LOCUS       sequence-410744-       15286 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Glucos(1-231)-NpuDnaE(N).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 15286)
  AUTHORS   Yuan G, Lu H, De K, Hassan MM, Liu Y, Islam MT, Muchero W, Tuskan 
            GA, Yang X
  TITLE     Split selectable marker systems utilizing inteins facilitate gene 
            stacking in plants.
  JOURNAL   Commun Biol. 2023 May 26;6(1):567. doi: 10.1038/s42003-023-04950-8.
  PUBMED    37237044
REFERENCE   2  (bases 1 to 15286)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Commun 
            Biol."; date: "2023-05-26"; volume: "6(1):567. doi"; pages: " 
            10.1038/s42003-023-04950-8"
COMMENT     Sequence Label: pAXY0006
FEATURES             Location/Qualifiers
     source          1..15286
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             331..348
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     terminator      380..632
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     misc_feature    654..678
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     CDS             1979..2608
                     /codon_start=1
                     /product="stability protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 StaA
                     /translation="MKVIAVLNQKGGSGKTTIATHLARALQLAGADVLLVDSDPQGSAR
                     DWAAVREDQPLTVVGIDRPTIDRDVKAIGRRDFVVIDGAPQAADLAVSAIKAADFVLIP
                     VQPSPYDIWATADLVELVKQRIEVTDGRLQAAFVVSRAIKGTRIGGEVAEALAGYELPI
                     LESRITQRVSYPGTAAAGTTVLESEPEGDAAREVQALAAEIKSKLI"
     CDS             3037..4110
                     /codon_start=1
                     /product="replication protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 RepA
                     /translation="MSGRKPSGPVQIGAALGDDLVEKLKAAQAAQRQRIEAEARPGESW
                     QAAADRIRKESWQPPAAGAPSIRKPPKGDEQPDFFVPMLYDVGTRDSRSIMDVAVFRLS
                     KRDRRAGEVIRYELPDGHVEVSAGPAGMASVWDYDLVLMAVSHLTESMNRYREGKGDKP
                     GRVFRPHVADVLKFCRRADGGKQKDDLVETCIRLNTTHVAMQRTKKAKNGRLVTVSEGE
                     ALISRYKIVKSETGRPEYIEIELADWMYREITEGKNPDVLTVHPDYFLIDPGIGRFLYR
                     LARRAAGKAEARWLFKTIYERSGSAGEFKKFCFTVRKLIGSNDLPEYDLKEEAGQAGPI
                     LVMRYRNLIEGEASAGS"
     rep_origin      4176..4370
                     /label=pVS1 oriV
                     /note="origin of replication for the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
     primer_bind     complement(4606..4628)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     misc_feature    4714..4854
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     4769..4788
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     complement(4869..4886)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(5040..5628)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(5120..5139)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(5715..6509)
                     /codon_start=1
                     /gene="aphA-3"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin"
                     /translation="MAKMRISPELKKLIEKYRCVKDTEGMSPAKVYKLVGENENLYLKM
