LOCUS       sequence-410847-        9087 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  CMV and T7 promoter expression plasmid for human codon optimized 
            CE-ABE8e-SpCas9 A-to-G base editor and P2A-EGFP.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9087)
  AUTHORS   Alves CRR, Ha LL, Yaworski R, Sutton ER, Lazzarotto CR, Christie KA,
            Reilly A, Beauvais A, Doll RM, de la Cruz D, Maguire CA, Swoboda KJ,
            Tsai SQ, Kothary R, Kleinstiver BP
  TITLE     Optimization of base editors for the functional correction of SMN2 
            as a treatment for spinal muscular atrophy.
  JOURNAL   Nat Biomed Eng. 2023 Dec 6. doi: 10.1038/s41551-023-01132-z.
  PUBMED    38057426
REFERENCE   2  (bases 1 to 9087)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat Biomed 
            Eng. 2023 Dec 6. doi: 10.1038/s41551-023-01132-z."
COMMENT     Sequence Label: pCMV-T7-BPNLS-CE-ABE8e-SpCas9(D10A)-BPNLS-P2A-...
FEATURES             Location/Qualifiers
     source          1..9087
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        132..335
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     286..306
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     377..396
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        377..395
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             445..465
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             472..2187
                     /codon_start=1
                     /product="N-terminal portion of Streptococcus pyogenes Cas9
                     (Zetsche et al., 2015)"
                     /label=Cas9(N)
                     /translation="DKKYSIGLAIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKKN
                     LIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEESF
                     LVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIKF
                     RGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRLE
                     NLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQI
                     GDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVRQ
                     QLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLLR
                     KQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARGN
                     SRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEYF
                     TVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIE"
     CDS             5257..5277
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             5287..5343
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
     CDS             join(5344..5346,5347..5349,5350..6060)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             join(5344..5346,5347..5349,5350..6060)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(5650..5669)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     5997..6018
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     CDS             6073..6093
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             6102..6119
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(6142..6159)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    6148..6372
