LOCUS       sequence-410747-        5706 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  LAMP1 (Lysosome Membrane) labelled with near-infrared fluorescent 
            protein miRFP704nano.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5706)
  AUTHORS   Oliinyk OS, Pletnev S, Baloban M, Verkhusha VV
  TITLE     Development of bright red-shifted miRFP704nano using structural 
            analysis of miRFPnano proteins.
  JOURNAL   Protein Sci. 2023 Jun 22:e4709. doi: 10.1002/pro.4709.
  PUBMED    37347539
REFERENCE   2  (bases 1 to 5706)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Protein 
            Sci. 2023 Jun 22:e4709. doi: 10.1002/pro.4709."
COMMENT     Sequence Label: pLAMP1-miRFP704nano
FEATURES             Location/Qualifiers
     source          1..5706
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        136..327
                     /label=SV40 enhancer
                     /note="enhancer for the SV40 early promoter (Herr, 1993)"
     primer_bind     284..304
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        complement(329..433)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      460..915
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(547..566)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     757..778
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     polyA_signal    922..1043
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(933..952)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     987..1006
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     CDS             complement(join(1691..2848,2849..2911))
                     /codon_start=1
                     /product="rat lysosome-associated membrane glycoprotein 1"
                     /label=LAMP1
                     /translation="MAAPGARRPLLLLLLAGLAHSAPALFEVKDNNGTACIMASFSASF
                     LTTYEAGHVSKVSNMTLPASAEVLKNSSSCGEKNASEPTLAITFGEGYLLKLTFTKNTT
                     RYSVQHMYFTYNLSDTQFFPNASSKGPDTVDSTTDIKADINKTYRCVSDIRVYMKNVTI
                     VLWDATIQAYLPSSNFSKEETRCPQDQPSPTTGPPSPSPPLVPTNPSVSKYNVTGDNGT
                     CLLASMALQLNITYMKKDNTTVTRAFNINPSDKYSGTCGAQLVTLKVGNKSRVLELQFG
                     MNATSSLFFLQGVQLNMTLPDAIEPTFSTSNYSLKALQASVGNSYKCNSEEHIFVSKAL
                     ALNVFSVQVQAFRVESDRFGSVEECVQDGNNMLIPIAVGGALAGLVLIVLIAYLIGRKR
                     SHAGYQTI"
     regulatory      2904..2913
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     regulatory      2908..2917
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     promoter        complement(2969..3172)
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     complement(2998..3018)
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     enhancer        3173..3476
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     rep_origin      complement(3651..4239)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(3731..3750)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     polyA_signal    4568..4615
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase 
                     polyadenylation signal (Cole and Stacy, 1985)"
     primer_bind     4640..4659
                     /label=TK-pA-R
                     /note="Thymidine kinase polyA, reverse primer"
     CDS             complement(4847..5641)
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     CDS             complement(4847..5641)
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(4958..4977)
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     primer_bind     5568..5587
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     promoter        complement(join(5676..5706,1..327))
