LOCUS       sequence-410737-       11843 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  telomere specific nuclease to create double-strand breaks at 
            telomeres.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11843)
  AUTHORS   Dilley RL, Verma P, Cho NW, Winters HD, Wondisford AR, Greenberg RA
  TITLE     Break-induced telomere synthesis underlies alternative telomere 
            maintenance.
  JOURNAL   Nature. 2016 Nov 3;539(7627):54-58. doi: 10.1038/nature20099. Epub 
            2016 Oct 19.
  PUBMED    27760120
REFERENCE   2  (bases 1 to 11843)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/nature20099"; journalName: "Nature"; date: "2016-11-3- 3"; 
            volume: "539"; issue: "7627"; pages: "54-58"
COMMENT     Sequence Label: pLenti CMV TRE3G Puro FLAG-DD-ER-mCherry-TRF1-...
FEATURES             Location/Qualifiers
     source          1..11843
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(93..112)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(94..112)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(122..139)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(122..138)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(131..153)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      280..735
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(367..386)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     577..598
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        761..865
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(866..934,935..1726)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(1084..1103)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      1897..2485
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     2386..2405
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     2639..2656
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    2773..2794
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(2809..2814,2815..2832,2833..2839)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    2847..2863
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     2852..2874
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     2871..2887
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     2871..2887
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     2906..2926
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        2908..2926
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        2954..3180
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             3181..3361
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    3408..3533
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    4026..4259
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             4444..4488
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    4755..4872
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        4899..5277
                     /label=TRE3G promoter
                     /note="3rd-generation Tet-responsive promoter that can be 
                     activated by binding of Tet-On(R) 3G"
     protein_bind    4907..4925
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    4943..4961
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    4979..4997