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     primer_bind     complement(6443..6459)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(6443..6459)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(6456..6478)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    6467..6483
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(6491..6497,6498..6515,6516..6521))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    6536..6557
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(6674..6691)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(6845..7433)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(6925..6944)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(7604..8395,8396..8464))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     8227..8246
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(8465..8569)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(8648..8667)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        join(8839..9087,1..131)
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
ORIGIN
        1 atatgccaag tacgccccct attgacgtca atgacggtaa atggcccgcc tggcattatg
       61 cccagtacat gaccttatgg gactttccta cttggcagta catctacgta ttagtcatcg
      121 ctattaccat ggtgatgcgg ttttggcagt acatcaatgg gcgtggatag cggtttgact
      181 cacggggatt tccaagtctc caccccattg acgtcaatgg gagtttgttt tggcaccaaa
      241 atcaacggga ctttccaaaa tgtcgtaaca actccgcccc attgacgcaa atgggcggta
      301 ggcgtgtacg gtgggaggtc tatataagca gagctggttt agtgaaccgt cagatccgct
      361 agagatccgc ggccgctaat acgactcact atagggagag ccgccaccat gaaacggaca
      421 gccgacggaa gcgagttcga gtcaccaaag aagaagcgga aagtcagcag tgacaagaag
      481 tacagcatcg gcctggccat cggcaccaac tctgtgggct gggccgtgat caccgacgag
      541 tacaaggtgc ccagcaagaa attcaaggtg ctgggcaaca ccgaccggca cagcatcaag
      601 aagaacctga tcggagccct gctgttcgac agcggcgaaa cagccgaggc cacccggctg
      661 aagagaaccg ccagaagaag atacaccaga cggaagaacc ggatctgcta tctgcaagag
      721 atcttcagca acgagatggc caaggtggac gacagcttct tccacagact ggaagagtcc
      781 ttcctggtgg aagaggataa gaagcacgag cggcacccca tcttcggcaa catcgtggac
      841 gaggtggcct accacgagaa gtaccccacc atctaccacc tgagaaagaa actggtggac
      901 agcaccgaca aggccgacct gcggctgatc tatctggccc tggcccacat gatcaagttc
      961 cggggccact tcctgatcga gggcgacctg aaccccgaca acagcgacgt ggacaagctg
     1021 ttcatccagc tggtgcagac ctacaaccag ctgttcgagg aaaaccccat caacgccagc
     1081 ggcgtggacg ccaaggccat cctgtctgcc agactgagca agagcagacg gctggaaaat
     1141 ctgatcgccc agctgcccgg cgagaagaag aatggcctgt tcggaaacct gattgccctg
     1201 agcctgggcc tgacccccaa cttcaagagc aacttcgacc tggccgagga tgccaaactg
     1261 cagctgagca aggacaccta cgacgacgac ctggacaacc tgctggccca gatcggcgac
     1321 cagtacgccg acctgtttct ggccgccaag aacctgtccg acgccatcct gctgagcgac
     1381 atcctgagag tgaacaccga gatcaccaag gcccccctga gcgcctctat gatcaagaga
     1441 tacgacgagc accaccagga cctgaccctg ctgaaagctc tcgtgcggca gcagctgcct
     1501 gagaagtaca aagagatttt cttcgaccag agcaagaacg gctacgccgg ctacattgac
     1561 ggcggagcca gccaggaaga gttctacaag ttcatcaagc ccatcctgga aaagatggac
