LOCUS       sequence-410784-       11980 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Single-color FUCCI4 senor for transitions of all four cell cylce 
            phases.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11980)
  AUTHORS   Qian Y, Celiker OT, Wang Z, Guner-Ataman B, Boyden ES
  TITLE     Temporally multiplexed imaging of dynamic signaling networks in 
            living cells.
  JOURNAL   Cell. 2023 Dec 7;186(25):5656-5672.e21. doi: 
            10.1016/j.cell.2023.11.010. Epub 2023 Nov 28.
  PUBMED    38029746
REFERENCE   2  (bases 1 to 11980)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2023.11.010"; journalName: "Cell"; date: "2023-12-7-
            7"; volume: "186"; issue: "25"; pages: "5656-5672.e21"
COMMENT     Sequence Label: 
            pLL3.7m-rsGreenF-E-Geminin(1-110)-2A-Dronpa-Cdt-IRES-H1-Skylan62A-2A
            -YFP-SLBP
FEATURES             Location/Qualifiers
     source          1..11980
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        63..366
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        368..566
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     LTR             584..764
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    811..936
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1433..1666
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1851..1895
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    2193..2310
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        2369..2681
                     /label=U6 promoter
                     /note="RNA polymerase III promoter for mouse U6 snRNA (Das 
                     et al., 1988)"
     primer_bind     2600..2620
                     /label=mU6-F
                     /note="Mouse U6 promoter, forward primer"
     primer_bind     2698..2714
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    complement(2756..2789)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     enhancer        2905..3208
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        3209..3412
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     CDS             3444..4160
                     /codon_start=1
                     /product="reversibly switchable enhanced green fluorescent 
                     protein (Grotjohann et al., 2011)"
                     /label=rsEGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVRGEGEGDATNGKLTM
                     KFICTTGKLPVPWPTLVTTLTYGVLCFARYPDHMKQHDFFKSAMPEGYVQERTISFEDD
                     GYYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNLNSHNAYITADKQKNGIR
                     SNFKIRLNVEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQNKLSKDPNEKRDHMVLL
                     EFVTASGITLGMDELYK"
     CDS             4167..4505
                     /codon_start=1
                     /product="GFP-binding fragment of a single-chain camelid 
                     antibody (Rothbauer et al., 2008)"
                     /label=GFP nanobody
                     /translation="VQLVESGGALVQPGGSLRLSCAASGFPVNRYSMRWYRQAPGKERE
                     WVAGMSSAGDRSSYEDSVKGRFTISRDDARNTVYLQMNSLKPEDTAVYYCNVNVGFEYW
                     GQGTQVTVS"
