LOCUS       sequence-410861-       11383 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Conditionally-replicating in Pseudomonas plasmid for adenine base 
            editing based on pS44i8GH-2; oriV(pRO1600/ColE1), xylS (cured of 
            BsaI-sites), Pm-->repA, P14b-->msfGFP; Pm--> ABE:SpRY, 
            PEM7-->non-specific sg.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11383)
  AUTHORS   Kozaeva E, Nielsen ZS, Nieto-Dominguez M, Nikel PI
  TITLE     The pAblo.pCasso self-curing vector toolset for unconstrained 
            cytidine and adenine base-editing in Gram-negative bacteria.
  JOURNAL   Nucleic Acids Res. 2024 Jan 5:gkad1236. doi: 10.1093/nar/gkad1236.
  PUBMED    38180826
REFERENCE   2  (bases 1 to 11383)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nucleic 
            Acids Res. 2024 Jan 5:gkad1236. doi: 10.1093/nar/gkad1236."
COMMENT     Sequence Label: pAblo
FEATURES             Location/Qualifiers
     source          1..11383
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     oriT            454..562
                     /note="incP origin of transfer"
     rep_origin      656..1244
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     1145..1164
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(1298..2131)
                     /codon_start=1
                     /product="replication protein for the broad-host-range 
                     plasmid pRO1600 from Pseudomonas aeruginosa "
                     /label=pRO1600 Rep
                     /translation="MASPPMVYKSNALVEAAYRLSVQEQRIVLACISQVKRSEPVTDEV
                     MYSVTAEDIATMAGVPIESSYNQLKEAALRLKRREVRLTQEPNGKGKRPSVMITGWVQT
                     IIYREGEGRVELRFTKDMLPYLTELTKQFTKYALADVAKMDSTHAIRLYELLMQWDSIG
                     QREIEIDQLRKWFQLEGRYPSIKDFKLRVLDPAVTQINEHSPLQVEWAQRKTGRKVTHL
                     LFSFGPKKPAKAVGKAPAKRKAGKISDAEIAKQARPGETWEAARARLTQMPLDLA"
     rep_origin      2266..2617
                     /label=pRO1600 oriV
                     /note="broad-host-range origin of replication from 
                     Pseudomonas aeruginosa plasmid pRO1600; requires the 
                     pRO1600 Rep protein for replication (West et al., 1994)"
     terminator      complement(2690..2776)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     CDS             complement(2801..3766)
                     /codon_start=1
                     /gene="xylS"
                     /product="XylS regulator encoded by the Pseudomonas putida 
                     TOL plasmid pWWO"
                     /label=XylS
                     /note="stimulates transcription from the Pm promoter in the
                     presence of aromatic compounds such as m-toluate or 
                     benzoate"
                     /translation="MDFCLLNEKSQIFVHAEPYAVSDYVNQYVGTHSIRLPKGGRPAGR
                     LHHRIFGCLDLCRISYGGSVRVISPGLETCYHLQIILKGHCLWRGHGQEHYFAPGELLL
                     LNPDDQADLTYSEDCEKFIVKLPSVVLDRACSDNNWHKPREGIRFAARHNLQQLDGFIN
                     LLGLVCDEAEHTKSMPRVQEHYAGIIASKLLEMLGSNVSREIFSKGNPSFERVVQFIEE
                     NLKRNISLERLAELAMMSPRSLYNLFEKHAGTTPKNYIRNRKLESIRACLNDPSANVRS
                     ITEIALDYGFLHLGRFAENYRSAFGELPSDTLRQCKKEVA"
     promoter        4666..4746
                     /label=Pm promoter
                     /note="The bacterial Pm promoter is activated by XylS in 
                     the presence of benzoate or m-toluate (Marques et al., 
                     1999)."