     1621 ggcaccgagg aactgctcgt gaagctgaac agagaggacc tgctgcggaa gcagcggacc
     1681 ttcgacaacg gcagcatccc ccaccagatc cacctgggag agctgcacgc cattctgcgg
     1741 cggcaggaag atttttaccc attcctgaag gacaaccggg aaaagatcga gaagatcctg
     1801 accttccgca tcccctacta cgtgggccct ctggccaggg gaaacagcag attcgcctgg
     1861 atgaccagaa agagcgagga aaccatcacc ccctggaact tcgaggaagt ggtggacaag
     1921 ggcgcttccg cccagagctt catcgagcgg atgaccaact tcgataagaa cctgcccaac
     1981 gagaaggtgc tgcccaagca cagcctgctg tacgagtact tcaccgtgta taacgagctg
     2041 accaaagtga aatacgtgac cgagggaatg agaaagcccg ccttcctgag cggcgagcag
     2101 aaaaaggcca tcgtggacct gctgttcaag accaaccgga aagtgaccgt gaagcagctg
     2161 aaagaggact acttcaagaa aatcgagtgc ttcgactccg tggaaatctc cggcgtggaa
     2221 gatcggttca acgcctccct gggcacatac cacgatctgc tgaaaattat caaggacaag
     2281 gacttcctgg acaatgagga aaacgaggac attctggaag atatcgtgct gaccctgaca
     2341 ctgtttgagg acagagagat gatcgaggaa cggctgaaaa cctatgccca cctgttcgac
     2401 gacaaagtga tgaagcagct gaagcggcgg agatacaccg gctggggcag gctgagccgg
     2461 aagctgatca acggcatccg ggacaagcag tccggcaaga caatcctgga tttcctgaag
     2521 tccgacggct tcgccaacag aaacttcatg cagctgatcc acgacgacag cctgaccttt
     2581 aaagaggaca tccagaaagc ccaggtgtcc ggccagggcg atagcctgca cgagcacatt
     2641 gccaatctgg ccggcagccc cgccattaag aagggcatcc tgcagacagt gaaggtggtg
     2701 gacgagctcg tgaaagtgat gggccggcac aagcccgaga acatcgtgat cgaaatggcc
     2761 agagagaacc agaccaccca gaagggacag aagaacagcc gcgagagaat gaagcggatc
     2821 gaagagggca tcaaagagct gggcagccag atcctgaaag aacaccccgt ggaaaacacc
     2881 cagctgcaga acgagaagct gtacctgtac tacctgcaga atgggcggga tatgtacgtg
     2941 gaccaggaac tggacatcaa ccggctgtcc gactacgatg tggaccatat cgtgcctcag
     3001 agctttctga aggacgactc catcgacaac aaggtgctga ccagaagcga caagaaccgg
     3061 ggcaagagcg acaacgtgcc ctccgaagag gtcgtgaaga agatgaagaa ctactggcgg
     3121 cagctgctga acgccaagct gattacccag agaaagttcg acaatctgac caaggccgag
     3181 agaggcggcc tgagcgaact ggataaggcc ggcttcatca agagacagct ggtggaaacc
     3241 cggcagatca caaagcacgt ggcacagatc ctggactccc ggatgaacac taagtacgac
     3301 gagaatgaca agctgatccg ggaagtgaaa gtgatcaccc tgaagtccaa gctggtgtcc
     3361 gatttccgga aggatttcca gttttacaaa gtgcgcgaga tcaacaacta ccaccacgcc
     3421 cacgacgcct acctgaacgc cgtcgtggga accgccctga tcaaaaagta ccctaagctg
     3481 gaaagcgagt tcgtgtacgg cgactacaag gtgtacgacg tgcggaagat gatcgccaag
     3541 agcgagcagg aaatcggcaa ggctaccgcc aagtacttct tctacagcaa catcatgaac
     3601 tttttcaagt ctggtggcag ttccggtggt tcttccggaa gcgaaactcc agggacaagc
     3661 gaatccgcca caccagagag ctccggcggc tcctccggag gatcctctga ggtggagttt
     3721 tcccacgagt actggatgag acatgccctg accctggcca agagggcacg cgatgagagg
     3781 gaggtgcctg tgggagccgt gctggtgctg aacaatagag tgatcggcga gggctggaac
     3841 agagccatcg gcctgcacga cccaacagcc catgccgaaa ttatggccct gagacagggc
     3901 ggcctggtca tgcagaacta cagactgatt gacgccaccc tgtacgtgac attcgagcct
     3961 tgcgtgatgt gcgccggcgc catgatccac tctaggatcg gccgcgtggt gtttggcgtg
     4021 aggaacagca aaagaggcgc cgcaggctcc ctgatgaacg tgctgaacta ccccggcatg
