LOCUS       sequence-410746-       14397 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  NpuDnaE(C)-Glucos(232-500).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 14397)
  AUTHORS   Yuan G, Lu H, De K, Hassan MM, Liu Y, Islam MT, Muchero W, Tuskan 
            GA, Yang X
  TITLE     Split selectable marker systems utilizing inteins facilitate gene 
            stacking in plants.
  JOURNAL   Commun Biol. 2023 May 26;6(1):567. doi: 10.1038/s42003-023-04950-8.
  PUBMED    37237044
REFERENCE   2  (bases 1 to 14397)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Commun 
            Biol."; date: "2023-05-26"; volume: "6(1):567. doi"; pages: " 
            10.1038/s42003-023-04950-8"
COMMENT     Sequence Label: pAXY0007
FEATURES             Location/Qualifiers
     source          1..14397
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        complement(16..361)
                     /label=CaMV 35S promoter
                     /note="strong constitutive promoter from cauliflower mosaic
                     virus"
     primer_bind     723..745
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     737..754
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     738..754
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        1269..1614
                     /label=CaMV 35S promoter
                     /note="strong constitutive promoter from cauliflower mosaic
                     virus"
     terminator      2664..2912
                     /label=HSP terminator
                     /note="efficient transcription terminator from the 
                     Arabidopsis thaliana heat shock protein 18.2 gene (Nagaya 
                     et al., 2010)"
     primer_bind     complement(3602..3618)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(3602..3618)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(3615..3637)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    3626..3642
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(3650..3656,3657..3674,3675..3680))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    3695..3716
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        3907..4583
                     /label=CaMV 35S promoter (enhanced)
                     /note="cauliflower mosaic virus 35S promoter with a 
                     duplicated enhancer region"
     CDS             4651..5448
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MGIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGR
                     PVLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLL
                     SSHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEH
                     QGLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDI
                     ALATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(4708..4727)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     5318..5337
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     polyA_signal    5488..5662
                     /label=CaMV poly(A) signal
                     /note="cauliflower mosaic virus polyadenylation signal"
     misc_feature    5740..5764
