LOCUS       sequence-410709-       11804 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Gateway destination clone of CDK11B (human) tagged with C-terminal 
            V5-miniTurbo for generating protein-proximity networks using 
            proximity-dependent biotinylation proteomics.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11804)
  TITLE     Major lab plasmids for proteomics-based protein-interaction and 
            protein-proximity network generation
REFERENCE   2  (bases 1 to 11804)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: p667-UBC-CDK11B-V5-miniTurbo_IDG-K
FEATURES             Location/Qualifiers
     source          1..11804
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        26..130
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(131..199,200..991)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     rep_origin      1162..1750
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     1651..1670
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     1904..1921
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    2038..2059
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(2074..2079,2080..2097,2098..2104)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    2112..2128
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     2117..2139
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     2136..2152
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     2136..2152
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     2171..2191
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        2173..2191
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        2219..2445
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             2446..2626
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    2673..2798
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    3291..3524
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             3709..3753
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    4020..4136
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     protein_bind    4168..4188
                     /gene="mutant version of attB"
                     /label=attB4
                     /bound_moiety="BP Clonase(TM)"
                     /note="core recombination site for the Gateway(R) BP 
                     reaction"
     promoter        4188..5399
                     /label=UbC promoter
                     /note="human ubiquitin C promoter"
     primer_bind     5236..5255
                     /label=hUBCpro-F
                     /note="Human Ubiquitin C (UbC) promoter, forward primer"
     protein_bind    5400..5424
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     regulatory      5427..5436
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     protein_bind    complement(7783..7807)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             7809..7850
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     CDS             7857..8627
                     /codon_start=1
                     /product="N-terminally truncated mutant of the E. coli 
                     biotin ligase BioID engineered for improved catalytic 
                     efficiency (Branon et al., 2018)"
                     /label=miniTurbo
                     /translation="IPLLNAKQILGQLDGGSVAVLPVVDSTNQYLLDRIGELKSGDACI
                     AEYQQAGRGSRGRKWFSPFGANLYLSMFWRLKRGPAAIGLGPVIGIVMAEALRKLGADK
