LOCUS       sequence-410677-        8358 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Construct for expressing murine progranulin (Grn) in AAV.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8358)
  AUTHORS   Arrant AE, Filiano AJ, Unger DE, Young AH, Roberson ED
  TITLE     Restoring neuronal progranulin reverses deficits in a mouse model of
            frontotemporal dementia.
  JOURNAL   Brain. 2017 May 1;140(5):1447-1465. doi: 10.1093/brain/awx060.
  PUBMED    28379303
REFERENCE   2  (bases 1 to 8358)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10"; journalName: 
            "Brain"; date: "2017-05-1- 1"; volume: "140"; issue: "5"; pages: 
            "1447-1465"
COMMENT     Sequence Label: pAAV-mGrn-myc
FEATURES             Location/Qualifiers
     source          1..8358
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(189..208)
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     enhancer        275..654
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        656..933
                     /label=chicken beta-actin promoter
     primer_bind     1346..1368
                     /label=pCAGGS-5
                     /note="Chimeric intron in CAG promoter, forward primer"
     primer_bind     1430..1449
                     /label=pCAG-F
                     /note="Rabbit beta-globin intron, for pCAG plasmids, 
                     forward primer"
     primer_bind     1527..1546
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        1527..1545
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             3366..3395
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     misc_feature    3441..4029
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(3494..3514)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(3912..3923)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(4121..4140)
                     /label=Bglob-pA-R
                     /note="Rabbit beta-globin polyA region, reverse primer"
     polyA_signal    4186..4241
                     /label=beta-globin poly(A) signal
                     /note="rabbit beta-globin polyadenylation signal (Gil and 
                     Proudfoot, 1987)"
     primer_bind     complement(4240..4259)
                     /label=rbglobpA-R
                     /note="Rabbit beta-globin polyA, reverse primer. Also 
                     called rb-glob-pA-term-R"
     repeat_region   4641..4770
                     /label=AAV2 ITR
     rep_origin      5141..5654
                     /direction=RIGHT
                     /label=M13 ori
                     /note="M13 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(5228..5247)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     5438..5459
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(6136..6155)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     6255..6277
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(6315..6333)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        6401..6505
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(6506..6574,6575..7366)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(6724..6743)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      7537..8125
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     8026..8045
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 cagctgcgcg ctcgctcgct cactgaggcc gcccgggcaa agcccgggcg gcctcagagc
       61 tttgctcgcg cggcctcagt gagcgagcga gcgcgcagag agggagtggc caactccatc