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     CDS             6189..6983
                     /codon_start=1
                     /gene="aphA-3"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin"
                     /translation="MAKMRISPELKKLIEKYRCVKDTEGMSPAKVYKLVGENENLYLKM
                     TDSRYKGTTYDVEREKDMMLWLEGKLPVPKVLHFERHDGWSNLLMSEADGVLCSEEYED
                     EQSPEKIIELYAECIRLFHSIDISDCPYTNSLDSRLAELDYLLNNDLADVDCENWEEDT
                     PFKDPRELYDFLKTEKPEEELVFSHGDLGDSNIFVKDGKVSGFIDLGRSGRADKWYDIA
                     FCVRSIREDIGEEQYVELFFDLLGIKPDWEKIKYYILLDELF"
     rep_origin      7070..7658
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     7559..7578
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     7812..7829
                     /label=L4440
                     /note="L4440 vector, forward primer"
     misc_feature    7844..7984
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     complement(7910..7929)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     8070..8092
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     rep_origin      8328..8522
                     /label=pVS1 oriV
                     /note="origin of replication for the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
     CDS             complement(8588..9661)
                     /codon_start=1
                     /product="replication protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 RepA
                     /translation="MSGRKPSGPVQIGAALGDDLVEKLKAAQAAQRQRIEAEARPGESW
                     QAAADRIRKESRQPPAAGAPSIRKPPKGDEQPDFFVPMLYDVGTRDSRSIMDVAVFRLS
                     KRDRRAGEVIRYELPDGHVEVSAGPAGMASVWDYDLVLMAVSHLTESMNRYREGKGDKP
                     GRVFRPHVADVLKFCRRADGGKQKDDLVETCIRLNTTHVAMQRTKKAKNGRLVTVSEGE
                     ALISRYKIVKSETGRPEYIEIELADWMYREITEGKNPDVLTVHPDYFLIDPGIGRFLYR
                     LARRAAGKAEARWLFKTIYERSGSAGEFKKFCFTVRKLIGSNDLPEYDLKEEAGQAGPI
                     LVMRYRNLIEGEASAGS"
     CDS             complement(10090..10719)
                     /codon_start=1
                     /product="stability protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 StaA
                     /translation="MKVIAVLNQKGGSGKTTIATHLARALQLAGADVLLVDSDPQGSAR
                     DWAAVREDQPLTVVGIDRPTIDRDVKAIGRRDFVVIDGAPQAADLAVSAIKAADFVLIP
                     VQPSPYDIWATADLVELVKQRIEVTDGRLQAAFVVSRAIKGTRIGGEVAEALAGYELPI
                     LESRITQRVSYPGTAAAGTTVLESEPEGDAAREVQALAAEIKSKLI"
     misc_feature    12020..12044
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     terminator      complement(12066..12318)
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     CDS             complement(12350..12367)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             complement(join(12374..14191,14382..14396))
                     /codon_start=1
                     /product="beta-glucuronidase"
                     /label=GUSPlus(TM)
                     /note="codon-optimized Staphylococcus gusA gene with a 
                     catalase intron to ensure expression in plants but not 
                     bacteria"
                     /translation="MVDLRNRRTSLYPINTETRGVFDLNGVWNFKLDYGKGLEEKWYES
