LOCUS       sequence-410702-       10361 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Knock-in vector to insert a dox-inducible PAX7 transgene into the 
            CYBL locus in human pluripotent stem cells. PAX7 induction results 
            in myogenic differentiation..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10361)
  AUTHORS   Harley P, Kerins C, Gatt A, Neves G, Riccio F, Machado CB, 
            Cheesbrough A, R'Bibo L, Burrone J, Lieberam I
  TITLE     Aberrant axon initial segment plasticity and intrinsic excitability 
            of ALS hiPSC motor neurons.
  JOURNAL   Cell Rep. 2023 Dec 26;42(12):113509. doi: 
            10.1016/j.celrep.2023.113509. Epub 2023 Nov 28.
  PUBMED    38019651
REFERENCE   2  (bases 1 to 10361)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell Rep.";
            date: "2023-12-26"; volume: "42(12):113509. doi"; pages: " 
            10.1016/j.celrep.2023.113509. Epub 2023 Nov 28"
COMMENT     Sequence Label: CLYBL-TO-PAX7
FEATURES             Location/Qualifiers
     source          1..10361
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     266..285
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     protein_bind    1391..1409
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    1427..1445
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    1463..1481
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    1499..1517
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    1535..1553
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    1571..1589
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    1607..1625
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     primer_bind     1670..1694
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     regulatory      1777..1786
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     regulatory      1781..1790
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     polyA_signal    3358..3479
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(3395..3414)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     3449..3468
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(3528..3544)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(3528..3544)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(3541..3563)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    3552..3568
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(3576..3582,3583..3600,3601..3606))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    3621..3642
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(3759..3776)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     primer_bind     4068..4087
                     /label=rbglobpA-R
                     /note="Rabbit beta-globin polyA, reverse primer. Also 
                     called rb-glob-pA-term-R"
     polyA_signal    4086..4141
                     /label=beta-globin poly(A) signal
                     /note="rabbit beta-globin polyadenylation signal (Gil and 
                     Proudfoot, 1987)"
     primer_bind     4187..4206
                     /label=Bglob-pA-R
                     /note="Rabbit beta-globin polyA region, reverse primer"