      121 actaggggtt ccttgtagtt aatgattaac ccgccatgct acttatctac gtagccatgc
      181 tctagcggcc tcggcctctg cataaataaa aaaaattagt cagccatgag cttggcccat
      241 tgcatacgtt gtatccatat cataatatgt actcgacatt gattattgac tagttattaa
      301 tagtaatcaa ttacggggtc attagttcat agcccatata tggagttccg cgttacataa
      361 cttacggtaa atggcccgcc tggctgaccg cccaacgacc cccgcccatt gacgtcaata
      421 atgacgtatg ttcccatagt aacgccaata gggactttcc attgacgtca atgggtggag
      481 tatttacggt aaactgccca cttggcagta catcaagtgt atcatatgcc aagtacgccc
      541 cctattgacg tcaatgacgg taaatggccc gcctggcatt atgcccagta catgacctta
      601 tgggactttc ctacttggca gtacatctac gtattagtca tcgctattac catggtcgag
      661 gtgagcccca cgttctgctt cactctcccc atctcccccc cctccccacc cccaattttg
      721 tatttattta ttttttaatt attttgtgca gcgatggggg sggggggggg gggggggcgc
      781 gcgccaggcg gggcggggcg gggcgagggg cggggcgggg cgaggcggag aggtgcggcg
      841 gcagccaatc agagcggcgc gctccgaaag tttcctttta tggcgaggcg gcggcggcgg
      901 cggccctata aaaagcgaag cgcgcggcgg gcgggagtcg ctgcgcgctg ccttcgcccc
      961 gtgccccgct ccgccgccgc ctcgcgccgc ccgccccggc tctgactgac cgcgttactc
     1021 ccacaggtga gcgggcggga cggcccttct cctccgggct gtaattagcg cttggtttaa
     1081 tgacggcttg tttcttttct gtggctgcgt gaaagccttg aggggctccg ggagggccct
     1141 ttgtgcgggg ggagcggctc ggggggtgcg tgcgtgtgtg tgtgcgtggg gagcgccgcg
     1201 tgcggctccg cgctgcccgg cggctgtgag cgctgcgggc gcggcgcggg gctttgtgcg
     1261 ctccgcagtg tgcgcgaggg gagcgcggcc gggggcggtg ccccgcgggg ggacggctgc
     1321 cttcgggggg gacggggcag ggcggggttc ggcttctggc gtgtgaccgg cggctctaga
     1381 gcctctgcta accatgttca tgccttcttc tttttcctac agctcctggg caacgtgctg
     1441 gttattgtgc tgtctcatca ttttggcaaa gaatctcgag ctctctggct aactagagaa
     1501 cccactgctt actggcttat cgaaattaat acgactcact atagggagac ccaagctggc
     1561 tagttaagct tgccgccacc atgtgggtcc tgatgagctg gctggccttc gcggcagggc
     1621 tggtagccgg aacacagtgt ccagatgggc agttctgccc tgttgcctgc tgccttgacc
     1681 agggaggagc caactacagc tgctgtaacc ctcttctgga cacatggcct agaataacga
     1741 gccatcatct agatggctcc tgccagaccc atggccactg tcctgctggc tattcttgtc
     1801 ttctcactgt gtctgggact tccagctgct gcccgttctc taagggtgtg tcttgtggtg
     1861 atggctacca ctgctgcccc cagggcttcc actgtagtgc agatgggaaa tcctgcttcc
     1921 agatgtcaga taaccccttg ggtgctgtcc agtgtcctgg gagccagttt gaatgtcctg
     1981 actctgccac ctgctgcatt atggttgatg gttcgtgggg atgttgtccc atgccccagg
     2041 cctcttgctg tgaagacaga gtgcattgct gtccccatgg ggcctcctgt gacctggttc
     2101 acacacgatg cgtttcaccc acgggcaccc acaccctact aaagaagttc cctgcacaaa
     2161 agaccaacag ggcagtgtct ttgccttttt ctgtcgtgtg ccctgatgct aagacccagt
     2221 gtcccgatga ttctacctgc tgtgagctac ccactgggaa gtatggctgc tgtccaatgc
     2281 ccaatgccat ctgctgttcc gaccacctgc actgctgccc ccaggacact gtatgtgacc
     2341 tgatccagag taagtgccta tccaagaact acaccacgga tctcctgacc aagctgcctg
     2401 gatacccagt gaaggaggtg aagtgcgaca tggaggtgag ctgccctgaa ggatatacct
     2461 gctgccgcct caacactggg gcctggggct gctgtccatt tgccaaggcc gtgtgttgtg
     2521 aggatcacat tcattgctgc ccggcagggt ttcagtgtca cacagagaaa ggaacctgcg
     2581 aaatgggtat cctccaagta ccctggatga agaaggtcat agcccccctc cgcctgccag
     2641 acccacagat cttgaagagt gatacacctt gtgatgactt cactaggtgt cctacaaaca
     2701 atacctgctg caaactcaat tctggggact ggggctgctg tcccatccca gaggctgtct
     2761 gctgctcaga caaccagcat tgctgccctc agggcttcac atgtctggct caggggtact
     2821 gtcagaaggg agacacaatg gtggctggcc tggagaagat acctgcccgc cagacaaccc
