LOCUS       sequence-410621-         276 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  In vitro expression of polyProline FETCH reporter from the T7 
            promoter.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 276)
  AUTHORS   Willi JA, Karim AS, Jewett MC
  TITLE     Cell-Free Translation Quantification via a Fluorescent Minihelix.
  JOURNAL   ACS Synth Biol. 2024 Jul 19;13(7):2253-2259. doi: 
            10.1021/acssynbio.4c00266. Epub 2024 Jul 9.
  PUBMED    38979618
REFERENCE   2  (bases 1 to 276)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1021/acssynbio.4c00266"; journalName: "ACS Synth Biol"; date: 
            "2024-07-19- 19"; volume: "13"; issue: "7"; pages: "2253-2259"
COMMENT     Sequence Label: pJL1-sFLAG-p5-PRB4_R7-9-FETCH1
FEATURES             Location/Qualifiers
     source          1..276
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             247..264
                     /codon_start=1
                     /product="tetracysteine peptide that binds biarsenical 
                     labeling reagents"
                     /label=tetracysteine tag
                     /translation="CCPGCC"
ORIGIN
        1 atggactaca aagacgaaga cctgctgccg ccccctcctc ctatcccaaa gcctgaagga
       61 cgaccccctc agggtgggaa ccaatcgcag ggaccacccc cgcatcccgg aaaaccagag
      121 agaccaccgc ctcaaggtgg caatcagtct cacagaccac ccccaccacc cggaaaacct
      181 gaaagacccc cacctcaagg cggtaaccaa tcccaaggtc cgccccctca tccgggcttt
      241 ttaaactgct gtcctgggtg ttgtatggaa ccgtaa
//