                     VRVKWPNDLYLQDRKLAGILVELAGITGDAAQIVIGAGINVAMRRVEESVVNQGWITLQ
                     EAGINLDRNTLAAMLIRELRAALELFEQEGLAPYLSRWEKLDNFINRPVKLIIGDKEIF
                     GISRGIDKQGALLLEQDGVIKPWMGGEISLRSAEK"
     protein_bind    complement(8633..8653)
                     /gene="mutant version of attB"
                     /label=attB3
                     /bound_moiety="BP Clonase(TM)"
                     /note="core recombination site for the Gateway(R) BP 
                     reaction"
     misc_feature    8682..9270
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(8735..8755)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(9153..9164)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     promoter        9284..9783
                     /label=PGK promoter
                     /note="mouse phosphoglycerate kinase 1 promoter"
     primer_bind     complement(9319..9342)
                     /label=MSCV-rev
                     /note="Murine stem cell virus, reverse primer"
     primer_bind     9716..9735
                     /label=mPGK-F
                     /note="Mouse PGK promoter, forward primer"
     CDS             9804..10403
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(9804..9823)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     10300..10320
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     LTR             10541..10774
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    10846..10967
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(10883..10902)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     10937..10956
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      11007..11142
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(11162..11181)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(11163..11181)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(11191..11208)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(11191..11207)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(11200..11222)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      11349..11777
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(11436..11455)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     11646..11667
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
ORIGIN
        1 aggtggcact tttcggggaa atgtgcgcgg aacccctatt tgtttatttt tctaaataca
       61 ttcaaatatg tatccgctca tgagacaata accctgataa atgcttcaat aatattgaaa
      121 aaggaagagt atgagtattc aacatttccg tgtcgccctt attccctttt ttgcggcatt
      181 ttgccttcct gtttttgctc acccagaaac gctggtgaaa gtaaaagatg ctgaagatca
      241 gttgggtgca cgagtgggtt acatcgaact ggatctcaac agcggtaaga tccttgagag
      301 ttttcgcccc gaagaacgtt ttccaatgat gagcactttt aaagttctgc tatgtggcgc
      361 ggtattatcc cgtattgacg ccgggcaaga gcaactcggt cgccgcatac actattctca
      421 gaatgacttg gttgagtact caccagtcac agaaaagcat cttacggatg gcatgacagt
      481 aagagaatta tgcagtgctg ccataaccat gagtgataac actgcggcca acttacttct
      541 gacaacgatc ggaggaccga aggagctaac cgcttttttg cacaacatgg gggatcatgt
      601 aactcgcctt gatcgttggg aaccggagct gaatgaagcc ataccaaacg acgagcgtga
      661 caccacgatg cctgtagcaa tggcaacaac gttgcgcaaa ctattaactg gcgaactact
      721 tactctagct tcccggcaac aattaataga ctggatggag gcggataaag ttgcaggacc
      781 acttctgcgc tcggcccttc cggctggctg gtttattgct gataaatctg gagccggtga
      841 gcgtgggtct cgcggtatca ttgcagcact ggggccagat ggtaagccct cccgtatcgt
