LOCUS       sequence-410867-       12464 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  R8.91 plasmid with mutated integrase (D64V).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 12464)
  AUTHORS   Chavez M, Rane DA, Chen X, Qi LS
  TITLE     Stable expression of large transgenes via the knock-in of an 
            integrase-deficient lentivirus.
  JOURNAL   Nat Biomed Eng. 2023 May 1. doi: 10.1038/s41551-023-01037-x.
  PUBMED    37127707
REFERENCE   2  (bases 1 to 12464)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat Biomed 
            Eng. 2023 May 1. doi: 10.1038/s41551-023-01037-x."
COMMENT     Sequence Label: pSLQ8001 - pCMV-R8.91 (D64V)
FEATURES             Location/Qualifiers
     source          1..12464
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      25..613
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     514..533
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     767..784
                     /label=L4440
                     /note="L4440 vector, forward primer"
     primer_bind     complement(854..874)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        859..1188
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      1039..1174
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     intron          1393..1458
                     /label=small t intron
                     /note="SV40 (simian virus 40) small t antigen intron"
     CDS             1588..1608
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     enhancer        2062..2445
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        2446..2649
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     2600..2620
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     2646..2670
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     CDS             2924..4426
                     /codon_start=1
                     /gene="gag"
                     /product="gag protein from human immunodeficiency virus 1"
                     /label=HIV-1 gag
                     /translation="MGARASVLSGGELDRWEKIRLRPGGKKKYKLKHIVWASRELERFA
                     VNPGLLETSEGCRQILGQLQPSLQTGSEELRSLYNTVATLYCVHQRIEIKDTKEALDKI
                     EEEQNKSKKKAQQAAADTGHSNQVSQNYPIVQNIQGQMVHQAISPRTLNAWVKVVEEKA
                     FSPEVIPMFSALSEGATPQDLNTMLNTVGGHQAAMQMLKETINEEAAEWDRLHPVQAGP
                     VAPGQMREPRGSDIAGTTSTLQEQIGWMTHNPPIPVGEIYKRWIILGLNKIVRMYSPTS
                     ILDIRQGPKEPFRDYVDRFYKTLRAEQASQEVKNWMTETLLVQNANPDCKTILKALGPG
                     ATLEEMMTACQGVGGPGHKARVLAEAMSQVTNPATIMIQKGNFRNQRKTVKCFNCGKEG
                     HIAKNCRAPRKKGCWKCGKEGHQMKDCTERQANFLGKIWPSHKGRPGNFLQSRPEPTAP
                     PEESFRFGEETTTPSQKQEPIDKELYPLASLRSLFGSDPSSQ"
     CDS             4219..7230
                     /codon_start=1
                     /gene="pol"
                     /product="pol protein from human immunodeficiency virus 1"
                     /label=HIV-1 pol
                     /translation="FFREDLAFPQGKAREFSSEQTRANSPTRRELQVWGRDNNSLSEAG
