LOCUS       sequence-410780-       13860 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  HYG(1-89)-NpuDnaE(N)_GFPuv.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 13860)
  AUTHORS   Yuan G, Lu H, De K, Hassan MM, Liu Y, Islam MT, Muchero W, Tuskan 
            GA, Yang X
  TITLE     Split selectable marker systems utilizing inteins facilitate gene 
            stacking in plants.
  JOURNAL   Commun Biol. 2023 May 26;6(1):567. doi: 10.1038/s42003-023-04950-8.
  PUBMED    37237044
REFERENCE   2  (bases 1 to 13860)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Commun 
            Biol."; date: "2023-05-26"; volume: "6(1):567. doi"; pages: " 
            10.1038/s42003-023-04950-8"
COMMENT     Sequence Label: pAXY0014
FEATURES             Location/Qualifiers
     source          1..13860
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        complement(16..361)
                     /label=CaMV 35S promoter
                     /note="strong constitutive promoter from cauliflower mosaic
                     virus"
     primer_bind     723..745
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     737..754
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     738..754
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        1269..1614
                     /label=CaMV 35S promoter
                     /note="strong constitutive promoter from cauliflower mosaic
                     virus"
     terminator      2400..2648
                     /label=HSP terminator
                     /note="efficient transcription terminator from the 
                     Arabidopsis thaliana heat shock protein 18.2 gene (Nagaya 
                     et al., 2010)"
     primer_bind     complement(3337..3353)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(3337..3353)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(3350..3372)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    3361..3377
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(3385..3391,3392..3409,3410..3415))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    3430..3451
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        3642..4318
                     /label=CaMV 35S promoter (enhanced)
                     /note="cauliflower mosaic virus 35S promoter with a 
                     duplicated enhancer region"
     polyA_signal    4951..5125
                     /label=CaMV poly(A) signal
                     /note="cauliflower mosaic virus polyadenylation signal"
     misc_feature    5203..5227
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     CDS             5652..6446
                     /codon_start=1
                     /gene="aphA-3"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin"
                     /translation="MAKMRISPELKKLIEKYRCVKDTEGMSPAKVYKLVGENENLYLKM
                     TDSRYKGTTYDVEREKDMMLWLEGKLPVPKVLHFERHDGWSNLLMSEADGVLCSEEYED
                     EQSPEKIIELYAECIRLFHSIDISDCPYTNSLDSRLAELDYLLNNDLADVDCENWEEDT