      901 agttatctac acgacgggga gtcaggcaac tatggatgaa cgaaatagac agatcgctga
      961 gataggtgcc tcactgatta agcattggta actgtcagac caagtttact catatatact
     1021 ttagattgat ttaaaacttc atttttaatt taaaaggatc taggtgaaga tcctttttga
     1081 taatctcatg accaaaatcc cttaacgtga gttttcgttc cactgagcgt cagaccccgt
     1141 agaaaagatc aaaggatctt cttgagatcc tttttttctg cgcgtaatct gctgcttgca
     1201 aacaaaaaaa ccaccgctac cagcggtggt ttgtttgccg gatcaagagc taccaactct
     1261 ttttccgaag gtaactggct tcagcagagc gcagatacca aatactgttc ttctagtgta
     1321 gccgtagtta ggccaccact tcaagaactc tgtagcaccg cctacatacc tcgctctgct
     1381 aatcctgtta ccagtggctg ctgccagtgg cgataagtcg tgtcttaccg ggttggactc
     1441 aagacgatag ttaccggata aggcgcagcg gtcgggctga acggggggtt cgtgcacaca
     1501 gcccagcttg gagcgaacga cctacaccga actgagatac ctacagcgtg agctatgaga
     1561 aagcgccacg cttcccgaag ggagaaaggt ggacaggtat ccggtaagcg gcagggtcgg
     1621 aacaggagag cgcacgaggg agcttccagg gggaaacgcc tggtatcttt atagtcctgt
     1681 cgggtttcgc cacctctgac ttgagcgtcg atttttgtga tgctcgtcag gggggcggag
     1741 cctatggaaa aacgccagca acgcggcctt tttacggttc ctggcctttt gctggccttt
     1801 tgctcacatg ttctttcctg cgttatcccc tgattctgtg gataaccgta ttaccgcctt
     1861 tgagtgagct gataccgctc gccgcagccg aacgaccgag cgcagcgagt cagtgagcga
     1921 ggaagcggaa gagcgcccaa tacgcaaacc gcctctcccc gcgcgttggc cgattcatta
     1981 atgcagctgg cacgacaggt ttcccgactg gaaagcgggc agtgagcgca acgcaattaa
     2041 tgtgagttag ctcactcatt aggcacccca ggctttacac tttatgcttc cggctcgtat
     2101 gttgtgtgga attgtgagcg gataacaatt tcacacagga aacagctatg accatgatta
     2161 cgccaagcgc gcaattaacc ctcactaaag ggaacaaaag ctggagctgc aagcttaatg
     2221 tagtcttatg caatactctt gtagtcttgc aacatggtaa cgatgagtta gcaacatgcc
     2281 ttacaaggag agaaaaagca ccgtgcatgc cgattggtgg aagtaaggtg gtacgatcgt
     2341 gccttattag gaaggcaaca gacgggtctg acatggattg gacgaaccac tgaattgccg
     2401 cattgcagag atattgtatt taagtgccta gctcgataca taaacgggtc tctctggtta
     2461 gaccagatct gagcctggga gctctctggc taactaggga acccactgct taagcctcaa
     2521 taaagcttgc cttgagtgct tcaagtagtg tgtgcccgtc tgttgtgtga ctctggtaac
     2581 tagagatccc tcagaccctt ttagtcagtg tggaaaatct ctagcagtgg cgcccgaaca
     2641 gggacttgaa agcgaaaggg aaaccagagg agctctctcg acgcaggact cggcttgctg
     2701 aagcgcgcac ggcaagaggc gaggggcggc gactggtgag tacgccaaaa attttgacta
     2761 gcggaggcta gaaggagaga gatgggtgcg agagcgtcag tattaagcgg gggagaatta
     2821 gatcgcgatg ggaaaaaatt cggttaaggc cagggggaaa gaaaaaatat aaattaaaac
     2881 atatagtatg ggcaagcagg gagctagaac gattcgcagt taatcctggc ctgttagaaa
     2941 catcagaagg ctgtagacaa atactgggac agctacaacc atcccttcag acaggatcag
     3001 aagaacttag atcattatat aatacagtag caaccctcta ttgtgtgcat caaaggatag
     3061 agataaaaga caccaaggaa gctttagaca agatagagga agagcaaaac aaaagtaaga
     3121 ccaccgcaca gcaagcggcc gctgatcttc agacctggag gaggagatat gagggacaat
     3181 tggagaagtg aattatataa atataaagta gtaaaaattg aaccattagg agtagcaccc
     3241 accaaggcaa agagaagagt ggtgcagaga gaaaaaagag cagtgggaat aggagctttg
     3301 ttccttgggt tcttgggagc agcaggaagc actatgggcg cagcgtcaat gacgctgacg
     3361 gtacaggcca gacaattatt gtctggtata gtgcagcagc agaacaattt gctgagggct
     3421 attgaggcgc aacagcatct gttgcaactc acagtctggg gcatcaagca gctccaggca
     3481 agaatcctgg ctgtggaaag atacctaaag gatcaacagc tcctggggat ttggggttgc
     3541 tctggaaaac tcatttgcac cactgctgtg ccttggaatg ctagttggag taataaatct
     3601 ctggaacaga tttggaatca cacgacctgg atggagtggg acagagaaat taacaattac