     CDS             4536..4865
                     /codon_start=1
                     /product="degron consisting of residues 1-110 of human 
                     Geminin (Zielke and Edgar, 2015)"
                     /label=Gem1 (1-110)
                     /translation="MNPSMKQKQEEIKENIKNSSVPRRTLKMIQPSASGSLVGRENELS
                     AGLSKRKHRNDHLTSTTSSPGVIVPESSENKNLGGVTQESFDLMIKENPSSQYWKEVAE
                     KRRKAL"
     CDS             4875..4931
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
     CDS             4947..5621
                     /codon_start=1
                     /product="monomeric photoswitchable Dronpa-Green1 
                     fluorescent protein"
                     /label=Dronpa-Green1
                     /translation="MVSVIKPDMKIKLRMEGAVNGHPFAIEGVGLGKPFEGKQSMDLKV
                     KEGGPLPFAYDILTTVFCYGNRVFAKYPENIVDYFKQSFPEGYSWERSMNYEDGGICNA
                     TNDITLDGDCYIYEIRFDGVNFPANGPVMQKRTVKWEPSTEKLYVRDGVLKGDVNMALS
                     LEGGGHYRCDFKTTYKAKKVVQLPDYHFVDHHIEIKSHDKDYSNVNLHEHAEAHSELPR
                     QAK"
     CDS             5676..5948
                     /codon_start=1
                     /product="degron consisting of residues 30-120 of human 
                     Cdt1 (Zielke and Edgar, 2015)"
                     /label=Cdt1 (30-120)
                     /translation="PSPARPALRAPASATSGSRKRARPPAAPGRDQARPPARRRLRLSV
                     DEVSSPSTPEAPDIPACPSPGQKIKKSTPAAGQPPHLTSAQDQDTI"
     misc_feature    5972..6544
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(6139..6156)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     6366..6385
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     CDS             7171..7848
                     /codon_start=1
                     /product="truly monomeric reversibly photoswitchable 
                     fluorescent protein, suitable for nonlinear structured 
                     illumination microscopy (Zhang et al., 2016)"
                     /label=Skylan-NS
                     /note="mEos3.1 derivative that eliminates the green-to-red 
                     conversion"
                     /translation="MSAIKPDMKIKLRMEGNVNGHHFVIDGDGTGKPFEGKQSMDLEVK
                     EGGPLPFAFDILTTAFAYGNRVFAKYPDNIQDYFKQSFPKGYSWERSLTFEDGGICNAR
                     NDITMEGDTFYNKVRFYGTNFPANGPVMQKKTLKWEPSTEKMYVRDGVLTGDVEMALLL
                     EGNAHYRCDFRTTYKAKEKGVKLPGAHFVDHCIEILSHDKDYNKVKLYEHAVAHSGLPD
                     NARR"
     CDS             7858..7914
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
     CDS             join(7930..7932,7933..7935,7936..8646)
                     /codon_start=1
                     /product="enhanced YFP"
                     /label=EYFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTFGYGLQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSYQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(8236..8255)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     protein_bind    complement(9067..9100)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     misc_feature    9156..9744