                     PFKDPRELYDFLKTEKPEEELVFSHGDLGDSNIFVKDGKVSGFIDLGRSGRADKWYDIA
                     FCVRSIREDIGEEQYVELFFDLLGIKPDWEKIKYYILLDELF"
     rep_origin      6533..7121
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     7022..7041
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     7275..7292
                     /label=L4440
                     /note="L4440 vector, forward primer"
     misc_feature    7307..7447
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     complement(7373..7392)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     7533..7555
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     rep_origin      7791..7985
                     /label=pVS1 oriV
                     /note="origin of replication for the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
     CDS             complement(8051..9124)
                     /codon_start=1
                     /product="replication protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 RepA
                     /translation="MSGRKPSGPVQIGAALGDDLVEKLKAAQAAQRQRIEAEARPGESW
                     QAAADRIRKESRQPPAAGAPSIRKPPKGDEQPDFFVPMLYDVGTRDSRSIMDVAVFRLS
                     KRDRRAGEVIRYELPDGHVEVSAGPAGMASVWDYDLVLMAVSHLTESMNRYREGKGDKP
                     GRVFRPHVADVLKFCRRADGGKQKDDLVETCIRLNTTHVAMQRTKKAKNGRLVTVSEGE
                     ALISRYKIVKSETGRPEYIEIELADWMYREITEGKNPDVLTVHPDYFLIDPGIGRFLYR
                     LARRAAGKAEARWLFKTIYERSGSAGEFKKFCFTVRKLIGSNDLPEYDLKEEAGQAGPI
                     LVMRYRNLIEGEASAGS"
     CDS             complement(9553..10182)
                     /codon_start=1
                     /product="stability protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 StaA
                     /translation="MKVIAVLNQKGGSGKTTIATHLARALQLAGADVLLVDSDPQGSAR
                     DWAAVREDQPLTVVGIDRPTIDRDVKAIGRRDFVVIDGAPQAADLAVSAIKAADFVLIP
                     VQPSPYDIWATADLVELVKQRIEVTDGRLQAAFVVSRAIKGTRIGGEVAEALAGYELPI
                     LESRITQRVSYPGTAAAGTTVLESEPEGDAAREVQALAAEIKSKLI"
     misc_feature    11483..11507
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     terminator      complement(11529..11781)
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     CDS             complement(11813..11830)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             complement(join(11837..13654,13845..13859))
                     /codon_start=1
                     /product="beta-glucuronidase"
                     /label=GUSPlus(TM)
                     /note="codon-optimized Staphylococcus gusA gene with a 
                     catalase intron to ensure expression in plants but not 
                     bacteria"
                     /translation="MVDLRNRRTSLYPINTETRGVFDLNGVWNFKLDYGKGLEEKWYES
                     KLTDTISMAVPSSYNDIGVTKEIRNHIGYVWYEREFTVPAYLKDQRIVLRFGSATHKAI
                     VYVNGELVVEHKGGFLPFEAEINNSLRDGMNRVTVAVDNILDDSTLPVGLYSERHEEGL
                     GKVIRNKPNFDFFNYAGLHRPVKIYTTPFTYVEDISVVTDFNGPTGTVTYTVDFQGKAE
                     TVKVSVVDEEGKVVASTEGLSGNVEIPNVILWEPLNTYLYQIKVELVNDGLTIDVYEEP