     CDS             5403..7118
                     /codon_start=1
                     /product="N-terminal portion of Streptococcus pyogenes Cas9
                     (Zetsche et al., 2015)"
                     /label=Cas9(N)
                     /translation="DKKYSIGLAIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKKN
                     LIGALLFDSGETAERTRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEESF
                     LVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIKF
                     RGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRLE
                     NLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQI
                     GDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVRQ
                     QLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLLR
                     KQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARGN
                     SRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEYF
                     TVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIE"
     RBS             9560..9568
                     /label=Shine-Dalgarno sequence
                     /note="full consensus sequence for ribosome-binding sites 
                     upstream of start codons in E. coli; complementary to a 
                     region in the 3' end of the 16S rRNA (Chen et al., 1994)"
     terminator      10352..10446
                     /label=lambda t0 terminator
                     /note="transcription terminator from phage lambda"
     promoter        10469..10516
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     misc_RNA        10574..10649
                     /label=gRNA scaffold
                     /note="guide RNA scaffold for the Streptococcus pyogenes 
                     CRISPR/Cas9 system"
     CDS             join(10900..11383,1..308)
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
ORIGIN
        1 tatttgaggc gctaaatgaa accttaacgc tatggaactc gccgcccgac tgggctggcg
       61 atgagcgaaa tgtagtgctt acgttgtccc gcatttggta cagcgcagta accggcaaaa
      121 tcgcgccgaa ggatgtcgct gccgactggg caatggagcg cctgccggcc cagtatcagc
      181 ccgtcatact tgaagctaga caggcttatc ttggacaaga agaagatcgc ttggcctcgc
      241 gcgcagatca gttggaagaa tttgtccact acgtgaaagg cgagatcacc aaggtagtcg
      301 gcaaataaga caattgtcct tttccgctgc ataaccctgc ttcggggtca ttatagcgat
      361 tttttcggta tatccatcct ttttcgcacg atatacagga ttttgccaaa gggttcgtgt
      421 agactttcct tggtgtatcc aacggcgtca gccgggcagg ataggtgaag taggcccacc
      481 cgcgagcggg tgttccttct tcactgtccc ttattcgcac ctggcggtgc tcaacgggaa
      541 tcctgctctg cgaggctggc cgtaggccgg ccgataatct catgaccaaa atcccttaac
