LOCUS       sequence-410739-       13968 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Module for the expression of geneticin resistance, Nluc under PgpdA 
            promoter and luciferase under a synthetic promoter containing two 
            copies of the target sequence for gRNA1 (2xLuc)..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 13968)
  AUTHORS   Moreno-Gimenez E, Gandia M, Saez Z, Manzanares P, Yenush L, Orzaez 
            D, Marcos JF, Garrigues S
  TITLE     FungalBraid 2.0: expanding the synthetic biology toolbox for the 
            biotechnological exploitation of filamentous fungi.
  JOURNAL   Front Bioeng Biotechnol. 2023 Aug 7;11:1222812. doi: 
            10.3389/fbioe.2023.1222812. eCollection 2023.
  PUBMED    37609115
REFERENCE   2  (bases 1 to 13968)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Front 
            Bioeng Biotechnol."; date: "2023-08-7"; volume: "11:1222812. doi"; 
            pages: " 10.3389/fbioe.2023.1222812. eCollection 2023"
COMMENT     Sequence Label: FB399
FEATURES             Location/Qualifiers
     source          1..13968
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             complement(286..1080)
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(397..416)
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     primer_bind     1007..1026
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     CDS             5360..7012
                     /codon_start=1
                     /gene="luc+"
                     /product="firefly luciferase"
                     /label=luciferase
                     /note="enhanced luc+ version of the luciferase gene"
                     /translation="MEDAKNIKKGPAPFYPLEDGTAGEQLHKAMKRYALVPGTIAFTDA
                     HIEVDITYAEYFEMSVRLAEAMKRYGLNTNHRIVVCSENSLQFFMPVLGALFIGVAVAP
                     ANDIYNERELLNSMGISQPTVVFVSKKGLQKILNVQKKLPIIQKIIIMDSKTDYQGFQS
                     MYTFVTSHLPPGFNEYDFVPESFDRDKTIALIMNSSGSTGLPKGVALPHRTACVRFSHA
                     RDPIFGNQIIPDTAILSVVPFHHGFGMFTTLGYLICGFRVVLMYRFEEELFLRSLQDYK
                     IQSALLVPTLFSFFAKSTLIDKYDLSNLHEIASGGAPLSKEVGEAVAKRFHLPGIRQGY
                     GLTETTSAILITPEGDDKPGAVGKVVPFFEAKVVDLDTGKTLGVNQRGELCVRGPMIMS
                     GYVNNPEATNALIDKDGWLHSGDIAYWDEDEHFFIVDRLKSLIKYKGYQVAPAELESIL
                     LQHPNIFDAGVAGLPDDDAGELPAAVVVLEHGKTMTEKEIVDYVASQVTTAKKLRGGVV
                     FVDEVPKGLTGKLDARKIREILIKAKKGGKIAV"
     primer_bind     complement(5426..5444)
                     /label=LucNrev
                     /note="Firefly luciferase, reverse primer"
     misc_feature    7286..7310
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     CDS             8611..9240
                     /codon_start=1
