LOCUS       sequence-410849-        9009 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  CMV and T7 promoter expression plasmid for human codon optimized 
            ABE8e A-to-G base editor with iSpyMac(D10A) and P2A-EGFP.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9009)
  AUTHORS   Alves CRR, Ha LL, Yaworski R, Sutton ER, Lazzarotto CR, Christie KA,
            Reilly A, Beauvais A, Doll RM, de la Cruz D, Maguire CA, Swoboda KJ,
            Tsai SQ, Kothary R, Kleinstiver BP
  TITLE     Optimization of base editors for the functional correction of SMN2 
            as a treatment for spinal muscular atrophy.
  JOURNAL   Nat Biomed Eng. 2023 Dec 6. doi: 10.1038/s41551-023-01132-z.
  PUBMED    38057426
REFERENCE   2  (bases 1 to 9009)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat Biomed 
            Eng. 2023 Dec 6. doi: 10.1038/s41551-023-01132-z."
COMMENT     Sequence Label: pCMV-T7-BPNLS-ABE8e-iSpyMac-BPNLS-P2A-EGFP (LL...
FEATURES             Location/Qualifiers
     source          1..9009
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        132..335
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     286..306
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     377..396
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        377..395
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             445..465
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             1060..2775
                     /codon_start=1
                     /product="N-terminal portion of Streptococcus pyogenes Cas9
                     (Zetsche et al., 2015)"
                     /label=Cas9(N)
                     /translation="DKKYSIGLAIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKKN
                     LIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEESF
                     LVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIKF
                     RGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRKLE
                     NLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQI
                     GDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVRQ
                     QLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLKREDLLR
                     KQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARGN
                     SRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEYF
                     TVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIE"
     CDS             5179..5199
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             5209..5265
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
     CDS             join(5266..5268,5269..5271,5272..5982)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             join(5266..5268,5269..5271,5272..5982)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(5311..5332)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(5572..5591)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     5919..5940
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     CDS             5995..6015
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             6024..6041