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(9209..9229)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(9627..9638)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(9747..9763)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     LTR             9986..10166
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     rep_origin      complement(10228..10816)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(10308..10327)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(10987..11778,11779..11847))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     11610..11629
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(11848..11952)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 actagttatt aatagtaatc aattacgggg tcattagttc atagcccata tatggagttc
       61 cgcgttacat aacttacggt aaatggcccg cctggctgac cgcccaacga cccccgccca
      121 ttgacgtcaa taatgacgta tgttcccata gtaacgccaa tagggacttt ccattgacgt
      181 caatgggtgg agtatttacg gtaaactgcc cacttggcag tacatcaagt gtatcatatg
      241 ccaagtacgc cccctattga cgtcaatgac ggtaaatggc ccgcctggca ttatgcccag
      301 tacatgacct tatgggactt tcctacttgg cagtacatct acgtattagt catcgctatt
      361 accatggtga tgcggttttg gcagtacatc aatgggcgtg gatagcggtt tgactcacgg
      421 ggatttccaa gtctccaccc cattgacgtc aatgggagtt tgttttggca ccaaaatcaa
      481 cgggactttc caaaatgtcg taacaactcc gccccattga cgcaaatggg cggtaggcgt
      541 gtacggtggg aggtctatat aagcagcgcg ttttgcctgt actgggtctc tctggttaga
      601 ccagatctga gcctgggagc tctctggcta actagggaac ccactgctta agcctcaata
      661 aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta
      721 gagatccctc agaccctttt agtcagtgtg gaaaatctct agcagtggcg cccgaacagg
      781 gacttgaaag cgaaagggaa accagaggag ctctctcgac gcaggactcg gcttgctgaa
      841 gcgcgcacgg caagaggcga ggggcggcga ctggtgagta cgccaaaaat tttgactagc
      901 ggaggctaga aggagagaga tgggtgcgag agcgtcagta ttaagcgggg gagaattaga
      961 tcgcgatggg aaaaaattcg gttaaggcca gggggaaaga aaaaatataa attaaaacat
     1021 atagtatggg caagcaggga gctagaacga ttcgcagtta atcctggcct gttagaaaca
     1081 tcagaaggct gtagacaaat actgggacag ctacaaccat cccttcagac aggatcagaa
     1141 gaacttagat cattatataa tacagtagca accctctatt gtgtgcatca aaggatagag
     1201 ataaaagaca ccaaggaagc tttagacaag atagaggaag agcaaaacaa aagtaagacc
     1261 accgcacagc aagcggccgg ccgctgatct tcagacctgg aggaggagat atgagggaca
     1321 attggagaag tgaattatat aaatataaag tagtaaaaat tgaaccatta ggagtagcac
     1381 ccaccaaggc aaagagaaga gtggtgcaga gagaaaaaag agcagtggga ataggagctt
     1441 tgttccttgg gttcttggga gcagcaggaa gcactatggg cgcagcgtca atgacgctga
     1501 cggtacaggc cagacaatta ttgtctggta tagtgcagca gcagaacaat ttgctgaggg
     1561 ctattgaggc gcaacagcat ctgttgcaac tcacagtctg gggcatcaag cagctccagg
     1621 caagaatcct ggctgtggaa agatacctaa aggatcaaca gctcctgggg atttggggtt
     1681 gctctggaaa actcatttgc accactgctg tgccttggaa tgctagttgg agtaataaat
     1741 ctctggaaca gatttggaat cacacgacct ggatggagtg ggacagagaa attaacaatt
     1801 acacaagctt aatacactcc ttaattgaag aatcgcaaaa ccagcaagaa aagaatgaac
     1861 aagaattatt ggaattagat aaatgggcaa gtttgtggaa ttggtttaac ataacaaatt
     1921 ggctgtggta tataaaatta ttcataatga tagtaggagg cttggtaggt ttaagaatag