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      join(5690..5706,1..119)
                     /label=SV40 ori
                     /note="SV40 origin of replication"
ORIGIN
        1 cctcactact tctggaatag ctcagaggcc gaggcggcct cggcctctgc ataaataaaa
       61 aaaattagtc agccatgggg cggagaatgg gcggaactgg gcggagttag gggcgggatg
      121 ggcggagtta ggggcgggac tatggttgct gactaattga gatgcatgct ttgcatactt
      181 ctgcctgctg gggagcctgg ggactttcca cacctggttg ctgactaatt gagatgcatg
      241 ctttgcatac ttctgcctgc tggggagcct ggggactttc cacaccctaa ctgacacaca
      301 ttccacagct ggttctttcc gcctcaggac tcttcctttt tcaatattat tgaagcattt
      361 atcagggtta ttgtctcatg agcggataca tatttgaatg tatttagaaa aataaacaaa
      421 taggggttcc gcgcacattt ccccgaaaag tgccacctga cgcgccctgt agcggcgcat
      481 taagcgcggc gggtgtggtg gttacgcgca gcgtgaccgc tacacttgcc agcgccctag
      541 cgcccgctcc tttcgctttc ttcccttcct ttctcgccac gttcgccggc tttccccgtc
      601 aagctctaaa tcgggggctc cctttagggt tccgatttag tgctttacgg cacctcgacc
      661 ccaaaaaact tgattagggt gatggttcac gtagtgggcc atcgccctga tagacggttt
      721 ttcgcccttt gacgttggag tccacgttct ttaatagtgg actcttgttc caaactggaa
      781 caacactcaa ccctatctcg gtctattctt ttgatttata agggattttg ccgatttcgg
      841 cctattggtt aaaaaatgag ctgatttaac aaaaatttaa cgcgaatttt aacaaaatat
      901 taacgcttac aatttacgcc ttaagataca ttgatgagtt tggacaaacc acaactagaa
      961 tgcagtgaaa aaaatgcttt atttgtgaaa tttgtgatgc tattgcttta tttgtaacca
     1021 ttataagctg caataaacaa gttaacaaca acaattgcat tcattttatg tttcaggttc
     1081 agggggaggt gtgggaggtt ttttaaagca agtaaaacct ctacaaatgt ggtatggctg
     1141 attatgatct agagtcgcgg ccgccactgt gctggatatc tgcagaattc agctagctct
     1201 gctggatggc gatgcccatc accacggctt gctgtttcag gaactcgatt tcccaggtct
     1261 gccagctccg aggggcagcc agttggtggg ccaccagcag gccccacagc ttcttgccct
     1321 tgatgatggg gacagccaga atggcccgca cctgaaactg gcccagcagg tctctgtagc
     1381 tctccagcag attggcctgg tagatgtcgg cgatggcctg gtatctgccg tgcaagtatt
     1441 cctcacctct ggtttccatg aagtagcagt cccgcacctg ggtcttcagg atgctgttcc
     1501 atctgtcgtc cacggcttcc acgacgacgc tgccggagta atcttcctcg aactggaaca
     1561 cgcacactct gtccacttgc aggaactggc gcacctcggt cacgatggtg ttcagcatct
     1621 tgtccaggtt tgccattacc ggtggatccg cgccggtgga gccggtggag ccggtggagc
     1681 cgaattccga gatggtctga tagcccgcgt gactcctctt cctgccgatg aggtaggcga
     1741 tgaggacgat gaggaccagc cctgccaggg ccccgcccac agcaatgggg atcagcatgt
     1801 tgttaccgtc ctgtacacac tcttccacag acccaaacct gtcactttct accctgaaag
     1861 cctggacttg cacgctgaag acattgaggg cgagcgcctt gctgacaaag atgtgctcct
     1921 cactgttgca cttgtatgag ttgccgacac tggcctgaag agctttcagg gaatagttgg
     1981 aggtgctgaa cgtgggctct atggcatcag gaagagtcat gttcaactga actccttgca
     2041 ggaaaaacag gctagaagtg gcattcatcc caaactgcag ctccaggact ctgctcttgt
     2101 tccccacctt cagggtcacc aactgggcac cgcaagtccc actatatttg tcacttgggt
     2161 tgatgttgaa tgctctggtc acagtcgtgt tgtccttctt catgtaggtg atgttgagtt
     2221 gcagtgccat agaggccagc aggcaggttc cattgtcacc agtcacattg tacttggaca
     2281 cactggggtt tgtgggcaca agtggtggtg aggggctggg tggcccagta gttggggaag
     2341 gttgatcctg tgggcagcgt gtctcttcct tgctgaagtt gctactcggc aggtaggcct
     2401 ggatagtagc gtcccagagc acaatggtca cattcttcat gtagaccctg atgtcgctga
     2461 cacatcggta tgttttgttg atgtctgcct tgatgtcagt tgtggaatcc acagtgtcgg
     2521 gccctttgga gctggcattg ggaaagaatt gtgtgtctga caggttatat gtgaaataca
     2581 tgtgctggac actgtaacgt gttgtgtttt ttgtgaaggt gagtttcagt aaatatcctt
     2641 ctccaaaggt gattgcgagg gtgggctcag aagcattctt ttcaccacaa gagctgctat
     2701 tcttcaggac ttctgcagag gctggcaggg tcatattcga gaccttagaa acatgtccag
     2761 cctcataggt ggtcagaaag gaggcagaga agctggccat tatacacgct gtgccgttgt