     4081 aatcaccgcg tcgaaattac cgagggaatc ctggcagatg aatgtgccgc cctgctgtgc
     4141 gatttctacc ggatgcctag acaggtgttc aatgctcaga agaaggccca gagctccatc
     4201 aactccggag gatctagcgg aggctcctct ggctctgaga cacctggcac aagcgagagc
     4261 gcaacacctg aaagcagcgg gggcagcagc ggggggtcag agacaaacgg cgaaaccggg
     4321 gagatcgtgt gggataaggg ccgggatttt gccaccgtgc ggaaagtgct gagcatgccc
     4381 caagtgaata tcgtgaaaaa gaccgaggtg cagacaggcg gcttcagcaa agagtctatc
     4441 ctgcccaaga ggaacagcga taagctgatc gccagaaaga aggactggga ccctaagaag
     4501 tacggcggct tcgacagccc caccgtggcc tattctgtgc tggtggtggc caaagtggaa
     4561 aagggcaagt ccaagaaact gaagagtgtg aaagagctgc tggggatcac catcatggaa
     4621 agaagcagct tcgagaagaa tcccatcgac tttctggaag ccaagggcta caaagaagtg
     4681 aaaaaggacc tgatcatcaa gctgcctaag tactccctgt tcgagctgga aaacggccgg
     4741 aagagaatgc tggcctctgc cggcgaactg cagaagggaa acgaactggc cctgccctcc
     4801 aaatatgtga acttcctgta cctggccagc cactatgaga agctgaaggg ctcccccgag
     4861 gataatgagc agaaacagct gtttgtggaa cagcacaagc actacctgga cgagatcatc
     4921 gagcagatca gcgagttctc caagagagtg atcctggccg acgctaatct ggacaaagtg
     4981 ctgtccgcct acaacaagca ccgggataag cccatcagag agcaggccga gaatatcatc
     5041 cacctgttta ccctgaccaa tctgggagcc cctgccgcct tcaagtactt tgacaccacc
     5101 atcgaccgga agaggtacac cagcaccaaa gaggtgctgg acgccaccct gatccaccag
     5161 agcatcaccg gcctgtacga gacacggatc gacctgtctc agctgggagg tgactctggc
     5221 ggctcaaaaa gaaccgccga cggcagcgaa ttcgagccca agaagaagag gaaagtcgga
     5281 agcggagcta ctaacttcag cctgctgaag caggctggag acgtggagga gaaccctgga
     5341 cctatggtga gcaagggcga ggagctgttc accggggtgg tgcccatcct ggtcgagctg
     5401 gacggcgacg taaacggcca caagttcagc gtgtccggcg agggcgaggg cgatgccacc
     5461 tacggcaagc tgaccctgaa gttcatctgc accaccggca agctgcccgt gccctggccc
     5521 accctcgtga ccaccctgac ctatggagtg cagtgcttca gccgctaccc cgaccacatg
     5581 aagcagcacg acttcttcaa gtccgccatg cccgaaggct acgtccagga gcgcaccatc
     5641 ttcttcaagg acgacggcaa ctacaagacc cgcgccgagg tgaagttcga gggcgacacc
     5701 ctggtgaacc gcatcgagct gaagggcatc gacttcaagg aggacggcaa catcctgggg
     5761 cacaagctgg agtacaacta caacagccac aacgtctata tcatggccga caagcagaag
     5821 aacggcatca aggtgaactt caagatccgc cacaacatcg aggacggcag cgtgcagctc
     5881 gccgaccact accagcagaa cacccccatc ggcgacggcc ccgtgctgct gcccgacaac
     5941 cactacctga gcacccagtc cgccctgagc aaagacccca acgagaagcg cgatcacatg
     6001 gtcctgctgg agttcgtgac cgccgccggg atcactctcg gcatggacga gctgtacaag
     6061 tctggtggtt ctcccaagaa gaagaggaaa gtctaaccgg tcatcatcac catcaccatt
     6121 gagtttaaac ccgctgatca gcctcgactg tgccttctag ttgccagcca tctgttgttt
     6181 gcccctcccc cgtgccttcc ttgaccctgg aaggtgccac tcccactgtc ctttcctaat
     6241 aaaatgagga aattgcatcg cattgtctga gtaggtgtca ttctattctg gggggtgggg
     6301 tggggcagga cagcaagggg gaggattggg aagacaatag caggcatgct ggggatgcgg
     6361 tgggctctat ggcttctgag gcggaaagaa ccagctgggg ctcgataccg tcgacctcta
     6421 gctagagctt ggcgtaatca tggtcatagc tgtttcctgt gtgaaattgt tatccgctca
     6481 caattccaca caacatacga gccggaagca taaagtgtaa agcctagggt gcctaatgag
     6541 tgagctaact cacattaatt gcgttgcgct cactgcccgc tttccagtcg ggaaacctgt
