LOCUS       sequence-410731-        8743 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  express beta-ACTIN-3′UTR-24xMS2 .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8743)
  AUTHORS   Hu Y, Xu J, Gao E, Fan X, Wei J, Ye B, Xu S, Ma W
  TITLE     Enhanced single RNA imaging reveals dynamic gene expression in live 
            animals.
  JOURNAL   Elife. 2023 Mar 3;12:e82178. doi: 10.7554/eLife.82178.
  PUBMED    36867026
REFERENCE   2  (bases 1 to 8743)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Elife."; 
            date: "2023-03-3"; volume: "12:e82178. doi"; pages: " 
            10.7554/eLife.82178"
COMMENT     Sequence Label: pcDNA-puro-TagBFP-beta-ACTIN-3′UTR-24xMS2
FEATURES             Location/Qualifiers
     source          1..8743
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(44..63)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        235..614
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        615..818
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     769..789
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     863..882
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        863..881
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             905..1603
                     /codon_start=1
                     /product="monomeric blue fluorescent protein"
                     /label=TagBFP
                     /note="mammalian codon-optimized"
                     /translation="MSELIKENMHMKLYMEGTVDNHHFKCTSEGEGKPYEGTQTMRIKV
                     VEGGPLPFAFDILATSFLYGSKTFINHTQGIPDFFKQSFPEGFTWERVTTYEDGGVLTA
                     TQDTSLQDGCLIYNVKIRGVNFTSNGPVMQKKTLGWEAFTETLYPADGGLEGRNDMALK
                     LVGGSHLIANIKTTYRSKKPAKNLKMPGVYYVDYRLERIKEANNETYVEQHEVAVARYC
                     DLPSKLGHKLN"
     misc_RNA        3192..3210
                     /label=MS2 stem loop
                     /note="stem loop that binds the bacteriophage MS2 coat 
                     protein"
     misc_RNA        3231..3249
                     /label=MS2 stem loop
                     /note="stem loop that binds the bacteriophage MS2 coat 
                     protein"
     misc_RNA        3301..3319
                     /label=MS2 stem loop
                     /note="stem loop that binds the bacteriophage MS2 coat 
                     protein"
     misc_RNA        3340..3358
                     /label=MS2 stem loop
                     /note="stem loop that binds the bacteriophage MS2 coat 
                     protein"
     misc_RNA        3410..3428
                     /label=MS2 stem loop
                     /note="stem loop that binds the bacteriophage MS2 coat 
                     protein"
     misc_RNA        3449..3467
                     /label=MS2 stem loop
                     /note="stem loop that binds the bacteriophage MS2 coat 
                     protein"
     misc_RNA        3519..3537
                     /label=MS2 stem loop
                     /note="stem loop that binds the bacteriophage MS2 coat 
                     protein"
     misc_RNA        3558..3576
                     /label=MS2 stem loop
                     /note="stem loop that binds the bacteriophage MS2 coat 
                     protein"
     misc_RNA        3628..3646
                     /label=MS2 stem loop
                     /note="stem loop that binds the bacteriophage MS2 coat 
                     protein"
     misc_RNA        3667..3685
                     /label=MS2 stem loop
                     /note="stem loop that binds the bacteriophage MS2 coat 
                     protein"
     misc_RNA        3737..3755
                     /label=MS2 stem loop
                     /note="stem loop that binds the bacteriophage MS2 coat 
                     protein"
     misc_RNA        3776..3794