      601 gtgagttttc gttccactga gcgtcagacc ccgtagaaaa gatcaaagga tcttcttgag
      661 atcctttttt tctgcgcgta atctgctgct tgcaaacaaa aaaaccaccg ctaccagcgg
      721 tggtttgttt gccggatcaa gagctaccaa ctctttttcc gaaggtaact ggcttcagca
      781 gagcgcagat accaaatact gttcttctag tatagccgta gttaggccac cacttcaaga
      841 actctgtagc accgcctaca tacctcgctc tgctaatcct gttaccagtg gctgctgcca
      901 gtggcgataa gtcgtgtctt accgggttgg actcaagacg atagttaccg gataaggcgc
      961 agcggtcggg ctgaacgggg ggttcgtgca cacagcccag cttggagcga acgacctaca
     1021 ccgaactgag atacctacag cgtgagctat gagaaagcgc cacgcttccc gaagggagaa
     1081 aggcggacag gcatccggta agcggcaggg tcggaacagg agagcgcacg agggagcttc
     1141 cagggggaaa cgcctggtat ctttatagtc ctgtcgggtt tcgccacctc tgacttgagc
     1201 gtcgattttt gtgatgctcg tcaggggggc ggagcctatg gaaaaacgcc agcaacgcgg
     1261 ccgtgaaagg caggccggtc cgtggtggcc acggcctcta ggccagatcc agcggcatct
     1321 gggttagtcg agcgcgggcc gcttcccatg tctcaccagg gcgagcctgt ttcgcgatct
     1381 cagcatctga aatcttcccg gccttgcgct tcgctggggc cttacccacc gccttggcgg
     1441 gcttcttcgg tccaaaactg aacaacagat gtgtgacctt gcgcccggtc tttcgctgcg
     1501 cccactccac ctgtagcggg ctgtgctcgt tgatctgcgt cacggctgga tcaagcactc
     1561 gcaacttgaa gtccttgatc gagggatacc ggccttccag ttgaaaccac tttcgcagct
     1621 ggtcaatttc tatttcgcgc tggccgatgc tgtcccattg catgagcagc tcgtaaagcc
     1681 tgatcgcgtg ggtgctgtcc atcttggcca cgtcagccaa ggcgtatttg gtgaactgtt
     1741 tggtgagttc cgtcaggtac ggcagcatgt ctttggtgaa cctgagttct acacggccct
     1801 caccctcccg gtagatgatt gtttgcaccc agccggtaat catcacactc ggtcttttcc
     1861 ccttgccatt gggctcttgg gttaaccgga cttcccgccg tttcaggcgc agggccgctt
     1921 ctttgagctg gttgtaggaa gattcgatag ggacacccgc catcgtcgct atgtcctccg
     1981 ccgtcactga atacatcact tcatcggtga caggctcgct cctcttcacc tggctaatac
     2041 aggccagaac gatccgctgt tcctgaacac tgaggcgata cgcggcctcg accagggcat
     2101 tgcttttgta aaccattggg ggtgaggcca cgttcgacat atgtccgctc ccttctctga
     2161 cgccgtccac gccctaggca tggtcatgac tccattatta ttgtttctgt tgcataaagc
     2221 ctaaggggta ggccttacta gagatagcct tacgttagtt atccttgtgt ataaggggac
     2281 actgtatctg cgtcccacaa tacaacaaat ccgtcccttt acaacaacaa atccgtccct
     2341 tcttaacaac aaatccgtcc cttaatggca acaaatccgt ccctttttaa actctacagg
     2401 ccacggatta cgtggcctgt agacgtccta aaaggtttaa aagggaaaag gaagaaaagg
     2461 gtggaaacgc aaaaaacgca ccactacgtg gccccgttgg ggccgcattt gtgcccctga
     2521 aggggcgggg gaggcgtctg ggcaatcccc gttttaccag tcccctatcg ccgcctgaga
     2581 gggcgcagga agcgagtaat cagggtatcg aggcggattc acccttggcg tccaaccagc
     2641 ggcaccagcg gcgcctgaga ggggcgcgcc cagctgtcta gggcggcgga tttgtcctac
     2701 tcaggagagc gttcaccgac aaacaacaga taaaacgaaa ggcccagtct ttcgactgag
