ttcaaacatt tggcaataaa gtttcttaag attgaatcct
     2521 gttgccggtc ttgcgatgat tatcatataa tttctgttga attacgttaa gcatgtaata
     2581 attaacatgt aatgcatgac gttatttatg agatgggttt ttatgattag agtcccgcaa
     2641 ttatacattt aatacgcgat agaaaacaaa atatagcgcg caaactagga taaattatcg
     2701 cgcgcggtgt catctatgtt actagatcga cgctgcaatt gtcttctgca cgaagtggtt
     2761 taaactatca gtgtttgaca ggatatattg gcgggtaaac ctaagagaaa agagcgttta
     2821 ttagaataat cggatattta aaagggcgtg aaaaggttta tccgttcgtc catttgtatg
     2881 tgcatgccaa ccacagggtt ccccagatca ggcgctggct gctgaacccc cagccggaac
     2941 tgaccccaca aggccctagc gtttgcaatg caccaggtca tcattgaccc aggcgtgttc
     3001 caccaggccg ctgcctcgca actcttcgca ggcttcgccg acctgctcgc gccacttctt
     3061 cacgcgggtg gaatccgatc cgcacatgag gcggaaggtt tccagcttga gcgggtacgg
     3121 ctcccggtgc gagctgaaat agtcgaacat ccgtcgggcc gtcggcgaca gcttgcggta
     3181 cttctcccat atgaatttcg tgtagtggtc gccagcaaac agcacgacga tttcctcgtc
     3241 gatcaggacc tggcaacggg acgttttctt gccacggtcc aggacgcgga agcggtgcag
     3301 cagcgacacc gattccaggt gcccaacgcg gtcggacgtg aagcccatcg ccgtcgcctg
     3361 taggcgcgac aggcattcct cggccttcgt gtaataccgg ccattgatcg accagcccag
     3421 gtcctggcaa agctcgtaga acgtgaaggt gatcggctcg ccgatagggg tgcgcttcgc
     3481 gtactccaac acctgctgcc acaccagttc gtcatcgtcg gcccgcagct cgacgccggt
     3541 gtaggtgatc ttcacgtcct tgttgacgtg gaaaatgacc ttgttttgca gcgcctcgcg
     3601 cgggattttc ttgttgcgcg tggtgaacag ggcagagcgg gccgtgtcgt ttggcatcgc
     3661 tcgcatcgtg tccggccacg gcgcaatatc gaacaaggaa agctgcattt ccttgatctg
     3721 ctgcttcgtg tgtttcagca acgcggcctg cttggcctcg ctgacctgtt ttgccaggtc
     3781 ctcgccggcg gtttttcgct tcttggtcgt catagttcct cgcgtgtcga tggtcatcga
     3841 cttcgccaaa cctgccgcct cctgttcaag acgacgcgaa cgctccacgg cggccgatgg
     3901 cgcgggcagg gcagggggag ccagttgcac gctgtcgcgc tcgatcttgg ccgtagcttg
     3961 ctggaccatc gagccgacgg actggaaggt ttcgcggggc gcacgcatga cggtgcggct
     4021 tgcgatggtt tcggcatcct cggcggaaaa ccccgcgtcg atcagttctt gcctgtatgc
     4081 cttccggtca aacgtccgat tcattcaccc tccttgcggg attgccccga ctcacgccgg
     4141 ggcaatgtgc ccttattcct gatttgaccc gcctggtgcc ttggtgtcca gataatccac
     4201 cttatcggca atgaagtcgg tcccgtagac cgtctggccg tccttctcgt acttggtatt
     4261 ccgaatcttg ccctgcacga ataccagcga ccccttgccc aaatacttgc cgtgggcctc
     4321 ggcctgagag ccaaaacact tgatgcggaa gaagtcggtg cgctcctgct tgtcgccggc
     4381 atcgttgcgc cacatctagg atctgccagg aaccgtaaaa aggccgcgtt gctggcgttt
     4441 ttccataggc tccgcccccc tgacgagcat cacaaaaatc gacgctcaag tcagaggtgg
     4501 cgaaacccga caggactata aagataccag gcgtttcccc ctggaagctc cctcgtgcgc
     4561 tctcctgttc cgaccctgcc gcttaccgga tacctgtccg cctttctccc ttcgggaagc
     4621 gtggcgcttt ctcatagctc acgctgtagg tatctcagtt cggtgtaggt cgttcgctcc
     4681 aagctgggct gtgtgcacga accccccgtt cagcccgacc gctgcgcctt atccggtaac
     4741 tatcgtcttg agtccaaccc ggtaagacac gacttatcgc cactggcagc agccactggt
