LOCUS       sequence-410914-        3012 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Level 0 plasmid containing a terminator sequence from gene 49202 
            from Phaeodactylum tricornutum with EF overhangs used to build a 
            level 1 construct..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3012)
  AUTHORS   Pollak B, Matute T, Nunez I, Cerda A, Lopez C, Vargas V, Kan A, 
            Bielinski V, von Dassow P, Dupont CL, Federici F
  TITLE     Universal loop assembly: open, efficient and cross-kingdom DNA 
            fabrication.
  JOURNAL   Synth Biol (Oxf). 2020;5(1):ysaa001. doi: 10.1093/synbio/ysaa001. 
            Epub 2020 Feb 5.
  PUBMED    32161816
REFERENCE   2  (bases 1 to 3012)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Synth Biol 
            (Oxf)."; date: "2020"; volume: "5(1):ysaa001. doi"; pages: " 
            10.1093/synbio/ysaa001. Epub 2020 Feb 5"
COMMENT     Sequence Label: pL0_EF-P49
FEATURES             Location/Qualifiers
     source          1..3012
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      complement(2160..2748)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(2240..2259)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(2839..3012,1..618))
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
ORIGIN
        1 atccttcggc gcgattttgc cggttactgc gctgtaccaa atgcgggaca acgtaagcac
       61 tacatttcgc tcatcgccag cccagtcggg cggcgagttc catagcgtta aggtttcatt
      121 tagcgcctca aatagatcct gttcaggaac cggatcaaag agttcctccg ccgctggacc
      181 taccaaggca acgctatgtt ctcttgcttt tgtcagcaag atagccagat caatgtcgat
      241 cgtggctggc tcgaagatac cagcaagaat gtcattgcgc tgccattctc caaattgcag
      301 ttcgcgctta gctggataac gccacggaat gatgtcgtcg tgcacaacaa tggtgacttc
      361 tacagcgcgg agaatctcac tctctccagg ggaagccgaa gtttccaaaa ggtcgttgat
      421 caaagctcgc cgcgttgttt catcaagcct tacggtcacc gtaaccagca aatcaatatc
      481 actgtgtggc ttcaggccgc catccactgc ggagccgtac aaatgtacgg ccagcaacgt
      541 cggttcgaga tggcgctcga tgacgccaac tacctctgat agttgagtcg atacttcggc
      601 gatcaccgct tccctcatgc gaaacgatcc tcatcctgtc tcttgatcag atattgatcc
      661 cctgcgccat cagatccttg gcggcaagaa agccatccag tttactttgc agggcttccc
      721 aaccttacca gagggcgccc cagctggcaa ttccggttcg cttgctaagc ttgcatgcct
      781 gcaagtcgac tctagaggag cggtgatcac aggcagcaac gctctgtcat cgttacaatc
      841 aacatgctac cctccgcgag atcatccgtg tttcaaaccc ggcagcttag ttgccgttct
      901 tccgaatagc atcggtaaca tgagcaaagt ctgccgcctt acaacggctc tcccgctgac
      961 gccgtcccgg actgatgggc tgcctgtatc gagtggtgat tttgtgccga gctcattact
     1021 cgcatccatt ctcaggctgt ctcgtctcgt ctcaggtctc agcttttcga tcgacgagct
     1081 taccttcacg gccaccgttc ccgaccccga cgttgttcag gcgatcatta cgggtggcag
     1141 cgccgctccg ggagccaccg gtttgtacgg cattgccacc gcagatgccg agtgcccggc
     1201 caacgagtgc atccatgtgg acgtgattgt gtacgccatt ttcgcgaatg aagccacgac
     1261 caacttgatt gtggaaatca gcggcgacgc ggtcatgtct gtgggacgta ctgcccgcaa
     1321 gttgcgtgcg aaagtccagc ccacacgcga gttgcaggag acgctccaga tgcaagcctt
     1381 tcgtaacgag attgtcctcc cccctttgcc tggttcgggg gccacgttgg ccaacgtgtg
     1441 cgcggcggcg gtgcccttcg ccgtggccgt ggtggccggt gcgctcgctc tttaaaaagt
     1501 gagagtttgt gtgtgtgaaa gacgcgtcgc gagacgctcc ttatttccgt cggcatgtgt
     1561 cttgtcgtgg tcctatgtga tccccgggtc ggttcacggg agttgccgaa gtagagaata
     1621 agaattatat aactgtacag agatacagac atgtgctgct attacgtcca acagtaggta
     1681 tacacctccc gggcaataga gtgggccaca tccatacaca cacacccgag ttggtagatt
     1741 gatttcggtt cgtcgaatgc caagaatgtg cttcctgaag aaaatttgct ccttcccact
     1801 ccgtccgtac gaccatccgc ccgtgtacag cgcggcagaa gtagaaacga gtaccaaggc
     1861 tctgtgtgtg tgtgtgtacc gtttggacgc gtcggcaaag gtacggcagt cctccgggtg
     1921 tgtcccgtac tcgtggtacc aacgaatctg cacaattgtg ccgttcggag gacacgcctt
     1981 cccggatgca tgcatccgga agaccgtcga tgcgattcgc ttgagaccac caggatacat
     2041 agattaccac aactccgagc ccttccaccc acagaatcag gggataacgc aggaaagaac
     2101 atgtgagcaa aaggccagca aaaggccagg aaccgtaaaa aggccgcgtt gctggcgttt
     2161 ttccataggc tccgcccccc tgacgagcat cacaaaaatc gacgctcaag tcagaggtgg
     2221 cgaaacccga caggactata aagataccag gcgtttcccc ctggaagctc cctcgtgcgc
     2281 tctcctgttc cgaccctgcc gcttaccgga tacctgtccg cctttctccc ttcgggaagc
     2341 gtggcgcttt ctcatagctc acgctgtagg tatctcagtt cggtgtaggt cgttcgctcc
     2401 aagctgggct gtgtgcacga accccccgtt cagcccgacc gctgcgcctt atccggtaac
     2461 tatcgtcttg agtccaaccc ggtaagacac gacttatcgc cactggcagc agccactggt
     2521 aacaggatta gcagagcgag gtatgtaggc ggtgctacag agttcttgaa gtggtggcct
     2581 aactacggct acactagaag aacagtattt ggtatctgcg ctctgctgaa gccagttacc
     2641 ttcggaaaaa gagttggtag ctcttgatcc ggcaaacaaa ccaccgctgg tagcggtggt
     2701 ttttttgttt gcaagcagca gattacgcgc agaaaaaaag gatctcaaga agatcctttg
     2761 atcttttcta cggggtctga cgctcagtgg aacgaaaact cacgttaagg gattttggtc
     2821 atgcattcta ggtgattatt atttgccgac taccttggtg atctcgcctt tcacgtagtg
     2881 gacaaattct tccaactgat ctgcgcgcga ggccaagcga tcttcttctt gtccaagata
     2941 agcctgtcta gcttcaagta tgacgggctg atactgggcc ggcaggcgct ccattgccca
     3001 gtcggcagcg ac
//
