LOCUS       sequence-410740-        9590 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Mammalian expression of the human LTB4 receptor LTB4R with the T308A
            and S310A mutations with an N-terminal 3x HA tag..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9590)
  AUTHORS   Lundgren SM, Rocha-Gregg BL, Akdogan E, Mysore MN, Hayes S, Collins 
            SR
  TITLE     Signaling dynamics distinguish high- and low-priority neutrophil 
            chemoattractant receptors.
  JOURNAL   Sci Signal. 2023 Oct 3;16(805):eadd1845. doi: 
            10.1126/scisignal.add1845. Epub 2023 Oct 3.
  PUBMED    37788324
REFERENCE   2  (bases 1 to 9590)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Sci 
            Signal."; date: "2023-10-3"; volume: "16(805):eadd1845. doi"; pages:
            " 10.1126/scisignal.add1845. Epub 2023 Oct 3"
COMMENT     Sequence Label: pLV_PGK_3xHA-LTB4R(T308A,S310A)
FEATURES             Location/Qualifiers
     source          1..9590
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    1..234
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             419..463
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    726..841
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1 (lacking the first T)"
     promoter        887..1397
                     /label=hPGK promoter
                     /note="human phosphoglycerate kinase 1 promoter"
     primer_bind     1316..1334
                     /label=hPGK-F
                     /note="Human PGK promoter, forward primer"
     CDS             1419..1499
                     /codon_start=1
                     /product="three tandem HA epitope tags"
                     /label=3xHA
                     /translation="YPYDVPDYAYPYDVPDYAYPYDVPDYA"
     misc_feature    2656..3205
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(2800..2817)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     3027..3046
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     CDS             3208..4233
                     /codon_start=1
                     /product="hygromycin B phosphotransferase"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
                     E"
     CDS             3208..4233
                     /codon_start=1
                     /gene="aph(4)-Ia"
                     /product="aminoglycoside phosphotransferase from E. coli"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
                     E"
     misc_feature    4257..4845
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(4310..4330)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(4728..4739)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             5052..5685
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     primer_bind     complement(5813..5829)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(5813..5829)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(5826..5848)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    5837..5853
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(5861..5867,5868..5885,5886..5891))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    5906..5927
