LOCUS       sequence-410704-        8629 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Pan-neuronal expression of SNG-1::CRY2olig(535) in neurons of C. 
            elegans.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8629)
  AUTHORS   Vettkotter D, Schneider M, Goulden BD, Dill H, Liewald J, Zeiler S, 
            Guldan J, Ates YA, Watanabe S, Gottschalk A
  TITLE     Rapid and reversible optogenetic silencing of synaptic transmission 
            by clustering of synaptic vesicles.
  JOURNAL   Nat Commun. 2022 Dec 19;13(1):7827. doi: 10.1038/s41467-022-35324-z.
  PUBMED    36535932
REFERENCE   2  (bases 1 to 8629)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2022-12-19"; volume: "13(1):7827. doi"; pages: " 
            10.1038/s41467-022-35324-z"
COMMENT     Sequence Label: pDV12  [psng-1::SNG-1::CRY2olig(535)]
FEATURES             Location/Qualifiers
     source          1..8629
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             3503..4996
                     /codon_start=1
                     /product="N-terminal photolyase homology region of 
                     Arabidopsis thaliana cryptochrome 2"
                     /label=CRY2PHR
                     /note="binds to CIB1 upon exposure to blue light (Kennedy 
                     et al., 2010)"
                     /translation="MKMDKKTIVWFRRDLRIEDNPALAAAAHEGSVFPVFIWCPEEEGQ
                     FYPGRASRWWMKQSLAHLSQSLKALGSDLTLIKTHNTISAILDCIRVTGATKVVFNHLY
                     DPVSLVRDHTVKEKLVERGISVQSYNGDLLYEPWEIYCEKGKPFTSFNSYWKKCLDMSI
                     ESVMLPPPWRLMPITAAAEAIWACSIEELGLENEAEKPSNALLTRAWSPGWSNADKLLN
                     EFIEKQLIDYAKNSKKVVGNSTSLLSPYLHFGEISVRHVFQCARMKQIIWARDKNSEGE
                     ESADLFLRGIGLREYSRYICFNFPFTHEQSLLSHLRFFPWDADVDKFKAWRQGRTGYPL
                     VDAGMRELWATGWMHNRIRVIVSSFAVKFLLLPWKWGMKYFWDTLLDADLECDILGWQY
                     ISGSIPDGHELDRLDNPALQGAKYDPEGEYIRQWLPELARLPTEWIHHPWDAPLTVLKA
                     SGVELGTNYAKPIVDIDTARELLAKAISRTRGAQIMIGAA"
     primer_bind     complement(6057..6079)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     6179..6198
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     complement(6276..6294)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        6362..6466
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(6467..6535,6536..7327)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(6685..6704)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      7498..8086
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     7987..8006
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     8240..8257
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    8374..8395
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(8410..8415,8416..8433,8434..8440)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    8448..8464
