LOCUS       sequence-410835-        9509 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Mammalian expression of the human LTB4 receptor LTB4R with the T308A
            and S310A mutations..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9509)
  AUTHORS   Lundgren SM, Rocha-Gregg BL, Akdogan E, Mysore MN, Hayes S, Collins 
            SR
  TITLE     Signaling dynamics distinguish high- and low-priority neutrophil 
            chemoattractant receptors.
  JOURNAL   Sci Signal. 2023 Oct 3;16(805):eadd1845. doi: 
            10.1126/scisignal.add1845. Epub 2023 Oct 3.
  PUBMED    37788324
REFERENCE   2  (bases 1 to 9509)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Sci 
            Signal."; date: "2023-10-3"; volume: "16(805):eadd1845. doi"; pages:
            " 10.1126/scisignal.add1845. Epub 2023 Oct 3"
COMMENT     Sequence Label: pLV_PGK_LTB4R(T308A,S310A)
FEATURES             Location/Qualifiers
     source          1..9509
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    1..234
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             419..463
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    726..841
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1 (lacking the first T)"
     promoter        887..1397
                     /label=hPGK promoter
                     /note="human phosphoglycerate kinase 1 promoter"
     primer_bind     1316..1334
                     /label=hPGK-F
                     /note="Human PGK promoter, forward primer"
     misc_feature    2575..3124
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(2719..2736)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     2946..2965
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     CDS             3127..4152
                     /codon_start=1
                     /product="hygromycin B phosphotransferase"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
                     E"
     CDS             3127..4152
                     /codon_start=1
                     /gene="aph(4)-Ia"
                     /product="aminoglycoside phosphotransferase from E. coli"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
                     E"
     misc_feature    4176..4764
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(4229..4249)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(4647..4658)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             4971..5604
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     primer_bind     complement(5732..5748)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(5732..5748)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(5745..5767)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    5756..5772
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(5780..5786,5787..5804,5805..5810))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    5825..5846
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(5963..5980)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(6134..6722)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(6214..6233)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(6893..7684,7685..7753))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     7516..7535