     CDS             complement(4297..5043)
                     /codon_start=1
                     /product="modified rtTA protein that binds tightly to 
                     promoters containing the tet operator in the presence of 
                     doxycycline"
                     /label=Tet-On(R) 3G
                     /translation="MSRLDKSKVINSALELLNGVGIEGLTTRKLAQKLGVEQPTLYWHV
                     KNKRALLDALPIEMLDRHHTHSCPLEGESWQDFLRNNAKSYRCALLSHRDGAKVHLGTR
                     PTEKQYETLENQLAFLCQQGFSLENALYALSAVGHFTLGCVLEEQEHQVAKEERETPTT
                     DSMPPLLKQAIELFDRQGAEPAFLFGLELIICGLEKQLKCESGGPTDALDDFDLDMLPA
                     DALDDFDLDMLPADALDDFDLDMLPG"
     primer_bind     complement(5075..5094)
                     /label=pCAG-F
                     /note="Rabbit beta-globin intron, for pCAG plasmids, 
                     forward primer"
     intron          5102..6118
                     /label=chimeric intron
                     /note="chimera between introns from chicken beta-actin and 
                     rabbit beta-globin"
     primer_bind     complement(5156..5178)
                     /label=pCAGGS-5
                     /note="Chimeric intron in CAG promoter, forward primer"
     promoter        complement(6119..6400)
                     /label=chicken beta-actin promoter
     enhancer        6402..6781
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     polyA_signal    6787..7011
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     primer_bind     7000..7017
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     protein_bind    7033..7066
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     polyA_signal    7089..7313
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     CDS             complement(7318..8118)
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="GPRIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQG
                     RPVLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDL
                     LSSHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEE
                     HQGLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQD
                     IALATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(7429..7448)
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     primer_bind     8039..8058
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     CDS             complement(8113..8166)
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
     misc_feature    8190..8215
                     /label=SA
                     /note="splice acceptor site"
     protein_bind    8216..8249
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     CDS             join(9107..9175,9176..9967)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(9325..9344)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      join(10138..10361,1..365)
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
ORIGIN
        1 tgttaccagt ggctgctgcc agtggcgata agtcgtgtct taccgggttg gactcaagac
       61 gatagttacc ggataaggcg cagcggtcgg gctgaacggg gggttcgtgc acacagccca
      121 gcttggagcg aacgacctac accgaactga gatacctaca gcgtgagcta tgagaaagcg
      181 ccacgcttcc cgaagggaga aaggcggaca ggtatccggt aagcggcagg gtcggaacag
