LOCUS       sequence-410916-        2573 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  MoClo lvl0 3'UTR+Terminator part.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2573)
  AUTHORS   Yang EJY, Nemhauser JL
  TITLE     Building a pipeline to identify and engineer constitutive and 
            repressible promoters.
  JOURNAL   Quant Plant Biol. 2023 Oct 19;4:e12. doi: 10.1017/qpb.2023.10. 
            eCollection 2023.
  PUBMED    37901686
REFERENCE   2  (bases 1 to 2573)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Quant Plant
            Biol."; date: "2023-10-19"; volume: "4:e12. doi"; pages: " 
            10.1017/qpb.2023.10. eCollection 2023"
COMMENT     Sequence Label: pICH41276(3Uter)-tAT4G16845
FEATURES             Location/Qualifiers
     source          1..2573
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      271..859
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     760..779
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     1013..1030
                     /label=L4440
                     /note="L4440 vector, forward primer"
     primer_bind     complement(1470..1488)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        1556..1660
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(1882..2573,1..100)
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
ORIGIN
        1 tcttggacaa gaagaagatc gcttggcctc gcgcgcagat cagttggaag aatttgtcca
       61 ttacgtaaaa ggcgagatca ccaaggtagt cggcaaataa ctgtcagacc aagtttactc
      121 atatatactt tagattgatt taaaacttca tttttaattt aaaaggatct aggtgaagat
      181 cctttttgat aatctcatga ccaaaatccc ttaacgtgag ttttcgttcc actgagcgtc
      241 agaccccgta gaaaagatca aaggatcttc ttgagatcct ttttttctgc gcgtaatctg
      301 ctgcttgcaa acaaaaaaac caccgctacc agcggtggtt tgtttgccgg atcaagagct
      361 accaactctt tttccgaagg taactggctt cagcagagcg cagataccaa atactgttct
      421 tctagtgtag ccgtagttag gccaccactt caagaactct gtagcaccgc ctacatacct
      481 cgctctgcta atcctgttac cagtggctgc tgccagtggc gataagtcgt gtcttaccgg
      541 gttggactca agacgatagt taccggataa ggcgcagcgg tcgggctgaa cggggggttc
      601 gtgcacacag cccagcttgg agcgaacgac ctacaccgaa ctgagatacc tacagcgtga
      661 gctatgagaa agcgccacgc ttcccgaagg gagaaaggcg gacaggtatc cggtaagcgg
      721 cagggtcgga acaggagagc gcacgaggga gcttccaggg ggaaacgcct ggtatcttta
      781 tagtcctgtc gggtttcgcc acctctgact tgagcgtcga tttttgtgat gctcgtcagg
      841 ggggcggagc ctatggaaaa acgccagcaa cgcggccttt ttacggttcc tggccttttg
      901 ctggcctttt gctcacatgt tctttcctgc gttatcccct gattctgtgg ataaccgtat
      961 taccgccttt gagtgagctg ataccgctcg ccgcagccga acgaccgagc gcagcgagtc
     1021 agtgagcgag gaagcggaag agcgcccaat acgcaaaccg cctctccccg cgcgttggcc
     1081 gattcattaa tcactctgtg gtctcagctt ttaaatagga aacactccgg tttagatgat
     1141 accgatctat cggattgtaa cttattcttc tttcttaaaa aaattgttta ggagcaaaca
     1201 aagattttat ttgttagtgt attcaactga ttacattttt agttaaaaaa atggattctc
     1261 cttaataact aaagactgaa aaataagata agtttcctta atttttcttt ttgacttgag
     1321 aaaaagctcc tctagacctc tagtaaatag gagttatata ttaatcaagt acataacata
     1381 aaaatatata tattaagtgc aaatagattg aaaacaaatc aagaaattaa cgcttgagac
     1441 cacgaagtgg ctcttcagtg gacgaaaggg cctcgtgata cgcctatttt tataggttaa
     1501 tgtcatgata ataatggttt cttagacgtc aggtggcact tttcggggaa atgtgcgcgg
     1561 aacccctatt tgtttatttt tctaaataca ttcaaatatg tatccgctca tgagacaata
     1621 accctgataa atgcttcaat aatattgaaa aaggaagagt atgcgctcac gcaactggtc
     1681 cagaaccttg accgaacgca gcggtggtaa cggcgcagtg gcggttttca tggcttgtta
     1741 tgactgtttt tttttggggt acagtctatg cctcgggcat ccaagcagca agcgcgttac
     1801 gccgtgggtc gatgtttgat gttatggagc agcaacgatg ttacgcagca gggcagtcgc
     1861 cctaaaacaa agttaaacat catgagggaa gcggtgatcg ccgaagtatc gactcaacta
     1921 tcagaggtag ttggcgtcat cgagcgccat ctcgaaccga cgttgctggc cgtacatttg
     1981 tacggctccg cagtggatgg cggcctgaag ccacacagcg atattgattt gctggttacg
     2041 gtgaccgtaa ggcttgatga aacaacgcgg cgagctttga tcaacgacct tttggaaact
     2101 tcggcttccc ctggagagag cgagattctc cgcgctgtag aagtcaccat tgttgtgcac
     2161 gacgacatca ttccgtggcg ttatccagct aagcgcgaac tgcaatttgg agaatggcag
     2221 cgcaatgaca ttcttgcagg tatcttcgag ccagccacga tcgacattga tctggctatc
     2281 ttgctgacaa aagcaagaga acatagcgtt gccttggtag gtccagcggc ggaggaactc
     2341 tttgatccgg ttcctgaaca ggatctattt gaggcgctaa atgaaacctt aacgctatgg
     2401 aactcgccgc ccgactgggc tggcgatgag cgaaatgtag tgcttacgtt gtcccgcatt
     2461 tggtacagcg cagtaaccgg caaaatcgcg ccgaaggatg tcgctgccga ctgggcaatg
     2521 gagcgcctgc cggcccagta tcagcccgtc atacttgaag ctagacaggc tta
//