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(7754..7858)
                     /gene="bla"
                     /label=AmpR promoter
     polyA_signal    7906..8040
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(7943..7962)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     7997..8016
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     LTR             8208..8841
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     misc_feature    8888..9013
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
ORIGIN
        1 aggagctttg ttccttgggt tcttgggagc agcaggaagc actatgggcg cagcgtcaat
       61 gacgctgacg gtacaggcca gacaattatt gtctggtata gtgcagcagc agaacaattt
      121 gctgagggct attgaggcgc aacagcatct gttgcaactc acagtctggg gcatcaagca
      181 gctccaggca agaatcctgg ctgtggaaag atacctaaag gatcaacagc tcctggggat
      241 ttggggttgc tctggaaaac tcatttgcac cactgctgtg ccttggaatg ctagttggag
      301 taataaatct ctggaacaga tttggaatca cacgacctgg atggagtggg acagagaaat
      361 taacaattac acaagcttaa tacactcctt aattgaagaa tcgcaaaacc agcaagaaaa
      421 gaatgaacaa gaattattgg aattagataa atgggcaagt ttgtggaatt ggtttaacat
      481 aacaaattgg ctgtggtata taaaattatt cataatgata gtaggaggct tggtaggttt
      541 aagaatagtt tttgctgtac tttctatagt gaatagagtt aggcagggat attcaccatt
      601 atcgtttcag acccacctcc caaccccgag gggacccgac aggcccgaag gaatagaaga
      661 agaaggtgga gagagagaca gagacagatc cattcgatta gtgaacggat ctcgacggta
      721 tcgcctttaa aagaaaaggg gggattgggg ggtacagtgc aggggaaaga atagtagaca
      781 taatagcaac agacatacaa actaaagaat tacaaaaaca aattacaaaa attcaaaatt
      841 ttcgggttta ttacagggac agcagagatc cagtttatcg atccacgggg ttggggttgc
      901 gccttttcca aggcagccct gggtttgcgc agggacgcgg ctgctctggg cgtggttccg
      961 ggaaacgcag cggcgccgac cctgggtctc gcacattctt cacgtccgtt cgcagcgtca
     1021 cccggatctt cgccgctacc cttgtgggcc ccccggcgac gcttcctgct ccgcccctaa
     1081 gtcgggaagg ttccttgcgg ttcgcggcgt gccggacgtg acaaacggaa gccgcacgtc
     1141 tcactagtac cctcgcagac ggacagcgcc agggagcaat ggcagcgcgc cgaccgcgat
     1201 gggctgtggc caatagcggc tgctcagcag ggcgcgccga gagcagcggc cgggaagggg
     1261 cggtgcggga ggcggggtgt ggggcggtag tgtgggccct gttcctgccc gcgcggtgtt
     1321 ccgcattctg caagcctccg gagcgcacgt cggcagtcgg ctccctcgtt gaccgaatca
     1381 ccgacctctc tccccaggac gcgggatccg ccaccatgaa cactacatct tctgcagcac
     1441 ccccctcact aggtgtagag ttcatctctc tgctggctat catcctgctg tcagtggcgc
     1501 tggctgtggg gcttcccggc aacagctttg tggtgtggag tatcctgaaa aggatgcaga
     1561 agcgctctgt cactgccctg atggtgctga acctggccct ggccgacctg gccgtattgc
     1621 tcactgctcc ctttttcctt cacttcctgg cccaaggcac ctggagtttt ggactggctg
     1681 gttgccgcct gtgtcactat gtctgcggag tcagcatgta cgccagcgtc ctgcttatca
     1741 cggccatgag tctagaccgc tcactggcgg tggcccgccc ctttgtgtcc cagaagctac
     1801 gcaccaaggc gatggcccgg cgggtgctgg caggcatctg ggtgttgtcc tttctgctgg
     1861 ccacacccgt cctcgcgtac cgcacagtag tgccctggaa aacgaacatg agcctgtgct
     1921 tcccgcggta ccccagcgaa gggcaccggg ccttccatct aatcttcgag gctgtcacgg
     1981 gcttcctgct gcccttcctg gctgtggtgg ccagctactc ggacataggg cgtcggctac