                     FGVRTVEVNDGKFLINNKPFYFKGFGKHEDTPINGRGFNEASNVMDFNILKWIGANSFR
                     TAHYPYSEELMRLADREGLVVIDETPAVGVHLNFMATTGLGEGSERVSTWEKIRTFEHH
                     QDVLRELVSRDKNHPSVVMWSIANEAATEEEGAYEYFKPLVELTKELDPQKRPVTIVLF
                     VMATPETDKVAELIDVIALNRYNGWYFDGGDLEAAKVHLRQEFHAWNKRCPGKPIMITE
                     YGADTVAGFHDIDPVMFTEEYQVEYYQANHVVFDEFENFVGEQAWNFADFATSQGVMRV
                     QGNKKGVFTRDRKPKLAAHVFRERWTNIPDFGYKN"
     intron          13655..13844
                     /label=cat1 intron
                     /note="castor bean catalase intron, modified"
ORIGIN
        1 gtcaagagtc ccccgtgttc tctccaaatg aaatgaactt ccttatatag aggaagggtc
       61 ttgcgaagga tagtgggatt gtgcgtcatc ccttacgtca gtggagatat cacatcaatc
      121 cacttgcttt gaagacgtgg ttggaacgtc ttctttttcc acgatgctcc tcgtgggtgg
      181 gggtccatct ttgggaccac tgtcggcaga ggcatcttca acgatggcct ttcctttatc
      241 gcaatgatgg catttgtagg agccaccttc cttttccact atcttcacaa taaagtgaca
      301 gatagctggg caatggaatc cgaggaggtt tccggatatt accctttgtt gaaaagtctc
      361 aattgccctt tggtcttctg agactgtatc tttgatattt ttggagtaga caagtgtgtc
      421 gtgctccacc atgttgacga agattttctt cttgtcattg agtcgtaaga gactctgtat
      481 gaactgttcg ccagtcttta cggcgagttc tgttaggtcc tctatttgaa tctttgactc
      541 catgaagcta aactgaaggc gggaaacgac aatctgatcc aagctcaagc tgctctagca
      601 ttcgccattc aggctgcgca actgttggga agggcgatcg gtgcgggcct cttcgctatt
      661 acgccagctg gcgaaagggg gatgtgctgc aaggcgatta agttgggtaa cgccagggtt
      721 ttcccagtca cgacgttgta aaacgacggc cagtgccaag cttgcatgcc tgcaggtccc
      781 cagattagcc ttttcaattt cagaaagaat gctaacccac agatggttag agaggcttac
      841 gcagcaggtc tcatcaagac gatctacccg agcaataatc tccaggaaat caaatacctt
      901 cccaagaagg ttaaagatgc agtcaaaaga ttcaggacta actgcatcaa gaacacagag
      961 aaagatatat ttctcaagat cagaagtact attccagtat ggacgattca aggcttgctt
     1021 cacaaaccaa ggcaagtaat agagattgga gtctctaaaa aggtagttcc cactgaatca
     1081 aaggccatgg agtcaaagat tcaaatagag gacctaacag aactcgccgt aaagactggc
     1141 gaacagttca tacagagtct cttacgactc aatgacaaga agaaaatctt cgtcaacatg
     1201 gtggagcacg acacacttgt ctactccaaa aatatcaaag atacagtctc agaagaccaa
     1261 agggcaattg agacttttca acaaagggta atatccggaa acctcctcgg attccattgc
     1321 ccagctatct gtcactttat tgtgaagata gtggaaaagg aaggtggctc ctacaaatgc
     1381 catcattgcg ataaaggaaa ggccatcgtt gaagatgcct ctgccgacag tggtcccaaa
     1441 gatggacccc cacccacgag gagcatcgtg gaaaaagaag acgttccaac cacgtcttca
     1501 aagcaagtgg attgatgtga tatctccact gacgtaaggg atgacgcaca atcccactat
     1561 ccttcgcaag acccttcctc tatataagga agttcatttc atttggagag aacacggggg
     1621 actcaaacat tactcattca caaaaccatc ttaaagcaac tacacaagtc ttgaaatttt
     1681 ctcatatttt ctatttacta tataaacttt taatcaaatc aagattaaag ttaattaaat
     1741 gacaaccttc aaaatcgagt cccggatcca cggcaacctc aacggggaga agttcgagtt
     1801 ggttggaggt ggagtaggtg aggagggtcg cctcgagatt gagatgaaga ctaaagataa
     1861 accactggca ttctctccct tcctgctgac cacttgcatg ggttacgggt tctaccactt