                     TDSRYKGTTYDVEREKDMMLWLEGKLPVPKVLHFERHDGWSNLLMSEADGVLCSEEYED
                     EQSPEKIIELYAECIRLFHSIDISDCPYTNSLDSRLAELDYLLNNDLADVDCENWEEDT
                     PFKDPRELYDFLKTEKPEEELVFSHGDLGDSNIFVKDGKVSGFIDLGRSGRADKWYDIA
                     FCVRSIREDIGEEQYVELFFDLLGIKPDWEKIKYYILLDELF"
     misc_feature    6934..6958
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     polyA_signal    7036..7210
                     /label=CaMV poly(A) signal
                     /note="cauliflower mosaic virus polyadenylation signal"
     CDS             complement(7250..8047)
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MGIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGR
                     PVLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLL
                     SSHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEH
                     QGLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDI
                     ALATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(7361..7380)
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     primer_bind     7971..7990
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     promoter        complement(8115..8791)
                     /label=CaMV 35S promoter (enhanced)
                     /note="cauliflower mosaic virus 35S promoter with a 
                     duplicated enhancer region"
     protein_bind    8982..9003
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(9018..9023,9024..9041,9042..9048)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    9056..9072
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     9061..9083
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     9080..9096
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     9080..9096
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     CDS             complement(10217..10273)
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
     promoter        complement(11788..12133)
                     /label=CaMV 35S promoter
                     /note="strong constitutive promoter from cauliflower mosaic
                     virus"
     primer_bind     complement(12648..12665)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(12648..12664)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(12657..12679)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     promoter        13041..13386
                     /label=CaMV 35S promoter
                     /note="strong constitutive promoter from cauliflower mosaic
                     virus"
     terminator      14405..14653
                     /label=HSP terminator
                     /note="efficient transcription terminator from the 
                     Arabidopsis thaliana heat shock protein 18.2 gene (Nagaya 
                     et al., 2010)"
ORIGIN
        1 tggaacaagc gttgcccagg aaagccgatc atgatcactg agtacggcgc agacaccgtt
       61 gcgggctttc acgacattga tccagtgatg ttcaccgagg aatatcaagt cgagtactac
      121 caggcgaacc acgtcgtgtt cgatgagttt gagaacttcg tgggtgagca agcgtggaac
      181 ttcgcggact tcgcgacctc tcagggcgtg atgcgcgtcc aaggaaacaa gaagggcgtg
      241 ttcactcgtg accgcaagcc gaagctcgcc gcgcacgtct ttcgcgagcg ctggaccaac