                     ADRQGTVSFSFPQITLWQRPLVTIKIGGQLKEALLDTGADDTVLEEMNLPGRWKPKMIG
                     GIGGFIKVRQYDQILIEICGHKAIGTVLVGPTPVNIIGRNLLTQIGCTLNFPISPIETV
                     PVKLKPGMDGPKVKQWPLTEEKIKALVEICTEMEKEGKISKIGPENPYNTPVFAIKKKD
                     STKWRKLVDFRELNKRTQDFWEVQLGIPHPAGLKQKKSVTVLDVGDAYFSVPLDKDFRK
                     YTAFTIPSINNETPGIRYQYNVLPQGWKGSPAIFQCSMTKILEPFRKQNPDIVIYQYMD
                     DLYVGSDLEIGQHRTKIEELRQHLLRWGFTTPDKKHQKEPPFLWMGYELHPDKWTVQPI
                     VLPEKDSWTVNDIQKLVGKLNWASQIYAGIKVRQLCKLLRGTKALTEVVPLTEEAELEL
                     AENREILKEPVHGVYYDPSKDLIAEIQKQGQGQWTYQIYQEPFKNLKTGKYARMKGAHT
                     NDVKQLTEAVQKIATESIVIWGKTPKFKLPIQKETWEAWWTEYWQATWIPEWEFVNTPP
                     LVKLWYQLEKEPIIGAETFYVDGAANRETKLGKAGYVTDRGRQKVVPLTDTTNQKTELQ
                     AIHLALQDSGLEVNIVTDSQYALGIIQAQPDKSESELVSQIIEQLIKKEKVYLAWVPAH
                     KGIGGNEQVDKLVSAGIRKVLFLDGIDKAQEEHEKYHSNWRAMASDFNLPPVVAKEIVA
                     SCDKCQLKGEAMHGQVDCSPGIWQLVCTHLEGKVILVAVHVASGYIEAEVIPAETGQET
                     AYFLLKLAGRWPVKTVHTDNGSNFTSTTVKAACWWAGIKQEFGIPYNPQSQGVIESMNK
                     ELKKIIGQVRDQAEHLKTAVQMAVFIHNFKRKGGIGGYSAGERIVDIIATDIQTKELQK
                     QITKIQNFRVYYRDSRDPVWKGPAKLLWKGEGAVVIQDNSDIKVVPRRKAKIIRDYGKQ
                     MAGDDCVASRQDED"
     misc_feature    6915..7032
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     misc_feature    7804..8037
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             8222..8266
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     CDS             9898..9924
                     /codon_start=1
                     /product="nuclear export signal from the HIV Rev protein 
                     (Fischer et al., 1995)"
                     /label=NES
                     /translation="LPPLERLTL"
     promoter        complement(10547..10565)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(10548..10565)
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     complement(10855..10874)
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
     primer_bind     complement(11088..11107)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     11207..11229
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     promoter        11353..11457
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(11458..11526,11527..12318)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(11676..11695)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
ORIGIN
        1 cgtagaaaag atcaaaggat cttcttgaga tccttttttt ctgcgcgtaa tctgctgctt
       61 gcaaacaaaa aaaccaccgc taccagcggt ggtttgtttg ccggatcaag agctaccaac
      121 tctttttccg aaggtaactg gcttcagcag agcgcagata ccaaatactg ttcttctagt
      181 gtagccgtag ttaggccacc acttcaagaa ctctgtagca ccgcctacat acctcgctct
      241 gctaatcctg ttaccagtgg ctgctgccag tggcgataag tcgtgtctta ccgggttgga
      301 ctcaagacga tagttaccgg ataaggcgca gcggtcgggc tgaacggggg gttcgtgcac
      361 acagcccagc ttggagcgaa cgacctacac cgaactgaga tacctacagc gtgagctatg