     1921 cgccagcttc ccaaagggga ttaagaacat ctatcttcat gctgcaacga acggaggtta
     1981 caccaacacc aggaaggaga tctatgaaga cggcggcatc ttggaggtca acttccgtta
     2041 cacttacgag ttcaacaaga tcatcggtga cgtcgagtgc attggacatg gattcccaag
     2101 tcagagtccg atcttcaagg acacgatcgt gaagagttgt cccacggtgg acctgatgtt
     2161 gccaatgtcc gggaacatca tcgccagctc ctacgcttac gccttccaac tgaaggacgg
     2221 ctctttctac acggcagaag tcaagaacaa catagacttc aagaatccaa tccacgagtc
     2281 cttctcgaag tcagggccca tgttcaccca cagacgtgtc gaggagactc tcaccaagga
     2341 gaaccttgcc atagtggagt accagcaggt tttcaacagc gccccaagag acatgtagat
     2401 atgaagatga agatgaaata tttggtgtgt caaataaaaa gcttgtgtgc ttaagtttgt
     2461 gtttttttct tggcttgttg tgttatgaat ttgtggcttt ttctaatatt aaatgaatgt
     2521 aagatctcat tataatgaat aaacaaatgt ttctataatc cattgtgaat gttttgttgg
     2581 atctcttctg cagcatataa ctactgtatg tgctatggta tggactatgg aatatgatta
     2641 aagataagat gggctcatag agtaaaacga ggcgagggac ctataaacct cccttcatca
     2701 tgctatttca tgatctattt tataaaataa agatgtagaa aaaagtaagc gtaataaccg
     2761 caaaacaaat gatttaaaac atggcacata atgaggagat taagttcggt ttacgtttat
     2821 tttagtacta attgtaacgt gagactacgt atcgggaatc gcctaattaa agcattaatg
     2881 cgaacctgat tagattcacc gaccctccta tcgtgtcgac ctttctgttt cttagaattt
     2941 tttggtagtc tatgtactaa taatgtcagc ttcgtattta tttcataagc aatttgcatt
     3001 tgcaatttgt tttttacttt tatttttatt gtattgtgga atgtggactc gtaccaacat
     3061 gaagttatat accaccaaaa aaattacagt tagtcaaaag attcacgagt gagagctact
     3121 tatgattgtc ttttacgtat atgtctaatt gtctatttgc tcaataatct ttgtactttc
     3181 ttttgtcgtt gataaaatca caaagttcca aaagtaatcg aatgatttgc ttttaagaaa
     3241 agaagagctc aataattcaa catatatctg tacacaatta ccgcctttga gtgagctcta
     3301 gatgccacct gacgtctaag aagaattcgt aatcatgtca tagctgtttc ctgtgtgaaa
     3361 ttgttatccg ctcacaattc cacacaacat acgagccgga agcataaagt gtaaagcctg
     3421 gggtgcctaa tgagtgagct aactcacatt aattgcgttg cgctcactgc ccgctttcca
     3481 gtcgggaaac ctgtcgtgcc agctgcatta atgaatcggc caacgcgcgg ggagaggcgg
     3541 tttgcgtatt ggctagagca gcttgccaac atggtggagc acgacactct cgtctactcc
     3601 aagaatatca aagatacagt ctcagaagac caaagggcta ttgagacttt tcaacaaagg
     3661 gtaatatcgg gaaacctcct cggattccat tgcccagcta tctgtcactt catcaaaagg
     3721 acagtagaaa aggaaggtgg cacctacaaa tgccatcatt gcgataaagg aaaggctatc
     3781 gttcaagatg cctctgccga cagtggtccc aaagatggac ccccacccac gaggagcatc
     3841 gtggaaaaag aagacgttcc aaccacgtct tcaaagcaag tggattgatg tgaacatggt
     3901 ggagcacgac actctcgtct actccaagaa tatcaaagat acagtctcag aagaccaaag
     3961 ggctattgag acttttcaac aaagggtaat atcgggaaac ctcctcggat tccattgccc
     4021 agctatctgt cacttcatca aaaggacagt agaaaaggaa ggtggcacct acaaatgcca
     4081 tcattgcgat aaaggaaagg ctatcgttca agatgcctct gccgacagtg gtcccaaaga
     4141 tggaccccca cccacgagga gcatcgtgga aaaagaagac gttccaacca cgtcttcaaa
     4201 gcaagtggat tgatgtgata tctccactga cgtaagggat gacgcacaat cccactatcc
