LOCUS       sequence-410913-        2209 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Level 0 plasmid containing a kozak consensus sequence and a pyrenoid
            compartment signal peptide sequence with BC overhangs used to build 
            level 1 constructs..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2209)
  AUTHORS   Pollak B, Matute T, Nunez I, Cerda A, Lopez C, Vargas V, Kan A, 
            Bielinski V, von Dassow P, Dupont CL, Federici F
  TITLE     Universal loop assembly: open, efficient and cross-kingdom DNA 
            fabrication.
  JOURNAL   Synth Biol (Oxf). 2020;5(1):ysaa001. doi: 10.1093/synbio/ysaa001. 
            Epub 2020 Feb 5.
  PUBMED    32161816
REFERENCE   2  (bases 1 to 2209)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Synth Biol 
            (Oxf)."; date: "2020"; volume: "5(1):ysaa001. doi"; pages: " 
            10.1093/synbio/ysaa001. Epub 2020 Feb 5"
COMMENT     Sequence Label: pL0_BC-pyr_comp_sig_pep
FEATURES             Location/Qualifiers
     source          1..2209
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      complement(1364..1952)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(1444..1463)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(2043..2209,1..625))
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
ORIGIN
        1 cagcgacatc cttcggcgcg attttgccgg ttactgcgct gtaccaaatg cgggacaacg
       61 taagcactac atttcgctca tcgccagccc agtcgggcgg cgagttccat agcgttaagg
      121 tttcatttag cgcctcaaat agatcctgtt caggaaccgg atcaaagagt tcctccgccg
      181 ctggacctac caaggcaacg ctatgttctc ttgcttttgt cagcaagata gccagatcaa
      241 tgtcgatcgt ggctggctcg aagataccag caagaatgtc attgcgctgc cattctccaa
      301 attgcagttc gcgcttagct ggataacgcc acggaatgat gtcgtcgtgc acaacaatgg
      361 tgacttctac agcgcggaga atctcactct ctccagggga agccgaagtt tccaaaaggt
      421 cgttgatcaa agctcgccgc gttgtttcat caagccttac ggtcaccgta accagcaaat
      481 caatatcact gtgtggcttc aggccgccat ccactgcgga gccgtacaaa tgtacggcca
      541 gcaacgtcgg ttcgagatgg cgctcgatga cgccaactac ctctgatagt tgagtcgata
      601 cttcggcgat caccgcttcc ctcatgcgaa acgatcctca tcctgtctct tgatcagata
      661 ttgatcccct gcgccatcag atccttggcg gcaagaaagc catccagttt actttgcagg
      721 gcttcccaac cttaccagag ggcgccccag ctggcaattc cggttcgctt gctaagcttg
      781 catgcctgca agtcgactct agaggagcgg tgatcacagg cagcaacgct ctgtcatcgt
      841 tacaatcaac atgctaccct ccgcgagatc atccgtgttt caaacccggc agcttagttg
      901 ccgttcttcc gaatagcatc ggtaacatga gcaaagtctg ccgccttaca acggctctcc
      961 cgctgacgcc gtcccggact gatgggctgc ctgtatcgag tggtgatttt gtgccgagct
     1021 cattactcgc atccattctc aggctgtctc gtctcgtctc aggtctcata ctggggccac
     1081 catgaggctg attgcgattt cgctttgttg tttgatgccg tgcacggtgc gatgccgatc
     1141 ttggcgcaat atcgagcctc ttcacggctg gaatgagaat gacacttccg gtactatttg
     1201 gagaatggag ttcaatccgc aaatgcgaga ccaccaggat acatagatta ccacaactcc
     1261 gagcccttcc acccacagaa tcaggggata acgcaggaaa gaacatgtga gcaaaaggcc
     1321 agcaaaaggc caggaaccgt aaaaaggccg cgttgctggc gtttttccat aggctccgcc
     1381 cccctgacga gcatcacaaa aatcgacgct caagtcagag gtggcgaaac ccgacaggac
     1441 tataaagata ccaggcgttt ccccctggaa gctccctcgt gcgctctcct gttccgaccc
     1501 tgccgcttac cggatacctg tccgcctttc tcccttcggg aagcgtggcg ctttctcata
     1561 gctcacgctg taggtatctc agttcggtgt aggtcgttcg ctccaagctg ggctgtgtgc
     1621 acgaaccccc cgttcagccc gaccgctgcg ccttatccgg taactatcgt cttgagtcca
     1681 acccggtaag acacgactta tcgccactgg cagcagccac tggtaacagg attagcagag
     1741 cgaggtatgt aggcggtgct acagagttct tgaagtggtg gcctaactac ggctacacta
     1801 gaagaacagt atttggtatc tgcgctctgc tgaagccagt taccttcgga aaaagagttg
     1861 gtagctcttg atccggcaaa caaaccaccg ctggtagcgg tggttttttt gtttgcaagc
     1921 agcagattac gcgcagaaaa aaaggatctc aagaagatcc tttgatcttt tctacggggt
     1981 ctgacgctca gtggaacgaa aactcacgtt aagggatttt ggtcatgcat tctaggtgat
     2041 tattatttgc cgactacctt ggtgatctcg cctttcacgt agtggacaaa ttcttccaac
     2101 tgatctgcgc gcgaggccaa gcgatcttct tcttgtccaa gataagcctg tctagcttca
     2161 agtatgacgg gctgatactg ggccggcagg cgctccattg cccagtcgg
//