      241 gagagcgcac gagggagctt ccagggggaa acgcctggta tctttatagt cctgtcgggt
      301 ttcgccacct ctgacttgag cgtcgatttt tgtgatgctc gtcagggggg cggagcctat
      361 ggaaaaacgc cagcaacgcg gcctttttac ggttcctggc gaattcgccc ttagagcaga
      421 gccaggaacc cctgtaggga aggggcagga gagccagggg catgagatgg tggacgagga
      481 agggggacag ggaagcctga gcgcctctcc tgggcttgcc aaggactcaa acccagaagc
      541 ccagagcagg gccttaggga agcgggacgg ctctgttgga gagtccagta ttgaattagt
      601 gatagataca cagaaaaaaa gcaaattgaa taggtgaatg gataaacaaa ctgtggaaca
      661 tccagacaat gaaatattca ccaataaaaa gaaatgagct atcaagccac attggctatg
      721 cccttcacat ttaaggagga accttaaatg catattgcca aattttaaaa gctagtctga
      781 aaatgctaca gactgtatga ttccaattat atgacatttt agaaaaggca aaactataga
      841 gatcagtggt tgccagcggc tcatggaggc aggatgaatt ggtggaacac agggatgctt
      901 tcagggtaat gaaaatatct tgtgtgatcc tgtaataatg gatacgtggc ataatgcatt
      961 tgttaaaacc tgtaaactag ccaggtatag tggctttttt gcacctatag tctcagctac
     1021 ttggtaggct gagacaggaa gatcacttga gcccaggact tcaagaccag cctgggtaat
     1081 atagtaagac cccatctcta ttttttaaag aaattaagaa agaaaagaaa cccatcaact
     1141 gtataacaca aagaatcgta atgtaaacta cagtcctgag taatgaaaca tttttaaaga
     1201 aataaataaa ttcaaggcaa ttattaactt caggaaaaac agtaaagtaa acataagtat
     1261 acaaatcaaa catggctcag ttgtgaaaat atttacaagg tcactttaag taacattgaa
     1321 tgttgatata atcccaaaat atatttatgt tggaaggatg aggagatatc tccaccccac
     1381 agtggtttac tccctatcag tgatagagaa cgtatgaaga gtttactccc tatcagtgat
     1441 agagaacgta tgcagacttt actccctatc agtgatagag aacgtataag gagtttactc
     1501 cctatcagtg atagagaacg tatgaccagt ttactcccta tcagtgatag agaacgtatc
     1561 tacagtttac tccctatcag tgatagagaa cgtatatcca gtttactccc tatcagtgat
     1621 agagaacgta taagctttag gcgtgtacgg tgggcgccta taaaagcaga gctcgtttag
     1681 tgaaccgtca gatcgcctgg agcaattcca caacactttt gtcttatacc aactttccgt
     1741 accacttcct accctcgtaa acttaaggtt aattaagcca ccatggcggc ccttcccggc
     1801 acggtaccga gaatgatgcg gccggctccg gggcagaact acccccgcac gggattccct
     1861 ttggaagtgt ccaccccgct tggccaaggc cgggtcaatc agctgggagg ggtcttcatc
     1921 aatgggcgac ccctgcctaa ccacatccgc cacaagatag tggagatggc ccaccatggc
     1981 atccggccct gtgtcatctc ccgacagctg cgtgtctccc acggctgcgt ctccaagatt
     2041 ctttgccgct accaggagac cgggtccatc cggcctgggg ccatcggcgg cagcaagccc
     2101 agacaggtgg cgactccgga tgtagagaaa aagattgagg agtacaagag ggaaaaccca
     2161 ggcatgttca gctgggagat ccgggacagg ctgctgaagg atgggcactg tgaccgaagc
     2221 actgtgccct cagtgagttc gattagccgc gtgctcagaa tcaagttcgg gaagaaagag
     2281 gaggaggatg aagcggacaa gaaggaggac gacggcgaaa agaaggccaa acacagcatc
     2341 gacggcatcc tgggcgacaa agggaaccgg ctggacgagg gctcggatgt ggagtcggaa
     2401 cctgacctcc cactgaagcg caagcagcga cgcagtcgga ccacattcac ggccgagcag
     2461 ctggaggagc tggagaaggc ctttgagagg acccactacc cagacatata cacccgcgag
     2521 gagctggcgc agaggaccaa gctgacagag gcgcgtgtgc aggtctggtt cagtaaccgc
     2581 cgcgcccgtt ggcgtaagca ggcaggagcc aaccagctgg cggcgttcaa ccaccttctg
     2641 ccaggaggct tcccgcccac cggcatgccc acgctgcccc cctaccagct gccggactcc
     2701 acctacccca ccaccaccat ctcccaagat gggggcagca ctgtgcaccg gcctcagccc