     3661 acaagcttaa tacactcctt aattgaagaa tcgcaaaacc agcaagaaaa gaatgaacaa
     3721 gaattattgg aattagataa atgggcaagt ttgtggaatt ggtttaacat aacaaattgg
     3781 ctgtggtata taaaattatt cataatgata gtaggaggct tggtaggttt aagaatagtt
     3841 tttgctgtac tttctatagt gaatagagtt aggcagggat attcaccatt atcgtttcag
     3901 acccacctcc caaccccgag gggacccgac aggcccgaag gaatagaaga agaaggtgga
     3961 gagagagaca gagacagatc cattcgatta gtgaacggat ctcgacggta tcggttaact
     4021 tttaaaagaa aaggggggat tggggggtac agtgcagggg aaagaatagt agacataata
     4081 gcaacagaca tacaaactaa agaattacaa aaacaaatta caaaattcaa aattttatcg
     4141 tccagtgtgg tggaattctg cagatatcaa ctttgtatag aaaagttggc ctccgcgccg
     4201 ggttttggcg cctcccgcgg gcgcccccct cctcacggcg agcgctgcca cgtcagacga
     4261 agggcgcagc gagcgtcctg atccttccgc ccggacgctc aggacagcgg cccgctgctc
     4321 ataagactcg gccttagaac cccagtatca gcagaaggac attttaggac gggacttggg
     4381 tgactctagg gcactggttt tctttccaga gagcggaaca ggcgaggaaa agtagtccct
     4441 tctcggcgat tctgcggagg gatctccgtg gggcggtgaa cgccgatgat tatataagga
     4501 cgcgccgggt gtggcacagc tagttccgtc gcagccggga tttgggtcgc ggttcttgtt
     4561 tgtggatcgc tgtgatcgtc acttggtgag tagcgggctg ctgggctggc cggggctttc
     4621 gtggccgccg ggccgctcgg tgggacggaa gcgtgtggag agaccgccaa gggctgtagt
     4681 ctgggtccgc gagcaaggtt gccctgaact gggggttggg gggagcgcag caaaatggcg
     4741 gctgttcccg agtcttgaat ggaagacgct tgtgaggcgg gctgtgaggt cgttgaaaca
     4801 aggtgggggg catggtgggc ggcaagaacc caaggtcttg aggccttcgc taatgcggga
     4861 aagctcttat tcgggtgaga tgggctgggg caccatctgg ggaccctgac gtgaagtttg
     4921 tcactgactg gagaactcgg tttgtcgtct gttgcggggg cggcagttat ggcggtgccg
     4981 ttgggcagtg cacccgtacc tttgggagcg cgcgccctcg tcgtgtcgtg acgtcacccg
     5041 ttctgttggc ttataatgca gggtggggcc acctgccggt aggtgtgcgg taggcttttc
     5101 tccgtcgcag gacgcagggt tcgggcctag ggtaggctct cctgaatcga caggcgccgg
     5161 acctctggtg aggggaggga taagtgaggc gtcagtttct ttggtcggtt ttatgtacct
     5221 atcttcttaa gtagctgaag ctccggtttt gaactatgcg ctcggggttg gcgagtgtgt
     5281 tttgtgaagt tttttaggca ccttttgaaa tgtaatcatt tgggtcaata tgtaattttc
     5341 agtgttagac tagtaaattg tccgctaaat tctggccgtt tttggctttt ttgttagaca
     5401 caagtttgta caaaaaagca ggcttagcca ccatgggtga tgaaaaggac tcttggaaag
     5461 tgaaaacttt agatgaaatt cttcaggaaa agaaacgaag gaaggaacaa gaggagaaag
     5521 cagagataaa acgcttaaaa aattctgatg accgggattc caagcgggat tcccttgagg
     5581 agggggagct gagagatcac cgcatggaga tcacaataag gaactccccg tatagaagag
     5641 aagactctat ggaagacaga ggagaagaag atgattcttt ggccatcaaa ccaccccagc
     5701 aaatgtctcg gaaagaaaaa gctcatcaca gaaaagatga aaagagaaaa gagaaacgta
     5761 ggcatcgtag ccattcagca gaagggggga agcatgctag agtgaaagaa aaagaaagag
     5821 agcacgaacg tcggaaacgg catcgagaag aacaggataa agctcgccgg gaatgggaaa
     5881 gacagaagag aagggagatg gcaagggagc attccaggag agaaagggac cgcttggagc
     5941 agttagaaag gaagcgggag cgggagcgca agatgcggga gcagcagaag gagcagcggg
     6001 agcagaagga gcgcgagcgg cgggcggagg agcggcgcaa ggagcgggag gcccgcaggg
     6061 aagtgtctgc acatcaccga acgatgagag aggactacag cgacaaagtg aaagccagcc
     6121 actggagtcg cagcccgcct cggccgccgc gggagcggtt cgagttggga gacggccgga
     6181 agccagtaaa agaagagaaa atggaagaaa gggacctgct gtccgactta caggacatca
     6241 gcgacagcga gaggaagacc agctcggccg agtcctcgtc agcggaatca ggctcaggtt
     6301 ctgaggaaga agaggaggag gaggaagagg aggaggagga agggagcacc agtgaagaat