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(6064..6081)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    6070..6294
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     primer_bind     complement(6365..6381)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(6365..6381)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(6378..6400)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    6389..6405
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(6413..6419,6420..6437,6438..6443))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    6458..6479
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(6596..6613)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(6767..7355)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(6847..6866)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(7526..8317,8318..8386))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     8149..8168
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(8387..8491)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(8570..8589)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        join(8761..9009,1..131)
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
ORIGIN
        1 atatgccaag tacgccccct attgacgtca atgacggtaa atggcccgcc tggcattatg
       61 cccagtacat gaccttatgg gactttccta cttggcagta catctacgta ttagtcatcg
      121 ctattaccat ggtgatgcgg ttttggcagt acatcaatgg gcgtggatag cggtttgact
      181 cacggggatt tccaagtctc caccccattg acgtcaatgg gagtttgttt tggcaccaaa
      241 atcaacggga ctttccaaaa tgtcgtaaca actccgcccc attgacgcaa atgggcggta
      301 ggcgtgtacg gtgggaggtc tatataagca gagctggttt agtgaaccgt cagatccgct
      361 agagatccgc ggccgctaat acgactcact atagggagag ccgccaccat gaaacggaca
      421 gccgacggaa gcgagttcga gtcaccaaag aagaagcgga aagtctctga ggtggagttt
      481 tcccacgagt actggatgag acatgccctg accctggcca agagggcacg cgatgagagg
      541 gaggtgcctg tgggagccgt gctggtgctg aacaatagag tgatcggcga gggctggaac
      601 agagccatcg gcctgcacga cccaacagcc catgccgaaa ttatggccct gagacagggc
      661 ggcctggtca tgcagaacta cagactgatt gacgccaccc tgtacgtgac attcgagcct
      721 tgcgtgatgt gcgccggcgc catgatccac tctaggatcg gccgcgtggt gtttggcgtg
      781 aggaacagca aaagaggcgc cgcaggctcc ctgatgaacg tgctgaacta ccccggcatg
      841 aatcaccgcg tcgaaattac cgagggaatc ctggcagatg aatgtgccgc cctgctgtgc
      901 gatttctacc ggatgcctag acaggtgttc aatgctcaga agaaggccca gagctccatc
      961 aactccggag gatctagcgg aggctcctct ggctctgaga cacctggcac aagcgagagc
     1021 gcaacacctg aaagcagcgg gggcagcagc ggggggtcag acaagaagta cagcatcggc
     1081 ctggccatcg gcaccaactc tgtgggctgg gccgtgatca ccgacgagta caaggtgccc
     1141 agcaagaaat tcaaggtgct gggcaacacc gaccggcaca gcatcaagaa gaacctgatc
     1201 ggagccctgc tgttcgacag cggcgaaaca gccgaggcca cccggctgaa gagaaccgcc
     1261 agaagaagat acaccagacg gaagaaccgg atctgctatc tgcaagagat cttcagcaac
     1321 gagatggcca aggtggacga cagcttcttc cacagactgg aagagtcctt cctggtggaa
     1381 gaggataaga agcacgagcg gcaccccatc ttcggcaaca tcgtggacga ggtggcctac
     1441 cacgagaagt accccaccat ctaccacctg agaaagaaac tggtggacag caccgacaag
     1501 gccgacctgc ggctgatcta tctggccctg gcccacatga tcaagttccg gggccacttc
