LOCUS       sequence-410623-         264 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  In vitro expression of polyProline FETCH reporter from the T7 
            promoter.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 264)
  AUTHORS   Willi JA, Karim AS, Jewett MC
  TITLE     Cell-Free Translation Quantification via a Fluorescent Minihelix.
  JOURNAL   ACS Synth Biol. 2024 Jul 19;13(7):2253-2259. doi: 
            10.1021/acssynbio.4c00266. Epub 2024 Jul 9.
  PUBMED    38979618
REFERENCE   2  (bases 1 to 264)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1021/acssynbio.4c00266"; journalName: "ACS Synth Biol"; date: 
            "2024-07-19- 19"; volume: "13"; issue: "7"; pages: "2253-2259"
COMMENT     Sequence Label: pJL1-sFLAG-p1-PRB4_R7-9-FETCH1
FEATURES             Location/Qualifiers
     source          1..264
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             235..252
                     /codon_start=1
                     /product="tetracysteine peptide that binds biarsenical 
                     labeling reagents"
                     /label=tetracysteine tag
                     /translation="CCPGCC"
ORIGIN
        1 atggactaca aagacgaaga cctgctgccg atcccaaagc ctgaaggacg accccctcag
       61 ggtgggaacc aatcgcaggg accacccccg catcccggaa aaccagagag accaccgcct
      121 caaggtggca atcagtctca cagaccaccc ccaccacccg gaaaacctga aagaccccca
      181 cctcaaggcg gtaaccaatc ccaaggtccg ccccctcatc cgggcttttt aaactgctgt
      241 cctgggtgtt gtatggaacc gtaa
//