     2761 cttttcgttt tatttgatgc ctttaattaa acgttcgtaa tcaagccact tcctttttgc
     2821 attgacgcag ggtgtcggaa ggcaactcgc cgaacgcgct cctatagttt tcagcgaagc
     2881 gtcccaaatg taagaagccg tagtctaggg ctatctcagt tatactacgc acattggcac
     2941 tgggatcgtt caagcaggcg cggatgcttt cgagcttgcg gttgcggatg tagttcttcg
     3001 gcgtggtgcc ggcgtgcttc tcgaacaaat tgtagagcga gcgtggactc atcatcgcca
     3061 gctccgctaa ccgctcaagg ctgatattcc gtttgagatt ctcctcaatg aattgaacga
     3121 ctcgctcgaa agacgggtta cctttgctga aaatttcacg gctgacattg ctgcccagca
     3181 tttcgagcag cttggaagcg atgatccccg catagtgctc ttggacccga ggcatcgact
     3241 ttgtatgttc cgcttcgtca caaactaacc cgagtagatt gataaagcca tcgagttgct
     3301 ggagattgtg tcgcgcggcg aaacggatac cctccctcgg cttgtgccaa ttgttgtcac
     3361 tgcacgcccg atcaaggacc actgagggca atttaacgat aaatttctcg caatcttctg
     3421 aataggtcag gtcggcttgg tcatccggat tgagcagcaa tagttcgccc ggcgcaaaat
     3481 agtgctcctg gccatggcca cgccacaggc aatggccttt gagtattatt tgcagatgat
     3541 aacaggtttc taatccaggc gagattaccc tcacgctacc gccgtagctg attcgacaca
     3601 gatcgaggca tccgaagatt ctgtggtgca gcctgcctgc cgggcgcccg cccttgggca
     3661 ggcgaataga gtgcgtaccg acatactggt taacataatc ggagactgca tagggctcgg
     3721 cgtggacgaa gatctgactt ttctcgttca ataagcaaaa atccatagtt cacggttctc
     3781 ttattttaat gtgggctgct tggtgtgatg tagaaaggcg ccaagtcgat gaaaatgcat
     3841 ctcgacgtga tgcgtatacg ggttaccccc attgccacgt tgcgccatcc tttttgcaat
     3901 cagtgaccac ttttccaagc aaaaataacg ccaagcagaa cgaagacgtt ctttttaaga
     3961 agcgagaaca ccagaagttc gtgctgtcgg ggcatggggc gacgaattgg cggataaagg
     4021 ggatctgctg gatattacgg cctttttaaa gaccgtaaag aaaaataagc acaagtttta
     4081 tccggccttt attcacattc ttgcccgcct gatgaatgct catccgtaat tacgtatggc
     4141 aatgaaagac ggtgagctgg tgatatggga tagtgttcac ccttgttaca ccgttttcca
     4201 tgagcaaact gaaacgtttt catcgctctg gagtgaatac cacgacgatt tccggcagtt
     4261 tctacacata tattcgcaag atgtggcgtg ttacggtgaa aacctggcct atttccctaa
     4321 agggtttatt gagaatatgt ttttcgtctc agccaatccc tgggtgagtt tcaccagttt
     4381 tgatttaaac gtggccaata tggacaactt cttcgccccc gttttcacca tgcatgggaa
     4441 ttagcttgat ctgaccaacg accggtagcg gagctatcca acggcggtat accaggaaaa
     4501 cacacagcag gtacatcaga acagtaccat gactgaagaa caaatagttt tttcctgatc
     4561 cataaagcag aacggcctgc tccatgacaa atctggctcc ccaactaatg ccccatgcag
     4621 ccagcataac cagcataaag tgcagtgtcc ggtttgatag ggataagtcc agccttgcaa
     4681 gaagcggata caggagtgca aaaaatggct atctctagta aggcctaccc cttaggcttt
     4741 atgcaacaga aacaataata atggagtcat gaccatgcct aggccgcggt taggaggaaa
     4801 aacatatgag cgaagtcgag ttctcccatg agtattggat gcggcacgct ctgaccctcg
     4861 cgaaacgtgc tcgcgacgag cgggaagtcc cagtgggcgc tgtcctcgtg ctgaataacc