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     8453..8475
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     8472..8488
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     8472..8488
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
ORIGIN
        1 ctgcagagag cgtgttctgt ttagggaaat gcatgccctg tacatcatct tccgttgaat
       61 tttaattact tctactaaac atttttttgc ttgacttttt ttgcaaagac taatattttt
      121 tctgaataaa taagtctgtt gtttcctgca ctattctggc atatttcctt ccttttaatt
      181 agtgaagtag ttcctttttt catctgatgc ttgttttttc aagatacgtc atgttctaac
      241 tgaaaacaaa cattttcttt agattcaatt tttctttaga ctcaatttta ggttgacgtt
      301 taggttttaa aaaaatttca ttcaggtttg tttcaaatgt ttaaaaactt ttattttcca
      361 ctgaatgaaa actattaata ttaagcagta cacaaaatga aaggtggatt agtgaagttt
      421 ttttttcttt tttgaagaaa aggcttcata tatgtttaaa ttgtcaaaaa gtaatatcga
      481 aaactgaaag ggtttggatc gttttgagtt gtttgcagca gtacaatttt ttacaattac
      541 acatttaatg caggcaccat aatgttttca aattgcttag caggtcagta ttctatgcaa
      601 ttgtttataa aaagttatat ttttcatgat tgattctctg tttccagctc agttactgat
      661 caattcaata gacgtaattt aataacaaat atgccgcgtt tttcaatttt tcacataagc
      721 gttcttgttc ttgaccaaat catttctctc cgaaaccgca aaatttccca tatctgcgcc
      781 ggttattgaa tattcattgg tttgagctca cttttcatcg ttccatagtc ggtttcctca
      841 ttcaattttc attaataatt cagtatgcat tcactaaatt tgtctccacg gtgatttaat
      901 gaacaagtct ttaatgaatt taatgctgcc tgcggacaag ctagatttag atgttctaat
      961 gaatcatctt tttagaattc atatgttatt aatgaaactt ccttttatcc ctctcttttc
     1021 attgcaccag agactttcaa cttccggaag aaagaattcg ttgaaaagtg cttccaaatc
     1081 gactggcttc ttttactaca ggaggaccct ttgctctgct tttcgccgtc tgtcgttttt
     1141 ttttacgctt tcccttcatg gtcgcactgg gaggggagcc ttctgcgcaa agttgtgggc
     1201 atgaagagtg gctccgtcct tcgtcttctt tttccccgct acttcttcat cttcgctttc
     1261 ggcgtcgtct tctccttctc ggcgagcaga gagtgcctct atctttcttt tcttcactgg
     1321 atgtgtatat ggcgtttaga agtagaagca ttcgtgggac gcactgaatg tcttggaaga
     1381 aagaaaggag aaaagaagct caagattaca cgctttcgtt cttttcgttc ttttcatttc
     1441 atcttttctt ttggtccttc cgtttcaata ttttgtattt acaatgttct tcatttcatt
     1501 ccagctcatt ccatttaata cttagtatgt ctaacagaat ctgaacacct ctgaccccag
     1561 ttatcctaat ctctattgtg tgcaaatctg catatatttt taatcctcta tatttctttt
     1621 attttagcat ggagaacgtg cgtgcttatg gtgccggact agctggcgcc aatttcgaca
     1681 agaacacatt tttcaaaaaa ccaacggttt tgttcaggtg tgctgcattg gtgagttatt
     1741 ttttctgtct ttgtggcaaa taatagacgt aaaaagtatt tctaaaaaac aacgaaaaac
     1801 aaaattgttc tgcctatact ttttgaattt gaaataaata tttttcagct cttcgggctc
     1861 atactttggt actcggtgcc aaaaggtggt tggcacaaac caagcgacgc cattcatccg
     1921 gtatcaaatt acccacccag agacgaattt ggggaagaaa aattattatt tttgacaggt