     2761 ctgccaccgt ccaccatgca ccagggcggg ctggctgcag cggctgcagc cgccgacacc
     2821 agctctgcct acggagcccg ccacagcttc tccagctact ctgacagctt catgaatccg
     2881 gcggcgccct ccaaccacat gaacccggtc agcaacggcc tgtctcctca ggtgatgagc
     2941 atcttgggca accccagtgc ggtgcccccg cagccacagg ctgacttctc catctccccg
     3001 ctgcatggcg gcctggactc ggccacctcc atctcagcca gctgcagcca gcgggccgac
     3061 tccatcaagc caggagacag cctgcccacc tcccaggcct actgcccacc cacctacagc
     3121 accaccggct acagcgtgga ccccgtggcc ggctatcagt acggccagta cggccagagt
     3181 gagtgcctgg tgccctgggc gtcccccgtc cccattcctt ctcccacccc cagggcctcc
     3241 tgcttgttta tggagagcta caaggtggtg tcagggtggg gaatgtccat ttcacagatg
     3301 gaaaaattga agtccagcca gatggaacag ttcacctaat tcgaaatcgc ccaccccaac
     3361 ttgtttattg cagcttataa tggttacaaa taaagcaata gcatcacaaa tttcacaaat
     3421 aaagcatttt tttcactgca ttctagttgt ggtttgtcca aactcatcaa tgtatcttat
     3481 catgtctgta taccgtcgac ctctagctag agcttggcgt aatcatggtc atagctgttt
     3541 cctgtgtgaa attgttatcc gctcacaatt ccacacaaca tacgagccgg aagcataaag
     3601 tgtaaagcct ggggtgccta atgagtgagc taactcacat taattgcgtt gcgctcactg
     3661 cccgctttcc agtcgggaaa cctgtcgtgc cagctgcatt aatgaatcgg ccaacgcgcg
     3721 gggagaggcg gtttgcgtat tgggcgctct tccgcttcct cgctcactga ctcgctgcgc
     3781 tcggtcgttc ggctgcggcg agcggtatca gctcactcaa aggacggccg cacatgtaag
     3841 gaaaatttta gggatgttaa agaaaaaaat aacacaaaac aaaatataaa aaaaatctaa
     3901 cctcaagtca aggcttttct atggaataag gaatggacag cagggggctg tttcatatac
     3961 tgatgacctc tttatagcca acctttgttc atggcagcca gcatatgggc atatgttgcc
     4021 aaactctaaa ccaaatactc attctgatgt tttaaatgat ttgccctccc atatgtcctt
     4081 ccgagtgaga gacacaaaaa attccaacac actattgcaa tgaaaataaa tttcctttat
     4141 tagccagaag tcagatgctc aaggggcttc atgatgtccc cataattttt ggcagaggga
     4201 aaaagatctc agtggtattt gtgagccagg gcattggcca caccagtcac caccttctga
     4261 taggcagcct gcacctgagg agtgaattca cgcgttttag cctggcagca tatcgagatc
     4321 gaagtcatcg agagcgtcag cagggagcat atccagatca aaatcatcca gggcatcggc
     4381 gggcagcatg tccaggtcga agtcgtccag ggcgtcggtg ggtccgccgc tctcgcactt
     4441 cagctgcttc tcgaggccgc agatgatcag ttccaggccg aacaggaagg cgggctcggc
     4501 gccctgtctg tcgaacagct cgatggcctg cttcagcagg gggggcatgc tgtcggtggt
     4561 gggtgtctct ctttcctctt tggcgacctg gtgttcctgt tcttccagca cgcagcccag
     4621 ggtgaagtgg cccacggcgc tcagggcgta cagggcgttt tccaggctga agccctgctg
     4681 gcacaggaag gccagctggt tttccagtgt ctcgtactgc ttctcggtgg gtctggtgcc
     4741 caggtgcact ttggcgccgt cccggtggct cagcagggcg catctgtagc tcttggcgtt
     4801 gttccgcagg aagtcctgcc agctctcgcc ttccagaggg cagctgtggg tgtggtgccg
     4861 gtccagcatc tcgatgggca gggcgtccag cagggcccgc ttgttcttca cgtgccagta
     4921 cagggtgggc tgttccacgc ccagtttctg ggccagcttc cgggtggtca ggccctcgat
     4981 gcccacgccg ttcagcagtt ccagggcgct gttgatcact ttgctcttgt ccagccggct
     5041 catgtcgaca ttctttgcca aaatgatgag acagcacaat aaccagcacg ttgcccagga
     5101 gctgtaggaa aaagaagaag gcatgaacat ggttagcaga ggctctagag ccgccggtca
     5161 cacgccagaa gccgaacccc gccctgcccc gtcccccccg aaggcagccg tccccccgcg
     5221 gacagccccg aggctggaga gggagaaggg gacggcggcg cggcgacgca cgaaggccct