      301 attccagatt tcggctacaa gaacgctagc catcaccatc accatcacgt gtgaattggt
      361 gaccagctcg aatttccccg atcgttcaaa catttggcaa taaagtttct taagattgaa
      421 tcctgttgcc ggtcttgcga tgattatcat ataatttctg ttgaattacg ttaagcatgt
      481 aataattaac atgtaatgca tgacgttatt tatgagatgg gtttttatga ttagagtccc
      541 gcaattatac atttaatacg cgatagaaaa caaaatatag cgcgcaaact aggataaatt
      601 atcgcgcgcg gtgtcatcta tgttactaga tcgggaatta aactatcagt gtttgacagg
      661 atatattggc gggtaaacct aagagaaaag agcgtttatt agaataatcg gatatttaaa
      721 agggcgtgaa aaggtttatc cgttcgtcca tttgtatgtg catgccaacc acagggttcc
      781 cctcgggatc aaagtacttt gatccaaccc ctccgctgct atagtgcagt cggcttctga
      841 cgttcagtgc agccgtcttc tgaaaacgac atgtcgcaca agtcctaagt tacgcgacag
      901 gctgccgccc tgcccttttc ctggcgtttt cttgtcgcgt gttttagtcg cataaagtag
      961 aatacttgcg actagaaccg gagacattac gccatgaaca agagcgccgc cgctggcctg
     1021 ctgggctatg cccgcgtcag caccgacgac caggacttga ccaaccaacg ggccgaactg
     1081 cacgcggccg gctgcaccaa gctgttttcc gagaagatca ccggcaccag gcgcgaccgc
     1141 ccggagctgg ccaggatgct tgaccaccta cgccctggcg acgttgtgac agtgaccagg
     1201 ctagaccgcc tggcccgcag cacccgcgac ctactggaca ttgccgagcg catccaggag
     1261 gccggcgcgg gcctgcgtag cctggcagag ccgtgggccg acaccaccac gccggccggc
     1321 cgcatggtgt tgaccgtgtt cgccggcatt gccgagttcg agcgttccct aatcatcgac
     1381 cgcacccgga gcgggcgcga ggccgccaag gcccgaggcg tgaagtttgg cccccgccct
     1441 accctcaccc cggcacagat cgcgcacgcc cgcgagctga tcgaccagga aggccgcacc
     1501 gtgaaagagg cggctgcact gcttggcgtg catcgctcga ccctgtaccg cgcacttgag
     1561 cgcagcgagg aagtgacgcc caccgaggcc aggcggcgcg gtgccttccg tgaggacgca
     1621 ttgaccgagg ccgacgccct ggcggccgcc gagaatgaac gccaagagga acaagcatga
     1681 aaccgcacca ggacggccag gacgaaccgt ttttcattac cgaagagatc gaggcggaga
     1741 tgatcgcggc cgggtacgtg ttcgagccgc ccgcgcacgt ctcaaccgtg cggctgcatg
     1801 aaatcctggc cggtttgtct gatgccaagc tggcggcctg gccggccagc ttggccgctg
     1861 aagaaaccga gcgccgccgt ctaaaaaggt gatgtgtatt tgagtaaaac agcttgcgtc
     1921 atgcggtcgc tgcgtatatg atgcgatgag taaataaaca aatacgcaag gggaacgcat
     1981 gaaggttatc gctgtactta accagaaagg cgggtcaggc aagacgacca tcgcaaccca
     2041 tctagcccgc gccctgcaac tcgccggggc cgatgttctg ttagtcgatt ccgatcccca
     2101 gggcagtgcc cgcgattggg cggccgtgcg ggaagatcaa ccgctaaccg ttgtcggcat
     2161 cgaccgcccg acgattgacc gcgacgtgaa ggccatcggc cggcgcgact tcgtagtgat
     2221 cgacggagcg ccccaggcgg cggacttggc tgtgtccgcg atcaaggcag ccgacttcgt
     2281 gctgattccg gtgcagccaa gcccttacga catatgggcc accgccgacc tggtggagct
     2341 ggttaagcag cgcattgagg tcacggatgg aaggctacaa gcggcctttg tcgtgtcgcg
     2401 ggcgatcaaa ggcacgcgca tcggcggtga ggttgccgag gcgctggccg ggtacgagct
     2461 gcccattctt gagtcccgta tcacgcagcg cgtgagctac ccaggcactg ccgccgccgg
     2521 cacaaccgtt cttgaatcag aacccgaggg cgacgctgcc cgcgaggtcc aggcgctggc
     2581 cgctgaaatt aaatcaaaac tcatttgagt taatgaggta aagagaaaat gagcaaaagc
     2641 acaaacacgc taagtgccgg ccgtccgagc gcacgcagca gcaaggctgc aacgttggcc
     2701 agcctggcag acacgccagc catgaagcgg gtcaactttc agttgccggc ggaggatcac