     2041 aggcccggcg cttccgccgc agccgccgca ccggccgcct ggtggtgctc atcatcctga
     2101 ccttcgccgc cttctggctg ccctaccacg tggtgaacct ggctgaggcg ggccgcgcgc
     2161 tggccggcca ggccgccggg ttagggctcg tggggaagcg gctgagcctg gcccgcaacg
     2221 tgctcatcgc actcgccttc ctgagcagca gcgtgaaccc cgtgctgtac gcgtgcgccg
     2281 gcggcggcct gctgcgctcg gcgggcgtgg gcttcgtcgc caagctgctg gagggcgcgg
     2341 gcgccgaggc gtccagcacg cgccgcgggg gcagcctggg ccagaccgct aggagcggcc
     2401 ccgccgctct ggagcccggc ccttccgaga gcctcactgc ctccagccct ctcaagttaa
     2461 acgaactgaa ctaagcggcc gctctagagt cgacgggccg cggtaacaat tgttaactaa
     2521 cttaagctag caacggtttc cctctagcgg gatcaattcc gccccccccc cctaacgtta
     2581 ctggccgaag ccgcttggaa taaggccggt gtgcgtttgt ctatatgtta ttttccacca
     2641 tattgccgtc ttttggcaat gtgagggccc ggaaacctgg ccctgtcttc ttgacgagca
     2701 ttcctagggg tctttcccct ctcgccaaag gaatgcaagg tctgttgaat gtcgtgaagg
     2761 aagcagttcc tctggaagct tcttgaagac aaacaacgtc tgtagcgacc ctttgcaggc
     2821 agcggaaccc cccacctggc gacaggtgcc tctgcggcca aaagccacgt gtataagata
     2881 cacctgcaaa ggcggcacaa ccccagtgcc acgttgtgag ttggatagtt gtggaaagag
     2941 tcaaatggct ctcctcaagc gtattcaaca aggggctgaa ggatgcccag aaggtacccc
     3001 attgtatggg atctgatctg gggcctcggt gcacatgctt tacatgtgtt tagtcgaggt
     3061 taaaaaaacg tctaggcccc ccgaaccacg gggacgtggt tttcctttga aaaacacgat
     3121 aataccatga aaaagcctga actcaccgcg acgtctgtcg agaagtttct gatcgaaaag
     3181 ttcgacagcg tctccgacct gatgcagctc tcggagggcg aagaatctcg tgctttcagc
     3241 ttcgatgtag gagggcgtgg atatgtcctg cgggtaaata gctgcgccga tggtttctac
     3301 aaagatcgtt atgtttatcg gcactttgca tcggccgcgc tcccgattcc ggaagtgctt
     3361 gacattgggg aattcagcga gagcctgacc tattgcatct cccgccgtgc acagggtgtc
     3421 acgttgcaag acctgcctga aaccgaactg cccgctgttc tgcagccggt cgcggaggcc
     3481 atggatgcga tcgctgcggc cgatcttagc cagacgagcg ggttcggccc attcggaccg
     3541 caaggaatcg gtcaatacac tacatggcgt gatttcatat gcgcgattgc tgatccccat
     3601 gtgtatcact ggcaaactgt gatggacgac accgtcagtg cgtccgtcgc gcaggctctc
     3661 gatgagctga tgctttgggc cgaggactgc cccgaagtcc ggcacctcgt gcacgcggat
     3721 ttcggctcca acaatgtcct gacggacaat ggccgcataa cagcggtcat tgactggagc
     3781 gaggcgatgt tcggggattc ccaatacgag gtcgccaaca tcttcttctg gaggccgtgg
     3841 ttggcttgta tggagcagca gacgcgctac ttcgagcgga ggcatccgga gcttgcagga
     3901 tcgccgcggc tccgggcgta tatgctccgc attggtcttg accaactcta tcagagcttg
     3961 gttgacggca atttcgatga tgcagcttgg gcgcagggtc gatgcgacgc aatcgtccga
     4021 tccggagccg ggactgtcgg gcgtacacaa atcgcccgca gaagcgcggc cgtctggacc
     4081 gatggctgtg tagaagtact cgccgatagt ggaaaccgac gccccagcac tcgtccgagg
     4141 gcaaaggaat agcccgggcg gggcgcgtct ggaacaatca acctctggat tacaaaattt
     4201 gtgaaagatt gactggtatt cttaactatg ttgctccttt tacgctatgt ggatacgctg
     4261 ctttaatgcc tttgtatcat gctattgctt cccgtatggc tttcattttc tcctccttgt
     4321 ataaatcctg gttgctgtct ctttatgagg agttgtggcc cgttgtcagg caacgtggcg
     4381 tggtgtgcac tgtgtttgct gacgcaaccc ccactggttg gggcattgcc accacctgtc
     4441 agctcctttc cgggactttc gctttccccc tccctattgc cacggcggaa ctcatcgccg