                     KLTDTISMAVPSSYNDIGVTKEIRNHIGYVWYEREFTVPAYLKDQRIVLRFGSATHKAI
                     VYVNGELVVEHKGGFLPFEAEINNSLRDGMNRVTVAVDNILDDSTLPVGLYSERHEEGL
                     GKVIRNKPNFDFFNYAGLHRPVKIYTTPFTYVEDISVVTDFNGPTGTVTYTVDFQGKAE
                     TVKVSVVDEEGKVVASTEGLSGNVEIPNVILWEPLNTYLYQIKVELVNDGLTIDVYEEP
                     FGVRTVEVNDGKFLINNKPFYFKGFGKHEDTPINGRGFNEASNVMDFNILKWIGANSFR
                     TAHYPYSEELMRLADREGLVVIDETPAVGVHLNFMATTGLGEGSERVSTWEKIRTFEHH
                     QDVLRELVSRDKNHPSVVMWSIANEAATEEEGAYEYFKPLVELTKELDPQKRPVTIVLF
                     VMATPETDKVAELIDVIALNRYNGWYFDGGDLEAAKVHLRQEFHAWNKRCPGKPIMITE
                     YGADTVAGFHDIDPVMFTEEYQVEYYQANHVVFDEFENFVGEQAWNFADFATSQGVMRV
                     QGNKKGVFTRDRKPKLAAHVFRERWTNIPDFGYKN"
     intron          14192..14381
                     /label=cat1 intron
                     /note="castor bean catalase intron, modified"
ORIGIN
        1 gtcaagagtc ccccgtgttc tctccaaatg aaatgaactt ccttatatag aggaagggtc
       61 ttgcgaagga tagtgggatt gtgcgtcatc ccttacgtca gtggagatat cacatcaatc
      121 cacttgcttt gaagacgtgg ttggaacgtc ttctttttcc acgatgctcc tcgtgggtgg
      181 gggtccatct ttgggaccac tgtcggcaga ggcatcttca acgatggcct ttcctttatc
      241 gcaatgatgg catttgtagg agccaccttc cttttccact atcttcacaa taaagtgaca
      301 gatagctggg caatggaatc cgaggaggtt tccggatatt accctttgtt gaaaagtctc
      361 aattgccctt tggtcttctg agactgtatc tttgatattt ttggagtaga caagtgtgtc
      421 gtgctccacc atgttgacga agattttctt cttgtcattg agtcgtaaga gactctgtat
      481 gaactgttcg ccagtcttta cggcgagttc tgttaggtcc tctatttgaa tctttgactc
      541 catgaagcta aactgaaggc gggaaacgac aatctgatcc aagctcaagc tgctctagca
      601 ttcgccattc aggctgcgca actgttggga agggcgatcg gtgcgggcct cttcgctatt
      661 acgccagctg gcgaaagggg gatgtgctgc aaggcgatta agttgggtaa cgccagggtt
      721 ttcccagtca cgacgttgta aaacgacggc cagtgccaag cttgcatgcc tgcaggtccc
      781 cagattagcc ttttcaattt cagaaagaat gctaacccac agatggttag agaggcttac
      841 gcagcaggtc tcatcaagac gatctacccg agcaataatc tccaggaaat caaatacctt
      901 cccaagaagg ttaaagatgc agtcaaaaga ttcaggacta actgcatcaa gaacacagag
      961 aaagatatat ttctcaagat cagaagtact attccagtat ggacgattca aggcttgctt
     1021 cacaaaccaa ggcaagtaat agagattgga gtctctaaaa aggtagttcc cactgaatca
     1081 aaggccatgg agtcaaagat tcaaatagag gacctaacag aactcgccgt aaagactggc
     1141 gaacagttca tacagagtct cttacgactc aatgacaaga agaaaatctt cgtcaacatg
     1201 gtggagcacg acacacttgt ctactccaaa aatatcaaag atacagtctc agaagaccaa
     1261 agggcaattg agacttttca acaaagggta atatccggaa acctcctcgg attccattgc
     1321 ccagctatct gtcactttat tgtgaagata gtggaaaagg aaggtggctc ctacaaatgc
     1381 catcattgcg ataaaggaaa ggccatcgtt gaagatgcct ctgccgacag tggtcccaaa
     1441 gatggacccc cacccacgag gagcatcgtg gaaaaagaag acgttccaac cacgtcttca
     1501 aagcaagtgg attgatgtga tatctccact gacgtaaggg atgacgcaca atcccactat
     1561 ccttcgcaag acccttcctc tatataagga agttcatttc atttggagag aacacggggg
     1621 actcaaacat tactcattca caaaaccatc ttaaagcaac tacacaagtc ttgaaatttt
     1681 ctcatatttt ctatttacta tataaacttt taatcaaatc aagattaaag ttaattaaat