     4261 ttcgcaagac ccttcctcta tataaggaag ttcatttcat ttggagagga cacgctgaaa
     4321 tcaccagtct ctctctacaa atctatctct ctcgagcttt cgcagatatg aaaaagcctg
     4381 aactcaccgc gacgtctgtc gagaagtttc tgatcgaaaa gttcgacagc gtctccgacc
     4441 tgatgcagct ctcggagggc gaagaatctc gtgctttcag cttcgatgta ggagggcgtg
     4501 gatatgtcct gcgggtaaat agctgcgccg atggtttcta caaagatcgt tatgtttatc
     4561 ggcactttgc atcggccgcg ctcccgattc cggaagtgct tgacattggg gagtttagcg
     4621 agagcctgac ctattgcttg tcctacgaga ccgagattct tactgttgag tatggattgc
     4681 ttccaatcgg caagatcgta gagaaacgta ttgagtgtac agtttattct gttgacaaca
     4741 acggaaatat ctatactcag cctgttgctc aatggcacga tagaggggaa caagaggttt
     4801 tcgagtattg ccttgaggat ggtagcctca ttcgagcaac aaaagatcac aaatttatga
     4861 cagtggatgg tcagatgtta cctatcgatg aaattttcga aagggaatta gacctcatga
     4921 gagtggataa cctccctaat taggctcgag tttctccata ataatgtgtg agtagttccc
     4981 agataaggga attagggttc ctatagggtt tcgctcatgt gttgagcata taagaaaccc
     5041 ttagtatgta tttgtatttg taaaatactt ctatcaataa aatttctaat tcctaaaacc
     5101 aaaatccagt actaaaatcc agatcccccg aattaattcg gcgttaattc agtacattaa
     5161 aaacgtccgc aatgtgttat taagttgtct aagcgtcaat ttgtttacac cacaatatat
     5221 cctgccacca gccagccaac agctccccga ccggcagctc ggcacaaaat caccactcga
     5281 tacaggcagc ccatcagtcc gggacggcgt cagcgggaga gccgttgtaa ggcggcagac
     5341 tttgctcatg ttaccgatgc tattcggaag aacggcaact aagctgccgg gtttgaaaca
     5401 cggatgatct cgcggagggt agcatgttga ttgtaacgat gacagagcgt tgctgcctgt
     5461 gatcaccgcg gtttcaaaat cggctccgtc gatactatgt tatacgccaa ctttgaaaac
     5521 aactttgaaa aagctgtttt ctggtattta aggttttaga atgcaaggaa cagtgaattg
     5581 gagttcgtct tgttataatt agcttcttgg ggtatcttta aatactgtag aaaagaggaa
     5641 ggaaataata aatggctaaa atgagaatat caccggaatt gaaaaaactg atcgaaaaat
     5701 accgctgcgt aaaagatacg gaaggaatgt ctcctgctaa ggtatataag ctggtgggag
     5761 aaaatgaaaa cctatattta aaaatgacgg acagccggta taaagggacc acctatgatg
     5821 tggaacggga aaaggacatg atgctatggc tggaaggaaa gctgcctgtt ccaaaggtcc
     5881 tgcactttga acggcatgat ggctggagca atctgctcat gagtgaggcc gatggcgtcc
     5941 tttgctcgga agagtatgaa gatgaacaaa gccctgaaaa gattatcgag ctgtatgcgg
     6001 agtgcatcag gctctttcac tccatcgaca tatcggattg tccctatacg aatagcttag
     6061 acagccgctt agccgaattg gattacttac tgaataacga tctggccgat gtggattgcg
     6121 aaaactggga agaagacact ccatttaaag atccgcgcga gctgtatgat tttttaaaga
     6181 cggaaaagcc cgaagaggaa cttgtctttt cccacggcga cctgggagac agcaacatct
     6241 ttgtgaaaga tggcaaagta agtggcttta ttgatcttgg gagaagcggc agggcggaca
     6301 agtggtatga cattgccttc tgcgtccggt cgatcaggga ggatatcggg gaagaacagt
     6361 atgtcgagct attttttgac ttactgggga tcaagcctga ttgggagaaa ataaaatatt
     6421 atattttact ggatgaattg ttttagtacc tagaatgcat gaccaaaatc ccttaacgtg
     6481 agttttcgtt ccactgagcg tcagaccccg tagaaaagat caaaggatct tcttgagatc
     6541 ctttttttct gcgcgtaatc tgctgcttgc aaacaaaaaa accaccgcta ccagcggtgg
     6601 tttgtttgcc ggatcaagag ctaccaactc tttttccgaa ggtaactggc ttcagcagag