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(6044..6061)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(6215..6803)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(6295..6314)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(6974..7765,7766..7834))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     7597..7616
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(7835..7939)
                     /gene="bla"
                     /label=AmpR promoter
     polyA_signal    7987..8121
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(8024..8043)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     8078..8097
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     LTR             8289..8922
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     misc_feature    8969..9094
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
ORIGIN
        1 aggagctttg ttccttgggt tcttgggagc agcaggaagc actatgggcg cagcgtcaat
       61 gacgctgacg gtacaggcca gacaattatt gtctggtata gtgcagcagc agaacaattt
      121 gctgagggct attgaggcgc aacagcatct gttgcaactc acagtctggg gcatcaagca
      181 gctccaggca agaatcctgg ctgtggaaag atacctaaag gatcaacagc tcctggggat
      241 ttggggttgc tctggaaaac tcatttgcac cactgctgtg ccttggaatg ctagttggag
      301 taataaatct ctggaacaga tttggaatca cacgacctgg atggagtggg acagagaaat
      361 taacaattac acaagcttaa tacactcctt aattgaagaa tcgcaaaacc agcaagaaaa
      421 gaatgaacaa gaattattgg aattagataa atgggcaagt ttgtggaatt ggtttaacat
      481 aacaaattgg ctgtggtata taaaattatt cataatgata gtaggaggct tggtaggttt
      541 aagaatagtt tttgctgtac tttctatagt gaatagagtt aggcagggat attcaccatt
      601 atcgtttcag acccacctcc caaccccgag gggacccgac aggcccgaag gaatagaaga
      661 agaaggtgga gagagagaca gagacagatc cattcgatta gtgaacggat ctcgacggta
      721 tcgcctttaa aagaaaaggg gggattgggg ggtacagtgc aggggaaaga atagtagaca
      781 taatagcaac agacatacaa actaaagaat tacaaaaaca aattacaaaa attcaaaatt
      841 ttcgggttta ttacagggac agcagagatc cagtttatcg atccacgggg ttggggttgc
      901 gccttttcca aggcagccct gggtttgcgc agggacgcgg ctgctctggg cgtggttccg
      961 ggaaacgcag cggcgccgac cctgggtctc gcacattctt cacgtccgtt cgcagcgtca
     1021 cccggatctt cgccgctacc cttgtgggcc ccccggcgac gcttcctgct ccgcccctaa
     1081 gtcgggaagg ttccttgcgg ttcgcggcgt gccggacgtg acaaacggaa gccgcacgtc
     1141 tcactagtac cctcgcagac ggacagcgcc agggagcaat ggcagcgcgc cgaccgcgat
     1201 gggctgtggc caatagcggc tgctcagcag ggcgcgccga gagcagcggc cgggaagggg
     1261 cggtgcggga ggcggggtgt ggggcggtag tgtgggccct gttcctgccc gcgcggtgtt
     1321 ccgcattctg caagcctccg gagcgcacgt cggcagtcgg ctccctcgtt gaccgaatca
     1381 ccgacctctc tccccaggac gcgggatccg ccaccatgta cccatacgat gttccagatt
     1441 acgcttaccc atacgatgtt ccagattacg cttacccata cgatgttcca gattacgcta
     1501 acactacatc ttctgcagca cccccctcac taggtgtaga gttcatctct ctgctggcta
     1561 tcatcctgct gtcagtggcg ctggctgtgg ggcttcccgg caacagcttt gtggtgtgga
     1621 gtatcctgaa aaggatgcag aagcgctctg tcactgccct gatggtgctg aacctggccc
     1681 tggccgacct ggccgtattg ctcactgctc cctttttcct tcacttcctg gcccaaggca
     1741 cctggagttt tggactggct ggttgccgcc tgtgtcacta tgtctgcgga gtcagcatgt
     1801 acgccagcgt cctgcttatc acggccatga gtctagaccg ctcactggcg gtggcccgcc
     1861 cctttgtgtc ccagaagcta cgcaccaagg cgatggcccg gcgggtgctg gcaggcatct