     2761 accaagctga agatgtacgc ggtacgccaa ggcaagacca ttaccgagct gctatctgaa
     2821 tacatcgcgc agctaccaga gtaaatgagc aaatgaataa atgagtagat gaattttagc
     2881 ggctaaagga ggcggcatgg aaaatcaaga acaaccaggc accgacgccg tggaatgccc
     2941 catgtgtgga ggaacgggcg gttggccagg cgtaagcggc tgggttgcct gccggccctg
     3001 caatggcact ggaaccccca agcccgagga atcggcgtga gcggtcgcaa accatccggc
     3061 ccggtacaaa tcggcgcggc gctgggtgat gacctggtgg agaagttgaa ggccgcgcag
     3121 gccgcccagc ggcaacgcat cgaggcagaa gcacgccccg gtgaatcgtg gcaagcggcc
     3181 gctgatcgaa tccgcaaaga atcctggcaa ccgccggcag ccggtgcgcc gtcgattagg
     3241 aagccgccca agggcgacga gcaaccagat tttttcgttc cgatgctcta tgacgtgggc
     3301 acccgcgata gtcgcagcat catggacgtg gccgttttcc gtctgtcgaa gcgtgaccga
     3361 cgagctggcg aggtgatccg ctacgagctt ccagacgggc acgtagaggt ttccgcaggg
     3421 ccggccggca tggccagtgt gtgggattac gacctggtac tgatggcggt ttcccatcta
     3481 accgaatcca tgaaccgata ccgggaaggg aagggagaca agcccggccg cgtgttccgt
     3541 ccacacgttg cggacgtact caagttctgc cggcgagccg atggcggaaa gcagaaagac
     3601 gacctggtag aaacctgcat tcggttaaac accacgcacg ttgccatgca gcgtacgaag
     3661 aaggccaaga acggccgcct ggtgacggta tccgagggtg aagccttgat tagccgctac
     3721 aagatcgtaa agagcgaaac cgggcggccg gagtacatcg agatcgagct agctgattgg
     3781 atgtaccgcg agatcacaga aggcaagaac ccggacgtgc tgacggttca ccccgattac
     3841 tttttgatcg atcccggcat cggccgtttt ctctaccgcc tggcacgccg cgccgcaggc
     3901 aaggcagaag ccagatggtt gttcaagacg atctacgaac gcagtggcag cgccggagag
     3961 ttcaagaagt tctgtttcac cgtgcgcaag ctgatcgggt caaatgacct gccggagtac
     4021 gatttgaagg aggaggcggg gcaggctggc ccgatcctag tcatgcgcta ccgcaacctg
     4081 atcgagggcg aagcatccgc cggttcctaa tgtacggagc agatgctagg gcaaattgcc
     4141 ctagcagggg aaaaaggtcg aaaaggtctc tttcctgtgg atagcacgta cattgggaac
     4201 ccaaagccgt acattgggaa ccggaacccg tacattggga acccaaagcc gtacattggg
     4261 aaccggtcac acatgtaagt gactgatata aaagagaaaa aaggcgattt ttccgcctaa
     4321 aactctttaa aacttattaa aactcttaaa acccgcctgg cctgtgcata actgtctggc
     4381 cagcgcacag ccgaagagct gcaaaaagcg cctacccttc ggtcgctgcg ctccctacgc
     4441 cccgccgctt cgcgtcggcc tatcgcggcc gctggccgct caaaaatggc tggcctacgg
     4501 ccaggcaatc taccagggcg cggacaagcc gcgccgtcgc cactcgaccg ccggcgccca
     4561 catcaaggca ccctgcctcg cgcgtttcgg tgatgacggt gaaaacctct gacacatgca
     4621 gctcccggag acggtcacag cttgtctgta agcggatgcc gggagcagac aagcccgtca
     4681 gggcgcgtca gcgggtgttg gcgggtgtcg gggcgcagcc atgacccagt cacgtagcga
     4741 tagcggagtg tatactggct taactatgcg gcatcagagc agattgtact gagagtgcac
     4801 catatgcggt gtgaaatacc gcacagatgc gtaaggagaa aataccgcat caggcgctct
     4861 tccgcttcct cgctcactga ctcgctgcgc tcggtcgttc ggctgcggcg agcggtatca
     4921 gctcactcaa aggcggtaat acggttatcc acagaatcag gggataacgc aggaaagaac
     4981 atgtgagcaa aaggccagca aaaggccagg aaccgtaaaa aggccgcgtt gctggcgttt
     5041 ttccataggc tccgcccccc tgacgagcat cacaaaaatc gacgctcaag tcagaggtgg
     5101 cgaaacccga caggactata aagataccag gcgtttcccc ctggaagctc cctcgtgcgc
     5161 tctcctgttc cgaccctgcc gcttaccgga tacctgtccg cctttctccc ttcgggaagc
     5221 gtggcgcttt ctcatagctc acgctgtagg tatctcagtt cggtgtaggt cgttcgctcc