     6661 cgcagatacc aaatactgtc cttctagtgt agccgtagtt aggccaccac ttcaagaact
     6721 ctgtagcacc gcctacatac ctcgctctgc taatcctgtt accagtggct gctgccagtg
     6781 gcgataagtc gtgtcttacc gggttggact caagacgata gttaccggat aaggcgcagc
     6841 ggtcgggctg aacggggggt tcgtgcacac agcccagctt ggagcgaacg acctacaccg
     6901 aactgagata cctacagcgt gagctatgag aaagcgccac gcttcccgaa gggagaaagg
     6961 cggacaggta tccggtaagc ggcagggtcg gaacaggaga gcgcacgagg gagcttccag
     7021 ggggaaacgc ctggtatctt tatagtcctg tcgggtttcg ccacctctga cttgagcgtc
     7081 gatttttgtg atgctcgtca ggggggcgga gcctatggaa aaacgccagc aacgcggcct
     7141 ttttacggtt cctggccttt tgctggcctt ttgctcacat gttctttcct gcgttatccc
     7201 ctgattctgt ggataaccgt attaccgcct ttgagtgagc tgataccgct cgccgcagcc
     7261 gaacgaccga gcgcagcgag tcagtgagcg aggaagcgga agagcgcctg atgcggtatt
     7321 ttctccttac gcatctgtgc ggtatttcac accgcatatg gtgcactctc agtacaatct
     7381 gctctgatgc cgcatagtta agccagtata cactccgcta tcgctacgtg actgggtcat
     7441 ggctgcgccc cgacacccgc caacacccgc tgacgcgccc tgacgggctt gtctgctccc
     7501 ggcatccgct tacagacaag ctgtgaccgt ctccgggagc tgcatgtgtc agaggttttc
     7561 accgtcatca ccgaaacgcg cgaggcaggg tgccttgatg tgggcgccgg cggtcgagtg
     7621 gcgacggcgc ggcttgtccg cgccctggta gattgcctgg ccgtaggcca gccatttttg
     7681 agcggccagc ggccgcgata ggccgacgcg aagcggcggg gcgtagggag cgcagcgacc
     7741 gaagggtagg cgctttttgc agctcttcgg ctgtgcgctg gccagacagt tatgcacagg
     7801 ccaggcgggt tttaagagtt ttaataagtt ttaaagagtt ttaggcggaa aaatcgcctt
     7861 ttttctcttt tatatcagtc acttacatgt gtgaccggtt cccaatgtac ggctttgggt
     7921 tcccaatgta cgggttccgg ttcccaatgt acggctttgg gttcccaatg tacgtgctat
     7981 ccacaggaaa gagacctttt cgaccttttt cccctgctag ggcaatttgc cctagcatct
     8041 gctccgtaca ttaggaaccg gcggatgctt cgccctcgat caggttgcgg tagcgcatga
     8101 ctaggatcgg gccagcctgc cccgcctcct ccttcaaatc gtactccggc aggtcatttg
     8161 acccgatcag cttgcgcacg gtgaaacaga acttcttgaa ctctccggcg ctgccactgc
     8221 gttcgtagat cgtcttgaac aaccatctgg cttctgcctt gcctgcggcg cggcgtgcca
     8281 ggcggtagag aaaacggccg atgccgggat cgatcaaaaa gtaatcgggg tgaaccgtca
     8341 gcacgtccgg gttcttgcct tctgtgatct cgcggtacat ccaatcagct agctcgatct
     8401 cgatgtactc cggccgcccg gtttcgctct ttacgatctt gtagcggcta atcaaggctt
     8461 caccctcgga taccgtcacc aggcggccgt tcttggcctt cttcgtacgc tgcatggcaa
     8521 cgtgcgtggt gtttaaccga atgcaggttt ctaccaggtc gtctttctgc tttccgccat
     8581 cggctcgccg gcagaacttg agtacgtccg caacgtgtgg acggaacacg cggccgggct
     8641 tgtctccctt cccttcccgg tatcggttca tggattcggt tagatgggaa accgccatca
     8701 gtaccaggtc gtaatcccac acactggcca tgccggccgg ccctgcggaa acctctacgt
     8761 gcccgtctgg aagctcgtag cggatcacct cgccagctcg tcggtcacgc ttcgacagac
     8821 ggaaaacggc cacgtccatg atgctgcgac tatcgcgggt gcccacgtca tagagcatcg
     8881 gaacgaaaaa atctggttgc tcgtcgccct tgggcggctt cctaatcgac ggcgcaccgg
     8941 ctgccggcgg ttgccgggat tctttgcgga ttcgatcagc ggccgcttgc cacgattcac
     9001 cggggcgtgc ttctgcctcg atgcgttgcc gctgggcggc ctgcgcggcc ttcaacttct