     1921 gggtgttgtc ctttctgctg gccacacccg tcctcgcgta ccgcacagta gtgccctgga
     1981 aaacgaacat gagcctgtgc ttcccgcggt accccagcga agggcaccgg gccttccatc
     2041 taatcttcga ggctgtcacg ggcttcctgc tgcccttcct ggctgtggtg gccagctact
     2101 cggacatagg gcgtcggcta caggcccggc gcttccgccg cagccgccgc accggccgcc
     2161 tggtggtgct catcatcctg accttcgccg ccttctggct gccctaccac gtggtgaacc
     2221 tggctgaggc gggccgcgcg ctggccggcc aggccgccgg gttagggctc gtggggaagc
     2281 ggctgagcct ggcccgcaac gtgctcatcg cactcgcctt cctgagcagc agcgtgaacc
     2341 ccgtgctgta cgcgtgcgcc ggcggcggcc tgctgcgctc ggcgggcgtg ggcttcgtcg
     2401 ccaagctgct ggagggcgcg ggcgccgagg cgtccagcac gcgccgcggg ggcagcctgg
     2461 gccagaccgc taggagcggc cccgccgctc tggagcccgg cccttccgag agcctcactg
     2521 cctccagccc tctcaagtta aacgaactga actaagcggc cgctctagag tcgacgggcc
     2581 gcggtaacaa ttgttaacta acttaagcta gcaacggttt ccctctagcg ggatcaattc
     2641 cgcccccccc ccctaacgtt actggccgaa gccgcttgga ataaggccgg tgtgcgtttg
     2701 tctatatgtt attttccacc atattgccgt cttttggcaa tgtgagggcc cggaaacctg
     2761 gccctgtctt cttgacgagc attcctaggg gtctttcccc tctcgccaaa ggaatgcaag
     2821 gtctgttgaa tgtcgtgaag gaagcagttc ctctggaagc ttcttgaaga caaacaacgt
     2881 ctgtagcgac cctttgcagg cagcggaacc ccccacctgg cgacaggtgc ctctgcggcc
     2941 aaaagccacg tgtataagat acacctgcaa aggcggcaca accccagtgc cacgttgtga
     3001 gttggatagt tgtggaaaga gtcaaatggc tctcctcaag cgtattcaac aaggggctga
     3061 aggatgccca gaaggtaccc cattgtatgg gatctgatct ggggcctcgg tgcacatgct
     3121 ttacatgtgt ttagtcgagg ttaaaaaaac gtctaggccc cccgaaccac ggggacgtgg
     3181 ttttcctttg aaaaacacga taataccatg aaaaagcctg aactcaccgc gacgtctgtc
     3241 gagaagtttc tgatcgaaaa gttcgacagc gtctccgacc tgatgcagct ctcggagggc
     3301 gaagaatctc gtgctttcag cttcgatgta ggagggcgtg gatatgtcct gcgggtaaat
     3361 agctgcgccg atggtttcta caaagatcgt tatgtttatc ggcactttgc atcggccgcg
     3421 ctcccgattc cggaagtgct tgacattggg gaattcagcg agagcctgac ctattgcatc
     3481 tcccgccgtg cacagggtgt cacgttgcaa gacctgcctg aaaccgaact gcccgctgtt
     3541 ctgcagccgg tcgcggaggc catggatgcg atcgctgcgg ccgatcttag ccagacgagc
     3601 gggttcggcc cattcggacc gcaaggaatc ggtcaataca ctacatggcg tgatttcata
     3661 tgcgcgattg ctgatcccca tgtgtatcac tggcaaactg tgatggacga caccgtcagt
     3721 gcgtccgtcg cgcaggctct cgatgagctg atgctttggg ccgaggactg ccccgaagtc
     3781 cggcacctcg tgcacgcgga tttcggctcc aacaatgtcc tgacggacaa tggccgcata
     3841 acagcggtca ttgactggag cgaggcgatg ttcggggatt cccaatacga ggtcgccaac
     3901 atcttcttct ggaggccgtg gttggcttgt atggagcagc agacgcgcta cttcgagcgg
     3961 aggcatccgg agcttgcagg atcgccgcgg ctccgggcgt atatgctccg cattggtctt
     4021 gaccaactct atcagagctt ggttgacggc aatttcgatg atgcagcttg ggcgcagggt
     4081 cgatgcgacg caatcgtccg atccggagcc gggactgtcg ggcgtacaca aatcgcccgc
     4141 agaagcgcgg ccgtctggac cgatggctgt gtagaagtac tcgccgatag tggaaaccga
     4201 cgccccagca ctcgtccgag ggcaaaggaa tagcccgggc ggggcgcgtc tggaacaatc
     4261 aacctctgga ttacaaaatt tgtgaaagat tgactggtat tcttaactat gttgctcctt
     4321 ttacgctatg tggatacgct gctttaatgc ctttgtatca tgctattgct tcccgtatgg
     4381 ctttcatttt ctcctccttg tataaatcct ggttgctgtc tctttatgag gagttgtggc
     4441 ccgttgtcag gcaacgtggc gtggtgtgca ctgtgtttgc tgacgcaacc cccactggtt
