LOCUS       sequence-410860-        8379 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8379)
  AUTHORS   Hagerstrand D, Tong A, Schumacher SE, Ilic N, Shen RR, Cheung HW, 
            Vazquez F, Shrestha Y, Kim SY, Giacomelli AO, Rosenbluh J, Schinzel 
            AC, Spardy NA, Barbie DA, Mermel CH, Weir BA, Garraway LA, Tamayo P,
            Mesirov JP, Beroukhim R, Hahn WC
  TITLE     Systematic interrogation of 3q26 identifies TLOC1 and SKIL as cancer
            drivers.
  JOURNAL   Cancer Discov. 2013 Jun 13. [Epub ahead of print]
  PUBMED    23764425
REFERENCE   2  (bases 1 to 8379)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cancer 
            Discov. 2013 Jun 13. [Epub ahead of print]"
COMMENT     Sequence Label: pBabe_puro_DEST_Flag_PLD1
FEATURES             Location/Qualifiers
     source          1..8379
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     LTR             8..477
                     /note="long terminal repeat from Moloney murine leukemia 
                     virus"
     misc_feature    542..741
                     /label=MMLV Psi
                     /note="packaging signal of Moloney murine leukemia virus 
                     (MMLV)"
     CDS             942..1358
                     /label=gag (truncated)
                     /note="truncated Moloney murine leukemia virus (MMLV) gag 
                     gene lacking the start codon"
     primer_bind     1288..1310
                     /label=pLXSN 5'
                     /note="Murine stem cell virus, forward primer. Also called 
                     MSCV"
     primer_bind     1326..1342
                     /label=pBABE 5'
                     /note="Psi packaging signal, forward primer for pBABE 
                     vectors"
     CDS             1396..1419
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     protein_bind    1426..1450
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(4673..4697)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     primer_bind     complement(4709..4729)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        4714..5043
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      4894..5029
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             5053..5652
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(5053..5072)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     5549..5569
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     LTR             5873..6342
                     /note="long terminal repeat from Moloney murine leukemia 
                     virus"
     primer_bind     complement(6472..6489)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(6643..7231)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(6723..6742)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(7391..8182,8183..8251))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     8014..8033
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(8252..8356)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 ctgcagcctg aatatgggcc aaacaggata tctgtggtaa gcagttcctg ccccggctca
       61 gggccaagaa cagatggaac agctgaatat gggccaaaca ggatatctgt ggtaagcagt
      121 tcctgccccg gctcagggcc aagaacagat ggtccccaga tgcggtccag ccctcagcag
      181 tttctagaga accatcagat gtttccaggg tgccccaagg acctgaaatg accctgtgcc
      241 ttatttgaac taaccaatca gttcgcttct cgcttctgtt cgcgcgcttc tgctccccga