     4921 gcgtgattgg cgaaggctgg aatcgggcca tcggactcca tgatcctacg gcacacgcag
     4981 aaatcatggc cctgcgccag ggtggcctcg tcatgcaaaa ttatcggctg tacgacgcca
     5041 cgttgtattc caccttcgag ccctgcgtca tgtgtgccgg ggcaatgatt cactcccgta
     5101 ttgggcgtgt ggtgttcggg gtgcgcaatg caaagaccgg cgctgccggc tcgctcatgg
     5161 atgtcttgca ccatcccggt atgaaccacc gggtcgagat taccgaaggc attttggccg
     5221 acgagtgcgc cgcgctcttg tgccggtttt ttcgtatgcc acggcgtgtg ttcaacgctc
     5281 aaaagaaagc acaaagctcc accgattccg ggggttcctc gggcggctcg agcgggtccg
     5341 aaacccctgg tacgtcggaa tcggctacgc cagaatcgtc cggcggctcg tccggcggtt
     5401 ctgacaagaa gtacagcatc ggcctggcca tcggcaccaa ctctgtgggc tgggccgtga
     5461 tcaccgacga gtacaaggtg cccagcaaga aattcaaggt gctgggcaac accgaccggc
     5521 acagcatcaa gaagaacctg atcggagccc tgctgttcga cagcggcgaa acagccgaga
     5581 gaacccggct gaagagaacc gccagaagaa gatacaccag acggaagaac cggatctgct
     5641 atctgcaaga gatcttcagc aacgagatgg ccaaggtgga cgacagcttc ttccacagac
     5701 tggaagagtc cttcctggtg gaagaggata agaagcacga gcggcacccc atcttcggca
     5761 acatcgtgga cgaggtggcc taccacgaga agtaccccac catctaccac ctgagaaaga
     5821 aactggtgga cagcaccgac aaggccgacc tgcggctgat ctatctggcc ctggcccaca
     5881 tgatcaagtt ccggggccac ttcctgatcg agggcgacct gaaccccgac aacagcgacg
     5941 tggacaagct gttcatccag ctggtgcaga cctacaacca gctgttcgag gaaaacccca
     6001 tcaacgccag cggcgtggac gccaaggcca tcctgtctgc cagactgagc aagagcagac
     6061 ggctggaaaa tctgatcgcc cagctgcccg gcgagaagaa gaatggcctg ttcggaaacc
     6121 tgattgccct gagcctgggc ctgaccccca acttcaagag caacttcgac ctggccgagg
     6181 atgccaaact gcagctgagc aaggacacct acgacgacga cctggacaac ctgctggccc
     6241 agatcggcga ccagtacgcc gacctgtttc tggccgccaa gaacctgtcc gacgccatcc
     6301 tgctgagcga catcctgaga gtgaacaccg agatcaccaa ggcccccctg agcgcctcta
     6361 tgatcaagag atacgacgag caccaccagg acctgaccct gctgaaagct ctcgtgcggc
     6421 agcagctgcc tgagaagtac aaagagattt tcttcgacca gagcaagaac ggctacgccg
     6481 gctacattga cggcggagcc agccaggaag agttctacaa gttcatcaag cccatcctgg
     6541 aaaagatgga cggcaccgag gaactgctcg tgaagctgaa cagagaggac ctgctgcgga
     6601 agcagcggac cttcgacaac ggcagcatcc cccaccagat ccacctggga gagctgcacg
     6661 ccattctgcg gcggcaggaa gatttttacc cattcctgaa ggacaaccgg gaaaagatcg
     6721 agaagatcct gaccttccgc atcccctact acgtgggccc tctggccagg ggaaacagca
     6781 gattcgcctg gatgaccaga aagagcgagg aaaccatcac cccctggaac ttcgaggaag
     6841 tggtggacaa gggcgcttcc gcccagagct tcatcgagcg gatgaccaac ttcgataaga
     6901 acctgcccaa cgagaaggtg ctgcccaagc acagcctgct gtacgagtac ttcaccgtgt
     6961 ataacgagct gaccaaagtg aaatacgtga ccgagggaat gagaaagccc gctttcctga
     7021 gcggcgagca gaaaaaggcc atcgtggacc tgctgttcaa gaccaaccgg aaagtgaccg
