LOCUS       sequence-410597-       10099 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  tetO-OS*K (Dox-inducible reprogramming lentiviral OSK vector 
            expressing mouse Oct4 + super-Sox + Klf4).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10099)
  AUTHORS   MacCarthy CM, Wu G, Malik V, Menuchin-Lasowski Y, Velychko T, Keshet
            G, Fan R, Bedzhov I, Church GM, Jauch R, Cojocaru V, Scholer HR, 
            Velychko S
  TITLE     Highly cooperative chimeric super-SOX induces naive pluripotency 
            across species.
  JOURNAL   Cell Stem Cell. 2024 Jan 4;31(1):127-147.e9. doi: 
            10.1016/j.stem.2023.11.010. Epub 2023 Dec 22.
  PUBMED    38141611
REFERENCE   2  (bases 1 to 10099)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.stem.2023.11.010"; journalName: "Cell Stem Cell"; date: 
            "2024-01-4- 4"; volume: "31"; issue: "1"; pages: "127-147.e9"
COMMENT     Sequence Label: pHAGE2-TetOminiCMV-Oct4-p2a-Sox2-17-t2a-Klf4
FEATURES             Location/Qualifiers
     source          1..10099
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     LTR             1..634
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     misc_feature    681..806
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1303..1536
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1721..1765
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    2058..2175
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     protein_bind    join(2251..2269,2270..2292,2293..2311,2312..2334,2335..2353,
                     2354..2376,2377..2395,2396..2418,2419..2437,2438..2460,
                     2461..2479,2480..2502,2503..2521)
                     /label=tetracycline response element
                     /bound_moiety="tetracycline repressor TetR"
                     /note="contains seven copies of the tetracycline operator 
                     tetO"
     primer_bind     2589..2613
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     regulatory      2679..2688
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             3756..3812
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
     CDS             5016..5069
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
     misc_feature    6541..7129
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(6594..6614)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(7012..7023)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             7277..7457
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     primer_bind     complement(7504..7522)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        7590..7694
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(7695..7763,7764..8555)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(7913..7932)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      8726..9314
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     9468..9485
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    9725..9746
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(9761..9766,9767..9784,9785..9791)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    9799..9815
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     9804..9826
