LOCUS       sequence-410901-         867 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses a nuclear-targeted actin filament reporter, consisting of 
            the first 230 amino acids of human utrophin, on a CMV promoter.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 867)
  AUTHORS   Belin BJ, Cimini BA, Blackburn EH, Mullins RD
  TITLE     Visualization of actin filaments and monomers in somatic cell 
            nuclei.
  JOURNAL   Mol Biol Cell. 2013 Apr;24(7):982-94. doi: 10.1091/mbc.E12-09-0685. 
            Epub 2013 Feb 27.
  PUBMED    23447706
REFERENCE   2  (bases 1 to 867)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1091/mbc.E12-09-0685"; journalName: "Mol Biol Cell"; date: 
            "2013-04"; volume: "24"; issue: "7"; pages: "982-94"
COMMENT     Sequence Label: pEGFP-C1 Utr230-EGFP-3XNLS
FEATURES             Location/Qualifiers
     source          1..867
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      7..16
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     promoter        complement(756..855)
                     /label=CMVd1 promoter
                     /note="truncated human cytomegalovirus (CMV) immediate 
                     early promoter that yields slightly reduced expression 
                     relative to the full-length promoter"
     primer_bind     complement(785..805)
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
ORIGIN
        1 tgctcaccat ggtggcgacc ggtacccccc cccccccagg atctaacagc ttttcaattc
       61 ccaaataagt ttgagccttg ctgaaggcat gttcaagtct ctcaattggt gacattttga
      121 caactttatc ccagctgaag agatcaggtt tatgtcggtg gaggacagca ttaaaggcga
      181 gtccatctgt ccagctggtg gtgaagttga ggacgttgac ttggctgtag ggcctggtgg
      241 tctgacgcac ccagctgagc aggatcttct cactgttcgt ctgctgcagg tccgacatga
      301 catccttcat gacatctttc acctgccagt gcaaaatgat gctccaaagt aaccccaaag
      361 tcagtttgtg atttccatcc acaatgtcag ttccccctat attcactaat tccacattgt
      421 tctgatgtaa aacctgcagc actctgttga cgttatttaa ggcatgtacc cttgtggaac
      481 cacgttcctt tggcagtgat gttcctgtga ggccttctag aagatccaat agcttccttc
      541 catctttgag gtctgtgaac atatcattga tgggtggttt cccactcttt gaaaatcgag
      601 catttatcca tttggtaaag gttttcttct gtacgtcatt gtgttcatca gatctggact
      661 taatgatatc actgaattcg ttctgcccat tgtcaggact ggcttcatgt tctccatact
      721 tggccatgct agcggatctg acggttcact aaaccagctc tgcttatata gacctcccac
      781 cgtacacgcc taccgcccat ttgcgtcaat ggggcggagt tgttacgaca ttttggaaag
      841 tcccgttgat tttggtgcca aaacaaa
//