                     /label=MS2 stem loop
                     /note="stem loop that binds the bacteriophage MS2 coat 
                     protein"
     misc_RNA        3881..3899
                     /label=MS2 stem loop
                     /note="stem loop that binds the bacteriophage MS2 coat 
                     protein"
     misc_RNA        3920..3938
                     /label=MS2 stem loop
                     /note="stem loop that binds the bacteriophage MS2 coat 
                     protein"
     misc_RNA        3990..4008
                     /label=MS2 stem loop
                     /note="stem loop that binds the bacteriophage MS2 coat 
                     protein"
     misc_RNA        4029..4047
                     /label=MS2 stem loop
                     /note="stem loop that binds the bacteriophage MS2 coat 
                     protein"
     misc_RNA        4099..4117
                     /label=MS2 stem loop
                     /note="stem loop that binds the bacteriophage MS2 coat 
                     protein"
     misc_RNA        4138..4156
                     /label=MS2 stem loop
                     /note="stem loop that binds the bacteriophage MS2 coat 
                     protein"
     misc_RNA        4208..4226
                     /label=MS2 stem loop
                     /note="stem loop that binds the bacteriophage MS2 coat 
                     protein"
     misc_RNA        4247..4265
                     /label=MS2 stem loop
                     /note="stem loop that binds the bacteriophage MS2 coat 
                     protein"
     misc_RNA        4317..4335
                     /label=MS2 stem loop
                     /note="stem loop that binds the bacteriophage MS2 coat 
                     protein"
     misc_RNA        4356..4374
                     /label=MS2 stem loop
                     /note="stem loop that binds the bacteriophage MS2 coat 
                     protein"
     misc_RNA        4426..4444
                     /label=MS2 stem loop
                     /note="stem loop that binds the bacteriophage MS2 coat 
                     protein"
     misc_RNA        4465..4483
                     /label=MS2 stem loop
                     /note="stem loop that binds the bacteriophage MS2 coat 
                     protein"
     primer_bind     complement(4583..4600)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    4589..4813
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      4859..5287
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(4946..4965)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     5156..5177
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(5296..5316)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        5301..5630
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      5481..5616
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     5543..5562
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     CDS             5643..6242
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(5643..5662)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     6139..6159
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     polyA_signal    6416..6537
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(6453..6472)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     6507..6526
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(6586..6602)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(6586..6602)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(6599..6621)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    6610..6626
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(6634..6640,6641..6658,6659..6664))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    6679..6700