     1981 tttttgctgt actttctata gtgaatagag ttaggcaggg atattcacca ttatcgtttc
     2041 agacccacct cccaaccccg aggggacccg acaggcccga aggaatagaa gaagaaggtg
     2101 gagagagaga cagagacaga tccattcgat tagtgaacgg atcggcactg cgtgcgccaa
     2161 ttctgcagac aaatggcagt attcatccac aattttaaaa gaaaaggggg gattgggggg
     2221 tacagtgcag gggaaagaat agtagacata atagcaacag acatacaaac taaagaatta
     2281 caaaaacaaa ttacaaaaat tcaaaatttt cgggtttatt acagggacag cagagatcca
     2341 gtttggttag taccgggccc gctctagaga tccgacgcgc catctctagg cccgcgccgg
     2401 ccccctcgca cagacttgtg ggagaagctc ggctactccc ctgccccggt taatttgcat
     2461 ataatatttc ctagtaacta tagaggctta atgtgcgata aaagacagat aatctgttct
     2521 ttttaatact agctacattt tacatgatag gcttggattt ctataagaga tacaaatact
     2581 aaattattat tttaaaaaac agcacaaaag gaaactcacc ctaactgtaa agtaattgtg
     2641 tgttttgaga ctataaatat cccttggaga aaagccttgt taacgcgcgg tgaccctcga
     2701 ggtcgacggt atcgataagc tcgcttcacg agattccagc aggtcgaggg acctaataac
     2761 ttcgtatagc atacattata cgaagttata ttaagggttc caagcttaag cggccgcgtg
     2821 gataaccgta ttaccgccat gcattagtta ttaatagtaa tcaattacgg ggtcattagt
     2881 tcatagccca tatatggagt tccgcgttac ataacttacg gtaaatggcc cgcctggctg
     2941 accgcccaac gacccccgcc cattgacgtc aataatgacg tatgttccca tagtaacgcc
     3001 aatagggact ttccattgac gtcaatgggt ggagtattta cggtaaactg cccacttggc
     3061 agtacatcaa gtgtatcata tgccaagtac gccccctatt gacgtcaatg acggtaaatg
     3121 gcccgcctgg cattatgccc agtacatgac cttatgggac tttcctactt ggcagtacat
     3181 ctacgtatta gtcatcgcta ttaccatggt gatgcggttt tggcagtaca tcaatgggcg
     3241 tggatagcgg tttgactcac ggggatttcc aagtctccac cccattgacg tcaatgggag
     3301 tttgttttgg caccaaaatc aacgggactt tccaaaatgt cgtaacaact ccgccccatt
     3361 gacgcaaatg ggcggtaggc gtgtacggtg ggaggtctat ataagcagag ctggtttagt
     3421 gaaccgtcag atccgctagc accatggtga gcaagggcga ggagctgttc accggggtgg
     3481 tgcccatcct ggtcgagctg gacggcgacg taaacggcca caagttcagc gtgcgcggcg
     3541 agggcgaggg cgatgccacc aacggcaagc tgaccatgaa gttcatctgc accaccggca
     3601 agctgccagt gccctggccc accctcgtga ccaccctgac ctacggcgtg ctgtgcttcg
     3661 cccgctaccc cgaccacatg aagcagcacg acttcttcaa gtccgccatg cccgaaggct
     3721 acgtccagga gcgcaccatc agcttcgagg acgacggcta ttacaagacc cgcgccgagg
     3781 tgaagttcga gggcgacacc ctggtgaacc gcatcgagct gaagggcatc gacttcaagg
     3841 aggacggcaa catcctgggg cacaagctgg agtacaacct caacagccac aacgcctata
     3901 tcacggccga caagcagaag aacggcatca ggtctaactt caagatccgc ctcaacgtcg
     3961 aggacggcag cgtgcagctc gccgaccact accagcagaa cacccccatc ggcgacggcc
     4021 ccgtgctgct gcccgacaac cactacctga gcacccagaa taagctgagc aaagacccca
     4081 acgagaagcg cgatcacatg gtcctgctgg agttcgtgac cgcctccggg atcactctcg
     4141 gcatggacga gctgtacaag ctgcaagttc aactggtgga gtcaggtggt gcacttgttc
     4201 agccgggcgg tagtttgcga ctttcatgcg cggcgtcagg tttccctgta aaccgataca
     4261 gtatgaggtg gtatagacaa gcgcccggca aagaacgaga atgggttgca ggaatgagta
     4321 gtgcaggaga tcgaagttcc tacgaagaca gcgttaaagg gcgcttcact atcagtcgag
     4381 atgatgcacg gaacaccgtg tatctgcaaa tgaacagcct taaacctgaa gatacggccg
     4441 tatattattg taacgtcaac gtcggatttg aatactgggg acagggtacg caagtcacgg