      301 gctcaataaa agagcccaca acccctcact cggggcgcca gtcctccgat tgactgagtc
      361 gcccgggtac ccgtgtatcc aataaaccct cttgcagttg catccgactt gtggtctcgc
      421 tgttccttgg gagggtctcc tctgagtgat tgactacccg tcagcggggg tctttcattt
      481 gggggctcgt ccgggatcgg gagacccctg cccagggacc accgacccac caccgggagg
      541 caagctggcc agcaacttat ctgtgtctgt ccgattgtct agtgtctatg actgatttta
      601 tgcgcctgcg tcggtactag ttagctaact agctctgtat ctggcggacc cgtggtggaa
      661 ctgacgagtt ctgaacaccc ggccgcaacc ctgggagacg tcccagggac tttgggggcc
      721 gtttttgtgg cccgacctga ggaagggagt cgatgtggaa tccgaccccg tcaggatatg
      781 tggttctggt aggagacgag aacctaaaac agttcccgcc tccgtctgaa tttttgcttt
      841 cggtttggaa ccgaagccgc gcgtcttgtc tgctgcagcg ctgcagcatc gttctgtgtt
      901 gtctctgtct gactgtgttt ctgtatttgt ctgaaaatta gggccagact gttaccactc
      961 ccttaagttt gaccttaggt cactggaaag atgtcgagcg gatcgctcac aaccagtcgg
     1021 tagatgtcaa gaagagacgt tgggttacct tctgctctgc agaatggcca acctttaacg
     1081 tcggatggcc gcgagacggc acctttaacc gagacctcat cacccaggtt aagatcaagg
     1141 tcttttcacc tggcccgcat ggacacccag accaggtccc ctacatcgtg acctgggaag
     1201 ccttggcttt tgacccccct ccctgggtca agccctttgt acaccctaag cctccgcctc
     1261 ctcttcctcc atccgccccg tctctccccc ttgaacctcc tcgttcgacc ccgcctcgat
     1321 cctcccttta tccagccctc actccttctc taggcgccgg ccggatccca gtgtggtggt
     1381 acgtaggaat tcatggacta caaagacgat gacgacaagc aattgacaag tttgtacaaa
     1441 aaagttggca tgtcactgaa aaacgagcca cgggtaaata cctctgcact gcagaaaatt
     1501 gctgctgaca tgagtaatat catagaaaat ctggacacgc gggaactcca ctttgaggga
     1561 gaggaggtag actacgacgt gtctcccagc gatcccaaga tacaagaagt gtatatccct
     1621 ttctctgcta tttataacac tcaaggattt aaggagccta atatacagac gtatctctcc
     1681 ggctgtccaa taaaagcaca agttctggaa gtggaacgct tcacatctac aacaagggta
     1741 ccaagtatta atctttacac tattgaatta acacatgggg aatttaaatg gcaagttaag
     1801 aggaaattca agcattttca agaatttcac agagagctgc tcaagtacaa agcctttatc
     1861 cgcatcccca ttcccactag aagacacacg tttaggaggc aaaacgtcag agaggagcct
     1921 cgagagatgc ccagtttgcc ccgttcatct gaaaacatga taagagaaga acaattcctt
     1981 ggtagaagaa aacaactgga agattacttg acaaagatac taaaaatgcc catgtataga
     2041 aactatcatg ccacaacaga gtttcttgat ataagccagc tgtctttcat ccatgatttg
     2101 ggaccaaagg gcatagaagg tatgataatg aaaagatctg gaggacacag aataccaggc
     2161 ttgaattgct gtggtcaggg aagagcctgc tacagatggt caaaaagatg gttaatagtg
     2221 aaagattcct ttttattgta tatgaaacca gacagcggtg ccattgcctt cgtcctgctg
     2281 gtagacaaag aattcaaaat taaggtgggg aagaaggaga cagaaacgaa atatggaatc
     2341 cgaattgata atctttcaag gacacttatt ttaaaatgca acagctatag acatgctcgg
     2401 tggtggggag gggctataga agaattcatc cagaaacatg gcaccaactt tctcaaagat
     2461 catcgatttg ggtcatatgc tgctatccaa gagaatgctt tagctaaatg gtatgttaat
     2521 gccaaaggat attttgaaga tgtggcaaat gcaatggaag aggcaaatga agagattttt
     2581 atcacagact ggtggctgag tccagaaatc ttcctgaaac gcccagtggt tgagggaaat
     2641 cgttggaggt tggactgcat tcttaaacga aaagcacaac aaggagtgag gatcttcata
     2701 atgctctaca aagaggtgga actcgctctt ggcatcaata gtgaatacac caagaggact
     2761 ttgatgcgtc tacatcccaa cataaaggtg atgagacacc cggatcatgt gtcatccacc
     2821 gtctatttgt gggctcacca tgagaagctt gtcatcattg accaatcggt ggcctttgtg
     2881 ggagggattg acctggccta tggaaggtgg gacgacaatg agcacagact cacagacgtg
     2941 ggcagtgtga agcgggtcac ttcaggaccg tctctgggtt ccctcccacc tgccgcaatg