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(6817..6834)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(6988..7576)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(7068..7087)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(7747..8538,8539..8607))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     8370..8389
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(8608..8712)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 gacggatcgg gagatctccc gatcccctat ggtgcactct cagtacaatc tgctctgatg
       61 ccgcatagtt aagccagtat ctgctccctg cttgtgtgtt ggaggtcgct gagtagtgcg
      121 cgagcaaaat ttaagctaca acaaggcaag gcttgaccga caattgcatg aagaatctgc
      181 ttagggttag gcgttttgcg ctgcttcgcg atgtacgggc cagatatacg cgttgacatt
      241 gattattgac tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata
      301 tggagttccg cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc
      361 cccgcccatt gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc
      421 attgacgtca atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt
      481 atcatatgcc aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt
      541 atgcccagta catgacctta tgggactttc ctacttggca gtacatctac gtattagtca
      601 tcgctattac catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg
      661 actcacgggg atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc
      721 aaaatcaacg ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg
      781 gtaggcgtgt acggtgggag gtctatataa gcagagctct ctggctaact agagaaccca
      841 ctgcttactg gcttatcgaa attaatacga ctcactatag ggagacccaa gctggctagc
      901 aaccatgagc gagctgatta aggagaacat gcacatgaag ctctacatgg agggcaccgt
      961 ggacaaccat cacttcaagt gcacatccga gggcgaaggc aagccctacg agggcaccca
     1021 gaccatgaga atcaaggtgg tcgagggcgg ccctctcccc ttcgccttcg acatcctggc
     1081 tactagcttc ctctacggca gcaagacctt catcaaccac acccagggca tccccgactt
     1141 cttcaagcag tccttccctg agggcttcac atgggagaga gtcaccacat acgaagacgg
     1201 gggcgtgctg accgctaccc aggacaccag cctccaggac ggctgcctca tctacaacgt
     1261 caagatcaga ggggtgaact tcacatccaa cggccctgtg atgcagaaga aaacactcgg
     1321 ctgggaggcc ttcaccgaga cgctgtaccc cgctgacggc ggcctggaag gcagaaacga
     1381 catggccctg aagctcgtgg gcgggagcca tctgatcgca aacatcaaga ccacatatag
     1441 atccaagaaa cccgctaaga acctcaagat gcctggcgtc tactatgtgg actacagact
     1501 ggaaagaatc aaggaggcca acaacgagac ctacgtcgag cagcacgagg tggcagtggc
     1561 cagatactgc gacctcccta gcaaactggg gcacaagctg aatctgtaca agaagcttgg
     1621 taccgatgat gatatcgccg cgctcgtcgt cgacaacggc tccggcatgt gcaaggccgg
     1681 cttcgcgggc gacgatgccc cccgggccgt cttcccctcc atcgtggggc gccccaggca
     1741 ccagggcgtg atggtgggca tgggtcagaa ggattcctat gtgggcgacg aggcccagag
     1801 caagagaggc atcctcaccc tgaagtaccc catcgagcac ggcatcgtca ccaactggga
     1861 cgacatggag aaaatctggc accacacctt ctacaatgag ctgcgtgtgg ctcccgagga
     1921 gcaccccgtg ctgctgaccg aggcccccct gaaccccaag gccaaccgcg agaagatgac
     1981 ccagatcatg tttgagacct tcaacacccc agccatgtac gttgctatcc aggctgtgct
     2041 atccctgtac gcctctggcc gtaccactgg catcgtgatg gactccggtg acggggtcac
     2101 ccacactgtg cccatctacg aggggtatgc cctcccccat gccatcctgc gtctggacct
     2161 ggctggccgg gacctgactg actacctcat gaagatcctc accgagcgcg gctacagctt
     2221 caccaccacg gccgagcggg aaatcgtgcg tgacattaag gagaagctgt gctacgtcgc
     2281 cctggacttc gagcaagaga tggccacggc tgcttccagc tcctccctgg agaagagcta
     2341 cgagctgcct gacggccagg tcatcaccat tggcaatgag cggttccgct gccctgaggc
     2401 actcttccag ccttccttcc tgggcatgga gtcctgtggc atccacgaaa ctaccttcaa
     2461 ctccatcatg aagtgtgacg tggacatccg caaagacctg tacgccaaca cagtgctgtc
     2521 tggcggcacc accatgtacc ctggcattgc cgacaggatg cagaaggaga tcactgccct
     2581 ggcacccagc acaatgaaga tcaagatcat tgctcctcct gagcgcaagt actccgtgtg
     2641 gatcggcggc tccatcctgg cctcgctgtc caccttccag cagatgtgga tcagcaagca