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     9823..9839
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     9823..9839
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     9889..9908
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
ORIGIN
        1 tggaagggct aattcactcc caaagaagac aagatatcct tgatctgtgg atctaccaca
       61 cacaaggcta cttccctgat tagcagaact acacaccagg gccaggggtc agatatccac
      121 tgacctttgg atggtgctac aagctagtac cagttgagcc agataaggta gaagaggcca
      181 ataaaggaga gaacaccagc ttgttacacc ctgtgagcct gcatgggatg gatgacccgg
      241 agagagaagt gttagagtgg aggtttgaca gccgcctagc atttcatcac gtggcccgag
      301 agctgcatcc ggagtacttc aagaactgct gatatcgagc ttgctacaag ggactttccg
      361 ctggggactt tccagggagg cgtggcctgg gcgggactgg ggagtggcga gccctcagat
      421 cctgcatata agcagctgct ttttgcctgt actgggtctc tctggttaga ccagatctga
      481 gcctgggagc tctctggcta actagggaac ccactgctta agcctcaata aagcttgcct
      541 tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta gagatccctc
      601 agaccctttt agtcagtgtg gaaaatctct agcagtggcg cccgaacagg gacttgaaag
      661 cgaaagggaa accagaggag ctctctcgac gcaggactcg gcttgctgaa gcgcgcacgg
      721 caagaggcga ggggcggcga ctggtgagta cgccaaaaat tttgactagc ggaggctaga
      781 aggagagaga tgggtgcgag agcgtcagta ttaagcgggg gagaattaga tcgcgatggg
      841 aaaaaattcg gttaaggcca gggggaaaga aaaaatataa attaaaacat atagtatggg
      901 caagcaggga gctagaacga ttcgcagtta atcctggcct gttagaaaca tcagaaggct
      961 gtagacaaat actgggacag ctacaaccat cccttcagac aggatcagaa gaacttagat
     1021 cattatataa tacagtagca accctctatt gtgtgcatca aaggatagag ataaaagaca
     1081 ccaaggaagc tttagacaag atagaggaag agcaaaacaa aagtaagacc accgcacagc
     1141 aagcggccgg ccgctgatct tcagacctgg aggaggagat atgagggaca attggagaag
     1201 tgaattatat aaatataaag tagtaaaaat tgaaccatta ggagtagcac ccaccaaggc
     1261 aaagagaaga gtggtgcaga gagaaaaaag agcagtggga ataggagctt tgttccttgg
     1321 gttcttggga gcagcaggaa gcactatggg cgcagcgtca atgacgctga cggtacaggc
     1381 cagacaatta ttgtctggta tagtgcagca gcagaacaat ttgctgaggg ctattgaggc
     1441 gcaacagcat ctgttgcaac tcacagtctg gggcatcaag cagctccagg caagaatcct
     1501 ggctgtggaa agatacctaa aggatcaaca gctcctgggg atttggggtt gctctggaaa
     1561 actcatttgc accactgctg tgccttggaa tgctagttgg agtaataaat ctctggaaca
     1621 gatttggaat cacacgacct ggatggagtg ggacagagaa attaacaatt acacaagctt
     1681 aatacactcc ttaattgaag aatcgcaaaa ccagcaagaa aagaatgaac aagaattatt
     1741 ggaattagat aaatgggcaa gtttgtggaa ttggtttaac ataacaaatt ggctgtggta
     1801 tataaaatta ttcataatga tagtaggagg cttggtaggt ttaagaatag tttttgctgt
     1861 actttctata gtgaatagag ttaggcaggg atattcacca ttatcgtttc agacccacct
     1921 cccaaccccg aggggacccg acaggcccga aggaatagaa gaagaaggtg gagagagaga
     1981 cagagacaga tccattcgat tagtgaacgg atctcgacgg tatcgccgaa ttcacaaatg
     2041 gcagtattca tccacaattt taaaagaaaa ggggggattg gggggtacag tgcaggggaa
     2101 agaatagtag acataatagc aacagacata caaactaaag aattacaaaa acaaattaca
     2161 aaaattcaaa attttcgggt ttattacagg gacagcagag atccagtttg gactagtcca
     2221 caccctaact gacacactcg agtttaccac tccctatcag tgatagagaa aagtgaaagt
     2281 cgagtttacc actccctatc agtgatagag aaaagtgaaa gtcgagttta ccactcccta
     2341 tcagtgatag agaaaagtga aagtcgagtt taccactccc tatcagtgat agagaaaagt