                     /product="stability protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 StaA
                     /translation="MKVIAVLNQKGGSGKTTIATHLARALQLAGADVLLVDSDPQGSAR
                     DWAAVREDQPLTVVGIDRPTIDRDVKAIGRRDFVVIDGAPQAADLAVSAIKAADFVLIP
                     VQPSPYDIWATADLVELVKQRIEVTDGRLQAAFVVSRAIKGTRIGGEVAEALAGYELPI
                     LESRITQRVSYPGTAAAGTTVLESEPEGDAAREVQALAAEIKSKLI"
     CDS             9669..10742
                     /codon_start=1
                     /product="replication protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 RepA
                     /translation="MSGRKPSGPVQIGAALGDDLVEKLKAAQAAQRQRIEAEARPGESW
                     QAAADRIRKESRQPPAAGAPSIRKPPKGDEQPDFFVPMLYDVGTRDSRSIMDVAVFRLS
                     KRDRRAGEVIRYELPDGHVEVSAGPAGMASVWDYDLVLMAVSHLTESMNRYREGKGDKP
                     GRVFRPHVADVLKFCRRADGGKQKDDLVETCIRLNTTHVAMQRTKKAKNGRLVTVSEGE
                     ALISRYKIVKSETGRPEYIEIELADWMYREITEGKNPDVLTVHPDYFLIDPGIGRFLYR
                     LARRAAGKAEARWLFKTIYERSGSAGEFKKFCFTVRKLIGSNDLPEYDLKEEAGQAGPI
                     LVMRYRNLIEGEASAGS"
     rep_origin      10808..11002
                     /label=pVS1 oriV
                     /note="origin of replication for the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
     primer_bind     complement(11238..11260)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     misc_feature    11346..11486
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     11401..11420
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     complement(11501..11518)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(11672..12260)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(11752..11771)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(12348..13139)
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     misc_feature    13925..13949
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
ORIGIN
        1 gagagcgtca tcatgcaaca tgcatgtact gtctgatgta ttaagagtat aggggtcaac
       61 aactagagtg tctgacaggt atatctagca gtttgatttg accattcttt tattaaaaca
      121 ctgtaacccg caacgaagaa ctcctccgct tcaagtcaag aatgcgcata tgtgcgtaaa
      181 atcgacaagc ccggcggcat tccaccgagc agggtattgc ctttggcact ttacgtgcgc
      241 agaggagcct gaatgttgag tggaatgatg gtggaatgat gaagctcaga agaactcgtc
      301 aagaaggcga tagaaggcta tgcgctgcga atcgggagcg gcgataccgt aaagcacgag
      361 gaagcggtca gcccattcgc cgccaagctc ttcagcaata tcacgggtag ccaacgctat
      421 gtcctgatag cggtccgcca cacccagccg gccacagtcg atgaatccag aaaagcggcc
      481 attttccacc atgatattcg gcaagcaggc gtcgccgtgg gtcacgacga gatcctcgcc
      541 gtcgggcatc cgcgccttga gcctggcgaa cagttcggct ggcgcgagcc cctgatgctc
      601 ttcgtccaga tcatcctgat cgacaagacc ggcttccatc cgagtacgtg ctcgctcgat
      661 gcggtgtttc gcttggtggt cgaatgggca ggtagccgga tcaagcgtat gcagccgccg
