LOCUS       sequence-410654-        8215 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses the protein of wmiRFP670-2-T2A-HO1 in neurons of C. 
            elegans.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8215)
  AUTHORS   Zhang H, Papadaki S, Sun X, Wang X, Drobizhev M, Yao L, Rehbock M, 
            Koster RW, Wu L, Namikawa K, Piatkevich KD
  TITLE     Quantitative assessment of near-infrared fluorescent proteins.
  JOURNAL   Nat Methods. 2023 Sep 4. doi: 10.1038/s41592-023-01975-z.
  PUBMED    37666982
REFERENCE   2  (bases 1 to 8215)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Methods. 2023 Sep 4. doi: 10.1038/s41592-023-01975-z."
COMMENT     Sequence Label: pSF11-wmiRFP670-2-T2A-HO1
FEATURES             Location/Qualifiers
     source          1..8215
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             3893..3946
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
     primer_bind     complement(5785..5807)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     5907..5926
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     complement(6008..6026)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        6094..6198
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(6199..6267,6268..7059)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(6417..6436)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      7230..7818
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     7719..7738
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     7972..7989
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    8106..8127
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(8142..8147,8148..8165,8166..8172)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    8180..8196
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     8185..8207
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     join(8204..8215,1..5)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     join(8204..8215,1..5)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
ORIGIN
        1 atgaccatga ttacgccaag cttgcatgcg cggccgcact gactgggccg gccggatcca
       61 taggtatgcc tgtcattctc tatttttaaa cgttcctgca gcagtagcac aacagagcca
      121 gcagttttcc tcttcttctc catcttcttc tgcttcttcc tctcttccca aaaaaaaaaa
      181 aaaaaaaaaa agtgaggggg ctccctgccc ccccccccct cccccccccc tcttctcctt
      241 gaagctcatc cagacgtcgc tgtcctcctc ctcctcgtcc ccggggcgct gtgtgtgtgt
      301 gtgtgtgctc cgtgagcatg tagcagcttc agcccattta aaggcgcaca ctctctgcat
      361 ctctgcgtgt ctcgacactc gttttttctc ttcttttctg ctgcacacac acacacacac
      421 acacacacgc tcatgccaga gcacttcttg ctccgggggg ctccgcgcgg agcccgagag
      481 gacctcctcc tcctttttct tcttcgcttt ttagttcttc ttgcctcggt ctcgcccctt
      541 gttttgctct ccatgtagta tatacatctg ggtgtgtgtg tgtgtgtgca cctttggagc
      601 attttttgaa cctttccaga actttggagc aacattttca gcttgttcag tgcacttttt
      661 aggctgaaaa tcctattttt aggttttcga aagttttttt ggagcatttt tcgtgcaatt
      721 taggtgcaaa attaaaaaaa agggttttgt agtgaattaa aatttttttg aaactttcgc
      781 ctttttaaaa aaatgtttct aatatcttta aaaattggaa tattttggaa aacaatttgt
      841 tttcaattgg caatttatta attctgagct tctgagcgta aatttttttt tattttggag
      901 caattctgag atgtcttagt gcatttttcg ggcatacata aaaaaaaatt tatcttaata
      961 ataaaaacct taaatttagg cttcaccgtc tggaaattca attttttcag ctgaaaaact
     1021 ttcaaaaaaa aaaaaattaa attttcacat ttattttctc tggaattttg gagcaatttc
     1081 aaagctgtct tagtgcattt ttagtacaca aatatttttt catttttctt aaattttaat
     1141 aatctaagct taaataaagg cttcaccgtc tgaaaatgca acttttttca gatgaaatat
     1201 taaaaaaaaa acctctttta ttaattttgt gctccaaact ttcccacggt acgcaaatca
     1261 tgtaatatgt acttttaaac attatttttc caatcaaaaa tatccaacag cttttcctta
     1321 caatttgaaa atttttatta aaatttcaaa aaaatttttt ttttttttaa tttcgaattt
     1381 ttttaaactt gttgtaactt ttttccctgt cggcgccaat taacaaaata cgtagaattt
     1441 tgcgcttatt gttttgttag ttaacaaatt tttgacaact ttcttatcag ctgagattta
     1501 ggtgtttcag ttcgcaatct gcgtctctcc ttgctctcct ctattttaaa acccccataa