     2401 gaaagtcgag tttaccactc cctatcagtg atagagaaaa gtgaaagtcg agtttaccac
     2461 tccctatcag tgatagagaa aagtgaaagt cgagtttacc actccctatc agtgatagag
     2521 aaaagtgaaa gtcgagctcg gtacccgggt cgaggtaggc atgtacggtg ggaggcctat
     2581 ataagcagag ctcgtttagt gaaccgtcag atcgcctgga gacgccatcc acgctgtttt
     2641 gacctccata gaagacaccg ggaccgatcc agcctgcggc cgccatggct ggacacctgg
     2701 cttcagactt cgccttctca cccccaccag gtgggggtga tgggtcagca gggctggagc
     2761 cgggctgggt ggatcctcga acctggctaa gcttccaagg gcctccaggt gggcctggaa
     2821 tcggaccagg ctcagaggta ttggggatct ccccatgtcc gcccgcatac gagttctgcg
     2881 gagggatggc atactgtgga cctcaggttg gactgggcct agtcccccaa gttggcgtgg
     2941 agactttgca gcctgagggc caggcaggag cacgagtgga aagcaactca gagggaacct
     3001 cctctgagcc ctgtgccgac cgccccaatg ccgtgaagtt ggagaaggtg gaaccaactc
     3061 ccgaggagtc ccaggacatg aaagccctgc agaaggagct agaacagttt gccaagctgc
     3121 tgaagcagaa gaggatcacc ttggggtaca cccaggccga cgtggggctc accctgggcg
     3181 ttctctttgg aaaggtgttc agccagacca ccatctgtcg cttcgaggcc ttgcagctca
     3241 gccttaagaa catgtgtaag ctgcggcccc tgctggagaa gtgggtggag gaagccgaca
     3301 acaatgagaa ccttcaggag atatgcaaat cggagaccct ggtgcaggcc cggaagagaa
     3361 agcgaactag cattgagaac cgtgtgaggt ggagtctgga gaccatgttt ctgaagtgcc
     3421 cgaagccctc cctacagcag atcactcaca tcgccaatca gcttgggcta gagaaggatg
     3481 tggttcgagt atggttctgt aaccggcgcc agaagggcaa aagatcaagt attgagtatt
     3541 cccaacgaga agagtatgag gctacaggga cacctttccc agggggggct gtatcctttc
     3601 ctctgccccc aggtccccac tttggcaccc caggctatgg aagcccccac ttcaccacac
     3661 tctactcagt cccttttcct gagggcgagg cctttccctc tgttcccgtc actgctctgg
     3721 gctctcccat gcattcaaac gctagcggca gcggcgccac gaacttctct ctgttaaagc
     3781 aagcaggaga tgttgaagaa aaccccgggc ctgcatgcat gtataacatg atggagacgg
     3841 agctgaagcc gccgggcccg cagcaagctt cggggggcgg cggcggagga ggcaacgcca
     3901 cggcggcggc gaccggcggc aaccagaaga acagcccgga ccgcgtcaag aggcccatga
     3961 acgccttcat ggtatggtcc cgggggcagc ggcgtaagat ggcccaggag aaccccaaga
     4021 tgcacaactc ggagatcagc aagcgcctgg gcgcggagtg gaaagcgttg accttggcag
     4081 agaagcggcc gttcatcgac gaggccaagc ggctgcgcgt gctgcatatg caggaccacc
     4141 ccaactacaa gtaccggccg cggcggcgca agcaggtgaa gcgcatgaag cgggtggagg
     4201 gaggcttcct gcacgctctc gtcgagcccc aggccggcgc gcttggtccc gagggcggcc
     4261 gcgtggccat ggatggcctg ggtctgcctt tcccggagcc gggctatccg gccggtcctc
     4321 cgctgatgtc tccgcacatg ggcccccact atcgggactg ccagggactg ggcgctcccg
     4381 cgctcgacgg ctaccctctg cccactccgg acacatcccc gctggatggc gtggagcagg
     4441 acccggcttt ctttgcagcc ccgctgccag gggactgccc ggcggccggc acctacactt
     4501 acgctccagt ctcggactat gcagtgtccg tagagccgcc cgctggcccc atgcgagtgg
     4561 ggccggaccc ctcgggccct gcgatgccgg ggatcctggc gccccccagc gctctgcacc
     4621 tgtactacgg cgcgatgggc tcgcccgccg caagtgcggg gcgcggtttc cacgcgcaac
     4681 cccagcagcc gctgcaaccg caggcaccgc ccccgccacc gcagcagcag cacccagcgc
     4741 acggccccgg gcaaccttcg ccccctcccg aggctctgcc ctgccgggat ggcacggaat
     4801 ccaaccagcc cactgagctc ctaggggagg tggaccgcac ggaatttgaa cagtatctgc
     4861 cctttgtgta taagcccgag atgggtcttc cctaccaggg acacgactgc ggagtgaacc
     4921 tctcagacag ccacggagcc atttcctccg tggtgtccga cgctagctca gcggtctact