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    5015..5033
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    5051..5069
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    5087..5105
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    5123..5141
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     primer_bind     5186..5210
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     protein_bind    5323..5347
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             5371..5436
                     /codon_start=1
                     /product="three tandem FLAG(R) epitope tags, followed by an
                     enterokinase cleavage site"
                     /label=3xFLAG
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
     CDS             5446..5766
                     /codon_start=1
                     /product="destabilization domain that can be stabilized by 
                     Shield1 in the ProteoTuner(TM) system"
                     /label=DD
                     /note="L106P mutant of FKBP12"
                     /translation="GVQVETISPGDGRTFPKRGQTCVVHYTGMLEDGKKVDSSRDRNKP
                     FKFMLGKQEVIRGWEEGVAQMSVGQRAKLTISPDYAYGATGHPGIIPPHATLVFDVELL
                     KPE"
     CDS             5818..6708
                     /codon_start=1
                     /gene="ESR1"
                     /product="estrogen nuclear receptor alpha ligand-binding 
                     domain"
                     /label=ER-LBD
                     /translation="KRSKKNSLALSLTADQMVSALLDAEPPILYSEYDPTRPFSEASMM
                     GLLTNLADRELVHMINWAKRVPGFVDLTLHDQVHLLECAWLEILMIGLVWRSMEHPVKL
                     LFAPNLLLDRNQGKCVEGMVEIFDMLLATSSRFRMMNLQGEEFVCLKSIILLNSGVYTF
                     LSSTLKSLEEKDHIHRVLDKITDTLIHLMAKAGLTLQQQHQRLAQLLLILSHIRHMSNK
                     GMEHLYSMKCKNVVPLYDLLLEAADAHRLHAPTSRGGASVEETDQSHLATAGSTSSHSL
                     QKYYITGEAEGFPATV"
     CDS             6715..7422
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /label=mCherry
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     primer_bind     complement(6860..6878)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     7128..7147
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     CDS             8791..9378
                     /codon_start=1
                     /product="nonspecific DNA cleavage domain of the FokI 
                     endonuclease (Li et al., 1992)"
                     /label=FokI cleavage domain
                     /note="must dimerize to be catalytically active"
                     /translation="QLVKSELEEKKSELRHKLKYVPHEYIELIEIARNSTQDRILEMKV
                     MEFFMKVYGYRGKHLGGSRKPDGAIYTVGSPIDYGVIVDTKAYSGGYNLPIGQADEMQR
                     YVEENQTRNKHINPNEWWKVYPSSVTEFKFLFVSGHFKGNYKAQLTRLNHITNCNGAVL
                     SVEELLIGGEMIKAGTLTLEEVRRKFNNGEINF"
     protein_bind    complement(9401..9425)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     misc_feature    9456..10044
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(9509..9529)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(9927..9938)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     promoter        10058..10557