      721 cattgcatca gccatgatgg atactttctc ggcaggagca aggtgagatg acaggagatc
      781 ctgccccggc acttcgccca atagcagcca gtcccttccc gcttcagtga caacgtcgag
      841 cacagctgcg caaggaacgc ccgtcgtggc cagccacgat agccgcgctg cctcgtcttg
      901 gagttcattc agggcaccgg acaggtcggt cttgacaaaa agaaccgggc gcccctgcgc
      961 tgacagccgg aacacggcgg catcagagca gccgattgtc tgttgtgccc agtcatagcc
     1021 gaatagcctc tccacccaag cggccggaga acctgcgtgc aatccatctt gttcaatcat
     1081 tttggatgct tgggtagaat aggtaagtca gattgaatct gaaataaagg gaggaagggc
     1141 gaacttaaga aggtatgacc gggtcgtcca cttaccttgc ttgacaaacg caccaagtta
     1201 tcgtgcacca agcagcagat gataataatg tcctcgttcc tgtctgctaa taagagtcac
     1261 acttcgagcg ccgccgctac tgctacaagt ggggctgatc tgaccagttg cctaaatgaa
     1321 ccatcttgtc aaacgacaca aattttgtgc tcaccgcctg gacgactaaa ccaaaatagg
     1381 cattcattgt tgacctccac tagctccagc caagcccaaa aaatgctcct tcaatatcag
     1441 ttctcctgac ggagtggcgc atgcggacag acggacggac gcagagagaa gggctgagta
     1501 ataagcgcca ctgcgccaga cagctctggc ggctctgagg tgcagtggat gattattaat
     1561 ccgggaccgg ccgcccctcc gccccgaagt ggaaaggctg gtgtgcccct cgttgaccaa
     1621 gaatctattg catcatcgga gaatatggag cttcatcgaa tcaccggcag taagcgaagg
     1681 agaatgtgaa gccaggggtg tatagccgtc ggcgaaatag catgccatta acctaggtac
     1741 agaagtccaa ttgcttccga tctggtaaaa gattcacgag atagtacctt ctccgaagta
     1801 ggtagagcga gtacccggcg cgtaagctcc ctaattggcc catccggcat ctgtagggcg
     1861 tccaaatatc gtgcctctcc tgctttgccc ggtgtatgaa accggaaagg ccgctcagga
     1921 gctggccagc ggcgcagacc gggaacacaa gctggcagtc gacccatccg gtgctctgca
     1981 ctcgacctgc tgaggtccct cagtccctgg taggcagctt tgccccgtct gtccgcccgg
     2041 tgtgtcggcg gggttgacaa ggtcgttgcg tcagtccaac atttgttgcc atattttcct
     2101 gctctcccca ccagctgctc ttttcttttc tctttctttt cccatcttca gtatattcat
     2161 cttcccatcc aagaaccttt aaaatggttt tcacccttga agatttcgtc ggcgactggc
     2221 gccaaaccgc cggatataac cttgaccagg tgctggagca gggaggagtc tcttccttgt
     2281 tccagaatct cggcgtttcc gttactccta tccagcgtat tgtgttgagc ggagagaacg
     2341 gtcttaagat tgacatccac gtcattatcc catacgaggg cctctctggc gatcaaatgg
     2401 gacagatcga gaaaattttc aaggtcgttt atccagtcga cgatcatcac tttaaagtta
     2461 tcctgcacta cggtactctt gtgatcgatg gcgtcacccc caacatgatt gactacttcg
     2521 gccgacccta cgagggtatc gctgtgttcg acggtaagaa gattactgtg accggtactt
     2581 tgtggaatgg aaacaagatt atcgatgagc gcctcatcaa ccctgatggc tccttgctgt
     2641 ttcgtgttac cattaacgga gttactggtt ggcgcctttg cgaacgtatt ctcgcataag
     2701 cttcatcatt ccaccatcat tccactcaac attcaggctc ctctgcgcac gtaaagtgcc
     2761 aaaggcaata ccctgctcgg tggaatgccg ccgggcttgt cgattttacg cacatatgcg
     2821 cattcttgac ttgaagcgga ggagttcttc gttgcgggtt acagtgtttt aataaaagaa
     2881 tggtcaaatc aaactgctag atatacctgt cagacactct agttgttgac ccctatactc