     3001 gagtctatgg aatccttaag actcaaagat aaaaatgagc ctgttcaaaa cctacccatc
     3061 cagaagagta ttgatgatgt ggattcaaaa ctgaaaggaa taggaaagcc aagaaagttc
     3121 tccaaattta gtctctacaa gcagctccac aggcaccacc tgcacgacgc agatagcatc
     3181 agcagcattg acagcacctc cagttatttt aatcactata gaagtcatca caatttaatc
     3241 catggtttaa aaccccactt caaactcttt cacccgtcca gtgagtctga gcaaggactc
     3301 actagacctc atgctgatac cgggtccatc cgtagtttac agacaggtgt gggagagctg
     3361 catggggaaa ccagattctg gcatggaaag gactactgca atttcgtctt caaagactgg
     3421 gttcaacttg ataaaccttt tgctgatttc attgacaggt actccacgcc ccggatgccc
     3481 tggcatgaca ttgcctctgc agtccacggg aaggcggctc gtgatgtggc acgtcacttc
     3541 atccagcgct ggaacttcac aaaaattatg aaatcaaaat atcggtccct ttcttatcct
     3601 tttctgcttc caaagtctca aacaacagcc catgagttga gatatcaagt gcctgggtct
     3661 gtccatgcta acgtacagtt gctccgctct gctgctgatt ggtctgctgg tataaagtac
     3721 catgaagagt ccatccacgc cgcttacgtc catgtgatag agaacagcag gcactatatc
     3781 tatatcgaaa accagttttt cataagctgt gctgatgaca aagttgtgtt caacaagata
     3841 ggcgatgcca ttgcccagag gatcctgaaa gctcacaggg aaaaccagaa ataccgggta
     3901 tatgtcgtga taccacttct gccagggttc gaaggagaca tttcaaccgg cggaggaaat
     3961 gctctacagg caatcatgca cttcaactac agaaccatgt gcagaggaga aaattccatc
     4021 cttggacagt taaaagcaga gcttggtaat cagtggataa attacatatc attctgtggt
     4081 cttagaacac atgcagagct cgaaggaaac ctagtaactg agcttatcta tgtccacagc
     4141 aagttgttaa ttgctgatga taacactgtt attattggct ctgccaacat aaatgaccgc
     4201 agcatgctgg gaaagcgtga cagtgaaatg gctgtcattg tgcaagatac agagactgtt
     4261 ccttcagtaa tggatggaaa agagtaccaa gctggccggt ttgcccgagg acttcggcta
     4321 cagtgcttta gggttgtcct tggctatctt gatgacccaa gtgaggacat tcaggatcca
     4381 gtgagtgaca aattcttcaa ggaggtgtgg gtttcaacag cagctcgaaa tgctacaatt
     4441 tatgacaagg ttttccggtg ccttcccaat gatgaagtac acaatttaat tcagctgaga
     4501 gactttataa acaagcccgt attagctaag gaagatccca ttcgagctga ggaggaactg
     4561 aagaagatcc gtggattttt ggtgcaattc cccttttatt tcttgtctga agaaagccta
     4621 ctgccttctg ttgggaccaa agaggccata gtgcccatgg aggtttggac ttacccaact
     4681 ttcttgtaca aagtggtctc gaccctgtgg aatgtgtgtc agttagggtg tggaaagtcc
     4741 ccaggctccc cagcaggcag aagtatgcaa agcatgcatc tcaattagtc agcaaccagg
     4801 tgtggaaagt ccccaggctc cccagcaggc agaagtatgc aaagcatgca tctcaattag
     4861 tcagcaacca tagtcccgcc cctaactccg cccatcccgc ccctaactcc gcccagttcc
     4921 gcccattctc cgccccatgg ctgactaatt ttttttattt atgcagaggc cgaggccgcc
     4981 tcggcctctg agctattcca gaagtagtga ggaggctttt ttggaggcct aggcttttgc
     5041 aaaaagctta ccatgaccga gtacaagccc acggtgcgcc tcgccacccg cgacgacgtc
     5101 cccagggccg tacgcaccct cgccgccgcg ttcgccgact accccgccac gcgccacacc
     5161 gtcgatccgg accgccacat cgagcgggtc accgagctgc aagaactctt cctcacgcgc
     5221 gtcgggctcg acatcggcaa ggtgtgggtc gcggacgacg gcgccgcggt ggcggtctgg
     5281 accacgccgg agagcgtcga agcgggggcg gtgttcgccg agatcggccc gcgcatggcc
     5341 gagttgagcg gttcccggct ggccgcgcag caacagatgg aaggcctcct ggcgccgcac
     5401 cggcccaagg agcccgcgtg gttcctggcc accgtcggcg tctcgcccga ccaccagggc
     5461 aagggtctgg gcagcgccgt cgtgctcccc ggagtggagg cggccgagcg cgccggggtg
     5521 cccgccttcc tggagacctc cgcgccccgc aacctcccct tctacgagcg gctcggcttc
     5581 accgtcaccg ccgacgtcga ggtgcccgaa ggaccgcgca cctggtgcat gacccgcaag
     5641 cccggtgcct gacgcccgcc ccacgacccg cagcgcccga ccgaaaggag cgcacgaccc