                     /label=PGK promoter
                     /note="mouse phosphoglycerate kinase 1 promoter"
     primer_bind     complement(10093..10116)
                     /label=MSCV-rev
                     /note="Murine stem cell virus, reverse primer"
     primer_bind     10490..10509
                     /label=mPGK-F
                     /note="Mouse PGK promoter, forward primer"
     CDS             10578..11177
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(10578..10597)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     11074..11094
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     LTR             11315..11548
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    11620..11741
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(11657..11676)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     11711..11730
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      join(11781..11843,1..73)
                     /label=SV40 ori
                     /note="SV40 origin of replication"
ORIGIN
        1 atttatgcag aggccgaggc cgcctcggcc tctgagctat tccagaagta gtgaggaggc
       61 ttttttggag gcctagggac gtacccaatt cgccctatag tgagtcgtat tacgcgcgct
      121 cactggccgt cgttttacaa cgtcgtgact gggaaaaccc tggcgttacc caacttaatc
      181 gccttgcagc acatccccct ttcgccagct ggcgtaatag cgaagaggcc cgcaccgatc
      241 gcccttccca acagttgcgc agcctgaatg gcgaatggga cgcgccctgt agcggcgcat
      301 taagcgcggc gggtgtggtg gttacgcgca gcgtgaccgc tacacttgcc agcgccctag
      361 cgcccgctcc tttcgctttc ttcccttcct ttctcgccac gttcgccggc tttccccgtc
      421 aagctctaaa tcgggggctc cctttagggt tccgatttag tgctttacgg cacctcgacc
      481 ccaaaaaact tgattagggt gatggttcac gtagtgggcc atcgccctga tagacggttt
      541 ttcgcccttt gacgttggag tccacgttct ttaatagtgg actcttgttc caaactggaa
      601 caacactcaa ccctatctcg gtctattctt ttgatttata agggattttg ccgatttcgg
      661 cctattggtt aaaaaatgag ctgatttaac aaaaatttaa cgcgaatttt aacaaaatat
      721 taacgcttac aatttaggtg gcacttttcg gggaaatgtg cgcggaaccc ctatttgttt
      781 atttttctaa atacattcaa atatgtatcc gctcatgaga caataaccct gataaatgct
      841 tcaataatat tgaaaaagga agagtatgag tattcaacat ttccgtgtcg cccttattcc
      901 cttttttgcg gcattttgcc ttcctgtttt tgctcaccca gaaacgctgg tgaaagtaaa
      961 agatgctgaa gatcagttgg gtgcacgagt gggttacatc gaactggatc tcaacagcgg
     1021 taagatcctt gagagttttc gccccgaaga acgttttcca atgatgagca cttttaaagt
     1081 tctgctatgt ggcgcggtat tatcccgtat tgacgccggg caagagcaac tcggtcgccg
     1141 catacactat tctcagaatg acttggttga gtactcacca gtcacagaaa agcatcttac
     1201 ggatggcatg acagtaagag aattatgcag tgctgccata accatgagtg ataacactgc
     1261 ggccaactta cttctgacaa cgatcggagg accgaaggag ctaaccgctt ttttgcacaa
     1321 catgggggat catgtaactc gccttgatcg ttgggaaccg gagctgaatg aagccatacc
     1381 aaacgacgag cgtgacacca cgatgcctgt agcaatggca acaacgttgc gcaaactatt
     1441 aactggcgaa ctacttactc tagcttcccg gcaacaatta atagactgga tggaggcgga
     1501 taaagttgca ggaccacttc tgcgctcggc ccttccggct ggctggttta ttgctgataa
     1561 atctggagcc ggtgagcgtg ggtctcgcgg tatcattgca gcactggggc cagatggtaa
     1621 gccctcccgt atcgtagtta tctacacgac ggggagtcag gcaactatgg atgaacgaaa