     2941 ttaatacatc agacagtaca tgcatgttgc atgatgacgc tctccgtcag gagtggctat
     3001 tgttgttatc atcaaaatct atattgatac tattatactc gatatgttat agactttatt
     3061 aaaaaaaact ttgtttgttt gcagaaagtg gatttttaat caaatttaat tgtgatgatt
     3121 acaatgtcaa ttggttatgc aagatattaa ttaatatgtt taagattttt cacttttaat
     3181 aagtaaaata aaatacgtca aaaaaatttc ctaaagataa tgccaaaaag gttaaattat
     3241 agaaattaaa ctaaaatata ttaaaagttg acttaaaaat gcaatgttga ccaccgacct
     3301 aggggaaaac aaatttaccg tatctgggat agaatctctt ctgtatcatc tcagataaag
     3361 attactacat aaacacactt tacagtttta cgacgcccaa aattcaattc ccggagatta
     3421 aaaaccctaa aacaaacact ggcgattcga ttttccctcc caattcaaat catctcttaa
     3481 tcttcttctt ctcactatcc tctcaaattc gccgccgatc ttccatcaat tttgatttct
     3541 cgcaccgaaa tttcgacttt tcatctccta tcgacgaaac cctaattttt cacactccag
     3601 aaatcgaact ttgacgacga tgttaatggc taaaccccgc tgtcaggagt ctcaatggta
     3661 actttactct ttatttaacc atacattttt ttttattttt ttcactttgt tcttcatcca
     3721 ctattgttct ttgttcatct tgaacaaaag ctccctcctt ctttgttctt catccaccat
     3781 tgttcttcat caatcatttc gctgtcagga gtgtttcatg tccaagcgaa aagccgctct
     3841 acggaatgga tctacgttac tgcctgcata aggagatcgg tgtagccaag gacgaaagcg
     3901 accctaggtt ctaaccgtcg actttggcgg aaaggtttca ctcaggaagc agacactgat
     3961 tgacacggtt tagcagaacg tttgaggact aggtcaaatt gagtggttta atatcggcat
     4021 gtctggcttt aaaattcagt atagtgcgct gatcggagat gaattaaaaa cacgagttcc
     4081 caaaaccagg cgggctcgcc acgtcggcta atcctggtac attatgtgaa caatgttctg
     4141 aagaaaattt gtgaaagaag gacgggtcat cgcctactat tagcaacaac ggtcggccac
     4201 accttccatt gtcgtggcca cgctcggatt acacggcaga ggtgcttgtg ttccgacagg
     4261 ctagcatatt atcctaaggc gttaccccaa tcgtttaccg tcggatttgc tatagcccct
     4321 gaacgctaca tgtacgaaac catgttatgt atgcactagg tcaacaatag gacatagcct
     4381 tgtagttaac acgtagcccg gtcgtataag tacagtagac ccttcgccgg catcctattt
     4441 attaagttat ttctacagca aaacgatcat atgcagatcc gcagtgggcg gtagacacac
     4501 gtccacccgg ctgctctgta acagggacta aagaggtgat gattatcgtg tgtgccccgt
     4561 tatggtcgtg ttcgatcaga gcgcccttgc gagcagtcgt atgctttctc gaattccgag
     4621 cggttaagcg tgacagtccc agcgaaccca caaaacgtga tcgcagtcca tgcgatcata
     4681 cgcaagaagg aaggtcccca tacaccgacg caccagttta cacgccgtat gcataaacga
     4741 gctgcacaaa cgagagtgtt tgaactggac ctctagttcc tctacaaaga acaccttgac
     4801 ctgttgcgaa gttgccttgc ctagatgcaa tgtcggacgt atcacttttg cctcaacgac
     4861 tgctgctttc gctgtaaccc aagacagaca acagtaaccg ccttttgaag gcaagtcctc
     4921 cgcctgtgac taactgtgcc aaatcgtctt ccaaacccct tatccaattt aactcaccaa
     4981 attattgcga tacagaccct aatttcacat catatgacac taattgcctc tgccaaaatt
     5041 ctgtcctcaa tgactccctc ttctctcacc aaccagtcag tcgtgaaagt catagtaccc
     5101 tgggtaccaa cttacccgtc ttctctcacc aaccagtccg ctggttcctg ggtgagctcg