     2701 ggagtatgac gagtccggcc cctccatcgt ccaccgcaaa tgcttctagg cggactatga
     2761 cttagttgcg ttacaccctt tcttgacaaa acctaacttg cgcagaaaac aagatgagat
     2821 tggcatggct ttatttgttt tttttgtttt gttttggttt tttttttttt tggcttgact
     2881 caggatttaa aaactggaac ggtgaaggtg acagcagtcg gttggagcga gcatccccca
     2941 aagttcacaa tgtggccgag gactttgatt gcacattgtt gtttttttaa tagtcattcc
     3001 aaatatgaga tgcattgtta caggaagtcc cttgccatcc taaaagccac cccacttctc
     3061 tctaaggaga atggcccagt cctctcccaa gtccacacag gggaggtgat agcattgctt
     3121 ggatccacta gtccagtgtg gtggaattcg ggccctatat atcgatccta aggtacctaa
     3181 ttgcctagaa aacatgagga tcacccatgt ctgcaggtcg actccagaaa acatgaggat
     3241 cacccatgtc tgcagtattc ccgggttcat tagatcctaa ggtacctaat tgcctagaaa
     3301 acatgaggat cacccatgtc tgcaggtcga ctctagaaaa catgaggatc acccatgtct
     3361 gcagtattcc cgggttcatt agatcctaag gtacctaatt gcctagaaaa catgaggatc
     3421 acccatgtct gcaggtcgac tccagaaaac atgaggatca cccatgtctg cagtattccc
     3481 gggttcatta gatcctaagg tacctaattg cctagaaaac atgaggatca cccatgtctg
     3541 caggtcgact ctagaaaaca tgaggatcac ccatgtctgc agtattcccg ggttcattag
     3601 atcctaaggt acctaattgc ctagaaaaca tgaggatcac ccatgtctgc aggtcgactc
     3661 tagaaaacat gaggatcacc catgtctgca gtattcccgg gttcattaga tcctaaggta
     3721 cctaattgcc tagaaaacat gaggatcacc catgtctgca ggtcgactcc agaaaacatg
     3781 aggatcaccc atgtctgcag tattcccggg ttcattagat ctgcgcgcga tcggatcagg
     3841 gccctatata tcgatcctaa ggtaccgaat tgcctagaaa acatgaggat cacccatgtc
     3901 tgcaggtcga ctccagaaaa catgaggatc acccatgtct gcagtattcc cgggttcatt
     3961 agatcctaag gtacctaatt gcctagaaaa catgaggatc acccatgtct gcaggtcgac
     4021 tctagaaaac atgaggatca cccatgtctg cagtattccc gggttcatta gatcctaagg
     4081 tacctaattg cctagaaaac atgaggatca cccatgtctg caggtcgact ccagaaaaca
     4141 tgaggatcac ccatgtctgc agtattcccg ggttcattag atcctaaggt acctaattgc
     4201 ctagaaaaca tgaggatcac ccatgtctgc aggtcgactc tagaaaacat gaggatcacc
     4261 catgtctgca gtattcccgg gttcattaga tcctaaggta cctaattgcc tagaaaacat
     4321 gaggatcacc catgtctgca ggtcgactct agaaaacatg aggatcaccc atgtctgcag
     4381 tattcccggg ttcattagat cctaaggtac ctaattgcct agaaaacatg aggatcaccc
     4441 atgtctgcag gtcgactcca gaaaacatga ggatcaccca tgtctgcagt attcccgggt
     4501 tcattagatc tgcgcgcgat cggatccgag ctcggtacca agcttctcga gtctagaggg
     4561 cccgtttaaa cccgctgatc agcctcgact gtgccttcta gttgccagcc atctgttgtt
     4621 tgcccctccc ccgtgccttc cttgaccctg gaaggtgcca ctcccactgt cctttcctaa
     4681 taaaatgagg aaattgcatc gcattgtctg agtaggtgtc attctattct ggggggtggg
     4741 gtggggcagg acagcaaggg ggaggattgg gaagacaata gcaggcatgc tggggatgcg
     4801 gtgggctcta tggcttctga ggcggaaaga accagctggg gctctagggg gtatccccac
     4861 gcgccctgta gcggcgcatt aagcgcggcg ggtgtggtgg ttacgcgcag cgtgaccgct
     4921 acacttgcca gcgccctagc gcccgctcct ttcgctttct tcccttcctt tctcgccacg
     4981 ttcgccggct ttccccgtca agctctaaat cgggggctcc ctttagggtt ccgatttagt
     5041 gctttacggc acctcgaccc caaaaaactt gattagggtg atggttcacg tagtgggcca
     5101 tcgccctgat agacggtttt tcgccctttg acgttggagt ccacgttctt taatagtgga
     5161 ctcttgttcc aaactggaac aacactcaac cctatctcgg tctattcttt tgatttataa
     5221 gggattttgc cgatttcggc ctattggtta aaaaatgagc tgatttaaca aaaatttaac
     5281 gcgaattaat tctgtggaat gtgtgtcagt tagggtgtgg aaagtcccca ggctccccag
     5341 caggcagaag tatgcaaagc atgcatctca attagtcagc aaccaggtgt ggaaagtccc
     5401 caggctcccc agcaggcaga agtatgcaaa gcatgcatct caattagtca gcaaccatag
     5461 tcccgcccct aactccgccc atcccgcccc taactccgcc cagttccgcc cattctccgc
     5521 cccatggctg actaattttt tttatttatg cagaggccga ggccgcctct gcctctgagc
     5581 tattccagaa gtagtgagga ggcttttttg gaggcctagg cttttgcaaa aagctcccaa
     5641 ccatgaccga gtacaagccc acggtgcgcc tcgccacccg cgacgacgtc cccagggccg