     5281 aagctgggct gtgtgcacga accccccgtt cagcccgacc gctgcgcctt atccggtaac
     5341 tatcgtcttg agtccaaccc ggtaagacac gacttatcgc cactggcagc agccactggt
     5401 aacaggatta gcagagcgag gtatgtaggc ggtgctacag agttcttgaa gtggtggcct
     5461 aactacggct acactagaag gacagtattt ggtatctgcg ctctgctgaa gccagttacc
     5521 ttcggaaaaa gagttggtag ctcttgatcc ggcaaacaaa ccaccgctgg tagcggtggt
     5581 ttttttgttt gcaagcagca gattacgcgc agaaaaaaag gatctcaaga agatcctttg
     5641 atcttttcta cggggtctga cgctcagtgg aacgaaaact cacgttaagg gattttggtc
     5701 atgcattcta ggtactaaaa caattcatcc agtaaaatat aatattttat tttctcccaa
     5761 tcaggcttga tccccagtaa gtcaaaaaat agctcgacat actgttcttc cccgatatcc
     5821 tccctgatcg accggacgca gaaggcaatg tcataccact tgtccgccct gccgcttctc
     5881 ccaagatcaa taaagccact tactttgcca tctttcacaa agatgttgct gtctcccagg
     5941 tcgccgtggg aaaagacaag ttcctcttcg ggcttttccg tctttaaaaa atcatacagc
     6001 tcgcgcggat ctttaaatgg agtgtcttct tcccagtttt cgcaatccac atcggccaga
     6061 tcgttattca gtaagtaatc caattcggct aagcggctgt ctaagctatt cgtataggga
     6121 caatccgata tgtcgatgga gtgaaagagc ctgatgcact ccgcatacag ctcgataatc
     6181 ttttcagggc tttgttcatc ttcatactct tccgagcaaa ggacgccatc ggcctcactc
     6241 atgagcagat tgctccagcc atcatgccgt tcaaagtgca ggacctttgg aacaggcagc
     6301 tttccttcca gccatagcat catgtccttt tcccgttcca catcataggt ggtcccttta
     6361 taccggctgt ccgtcatttt taaatatagg ttttcatttt ctcccaccag cttatatacc
     6421 ttagcaggag acattccttc cgtatctttt acgcagcggt atttttcgat cagttttttc
     6481 aattccggtg atattctcat tttagccatt tattatttcc ttcctctttt ctacagtatt
     6541 taaagatacc ccaagaagct aattataaca agacgaactc caattcactg ttccttgcat
     6601 tctaaaacct taaataccag aaaacagctt tttcaaagtt gttttcaaag ttggcgtata
     6661 acatagtatc gacggagccg attttgaaac cgcggtgatc acaggcagca acgctctgtc
     6721 atcgttacaa tcaacatgct accctccgcg agatcatccg tgtttcaaac ccggcagctt
     6781 agttgccgtt cttccgaata gcatcggtaa catgagcaaa gtctgccgcc ttacaacggc
     6841 tctcccgctg acgccgtccc ggactgatgg gctgcctgta tcgagtggtg attttgtgcc
     6901 gagctgccgg tcggggagct gttggctggc tggtggcagg atatattgtg gtgtaaacaa
     6961 attgacgctt agacaactta ataacacatt gcggacgttt ttaatgtact gaattaacgc
     7021 cgaattaatt cgggggatct ggattttagt actggatttt ggttttagga attagaaatt
     7081 ttattgatag aagtatttta caaatacaaa tacatactaa gggtttctta tatgctcaac
     7141 acatgagcga aaccctatag gaaccctaat tcccttatct gggaactact cacacattat
     7201 tatggagaaa ctcgagcttg tcgatcgaca gatccggtcg gcatctactt cagaagaact
     7261 cgtcaagaag gcgatagaag gcgatgcgct gcgaatcggg agcggcgata ccgtaaagca
     7321 cgaggaagcg gtcagcccat tcgccgccaa gctcttcagc aatatcacgg gtagccaacg
     7381 ctatgtcctg atagcggtcc gccacaccca gccggccaca gtcgatgaat ccagaaaagc
     7441 ggccattttc caccatgata ttcggcaagc aggcatcgcc atgtgtcacg acgagatcct
     7501 cgccgtcggg catgcgcgcc ttgagcctgg cgaacagttc ggctggcgcg agcccctgat
     7561 gctcttcgtc cagatcatcc tgatcgacaa gaccggcttc catccgagta cgtgctcgct
     7621 cgatgcgatg tttcgcttgg tggtcgaatg ggcaggtagc cggatcaagc gtatgcagcc
     7681 gccgcattgc atcagccatg atggatactt tctcggcagg agcaaggtga gatgacagga
     7741 gatcctgccc cggcacttcg cccaatagca gccagtccct tcccgcttca gtgacaacgt