     4501 tatctgatat ccatgcacac tggcggccgc tcgagatgaa tcccagtatg aagcagaaac
     4561 aagaagaaat caaagagaat ataaagaata gttctgtccc aagaagaact ctgaagatga
     4621 ttcagccttc tgcatctgga tctcttgttg gaagagaaaa tgagctgtcc gcaggcttgt
     4681 ccaaaaggaa acatcggaat gaccacttaa catctacaac ttccagccct ggggttattg
     4741 tcccagaatc tagtgaaaat aaaaatcttg gaggagtcac ccaggagtca tttgatctta
     4801 tgattaaaga aaatccatcc tctcagtatt ggaaggaagt ggcagaaaaa cggagaaagg
     4861 cgctgggttc cggagctact aacttcagcc tgctgaagca ggctggagac gtggaggaga
     4921 accctggacc tagatctggt ggctccatgg tgagtgtgat taaaccagac atgaagatca
     4981 agctgcgtat ggaaggcgct gtaaatggac acccgttcgc gattgaagga gttggccttg
     5041 ggaagccttt cgagggaaaa cagagtatgg accttaaagt caaagaaggc ggacctctgc
     5101 ctttcgccta tgacatcttg acaactgtgt tctgttacgg caacagggta ttcgccaaat
     5161 acccagaaaa tatagtagac tatttcaagc agtcgtttcc tgagggctac tcttgggaac
     5221 gaagcatgaa ttacgaagac gggggcattt gtaacgcgac aaacgacata accctggatg
     5281 gtgactgtta tatctatgaa attcgatttg atggtgtgaa ctttcctgcc aatggtccag
     5341 ttatgcagaa gaggactgtg aaatgggagc catccactga gaaattgtat gtgcgtgatg
     5401 gagtgctgaa gggtgatgtt aacatggctc tgtcgcttga aggaggtggc cattaccgat
     5461 gtgacttcaa aactacttat aaagctaaga aggttgtcca gttgccagac tatcactttg
     5521 tggaccacca cattgagatt aaaagccacg acaaagatta cagtaatgtt aatctgcatg
     5581 agcacgccga agcgcattct gagctgccga ggcaggccaa gtactcaatg ttgccttccc
     5641 tgggcgatat ccatgcacac tggcggccgc tcgagcccag ccccgccagg cccgcactcc
     5701 gcgccccggc ctccgctacc agtggcagcc gcaagcgcgc ccgcccgccc gccgcccccg
     5761 gacgcgacca ggccaggcca ccggcccgca ggagactgcg gctgtcggtg gacgaggttt
     5821 ccagccccag tacccccgag gccccagaca tcccagcctg cccttctccg ggccagaaga
     5881 taaagaaatc caccccggca gcaggtcagc cgccccacct gacatccgcg caggaccagg
     5941 acaccatcta aagaaggatc ttagcttaag gcccctctcc ctcccccccc cctaacgtta
     6001 ctggccgaag ccgcttggaa taaggccggt gtgcgtttgt ctatatgtta ttttccacca
     6061 tattgccgtc ttttggcaat gtgagggccc ggaaacctgg ccctgtcttc ttgacgagca
     6121 ttcctagggg tctttcccct ctcgccaaag gaatgcaagg tctgttgaat gtcgtgaagg
     6181 aagcagttcc tctggaagct tcttgaagac aaacaacgtc tgtagcgacc ctttgcaggc
     6241 agcggaaccc cccacctggc gacaggtgcc tctgcggcca aaagccacgt gtataagata
     6301 cacctgcaaa ggcggcacaa ccccagtgcc acgttgtgag ttggatagtt gtggaaagag
     6361 tcaaatggct ctcctcaagc gtattcaaca aggggctgaa ggatgcccag aaggtacccc
     6421 attgtatggg atctgatctg gggcctcggt gcacatgctt tacatgtgtt tagtcgaggt
     6481 taaaaaacgt ctaggccccc cgaaccacgg ggacgtggtt ttcctttgaa aaacacgatg
     6541 ataatggcca cagaattcat gaccgagaac tccacctccg ccccggcggc gaagcccaaa
     6601 cgggccaagg cttccaagaa gtccacggac caccccaagt attcagacat gatcgtggct
     6661 gctatccagg cagagaagaa ccgtgccggc tcctcgcgcc agtccatcca aaagtatatc
     6721 aagagccact acaaggtggg tgagaacgcc gactcccaga tcaagttgtc catcaagcgc
     6781 ctagtgacca ccggtgttct caagcaaacc aaaggggtgg gcgcctcggg gtccttcagg
     6841 ctggccaagg gcgatgagcc caaaaggtcg gtggctttca agaagaccaa gaaggaagtc
     6901 aagaaagtgg ccactccaaa gaaggcagcc aagcccaaga aggctgcctc caaagcccca