     1561 ctttgttctt gtgagagtta tcaaaaaatt gtcaactaac aaaatataga gcacaaaaat
     1621 atgcacattt tgtatgttga caattttttg ataccttttt tagttttcga gatatcttag
     1681 ttttagttta gctgtacctt tttctggcaa aaaacttcca aatttcggca atttttcggc
     1741 aaatttcccg gcaaatttcg ccaagtacat cgggctctca gacaagtggt ctgtgagtgg
     1801 aagtcgataa aagtagttta cgtgctctcc gtccccccgc ttcttctccg tctctgcttc
     1861 tatttctcca ttgactagtc ccactacttt ttacggggcg gagggctctc ttgcactcca
     1921 gcttcttctc tttttgtgct cttttttgcc cgtatgtggg tgctcattta tttttctttg
     1981 gttttaaaat aaattcggtt tattaggcta ctcgtaaaaa tgaagaaaaa acttcaaatt
     2041 caaacgacat taaaaaaaaa tttaataaaa attttttgaa ttgctcaaaa gccccaccta
     2101 tatatatata tagctccgcc tacttctcgc acagaccccg cccacttttt gactccgccc
     2161 ccttattggc aaaagttctt gaaaaaaata aaaataaaaa ttcaaattga aacaattttt
     2221 ttttaatgaa gtagtttttt tctaaattga tgaaaatttc taaacagttt acaagcaccg
     2281 cccacttttt gactccgccc ctttgttgga aaaattcttg taaaaaaaaa taaaaaataa
     2341 aagcttctct ttcaaacgat ttttagttgg tctttgtatg aaaagttttt ttcctaaaat
     2401 agaaatgtct gaataatttg caagctccgc ccttttctta cccagactcc gcccctttat
     2461 tgactaattt ttttttgtcc atttacctga agttcaaaaa aaaaatttac agtttcatag
     2521 agtaataatt tttcaaaaat ctctttttcc aatgtttaat gtgtcattat aaacccgccc
     2581 agttttaagc tccgcccatt taggccccgc ccatttatga cccaaatatt tttttctcta
     2641 ttcacccgtg gatttccctc caacaagtta ccccaaaaat aaaaataaaa attcctgcag
     2701 gcgcccaccg ggatccggcg cgcctctaga ggatccccgg gattggccaa aggacccaaa
     2761 ggtatgtttc gaatgatact aacataacat agaacatttt caggaggacc cttggctagc
     2821 gtcgacggta ccaaaaatgg cccgtaaggt cgaccttacc tcctgcgacc gcgagccaat
     2881 ccacatccca ggatccatcc agccatgcgg atgccttctt gcctgcgacg cccaagccgt
     2941 ccgcatcacc cgcatcaccg agaacgccgg agccttcttc ggacgcgaga ccccacgtgt
     3001 cggagagctt cttgccgact acttcggaga gaccgaggcc cacgcccttc gtaacgccct
     3061 tgcccaatcc tccgacccaa agcgtccagc ccttatcttc ggatggcgcg acggacttac
     3121 cggacgcacc ttcgacatct cccttcaccg tcacgacgga acctccatca tcgagttcga
     3181 gccagccgcc gccgagcaag ccgacaaccc acttcgcctt acccgccaaa tcatcgcccg
     3241 caccaaggta agtttaaaca tatatatact aactaaccct gattatttaa attttcagga
     3301 gcttaagtcc cttgaggaga tggccgcccg tgtcccacgt taccttcaag ccatgcttgg
     3361 ataccaccgt gtcatgcttt accgtttcgc cgacgacgga tccggaatgg tcatcggaga
     3421 ggccaagcgt tccgaccttg agtccttcct tggacaacac ttcccagcct cccttgtccc
     3481 acaacaagcc cgtcttcttt accttaaggt aagtttaaac agttcggtac taactaacca
     3541 tacatattta aattttcaga acgccatccg tgtcgtctcc gactcccgtg gaatctcctc
     3601 ccgtatcgtc ccagagcacg acgcctccgg agccgccctt gacctttcct tcgcccacct
     3661 tcgttccatc tccccatgcc accttgagtt ccttcgcaac atgggagtct ccgcctccat
     3721 gtccctttcc atcatcatcg acggaaccct ttggggactt atcatctgcc accactacga
     3781 gccacgtgcc gtcccaatgg cccaacgtgt cgccgccaag cgtttcgccg agcgtctttc
     3841 cacccacttc accgccgccc accaccaacg tggatacgac atcggatccg gagagggacg
     3901 cggatccctt cttacctgcg gagatgttga ggaaaaccca ggaccaatgg agcgcccaca
     3961 accagattct atgccacaag acctttccga ggcccttaag gaggctacca aggaggttca
     4021 cacccaagct gagaacgctg agttcatgcg caacttccaa aagggacaag ttacccgtga
     4081 tggattcaag cttgtcatgg cttccctcta ccacatctac gtcgctcttg aggaggagat
     4141 cgagcgcaac aaggtaagtt taaacatata tatactaact aaccctgatt atttaaattt
     4201 tcaggagtct ccagttttcg ctccagttta cttcccagaa gagctccacc gtaaggctgc
     4261 ccttgagcaa gaccttgcct tctggtatgg accacgctgg caagaagtta tcccatacac
     4321 tccagctatg caacgctacg ttaagcgtct tcacgaggtc ggacgtaccg agccagagct
     4381 tcttgtcgct cacgcttaca cccgttacct tggagacctt tccggaggac aagtccttaa
     4441 ggtaagttta aacagttcgg tactaactaa ccatacatat ttaaattttc agaagatcgc
     4501 tcaaaaggct cttgaccttc catcttccgg agagggactt gccttcttca ctttcccaaa
     4561 catcgcttct gccactaagt tcaagcaact ttaccgctct cgtatgaact ctcttgagat
     4621 gaccccagct gtccgccaac gtgtcatcga ggaggctaag gtaagtttaa acatgatttt
     4681 actaactaac taatctgatt taaattttca gaccgctttc cttcttaaca tccaactttt
     4741 cgaagagctt caagagcttc ttacccacga caccaaggac caatctccat cccgtgcccc