     7801 cgagcacagc tgcgcaagga acgcccgtcg tggccagcca cgatagccgc gctgcctcgt
     7861 cctggagttc attcagggca ccggacaggt cggtcttgac aaaaagaacc gggcgcccct
     7921 gcgctgacag ccggaacacg gcggcatcag agcagccgat tgtctgttgt gcccagtcat
     7981 agccgaatag cctctccacc caagcggccg gagaacctgc gtgcaatcca tcttgttcaa
     8041 tccccatatc tcattgcccc ccgggatctg cgaaagctcg agagagatag atttgtagag
     8101 agagactggt gatttcagcg tgtcctctcc aaatgaaatg aacttcctta tatagaggaa
     8161 gggtcttgcg aaggatagtg ggattgtgcg tcatccctta cgtcagtgga gatatcacat
     8221 caatccactt gctttgaaga cgtggttgga acgtcttctt tttccacgat gctcctcgtg
     8281 ggtgggggtc catctttggg accactgtcg gcagaggcat cttgaacgat agcctttcct
     8341 ttatcgcaat gatggcattt gtaggtgcca ccttcctttt ctactgtcct tttgatgaag
     8401 tgacagatag ctgggcaatg gaatccgagg aggtttcccg atattaccct ttgttgaaaa
     8461 gtctcaatag ccctttggtc ttctgagact gtatctttga tattcttgga gtagacgaga
     8521 gtgtcgtgct ccaccatgtt cacatcaatc cacttgcttt gaagacgtgg ttggaacgtc
     8581 ttctttttcc acgatgctcc tcgtgggtgg gggtccatct ttgggaccac tgtcggcaga
     8641 ggcatcttga acgatagcct ttcctttatc gcaatgatgg catttgtagg tgccaccttc
     8701 cttttctact gtccttttga tgaagtgaca gatagctggg caatggaatc cgaggaggtt
     8761 tcccgatatt accctttgtt gaaaagtctc aatagccctt tggtcttctg agactgtatc
     8821 tttgatattc ttggagtaga cgagagtgtc gtgctccacc atgttggcaa gctgctctag
     8881 ccaatacgca aaccgcctct ccccgcgcgt tggccgattc attaatgcag ctggcacgac
     8941 aggtttcccg actggaaagc gggcagtgag cgcaacgcaa ttaatgtgag ttagctcact
     9001 cattaggcac cccaggcttt acactttatg cttccggctc gtatgttgtg tggaattgtg
     9061 agcggataac aatttcacac aggaaacagc tatgacatga ttacgaattc ggggaaattc
     9121 gagctggtca cccttatctt taatcatatt ccatagtcca taccatagca catacagtag
     9181 ttatatgctg gagaagagat ccaacaaaac attcacaatg gattatagaa acatttgttt
     9241 attcattata atgagatgtt acattcattt aatattagaa aaagccacaa attcataaca
     9301 caacaagcca agaaaaaaac acaaacttaa gcacacaacc tttttatttg acacaccaaa
     9361 tatttcatct tcatcttcat attcactatc aggcggaggt gaacttgtag gagccgtggc
     9421 aaagtgtgcc atgatcccag gaggagtgga tcagctcggc cttccacttt tcaccagcag
     9481 cgccgagcac aacgtggagc gggtagaagt gctctggaaa tggatgggcg agcttccagt
     9541 tcggggcctt gctctcgtag atgttcactt cctcgaagcg gccgttgatg agggccttcc
     9601 taagccaaga atcgaaggca gcggcccaag gggcaacacc atcgaagtag tgcggtgtct
     9661 catcgagtgg atgtgtggcg ctgccggagc caataatgag cacgccatca ttcttgagcg
     9721 gcgcgagggc tctgccgaga ttgtagtggt aggtgccgtc gagatgtggc tgaactgaga
     9781 gctggcacac cgggatgtcc gcctctggat acatgagcat gagtggaacc caggcgccgt
     9841 gatcaaggcc cctcttttgg tctgtctcgg ctgtctcgaa cccggacttc ttgaggatct
     9901 cttccacctt tctcgcaagc tctggctcgc ctggagctgg gtacttgaac tggtacatgg
     9961 cggctgggta gtcgtcgaag tcgtggatgg tgtcgttgat gtgcacggcg ttcacggttg
    10021 gcttcactgt ctcccagtgg ccggagatga tgaggatggc cttcggcttc ttggagaaaa
    10081 tcttctcgcg ccatgtctcg aagaacggcc tgagcggatg tgtatcctcg acggtgagga
    10141 tcgggttgcc gtgggtgatg aagaaggact ctttgatcat ctggtcgttg gcgtcctcgc
    10201 cgttcatcat cttcataggt ccagggttct cctccacgtc tccagcctgc ttcagcaggc