     4501 ggggcattgc caccacctgt cagctccttt ccgggacttt cgctttcccc ctccctattg
     4561 ccacggcgga actcatcgcc gcctgccttg cccgctgctg gacaggggct cggctgttgg
     4621 gcactgacaa ttccgtggtg ttgtcgggga agctgacgtc ctttccatgg ctgctcgcct
     4681 gtgttgccac ctggattctg cgcgggacgt ccttctgcta cgtcccttcg gccctcaatc
     4741 cagcggacct tccttcccgc ggcctgctgc cggctctgcg gcctcttccg cgtcttcgcc
     4801 ttcgccctca gacgagtcgg atctcccttt gggccgcctc cccgcctgga attaattctg
     4861 cagtcgagac ctagaaaaac atggagcaat cacaagtagc aatacagcag ctaccaatgc
     4921 tgattgtgcc tggctagaag cacaagagga ggaggaggtg ggttttccag tcacacctca
     4981 ggtaccttta agaccaatga cttacaaggc agctgtagat cttagccact ttttaaaaga
     5041 aaagagggga ctggaagggc taattcactc ccaacgaaga caagatatcc ttgatctgtg
     5101 gatctaccac acacaaggct acttccctga ttagcagaac tacacaccag ggccaggggt
     5161 cagatatcca ctgacctttg gatggtgcta caagctagta ccagttgagc cagataaggt
     5221 agaagaggcc aataaaggag agaacaccag cttgttacac cctgtgagcc tgcatgggat
     5281 ggatgacccg gagagagaag tgttagagtg gaggtttgac agccgcctag catttcatca
     5341 cgtggcccga gagctgcatc cggagtactt caagaactgc tgatatcgag cttgctacaa
     5401 gggactttcc gctggggact ttccagggag gcgtggcctg ggcgggactg gggagtggcg
     5461 agccctcaga tcctgcatat aagcagctgc tttttgcctg tactgggtct ctctggttag
     5521 accagatctg agcctgggag ctctctggct aactagggaa cccactgctt aagcctcaat
     5581 aaagcttgcc ttgagtgctt caagtagtgt gtgcccgtct gttgtgtgac tctggtaact
     5641 agagatccct cagacccttt tagtcagtgt ggaaaatctc tagcagtagt agttcatgtc
     5701 atcttattat tcagtattta taacttgcaa agaaatgaat atcagagagt gagaggcctt
     5761 gacattgcta gcgtttaccg tcgacctcta gctagagctt ggcgtaatca tggtcatagc
     5821 tgtttcctgt gtgaaattgt tatccgctca caattccaca caacatacga gccggaagca
     5881 taaagtgtaa agcctggggt gcctaatgag tgagctaact cacattaatt gcgttgcgct
     5941 cactgcccgc tttccagtcg ggaaacctgt cgtgccagct gcattaatga atcggccaac
     6001 gcgcggggag aggcggtttg cgtattgggc gctcttccgc ttcctcgctc actgactcgc
     6061 tgcgctcggt cgttcggctg cggcgagcgg tatcagctca ctcaaaggcg gtaatacggt
     6121 tatccacaga atcaggggat aacgcaggaa agaacatgtg agcaaaaggc cagcaaaagg
     6181 ccaggaaccg taaaaaggcc gcgttgctgg cgtttttcca taggctccgc ccccctgacg
     6241 agcatcacaa aaatcgacgc tcaagtcaga ggtggcgaaa cccgacagga ctataaagat
     6301 accaggcgtt tccccctgga agctccctcg tgcgctctcc tgttccgacc ctgccgctta
     6361 ccggatacct gtccgccttt ctcccttcgg gaagcgtggc gctttctcat agctcacgct
     6421 gtaggtatct cagttcggtg taggtcgttc gctccaagct gggctgtgtg cacgaacccc
     6481 ccgttcagcc cgaccgctgc gccttatccg gtaactatcg tcttgagtcc aacccggtaa
     6541 gacacgactt atcgccactg gcagcagcca ctggtaacag gattagcaga gcgaggtatg
     6601 taggcggtgc tacagagttc ttgaagtggt ggcctaacta cggctacact agaagaacag
     6661 tatttggtat ctgcgctctg ctgaagccag ttaccttcgg aaaaagagtt ggtagctctt
     6721 gatccggcaa acaaaccacc gctggtagcg gtggtttttt tgtttgcaag cagcagatta
     6781 cgcgcagaaa aaaaggatct caagaagatc ctttgatctt ttctacgggg tctgacgctc
     6841 agtggaacga aaactcacgt taagggattt tggtcatgag attatcaaaa aggatcttca
     6901 cctagatcct tttaaattaa aaatgaagtt ttaaatcaat ctaaagtata tatgagtaaa
     6961 cttggtctga cagttaccaa tgcttaatca gtgaggcacc tatctcagcg atctgtctat