     1741 gattaagatt gcgacccgta agtacctggg gaagcaaaac gtttacgata tcggtgtgga
     1801 gagagatcac aattttgctc tgaaaaatgg attcatagct tctaattgca actccgtgga
     1861 agagatcgag acactcggct tctccatcct ccgcaactac accaagctgc cgatctgggg
     1921 catcggccca cttattgctt ccccagtgca gcactcctcc tccgacaaca attcaacagg
     1981 cgccgagttc gtgcagtggc tcagcctcaa agagccggac tccgtcctct acatctcctt
     2041 cggctcccag aacacgatca gcccgacgca gatgatggaa ctcgctgctg gccttgagtc
     2101 ctccgagaag ccattcctct gggtgatcag agccccgttc ggcttcgaca tcaacgaaga
     2161 gatgcgccca gagtggctgc cagagggctt tgaggaacgc atgaaggtga agaaacaggg
     2221 caagctcgtg tacaagctcg gcccgcagct tgagatcctc aaccatgaat ccatcggcgg
     2281 ctttctcacc cactgcggat ggaacagcat ccttgagtct cttcgcgagg gcgttccgat
     2341 gcttggatgg ccacttgctg ccgagcaggc ctacaacctc aagtacctcg aagatgagat
     2401 gggcgtcgcg gttgagcttg ctagaggcct cgaaggcgag atctccaaag agaaggtcaa
     2461 gcgcatcgtc gagatgatcc ttgagcgcaa cgagggctcc aaaggctggg agatgaagaa
     2521 tcgcgccgtg gaaatgggca aaaagctcaa ggacgccgtg aacgaggaaa aagagctgaa
     2581 gggctcctcc gtgaaggcga tcgacgattt cctcgacgcc gtcatgcagg ccaaacttga
     2641 gccaagcctc cagtgatagt gaatatgaag atgaagatga aatatttggt gtgtcaaata
     2701 aaaagcttgt gtgcttaagt ttgtgttttt ttcttggctt gttgtgttat gaatttgtgg
     2761 ctttttctaa tattaaatga atgtaagatc tcattataat gaataaacaa atgtttctat
     2821 aatccattgt gaatgttttg ttggatctct tctgcagcat ataactactg tatgtgctat
     2881 ggtatggact atggaatatg attaaagata agatgggctc atagagtaaa acgaggcgag
     2941 ggacctataa acctcccttc atcatgctat ttcatgatct attttataaa ataaagatgt
     3001 agaaaaaagt aagcgtaata accgcaaaac aaatgattta aaacatggca cataatgagg
     3061 agattaagtt cggtttacgt ttattttagt actaattgta acgtgagact acgtatcggg
     3121 aatcgcctaa ttaaagcatt aatgcgaacc tgattagatt caccgaccct cctatcgtgt
     3181 cgacctttct gtttcttaga attttttggt agtctatgta ctaataatgt cagcttcgta
     3241 tttatttcat aagcaatttg catttgcaat ttgtttttta cttttatttt tattgtattg
     3301 tggaatgtgg actcgtacca acatgaagtt atataccacc aaaaaaatta cagttagtca
     3361 aaagattcac gagtgagagc tacttatgat tgtcttttac gtatatgtct aattgtctat
     3421 ttgctcaata atctttgtac tttcttttgt cgttgataaa atcacaaagt tccaaaagta
     3481 atcgaatgat ttgcttttaa gaaaagaaga gctcaataat tcaacatata tctgtacaca
     3541 attaccgcct ttgagtgagc tctagatgcc acctgacgtc taagaagaat tcgtaatcat
     3601 ggtcatagct gtttcctgtg tgaaattgtt atccgctcac aattccacac aacatacgag
     3661 ccggaagcat aaagtgtaaa gcctggggtg cctaatgagt gagctaactc acattaattg
     3721 cgttgcgctc actgcccgct ttccagtcgg gaaacctgtc gtgccagctg cattaatgaa
     3781 tcggccaacg cgcggggaga ggcggtttgc gtattggcta gagcagcttg ccaacatggt
     3841 ggagcacgac actctcgtct actccaagaa tatcaaagat acagtctcag aagaccaaag
     3901 ggctattgag acttttcaac aaagggtaat atcgggaaac ctcctcggat tccattgccc
     3961 agctatctgt cacttcatca aaaggacagt agaaaaggaa ggtggcacct acaaatgcca
     4021 tcattgcgat aaaggaaagg ctatcgttca agatgcctct gccgacagtg gtcccaaaga
     4081 tggaccccca cccacgagga gcatcgtgga aaaagaagac gttccaacca cgtcttcaaa
     4141 gcaagtggat tgatgtgaac atggtggagc acgacactct cgtctactcc aagaatatca
     4201 aagatacagt ctcagaagac caaagggcta ttgagacttt tcaacaaagg gtaatatcgg