    10261 tgaagttagt agctccgctt ccgtagcgcg gaatcgggat gagcttgagc ggcttggtct
    10321 tttggagcgc aatgccgaac ttctcgtcca tgtcgaggtc cttcggggag atgtcgcctt
    10381 cgagcttcca gttgaagaac tggaggagtg tggcgagcat gagggtgagc atcctgatgg
    10441 cgagattcat gcctgggcaa attctgcggc cagcgccaaa tggaaggagg ccgaaatcgc
    10501 ggcccttaac atcgatctcg cagccgatga agcgctctgg gctgaaaata tcggcgttct
    10561 gccaggcgtt tgggtccctg ccaatggccc agaggttcac gaggatctgg gcatccttcg
    10621 gcacgatgta gccgtacagc tcgacatcgg tgtcggcctt tcttgggagc aagaacacgg
    10681 tcggcggatg gaggcggagt gtctctttga tgatcgcctg gaggtacggc aggttgatga
    10741 tgtcggactc ttggatctgc ttgtccttgc cgaggacctg cttgatttcc tcttgggcct
    10801 tttccatcat ctctggattg cggatcagct cggtcatcac ccactcgaat gtggaggatg
    10861 tggtatcggt gccggcgtcg aagatgtcca cgaggaggtg gttgatctcg cccatcgtca
    10921 gctcgttctg cttgaacagc tggaggagca catcgagcac gtcgtcggtg gtggtggtag
    10981 ttgtggtgga ggaatctggg gcgagtctct cgcagatgat gccctggaac acggcgatca
    11041 gcttgtcgaa ggagcaggcg agccttcttc taatgccaga tgggtcaacg cagccgagaa
    11101 tcgggaagta gtcggcgtag ttcggcttgc ctatatcttc catgatgttc cagatcagct
    11161 ctttgaactc ttggctggta tgggacttgt ggtgcgccag ctcgacgctg aagaacagct
    11221 tgctgaggag gttgaggctg gtcgtaaagg cggccttgcc aatatccacg gcctggcctt
    11281 tctgggcgca ctcttgaacg tactcgtaga gctgctgcac cttggcgtgc ctgaatgtct
    11341 ggcaggcatc gagtctctgt ggggagagca gatgcacggc ggtaatcttg cggaagttgc
    11401 gccacttcgg agacaccggg agccaagaca tggtgagctt gtggtggtcg ccggctgtaa
    11461 cactattcgg gatcgtgcgg ttggagagcg ggtgatcctt cttgaggaac atctctttgg
    11521 ccacgtcggc agaggacaca acgatggtgg tcacagagcc gagtctgagg gaaatgagtg
    11581 ggccgtgaat cttggcgagg ttggcgaagg accgatgcgg cttcttgccc acctcaagga
    11641 tgttgccgat gatcggaagc ggctttggtc ctggcggaag cagcttggtg gtctgctggg
    11701 agaacagcag cttgatgaag tggaagctga tgaaccagat cgcgaggatc atggcgaggg
    11761 tcgcatgatc cattctagag tcccccgtgt tctctccaaa tgaaatgaac ttccttatat
    11821 agaggaaggg tcttgcgaag gatagtggga ttgtgcgtca tcccttacgt cagtggagat
    11881 atcacatcaa tccacttgct ttgaagacgt ggttggaacg tcttcttttt ccacgatgct
    11941 cctcgtgggt gggggtccat ctttgggacc actgtcggca gaggcatctt caacgatggc
    12001 ctttccttta tcgcaatgat ggcatttgta ggagccacct tccttttcca ctatcttcac
    12061 aataaagtga cagatagctg ggcaatggaa tccgaggagg tttccggata ttaccctttg
    12121 ttgaaaagtc tcaattgccc tttggtcttc tgagactgta tctttgatat ttttggagta
    12181 gacaagtgtg tcgtgctcca ccatgttgac gaagattttc ttcttgtcat tgagtcgtaa
    12241 gagactctgt atgaactgtt cgccagtctt tacggcgagt tctgttaggt cctctatttg
    12301 aatctttgac tccatggcct ttgattcagt gggaactacc tttttagaga ctccaatctc
    12361 tattacttgc cttggtttgt gaagcaagcc ttgaatcgtc catactggaa tagtacttct
    12421 gatcttgaga aatatatctt tctctgtgtt cttgatgcag ttagtcctga atcttttgac
    12481 tgcatcttta accttcttgg gaaggtattt gatttcctgg agattattgc tcgggtagat
    12541 cgtcttgatg agacctgctg cgtaagcctc tctaaccatc tgtgggttag cattctttct
    12601 gaaattgaaa aggctaatct ggggacctgc aggcatgcaa gcttggcact ggccgtcgtt
    12661 ttacaacgtc gtgactggga aaaccctggc gttacccaac ttaatcgcct tgcagcacat
    12721 ccccctttcg ccagctggcg taatagcgaa gaggcccgca ccgatcgccc ttcccaacag