      421 agaaagcgcc acgcttcccg aagggagaaa ggcggacagg tatccggtaa gcggcagggt
      481 cggaacagga gagcgcacga gggagcttcc agggggaaac gcctggtatc tttatagtcc
      541 tgtcgggttt cgccacctct gacttgagcg tcgatttttg tgatgctcgt caggggggcg
      601 gagcctatgg aaaaacgcca gcaacgcggc ctttttacgg ttcctggcct tttgctggcc
      661 ttttgctcac atgttctttc ctgcgttatc ccctgattct gtggataacc gtattaccgc
      721 ctttgagtga gctgataccg ctcgccgcag ccgaacgacc gagcgcagcg agtcagtgag
      781 cgaggaagcg gaagagcgcc caatacgcaa accgcctctc cccgcgcgtt ggccgattca
      841 ttaatgcagc tgtggaatgt gtgtcagtta gggtgtggaa agtccccagg ctccccagca
      901 ggcagaagta tgcaaagcat gcatctcaat tagtcagcaa ccaggtgtgg aaagtcccca
      961 ggctccccag caggcagaag tatgcaaagc atgcatctca attagtcagc aaccatagtc
     1021 ccgcccctaa ctccgcccat cccgccccta actccgccca gttccgccca ttctccgccc
     1081 catggctgac taattttttt tatttatgca gaggccgagg ccgcctcggc ctctgagcta
     1141 ttccagaagt agtgaggagg cttttttgga ggcctaggct tttgcaaaaa gcttggacac
     1201 aagacaggct tgcgagatat gtttgagaat accactttat cccgcgtcag ggagaggcag
     1261 tgcgtaaaaa gacgcggact catgtgaaat actggttttt agtgcgccag atcccggatc
     1321 tttgtgaagg aaccttactt ctgtggtgtg acataattgg acaaactacc tacagagatt
     1381 taaagctcta aggtaaatat aaaattttta agtgtataat gtgttaaact actgattcta
     1441 attgtttgtg tattttagat tccaacctat ggaactgatg aatgggagca gtggtggaat
     1501 gcctttaatg aggaaaacct gttttgctca gaagaaatgc catctagtga tgatgaggct
     1561 actgctgact ctcaacattc tactcctcca aaaaagaaga gaaaggtaga agaccccaag
     1621 gactttcctt cagaattgct aagttttttg agtcatgctg tgtttagtaa tagaactctt
     1681 gcttgctttg ctatttacac cacaaaggaa aaagctgcac tgctatacaa gaaaattatg
     1741 gaaaaatatt ctgtaacctt tataagtagg cataacagtt ataatcataa catactgttt
     1801 tttcttactc cacacaggca tagagtgtct gctattaata actatgctca aaaattgtgt
     1861 acctttagct ttttaatttg taaaggggtt aataaggaat atttgatgta tagtgccttg
     1921 actagagatc ataatcagcc ataccacatt tgtagaggtt ttacttgctt taaaaaacct
     1981 cccacacctc cccctgaacc tgaaacataa aatgaatgca attgttgttg ttgggctgca
     2041 ggaattaatt cgagctcgcc cgacattgat tattgactag ctagttatta atagtaatca
     2101 attacggggt cattagttca tagcccatat atggagttcc gcgttacata acttacggta
     2161 aatggcccgc ctggctgacc gcccaacgac ccccgcccat tgacgtcaat aatgacgtat
     2221 gttcccatag taacgccaat agggactttc cattgacgtc aatgggtgga gtatttacgg
     2281 taaactgccc acttggcagt acatcaagtg tatcatatgc caagtacgcc ccctattgac
     2341 gtcaatgacg gtaaatggcc cgcctggcat tatgcccagt acatgacctt atgggacttt
     2401 cctacttggc agtacatcta cgtattagtc atcgctatta ccatggtgat gcggttttgg
     2461 cagtacatca atgggcgtgg atagcggttt gactcacggg gatttccaag tctccacccc
     2521 attgacgtca atgggagttt gttttggcac caaaatcaac gggactttcc aaaatgtcgt
     2581 aacaactccg ccccattgac gcaaatgggc ggtaggcgtg tacggtggga ggtctatata
     2641 agcagagctc gtttagtgaa ccgtcagatc gcctggagac gccatccacg ctgttttgac
     2701 ctccatagaa gacaccggga ccgatccagc ctccgcggcc gggaacggtg cattggaacg
     2761 cggattcccc gtgccaagag tgacgtaagt accgcctata gagtctatag gcccaccccc