     4501 cctgccttgc ccgctgctgg acaggggctc ggctgttggg cactgacaat tccgtggtgt
     4561 tgtcggggaa gctgacgtcc tttccatggc tgctcgcctg tgttgccacc tggattctgc
     4621 gcgggacgtc cttctgctac gtcccttcgg ccctcaatcc agcggacctt ccttcccgcg
     4681 gcctgctgcc ggctctgcgg cctcttccgc gtcttcgcct tcgccctcag acgagtcgga
     4741 tctccctttg ggccgcctcc ccgcctggaa ttaattctgc agtcgagacc tagaaaaaca
     4801 tggagcaatc acaagtagca atacagcagc taccaatgct gattgtgcct ggctagaagc
     4861 acaagaggag gaggaggtgg gttttccagt cacacctcag gtacctttaa gaccaatgac
     4921 ttacaaggca gctgtagatc ttagccactt tttaaaagaa aagaggggac tggaagggct
     4981 aattcactcc caacgaagac aagatatcct tgatctgtgg atctaccaca cacaaggcta
     5041 cttccctgat tagcagaact acacaccagg gccaggggtc agatatccac tgacctttgg
     5101 atggtgctac aagctagtac cagttgagcc agataaggta gaagaggcca ataaaggaga
     5161 gaacaccagc ttgttacacc ctgtgagcct gcatgggatg gatgacccgg agagagaagt
     5221 gttagagtgg aggtttgaca gccgcctagc atttcatcac gtggcccgag agctgcatcc
     5281 ggagtacttc aagaactgct gatatcgagc ttgctacaag ggactttccg ctggggactt
     5341 tccagggagg cgtggcctgg gcgggactgg ggagtggcga gccctcagat cctgcatata
     5401 agcagctgct ttttgcctgt actgggtctc tctggttaga ccagatctga gcctgggagc
     5461 tctctggcta actagggaac ccactgctta agcctcaata aagcttgcct tgagtgcttc
     5521 aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta gagatccctc agaccctttt
     5581 agtcagtgtg gaaaatctct agcagtagta gttcatgtca tcttattatt cagtatttat
     5641 aacttgcaaa gaaatgaata tcagagagtg agaggccttg acattgctag cgtttaccgt
     5701 cgacctctag ctagagcttg gcgtaatcat ggtcatagct gtttcctgtg tgaaattgtt
     5761 atccgctcac aattccacac aacatacgag ccggaagcat aaagtgtaaa gcctggggtg
     5821 cctaatgagt gagctaactc acattaattg cgttgcgctc actgcccgct ttccagtcgg
     5881 gaaacctgtc gtgccagctg cattaatgaa tcggccaacg cgcggggaga ggcggtttgc
     5941 gtattgggcg ctcttccgct tcctcgctca ctgactcgct gcgctcggtc gttcggctgc
     6001 ggcgagcggt atcagctcac tcaaaggcgg taatacggtt atccacagaa tcaggggata
     6061 acgcaggaaa gaacatgtga gcaaaaggcc agcaaaaggc caggaaccgt aaaaaggccg
     6121 cgttgctggc gtttttccat aggctccgcc cccctgacga gcatcacaaa aatcgacgct
     6181 caagtcagag gtggcgaaac ccgacaggac tataaagata ccaggcgttt ccccctggaa
     6241 gctccctcgt gcgctctcct gttccgaccc tgccgcttac cggatacctg tccgcctttc
     6301 tcccttcggg aagcgtggcg ctttctcata gctcacgctg taggtatctc agttcggtgt
     6361 aggtcgttcg ctccaagctg ggctgtgtgc acgaaccccc cgttcagccc gaccgctgcg
     6421 ccttatccgg taactatcgt cttgagtcca acccggtaag acacgactta tcgccactgg
     6481 cagcagccac tggtaacagg attagcagag cgaggtatgt aggcggtgct acagagttct
     6541 tgaagtggtg gcctaactac ggctacacta gaagaacagt atttggtatc tgcgctctgc
     6601 tgaagccagt taccttcgga aaaagagttg gtagctcttg atccggcaaa caaaccaccg
     6661 ctggtagcgg tggttttttt gtttgcaagc agcagattac gcgcagaaaa aaaggatctc
     6721 aagaagatcc tttgatcttt tctacggggt ctgacgctca gtggaacgaa aactcacgtt
     6781 aagggatttt ggtcatgaga ttatcaaaaa ggatcttcac ctagatcctt ttaaattaaa
     6841 aatgaagttt taaatcaatc taaagtatat atgagtaaac ttggtctgac agttaccaat
     6901 gcttaatcag tgaggcacct atctcagcga tctgtctatt tcgttcatcc atagttgcct
     6961 gactccccgt cgtgtagata actacgatac gggagggctt accatctggc cccagtgctg