     1561 ctgatcgagg gcgacctgaa ccccgacaac agcgacgtgg acaagctgtt catccagctg
     1621 gtgcagacct acaaccagct gttcgaggaa aaccccatca acgccagcgg cgtggacgcc
     1681 aaggccatcc tgtctgccag actgagcaag agcagaaagc tggaaaatct gatcgcccag
     1741 ctgcccggcg agaagaagaa tggcctgttc ggaaacctga ttgccctgag cctgggcctg
     1801 acccccaact tcaagagcaa cttcgacctg gccgaggatg ccaaactgca gctgagcaag
     1861 gacacctacg acgacgacct ggacaacctg ctggcccaga tcggcgacca gtacgccgac
     1921 ctgtttctgg ccgccaagaa cctgtccgac gccatcctgc tgagcgacat cctgagagtg
     1981 aacaccgaga tcaccaaggc ccccctgagc gcctctatga tcaagagata cgacgagcac
     2041 caccaggacc tgaccctgct gaaagctctc gtgcggcagc agctgcctga gaagtacaaa
     2101 gagattttct tcgaccagag caagaacggc tacgccggct acattgacgg cggagccagc
     2161 caggaagagt tctacaagtt catcaagccc atcctggaaa agatggacgg caccgaggaa
     2221 ctgctcgtga agctgaagag agaggacctg ctgcggaagc agcggacctt cgacaacggc
     2281 agcatccccc accagatcca cctgggagag ctgcacgcca ttctgcggcg gcaggaagat
     2341 ttttacccat tcctgaagga caaccgggaa aagatcgaga agatcctgac cttccgcatc
     2401 ccctactacg tgggccctct ggccagggga aacagcagat tcgcctggat gaccagaaag
     2461 agcgaggaaa ccatcacccc ctggaacttc gaggaagtgg tggacaaggg cgcttccgcc
     2521 cagagcttca tcgagcggat gaccaacttc gataagaacc tgcccaacga gaaggtgctg
     2581 cccaagcaca gcctgctgta cgagtacttc accgtgtata acgagctgac caaagtgaaa
     2641 tacgtgaccg agggaatgag aaagcccgcc ttcctgagcg gcgagcagaa aaaggccatc
     2701 gtggacctgc tgttcaagac caaccggaaa gtgaccgtga agcagctgaa agaggactac
     2761 ttcaagaaaa tcgagtgctt cgactccgtg gaaatctccg gcgtggaaga tcggttcaac
     2821 gcctccctgg gcacatacca cgatctgctg aaaattatca aggacaagga cttcctggac
     2881 aatgaggaaa acgaggacat tctggaagat atcgtgctga ccctgacact gtttgaggac
     2941 agagagatga tcgaggaacg gctgaaaacc tatgcccacc tgttcgacga caaagtgatg
     3001 aagcagctga agcggcggag atacaccggc tggggcaggc tgagccggaa gctgatcaac
     3061 ggcatccggg acaagcagtc cggcaagaca atcctggatt tcctgaagtc cgacggcttc
     3121 gccaacagaa acttcatgca gctgatccac gacgacagcc tgacctttaa agaggacatc
     3181 cagaaagccc aggtgtccgg ccagggcgat agcctgcacg agcacattgc caatctggcc
     3241 ggcagccccg ccattaagaa gggcatcctg cagacagtga aggtggtgga cgagctcgtg
     3301 aaagtgatgg gccggcacaa gcccgagaac atcgtgatcg aaatggccag agagaaccag
     3361 accacccaga agggacagaa gaacagccgc gagagaatga agcggatcga agagggcatc
     3421 aaagagctgg gcagccagat cctgaaagaa caccccgtgg aaaacaccca gctgcagaac
     3481 gagaagctgt acctgtacta cctgcagaat gggcgggata tgtacgtgga ccaggaactg
     3541 gacatcaacc ggctgtccga ctacgatgtg gaccatatcg tgcctcagag ctttctgaag
     3601 gacgactcca tcgacaacaa ggtgctgacc agaagcgaca agaaccgggg caagagcgac
     3661 aacgtgccct ccgaagaggt cgtgaagaag atgaagaact actggcggca gctgctgaac
     3721 gccaagctga ttacccagag aaagttcgac aatctgacca aggccgagag aggcggcctg
     3781 agcgaactgg ataaggccgg cttcatcaag agacagctgg tggaaacccg gcagatcaca
     3841 aagcacgtgg cacagatcct ggactcccgg atgaacacta agtacgacga gaatgacaag
     3901 ctgatccggg aagtgaaagt gatcaccctg aagtccaagc tggtgtccga tttccggaag
     3961 gatttccagt tttacaaagt gcgcgagatc aacaactacc accacgccca cgacgcctac