     6601 cgtgccagct gcattaatga atcggccaac gcgcggggag aggcggtttg cgtattgggc
     6661 gctcttccgc ttcctcgctc actgactcgc tgcgctcggt cgttcggctg cggcgagcgg
     6721 tatcagctca ctcaaaggcg gtaatacggt tatccacaga atcaggggat aacgcaggaa
     6781 agaacatgtg agcaaaaggc cagcaaaagg ccaggaaccg taaaaaggcc gcgttgctgg
     6841 cgtttttcca taggctccgc ccccctgacg agcatcacaa aaatcgacgc tcaagtcaga
     6901 ggtggcgaaa cccgacagga ctataaagat accaggcgtt tccccctgga agctccctcg
     6961 tgcgctctcc tgttccgacc ctgccgctta ccggatacct gtccgccttt ctcccttcgg
     7021 gaagcgtggc gctttctcat agctcacgct gtaggtatct cagttcggtg taggtcgttc
     7081 gctccaagct gggctgtgtg cacgaacccc ccgttcagcc cgaccgctgc gccttatccg
     7141 gtaactatcg tcttgagtcc aacccggtaa gacacgactt atcgccactg gcagcagcca
     7201 ctggtaacag gattagcaga gcgaggtatg taggcggtgc tacagagttc ttgaagtggt
     7261 ggcctaacta cggctacact agaagaacag tatttggtat ctgcgctctg ctgaagccag
     7321 ttaccttcgg aaaaagagtt ggtagctctt gatccggcaa acaaaccacc gctggtagcg
     7381 gtggtttttt tgtttgcaag cagcagatta cgcgcagaaa aaaaggatct caagaagatc
     7441 ctttgatctt ttctacgggg tctgacactc agtggaacga aaactcacgt taagggattt
     7501 tggtcatgag attatcaaaa aggatcttca cctagatcct tttaaattaa aaatgaagtt
     7561 ttaaatcaat ctaaagtata tatgagtaaa cttggtctga cagttaccaa tgcttaatca
     7621 gtgaggcacc tatctcagcg atctgtctat ttcgttcatc catagttgcc tgactccccg
     7681 tcgtgtagat aactacgata cgggagggct taccatctgg ccccagtgct gcaatgatac
     7741 cgcgagaccc acgctcaccg gctccagatt tatcagcaat aaaccagcca gccggaaggg
     7801 ccgagcgcag aagtggtcct gcaactttat ccgcctccat ccagtctatt aattgttgcc
     7861 gggaagctag agtaagtagt tcgccagtta atagtttgcg caacgttgtt gccattgcta
     7921 caggcatcgt ggtgtcacgc tcgtcgtttg gtatggcttc attcagctcc ggttcccaac
     7981 gatcaaggcg agttacatga tcccccatgt tgtgcaaaaa agcggttagc tccttcggtc
     8041 ctccgatcgt tgtcagaagt aagttggccg cagtgttatc actcatggtt atggcagcac
     8101 tgcataattc tcttactgtc atgccatccg taagatgctt ttctgtgact ggtgagtact
     8161 caaccaagtc attctgagaa tagtgtatgc ggcgaccgag ttgctcttgc ccggcgtcaa
     8221 tacgggataa taccgcgcca catagcagaa ctttaaaagt gctcatcatt ggaaaacgtt
     8281 cttcggggcg aaaactctca aggatcttac cgctgttgag atccagttcg atgtaaccca
     8341 ctcgtgcacc caactgatct tcagcatctt ttactttcac cagcgtttct gggtgagcaa
     8401 aaacaggaag gcaaaatgcc gcaaaaaagg gaataagggc gacacggaaa tgttgaatac
     8461 tcatactctt cctttttcaa tattattgaa gcatttatca gggttattgt ctcatgagcg
     8521 gatacatatt tgaatgtatt tagaaaaata aacaaatagg ggttccgcgc acatttcccc
     8581 gaaaagtgcc acctgacgtc gacggatcgg gagatcgatc tcccgatccc ctagggtcga
     8641 ctctcagtac aatctgctct gatgccgcat agttaagcca gtatctgctc cctgcttgtg
     8701 tgttggaggt cgctgagtag tgcgcgagca aaatttaagc tacaacaagg caaggcttga
     8761 ccgacaattg catgaagaat ctgcttaggg ttaggcgttt tgcgctgctt cgcgatgtac
     8821 gggccagata tacgcgttga cattgattat tgactagtta ttaatagtaa tcaattacgg
     8881 ggtcattagt tcatagccca tatatggagt tccgcgttac ataacttacg gtaaatggcc
     8941 cgcctggctg accgcccaac gacccccgcc cattgacgtc aataatgacg tatgttccca
     9001 tagtaacgcc aatagggact ttccattgac gtcaatgggt ggagtattta cggtaaactg
     9061 cccacttggc agtacatcaa gtgtatc
//