     4261 gaaacctcct cggattccat tgcccagcta tctgtcactt catcaaaagg acagtagaaa
     4321 aggaaggtgg cacctacaaa tgccatcatt gcgataaagg aaaggctatc gttcaagatg
     4381 cctctgccga cagtggtccc aaagatggac ccccacccac gaggagcatc gtggaaaaag
     4441 aagacgttcc aaccacgtct tcaaagcaag tggattgatg tgatatctcc actgacgtaa
     4501 gggatgacgc acaatcccac tatccttcgc aagacccttc ctctatataa ggaagttcat
     4561 ttcatttgga gaggacacgc tgaaatcacc agtctctctc tacaaatcta tctctctcga
     4621 gctttcgcag atcccggggg gcaatgagat atggggattg aacaagatgg attgcacgca
     4681 ggttctccgg ccgcttgggt ggagaggcta ttcggctatg actgggcaca acagacaatc
     4741 ggctgctctg atgccgccgt gttccggctg tcagcgcagg ggcgcccggt tctttttgtc
     4801 aagaccgacc tgtccggtgc cctgaatgaa ctccaggacg aggcagcgcg gctatcgtgg
     4861 ctggccacga cgggcgttcc ttgcgcagct gtgctcgacg ttgtcactga agcgggaagg
     4921 gactggctgc tattgggcga agtgccgggg caggatctcc tgtcatctca ccttgctcct
     4981 gccgagaaag tatccatcat ggctgatgca atgcggcggc tgcatacgct tgatccggct
     5041 acctgcccat tcgaccacca agcgaaacat cgcatcgagc gagcacgtac tcggatggaa
     5101 gccggtcttg tcgatcagga tgatctggac gaagagcatc aggggctcgc gccagccgaa
     5161 ctgttcgcca ggctcaaggc gcgcatgccc gacggcgagg atctcgtcgt gacacatggc
     5221 gatgcctgct tgccgaatat catggtggaa aatggccgct tttctggatt catcgactgt
     5281 ggccggctgg gtgtggcgga ccgctatcag gacatagcgt tggctacccg tgatattgct
     5341 gaagagcttg gcggcgaatg ggctgaccgc ttcctcgtgc tttacggtat cgccgctccc
     5401 gattcgcagc gcatcgcctt ctatcgcctt cttgacgagt tcttctgaag tagatgccga
     5461 ccggatctgt cgatcgacaa gctcgagttt ctccataata atgtgtgagt agttcccaga
     5521 taagggaatt agggttccta tagggtttcg ctcatgtgtt gagcatataa gaaaccctta
     5581 gtatgtattt gtatttgtaa aatacttcta tcaataaaat ttctaattcc taaaaccaaa
     5641 atccagtact aaaatccaga tcccccgaat taattcggcg ttaattcagt acattaaaaa
     5701 cgtccgcaat gtgttattaa gttgtctaag cgtcaatttg tttacaccac aatatatcct
     5761 gccaccagcc agccaacagc tccccgaccg gcagctcggc acaaaatcac cactcgatac
     5821 aggcagccca tcagtccggg acggcgtcag cgggagagcc gttgtaaggc ggcagacttt
     5881 gctcatgtta ccgatgctat tcggaagaac ggcaactaag ctgccgggtt tgaaacacgg
     5941 atgatctcgc ggagggtagc atgttgattg taacgatgac agagcgttgc tgcctgtgat
     6001 caccgcggtt tcaaaatcgg ctccgtcgat actatgttat acgccaactt tgaaaacaac
     6061 tttgaaaaag ctgttttctg gtatttaagg ttttagaatg caaggaacag tgaattggag
     6121 ttcgtcttgt tataattagc ttcttggggt atctttaaat actgtagaaa agaggaagga
     6181 aataataaat ggctaaaatg agaatatcac cggaattgaa aaaactgatc gaaaaatacc
     6241 gctgcgtaaa agatacggaa ggaatgtctc ctgctaaggt atataagctg gtgggagaaa
     6301 atgaaaacct atatttaaaa atgacggaca gccggtataa agggaccacc tatgatgtgg
     6361 aacgggaaaa ggacatgatg ctatggctgg aaggaaagct gcctgttcca aaggtcctgc
     6421 actttgaacg gcatgatggc tggagcaatc tgctcatgag tgaggccgat ggcgtccttt
     6481 gctcggaaga gtatgaagat gaacaaagcc ctgaaaagat tatcgagctg tatgcggagt
     6541 gcatcaggct ctttcactcc atcgacatat cggattgtcc ctatacgaat agcttagaca
     6601 gccgcttagc cgaattggat tacttactga ataacgatct ggccgatgtg gattgcgaaa
     6661 actgggaaga agacactcca tttaaagatc cgcgcgagct gtatgatttt ttaaagacgg
     6721 aaaagcccga agaggaactt gtcttttccc acggcgacct gggagacagc aacatctttg