     1681 tagacagatc gctgagatag gtgcctcact gattaagcat tggtaactgt cagaccaagt
     1741 ttactcatat atactttaga ttgatttaaa acttcatttt taatttaaaa ggatctaggt
     1801 gaagatcctt tttgataatc tcatgaccaa aatcccttaa cgtgagtttt cgttccactg
     1861 agcgtcagac cccgtagaaa agatcaaagg atcttcttga gatccttttt ttctgcgcgt
     1921 aatctgctgc ttgcaaacaa aaaaaccacc gctaccagcg gtggtttgtt tgccggatca
     1981 agagctacca actctttttc cgaaggtaac tggcttcagc agagcgcaga taccaaatac
     2041 tgttcttcta gtgtagccgt agttaggcca ccacttcaag aactctgtag caccgcctac
     2101 atacctcgct ctgctaatcc tgttaccagt ggctgctgcc agtggcgata agtcgtgtct
     2161 taccgggttg gactcaagac gatagttacc ggataaggcg cagcggtcgg gctgaacggg
     2221 gggttcgtgc acacagccca gcttggagcg aacgacctac accgaactga gatacctaca
     2281 gcgtgagcta tgagaaagcg ccacgcttcc cgaagggaga aaggcggaca ggtatccggt
     2341 aagcggcagg gtcggaacag gagagcgcac gagggagctt ccagggggaa acgcctggta
     2401 tctttatagt cctgtcgggt ttcgccacct ctgacttgag cgtcgatttt tgtgatgctc
     2461 gtcagggggg cggagcctat ggaaaaacgc cagcaacgcg gcctttttac ggttcctggc
     2521 cttttgctgg ccttttgctc acatgttctt tcctgcgtta tcccctgatt ctgtggataa
     2581 ccgtattacc gcctttgagt gagctgatac cgctcgccgc agccgaacga ccgagcgcag
     2641 cgagtcagtg agcgaggaag cggaagagcg cccaatacgc aaaccgcctc tccccgcgcg
     2701 ttggccgatt cattaatgca gctggcacga caggtttccc gactggaaag cgggcagtga
     2761 gcgcaacgca attaatgtga gttagctcac tcattaggca ccccaggctt tacactttat
     2821 gcttccggct cgtatgttgt gtggaattgt gagcggataa caatttcaca caggaaacag
     2881 ctatgaccat gattacgcca agcgcgcaat taaccctcac taaagggaac aaaagctgga
     2941 gctgcaagct taatgtagtc ttatgcaata ctcttgtagt cttgcaacat ggtaacgatg
     3001 agttagcaac atgccttaca aggagagaaa aagcaccgtg catgccgatt ggtggaagta
     3061 aggtggtacg atcgtgcctt attaggaagg caacagacgg gtctgacatg gattggacga
     3121 accactgaat tgccgcattg cagagatatt gtatttaagt gcctagctcg atacataaac
     3181 gggtctctct ggttagacca gatctgagcc tgggagctct ctggctaact agggaaccca
     3241 ctgcttaagc ctcaataaag cttgccttga gtgcttcaag tagtgtgtgc ccgtctgttg
     3301 tgtgactctg gtaactagag atccctcaga cccttttagt cagtgtggaa aatctctagc
     3361 agtggcgccc gaacagggac ttgaaagcga aagggaaacc agaggagctc tctcgacgca
     3421 ggactcggct tgctgaagcg cgcacggcaa gaggcgaggg gcggcgactg gtgagtacgc
     3481 caaaaatttt gactagcgga ggctagaagg agagagatgg gtgcgagagc gtcagtatta
     3541 agcgggggag aattagatcg cgatgggaaa aaattcggtt aaggccaggg ggaaagaaaa
     3601 aatataaatt aaaacatata gtatgggcaa gcagggagct agaacgattc gcagttaatc
     3661 ctggcctgtt agaaacatca gaaggctgta gacaaatact gggacagcta caaccatccc
     3721 ttcagacagg atcagaagaa cttagatcat tatataatac agtagcaacc ctctattgtg
     3781 tgcatcaaag gatagagata aaagacacca aggaagcttt agacaagata gaggaagagc
     3841 aaaacaaaag taagaccacc gcacagcaag cggccgctga tcttcagacc tggaggagga
     3901 gatatgaggg acaattggag aagtgaatta tataaatata aagtagtaaa aattgaacca
     3961 ttaggagtag cacccaccaa ggcaaagaga agagtggtgc agagagaaaa aagagcagtg
     4021 ggaataggag ctttgttcct tgggttcttg ggagcagcag gaagcactat gggcgcagcg
     4081 tcaatgacgc tgacggtaca ggccagacaa ttattgtctg gtatagtgca gcagcagaac
     4141 aatttgctga gggctattga ggcgcaacag catctgttgc aactcacagt ctggggcatc