     6361 cagaggagga ggaggaggaa gaggaagagg aggaggagga gaccggcagc aactctgagg
     6421 aggcatcaga gcagtctgcc gaagaagtaa gtgaggaaga aatgagtgaa gatgaagaac
     6481 gagaaaatga aaaccacctc ttggttgttc cagagtcacg gttcgaccga gattccgggg
     6541 agagtgaaga agcagaggaa gaagtgggtg agggaacgcc gcagagcagc gccctgacag
     6601 agggcgacta tgtgcccgac tcccctgccc tgtcgcccat cgagctcaag caggagctgc
     6661 ccaagtacct gccggccctg cagggctgcc ggagcgtcga ggagttccag tgcctgaaca
     6721 ggatcgagga gggcacctat ggagtggtct acagagcaaa agacaagaaa acagatgaaa
     6781 ttgtggctct aaagcggctg aagatggaga aggagaagga gggcttcccg atcacgtcgc
     6841 tgagggagat caacaccatc ctcaaggccc agcatcccaa catcgtcacc gttagagaga
     6901 ttgtggtggg cagcaacatg gacaagatct acatcgtgat gaactatgtg gagcacgacc
     6961 tcaagagcct gatggagacc atgaaacagc ccttcctgcc aggggaggtg aagaccctga
     7021 tgatccagct gctgcgtggg gtgaaacacc tgcacgacaa ctggatcctg caccgtgacc
     7081 tcaagacgtc caacctgctg ctgagccacg ccggcatcct caaggtgggt gacttcgggc
     7141 tggcgcggga gtacggatcc cctctgaagg cctacacccc ggtcgtggtg accctgtggt
     7201 accgcgcccc agagctgctg cttggtgcca aggaatactc cacggccgtg gacatgtggt
     7261 cagtgggttg catcttcggg gagctgctga ctcagaagcc tctgttcccc gggaagtcag
     7321 aaatcgatca gatcaacaag gtgttcaagg atctggggac ccctagtgag aaaatctggc
     7381 ccggctacag cgagctccca gcagtcaaga agatgacctt cagcgagcac ccctacaaca
     7441 acctccgcaa gcgcttcggg gctctgctct cagaccaggg cttcgacctc atgaacaagt
     7501 tcctgaccta cttccccggg aggaggatca gcgctgagga cggcctcaag catgagtatt
     7561 tccgcgagac ccccctcccc atcgacccct ccatgttccc cacgtggccc gccaagagcg
     7621 agcagcagcg tgtgaagcgg ggcaccagcc cgaggccccc tgagggaggc ctgggctaca
     7681 gccagctggg tgacgacgac ctgaaggaga cgggcttcca ccttaccacc acgaaccagg
     7741 gggcctctgc cgcgggcccc ggcttcagcc tcaagttctc aaacccagct ttcttgtaca
     7801 aagtgggcgg caagcccatc cccaaccccc tgctgggcct ggacagcacc gctagcatcc
     7861 cgctgctgaa cgctaaacag attctgggac agctggacgg cgggagcgtg gcagtcctgc
     7921 ctgtggtcga ctccaccaat cagtacctgc tggatcgaat cggcgagctg aagagtgggg
     7981 atgcttgcat tgcagaatat cagcaggcag ggagaggaag cagagggagg aaatggttct
     8041 ctccttttgg agctaacctg tacctgagta tgttttggcg cctgaagcgg ggaccagcag
     8101 caatcggcct gggcccggtc atcggaattg tcatggcaga agcgctgcga aagctgggag
     8161 cagacaaggt gcgagtcaaa tggcccaatg acctgtatct gcaggataga aagctggcag
     8221 gcatcctggt ggagctggcc ggaataacag gcgatgctgc acagatcgtc attggcgccg
     8281 ggattaacgt ggctatgagg cgcgtggagg aaagcgtggt caatcagggc tggatcacac
     8341 tgcaggaagc agggattaac ctggacagga atactctggc cgctatgctg atccgagagc
     8401 tgcgggcagc cctggaactg ttcgagcagg aaggcctggc tccatatctg tcacggtggg
     8461 agaagctgga taacttcatc aatagacccg tgaagctgat cattggggac aaagagattt
     8521 tcgggattag ccgggggatt gataaacagg gagccctgct gctggaacag gacggagtta
     8581 tcaaaccctg gatgggcgga gaaatcagtc tgcggtctgc cgaaaagtaa gccaacttta
     8641 ttatacatag ttgatatcca gcacagtggc ggccgctcga caatcaacct ctggattaca
     8701 aaatttgtga aagattgact ggtattctta actatgttgc tccttttacg ctatgtggat
     8761 acgctgcttt aatgcctttg tatcatgcta ttgcttcccg tatggctttc attttctcct
     8821 ccttgtataa atcctggttg ctgtctcttt atgaggagtt gtggcccgtt gtcaggcaac
     8881 gtggcgtggt gtgcactgtg tttgctgacg caacccccac tggttggggc attgccacca
     8941 cctgtcagct cctttccggg actttcgctt tccccctccc tattgccacg gcggaactca
     9001 tcgccgcctg ccttgcccgc tgctggacag gggctcggct gttgggcact gacaattccg