     4981 attgcaacta ccccgacatt gcatgcggct ccggcgaggg caggggaagt cttctaacat
     5041 gcggggacgt ggaggaaaat cccggcccac tcgagatgag gcagccacct ggcgagtctg
     5101 acatggctgt cagcgacgct ctgctcccgt ccttctccac gttcgcgtcc ggcccggcgg
     5161 gaagggagaa gacactgcgt ccagcaggtg ccccgactaa ccgttggcgt gaggaactct
     5221 ctcacatgaa gcgacttccc ccacttcccg gccgccccta cgacctggcg gcgacggtgg
     5281 ccacagacct ggagagtggc ggagctggtg cagcttgcag cagtaacaac ccggccctcc
     5341 tagcccggag ggagaccgag gagttcaacg acctcctgga cctagacttt atcctttcca
     5401 actcgctaac ccaccaggaa tcggtggccg ccaccgtgac cacctcggcg tcagcttcat
     5461 cctcgtcttc cccagcgagc agcggccctg ccagcgcgcc ctccacctgc agcttcagct
     5521 atccgatccg ggccgggggt gacccgggcg tggctgccag caacacaggt ggagggctcc
     5581 tctacagccg agaatctgcg ccacctccca cggccccctt caacctggcg gacatcaatg
     5641 acgtgagccc ctcgggcggc ttcgtggctg agctcctgcg gccggagttg gacccagtat
     5701 acattccgcc acagcagcct cagccgccag gtggcgggct gatgggcaag tttgtgctga
     5761 aggcgtctct gaccacccct ggcagcgagt acagcagccc ttcggtcatc agtgttagca
     5821 aaggaagccc agacggcagc caccccgtgg tagtggcgcc ctacagcggt ggcccgccgc
     5881 gcatgtgccc caagattaag caagaggcgg tcccgtcctg cacggtcagc cggtccctag
     5941 aggcccattt gagcgctgga ccccagctca gcaacggcca ccggcccaac acacacgact
     6001 tccccctggg gcggcagctc cccaccagga ctacccctac actgagtccc gaggaactgc
     6061 tgaacagcag ggactgtcac cctggcctgc ctcttccccc aggattccat ccccatccgg
     6121 ggcccaacta ccctcctttc ctgccagacc agatgcagtc acaagtcccc tctctccatt
     6181 atcaagagct catgccaccg ggttcctgcc tgccagagga gcccaagcca aagaggggaa
     6241 gaaggtcgtg gccccggaaa agaacagcca cccacacttg tgactatgca ggctgtggca
     6301 aaacctatac caagagttct catctcaagg cacacctgcg aactcacaca ggcgagaaac
     6361 cttaccactg tgactgggac ggctgtgggt ggaaattcgc ccgctcggat gaactgacca
     6421 ggcactaccg caaacacaca gggcaccggc cctttcagtg ccagaagtgc gacagggcct
     6481 tttccaggtc ggaccacctt gccttacaca tgaagaggca cttttaaatc gatagatcct
     6541 aatcaacctc tggattacaa aatttgtgaa agattgactg gtattcttaa ctatgttgct
     6601 ccttttacgc tatgtggata cgctgcttta atgcctttgt atcatgctat tgcttcccgt
     6661 atggctttca ttttctcctc cttgtataaa tcctggttgc tgtctcttta tgaggagttg
     6721 tggcccgttg tcaggcaacg tggcgtggtg tgcactgtgt ttgctgacgc aacccccact
     6781 ggttggggca ttgccaccac ctgtcagctc ctttccggga ctttcgcttt ccccctccct
     6841 attgccacgg cggaactcat cgccgcctgc cttgcccgct gctggacagg ggctcggctg
     6901 ttgggcactg acaattccgt ggtgttgtcg gggaaatcat cgtcctttcc ttggctgctc
     6961 gcctgtgttg ccacctggat tctgcgcggg acgtccttct gctacgtccc ttcggccctc
     7021 aatccagcgg accttccttc ccgcggcctg ctgccggctc tgcggcctct tccgcgtctt
     7081 cgccttcgcc ctcagacgag tcggatctcc ctttgggccg cctccccgcc tggtaccttt
     7141 aagaccaatg acttacaagg cagctgtaga tcttagccac tttttaaaag aaaagggggg
     7201 actggaaggg ctaattcact cccaacgaag acaagatcac ctgcaggaca ggcgcgccct
     7261 gctttttgct tgtactgggt ctctctggtt agaccagatc tgagcctggg agctctctgg
     7321 ctaactaggg aacccactgc ttaagcctca ataaagcttg ccttgagtgc ttcaagtagt
     7381 gtgtgcccgt ctgttgtgtg actctggtaa ctagagatcc ctcagaccct tttagtcagt
     7441 gtggaaaatc tctagcaccc gggcgattaa ggaaagggct agatcattct tgaagacgaa
     7501 agggcctcgt gatacgccta tttttatagg ttaatgtcat gataataatg gtttcttaga