     5701 tacgcaccct cgccgccgcg ttcgccgact accccgccac gcgccacacc gtcgatccgg
     5761 accgccacat cgagcgggtc accgagctgc aagaactctt cctcacgcgc gtcgggctcg
     5821 acatcggcaa ggtgtgggtc gcggacgacg gcgccgcggt ggcggtctgg accacgccgg
     5881 agagcgtcga agcgggggcg gtgttcgccg agatcggccc gcgcatggcc gagttgagcg
     5941 gttcccggct ggccgcgcag caacagatgg aaggcctcct ggcgccgcac cggcccaagg
     6001 agcccgcgtg gttcctggcc accgtcggcg tctcgcccga ccaccagggc aagggtctgg
     6061 gcagcgccgt cgtgctcccc ggagtggagg cggccgagcg cgccggggtg cccgccttcc
     6121 tggagacctc cgcgccccgc aacctcccct tctacgagcg gctcggcttc accgtcaccg
     6181 ccgacgtcga ggtgcccgaa ggaccgcgca cctggtgcat gacccgcaag cccggtgcct
     6241 gagcgggact ctggggttcg aaatgaccga ccaagcgacg cccaacctgc catcacgaga
     6301 tttcgattcc accgccgcct tctatgaaag gttgggcttc ggaatcgttt tccgggacgc
     6361 cggctggatg atcctccagc gcggggatct catgctggag ttcttcgccc accccaactt
     6421 gtttattgca gcttataatg gttacaaata aagcaatagc atcacaaatt tcacaaataa
     6481 agcatttttt tcactgcatt ctagttgtgg tttgtccaaa ctcatcaatg tatcttatca
     6541 tgtctgtata ccgtcgacct ctagctagag cttggcgtaa tcatggtcat agctgtttcc
     6601 tgtgtgaaat tgttatccgc tcacaattcc acacaacata cgagccggaa gcataaagtg
     6661 taaagcctgg ggtgcctaat gagtgagcta actcacatta attgcgttgc gctcactgcc
     6721 cgctttccag tcgggaaacc tgtcgtgcca gctgcattaa tgaatcggcc aacgcgcggg
     6781 gagaggcggt ttgcgtattg ggcgctcttc cgcttcctcg ctcactgact cgctgcgctc
     6841 ggtcgttcgg ctgcggcgag cggtatcagc tcactcaaag gcggtaatac ggttatccac
     6901 agaatcaggg gataacgcag gaaagaacat gtgagcaaaa ggccagcaaa aggccaggaa
     6961 ccgtaaaaag gccgcgttgc tggcgttttt ccataggctc cgcccccctg acgagcatca
     7021 caaaaatcga cgctcaagtc agaggtggcg aaacccgaca ggactataaa gataccaggc
     7081 gtttccccct ggaagctccc tcgtgcgctc tcctgttccg accctgccgc ttaccggata
     7141 cctgtccgcc tttctccctt cgggaagcgt ggcgctttct catagctcac gctgtaggta
     7201 tctcagttcg gtgtaggtcg ttcgctccaa gctgggctgt gtgcacgaac cccccgttca
     7261 gcccgaccgc tgcgccttat ccggtaacta tcgtcttgag tccaacccgg taagacacga
     7321 cttatcgcca ctggcagcag ccactggtaa caggattagc agagcgaggt atgtaggcgg
     7381 tgctacagag ttcttgaagt ggtggcctaa ctacggctac actagaagaa cagtatttgg
     7441 tatctgcgct ctgctgaagc cagttacctt cggaaaaaga gttggtagct cttgatccgg
     7501 caaacaaacc accgctggta gcggtggttt ttttgtttgc aagcagcaga ttacgcgcag
     7561 aaaaaaagga tctcaagaag atcctttgat cttttctacg gggtctgacg ctcagtggaa
     7621 cgaaaactca cgttaaggga ttttggtcat gagattatca aaaaggatct tcacctagat
     7681 ccttttaaat taaaaatgaa gttttaaatc aatctaaagt atatatgagt aaacttggtc
     7741 tgacagttac caatgcttaa tcagtgaggc acctatctca gcgatctgtc tatttcgttc
     7801 atccatagtt gcctgactcc ccgtcgtgta gataactacg atacgggagg gcttaccatc
     7861 tggccccagt gctgcaatga taccgcgaga cccacgctca ccggctccag atttatcagc
     7921 aataaaccag ccagccggaa gggccgagcg cagaagtggt cctgcaactt tatccgcctc
     7981 catccagtct attaattgtt gccgggaagc tagagtaagt agttcgccag ttaatagttt
     8041 gcgcaacgtt gttgccattg ctacaggcat cgtggtgtca cgctcgtcgt ttggtatggc
     8101 ttcattcagc tccggttccc aacgatcaag gcgagttaca tgatccccca tgttgtgcaa
     8161 aaaagcggtt agctccttcg gtcctccgat cgttgtcaga agtaagttgg ccgcagtgtt
     8221 atcactcatg gttatggcag cactgcataa ttctcttact gtcatgccat ccgtaagatg
     8281 cttttctgtg actggtgagt actcaaccaa gtcattctga gaatagtgta tgcggcgacc
     8341 gagttgctct tgcccggcgt caatacggga taataccgcg ccacatagca gaactttaaa
     8401 agtgctcatc attggaaaac gttcttcggg gcgaaaactc tcaaggatct taccgctgtt
     8461 gagatccagt tcgatgtaac ccactcgtgc acccaactga tcttcagcat cttttacttt
     8521 caccagcgtt tctgggtgag caaaaacagg aaggcaaaat gccgcaaaaa agggaataag
     8581 ggcgacacgg aaatgttgaa tactcatact cttccttttt caatattatt gaagcattta
     8641 tcagggttat tgtctcatga gcggatacat atttgaatgt atttagaaaa ataaacaaat
     8701 aggggttccg cgcacatttc cccgaaaagt gccacctgac gtc
//