     9061 tggtgttgtc ggggaagctg acgtcctttc catggctgct cgcctgtgtt gccacctgga
     9121 ttctgcgcgg gacgtccttc tgctacgtcc cttcggccct caatccagcg gaccttcctt
     9181 cccgcggcct gctgccggct ctgcggcctc ttccgcgtct tcgccttcgc cctcagacga
     9241 gtcggatctc cctttgggcc gcctccccgc ctggaattct accgggtagg ggaggcgctt
     9301 ttcccaaggc agtctggagc atgcgcttta gcagccccgc tgggcacttg gcgctacaca
     9361 agtggcctct ggcctcgcac acattccaca tccaccggta ggcgccaacc ggctccgttc
     9421 tttggtggcc ccttcgcgcc accttctact cctcccctag tcaggaagtt cccccccgcc
     9481 ccgcagctcg cgtcgtgcag gacgtgacaa atggaagtag cacgtctcac tagtctcgtg
     9541 cagatggaca gcaccgctga gcaatggaag cgggtaggcc tttggggcag cggccaatag
     9601 cagctttgct ccttcgcttt ctgggctcag aggctgggaa ggggtgggtc cgggggcggg
     9661 ctcaggggcg ggctcagggg cggggcgggc gcccgaaggt cctccggagg cccggcattc
     9721 tgcacgcttc aaaagcgcac gtctgccgcg ctgttctcct cttcctcatc tccgggcctt
     9781 tcgacctgca gcccaagctt accatgaccg agtacaagcc cacggtgcgc ctcgccaccc
     9841 gcgacgacgt ccccagggcc gtacgcaccc tcgccgccgc gttcgccgac taccccgcca
     9901 cgcgccacac cgtcgatccg gaccgccaca tcgagcgggt caccgagctg caagaactct
     9961 tcctcacgcg cgtcgggctc gacatcggca aggtgtgggt cgcggacgac ggcgccgcgg
    10021 tggcggtctg gaccacgccg gagagcgtcg aagcgggggc ggtgttcgcc gagatcggcc
    10081 cgcgcatggc cgagttgagc ggttcccggc tggccgcgca gcaacagatg gaaggcctcc
    10141 tggcgccgca ccggcccaag gagcccgcgt ggttcctggc caccgtcggc gtctcgcccg
    10201 accaccaggg caagggtctg ggcagcgccg tcgtgctccc cggagtggag gcggccgagc
    10261 gcgccggggt gcccgccttc ctggagacct ccgcgccccg caacctcccc ttctacgagc
    10321 ggctcggctt caccgtcacc gccgacgtcg aggtgcccga aggaccgcgc acctggtgca
    10381 tgacccgcaa gcccggtgcc tgacgcccgc cccacgaccc gcagcgcccg accgaaagga
    10441 gcgcacgacc ccatgcatct cgagggcccg gtacctttaa gaccaatgac ttacaaggca
    10501 gctgtagatc ttagccactt tttaaaagaa aaggggggac tggaagggct agctcactcc
    10561 caacgaagac aagatctgct ttttgcttgt actgggtctc tctggttaga ccagatctga
    10621 gcctgggagc tctctggcta actagggaac ccactgctta agcctcaata aagcttgcct
    10681 tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta gagatccctc
    10741 agaccctttt agtcagtgtg gaaaatctct agcagtagta gttcatgtca tcttattatt
    10801 cagtatttat aacttgcaaa gaaatgaata tcagagagtg agaggaactt gtttattgca
    10861 gcttataatg gttacaaata aagcaatagc atcacaaatt tcacaaataa agcatttttt
    10921 tcactgcatt ctagttgtgg tttgtccaaa ctcatcaatg tatcttatca tgtctggctc
    10981 tagctatccc gcccctaact ccgcccatcc cgcccctaac tccgcccagt tccgcccatt
    11041 ctccgcccca tggctgacta atttttttta tttatgcaga ggccgaggcc gcctcggcct
    11101 ctgagctatt ccagaagtag tgaggaggct tttttggagg cctagggacg tacccaattc
    11161 gccctatagt gagtcgtatt acgcgcgctc actggccgtc gttttacaac gtcgtgactg
    11221 ggaaaaccct ggcgttaccc aacttaatcg ccttgcagca catccccctt tcgccagctg
    11281 gcgtaatagc gaagaggccc gcaccgatcg cccttcccaa cagttgcgca gcctgaatgg
    11341 cgaatgggac gcgccctgta gcggcgcatt aagcgcggcg ggtgtggtgg ttacgcgcag
    11401 cgtgaccgct acacttgcca gcgccctagc gcccgctcct ttcgctttct tcccttcctt
    11461 tctcgccacg ttcgccggct ttccccgtca agctctaaat cgggggctcc ctttagggtt
    11521 ccgatttagt gctttacggc acctcgaccc caaaaaactt gattagggtg atggttcacg
    11581 tagtgggcca tcgccctgat agacggtttt tcgccctttg acgttggagt ccacgttctt
    11641 taatagtgga ctcttgttcc aaactggaac aacactcaac cctatctcgg tctattcttt
    11701 tgatttataa gggattttgc cgatttcggc ctattggtta aaaaatgagc tgatttaaca
    11761 aaaatttaac gcgaatttta acaaaatatt aacgcttaca attt
//