     1981 gtgcctttat ggaaggagct cgtcaacatg ctcgttcgcc accgccgttg gcttttttgc
     2041 cgtctgtgga gccattgttc taattgttct tgacgccaag atggaccaaa tcagttcggt
     2101 gccaacaaga aggagagctg tcctagcaga tttagttgta tcgggtattt aatttgtgtg
     2161 tggaaaattt gaaagtaaga gaatttcagc aattttcact gccatatttt tgatcggttt
     2221 tttcacattc tggtccaaat tatccgcctt cgaagtcgac gaagacgacg aaaatccaat
     2281 aaagacaaac aacgcgaaat tcggaattct cagtgctctt ctctcctttt tggcttgggt
     2341 gcgtcatgaa aaaagtatag aggaataggg aaaacggaga ggcatctgtt atcttttgac
     2401 agaatcttga gttgaaaagc aacctttttg gccactgtta tcgagtgtta cacatcgtct
     2461 tttaagtgtc aacgtgaaag gttggagaga atttagtgaa atatgatata atttagtcca
     2521 tcagaaagag atttgatact caagcggttt tcagtagagc atcaaataga gaatacttgc
     2581 aaatcaattt tgcacatttt agggaggtgc cgcatttttc gcatggcgtc gttacgaaga
     2641 aggaaaccaa gcaactcatg agcccaatta cgatgaacat tttggacaag tgtcgacaga
     2701 cgtgcaagat ggttatggat acggaggaga ctcaaccggt tagaataatt ttaaacaaat
     2761 agagaaaaaa ctagttgaaa attcttatga aaatcggatc tcaaattcag tttgaaattt
     2821 caaaattctg tgcagaacac aactactcaa ttttttagtt caccactttt tcattgtatt
     2881 cttatcacat tttgttcaac aaatctttag aaaaacgttt tacacgtatt atataatttt
     2941 taagtttatg actcttgaga tattacatgt taaaattgag caaaatagcg tagtactagt
     3001 agaacaaaaa attgatatat caaatatacc ggtgaaacca caaatacttc aaactttgtt
     3061 agaagaatac acaaaaataa tatttacaat aaacttatct gaaacttccc aggaagtttg
     3121 ttcacttttt caaaaaatta tatataagct agttgaaact tgttttacaa tttaaaaaaa
     3181 agcaattttt ttgagttttc tctggaagaa atatttttca taagtgaata tatgatttta
     3241 gtggttttga gattttctgc attgaaaaaa ttcatttttc ttaacatttt ttagcaaaaa
     3301 cttttaaaaa aatcacacgt tgtttttttc aggaatcgga catgttggcg cacctccacc
     3361 tcaatcctca taccaatctg gtgctgcacc acaaaccatg caacaaccac catcaaaccc
     3421 atacactcag tcggaaggat atggttatgg tggcggaggg tctggtggcg gagggtccgg
     3481 tggcggaggg tcaggggtac cgatgaagat ggacaaaaag actatagttt ggtttagaag
     3541 agacctaagg attgaggata atcctgcatt agcagcagct gctcacgaag gatctgtttt
     3601 tcctgtcttc atttggtgtc ctgaagaaga aggacagttt tatcctggaa gagcttcaag
     3661 atggtggatg aaacaatcac ttgctcactt atctcaatcc ttgaaggctc ttggatctga
     3721 cctcacttta atcaaaaccc acaacacgat ttcagcgatc ttggattgta tccgcgttac
     3781 cggtgctaca aaagtcgtct ttaaccacct ctatgatcct gtttcgttag ttcgggacca
     3841 taccgtaaag gagaagctgg tggaacgtgg gatctctgtg caaagctaca atggagatct
     3901 attgtatgaa ccgtgggaga tatactgcga aaagggcaaa ccttttacga gtttcaattc
     3961 ttactggaag aaatgcttag atatgtcgat tgaatccgtt atgcttcctc ctccttggcg
     4021 gttgatgcca ataactgcag cggctgaagc gatttgggcg tgttcgattg aagaactagg
     4081 gctggagaat gaggccgaga aaccgagcaa tgcgttgtta actagagctt ggtctccagg
     4141 atggagcaat gctgataagt tactaaatga gttcatcgag aagcagttga tagattatgc