     6961 agcaagaaac ccaaagccac ccctgtcaag aaggccaaga agaagccggc tgccacgccc
     7021 aagaaagcca aaaagcccaa ggttgtcaaa gtcaaaccag tcaaggcctc caaacccaag
     7081 aaggccaaaa ccgtgaagcc caaagccaag tcgagtgcca agagggggag caagaagaag
     7141 tccggactca gatctggagg ctccggaggc atgagtgcga ttaagccaga catgaagatc
     7201 aaactccgta tggaaggcaa cgtaaacggg caccactttg tgatcgacgg agatggtaca
     7261 ggcaagcctt ttgagggaaa acagagtatg gatcttgaag tcaaagaggg cggacctctg
     7321 ccttttgcct ttgatatcct gaccactgca ttcgcgtacg gcaacagggt attcgccaaa
     7381 tatccagaca acatacaaga ctattttaag cagtcgtttc ctaaggggta ttcgtgggaa
     7441 cgaagcttga ctttcgaaga cgggggcatt tgcaacgcca gaaacgacat aacaatggaa
     7501 ggggacactt tctataataa agttcgattt tatggtacca actttcccgc caatggtcca
     7561 gttatgcaga agaagacgct gaaatgggag ccctccactg agaaaatgta tgtgcgtgat
     7621 ggagtgctga cgggtgatgt agagatggct ttgttgcttg aaggaaatgc ccattaccga
     7681 tgtgacttca gaactactta caaagctaag gagaagggtg tcaagttacc aggcgcccac
     7741 tttgtggacc actgcattga gattttaagc catgacaaag attacaacaa ggttaagctg
     7801 tatgagcatg ctgttgctca ttctggattg cctgacaatg ccagacgagg ttccggagct
     7861 actaacttca gcctgctgaa gcaggctgga gacgtggagg agaaccctgg acctagatct
     7921 ggtggctcca tggtgagcaa gggcgaggag ctgttcaccg gggtggtgcc catcctggtc
     7981 gagctggacg gcgacgtaaa cggccacaag ttcagcgtgt ccggcgaggg cgagggcgat
     8041 gccacctacg gcaagctgac cctgaagttc atctgcacca ccggcaagct gcccgtgccc
     8101 tggcccaccc tcgtgaccac cttcggctac ggcctgcagt gcttcgcccg ctaccccgac
     8161 cacatgaagc agcacgactt cttcaagtcc gccatgcccg aaggctacgt ccaggagcgc
     8221 accatcttct tcaaggacga cggcaactac aagacccgcg ccgaggtgaa gttcgagggc
     8281 gacaccctgg tgaaccgcat cgagctgaag ggcatcgact tcaaggagga cggcaacatc
     8341 ctggggcaca agctggagta caactacaac agccacaacg tctatatcat ggccgacaag
     8401 cagaagaacg gcatcaaggt gaacttcaag atccgccaca acatcgagga cggcagcgtg
     8461 cagctcgccg accactacca gcagaacacc cccatcggcg acggccccgt gctgctgccc
     8521 gacaaccact acctgagcta ccagtccgcc ctgagcaaag accccaacga gaagcgcgat
     8581 cacatggtcc tgctggagtt cgtgaccgcc gccgggatca ctctcggcat ggacgagctg
     8641 tacaagtact caatgttgcc ttccctggga ggatacccat cacactggcg accactcgag
     8701 gccagcccgc cgtcccccgc gcgatggagc ctgggacgga agcgcagagc cgacggcagg
     8761 cgctggaggc ccgaagacgc cgaggaggca gagcaccgcg gcgccgagcg cagacccgag
     8821 agctttacca ctcctgaagg ccctaaaccc cgttccagat gctctgactg ggcaagtgca
     8881 gttgaagaag atgaaatgag gaccagagtt aacaaagaaa tggcaagata taaaaggaaa
     8941 ctcctcatca atgactttgg aagagagaga aaatcatcat caggaagttc tgattcaaag
     9001 gagtctatgt ctactgtgcc ggctgacgat cgaggatgag tttaaacgaa ttcgtcgagg
     9061 gacctaataa cttcgtatag catacattat acgaagttat acatgtttaa gggttccggt
     9121 tccactaggt acaattcgat atcaagctta tcgataatca acctctggat tacaaaattt
     9181 gtgaaagatt gactggtatt cttaactatg ttgctccttt tacgctatgt ggatacgctg
     9241 ctttaatgcc tttgtatcat gctattgctt cccgtatggc tttcattttc tcctccttgt
     9301 ataaatcctg gttgctgtct ctttatgagg agttgtggcc cgttgtcagg caacgtggcg
     9361 tggtgtgcac tgtgtttgct gacgcaaccc ccactggttg gggcattgcc accacctgtc
     9421 agctcctttc cgggactttc gctttccccc tccctattgc cacggcggaa ctcatcgccg