     9061 ccaccaggtc atcacccagc gccgcgccga tttgtaccgg gccggatggt ttgcgaccgc
     9121 tcacgccgat tcctcgggct tgggggttcc agtgccattg cagggccggc aggcaaccca
     9181 gccgcttacg cctggccaac cgcccgttcc tccacacatg gggcattcca cggcgtcggt
     9241 gcctggttgt tcttgatttt ccatgccgcc tcctttagcc gctaaaattc atctactcat
     9301 ttattcattt gctcatttac tctggtagct gcgcgatgta ttcagatagc agctcggtaa
     9361 tggtcttgcc ttggcgtacc gcgtacatct tcagcttggt gtgatcctcc gccggcaact
     9421 gaaagttgac ccgcttcatg gctggcgtgt ctgccaggct ggccaacgtt gcagccttgc
     9481 tgctgcgtgc gctcggacgg ccggcactta gcgtgtttgt gcttttgctc attttctctt
     9541 tacctcatta actcaaatga gttttgattt aatttcagcg gccagcgcct ggacctcgcg
     9601 ggcagcgtcg ccctcgggtt ctgattcaag aacggttgtg ccggcggcgg cagtgcctgg
     9661 gtagctcacg cgctgcgtga tacgggactc aagaatgggc agctcgtacc cggccagcgc
     9721 ctcggcaacc tcaccgccga tgcgcgtgcc tttgatcgcc cgcgacacga caaaggccgc
     9781 ttgtagcctt ccatccgtga cctcaatgcg ctgcttaacc agctccacca ggtcggcggt
     9841 ggcccatatg tcgtaagggc ttggctgcac cggaatcagc acgaagtcgg ctgccttgat
     9901 cgcggacaca gccaagtccg ccgcctgggg cgctccgtcg atcactacga agtcgcgccg
     9961 gccgatggcc ttcacgtcgc ggtcaatcgt cgggcggtcg atgccgacaa cggttagcgg
    10021 ttgatcttcc cgcacggccg cccaatcgcg ggcactgccc tggggatcgg aatcgactaa
    10081 cagaacatcg gccccggcga gttgcagggc gcgggctaga tgggttgcga tggtcgtctt
    10141 gcctgacccg cctttctggt taagtacagc gataaccttc atgcgttccc cttgcgtatt
    10201 tgtttattta ctcatcgcat catatacgca gcgaccgcat gacgcaagct gttttactca
    10261 aatacacatc acctttttag acggcggcgc tcggtttctt cagcggccaa gctggccggc
    10321 caggccgcca gcttggcatc agacaaaccg gccaggattt catgcagccg cacggttgag
    10381 acgtgcgcgg gcggctcgaa cacgtacccg gccgcgatca tctccgcctc gatctcttcg
    10441 gtaatgaaaa acggttcgtc ctggccgtcc tggtgcggtt tcatgcttgt tcctcttggc
    10501 gttcattctc ggcggccgcc agggcgtcgg cctcggtcaa tgcgtcctca cggaaggcac
    10561 cgcgccgcct ggcctcggtg ggcgtcactt cctcgctgcg ctcaagtgcg cggtacaggg
    10621 tcgagcgatg cacgccaagc agtgcagccg cctctttcac ggtgcggcct tcctggtcga
    10681 tcagctcgcg ggcgtgcgcg atctgtgccg gggtgagggt agggcggggg ccaaacttca
    10741 cgcctcgggc cttggcggcc tcgcgcccgc tccgggtgcg gtcgatgatt agggaacgct
    10801 cgaactcggc aatgccggcg aacacggtca acaccatgcg gccggccggc gtggtggtgt
    10861 cggcccacgg ctctgccagg ctacgcaggc ccgcgccggc ctcctggatg cgctcggcaa
    10921 tgtccagtag gtcgcgggtg ctgcgggcca ggcggtctag cctggtcact gtcacaacgt
    10981 cgccagggcg taggtggtca agcatcctgg ccagctccgg gcggtcgcgc ctggtgccgg
    11041 tgatcttctc ggaaaacagc ttggtgcagc cggccgcgtg cagttcggcc cgttggttgg
    11101 tcaagtcctg gtcgtcggtg ctgacgcggg catagcccag caggccagcg gcggcgctct
    11161 tgttcatggc gtaatgtctc cggttctagt cgcaagtatt ctactttatg cgactaaaac
    11221 acgcgacaag aaaacgccag gaaaagggca gggcggcagc ctgtcgcgta acttaggact
    11281 tgtgcgacat gtcgttttca gaagacggct gcactgaacg tcagaagccg actgcactat
    11341 agcagcggag gggttggatc aaagtacttt gatcccgagg ggaaccctgt ggttggcatg
    11401 cacatacaaa tggacgaacg gataaacctt ttcacgccct tttaaatatc cgattattct