     6781 tgaaagatgg caaagtaagt ggctttattg atcttgggag aagcggcagg gcggacaagt
     6841 ggtatgacat tgccttctgc gtccggtcga tcagggagga tatcggggaa gaacagtatg
     6901 tcgagctatt ttttgactta ctggggatca agcctgattg ggagaaaata aaatattata
     6961 ttttactgga tgaattgttt tagtacctag aatgcatgac caaaatccct taacgtgagt
     7021 tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt
     7081 tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt
     7141 gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc
     7201 agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg
     7261 tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg
     7321 ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt
     7381 cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac
     7441 tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg
     7501 acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg
     7561 gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat
     7621 ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt
     7681 tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg
     7741 attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa
     7801 cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc
     7861 tccttacgca tctgtgcggt atttcacacc gcatatggtg cactctcagt acaatctgct
     7921 ctgatgccgc atagttaagc cagtatacac tccgctatcg ctacgtgact gggtcatggc
     7981 tgcgccccga cacccgccaa cacccgctga cgcgccctga cgggcttgtc tgctcccggc
     8041 atccgcttac agacaagctg tgaccgtctc cgggagctgc atgtgtcaga ggttttcacc
     8101 gtcatcaccg aaacgcgcga ggcagggtgc cttgatgtgg gcgccggcgg tcgagtggcg
     8161 acggcgcggc ttgtccgcgc cctggtagat tgcctggccg taggccagcc atttttgagc
     8221 ggccagcggc cgcgataggc cgacgcgaag cggcggggcg tagggagcgc agcgaccgaa
     8281 gggtaggcgc tttttgcagc tcttcggctg tgcgctggcc agacagttat gcacaggcca
     8341 ggcgggtttt aagagtttta ataagtttta aagagtttta ggcggaaaaa tcgccttttt
     8401 tctcttttat atcagtcact tacatgtgtg accggttccc aatgtacggc tttgggttcc
     8461 caatgtacgg gttccggttc ccaatgtacg gctttgggtt cccaatgtac gtgctatcca
     8521 caggaaagag accttttcga cctttttccc ctgctagggc aatttgccct agcatctgct
     8581 ccgtacatta ggaaccggcg gatgcttcgc cctcgatcag gttgcggtag cgcatgacta
     8641 ggatcgggcc agcctgcccc gcctcctcct tcaaatcgta ctccggcagg tcatttgacc
     8701 cgatcagctt gcgcacggtg aaacagaact tcttgaactc tccggcgctg ccactgcgtt
     8761 cgtagatcgt cttgaacaac catctggctt ctgccttgcc tgcggcgcgg cgtgccaggc
     8821 ggtagagaaa acggccgatg ccgggatcga tcaaaaagta atcggggtga accgtcagca
     8881 cgtccgggtt cttgccttct gtgatctcgc ggtacatcca atcagctagc tcgatctcga
     8941 tgtactccgg ccgcccggtt tcgctcttta cgatcttgta gcggctaatc aaggcttcac
     9001 cctcggatac cgtcaccagg cggccgttct tggccttctt cgtacgctgc atggcaacgt
     9061 gcgtggtgtt taaccgaatg caggtttcta ccaggtcgtc tttctgcttt ccgccatcgg
     9121 ctcgccggca gaacttgagt acgtccgcaa cgtgtggacg gaacacgcgg ccgggcttgt
     9181 ctcccttccc ttcccggtat cggttcatgg attcggttag atgggaaacc gccatcagta
     9241 ccaggtcgta atcccacaca ctggccatgc cggccggccc tgcggaaacc tctacgtgcc
     9301 cgtctggaag ctcgtagcgg atcacctcgc cagctcgtcg gtcacgcttc gacagacgga