     9481 cctgccttgc ccgctgctgg acaggggctc ggctgttggg cactgacaat tccgtggtgt
     9541 tgtcggggaa atcatcgtcc tttccttggc tgctcgcctg tgttgccacc tggattctgc
     9601 gcgggacgtc cttctgctac gtcccttcgg ccctcaatcc agcggacctt ccttcccgcg
     9661 gcctgctgcc ggctctgcgg cctcttccgc gtcttcgcct tcgccctcag acgagtcgga
     9721 tctccctttg ggccgcctcc ccgcatcgat accgtcgacc tcgatcgaga cctagaaaaa
     9781 catggagcaa tcacaagtag caatacagca gctaccaatg ctgattgtgc ctggctagaa
     9841 gcacaagagg aggaggaggt gggttttcca gtcacacctc aggtaccttt aagaccaatg
     9901 acttacaagg cagctgacgc gtgattttaa aagaaaaggg gggactggaa gggctaattc
     9961 actcccactg ctttttgcct gtactgggtc tctctggtta gaccagatct gagcctggga
    10021 gctctctggc taactaggga acccactgct taagcctcaa taaagcttgc cttgagtgct
    10081 tcaagtagtg tgtgcccgtc tgttgtgtga ctctggtaac tagagatccc tcagaccctt
    10141 ttagtcagtg tggaaaatct ctagcagcat gtgagcaaaa ggccagcaaa aggccaggaa
    10201 ccgtaaaaag gccgcgttgc tggcgttttt ccataggctc cgcccccctg acgagcatca
    10261 caaaaatcga cgctcaagtc agaggtggcg aaacccgaca ggactataaa gataccaggc
    10321 gtttccccct ggaagctccc tcgtgcgctc tcctgttccg accctgccgc ttaccggata
    10381 cctgtccgcc tttctccctt cgggaagcgt ggcgctttct catagctcac gctgtaggta
    10441 tctcagttcg gtgtaggtcg ttcgctccaa gctgggctgt gtgcacgaac cccccgttca
    10501 gcccgaccgc tgcgccttat ccggtaacta tcgtcttgag tccaacccgg taagacacga
    10561 cttatcgcca ctggcagcag ccactggtaa caggattagc agagcgaggt atgtaggcgg
    10621 tgctacagag ttcttgaagt ggtggcctaa ctacggctac actagaagaa cagtatttgg
    10681 tatctgcgct ctgctgaagc cagttacctt cggaaaaaga gttggtagct cttgatccgg
    10741 caaacaaacc accgctggta gcggtggttt ttttgtttgc aagcagcaga ttacgcgcag
    10801 aaaaaaagga tctcaagaag atcctttgat cttttctacg gggtctgacg ctcagtggaa
    10861 cgaaaactca cgttaaggga ttttggtcat gagattatca aaaaggatct tcacctagat
    10921 ccttttaaat taaaaatgaa gttttaaatc aatctaaagt atatatgagt aaacttggtc
    10981 tgacagttac caatgcttaa tcagtgaggc acctatctca gcgatctgtc tatttcgttc
    11041 atccatagtt gcctgactcc ccgtcgtgta gataactacg atacgggagg gcttaccatc
    11101 tggccccagt gctgcaatga taccgcgaga cccacgctca ccggctccag atttatcagc
    11161 aataaaccag ccagccggaa gggccgagcg cagaagtggt cctgcaactt tatccgcctc
    11221 catccagtct attaattgtt gccgggaagc tagagtaagt agttcgccag ttaatagttt
    11281 gcgcaacgtt gttgccattg ctacaggcat cgtggtgtca cgctcgtcgt ttggtatggc
    11341 ttcattcagc tccggttccc aacgatcaag gcgagttaca tgatccccca tgttgtgcaa
    11401 aaaagcggtt agctccttcg gtcctccgat cgttgtcaga agtaagttgg ccgcagtgtt
    11461 atcactcatg gttatggcag cactgcataa ttctcttact gtcatgccat ccgtaagatg
    11521 cttttctgtg actggtgagt actcaaccaa gtcattctga gaatagtgta tgcggcgacc
    11581 gagttgctct tgcccggcgt caatacggga taataccgcg ccacatagca gaactttaaa
    11641 agtgctcatc attggaaaac gttcttcggg gcgaaaactc tcaaggatct taccgctgtt
    11701 gagatccagt tcgatgtaac ccactcgtgc acccaactga tcttcagcat cttttacttt
    11761 caccagcgtt tctgggtgag caaaaacagg aaggcaaaat gccgcaaaaa agggaataag
    11821 ggcgacacgg aaatgttgaa tactcatact cttccttttt caatattatt gaagcattta
    11881 tcagggttat tgtctcatga gcggatacat atttgaatgt atttagaaaa ataaacaaat
    11941 aggggttccg cgcacatttc cccgaaaagt gccacctgac
//