     2881 cgctccaaat tggagatatc ggttgtgacc agcataccag ctgcccagta gggcaaacct
     2941 gctgcccaag cctcaaggga agttgggcct gctgccagct gccccatgct gtgtgctgtg
     3001 aggaccggca gcactgttgc ccggccgggt acacctgcaa tgtgaaggcg aggacctgtg
     3061 agaaggatgt cgattttatc cagcctcccg tgctcctgac cctcggccct aaggttggga
     3121 atgtggagtg tggagaaggg catttctgcc atgataacca gacctgttgt aaagacagtg
     3181 caggagtctg ggcctgctgt ccctacctaa agggtgtctg ctgtagagat ggacgtcact
     3241 gttgccccgg tggcttccac tgttcagcca ggggaaccaa gtgtttgcga aagaagattc
     3301 ctcgctggga catgtttttg agggatccgg tcccaagacc gctactggct cgaggtcacc
     3361 cattcgaaca aaaactcatc tcagaagagg atctgaatat gcattaagcg gccgctagcg
     3421 atatcggtac cggatccgat aatcaacctc tggattacaa aatttgtgaa agattgactg
     3481 gtattcttaa ctatgttgct ccttttacgc tatgtggata cgctgcttta atgcctttgt
     3541 atcatgctat tgcttcccgt atggctttca ttttctcctc cttgtataaa tcctggttgc
     3601 tgtctcttta tgaggagttg tggcccgttg tcaggcaacg tggcgtggtg tgcactgtgt
     3661 ttgctgacgc aacccccact ggttggggca ttgccaccac ctgtcagctc ctttccggga
     3721 ctttcgcttt ccccctccct attgccacgg cggaactcat cgccgcctgc cttgcccgct
     3781 gctggacagg ggctcggctg ttgggcactg acaattccgt ggtgttgtcg gggaaatcat
     3841 cgtcctttcc ttggctgctc gcctgtgttg ccacctggat tctgcgcggg acgtccttct
     3901 gctacgtccc ttcggccctc aatccagcgg accttccttc ccgcggcctg ctgccggctc
     3961 tgcggcctct tccgcgtctt cgccttcgcc ctcagacgag tcggatctcc ctttgggccg
     4021 cctccccgca tcggatccaa ttcactcctc aggtgcaggc tgcctatcag aaggtggtgg
     4081 ctggtgtggc caatgccctg gctcacaaat accactgaga tctttttccc tctgccaaaa
     4141 attatgggga catcatgaag ccccttgagc atctgacttc tggctaataa aggaaattta
     4201 ttttcattgc aatagtgtgt tggaattttt tgtgtctctc actcggaagg acatatggga
     4261 gggcaaatca tttaaaacat cagaatgagt atttggttta gagtttggca acatatgccc
     4321 atatgctggc tgccatgaac aaaggttggc tataaagagg tcatcagtat atgaaacagc
     4381 cccctgctgt ccattcctta ttccatagaa aagccttgac ttgaggttag atttttttta
     4441 tattttgttt tgtgttattt ttttctttaa catccctaaa attttcctta catgttttac
     4501 tagccagatt tttcctcctc tcctgactac tcccagtcat agctgtccct cttctcttat
     4561 ggagatccct cgacctgcag cccaagctct agagcatggc tacgtagata agtagcatgg
     4621 cgggttaatc attaactaca aggaacccct agtgatggag ttggccactc cctctctgcg
     4681 cgctcgctcg ctcactgagg ccgggcgacc aaaggtcgcc cgacgcccgg gctttgcccg
     4741 ggcggcctca gtgagcgagc gagcgcgcag ctggcgtaat agcgaagagg cccgcaccga
     4801 tcgcccttcc caacagttgc gcagcctgaa tggcgaatgg cgattccgtt gcaatggctg
     4861 gcggtaatat tgttctggat attaccagca aggccgatag tttgagttct tctactcagg
     4921 caagtgatgt tattactaat caaagaagta ttgcgacaac ggttaatttg cgtgatggac
     4981 agactctttt actcggtggc ctcactgatt ataaaaacac ttctcaggat tctggcgtac
     5041 cgttcctgtc taaaatccct ttaatcggcc tcctgtttag ctcccgctct gattctaacg
     5101 aggaaagcac gttatacgtg ctcgtcaaag caaccatagt acgcgccctg tagcggcgca
     5161 ttaagcgcgg cgggtgtggt ggttacgcgc agcgtgaccg ctacacttgc cagcgcccta
     5221 gcgcccgctc ctttcgcttt cttcccttcc tttctcgcca cgttcgccgg ctttccccgt
     5281 caagctctaa atcgggggct ccctttaggg ttccgattta gtgctttacg gcacctcgac
     5341 cccaaaaaac ttgattaggg tgatggttca cgtagtgggc catcgccctg atagacggtt
     5401 tttcgccctt tgacgttgga gtccacgttc tttaatagtg gactcttgtt ccaaactgga
     5461 acaacactca accctatctc ggtctattct tttgatttat aagggatttt gccgatttcg
     5521 gcctattggt taaaaaatga gctgatttaa caaaaattta acgcgaattt taacaaaata
     5581 ttaacgctta caatttaaat atttgcttat acaatcttcc tgtttttggg gcttttctga