     9361 aaacggccac gtccatgatg ctgcgactat cgcgggtgcc cacgtcatag agcatcggaa
     9421 cgaaaaaatc tggttgctcg tcgcccttgg gcggcttcct aatcgacggc gcaccggctg
     9481 ccggcggttg ccgggattct ttgcggattc gatcagcggc cgcttgccac gattcaccgg
     9541 ggcgtgcttc tgcctcgatg cgttgccgct gggcggcctg cgcggccttc aacttctcca
     9601 ccaggtcatc acccagcgcc gcgccgattt gtaccgggcc ggatggtttg cgaccgctca
     9661 cgccgattcc tcgggcttgg gggttccagt gccattgcag ggccggcagg caacccagcc
     9721 gcttacgcct ggccaaccgc ccgttcctcc acacatgggg cattccacgg cgtcggtgcc
     9781 tggttgttct tgattttcca tgccgcctcc tttagccgct aaaattcatc tactcattta
     9841 ttcatttgct catttactct ggtagctgcg cgatgtattc agatagcagc tcggtaatgg
     9901 tcttgccttg gcgtaccgcg tacatcttca gcttggtgtg atcctccgcc ggcaactgaa
     9961 agttgacccg cttcatggct ggcgtgtctg ccaggctggc caacgttgca gccttgctgc
    10021 tgcgtgcgct cggacggccg gcacttagcg tgtttgtgct tttgctcatt ttctctttac
    10081 ctcattaact caaatgagtt ttgatttaat ttcagcggcc agcgcctgga cctcgcgggc
    10141 agcgtcgccc tcgggttctg attcaagaac ggttgtgccg gcggcggcag tgcctgggta
    10201 gctcacgcgc tgcgtgatac gggactcaag aatgggcagc tcgtacccgg ccagcgcctc
    10261 ggcaacctca ccgccgatgc gcgtgccttt gatcgcccgc gacacgacaa aggccgcttg
    10321 tagccttcca tccgtgacct caatgcgctg cttaaccagc tccaccaggt cggcggtggc
    10381 ccatatgtcg taagggcttg gctgcaccgg aatcagcacg aagtcggctg ccttgatcgc
    10441 ggacacagcc aagtccgccg cctggggcgc tccgtcgatc actacgaagt cgcgccggcc
    10501 gatggccttc acgtcgcggt caatcgtcgg gcggtcgatg ccgacaacgg ttagcggttg
    10561 atcttcccgc acggccgccc aatcgcgggc actgccctgg ggatcggaat cgactaacag
    10621 aacatcggcc ccggcgagtt gcagggcgcg ggctagatgg gttgcgatgg tcgtcttgcc
    10681 tgacccgcct ttctggttaa gtacagcgat aaccttcatg cgttcccctt gcgtatttgt
    10741 ttatttactc atcgcatcat atacgcagcg accgcatgac gcaagctgtt ttactcaaat
    10801 acacatcacc tttttagacg gcggcgctcg gtttcttcag cggccaagct ggccggccag
    10861 gccgccagct tggcatcaga caaaccggcc aggatttcat gcagccgcac ggttgagacg
    10921 tgcgcgggcg gctcgaacac gtacccggcc gcgatcatct ccgcctcgat ctcttcggta
    10981 atgaaaaacg gttcgtcctg gccgtcctgg tgcggtttca tgcttgttcc tcttggcgtt
    11041 cattctcggc ggccgccagg gcgtcggcct cggtcaatgc gtcctcacgg aaggcaccgc
    11101 gccgcctggc ctcggtgggc gtcacttcct cgctgcgctc aagtgcgcgg tacagggtcg
    11161 agcgatgcac gccaagcagt gcagccgcct ctttcacggt gcggccttcc tggtcgatca
    11221 gctcgcgggc gtgcgcgatc tgtgccgggg tgagggtagg gcgggggcca aacttcacgc
    11281 ctcgggcctt ggcggcctcg cgcccgctcc gggtgcggtc gatgattagg gaacgctcga
    11341 actcggcaat gccggcgaac acggtcaaca ccatgcggcc ggccggcgtg gtggtgtcgg
    11401 cccacggctc tgccaggcta cgcaggcccg cgccggcctc ctggatgcgc tcggcaatgt
    11461 ccagtaggtc gcgggtgctg cgggccaggc ggtctagcct ggtcactgtc acaacgtcgc
    11521 cagggcgtag gtggtcaagc atcctggcca gctccgggcg gtcgcgcctg gtgccggtga
    11581 tcttctcgga aaacagcttg gtgcagccgg ccgcgtgcag ttcggcccgt tggttggtca
    11641 agtcctggtc gtcggtgctg acgcgggcat agcccagcag gccagcggcg gcgctcttgt
    11701 tcatggcgta atgtctccgg ttctagtcgc aagtattcta ctttatgcga ctaaaacacg
    11761 cgacaagaaa acgccaggaa aagggcaggg cggcagcctg tcgcgtaact taggacttgt
    11821 gcgacatgtc gttttcagaa gacggctgca ctgaacgtca gaagccgact gcactatagc