     4201 aaagaacagc aagaaagttg ttgggaattc tacttcacta ctttctccgt atctccattt
     4261 cggggaaata agcgtcagac acgttttcca gtgtgcccgg atgaaacaaa ttatatgggc
     4321 aagagataag aacagtgaag gagaagaaag tgcagatctt tttcttaggg gaatcggttt
     4381 aagagagtat tctcggtata tatgtttcaa cttcccgttt actcacgagc aatcgttgtt
     4441 gagtcatctt cggtttttcc cttgggatgc tgatgttgat aagttcaagg cctggagaca
     4501 aggcaggacc ggttatccgt tggtggatgc cggaatgaga gagctttggg ctaccggatg
     4561 gatgcataac agaataagag tgattgtttc aagctttgct gtgaagtttc ttctccttcc
     4621 atggaaatgg ggaatgaagt atttctggga tacacttttg gatgctgatt tggaatgtga
     4681 catccttggc tggcagtata tctctgggag tatccccgat ggccacgagc ttgatcgctt
     4741 ggacaatccc gcgttacaag gcgccaaata tgacccagaa ggtgagtaca taaggcaatg
     4801 gcttcccgag cttgcgagat tgccaactga atggatccat catccatggg acgctccttt
     4861 aaccgtactc aaagcttctg gtgtggaact cggaacaaac tatgcgaaac ccattgtaga
     4921 catcgacaca gctcgtgagc tactagctaa agctatttca agaacccgtg gagcacagat
     4981 catgatcgga gcagcacctg atgagattgt agcagatagc ttcgaggcct taggggctaa
     5041 taccattaaa gaacctggtc tttgcccatc tgtgtcttct aatgaccaac aagtaccttc
     5101 ggctgtttaa cctagggcct gcaggcccgg gagcattcgt agaattccaa ctgagcgccg
     5161 gtcgctacca ttaccaactt gtctggtgtc aaaaataata ggggccgctg tcatcagagt
     5221 aagtttaaac tgagttctac taactaacga gtaatattta aattttcagc atctcgcgcc
     5281 cgtgcctctg acttctaagt ccaattactc ttcaacatcc ctacatgctc tttctccctg
     5341 tgctcccacc ccctattttt gttattatca aaaaacttct cttaatttct ttgtttttta
     5401 gcttctttta agtcacctct aacaatgaaa ttgtgtagat tcaaaaatag aattaattcg
     5461 taataaaaag tcgaaaaaaa ttgtgctccc tccccccatt aataataatt ctatcccaaa
     5521 atctacacaa tgttctgtgt acacttctta tgttttttac ttctgataaa tttttttgaa
     5581 acatcataga aaaaaccgca cacaaaatac cttatcatat gttacgtttc agtttatgac
     5641 cgcaattttt atttcttcgc acgtctgggc ctctcatgac gtcaaatcat gctcatcgtg
     5701 aaaaagtttt ggagtatttt tggaattttt caatcaagtg aaagtttatg aaattaattt
     5761 tcctgctttt gctttttggg gtttccccta ttgtttgtca agatttcgag gacggcgttt
     5821 ttcttgctaa aatcacaagt attgatgagc acgatgcaag aaagatcgga agaaggtttg
     5881 ggtttgaggc tcagtggaag gtgagtagaa gttgataatt tgaaagtgga gtagtgtcta
     5941 tggggttttt gccttaaatg acagaataca ttcccaatat accaaacata actgtttcct
     6001 actagtcggc cgtacgggcc ctttcgtctc gcgcgtttcg gtgatgacgg tgaaaacctc
     6061 tgacacatgc agctcccgga gacggtcaca gcttgtctgt aagcggatgc cgggagcaga
     6121 caagcccgtc agggcgcgtc agcgggtgtt ggcgggtgtc ggggctggct taactatgcg
     6181 gcatcagagc agattgtact gagagtgcac catatgcggt gtgaaatacc gcacagatgc
     6241 gtaaggagaa aataccgcat caggcggcct taagggcctc gtgatacgcc tatttttata
     6301 ggttaatgtc atgataataa tggtttctta gacgtcaggt ggcacttttc ggggaaatgt
     6361 gcgcggaacc cctatttgtt tatttttcta aatacattca aatatgtatc cgctcatgag