     5701 catgcatcga taaaataaaa gattttattt agtctccaga aaaagggggg aatgaaagac
     5761 cccacctgta ggtttggcaa gctagcttaa gtaacgccat tttgcaaggc atggaaaaat
     5821 acataactga gaatagagaa gttcagatca aggtcaggaa cagatggaac agctgaatat
     5881 gggccaaaca ggatatctgt ggtaagcagt tcctgccccg gctcagggcc aagaacagat
     5941 ggaacagctg aatatgggcc aaacaggata tctgtggtaa gcagttcctg ccccggctca
     6001 gggccaagaa cagatggtcc ccagatgcgg tccagccctc agcagtttct agagaaccat
     6061 cagatgtttc cagggtgccc caaggacctg aaatgaccct gtgccttatt tgaactaacc
     6121 aatcagttcg cttctcgctt ctgttcgcgc gcttctgctc cccgagctca ataaaagagc
     6181 ccacaacccc tcactcgggg cgccagtcct ccgattgact gagtcgcccg ggtacccgtg
     6241 tatccaataa accctcttgc agttgcatcc gacttgtggt ctcgctgttc cttgggaggg
     6301 tctcctctga gtgattgact acccgtcagc gggggtcttt cacatgcagc atgtatcaaa
     6361 attaatttgg ttttttttct taagtattta cattaaatgg ccatagttgc attaatgaat
     6421 cggccaacgc gcggggagag gcggtttgcg tattgggcgc tcttccgctt cctcgctcac
     6481 tgactcgctg cgctcggtcg ttcggctgcg gcgagcggta tcagctcact caaaggcggt
     6541 aatacggtta tccacagaat caggggataa cgcaggaaag aacatgtgag caaaaggcca
     6601 gcaaaaggcc aggaaccgta aaaaggccgc gttgctggcg tttttccata ggctccgccc
     6661 ccctgacgag catcacaaaa atcgacgctc aagtcagagg tggcgaaacc cgacaggact
     6721 ataaagatac caggcgtttc cccctggaag ctccctcgtg cgctctcctg ttccgaccct
     6781 gccgcttacc ggatacctgt ccgcctttct cccttcggga agcgtggcgc tttctcatag
     6841 ctcacgctgt aggtatctca gttcggtgta ggtcgttcgc tccaagctgg gctgtgtgca
     6901 cgaacccccc gttcagcccg accgctgcgc cttatccggt aactatcgtc ttgagtccaa
     6961 cccggtaaga cacgacttat cgccactggc agcagccact ggtaacagga ttagcagagc
     7021 gaggtatgta ggcggtgcta cagagttctt gaagtggtgg cctaactacg gctacactag
     7081 aagaacagta tttggtatct gcgctctgct gaagccagtt accttcggaa aaagagttgg
     7141 tagctcttga tccggcaaac aaaccaccgc tggtagcggt ggtttttttg tttgcaagca
     7201 gcagattacg cgcagaaaaa aaggatctca agaagatcct ttgatctttt ctacggggtc
     7261 tgacgctcag tggaacgaaa actcacgtta agggattttg gtcatgagat tatcaaaaag
     7321 gatcttcacc tagatccttt tgcggccgca aatcaatcta aagtatatat gagtaaactt
     7381 ggtctgacag ttaccaatgc ttaatcagtg aggcacctat ctcagcgatc tgtctatttc
     7441 gttcatccat agttgcctga ctccccgtcg tgtagataac tacgatacgg gagggcttac
     7501 catctggccc cagtgctgca atgataccgc gagacccacg ctcaccggct ccagatttat
     7561 cagcaataaa ccagccagcc ggaagggccg agcgcagaag tggtcctgca actttatccg
     7621 cctccatcca gtctattaat tgttgccggg aagctagagt aagtagttcg ccagttaata
     7681 gtttgcgcaa cgttgttgcc attgctacag gcatcgtggt gtcacgctcg tcgtttggta
     7741 tggcttcatt cagctccggt tcccaacgat caaggcgagt tacatgatcc cccatgttgt
     7801 gcaaaaaagc ggttagctcc ttcggtcctc cgatcgttgt cagaagtaag ttggccgcag
     7861 tgttatcact catggttatg gcagcactgc ataattctct tactgtcatg ccatccgtaa
     7921 gatgcttttc tgtgactggt gagtactcaa ccaagtcatt ctgagaatag tgtatgcggc
     7981 gaccgagttg ctcttgcccg gcgtcaatac gggataatac cgcgccacat agcagaactt
     8041 taaaagtgct catcattgga aaacgttctt cggggcgaaa actctcaagg atcttaccgc
     8101 tgttgagatc cagttcgatg taacccactc gtgcacccaa ctgatcttca gcatctttta
     8161 ctttcaccag cgtttctggg tgagcaaaaa caggaaggca aaatgccgca aaaaagggaa
     8221 taagggcgac acggaaatgt tgaatactca tactcttcct ttttcaatat tattgaagca
     8281 tttatcaggg ttattgtctc atgagcggat acatatttga atgtatttag aaaaataaac
     8341 aaataggggt tccgcgcaca tttccccgaa aagtgccac
//