     5641 ttatcaaccg gggtacatat gattgacatg ctagttttac gattaccgtt catcgattct
     5701 cttgtttgct ccagactctc aggcaatgac ctgatagcct ttgtagagac ctctcaaaaa
     5761 tagctaccct ctccggcatg aatttatcag ctagaacggt tgaatatcat attgatggtg
     5821 atttgactgt ctccggcctt tctcacccgt ttgaatcttt acctacacat tactcaggca
     5881 ttgcatttaa aatatatgag ggttctaaaa atttttatcc ttgcgttgaa ataaaggctt
     5941 ctcccgcaaa agtattacag ggtcataatg tttttggtac aaccgattta gctttatgct
     6001 ctgaggcttt attgcttaat tttgctaatt ctttgccttg cctgtatgat ttattggatg
     6061 ttggaatcgc ctgatgcggt attttctcct tacgcatctg tgcggtattt cacaccgcat
     6121 atggtgcact ctcagtacaa tctgctctga tgccgcatag ttaagccagc cccgacaccc
     6181 gccaacaccc gctgacgcgc cctgacgggc ttgtctgctc ccggcatccg cttacagaca
     6241 agctgtgacc gtctccggga gctgcatgtg tcagaggttt tcaccgtcat caccgaaacg
     6301 cgcgagacga aagggcctcg tgatacgcct atttttatag gttaatgtca tgataataat
     6361 ggtttcttag acgtcaggtg gcacttttcg gggaaatgtg cgcggaaccc ctatttgttt
     6421 atttttctaa atacattcaa atatgtatcc gctcatgaga caataaccct gataaatgct
     6481 tcaataatat tgaaaaagga agagtatgag tattcaacat ttccgtgtcg cccttattcc
     6541 cttttttgcg gcattttgcc ttcctgtttt tgctcaccca gaaacgctgg tgaaagtaaa
     6601 agatgctgaa gatcagttgg gtgcacgagt gggttacatc gaactggatc tcaacagcgg
     6661 taagatcctt gagagttttc gccccgaaga acgttttcca atgatgagca cttttaaagt
     6721 tctgctatgt ggcgcggtat tatcccgtat tgacgccggg caagagcaac tcggtcgccg
     6781 catacactat tctcagaatg acttggttga gtactcacca gtcacagaaa agcatcttac
     6841 ggatggcatg acagtaagag aattatgcag tgctgccata accatgagtg ataacactgc
     6901 ggccaactta cttctgacaa cgatcggagg accgaaggag ctaaccgctt ttttgcacaa
     6961 catgggggat catgtaactc gccttgatcg ttgggaaccg gagctgaatg aagccatacc
     7021 aaacgacgag cgtgacacca cgatgcctgt agcaatggca acaacgttgc gcaaactatt
     7081 aactggcgaa ctacttactc tagcttcccg gcaacaatta atagactgga tggaggcgga
     7141 taaagttgca ggaccacttc tgcgctcggc ccttccggct ggctggttta ttgctgataa
     7201 atctggagcc ggtgagcgtg ggtctcgcgg tatcattgca gcactggggc cagatggtaa
     7261 gccctcccgt atcgtagtta tctacacgac ggggagtcag gcaactatgg atgaacgaaa
     7321 tagacagatc gctgagatag gtgcctcact gattaagcat tggtaactgt cagaccaagt
     7381 ttactcatat atactttaga ttgatttaaa acttcatttt taatttaaaa ggatctaggt
     7441 gaagatcctt tttgataatc tcatgaccaa aatcccttaa cgtgagtttt cgttccactg
     7501 agcgtcagac cccgtagaaa agatcaaagg atcttcttga gatccttttt ttctgcgcgt
     7561 aatctgctgc ttgcaaacaa aaaaaccacc gctaccagcg gtggtttgtt tgccggatca
     7621 agagctacca actctttttc cgaaggtaac tggcttcagc agagcgcaga taccaaatac
     7681 tgttcttcta gtgtagccgt agttaggcca ccacttcaag aactctgtag caccgcctac
     7741 atacctcgct ctgctaatcc tgttaccagt ggctgctgcc agtggcgata agtcgtgtct
     7801 taccgggttg gactcaagac gatagttacc ggataaggcg cagcggtcgg gctgaacggg
     7861 gggttcgtgc acacagccca gcttggagcg aacgacctac accgaactga gatacctaca
     7921 gcgtgagcta tgagaaagcg ccacgcttcc cgaagggaga aaggcggaca ggtatccggt
     7981 aagcggcagg gtcggaacag gagagcgcac gagggagctt ccagggggaa acgcctggta
     8041 tctttatagt cctgtcgggt ttcgccacct ctgacttgag cgtcgatttt tgtgatgctc
     8101 gtcagggggg cggagcctat ggaaaaacgc cagcaacgcg gcctttttac ggttcctggc
     8161 cttttgctgg ccttttgctc acatgttctt tcctgcgtta tcccctgatt ctgtggataa
     8221 ccgtattacc gcctttgagt gagctgatac cgctcgccgc agccgaacga ccgagcgcag
     8281 cgagtcagtg agcgaggaag cggaagagcg cccaatacgc aaaccgcctc tccccgcgcg
     8341 ttggccgatt cattaatg
//