     7021 ttcgttcatc catagttgcc tgactccccg tcgtgtagat aactacgata cgggagggct
     7081 taccatctgg ccccagtgct gcaatgatac cgcgagaccc acgctcaccg gctccagatt
     7141 tatcagcaat aaaccagcca gccggaaggg ccgagcgcag aagtggtcct gcaactttat
     7201 ccgcctccat ccagtctatt aattgttgcc gggaagctag agtaagtagt tcgccagtta
     7261 atagtttgcg caacgttgtt gccattgcta caggcatcgt ggtgtcacgc tcgtcgtttg
     7321 gtatggcttc attcagctcc ggttcccaac gatcaaggcg agttacatga tcccccatgt
     7381 tgtgcaaaaa agcggttagc tccttcggtc ctccgatcgt tgtcagaagt aagttggccg
     7441 cagtgttatc actcatggtt atggcagcac tgcataattc tcttactgtc atgccatccg
     7501 taagatgctt ttctgtgact ggtgagtact caaccaagtc attctgagaa tagtgtatgc
     7561 ggcgaccgag ttgctcttgc ccggcgtcaa tacgggataa taccgcgcca catagcagaa
     7621 ctttaaaagt gctcatcatt ggaaaacgtt cttcggggcg aaaactctca aggatcttac
     7681 cgctgttgag atccagttcg atgtaaccca ctcgtgcacc caactgatct tcagcatctt
     7741 ttactttcac cagcgtttct gggtgagcaa aaacaggaag gcaaaatgcc gcaaaaaagg
     7801 gaataagggc gacacggaaa tgttgaatac tcatactctt cctttttcaa tattattgaa
     7861 gcatttatca gggttattgt ctcatgagcg gatacatatt tgaatgtatt tagaaaaata
     7921 aacaaatagg ggttccgcgc acatttcccc gaaaagtgcc acctgacgtc gacggatcgg
     7981 gagatcaact tgtttattgc agcttataat ggttacaaat aaagcaatag catcacaaat
     8041 ttcacaaata aagcattttt ttcactgcat tctagttgtg gtttgtccaa actcatcaat
     8101 gtatcttatc atgtctggat caactggata actcaagcta accaaaatca tcccaaactt
     8161 cccaccccat accctattac cactgccaat tacctgtggt ttcatttact ctaaacctgt
     8221 gattcctctg aattattttc attttaaaga aattgtattt gttaaatatg tactacaaac
     8281 ttagtagttg gaagggctaa ttcactccca aagaagacaa gatatccttg atctgtggat
     8341 ctaccacaca caaggctact tccctgatta gcagaactac acaccagggc caggggtcag
     8401 atatccactg acctttggat ggtgctacaa gctagtacca gttgagccag ataaggtaga
     8461 agaggccaat aaaggagaga acaccagctt gttacaccct gtgagcctgc atgggatgga
     8521 tgacccggag agagaagtgt tagagtggag gtttgacagc cgcctagcat ttcatcacgt
     8581 ggcccgagag ctgcatccgg agtacttcaa gaactgctga tatcgagctt gctacaaggg
     8641 actttccgct ggggactttc cagggaggcg tggcctgggc gggactgggg agtggcgagc
     8701 cctcagatcc tgcatataag cagctgcttt ttgcctgtac tgggtctctc tggttagacc
     8761 agatctgagc ctgggagctc tctggctaac tagggaaccc actgcttaag cctcaataaa
     8821 gcttgccttg agtgcttcaa gtagtgtgtg cccgtctgtt gtgtgactct ggtaactaga
     8881 gatccctcag acccttttag tcagtgtgga aaatctctag cagtggcgcc cgaacaggga
     8941 cttgaaagcg aaagggaaac cagaggagct ctctcgacgc aggactcggc ttgctgaagc
     9001 gcgcacggca agaggcgagg ggcggcgact ggtgagtacg ccaaaaattt tgactagcgg
     9061 aggctagaag gagagagatg ggtgcgagag cgtcagtatt aagcggggga gaattagatc
     9121 gcgatgggaa aaaattcggt taaggccagg gggaaagaaa aaatataaat taaaacatat
     9181 agtatgggca agcagggagc tagaacgatt cgcagttaat cctggcctgt tagaaacatc
     9241 agaaggctgt agacaaatac tgggacagct acaaccatcc cttcagacag gatcagaaga
     9301 acttagatca ttatataata cagtagcaac cctctattgt gtgcatcaaa ggatagagat
     9361 aaaagacacc aaggaagctt tagacaagat agaggaagag caaaacaaaa gtaagaccac
     9421 cgcacagcaa gcggccggcc gctgatcttc agacctggag gaggagatat gagggacaat
     9481 tggagaagtg aattatataa atataaagta gtaaaaattg aaccattagg agtagcaccc
     9541 accaaggcaa agagaagagt ggtgcagaga gaaaaaagag cagtgggaat
//