     7561 cgtcaggtgg cacttttcgg ggaaatgtgc gcggaacccc tatttgttta tttttctaaa
     7621 tacattcaaa tatgtatccg ctcatgagac aataaccctg ataaatgctt caataatatt
     7681 gaaaaaggaa gagtatgagt attcaacatt tccgtgtcgc ccttattccc ttttttgcgg
     7741 cattttgcct tcctgttttt gctcacccag aaacgctggt gaaagtaaaa gatgctgaag
     7801 atcagttggg tgcacgagtg ggttacatcg aactggatct caacagcggt aagatccttg
     7861 agagttttcg ccccgaagaa cgttttccaa tgatgagcac ttttaaagtt ctgctatgtg
     7921 gcgcggtatt atcccgtgtt gacgccgggc aagagcaact cggtcgccgc atacactatt
     7981 ctcagaatga cttggttgag tactcaccag tcacagaaaa gcatcttacg gatggcatga
     8041 cagtaagaga attatgcagt gctgccataa ccatgagtga taacactgcg gccaacttac
     8101 ttctgacaac gatcggagga ccgaaggagc taaccgcttt tttgcacaac atgggggatc
     8161 atgtaactcg ccttgatcgt tgggaaccgg agctgaatga agccatacca aacgacgagc
     8221 gtgacaccac gatgcctgta gcaatggcaa caacgttgcg caaactatta actggcgaac
     8281 tacttactct agcttcccgg caacaattaa tagactggat ggaggcggat aaagttgcag
     8341 gaccacttct gcgctcggcc cttccggctg gctggtttat tgctgataaa tctggagccg
     8401 gtgagcgtgg gtctcgcggt atcattgcag cactggggcc agatggtaag ccctcccgta
     8461 tcgtagttat ctacacgacg gggagtcagg caactatgga tgaacgaaat agacagatcg
     8521 ctgagatagg tgcctcactg attaagcatt ggtaactgtc agaccaagtt tactcatata
     8581 tactttagat tgatttaaaa cttcattttt aatttaaaag gatctaggtg aagatccttt
     8641 ttgataatct catgaccaaa atcccttaac gtgagttttc gttccactga gcgtcagacc
     8701 ccgtagaaaa gatcaaagga tcttcttgag atcctttttt tctgcgcgta atctgctgct
     8761 tgcaaacaaa aaaaccaccg ctaccagcgg tggtttgttt gccggatcaa gagctaccaa
     8821 ctctttttcc gaaggtaact ggcttcagca gagcgcagat accaaatact gttcttctag
     8881 tgtagccgta gttaggccac cacttcaaga actctgtagc accgcctaca tacctcgctc
     8941 tgctaatcct gttaccagtg gctgctgcca gtggcgataa gtcgtgtctt accgggttgg
     9001 actcaagacg atagttaccg gataaggcgc agcggtcggg ctgaacgggg ggttcgtgca
     9061 cacagcccag cttggagcga acgacctaca ccgaactgag atacctacag cgtgagctat
     9121 gagaaagcgc cacgcttccc gaagggagaa aggcggacag gtatccggta agcggcaggg
     9181 tcggaacagg agagcgcacg agggagcttc cagggggaaa cgcctggtat ctttatagtc
     9241 ctgtcgggtt tcgccacctc tgacttgagc gtcgattttt gtgatgctcg tcaggggggc
     9301 ggagcctatg gaaaaacgcc agcaacgcgg cctttttacg gttcctggcc ttttgctggc
     9361 cttttgctca catgttcttt cctgcgttat cccctgattc tgtggataac cgtattaccg
     9421 cctttgagtg agctgatacc gctcgccgca gccgaacgac cgagcgcagc gagtcagtga
     9481 gcgaggaagc ggaagagcgc ccaatacgca aaccgcctct ccccgcgcgt tggccgattc
     9541 attaatgcag caagctcatg gctgactaat tttttttatt tatgcagagg ccgaggccgc
     9601 ctcggcctct gagctattcc agaagtagtg aggaggcttt tttggaggcc taggcttttg
     9661 caaaaagctc cccgtggcac gacaggtttc ccgactggaa agcgggcagt gagcgcaacg
     9721 caattaatgt gagttagctc actcattagg caccccaggc tttacacttt atgcttccgg
     9781 ctcgtatgtt gtgtggaatt gtgagcggat aacaatttca cacaggaaac agctatgaca
     9841 tgattacgaa tttcacaaat aaagcatttt tttcactgca ttctagttgt ggtttgtcca
     9901 aactcatcaa tgtatcttat catgtctgga tcaactggat aactcaagct aaccaaaatc
     9961 atcccaaact tcccacccca taccctatta ccactgccaa ttacctgtgg tttcatttac
    10021 tctaaacctg tgattcctct gaattatttt cattttaaag aaattgtatt tgttaaatat
    10081 gtactacaaa cttagtagt
//