     7081 tgaagcagct gaaagaggac tacttcaaga aaatcgagtg ttttgatagt gttgaaattt
     7141 caggagttga agatagattt aatgcttcat taggtaccta ccatgatttg ctaaaaatta
     7201 ttaaagataa agattttttg gataatgaag aaaatgaaga tatcttagag gatattgttt
     7261 taacattgac cttatttgaa gatagggaga tgattgagga aagacttaaa acatatgctc
     7321 acctctttga tgataaggtg ataaaacagc ttaaacgtcg ccgttatact ggttggggac
     7381 gtttgtctcg aaaattgatt aatggtatta gggataagca atctggcaaa acaatattag
     7441 attttttgaa atcagatggt tttgccaatc gcaattttat gcagctgatc catgatgata
     7501 gtttgacatt taaagaagac attcaaaaag cacaagtgtc tggacaaggc gatagtttac
     7561 atgaacatat tgcaaattta gctggtagcc ctgctattaa aaaaggtatt ttacagactg
     7621 taaaagttgt tgatgaattg gtcaaagtaa tggggcggca taagccagaa aatatcgtta
     7681 ttgaaatggc acgtgaaaat cagacaactc aaaagggcca gaaaaattcg cgagagcgta
     7741 tgaaacgaat cgaagaaggt atcaaagaat taggaagtca gattcttaaa gagcatcctg
     7801 ttgaaaatac tcaattgcaa aatgaaaagc tctatctcta ttatctccaa aatggaagag
     7861 acatgtatgt ggaccaagaa ttagatatta atcgtttaag tgattatgat gtcgatcaca
     7921 ttgttccaca aagtttcctt aaagacgatt caatagacaa taaggtctta acgcgttctg
     7981 ataaaaatcg tggtaaatcg gataacgttc caagtgaaga agtagtcaaa aagatgaaaa
     8041 actattggag acaacttcta aacgccaagt taatcactca acgtaagttt gataatttaa
     8101 cgaaagctga acgtggaggt ttgagtgaac ttgataaagc tggttttatc aaacgccaat
     8161 tggttgaaac tcgccaaatc actaagcatg tggcacaaat tttggatagt cgcatgaata
     8221 ctaaatacga tgaaaatgat aaacttattc gagaggttaa agtgattacc ttaaaatcta
     8281 aattagtttc tgacttccga aaagatttcc aattctataa agtacgtgag attaacaatt
     8341 accatcatgc ccatgatgcg tatctaaatg ccgtcgttgg aactgctttg attaagaaat
     8401 atccaaaact tgaatcggag tttgtctatg gtgattataa agtttatgat gttcgtaaaa
     8461 tgattgctaa gtctgagcaa gaaataggca aagcaaccgc aaaatatttc ttttactcta
     8521 atatcatgaa cttcttcaaa acagaaatta cacttgcaaa tggagagatt cgcaaacgcc
     8581 ctctaatcga aactaatggg gaaactggag aaattgtctg ggataaaggg cgagattttg
     8641 ccacagtgcg caaagtattg tccatgcccc aagtcaatat tgtcaagaaa acagaagtac
     8701 agacaggcgg attctccaag gagtcaatcc ggcccaagcg gaacagcgat aagctcatcg
     8761 cgcggaaaaa agattgggac cctaaaaagt atggggggtt cttgtggccg acggtcgctt
     8821 acagcgtctt ggtcgtggca aaggtcgaaa agggcaagtc gaaaaagctc aaatcggtca
     8881 aagagttgtt gggtattacg attatggagc ggtcctcgtt cgaaaagaac cccatcgact
     8941 ttctggaagc caaaggctat aaggaagtca agaaggatct catcatcaaa ttgccgaaat
     9001 attccctctt tgaattggaa aacggtcgta agcgtatgct ggcctcggcg aagcaactgc
     9061 agaaagggaa cgaattggcg ctcccttcca agtatgtgaa cttcctctac ctcgcgtcgc
     9121 actacgagaa gttgaaaggg tcgccagaag ataatgaaca aaagcagctc tttgtggagc
     9181 agcacaagca ttacctcgac gagattatcg agcaaattag cgagttcagc aaacgggtca