     4801 aacaggatta gcagagcgag gtatgtaggc ggtgctacag agttcttgaa gtggtggcct
     4861 aactacggct acactagaag aacagtattt ggtatctgcg ctctgctgaa gccagttacc
     4921 ttcggaaaaa gagttggtag ctcttgatcc ggcaaacaaa ccaccgctgg tagcggtggt
     4981 ttttttgttt gcaagcagca gattacgcgc agaaaaaaag gatctcaaga agatcctttg
     5041 atcttttcta cggggtctga cgctcagtgg aacgaaaact cacgttaagg gattttggtc
     5101 atgagattat caaaaaggat cttcacctag atccttttaa attaaaaatg aagttttaaa
     5161 tcaatctaaa gtatatatga gtaaacttgg tctgacagtt accaatgctt aatcagtgag
     5221 gcacctatct cagcgatctg tctatttcgt tcatccatag ttgcctgact ccccgtcgtg
     5281 tagataacta cgatacggga gggcttacca tctggcccca gtgctgcaat gataccgcga
     5341 gaaccacgct caccggctcc agatttatca gcaataaacc agccagccgg aagggccgag
     5401 cgcagaagtg gtcctgcaac tttatccgcc tccatccagt ctattaattg ttgccgggaa
     5461 gctagagtaa gtagttcgcc agttaatagt ttgcgcaacg ttgttgccat tgctacaggc
     5521 atcgtggtgt cacgctcgtc gtttggtatg gcttcattca gctccggttc ccaacgatca
     5581 aggcgagtta catgatcccc catgttgtgc aaaaaagcgg ttagctcctt cggtcctccg
     5641 atcgttgtca gaagtaagtt ggccgcagtg ttatcactca tggttatggc agcactgcat
     5701 aattctctta ctgtcatgcc atccgtaaga tgcttttctg tgactggtga gtactcaacc
     5761 aagtcattct gagaatagtg tatgcggcga ccgagttgct cttgcccggc gtcaatacgg
     5821 gataataccg cgccacatag cagaacttta aaagtgctca tcattggaaa acgttcttcg
     5881 gggcgaaaac tctcaaggat cttaccgctg ttgagatcca gttcgatgta acccactcgt
     5941 gcacccaact gatcttcagc atcttttact ttcaccagcg tttctgggtg agcaaaaaca
     6001 ggaaggcaaa atgccgcaaa aaagggaata agggcgacac ggaaatgttg aatactcata
     6061 ctcttccttt ttcaatatta ttgaagcatt tatcaaggtt attgtctcat gagcggatac
     6121 atatttgaat gtatttagaa aaataaacaa ataggggttc cgcgcacgaa ttggccagcg
     6181 ctgccatttt tggggtgagg ccgttcgcgg ccgaggggcg cagcccctgg ggggatggga
     6241 ggcccgcgtt agcgggccgg gagggttcga gaaggggggg cacccccctt cggcgtgcgc
     6301 ggtcacgcgc acagggcgca gccctggtta aaaacaaggt ttataaatat tggtttaaaa
     6361 gcaggttaaa agacaggtta gcggtggccg aaaaacgggc ggaaaccctt gcaaatgctg
     6421 gattttctgc ctgtggacag cccctcaaat gtcaataggt gcgcccctca tctgtcagca
     6481 ctctgcccct caagtgtcaa ggatcgcgcc cctcatctgt cagtagtcgc gcccctcaag
     6541 tgtcaatacc gcagggcact tatccccagg cttgtccaca tcatctgtgg gaaactcgcg
     6601 taaaatcagg cgttttcgcc gatttgcgag gctggccagc tccacgtcgc cggccgaaat
     6661 cgagcctgcc cctcatctgt caacgccgcg ccgggtgagt cggcccctca agtgtcaacg
     6721 tccgcccctc atctgtcagt gagggccaag ttttccgcga ggtatccaca acgccggcgg
     6781 ccgcggtgtc tcgcacacgg cttcgacggc gtttctggcg cgtttgcagg gccatagacg
     6841 gccgccagcc cagcggcgag ggcaaccagc ccggtgagcg tcgcaaagga gatcctgatc
     6901 tgactgatgg gctgcctgta tcgagtggtg attttgtgcc gagctgccgg tcggggagct
     6961 gttggctggc tggtggcagg atatattgtg gtgtaaacaa attgacgctt agacaactta
     7021 ataacacatt gcggacgttt ttaatgtact ggggtggatg cagtgggccc cac
//