     6421 acaataaccc tgataaatgc ttcaataata ttgaaaaagg aagagtatga gtattcaaca
     6481 tttccgtgtc gcccttattc ccttttttgc ggcattttgc cttcctgttt ttgctcaccc
     6541 agaaacgctg gtgaaagtaa aagatgctga agatcagttg ggtgcacgag tgggttacat
     6601 cgaactggat ctcaacagcg gtaagatcct tgagagtttt cgccccgaag aacgttttcc
     6661 aatgatgagc acttttaaag ttctgctatg tggcgcggta ttatcccgta ttgacgccgg
     6721 gcaagagcaa ctcggtcgcc gcatacacta ttctcagaat gacttggttg agtactcacc
     6781 agtcacagaa aagcatctta cggatggcat gacagtaaga gaattatgca gtgctgccat
     6841 aaccatgagt gataacactg cggccaactt acttctgaca acgatcggag gaccgaagga
     6901 gctaaccgct tttttgcaca acatggggga tcatgtaact cgccttgatc gttgggaacc
     6961 ggagctgaat gaagccatac caaacgacga gcgtgacacc acgatgcctg tagcaatggc
     7021 aacaacgttg cgcaaactat taactggcga actacttact ctagcttccc ggcaacaatt
     7081 aatagactgg atggaggcgg ataaagttgc aggaccactt ctgcgctcgg cccttccggc
     7141 tggctggttt attgctgata aatctggagc cggtgagcgt gggtctcgcg gtatcattgc
     7201 agcactgggg ccagatggta agccctcccg tatcgtagtt atctacacga cggggagtca
     7261 ggcaactatg gatgaacgaa atagacagat cgctgagata ggtgcctcac tgattaagca
     7321 ttggtaactg tcagaccaag tttactcata tatactttag attgatttaa aacttcattt
     7381 ttaatttaaa aggatctagg tgaagatcct ttttgataat ctcatgacca aaatccctta
     7441 acgtgagttt tcgttccact gagcgtcaga ccccgtagaa aagatcaaag gatcttcttg
     7501 agatcctttt tttctgcgcg taatctgctg cttgcaaaca aaaaaaccac cgctaccagc
     7561 ggtggtttgt ttgccggatc aagagctacc aactcttttt ccgaaggtaa ctggcttcag
     7621 cagagcgcag ataccaaata ctgttcttct agtgtagccg tagttaggcc accacttcaa
     7681 gaactctgta gcaccgccta catacctcgc tctgctaatc ctgttaccag tggctgctgc
     7741 cagtggcgat aagtcgtgtc ttaccgggtt ggactcaaga cgatagttac cggataaggc
     7801 gcagcggtcg ggctgaacgg ggggttcgtg cacacagccc agcttggagc gaacgaccta
     7861 caccgaactg agatacctac agcgtgagct atgagaaagc gccacgcttc ccgaagggag
     7921 aaaggcggac aggtatccgg taagcggcag ggtcggaaca ggagagcgca cgagggagct
     7981 tccaggggga aacgcctggt atctttatag tcctgtcggg tttcgccacc tctgacttga
     8041 gcgtcgattt ttgtgatgct cgtcaggggg gcggagccta tggaaaaacg ccagcaacgc
     8101 ggccttttta cggttcctgg ccttttgctg gccttttgct cacatgttct ttcctgcgtt
     8161 atcccctgat tctgtggata accgtattac cgcctttgag tgagctgata ccgctcgccg
     8221 cagccgaacg accgagcgca gcgagtcagt gagcgaggaa gcggaagagc gcccaatacg
     8281 caaaccgcct ctccccgcgc gttggccgat tcattaatgc agctggcacg acaggtttcc
     8341 cgactggaaa gcgggcagtg agcgcaacgc aattaatgtg agttagctca ctcattaggc
     8401 accccaggct ttacacttta tgcttccggc tcgtatgttg tgtggaattg tgagcggata
     8461 acaatttcac acaggaaaca gctatgacca tgattacgcc aagctgtaag tttaaacatg
     8521 atcttactaa ctaactattc tcatttaaat tttcagagct taaaaatggc tgaaatcact
     8581 cacaacgatg gatacgctaa caacttggaa atgaaataag cttgcatgc
//