     4021 ctgaacgccg tcgtgggaac cgccctgatc aaaaagtacc ctaagctgga aagcgagttc
     4081 gtgtacggcg actacaaggt gtacgacgtg cggaagatga tcgccaagag cgagcaggaa
     4141 atcggcaagg ctaccgccaa gtacttcttc tacagcaaca tcatgaactt tttcaagacc
     4201 gagattaccc tggccaacgg cgagatccgg aagcggcctc tgatcgagac aaacggcgaa
     4261 accggggaga tcgtgtggga taagggccgg gattttgcca ccgtgcggaa agtgctgagc
     4321 atgccccaag tgaatatcgt gaaaaagacc gagatccaga ccgtgggaca gaatggaggc
     4381 ctgttcgacg acaaccccaa gagccccctg gaggtgaccc ccagcaaact ggtgcccctg
     4441 aagaaagagc tgaaccccaa gaaatacggc ggctaccaga agcccaccac cgcctacccc
     4501 gtgctgctga tcaccgacac caagcagctg atccccatca gcgtgatgaa caagaaacag
     4561 ttcgagcaga accccgtgaa gttcctgcgg gaccggggct accagcaggt gggcaagaac
     4621 gacttcatca agctgcccaa gtacaccctg gtggacatcg gcgacggcat caagcggctg
     4681 tgggccagca gcaaggagat ccacaagggc aaccagctgg tggtgagcaa gaagagccag
     4741 atcctgctgt accacgccca ccacctggac agcgacctga gcaacgacta cctgcagaac
     4801 cacaaccagc agttcgacgt gctgttcaac gagatcatca gcttcagcaa aaagtgcaag
     4861 ctgggcaagg agcacatcca gaagatcgag aacgtgtaca gcaacaagaa gaacagcgcc
     4921 agcatcgagg agctggccga gagctttatc aaactgctcg gcttcaccca gctgggcgcc
     4981 accagcccct tcaacttcct gggcgtgaag ctgaaccaga agcagtacaa gggcaagaag
     5041 gactacatcc tgccctgcac cgagggcacc ctgatcagac agagcatcac cggactgtac
     5101 gagaccagag tggatctgag caagatcggc gaggattctg gcggctcaaa aagaaccgcc
     5161 gacggcagcg aattcgagcc caagaagaag aggaaagtcg gaagcggagc tactaacttc
     5221 agcctgctga agcaggctgg agacgtggag gagaaccctg gacctatggt gagcaagggc
     5281 gaggagctgt tcaccggggt ggtgcccatc ctggtcgagc tggacggcga cgtaaacggc
     5341 cacaagttca gcgtgtccgg cgagggcgag ggcgatgcca cctacggcaa gctgaccctg
     5401 aagttcatct gcaccaccgg caagctgccc gtgccctggc ccaccctcgt gaccaccctg
     5461 acctatggag tgcagtgctt cagccgctac cccgaccaca tgaagcagca cgacttcttc
     5521 aagtccgcca tgcccgaagg ctacgtccag gagcgcacca tcttcttcaa ggacgacggc
     5581 aactacaaga cccgcgccga ggtgaagttc gagggcgaca ccctggtgaa ccgcatcgag
     5641 ctgaagggca tcgacttcaa ggaggacggc aacatcctgg ggcacaagct ggagtacaac
     5701 tacaacagcc acaacgtcta tatcatggcc gacaagcaga agaacggcat caaggtgaac
     5761 ttcaagatcc gccacaacat cgaggacggc agcgtgcagc tcgccgacca ctaccagcag
     5821 aacaccccca tcggcgacgg ccccgtgctg ctgcccgaca accactacct gagcacccag
     5881 tccgccctga gcaaagaccc caacgagaag cgcgatcaca tggtcctgct ggagttcgtg
     5941 accgccgccg ggatcactct cggcatggac gagctgtaca agtctggtgg ttctcccaag
     6001 aagaagagga aagtctaacc ggtcatcatc accatcacca ttgagtttaa acccgctgat
     6061 cagcctcgac tgtgccttct agttgccagc catctgttgt ttgcccctcc cccgtgcctt
     6121 ccttgaccct ggaaggtgcc actcccactg tcctttccta ataaaatgag gaaattgcat
     6181 cgcattgtct gagtaggtgt cattctattc tggggggtgg ggtggggcag gacagcaagg
     6241 gggaggattg ggaagacaat agcaggcatg ctggggatgc ggtgggctct atggcttctg
     6301 aggcggaaag aaccagctgg ggctcgatac cgtcgacctc tagctagagc ttggcgtaat
     6361 catggtcata gctgtttcct gtgtgaaatt gttatccgct cacaattcca cacaacatac
     6421 gagccggaag cataaagtgt aaagcctagg gtgcctaatg agtgagctaa ctcacattaa
     6481 ttgcgttgcg ctcactgccc gctttccagt cgggaaacct gtcgtgccag ctgcattaat