     2821 ttggcttctt atgcgacgga tcgatcccgt aataagcttc gaggtccgcg gccggccgcg
     2881 ttgacgcgca cggcaagagg cgaggggcgg ccgctggtga gagatgggtg cgagagcgtc
     2941 agtattaagc gggggagaat tagatcgatg ggaaaaaatt cggttaaggc cagggggaaa
     3001 gaaaaaatat aaattaaaac atatagtatg ggcaagcagg gagctagaac gattcgcagt
     3061 taatcctggc ctgttagaaa catcagaagg ctgtagacaa atactgggac agctacaacc
     3121 atcccttcag acaggatcag aagaacttag atcattatat aatacagtag caaccctcta
     3181 ttgtgtgcat caaaggatag agataaaaga caccaaggaa gctttagaca agatagagga
     3241 agagcaaaac aaaagtaaga aaaaagcaca gcaagcagca gctgacacag gacacagcaa
     3301 tcaggtcagc caaaattacc ctatagtgca gaacatccag gggcaaatgg tacatcaggc
     3361 catatcacct agaactttaa atgcatgggt aaaagtagta gaagagaagg ctttcagccc
     3421 agaagtgata cccatgtttt cagcattatc agaaggagcc accccacaag atttaaacac
     3481 catgctaaac acagtggggg gacatcaagc agccatgcaa atgttaaaag agaccatcaa
     3541 tgaggaagct gcagaatggg atagattgca tccagtgcag gcagggcctg ttgcaccagg
     3601 ccagatgaga gaaccaaggg gaagtgacat agcaggaact actagtaccc ttcaggaaca
     3661 aataggatgg atgacacata atccacctat cccagtagga gaaatctata aaagatggat
     3721 aatcctggga ttaaataaaa tagtaagaat gtatagccct accagcattc tggacataag
     3781 acaaggacca aaggaaccct ttagagacta tgtagaccga ttctataaaa ctctaagagc
     3841 cgagcaagct tcacaagagg taaaaaattg gatgacagaa accttgttgg tccaaaatgc
     3901 gaacccagat tgtaagacta ttttaaaagc attgggacca ggagcgacac tagaagaaat
     3961 gatgacagca tgtcagggag tggggggacc cggccataaa gcaagagttt tggctgaagc
     4021 aatgagccaa gtaacaaatc cagctaccat aatgatacag aaaggcaatt ttaggaacca
     4081 aagaaagact gttaagtgtt tcaattgtgg caaagaaggg cacatagcca aaaattgcag
     4141 ggcccctagg aaaaagggct gttggaaatg tggaaaggaa ggacaccaaa tgaaagattg
     4201 tactgagaga caggctaatt ttttagggaa gatctggcct tcccacaagg gaaggccagg
     4261 gaattttctt cagagcagac cagagccaac agccccacca gaagagagct tcaggtttgg
     4321 ggaagagaca acaactccct ctcagaagca ggagccgata gacaaggaac tgtatccttt
     4381 agcttccctc agatcactct ttggcagcga cccctcgtca caataaagat aggggggcaa
     4441 ttaaaggaag ctctattaga tacaggagca gatgatacag tattagaaga aatgaatttg
     4501 ccaggaagat ggaaaccaaa aatgataggg ggaattggag gttttatcaa agtaagacag
     4561 tatgatcaga tactcataga aatctgcgga cataaagcta taggtacagt attagtagga
     4621 cctacacctg tcaacataat tggaagaaat ctgttgactc agattggctg cactttaaat
     4681 tttcccatta gtcctattga gactgtacca gtaaaattaa agccaggaat ggatggccca
     4741 aaagttaaac aatggccatt gacagaagaa aaaataaaag cattagtaga aatttgtaca
     4801 gaaatggaaa aggaaggaaa aatttcaaaa attgggcctg aaaatccata caatactcca
     4861 gtatttgcca taaagaaaaa agacagtact aaatggagaa aattagtaga tttcagagaa
     4921 cttaataaga gaactcaaga tttctgggaa gttcaattag gaataccaca tcctgcaggg
     4981 ttaaaacaga aaaaatcagt aacagtactg gatgtgggcg atgcatattt ttcagttccc
     5041 ttagataaag acttcaggaa gtatactgca tttaccatac ctagtataaa caatgagaca
     5101 ccagggatta gatatcagta caatgtgctt ccacagggat ggaaaggatc accagcaata
     5161 ttccagtgta gcatgacaaa aatcttagag ccttttagaa aacaaaatcc agacatagtc