     4201 aagcagctcc aggcaagaat cctggctgtg gaaagatacc taaaggatca acagctcctg
     4261 gggatttggg gttgctctgg aaaactcatt tgcaccactg ctgtgccttg gaatgctagt
     4321 tggagtaata aatctctgga acagatttgg aatcacacga cctggatgga gtgggacaga
     4381 gaaattaaca attacacaag cttaatacac tccttaattg aagaatcgca aaaccagcaa
     4441 gaaaagaatg aacaagaatt attggaatta gataaatggg caagtttgtg gaattggttt
     4501 aacataacaa attggctgtg gtatataaaa ttattcataa tgatagtagg aggcttggta
     4561 ggtttaagaa tagtttttgc tgtactttct atagtgaata gagttaggca gggatattca
     4621 ccattatcgt ttcagaccca cctcccaacc ccgaggggac ccgacaggcc cgaaggaata
     4681 gaagaagaag gtggagagag agacagagac agatccattc gattagtgaa cggatctcga
     4741 cggtatcggt taacttttaa aagaaaaggg gggattgggg ggtacagtgc aggggaaaga
     4801 atagtagaca taatagcaac agacatacaa actaaagaat tacaaaaaca aattacaaaa
     4861 attcaaaatt ttatcgatca cgagactagc ctcgagttgg ctttactccc tatcagtgat
     4921 agagaacgta tgaagagttt actccctatc agtgatagag aacgtatgca gactttactc
     4981 cctatcagtg atagagaacg tataaggagt ttactcccta tcagtgatag agaacgtatg
     5041 accagtttac tccctatcag tgatagagaa cgtatctaca gtttactccc tatcagtgat
     5101 agagaacgta tatccagttt actccctatc agtgatagag aacgtataag ctttaggcgt
     5161 gtacggtggg cgcctataaa agcagagctc gtttagtgaa ccgtcagatc gcctggagca
     5221 attccacaac acttttgtct tataccaact ttccgtacca cttcctaccc tcgtaaaccg
     5281 gttcgtcgac tagtccagtg tggtggaatt ctgcagatat caacaagttt gtacaaaaaa
     5341 gcaggctccg cggccgcccc cttcaccatg gactacaaag accatgacgg tgattataaa
     5401 gatcatgaca tcgattacaa ggatgacgat gacaagcttg gaagcggagt gcaggtggaa
     5461 accatctccc caggagacgg gcgcaccttc cccaagcgcg gccagacctg tgtggtgcac
     5521 tacaccggga tgcttgaaga tggaaagaaa gtcgattcct cccgggacag aaacaagccc
     5581 tttaagttta tgctaggcaa gcaggaggtg atccgaggct gggaagaagg ggttgcccag
     5641 atgagtgtgg gtcagagagc caaactgact atatctccag attatgccta tggtgccact
     5701 gggcacccag gcatcatccc accacatgcc actctcgtct tcgatgtgga gcttctaaaa
     5761 ccggaaaccg gtgaattcat gagagctgcc aacctttggc caagcccgct catgatcaaa
     5821 cgctctaaga agaacagcct ggccttgtcc ctgacggccg accagatggt cagtgccttg
     5881 ttggatgctg agccccccat actctattcc gagtatgatc ctaccagacc cttcagtgaa
     5941 gcttcgatga tgggcttact gaccaacctg gcagacaggg agctggttca catgatcaac
     6001 tgggcgaaga gggtgccagg ctttgtggat ttgaccctcc atgatcaggt ccaccttcta
     6061 gaatgtgcct ggctagagat cctgatgatt ggtctcgtct ggcgctccat ggagcaccca
     6121 gtgaagctac tgtttgctcc taacttgctc ttggacagga accagggaaa atgtgtagag
     6181 ggcatggtgg agatcttcga catgctgctg gctacatcat ctcggttccg catgatgaat
     6241 ctgcagggag aggagtttgt gtgcctcaaa tctattattt tgcttaattc tggagtgtac
     6301 acatttctgt ccagcaccct gaagtctctg gaagagaagg accatatcca ccgagtcctg
     6361 gacaagatca cagacacttt gatccacctg atggccaagg caggcctgac cctgcagcag
     6421 cagcaccagc ggctggccca gctcctcctc atcctctccc acatcaggca catgagtaac
     6481 aaaggcatgg agcatctgta cagcatgaag tgcaagaacg tggtgcccct ctatgacctg
     6541 ctgctggagg cggcggacgc ccaccgccta catgcgccca ctagccgtgg aggggcatcc
     6601 gtggaggaga cggaccaaag ccacttggcc actgcgggct ctacttcatc gcattccttg
     6661 caaaagtatt acatcacggg ggaggcagag ggtttccctg ccacggtcct cgacatggtg
     6721 agcaagggcg aggaggataa catggccatc atcaaggagt tcatgcgctt caaggtgcac