     5161 agactcgtgg tgacagctct tcatacatag agcggccgcg tcgaactccc tgtcaccttt
     5221 cacacagagc tcatgatctg gtttataaag gcggcttcat gaccctcaat tccatatagt
     5281 atcactccca tcacagcatt tcgatatctt caaccacttt aaccttctcc agaggatcat
     5341 catctcaagc ccttcataaa tggaagacgc caaaaacata aagaaaggcc cggcgccatt
     5401 ctatccgctg gaagatggaa ccgctggaga gcaactgcat aaggctatga agagatacgc
     5461 cctggttcct ggaacaattg cttttacaga tgcacatatc gaggtggaca tcacttacgc
     5521 tgagtacttc gaaatgtccg ttcggttggc agaagctatg aaacgatatg ggctgaatac
     5581 aaatcacaga atcgtcgtat gcagtgaaaa ctctcttcaa ttctttatgc cggtgttggg
     5641 cgcgttattt atcggagttg cagttgcgcc cgcgaacgac atttataatg aacgtgaatt
     5701 gctcaacagt atgggcattt cgcagcctac cgtggtgttc gtttccaaaa aggggttgca
     5761 aaaaattttg aacgtgcaaa aaaagctccc aatcatccaa aaaattatta tcatggattc
     5821 taaaacggat taccagggat ttcagtcgat gtacacgttc gtcacatctc atctacctcc
     5881 cggttttaat gaatacgatt ttgtgccaga gtccttcgat agggacaaga caattgcact
     5941 gatcatgaac tcctctggat ctactggtct gcctaaaggt gtcgctctgc ctcatagaac
     6001 tgcctgcgtg agattctcgc atgccagaga tcctattttt ggcaatcaaa tcattccgga
     6061 tactgcgatt ttaagtgttg ttccattcca tcacggtttt ggaatgttta ctacactcgg
     6121 atatttgata tgtggatttc gagtcgtctt aatgtataga tttgaagaag agctgtttct
     6181 gaggagcctt caggattaca agattcaaag tgcgctgctg gtgccaaccc tattctcctt
     6241 cttcgccaaa agcactctga ttgacaaata cgatttatct aatttacacg aaattgcttc
     6301 tggtggcgct cccctctcta aggaagtcgg ggaagcggtt gccaagaggt tccatctgcc
     6361 aggtatcagg caaggatatg ggctcactga gactacatca gctattctga ttacacccga
     6421 gggggatgat aaaccgggcg cggtcggtaa agttgttcca ttttttgaag cgaaggttgt
     6481 ggatctggat accgggaaaa cgctgggcgt taatcaaaga ggcgaactgt gtgtgagagg
     6541 tcctatgatt atgtccggtt atgtaaacaa tccggaagcg accaacgcct tgattgacaa
     6601 ggatggatgg ctacattctg gagacatagc ttactgggac gaagacgaac acttcttcat
     6661 cgttgaccgc ctgaagtctc tgattaagta caaaggctat caggtggctc ccgctgaatt
     6721 ggaatccatc ttgctccaac accccaacat cttcgacgca ggtgtcgcag gtcttcccga
     6781 cgatgacgcc ggtgaacttc ccgccgccgt tgttgttttg gagcacggaa agacgatgac
     6841 ggaaaaagag atcgtggatt acgtcgccag tcaagtaaca accgcgaaaa agttgcgcgg
     6901 aggagttgtg tttgtggacg aagtaccgaa aggtcttacc ggaaaactcg acgcaagaaa
     6961 aatcagagag atcctcataa aggccaagaa gggcggaaag atcgccgtgt aatgagctta
     7021 cttaacgtta ctgaaatcat caaacagctt gacgaatctg gatataagat cgttggtgtc
     7081 gatgtcagct ccggagttga gacaaatggt gttcaggatc tcgataagat acgttcattt
     7141 gtccaagcag caaagagtgc cttctagtga tttaatagct ccatgtcaac aagaataaaa
     7201 cgcgtttcgg gtttacctct tccagataca gctcatctgc aatgcatcga ccgtctgaga
     7261 catgcgctgt catgagaccg gatcctgaca ggatatattg gcgggtaaac ctaagagaaa