     7021 caatgatacc gcgagaccca cgctcaccgg ctccagattt atcagcaata aaccagccag
     7081 ccggaagggc cgagcgcaga agtggtcctg caactttatc cgcctccatc cagtctatta
     7141 attgttgccg ggaagctaga gtaagtagtt cgccagttaa tagtttgcgc aacgttgttg
     7201 ccattgctac aggcatcgtg gtgtcacgct cgtcgtttgg tatggcttca ttcagctccg
     7261 gttcccaacg atcaaggcga gttacatgat cccccatgtt gtgcaaaaaa gcggttagct
     7321 ccttcggtcc tccgatcgtt gtcagaagta agttggccgc agtgttatca ctcatggtta
     7381 tggcagcact gcataattct cttactgtca tgccatccgt aagatgcttt tctgtgactg
     7441 gtgagtactc aaccaagtca ttctgagaat agtgtatgcg gcgaccgagt tgctcttgcc
     7501 cggcgtcaat acgggataat accgcgccac atagcagaac tttaaaagtg ctcatcattg
     7561 gaaaacgttc ttcggggcga aaactctcaa ggatcttacc gctgttgaga tccagttcga
     7621 tgtaacccac tcgtgcaccc aactgatctt cagcatcttt tactttcacc agcgtttctg
     7681 ggtgagcaaa aacaggaagg caaaatgccg caaaaaaggg aataagggcg acacggaaat
     7741 gttgaatact catactcttc ctttttcaat attattgaag catttatcag ggttattgtc
     7801 tcatgagcgg atacatattt gaatgtattt agaaaaataa acaaataggg gttccgcgca
     7861 catttccccg aaaagtgcca cctgacgtcg acggatcggg agatcaactt gtttattgca
     7921 gcttataatg gttacaaata aagcaatagc atcacaaatt tcacaaataa agcatttttt
     7981 tcactgcatt ctagttgtgg tttgtccaaa ctcatcaatg tatcttatca tgtctggatc
     8041 aactggataa ctcaagctaa ccaaaatcat cccaaacttc ccaccccata ccctattacc
     8101 actgccaatt acctgtggtt tcatttactc taaacctgtg attcctctga attattttca
     8161 ttttaaagaa attgtatttg ttaaatatgt actacaaact tagtagttgg aagggctaat
     8221 tcactcccaa agaagacaag atatccttga tctgtggatc taccacacac aaggctactt
     8281 ccctgattag cagaactaca caccagggcc aggggtcaga tatccactga cctttggatg
     8341 gtgctacaag ctagtaccag ttgagccaga taaggtagaa gaggccaata aaggagagaa
     8401 caccagcttg ttacaccctg tgagcctgca tgggatggat gacccggaga gagaagtgtt
     8461 agagtggagg tttgacagcc gcctagcatt tcatcacgtg gcccgagagc tgcatccgga
     8521 gtacttcaag aactgctgat atcgagcttg ctacaaggga ctttccgctg gggactttcc
     8581 agggaggcgt ggcctgggcg ggactgggga gtggcgagcc ctcagatcct gcatataagc
     8641 agctgctttt tgcctgtact gggtctctct ggttagacca gatctgagcc tgggagctct
     8701 ctggctaact agggaaccca ctgcttaagc ctcaataaag cttgccttga gtgcttcaag
     8761 tagtgtgtgc ccgtctgttg tgtgactctg gtaactagag atccctcaga cccttttagt
     8821 cagtgtggaa aatctctagc agtggcgccc gaacagggac ttgaaagcga aagggaaacc
     8881 agaggagctc tctcgacgca ggactcggct tgctgaagcg cgcacggcaa gaggcgaggg
     8941 gcggcgactg gtgagtacgc caaaaatttt gactagcgga ggctagaagg agagagatgg
     9001 gtgcgagagc gtcagtatta agcgggggag aattagatcg cgatgggaaa aaattcggtt
     9061 aaggccaggg ggaaagaaaa aatataaatt aaaacatata gtatgggcaa gcagggagct
     9121 agaacgattc gcagttaatc ctggcctgtt agaaacatca gaaggctgta gacaaatact
     9181 gggacagcta caaccatccc ttcagacagg atcagaagaa cttagatcat tatataatac
     9241 agtagcaacc ctctattgtg tgcatcaaag gatagagata aaagacacca aggaagcttt
     9301 agacaagata gaggaagagc aaaacaaaag taagaccacc gcacagcaag cggccggccg
     9361 ctgatcttca gacctggagg aggagatatg agggacaatt ggagaagtga attatataaa
     9421 tataaagtag taaaaattga accattagga gtagcaccca ccaaggcaaa gagaagagtg
     9481 gtgcagagag aaaaaagagc agtgggaat
//