     5221 atctatcaat acatggatga tttgtatgta ggatctgact tagaaatagg gcagcataga
     5281 acaaaaatag aggaactgag acaacatctg ttgaggtggg gatttaccac accagacaaa
     5341 aaacatcaga aagaacctcc attcctttgg atgggttatg aactccatcc tgataaatgg
     5401 acagtacagc ctatagtgct gccagaaaag gacagctgga ctgtcaatga catacagaaa
     5461 ttagtgggaa aattgaattg ggcaagtcag atttatgcag ggattaaagt aaggcaatta
     5521 tgtaaacttc ttaggggaac caaagcacta acagaagtag taccactaac agaagaagca
     5581 gagctagaac tggcagaaaa cagggagatt ctaaaagaac cggtacatgg agtgtattat
     5641 gacccatcaa aagacttaat agcagaaata cagaagcagg ggcaaggcca atggacatat
     5701 caaatttatc aagagccatt taaaaatctg aaaacaggaa agtatgcaag aatgaagggt
     5761 gcccacacta atgatgtgaa acaattaaca gaggcagtac aaaaaatagc cacagaaagc
     5821 atagtaatat ggggaaagac tcctaaattt aaattaccca tacaaaagga aacatgggaa
     5881 gcatggtgga cagagtattg gcaagccacc tggattcctg agtgggagtt tgtcaatacc
     5941 cctcccttag tgaagttatg gtaccagtta gagaaagaac ccataatagg agcagaaact
     6001 ttctatgtag atggggcagc caatagggaa actaaattag gaaaagcagg atatgtaact
     6061 gacagaggaa gacaaaaagt tgtcccccta acggacacaa caaatcagaa gactgagtta
     6121 caagcaattc atctagcttt gcaggattcg ggattagaag taaacatagt gacagactca
     6181 caatatgcat tgggaatcat tcaagcacaa ccagataaga gtgaatcaga gttagtcagt
     6241 caaataatag agcagttaat aaaaaaggaa aaagtctacc tggcatgggt accagcacac
     6301 aaaggaattg gaggaaatga acaagtagat aaattggtca gtgctggaat caggaaagta
     6361 ctatttttag atggaataga taaggcccaa gaagaacatg agaaatatca cagtaattgg
     6421 agagcaatgg ctagtgattt taacctacca cctgtagtag caaaagaaat agtagccagc
     6481 tgtgataaat gtcagctaaa aggggaagcc atgcatggac aagtagactg tagcccagga
     6541 atatggcagc tagtatgtac acatttagaa ggaaaagtta tcttggtagc agttcatgta
     6601 gccagtggat atatagaagc agaagtaatt ccagcagaga cagggcaaga aacagcatac
     6661 ttcctcttaa aattagcagg aagatggcca gtaaaaacag tacatacaga caatggcagc
     6721 aatttcacca gtactacagt taaggccgcc tgttggtggg cggggatcaa gcaggaattt
     6781 ggcattccct acaatcccca aagtcaagga gtaatagaat ctatgaataa agaattaaag
     6841 aaaattatag gacaggtaag agatcaggct gaacatctta agacagcagt acaaatggca
     6901 gtattcatcc acaattttaa aagaaaaggg gggattgggg ggtacagtgc aggggaaaga
     6961 atagtagaca taatagcaac agacatacaa actaaagaat tacaaaaaca aattacaaaa
     7021 attcaaaatt ttcgggttta ttacagggac agcagagatc cagtttggaa aggaccagca
     7081 aagctcctct ggaaaggtga aggggcagta gtaatacaag ataatagtga cataaaagta
     7141 gtgccaagaa gaaaagcaaa gatcatcagg gattatggaa aacagatggc aggtgatgat
     7201 tgtgtggcaa gtagacagga tgaggattaa cacatggaat tctgcaacaa ctgctgttta
     7261 tccatttcag aattgggtgt cgacatagca gaataggcgt tactcgacag aggagagcaa
     7321 gaaatggagc cagtagatcc tagactagag ccctggaagc atccaggaag tcagcctaaa
     7381 actgcttgta ccaattgcta ttgtaaaaag tgttgctttc attgccaagt ttgtttcatg
     7441 acaaaagcct taggcatctc ctatggcagg aagaagcgga gacagcgacg aagagctcat
     7501 cagaacagtc agactcatca agcttctcta tcaaagcagt aagtagtaca tgtaatgcaa
     7561 cctataatag tagcaatagt agcattagta gtagcaataa taatagcaat agttgtgtgg
     7621 tccatagtaa tcatagaata taggaaaatg gccgctgatc ttcagacctg gaggaggaga