    11881 agcggagggg ttggatcaaa gtactttgat cccgagggga accctgtggt tggcatgcac
    11941 atacaaatgg acgaacggat aaaccttttc acgccctttt aaatatccga ttattctaat
    12001 aaacgctctt ttctcttagg tttacccgcc aatatatcct gtcaaacact gatagtttaa
    12061 ttcccgatct agtaacatag atgacaccgc gcgcgataat ttatcctagt ttgcgcgcta
    12121 tattttgttt tctatcgcgt attaaatgta taattgcggg actctaatca taaaaaccca
    12181 tctcataaat aacgtcatgc attacatgtt aattattaca tgcttaacgt aattcaacag
    12241 aaattatatg ataatcatcg caagaccggc aacaggattc aatcttaaga aactttattg
    12301 ccaaatgttt gaacgatcgg ggaaattcga gctggtcacc aattcacacg tgatggtgat
    12361 ggtgatggct agcgttcttg tagccgaaat ctggaatgtt ggtccagcgc tcgcgaaaga
    12421 cgtgcgcggc gagcttcggc ttgcggtcac gagtgaacac gcccttcttg tttccttgga
    12481 cgcgcatcac gccctgagag gtcgcgaagt ccgcgaagtt ccacgcttgc tcacccacga
    12541 agttctcaaa ctcatcgaac acgacgtggt tcgcctggta gtactcgact tgatattcct
    12601 cggtgaacat cactggatca atgtcgtgaa agcccgcaac ggtgtctgcg ccgtactcag
    12661 tgatcatgat cggctttcct gggcaacgct tgttccacgc gtgaaattcc tggcggagat
    12721 ggactttggc cgcttcgaga tcaccgccat cgaagtacca tccgttatag cgattgagcg
    12781 cgatgacgtc aatcagttcg gcgactttgt ccgtctccgg ggtagccatc acaaacagca
    12841 cgatcgtgac cggacgcttc tgtgggtcga gttccttggt cagctccacc aacggcttga
    12901 agtactcgta cgcgccctct tcctcagtcg ccgcctcgtt ggcgatgctc cacatcacga
    12961 cgcttggatg gttcttgtca cgagacacca gttcacggag aacgtcttga tggtgctcaa
    13021 acgtccgaat cttctcccag gtactgacgc gctcgctgcc ttcgccgagt cccgtggtgg
    13081 ccatgaagtt gaggtgcacg ccaactgccg gagtctcgtc gatcacgacc agaccctcgc
    13141 gatccgcaag acgcatcaac tcttcagagt acggatagtg tgcggtccgg aagctgttgg
    13201 cgccgatcca tttgaggata ttgaaatcca tcacattgct cgcttcgtta aagccacggc
    13261 cgttgatagg agtgtcctca tgtttgccaa agcccttgaa gtagaacggt ttgttgttga
    13321 tgaggaactt gccgtcgttg acttccacgg tccgcacgcc gaacggctct tcatagacat
    13381 cgatggtcag tccgtcgttc accagttcca ctttgatctg gtagagatac gtgttcagtg
    13441 gttcccagag gatgacattc ggaatctcca cgttaccgct caggccctcg gtgcttgcga
    13501 ccactttgcc ttcctcatcc acgaccgaca ctttcacggt ctcggctttg ccttgaaagt
    13561 ccaccgtata ggtcacagtc ccggttgggc cattgaagtc ggtcacaacc gagatgtcct
    13621 cgacgtacgt aaacggggtc gtgtagattt tcaccggacg gtgcaggcct gcatagttga
    13681 agaagtcgaa gttcggcttg ttacgaatga cttttccgag gccctcttcg tggcgctcgc
    13741 tgtacagccc caccgggagg gtgctatcgt cgaggatgtt gtccacggcg acggtgacgc
    13801 gattcatgcc atcacgcagc gagttgttga tttccgcttc gaatggcagg aatccgccct
    13861 tgtgctccac gaccagctca ccattgacat agacaattgc tttgtgagtt gcagagccga
    13921 agcggagcac gatacgctga tccttcagat aggccggcac cgtgaactca cgttcgtacc
    13981 agacatatcc gatatggttg cggatttcct tggtcacgcc aatgtcattg taactgcttg
    14041 ggacggccat actaatagtg tcggtcagct tgctttcgta ccacttctct tccagtcctt
    14101 tcccgtagtc cagcttgaag ttccagacgc cattgaggtc gaagacgcca cgggtctcgg
    14161 tgttgatcgg gtacagacta gttcgtcggt tctgtaacta tcatcatcat catagacaca
    14221 cgaaataaag taatcagatt atcagttaaa gctatgtaat atttacacca taaccaatca
    14281 attaaaaaat agatcagttt aaagaaagat caaagctcaa aaaaataaaa agagaaaagg
    14341 gtcctaacca agaaaatgaa ggagaaaaac tagaaattta ccctcagatc taccatg
//