     7321 agagcgttta ttagaataat cggatattta aaagggcgtg aaaaggttta tccgttcgtc
     7381 catttgtatg tgcatgccaa ccacagggtt cccctcggga tcaaagtact ttgatccaac
     7441 ccctccgctg ctatagtgca gtcggcttct gacgttcagt gcagccgtca tctgaaaacg
     7501 acatgtcgca caagtcctaa gttacgcgac aggctgccgc cctgcccttt tcctggcgtt
     7561 ttcttgtcgc gtgttttagt cgcataaagt agaatacttg cgactagaac cggagacatt
     7621 acgccatgaa caagagcgcc gccgctggcc tgctgggcta tgcccgcgtc agcaccgacg
     7681 accaggactt gaccaaccaa cgggccgaac tgcacgcggc cggctgcacc aagctgtttt
     7741 ccgagaagat caccggcacc aggcgcgacc gcccggagct ggccaggatg cttgaccacc
     7801 tacgccctgg cgacgttgtg acagtgacca ggctagaccg cctggcccgc agcacccgcg
     7861 acctactgga cattgccgag cgcatccagg aggccggcgc gggcctgcgt agcctggcag
     7921 agccgtgggc cgacaccacc acgccggccg gccgcatggt gttgaccgtg ttcgccggca
     7981 ttgccgagtt cgagcgttcc ctaatcatcg accgcacccg gagcgggcgc gaggccgcca
     8041 aggcccgagg cgtgaagttt ggcccccgcc ctaccctcac cccggcacag atcgcgcacg
     8101 cccgcgagct gatcgaccag gaaggccgca ccgtgaaaga ggcggctgca ctgcttggcg
     8161 tgcatcgctc gaccctgtac cgcgcacttg agcgcagcga ggaagtgacg cccaccgagg
     8221 ccaggcggcg cggtgccttc cgtgaggacg cattgaccga ggccgacgcc ctggcggccg
     8281 ccgagaatga acgccaagag gaacaagcat gaaaccgcac caggacggcc aggacgaacc
     8341 gtttttcatt accgaagaga tcgaggcgga gatgatcgcg gccgggtacg tgttcgagcc
     8401 gcccgcgcac ctctcaaccg tgcggctgca tgaaatcctg gccggtttgt ctgatgccaa
     8461 gctggcggcc tggccggcca gcttggccgc tgaagaaacc gagcgccgcc gtctaaaaag
     8521 gtgatgtgta tttgagtaaa acagcttgcg tcatgcggtc gctgcgtata tgatgcgatg
     8581 agtaaataaa caaatacgca aggggaacgc atgaaggtta tcgctgtact taaccagaaa
     8641 ggcgggtcag gcaagacgac catcgcaacc catctagccc gcgccctgca actcgccggg
     8701 gccgatgttc tgttagtcga ttccgatccc cagggcagtg cccgcgattg ggcggccgtg
     8761 cgggaagatc aaccgctaac cgttgtcggc atcgaccgcc cgacgattga ccgcgacgtg
     8821 aaggccatcg gccggcgcga cttcgtagtg atcgacggag cgccccaggc ggcggacttg
     8881 gctgtgtccg cgatcaaggc agccgacttc gtgctgattc cggtgcagcc aagcccttac
     8941 gacatatggg ccaccgccga cctggtggag ctggttaagc agcgcattga ggtcacggat
     9001 ggaaggctac aagcggcctt tgtcgtgtcg cgggcgatca aaggcacgcg catcggcggt
     9061 gaggttgccg aggcgctggc cgggtacgag ctgcccattc ttgagtcccg tatcacgcag
     9121 cgcgtgagct acccaggcac tgccgccgcc ggcacaaccg ttcttgaatc agaacccgag
     9181 ggcgacgctg cccgcgaggt ccaggcgctg gccgctgaaa ttaaatcaaa actcatttga
     9241 gttaatgagg taaagagaaa atgagcaaaa gcacaaacac gctaagtgcc ggccgtccga
     9301 gcgcacgcag cagcaaggct gcaacgttgg ccagcctggc agacacgcca gccatgaagc
     9361 gggtcaactt tcagttgccg gcggaggatc acaccaagct gaagatgtac gcggtacgcc
     9421 aaggcaagac cattaccgag ctgctatctg aatacatcgc gcagctacca gagtaaatga
     9481 gcaaatgaat aaatgagtag atgaatttta gcggctaaag gaggcggcat ggaaaatcaa