     7681 tatgagggac aattggagaa gtgaattata taaatataaa gtagtaaaaa ttgaaccatt
     7741 aggagtagca cccaccaagg caaagagaag agtggtgcag agagaaaaaa gagcagtggg
     7801 aataggagct ttgttccttg ggttcttggg agcagcagga agcactatgg gcgcagcgtc
     7861 aatgacgctg acggtacagg ccagacaatt attgtctggt atagtgcagc agcagaacaa
     7921 tttgctgagg gctattgagg cgcaacagca tctgttgcaa ctcacagtct ggggcatcaa
     7981 gcagctccag gcaagaatcc tggctgtgga aagataccta aaggatcaac agctcctggg
     8041 gatttggggt tgctctggaa aactcatttg caccactgct gtgccttgga atgctagttg
     8101 gagtaataaa tctctggaac agatttggaa tcacacgacc tggatggagt gggacagaga
     8161 aattaacaat tacacaagct taatacactc cttaattgaa gaatcgcaaa accagcaaga
     8221 aaagaatgaa caagaattat tggaattaga taaatgggca agtttgtgga attggtttaa
     8281 cataacaaat tggctgtggt atataaaatt attcataatg atagtaggag gcttggtact
     8341 gatgaatccc ctaatgattt tggtaaaaat cattaagtta aggtggatac acatcttgtc
     8401 atatgatcaa atggtttcgc gaaaaatcaa taatcagaca acaagatgtg cgaactcgat
     8461 attttacacg actctcttta ccaattctgc cccgaattac acttaaaacg actcaacagc
     8521 ttaacgttgg cttgccacgc attacttgac tgtaaaactc tcactcttac cgaacttggc
     8581 cgtaacctgc caaccaaagc gagaacaaaa cataacatca aacgaatcga ccgattgtta
     8641 ggtaatcgtc acctccacaa agagcgactc gctgtatacc gttggcatgc tagctttatc
     8701 tgttcgggca atacgatgcc cattgtactt gttgactggt ctgatattcg tgagcaaaaa
     8761 cgacttatgg tattgcgagc ttcagtcgca ctacacggtc gttctgttac tctttatgag
     8821 aaagcgttcc cgctttcaga gcaatgttca aagaaagctc atgaccaatt tctagccgac
     8881 cttgcgagca ttctaccgag taacaccaca ccgctcattg tcagtgatgc tggctttaaa
     8941 gtgccatggt ataaatccgt tgagaagctg ggttggtact ggttaagtcg agtaagagga
     9001 aaagtacaat atgcagacct aggagcggaa aactggaaac ctatcagcaa cttacatgat
     9061 atgtcatcta gtcactcaaa gactttaggc tataagaggc tgactaaaag caatccaatc
     9121 tcatgccaaa ttctattgta taaatctcgc tctaaaggcc gaaaaaatca gcgctcgaca
     9181 cggactcatt gtcaccaccc gtcacctaaa atctactcag cgtcggcaaa ggagccatgg
     9241 gttctagcaa ctaacttacc tgttgaaatt cgaacaccca aacaacttgt taatatctat
     9301 tcgaagcgaa tgcagattga agaaaccttc cgagacttga aaagtcctgc ctacggacta
     9361 ggcctacgcc atagccgaac gagcagctca gagcgttttg atatcatgct gctaatcgcc
     9421 ctgatgcttc aactaacatg ttggcttgcg ggcgttcatg ctcagaaaca aggttgggac
     9481 aagcacttcc aggctaacac agtcagaaat cgaaacgtac tctcaacagt tcgcttaggc
     9541 atggaagttt tgcggcattc tggctacaca ataacaaggg aagacttact cgtggctgca
     9601 accctactag ctcaaaattt attcacacat ggttacgctt tggggaaatt atgaggggat
     9661 ctctcagggc ttggtaggtt taagaatagt ttttgctgta ctttctatag tgaatagagt
     9721 taggcaggga tattcaccat tatcgtttca gacccacctc ccaaccccga ggggacccga
     9781 caggcccgaa ggaatagaag aagaaggtgg agagagagac agagacagat ccattcgatt
     9841 agtgaacgga tccttggcac ttatctggga cgatctgcgg agcctgtgcc tcttcagcta
     9901 ccaccgcttg agagacttac tcttgattgt aacgaggatt gtggaacttc tgggacgcag
     9961 ggggtgggaa gccctcaaat attggtggaa tctcctacaa tattggagtc aggagctaaa
    10021 gaatagtgct gttagcttgc tcaatgccac agccatagca gtagctgagg ggacagatag