     2821 tgtctttcac ctcgaacagt gctggggcgc tgtgtgcaag gcctgccagc agcaacagga
     2881 gcagcggacg ccgggcgccc ggggccgcca tggtggcgct cgagatctga gtccggtagc
     2941 gctagcggat ctgacggttc actaaaccag ctctgcttat atagacctcc caccgtacac
     3001 gcctaccgcc catttgcgtc aatggggcgg agttgttacg acattttgga aagtcccgtt
     3061 gattttggtg ccaaaacaaa ctcccattga cgtcaatggg gtggagactt ggaaatcccc
     3121 gtgagtcaaa ccgctatcca cgcccattga tgtactgcca aaaccgcatc accatggtaa
     3181 tagcgatgac taatacgtag atgtactgcc aagtaggaaa gtcccataag gtcatgtact
     3241 gggcataatg ccaggcgggc catttaccgt cattgacgtc aatagggggc gtacttggca
     3301 tatgatacac ttgatgtact gccaagtggg cagtttaccg taaatactcc acccattgac
     3361 gtcaatggaa agtccctatt ggcgttacta tgggaacata cgtcattatt gacgtcaatg
     3421 ggcgggggtc gttgggcggt cagccaggcg ggccatttac cgtaagttat gtaacgcgga
     3481 actccatata tgggctatga actaatgacc ccgtaattga ttactattaa taactaatgc
     3541 atggcggtaa tacggttatc cacagaatca ggggataacg caggaaagaa catgtgagca
     3601 aaaggccagc aaaaggccag gaaccgtaaa aaggccgcgt tgctggcgtt tttccatagg
     3661 ctccgccccc ctgacgagca tcacaaaaat cgacgctcaa gtcagaggtg gcgaaacccg
     3721 acaggactat aaagatacca ggcgtttccc cctggaagct ccctcgtgcg ctctcctgtt
     3781 ccgaccctgc cgcttaccgg atacctgtcc gcctttctcc cttcgggaag cgtggcgctt
     3841 tctcatagct cacgctgtag gtatctcagt tcggtgtagg tcgttcgctc caagctgggc
     3901 tgtgtgcacg aaccccccgt tcagcccgac cgctgcgcct tatccggtaa ctatcgtctt
     3961 gagtccaacc cggtaagaca cgacttatcg ccactggcag cagccactgg taacaggatt
     4021 agcagagcga ggtatgtagg cggtgctaca gagttcttga agtggtggcc taactacggc
     4081 tacactagaa gaacagtatt tggtatctgc gctctgctga agccagttac cttcggaaaa
     4141 agagttggta gctcttgatc cggcaaacaa accaccgctg gtagcggtgg tttttttgtt
     4201 tgcaagcagc agattacgcg cagaaaaaaa ggatctcaag aagatccttt gatcttttct
     4261 acggggtctg acgctcagtg gaacgaaaac tcacgttaag ggattttggt catgagatta
     4321 tcaaaaagga tcttcaccta gatcctttta aattaaaaat gaagttttaa atcaatctaa
     4381 agtatatatg agtaacctga ggctatggca gggcctgccg ccccgacgtt ggctgcgagc
     4441 cctgggcctt cacccgaact tggggggtgg ggtggggaaa aggaagaaac gcgggcgtat
     4501 tggccccaat ggggtctcgg tggggtatcg acagagtgcc agccctggga ccgaaccccg
     4561 cgtttatgaa caaacgaccc aacaccgtgc gttttattct gtctttttat tgccgtcata
     4621 gcgcgggttc cttccggtat tgtctccttc cgtgtttcag ttagcctccc cctagggtgg
     4681 gcgaagaact ccagcatgag atccccgcgc tggaggatca tccagccggc gtcccggaaa
     4741 acgattccga agcccaacct ttcatagaag gcggcggtgg aatcgaaatc tcgtgatggc
     4801 aggttgggcg tcgcttggtc ggtcatttcg aaccccagag tcccgctcag aagaactcgt
     4861 caagaaggcg atagaaggcg atgcgctgcg aatcgggagc ggcgataccg taaagcacga
     4921 ggaagcggtc agcccattcg ccgccaagct cttcagcaat atcacgggta gccaacgcta
     4981 tgtcctgata gcggtccgcc acacccagcc ggccacagtc gatgaatcca gaaaagcggc
     5041 cattttccac catgatattc ggcaagcagg catcgccatg ggtcacgacg agatcctcgc
     5101 cgtcgggcat gctcgccttg agcctggcga acagttcggc tggcgcgagc ccctgatgct
     5161 cttcgtccag atcatcctga tcgacaagac cggcttccat ccgagtacgt gctcgctcga
     5221 tgcgatgttt cgcttggtgg tcgaatgggc aggtagccgg atcaagcgta tgcagccgcc
     5281 gcattgcatc agccatgatg gatactttct cggcaggagc aaggtgagat gacaggagat
     5341 cctgccccgg cacttcgccc aatagcagcc agtcccttcc cgcttcagtg acaacgtcga
     5401 gcacagctgc gcaaggaacg cccgtcgtgg ccagccacga tagccgcgct gcctcgtctt
     5461 gcagttcatt cagggcaccg gacaggtcgg tcttgacaaa aagaaccggg cgcccctgcg
     5521 ctgacagccg gaacacggcg gcatcagagc agccgattgt ctgttgtgcc cagtcatagc
     5581 cgaatagcct ctccacccaa gcggccggag aacctgcgtg caatccatct tgttcaatca
     5641 tgcgaaacga tcctcatcct gtctcttgat cgatctttgc aaaagcctag gcctccaaaa
     5701 aagcct
//