     9541 gaacaaccag gcaccgacgc cgtggaatgc cccatgtgtg gaggaacggg cggttggcca
     9601 ggcgtaagcg gctgggttgc ctgccggccc tgcaatggca ctggaacccc caagcccgag
     9661 gaatcggcgt gagcggtcgc aaaccatccg gcccggtaca aatcggcgcg gcgctgggtg
     9721 atgacctggt ggagaagttg aaggccgcgc aggccgccca gcggcaacgc atcgaggcag
     9781 aagcacgccc cggtgaatcg tggcaagcgg ccgctgatcg aatccgcaaa gaatcccggc
     9841 aaccgccggc agccggtgcg ccgtcgatta ggaagccgcc caagggcgac gagcaaccag
     9901 attttttcgt tccgatgctc tatgacgtgg gcacccgcga tagtcgcagc atcatggacg
     9961 tggccgtttt ccgtctgtcg aagcgtgacc gacgagctgg cgaggtgatc cgctacgagc
    10021 ttccagacgg gcacgtagag gtttccgcag ggccggccgg catggccagt gtgtgggatt
    10081 acgacctggt actgatggcg gtttcccatc taaccgaatc catgaaccga taccgggaag
    10141 ggaagggaga caagcccggc cgcgtgttcc gtccacacgt tgcggacgta ctcaagttct
    10201 gccggcgagc cgatggcgga aagcagaaag acgacctggt agaaacctgc attcggttaa
    10261 acaccacgca cgttgccatg cagcgtacga agaaggccaa gaacggccgc ctggtgacgg
    10321 tatccgaggg tgaagccttg attagccgct acaagatcgt aaagagcgaa accgggcggc
    10381 cggagtacat cgagatcgag ctagctgatt ggatgtaccg cgagatcaca gaaggcaaga
    10441 acccggacgt gctgacggtt caccccgatt actttttgat cgatcccggc atcggccgtt
    10501 ttctctaccg cctggcacgc cgcgccgcag gcaaggcaga agccagatgg ttgttcaaga
    10561 cgatctacga acgcagtggc agcgccggag agttcaagaa gttctgtttc accgtgcgca
    10621 agctgatcgg gtcaaatgac ctgccggagt acgatttgaa ggaggaggcg gggcaggctg
    10681 gcccgatcct agtcatgcgc taccgcaacc tgatcgaggg cgaagcatcc gccggttcct
    10741 aatgtacgga gcagatgcta gggcaaattg ccctagcagg ggaaaaaggt cgaaaaggac
    10801 tctttcctgt ggatagcacg tacattggga acccaaagcc gtacattggg aaccggaacc
    10861 cgtacattgg gaacccaaag ccgtacattg ggaaccggtc acacatgtaa gtgactgata
    10921 taaaagagaa aaaaggcgat ttttccgcct aaaactcttt aaaacttatt aaaactctta
    10981 aaacccgcct ggcctgtgca taactgtctg gccagcgcac agccgaagag ctgcaaaaag
    11041 cgcctaccct tcggtcgctg cgctccctac gccccgccgc ttcgcgtcgg cctatcgcgg
    11101 ccgctggccg ctcaaaaatg gctggcctac ggccaggcaa tctaccaggg cgcggacaag
    11161 ccgcgccgtc gccactcgac cgccggcgcc cacatcaagg caccctgcct cgcgcgtttc
    11221 ggtgatgacg gtgaaaacct ctgacacatg cagctcccgg tgacggtcac agcttgtctg
    11281 taagcggatg ccgggagcag acaagcccgt cagggcgcgt cagcgggtgt tggcgggtgt
    11341 cggggcgcag ccatgaccca gtcacgtagc gatagcggag tgtatactgg cttaactatg
    11401 cggcatcaga gcagattgta ctgagagtgc accatatgcg gtgtgaaata ccgcacagat
    11461 gcgtaaggag aaaataccgc atcaggcgct cttccgcttc ctcgctcact gactcgctgc
    11521 gctcggtcgt tcggctgcgg cgagcggtat cagctcactc aaaggcggta atacggttat
    11581 ccacagaatc aggggataac gcaggaaaga acatgtgagc aaaaggccag caaaaggcca
    11641 ggaaccgtaa aaaggccgcg ttgctggcgt ttttccatag gctccgcccc cctgacgagc
    11701 atcacaaaaa tcgacgctca agtcagaggt ggcgaaaccc gacaggacta taaagatacc