     6781 atggagggct ccgtgaacgg ccacgagttc gagatcgagg gcgagggcga gggccgcccc
     6841 tacgagggca cccagaccgc caagctgaag gtgaccaagg gtggccccct gcccttcgcc
     6901 tgggacatcc tgtcccctca gttcatgtac ggctccaagg cctacgtgaa gcaccccgcc
     6961 gacatccccg actacttgaa gctgtccttc cccgagggct tcaagtggga gcgcgtgatg
     7021 aacttcgagg acggcggcgt ggtgaccgtg acccaggact cctccctgca ggacggcgag
     7081 ttcatctaca aggtgaagct gcgcggcacc aacttcccct ccgacggccc cgtaatgcag
     7141 aagaagacca tgggctggga ggcctcctcc gagcggatgt accccgagga cggcgccctg
     7201 aagggcgaga tcaagcagag gctgaagctg aaggacggcg gccactacga cgctgaggtc
     7261 aagaccacct acaaggccaa gaagcccgtg cagctgcccg gcgcctacaa cgtcaacatc
     7321 aagttggaca tcacctccca caacgaggac tacaccatcg tggaacagta cgaacgcgcc
     7381 gagggccgcc actccaccgg cggcatggac gagctgtaca aggcggccgc taagcttgcg
     7441 gaggatgttt cctcagcggc cccgagcccg cggggctgtg cggatggtag ggatgccgac
     7501 cctactgagg agcagatggc agaaacagag agaaacgacg aggagcagtt cgaatgccag
     7561 gaactgctcg agtgccaggt gcaggtgggg gcccccgagg aggaggagga ggaggaggag
     7621 gacgcgggcc tggtggccga ggccgaggcc gtggctgccg gctggatgct cgatttcctc
     7681 tgcctctctc tttgccgagc tttccgcgac ggccgctccg aggacttccg caggacccgc
     7741 aacagcgcag aggctattat tcatggacta tccagtctaa cagcttgcca gttgagaacg
     7801 atatacatat gtcagttttt gacaagaatt gcagcaggaa aaacccttga tgcacagttt
     7861 gaaaatgatg aacgaattac acccttggaa tcagccctga tgatttgggg ttcaattgaa
     7921 aaggaacatg acaaacttca tgaagaaata cagaatttaa ttaaaattca ggctatagct
     7981 gtttgtatgg aaaatggcaa ctttaaagaa gcagaagaag tctttgaaag aatatttggt
     8041 gatccaaatt ctcatatgcc tttcaaaagc aaattgctta tgataatctc tcagaaagat
     8101 acatttcatt ccttttttca acacttcagc tacaaccaca tgatggagaa aattaagagt
     8161 tatgtgaatt atgtgctaag tgaaaaatca tcaacctttc taatgaaggc agcggcaaaa
     8221 gtagtagaaa gcaaaaggac aagaacaata acttctcaag ataaacctag tggtaatgat
     8281 gttgaaatgg aaactgaagc taatttggat acaagaaaaa gtgttagtga caaacagtct
     8341 gcggtaactg aatcctcaga gggtacagta tccttattga ggtctcacaa gaatcttttc
     8401 ttatctaagt tgcaacatgg aacccagcaa caagacctta ataagaaaga aagaagagta
     8461 ggaactcctc aaagtacaaa aaagaaaaaa gaaagcagaa gagccactga aagcagaata
     8521 cctgtttcaa agagtcagcc ggtaactcct gaaaaacatc gagctagaaa aagacaggca
     8581 tggctttggg aagaagacaa gaatttgaga tctggcgtga ggaaatatgg agagggaaac
     8641 tggtctaaaa tactgttgca ttataaattc aacaaccgga caagtgtcat gttaaaagac
     8701 agatggagga ccatgaagaa actaaaactg atttcctcag acagcgaaga cggtaccacg
     8761 acgataaatc tagatagtaa gcttggatcc caactagtca aaagtgaact ggaggagaag
     8821 aaatctgaac ttcgtcataa attgaaatat gtgcctcatg aatatattga attaattgaa
     8881 attgccagaa attccactca ggatagaatt cttgaaatga aggtaatgga attttttatg
     8941 aaagtttatg gatatagagg taaacatttg ggtggatcaa ggaaaccgga cggagcaatt
     9001 tatactgtcg gatctcctat tgattacggt gtgatcgtgg atactaaagc ttatagcgga
     9061 ggttataatc tgccaattgg ccaagcagat gaaatgcaac gatatgtcga agaaaatcaa
     9121 acacgaaaca aacatatcaa ccctaatgaa tggtggaaag tctatccatc ttctgtaacg
     9181 gaatttaagt ttttatttgt gagtggtcac tttaaaggaa actacaaagc tcagcttaca
     9241 cgattaaatc atatcactaa ttgtaatgga gctgttctta gtgtagaaga gcttttaatt