    10081 ggttatagaa gtagtacaag gagcttgtag agctattcgc cacataccta gaagaataag
    10141 acagggcttg gaaaggattt tgctataagc tcgaggccgc cccggtgacc ttcagacctt
    10201 ggcactggag gtggcccggc agaagcgcgg catcgtggat cagtgctgca ccagcatctg
    10261 ctctctctac caactggaga actactgcaa ctaggcccac cactaccctg tccacccctc
    10321 tgcaatgaat aaaacctttg aaagagcact acaagttgtg tgtacatgcg tgcatgtgca
    10381 tatgtggtgc ggggggaaca tgagtggggc tggctggagt ggcgatgata agctgtcaaa
    10441 catgagaatt aattcttgaa gacgaaaggg cctcgtgata cgcctatttt tataggttaa
    10501 tgtcatgata ataatggttt cttagtctag aattaattcc gtgtattcta tagtgtcacc
    10561 taaatcgtat gtgtatgata cataaggtta tgtattaatt gtagccgcgt tctaacgaca
    10621 atatgtacaa gcctaattgt gtagcatctg gcttactgaa gcagacccta tcatctctct
    10681 cgtaaactgc cgtcagagtc ggtttggttg gacgaacctt ctgagtttct ggtaacgccg
    10741 tcccgcaccc ggaaatggtc agcgaaccaa tcagcagggt catcgctagc cagatcctct
    10801 acgccggacg catcgtggcc ggcatcaccg gcgccacagg tgcggttgct ggcgcctata
    10861 tcgccgacat caccgatggg gaagatcggg ctcgccactt cgggctcatg agcgcttgtt
    10921 tcggcgtggg tatggtggca ggccccgtgg ccgggggact gttgggcgcc atctccttgc
    10981 atgcaccatt ccttgcggcg gcggtgctca acggcctcaa cctactactg ggctgcttcc
    11041 taatgcagga gtcgcataag ggagagcgtc gaatggtgca ctctcagtac aatctgctct
    11101 gatgccgcat agttaagcca gccccgacac ccgccaacac ccgctgacgc gccctgacgg
    11161 gcttgtctgc tcccggcatc cgcttacaga caagctgtga ccgtctccgg gagctgcatg
    11221 tgtcagaggt tttcaccgtc atcaccgaaa cgcgcgagac gaaagggcct cgtgatacgc
    11281 ctatttttat aggttaatgt catgataata atggtttctt agacgtcagg tggcactttt
    11341 cggggaaatg tgcgcggaac ccctatttgt ttatttttct aaatacattc aaatatgtat
    11401 ccgctcatga gacaataacc ctgataaatg cttcaataat attgaaaaag gaagagtatg
    11461 agtattcaac atttccgtgt cgcccttatt cccttttttg cggcattttg ccttcctgtt
    11521 tttgctcacc cagaaacgct ggtgaaagta aaagatgctg aagatcagtt gggtgcacga
    11581 gtgggttaca tcgaactgga tctcaacagc ggtaagatcc ttgagagttt tcgccccgaa
    11641 gaacgttttc caatgatgag cacttttaaa gttctgctat gtggcgcggt attatcccgt
    11701 attgacgccg ggcaagagca actcggtcgc cgcatacact attctcagaa tgacttggtt
    11761 gagtactcac cagtcacaga aaagcatctt acggatggca tgacagtaag agaattatgc
    11821 agtgctgcca taaccatgag tgataacact gcggccaact tacttctgac aacgatcgga
    11881 ggaccgaagg agctaaccgc ttttttgcac aacatggggg atcatgtaac tcgccttgat
    11941 cgttgggaac cggagctgaa tgaagccata ccaaacgacg agcgtgacac cacgatgcct
    12001 gtagcaatgg caacaacgtt gcgcaaacta ttaactggcg aactacttac tctagcttcc
    12061 cggcaacaat taatagactg gatggaggcg gataaagttg caggaccact tctgcgctcg
    12121 gcccttccgg ctggctggtt tattgctgat aaatctggag ccggtgagcg tgggtctcgc
    12181 ggtatcattg cagcactggg gccagatggt aagccctccc gtatcgtagt tatctacacg
    12241 acggggagtc aggcaactat ggatgaacga aatagacaga tcgctgagat aggtgcctca
    12301 ctgattaagc attggtaact gtcagaccaa gtttactcat atatacttta gattgattta
    12361 aaacttcatt tttaatttaa aaggatctag gtgaagatcc tttttgataa tctcatgacc
    12421 aaaatccctt aacgtgagtt ttcgttccac tgagcgtcag accc
//