     9241 ttctcgccga cgccaatctc gacaaagtgc tgtccgcata caataagcat cgggacaaac
     9301 caattcggga gcaagccgaa aatatcatcc acctgtttac cttgacccgc ctgggtgccc
     9361 ctcgcgcgtt caagtacttt gataccacca tcgacccaaa acagtaccgg tccacgaagg
     9421 aggtcctcga tgctaccctc atccatcaga gcattaccgg cctctatgag acgcgcatcg
     9481 acctctcgca gctggggggt gattgatcta gagtctttat ttgacatgcg tgatgtttag
     9541 aattataatt tggggacctt aaggaggttt tctaatgatc atgggaattc ataaaggtga
     9601 agaactgttc accggtgttg ttccgatcct ggttgaactg gatggtgatg ttaacggcca
     9661 caaattctct gttcgtggtg aaggtgaagg tgatgcaacc aacggtaaac tgaccctgaa
     9721 attcatctgc actaccggta aactgccggt tccatggccg actctggtga ctaccctgac
     9781 ctatggtgtt cagtgttttt ctcgttaccc ggatcacatg aagcagcatg atttcttcaa
     9841 atctgcaatg ccggaaggtt atgtacagga gcgcaccatt tctttcaaag acgatggcac
     9901 ctacaaaacc cgtgcagagg ttaaatttga aggtgatact ctggtgaacc gtattgaact
     9961 gaaaggcatt gatttcaaag aggacggcaa catcctgggc cacaaactgg aatataactt
    10021 caactcccat aacgtttaca tcaccgcaga caaacagaag aacggtatca aagctaactt
    10081 caaaattcgc cataacgttg aagacggtag cgtacagctg gcggaccact accagcagaa
    10141 cactccgatc ggtgatggtc cggttctgct gccggataac cactacctgt ccacccagtc
    10201 taaactgtcc aaagacccga acgaaaagcg cgaccacatg gtgctgctgg agttcgttac
    10261 tgcagcaggt atcacgcacg gcatggatga actctacaaa taaaagcttg cggccgcgtc
    10321 gtgactggga aaaccctggc gactagtctt ggactcctgt tgatagatcc agtaatgacc
    10381 tcagaactcc atctggattt gttcagaacg ctcggttgcc gccgggcgtt ttttattggt
    10441 gagaatccag gggtcccttt aattaattgt tgacaattaa tcatcggcat agtatatcgg
    10501 catagtataa tacgacaagg tgaggaacta aacccctagg ccgcggccgc gcggagaccc
    10561 gagactggtc tcagttttag agctagaaat agcaagttaa aataaggcta gtccgttatc
    10621 aacttgaaaa agtggcaccg agtcggtgca attcgagctc ggtacccggg gatcccaata
    10681 attacgattt acgtatttaa atgaaccttg accgaacgca gcggtggtaa cggcgcagtg
    10741 gcggttttca tggcttgtta tgactgtttt tttggggtac agtctatgcc tcgggcatcc
    10801 aagcagcaag cgcgttacgc cgtgggtcga tgtttgatgt tatggagcag caacgatgtt
    10861 acgcagcagg gcagtcgccc taaaacaaag ttaaacatca tgagggaagc ggtgatcgcc
    10921 gaagtatcga ctcaactatc agaggtagtt ggcgtcatcg agcgccatct cgaaccgacg
    10981 ttgctggccg tacatttgta cggctccgca gtggatggcg gcctgaagcc acacagtgat
    11041 attgatttgc tggttacggt gaccgtaagg cttgatgaaa caacgcggcg agctttgatc
    11101 aacgaccttt tggaaacttc ggcttcccct ggagagagcg agattctccg cgctgtagaa
    11161 gtcaccattg ttgtgcacga cgacatcatt ccgtggcgtt atccagctaa gcgcgaactg
    11221 caatttggag aatggcagcg caatgacatt cttgcaggta tcttcgagcc agccacgatc
    11281 gacattgatc tggctatctt gctgacaaaa gcaagagaac atagcgttgc cttggtaggt
    11341 ccagcggcgg aggaactctt tgatccggtt cctgaacagg atc
//