     4801 aggacttcgt caacgtgcct ccaacaaggt ccaagattcc gctccagttg agactccacg
     4861 tggaaagcca ccacttaaca cccgttccca agccccactt cttcgttggg tccttaccct
     4921 ctccttcctt gtcgccaccg tcgctgtcgg actttacgcc atgtaagagc tccgcatcgg
     4981 ccgctgtcat cagatcgcca tctcgcgccc gtgcctctga cttctaagtc caattactct
     5041 tcaacatccc tacatgctct ttctccctgt gctcccaccc cctatttttg ttattatcaa
     5101 aaaacttctc ttaatttctt tgttttttag cttcttttaa gtcacctcta acaatgaaat
     5161 tgtgtagatt caaaaataga attaattcgt aataaaaagt cgaaaaaaat tgtgctccct
     5221 ccccccatta ataataattc tatcccaaaa tctacacaat gttctgtgta cacttcttat
     5281 gttttttact tctgataaat ttttttgaaa catcatagaa aaaaccgcac acaaaatacc
     5341 ttatcatatg ttacgtttca gtttatgacc gcaattttta tttcttcgca cgtctgggcc
     5401 tctcatgacg tcaaatcatg ctcatcgtga aaaagttttg gagtattttt ggaatttttc
     5461 aatcaagtga aagtttatga aattaatttt cctgcttttg ctttttgggg tttcccctat
     5521 tgtttgtcaa gatttcgagg acggcgtttt tcttgctaaa atcacaagta ttgatgagca
     5581 cgatgcaaga aagatcggaa gaaggtttgg gtttgaggct cagtggaagg tgagtagaag
     5641 ttgataattt gaaagtggag tagtgtctat ggggtttttg ccttaaatga cagaatacat
     5701 tcccaatata ccaaacataa ctgtttctac tagtcggccg tacgggccct ttcgtctcgc
     5761 gcgtttcggt gatgacggtg aaaacctctg acacatgcag ctcccggaga cggtcacagc
     5821 ttgtctgtaa gcggatgccg ggagcagaca agcccgtcag ggcgcgtcag cgggtgttgg
     5881 cgggtgtcgg ggctggctta actatgcggc atcagagcag attgtactga gagtgcacca
     5941 tatgcggtgt gaaataccgc acagatgcgt aaggagaaaa taccgcatca ggcggcctta
     6001 attaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct
     6061 tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc
     6121 taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa
     6181 tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt
     6241 gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct
     6301 gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc
     6361 cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta
     6421 tgtggcgcgg tattatcccg tattgacgcc gggcaagagc aactcggtcg ccgcatacac
     6481 tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc
     6541 atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac
     6601 ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg
     6661 gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac
     6721 gagcgtgaca ccacgatgcc tgtagcaatg gcaacaacgt tgcgcaaact attaactggc
     6781 gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt
     6841 gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga
     6901 gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc
     6961 cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag
     7021 atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca
     7081 tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc
     7141 ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca
     7201 gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc
     7261 tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta
     7321 ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgttctt
     7381 ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc
     7441 gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg
     7501 ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg
     7561 tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag
     7621 ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc
     7681 agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat
     7741 agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg
     7801 gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc
     7861 tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt
     7921 accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca
     7981 gtgagcgagg aagcggaaga gcgcccaata cgcaaaccgc ctctccccgc gcgttggccg
     8041 attcattaat gcagctggca cgacaggttt cccgactgga aagcgggcag tgagcgcaac
     8101 gcaattaatg tgagttagct cactcattag gcaccccagg ctttacactt tatgcttccg
     8161 gctcgtatgt tgtgtggaat tgtgagcgga taacaatttc acacaggaaa cagct
//