     9301 ggtggagaaa tgattaaagc cggcacatta accttagagg aagtgagacg gaaatttaat
     9361 aacggcgaga taaactttgg ttaaaagggt gggcgcgccg acccagcttt cttgtacaaa
     9421 gtggttgata tccagcacag tggcggccgc tcgacaatca acctctggat tacaaaattt
     9481 gtgaaagatt gactggtatt cttaactatg ttgctccttt tacgctatgt ggatacgctg
     9541 ctttaatgcc tttgtatcat gctattgctt cccgtatggc tttcattttc tcctccttgt
     9601 ataaatcctg gttgctgtct ctttatgagg agttgtggcc cgttgtcagg caacgtggcg
     9661 tggtgtgcac tgtgtttgct gacgcaaccc ccactggttg gggcattgcc accacctgtc
     9721 agctcctttc cgggactttc gctttccccc tccctattgc cacggcggaa ctcatcgccg
     9781 cctgccttgc ccgctgctgg acaggggctc ggctgttggg cactgacaat tccgtggtgt
     9841 tgtcggggaa gctgacgtcc tttccatggc tgctcgcctg tgttgccacc tggattctgc
     9901 gcgggacgtc cttctgctac gtcccttcgg ccctcaatcc agcggacctt ccttcccgcg
     9961 gcctgctgcc ggctctgcgg cctcttccgc gtcttcgcct tcgccctcag acgagtcgga
    10021 tctccctttg ggccgcctcc ccgcctggaa ttctaccggg taggggaggc gcttttccca
    10081 aggcagtctg gagcatgcgc tttagcagcc ccgctgggca cttggcgcta cacaagtggc
    10141 ctctggcctc gcacacattc cacatccacc ggtaggcgcc aaccggctcc gttctttggt
    10201 ggccccttcg cgccaccttc tactcctccc ctagtcagga agttcccccc cgccccgcag
    10261 ctcgcgtcgt gcaggacgtg acaaatggaa gtagcacgtc tcactagtct cgtgcagatg
    10321 gacagcaccg ctgagcaatg gaagcgggta ggcctttggg gcagcggcca atagcagctt
    10381 tgctccttcg ctttctgggc tcagaggctg ggaaggggtg ggtccggggg cgggctcagg
    10441 ggcgggctca ggggcggggc gggcgcccga aggtcctccg gaggcccggc attctgcacg
    10501 cttcaaaagc gcacgtctgc cgcgctgttc tcctcttcct catctccggg cctttcgacc
    10561 tgcagcccaa gcttaccatg accgagtaca agcccacggt gcgcctcgcc acccgcgacg
    10621 acgtccccag ggccgtacgc accctcgccg ccgcgttcgc cgactacccc gccacgcgcc
    10681 acaccgtcga tccggaccgc cacatcgagc gggtcaccga gctgcaagaa ctcttcctca
    10741 cgcgcgtcgg gctcgacatc ggcaaggtgt gggtcgcgga cgacggcgcc gcggtggcgg
    10801 tctggaccac gccggagagc gtcgaagcgg gggcggtgtt cgccgagatc ggcccgcgca
    10861 tggccgagtt gagcggttcc cggctggccg cgcagcaaca gatggaaggc ctcctggcgc
    10921 cgcaccggcc caaggagccc gcgtggttcc tggccaccgt cggcgtctcg cccgaccacc
    10981 agggcaaggg tctgggcagc gccgtcgtgc tccccggagt ggaggcggcc gagcgcgccg
    11041 gggtgcccgc cttcctggag acctccgcgc cccgcaacct ccccttctac gagcggctcg
    11101 gcttcaccgt caccgccgac gtcgaggtgc ccgaaggacc gcgcacctgg tgcatgaccc
    11161 gcaagcccgg tgcctgacgc ccgccccacg acccgcagcg cccgaccgaa aggagcgcac
    11221 gaccccatgc atctcgaggg cccggtacct ttaagaccaa tgacttacaa ggcagctgta
    11281 gatcttagcc actttttaaa agaaaagggg ggactggaag ggctagctca ctcccaacga
    11341 agacaagatc tgctttttgc ttgtactggg tctctctggt tagaccagat ctgagcctgg
    11401 gagctctctg gctaactagg gaacccactg cttaagcctc aataaagctt gccttgagtg
    11461 cttcaagtag tgtgtgcccg tctgttgtgt gactctggta actagagatc cctcagaccc
    11521 ttttagtcag tgtggaaaat ctctagcagt agtagttcat gtcatcttat tattcagtat
    11581 ttataacttg caaagaaatg aatatcagag agtgagagga acttgtttat tgcagcttat
    11641 aatggttaca aataaagcaa tagcatcaca aatttcacaa ataaagcatt tttttcactg
    11701 cattctagtt gtggtttgtc caaactcatc aatgtatctt atcatgtctg gctctagcta
    11761 tcccgcccct aactccgccc atcccgcccc taactccgcc cagttccgcc cattctccgc
    11821 cccatggctg actaattttt ttt
//