     5281 ccccgcccat ttccttcctg ccggcgccgc accgcttcgc cccgcgcccg ctagaggggg
     5341 tgcggcggcg cctcccagat ttcggctccg cacagatttg ggacaaagga agtccctgcg
     5401 ccctctcgca cgattaccat aaaaggcaat ggctgcggct cgccgcgcct cgacagccgc
     5461 cggcgctccg ggggccgccg cgcccctccc ccgagccctc cccggcccga ggcggccccg
     5521 ccccgcccgg cacccccacc tgccgccacc ccccgcccgg cacggcgagc cccgcgccac
     5581 gccccgtacg gagccccgca cccgaagccg ggccgtgctc agcaactcgg ggaggggggt
     5641 gcaggggggg ttgcagcccg accgacgcgc ccacaccccc tgctcacccc cccacgcaca
     5701 caccccgcac gcagcctttg ttcccctcgc agcccccccc gcaccgcggg gcaccgcccc
     5761 cggccgcgct cccctcgcgc acactgcgga gcgcacaaag ccccgcgccg cgcccgcagc
     5821 gctcacagcc gccgggcagc gcggagccgc acgcggcgct ccccacgcac acacacacgc
     5881 acgcaccccc cgagccgctc cccccgcaca aagggccctc ccggagcccc tcaaggcttt
     5941 cacgcagcca cagaaaagaa acaagccgtc attaaaccaa gcgctaatta cagcccggag
     6001 gagaagggcc gtcccgcccg ctcacctgtg ggagtaacgc ggtcagtcag agccggggcg
     6061 ggcggcgcga ggcggcggcg gagcggggca cggggcgaag gcagcgcgca gcgactcccg
     6121 cccgccgcgc gcttcgcttt ttatagggcc gccgccgccg ccgcctcgcc ataaaaggaa
     6181 actttcggag cgcgccgctc tgattggctg ccgccgcacc tctccgcctc gccccgcccc
     6241 gcccctcgcc ccsccccscc ccsccysgcg cgcgcccccc cccccccccc ccccgccccc
     6301 atcgctgcac aaaataatta aaaaataaat aaatacaaaa ttgggggtgg ggaggggggg
     6361 gagatgggga gagtgaagca gaacgtgggg ctcacctcga ccatggtaat agcgatgact
     6421 aatacgtaga tgtactgcca agtaggaaag tcccataagg tcatgtactg ggcataatgc
     6481 caggcgggcc atttaccgtc attgacgtca atagggggcg tacttggcat atgatacact
     6541 tgatgtactg ccaagtgggc agtttaccgt aaatactcca cccattgacg tcaatggaaa
     6601 gtccctattg gcgttactat gggaacatac gtcattattg acgtcaatgg gcgggggtcg
     6661 ttgggcggtc agccaggcgg gccatttacc gtaagttatg taacgcggaa ctccatatat
     6721 gggctatgaa ctaatgaccc cgtaattgat tactattaat aactagtcaa taatcaatgt
     6781 cgagacccat agagcccacc gcatccccag catgcctgct attgtcttcc caatcctccc
     6841 ccttgctgtc ctgccccacc ccacccccca gaatagaatg acacctactc agacaatgcg
     6901 atgcaatttc ctcattttat taggaaagga cagtgggagt ggcaccttcc agggtcaagg
     6961 aaggcacggg ggaggggcaa acaacagatg gctggcaact agaaggcaca gtcgaggctg
     7021 atcagcgggt ttataacttc gtataatgta tgctatacga agttatggta ccatcgcaga
     7081 tcatgcatcc atagagccca ccgcatcccc agcatgcctg ctattgtctt cccaatcctc
     7141 ccccttgctg tcctgcccca ccccaccccc cagaatagaa tgacacctac tcagacaatg
     7201 cgatgcaatt tcctcatttt attaggaaag gacagtggga gtggcacctt ccagggtcaa
     7261 ggaaggcacg ggggaggggc aaacaacaga tggctggcaa ctagaaggca caggttttta
     7321 gaagaactcg tcaagaaggc gatagaaggc gatgcgctgc gaatcgggag cggcgatacc
     7381 gtaaagcacg aggaagcggt cagcccattc gccgccaagc tcttcagcaa tatcacgggt
     7441 agccaacgct atgtcctgat agcggtccgc cacacccagc cggccacagt cgatgaatcc
     7501 agaaaagcgg ccattttcca ccatgatatt cggcaagcag gcatcgccat gggtcacgac
     7561 gagatcctcg ccgtcgggca tgcgcgcctt gagcctggcg aacagttcgg ctggcgcgag
     7621 cccctgatgc tcttcgtcca gatcatcctg atcgacaaga ccggcttcca tccgagtacg
     7681 tgctcgctcg atgcgatgtt tcgcttggtg gtcgaatggg caggtagccg gatcaagcgt
     7741 atgcagccgc cgcattgcat cagccatgat ggatactttc tcggcaggag caaggtgaga
     7801 tgacaggaga tcctgccccg gcacttcgcc caatagcagc cagtcccttc ccgcttcagt