     6541 gaatcggcca acgcgcgggg agaggcggtt tgcgtattgg gcgctcttcc gcttcctcgc
     6601 tcactgactc gctgcgctcg gtcgttcggc tgcggcgagc ggtatcagct cactcaaagg
     6661 cggtaatacg gttatccaca gaatcagggg ataacgcagg aaagaacatg tgagcaaaag
     6721 gccagcaaaa ggccaggaac cgtaaaaagg ccgcgttgct ggcgtttttc cataggctcc
     6781 gcccccctga cgagcatcac aaaaatcgac gctcaagtca gaggtggcga aacccgacag
     6841 gactataaag ataccaggcg tttccccctg gaagctccct cgtgcgctct cctgttccga
     6901 ccctgccgct taccggatac ctgtccgcct ttctcccttc gggaagcgtg gcgctttctc
     6961 atagctcacg ctgtaggtat ctcagttcgg tgtaggtcgt tcgctccaag ctgggctgtg
     7021 tgcacgaacc ccccgttcag cccgaccgct gcgccttatc cggtaactat cgtcttgagt
     7081 ccaacccggt aagacacgac ttatcgccac tggcagcagc cactggtaac aggattagca
     7141 gagcgaggta tgtaggcggt gctacagagt tcttgaagtg gtggcctaac tacggctaca
     7201 ctagaagaac agtatttggt atctgcgctc tgctgaagcc agttaccttc ggaaaaagag
     7261 ttggtagctc ttgatccggc aaacaaacca ccgctggtag cggtggtttt tttgtttgca
     7321 agcagcagat tacgcgcaga aaaaaaggat ctcaagaaga tcctttgatc ttttctacgg
     7381 ggtctgacac tcagtggaac gaaaactcac gttaagggat tttggtcatg agattatcaa
     7441 aaaggatctt cacctagatc cttttaaatt aaaaatgaag ttttaaatca atctaaagta
     7501 tatatgagta aacttggtct gacagttacc aatgcttaat cagtgaggca cctatctcag
     7561 cgatctgtct atttcgttca tccatagttg cctgactccc cgtcgtgtag ataactacga
     7621 tacgggaggg cttaccatct ggccccagtg ctgcaatgat accgcgagac ccacgctcac
     7681 cggctccaga tttatcagca ataaaccagc cagccggaag ggccgagcgc agaagtggtc
     7741 ctgcaacttt atccgcctcc atccagtcta ttaattgttg ccgggaagct agagtaagta
     7801 gttcgccagt taatagtttg cgcaacgttg ttgccattgc tacaggcatc gtggtgtcac
     7861 gctcgtcgtt tggtatggct tcattcagct ccggttccca acgatcaagg cgagttacat
     7921 gatcccccat gttgtgcaaa aaagcggtta gctccttcgg tcctccgatc gttgtcagaa
     7981 gtaagttggc cgcagtgtta tcactcatgg ttatggcagc actgcataat tctcttactg
     8041 tcatgccatc cgtaagatgc ttttctgtga ctggtgagta ctcaaccaag tcattctgag
     8101 aatagtgtat gcggcgaccg agttgctctt gcccggcgtc aatacgggat aataccgcgc
     8161 cacatagcag aactttaaaa gtgctcatca ttggaaaacg ttcttcgggg cgaaaactct
     8221 caaggatctt accgctgttg agatccagtt cgatgtaacc cactcgtgca cccaactgat
     8281 cttcagcatc ttttactttc accagcgttt ctgggtgagc aaaaacagga aggcaaaatg
     8341 ccgcaaaaaa gggaataagg gcgacacgga aatgttgaat actcatactc ttcctttttc
     8401 aatattattg aagcatttat cagggttatt gtctcatgag cggatacata tttgaatgta
     8461 tttagaaaaa taaacaaata ggggttccgc gcacatttcc ccgaaaagtg ccacctgacg
     8521 tcgacggatc gggagatcga tctcccgatc ccctagggtc gactctcagt acaatctgct
     8581 ctgatgccgc atagttaagc cagtatctgc tccctgcttg tgtgttggag gtcgctgagt
     8641 agtgcgcgag caaaatttaa gctacaacaa ggcaaggctt gaccgacaat tgcatgaaga
     8701 atctgcttag ggttaggcgt tttgcgctgc ttcgcgatgt acgggccaga tatacgcgtt
     8761 gacattgatt attgactagt tattaatagt aatcaattac ggggtcatta gttcatagcc
     8821 catatatgga gttccgcgtt acataactta cggtaaatgg cccgcctggc tgaccgccca
     8881 acgacccccg cccattgacg tcaataatga cgtatgttcc catagtaacg ccaataggga
     8941 ctttccattg acgtcaatgg gtggagtatt tacggtaaac tgcccacttg gcagtacatc
     9001 aagtgtatc
//