    11461 aataaacgct cttttctctt aggtttaccc gccaatatat cctgtcaaac actgatagtt
    11521 taattcccga tctagtaaca tagatgacac cgcgcgcgat aatttatcct agtttgcgcg
    11581 ctatattttg ttttctatcg cgtattaaat gtataattgc gggactctaa tcataaaaac
    11641 ccatctcata aataacgtca tgcattacat gttaattatt acatgcttaa cgtaattcaa
    11701 cagaaattat atgataatca tcgcaagacc ggcaacagga ttcaatctta agaaacttta
    11761 ttgccaaatg tttgaacgat cggggaaatt cgagctggtc accaattcac acgtgatggt
    11821 gatggtgatg gctagcgttc ttgtagccga aatctggaat gttggtccag cgctcgcgaa
    11881 agacgtgcgc ggcgagcttc ggcttgcggt cacgagtgaa cacgcccttc ttgtttcctt
    11941 ggacgcgcat cacgccctga gaggtcgcga agtccgcgaa gttccacgct tgctcaccca
    12001 cgaagttctc aaactcatcg aacacgacgt ggttcgcctg gtagtactcg acttgatatt
    12061 cctcggtgaa catcactgga tcaatgtcgt gaaagcccgc aacggtgtct gcgccgtact
    12121 cagtgatcat gatcggcttt cctgggcaac gcttgttcca cgcgtgaaat tcctggcgga
    12181 gatggacttt ggccgcttcg agatcaccgc catcgaagta ccatccgtta tagcgattga
    12241 gcgcgatgac gtcaatcagt tcggcgactt tgtccgtctc cggggtagcc atcacaaaca
    12301 gcacgatcgt gaccggacgc ttctgtgggt cgagttcctt ggtcagctcc accaacggct
    12361 tgaagtactc gtacgcgccc tcttcctcag tcgccgcctc gttggcgatg ctccacatca
    12421 cgacgcttgg atggttcttg tcacgagaca ccagttcacg gagaacgtct tgatggtgct
    12481 caaacgtccg aatcttctcc caggtactga cgcgctcgct gccttcgccg agtcccgtgg
    12541 tggccatgaa gttgaggtgc acgccaactg ccggagtctc gtcgatcacg accagaccct
    12601 cgcgatccgc aagacgcatc aactcttcag agtacggata gtgtgcggtc cggaagctgt
    12661 tggcgccgat ccatttgagg atattgaaat ccatcacatt gctcgcttcg ttaaagccac
    12721 ggccgttgat aggagtgtcc tcatgtttgc caaagccctt gaagtagaac ggtttgttgt
    12781 tgatgaggaa cttgccgtcg ttgacttcca cggtccgcac gccgaacggc tcttcataga
    12841 catcgatggt cagtccgtcg ttcaccagtt ccactttgat ctggtagaga tacgtgttca
    12901 gtggttccca gaggatgaca ttcggaatct ccacgttacc gctcaggccc tcggtgcttg
    12961 cgaccacttt gccttcctca tccacgaccg acactttcac ggtctcggct ttgccttgaa
    13021 agtccaccgt ataggtcaca gtcccggttg ggccattgaa gtcggtcaca accgagatgt
    13081 cctcgacgta cgtaaacggg gtcgtgtaga ttttcaccgg acggtgcagg cctgcatagt
    13141 tgaagaagtc gaagttcggc ttgttacgaa tgacttttcc gaggccctct tcgtggcgct
    13201 cgctgtacag ccccaccggg agggtgctat cgtcgaggat gttgtccacg gcgacggtga
    13261 cgcgattcat gccatcacgc agcgagttgt tgatttccgc ttcgaatggc aggaatccgc
    13321 ccttgtgctc cacgaccagc tcaccattga catagacaat tgctttgtga gttgcagagc
    13381 cgaagcggag cacgatacgc tgatccttca gataggccgg caccgtgaac tcacgttcgt
    13441 accagacata tccgatatgg ttgcggattt ccttggtcac gccaatgtca ttgtaactgc
    13501 ttgggacggc catactaata gtgtcggtca gcttgctttc gtaccacttc tcttccagtc
    13561 ctttcccgta gtccagcttg aagttccaga cgccattgag gtcgaagacg ccacgggtct
    13621 cggtgttgat cgggtacaga ctagttcgtc ggttctgtaa ctatcatcat catcatagac
    13681 acacgaaata aagtaatcag attatcagtt aaagctatgt aatatttaca ccataaccaa
    13741 tcaattaaaa aatagatcag tttaaagaaa gatcaaagct caaaaaaata aaaagagaaa
    13801 agggtcctaa ccaagaaaat gaaggagaaa aactagaaat ttaccctcag atctaccatg
//