     7861 gacaacgtcg agcacagctg cgcaaggaac gcccgtcgtg gccagccacg atagccgcgc
     7921 tgcctcgtcc tgcagttcat tcagggcacc ggacaggtcg gtcttgacaa aaagaaccgg
     7981 gcgcccctgc gctgacagcc ggaacacggc ggcatcagag cagccgattg tctgttgtgc
     8041 ccagtcatag ccgaatagcc tctccaccca agcggccgga gaacctgcgt gcaatccatc
     8101 ttgttcaatc ctagggccgg gattctcctc cacgtcaccg catgttagaa gacttcctct
     8161 gccctctccg ctgccagatc tctcgaggcc ctgtgggagg aagagaagag gtcagataac
     8221 ttcgtataat gtatgctata cgaagttatg cggccgctgg gagaagggta agagagctca
     8281 atcctcatct tacatagaag gaagtcagta gataatatct tacatttaaa agtcaagaaa
     8341 taacagtctg accatactat ctagaaatat ggaagttaac agtaaggaag ttaaaacagt
     8401 tgaaagtagt ttcgtctaga aagaaagact tggggaagaa caaagaaggc taattttcat
     8461 tatgagcttt attgtaacag aaaagagacc actaactcag ctaggttttc ccaattcctc
     8521 aacactttgg ggcacagtgt ttagtcagag ctgtgatcac tctctgagca catggattgt
     8581 atcttaattt tcatctcatt gaaaatatgt accatttgtc caagtggaaa ctgggctgaa
     8641 gcatgactgt gaccaccacc aaagaaatgg ctagactcgc taaggtctgc tggggtgacc
     8701 cgtgattgtg ttgtgcttga tcttgttgag catggctctt ttaagtcacc atacctgccc
     8761 ctgccaatgc accaaacacc aacttaaagg gactttcagc ttttgtaagt cagtctcagc
     8821 cttacttctg aaccatttct ttgacttctt tcatgactct gatcctgtcc cagttttaag
     8881 cctcccagcc accagtcatg ctgttttgct ctgctattta cattcttctc tacctacttt
     8941 ggacttgatg gtatgcctac tatattagtt atttattgct gtgtaacaaa ccactctgaa
     9001 acatagtggc ttacaaccac aaccatttta ttttattatg aaactgtggg tcagaatgta
     9061 tattggccca ttggcatgat tctcctgcta ttgaaaaagg aagagtatga gtattcaaca
     9121 tttccgtgtc gcccttattc ccttttttgc ggcattttgc cttcctgttt ttgctcaccc
     9181 agaaacgctg gtgaaagtaa aagatgctga agatcagttg ggtgcacgag tgggttacat
     9241 cgaactggat ctcaacagcg gtaagatcct tgagagtttt cgccccgaag aacgttttcc
     9301 aatgatgagc acttttaaag ttctgctatg tggcgcggta ttatcccgta ttgacgccgg
     9361 gcaagagcaa ctcggtcgcc gcatacacta ttctcagaat gacttggttg agtactcacc
     9421 agtcacagaa aagcatctta cggatggcat gacagtaaga gaattatgca gtgctgccat
     9481 aaccatgagt gataacactg cggccaactt acttctgaca acgatcggag gaccgaagga
     9541 gctaaccgct tttttgcaca acatggggga tcatgtaact cgccttgatc gttgggaacc
     9601 ggagctgaat gaagccatac caaacgacga gcgtgacacc acgatgcctg tagcaatggc
     9661 aacaacgttg cgcaaactat taactggcga actacttact ctagcttccc ggcaacaatt
     9721 aatagactgg atggaggcgg ataaagttgc aggaccactt ctgcgctcgg cccttccggc
     9781 tggctggttt attgctgata aatctggagc cggtgagcgt gggtctcgcg gtatcattgc
     9841 agcactgggg ccagatggta agccctcccg tatcgtagtt atctacacga cggggagtca
     9901 ggcaactatg gatgaacgaa atagacagat cgctgagata ggtgcctcac tgattaagca
     9961 ttggtaactg tcagaccaag tttactcata tatactttag attgatttaa aacttcattt
    10021 ttaatttaaa aggatctagg tgaagatcct ttttgataat ctcatgacca aaatccctta
    10081 acgtgagttt tcgttccact gagcgtcaga ccccgtagaa aagatcaaag gatcttcttg
    10141 agatcctttt tttctgcgcg taatctgctg cttgcaaaca aaaaaaccac cgctaccagc
    10201 ggtggtttgt ttgccggatc aagagctacc aactcttttt ccgaaggtaa ctggcttcag
    10261 cagagcgcag ataccaaata ctgttcttct agtgtagccg tagttaggcc accacttcaa
    10321 gaactctgta gcaccgccta catacctcgc tctgctaatc c
//