    12781 ttgcgcagcc tgaatggcga atgctagagc agcttgagct tggatcagat tgtcgtttcc
    12841 cgccttcagt ttagcttcat ggagtcaaag attcaaatag aggacctaac agaactcgcc
    12901 gtaaagactg gcgaacagtt catacagagt ctcttacgac tcaatgacaa gaagaaaatc
    12961 ttcgtcaaca tggtggagca cgacacactt gtctactcca aaaatatcaa agatacagtc
    13021 tcagaagacc aaagggcaat tgagactttt caacaaaggg taatatccgg aaacctcctc
    13081 ggattccatt gcccagctat ctgtcacttt attgtgaaga tagtggaaaa ggaaggtggc
    13141 tcctacaaat gccatcattg cgataaagga aaggccatcg ttgaagatgc ctctgccgac
    13201 agtggtccca aagatggacc cccacccacg aggagcatcg tggaaaaaga agacgttcca
    13261 accacgtctt caaagcaagt ggattgatgt gatatctcca ctgacgtaag ggatgacgca
    13321 caatcccact atccttcgca agacccttcc tctatataag gaagttcatt tcatttggag
    13381 agaacacggg ggactcttga catgaccgcc atcaagatga acaccaacgg cgagggcgag
    13441 acacagcaca tcctcatgat cccgttcatg gcgcagggcc acctcaggcc atttctcgaa
    13501 ctcgccatgt tcctctacaa gcgctcccac gtgatcatca ccctgctcac aactccgctc
    13561 aacgccggct tcctcaggca cctccttcac caccattcct actcctccag cggcatcagg
    13621 atcgtcgagc tgccattcaa ctccaccaac cacggactcc caccgggcat cgagaacacc
    13681 gataagctca cactcccgct cgtggtgtcc ctcttccatt ccaccatcag cctcgatccg
    13741 cacctccgcg attacatctc caggcatttc agcccagcca ggccaccact ctgcgtgatc
    13801 catgatgtgt tcctcggctg ggttgaccag gtggccaagg atgtgggctc tacaggcgtg
    13861 gtgttcacaa caggcggcgc ttatggcaca tccgcctacg tgtccatctg gaacgatctc
    13921 ccgcaccaga actactccga cgaccaagag ttcccgctgc caggcttccc agagaaccat
    13981 aagttccgca ggtcccagct ccatcggttc ctcagatatg ccgacggctc cgacgattgg
    14041 tccaagtatt tccagccgca gctccgccag tccatgaagt cttttggctg gctctgcttg
    14101 tcctacgaga ccgagattct tactgttgag tatggattgc ttccaatcgg caagatcgta
    14161 gagaaacgta ttgagtgtac agtttattct gttgacaaca acggaaatat ctatactcag
    14221 cctgttgctc aatggcacga tagaggggaa caagaggttt tcgagtattg ccttgaggat
    14281 ggtagcctca ttcgagcaac aaaagatcac aaatttatga cagtggatgg tcagatgtta
    14341 cctatcgatg aaattttcga aagggaatta gacctcatga gagtggataa cctccctaat
    14401 tagatatgaa gatgaagatg aaatatttgg tgtgtcaaat aaaaagcttg tgtgcttaag
    14461 tttgtgtttt tttcttggct tgttgtgtta tgaatttgtg gctttttcta atattaaatg
    14521 aatgtaagat ctcattataa tgaataaaca aatgtttcta taatccattg tgaatgtttt
    14581 gttggatctc ttctgcagca tataactact gtatgtgcta tggtatggac tatggaatat
    14641 gattaaagat aagatgggct catagagtaa aacgaggcga gggacctata aacctccctt
    14701 catcatgcta tttcatgatc tattttataa aataaagatg tagaaaaaag taagcgtaat
    14761 aaccgcaaaa caaatgattt aaaacatggc acataatgag gagattaagt tcggtttacg
    14821 tttattttag tactaattgt aacgtgagac tacgtatcgg gaatcgccta attaaagcat
    14881 taatgcgaac ctgattagat tcaccgaccc tcctatcgtg tcgacctttc tgtttcttag
    14941 aattttttgg tagtctatgt actaataatg tcagcttcgt atttatttca taagcaattt
    15001 gcatttgcaa tttgtttttt acttttattt ttattgtatt gtggaatgtg gactcgtacc
    15061 aacatgaagt tatataccac caaaaaaatt acagttagtc aaaagattca cgagtgagag
    15121 ctacttatga ttgtctttta cgtatatgtc taattgtcta tttgctcaat aatctttgta
    15181 ctttcttttg tcgttgataa aatcacaaag ttccaaaagt aatcgaatga tttgctttta
    15241 agaaaagaag agctcaataa ttcaacatat atctgtacac aacgcg
//