    11761 aggcgtttcc ccctggaagc tccctcgtgc gctctcctgt tccgaccctg ccgcttaccg
    11821 gatacctgtc cgcctttctc ccttcgggaa gcgtggcgct ttctcatagc tcacgctgta
    11881 ggtatctcag ttcggtgtag gtcgttcgct ccaagctggg ctgtgtgcac gaaccccccg
    11941 ttcagcccga ccgctgcgcc ttatccggta actatcgtct tgagtccaac ccggtaagac
    12001 acgacttatc gccactggca gcagccactg gtaacaggat tagcagagcg aggtatgtag
    12061 gcggtgctac agagttcttg aagtggtggc ctaactacgg ctacactaga aggacagtat
    12121 ttggtatctg cgctctgctg aagccagtta ccttcggaaa aagagttggt agctcttgat
    12181 ccggcaaaca aaccaccgct ggtagcggtg gtttttttgt ttgcaagcag cagattacgc
    12241 gcagaaaaaa aggatctcaa gaagatcctt tgatcttttc tacggggtct gacgctcagt
    12301 ggaacgaaaa ctcacgttaa gggattttgg tcatgcattc taggtgatta tttgccgact
    12361 accttggtga tctcgccttt cacgtagtgg acaaattctt ccaactgatc tgcgcgcgag
    12421 gccaagcgat cttcttcttg tccaagataa gcctgtctag cttcaagtat gacgggctga
    12481 tactgggccg gcaggcgctc cattgcccag tcggcagcga catccttcgg cgcgattttg
    12541 ccggttactg cgctgtacca aatgcgggac aacgtaagca ctacatttcg ctcatcacca
    12601 gcccagtcgg gcggcgagtt ccatagcgtt aaggtttcat ttagcgcctc aaatagatcc
    12661 tgttcaggaa ccggatcaaa gagttcctcc gccgctggac ctaccaaggc aacgctatgt
    12721 tctcttgctt ttgtcagcaa gatagccaga tcaatgtcga tcgtggctgg ctcgaagata
    12781 cctgcaagaa tgtcattgcg ctgccattct ccaaattgca gttcgcgctt agctggataa
    12841 cgccacggaa tgatgtcgtc gtgcacaaca atggtgactt ctacagcgcg gagaatctcg
    12901 ctctctccag gggaagccga agtttccaaa aggtcgttga tcaaagctcg ccgcgttgtt
    12961 tcatcaagcc ttacggtcac cgtaaccagc aaatcaatat cactgtgtgg cttcaggccg
    13021 ccatccactg cggagccgta caaatgtacg gccagcaacg tcggttcgag atggcgctcg
    13081 atgacgccaa ctacctctga tagttgagtc gatacttcgg cgatcaccgc ttccctcata
    13141 atgtttaact ttgttttagg gcgactgccc tgctgcgtaa catcgttgct gctccataac
    13201 atcaaacatc gacccacggc gtaacgcgct tgctgcttgg atgcccgagg catagactgt
    13261 accccaaaaa aacagtcata acaagccatg aaaaccgcca ctgcgccgtt accaccgctg
    13321 cgttcggtca aggttctgga ccagttgcgt gagcgcatac gctacttgca ttacagctta
    13381 cgaaccgaac aggcttatgt ccactgggtt cgtgccttca tccgtttcca cggtgtgcgt
    13441 cacccggcaa ccttgggtag cagcgaagtc gaggcatttc tgtcctggct ggaacagaac
    13501 ttattatttc cttcctcttt tctacagtat ttaaagatac cccaagaagc taattataac
    13561 aagacgaact ccaattcact gttccttgca ttctaaaacc ttaaatacca gaaaacagct
    13621 ttttcaaagt tgttttcaaa gttggcgtat aacatagtat cgacggagcc gattttgaaa
    13681 ccgcggtgat cacaggcagc aacgctctgt catcgttaca atcaacatgc taccctccgc
    13741 gagatcatcc gtgtttcaaa cccggcagct tagttgccgt tcttccgaat agcatcggta
    13801 acatgagcaa agtctgccgc cttacaacgg ctctcccgct gacgccgtcc cggactgatg
    13861 ggctgcctgt atcgagtggt gattttgtgc cgagctgccg gtcggggagc tgttggctgg
    13921 ctggtggcag gatatattgt ggtgtaaaca taacggatcc ggtctcag
//
