LOCUS       sequence-410918-        9648 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  expression of mutant G protein alpha 12 in mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9648)
  TITLE     Mariotti lab plasmids
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 9648)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: psd44-G12QL
FEATURES             Location/Qualifiers
     source          1..9648
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        1..511
                     /label=hPGK promoter
                     /note="human phosphoglycerate kinase 1 promoter"
     primer_bind     430..448
                     /label=hPGK-F
                     /note="Human PGK promoter, forward primer"
     misc_feature    1744..2332
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(1797..1817)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(2215..2226)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             2419..2652
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    2724..2845
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(2761..2780)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     2815..2834
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      2885..3020
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(3040..3059)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(3041..3059)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(3069..3086)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(3069..3085)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(3078..3100)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      3227..3682
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(3314..3333)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     3524..3545
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        3708..3812
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(3813..3881,3882..4673)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(4031..4050)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      4844..5432
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     5333..5352
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     5586..5603
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    5720..5741
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(5756..5761,5762..5779,5780..5786)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     mobile_element  5787..6554
                     /mobile_element_type="insertion sequence:IS1"
                     /label=IS1
                     /note="prokaryotic transposable element"
     protein_bind    6571..6587
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     6576..6598
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     6595..6611
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     6595..6611
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     promoter        6677..6903
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             6904..7084
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    7131..7256
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    7749..7982
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             8167..8211
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    8478..8595
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     primer_bind     complement(8607..8627)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        8612..8941
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      8792..8927
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             8951..9550
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(8951..8970)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     9447..9467
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
ORIGIN
        1 ggggttgggg ttgcgccttt tccaaggcag ccctgggttt gcgcagggac gcggctgctc
       61 tgggcgtggt tccgggaaac gcagcggcgc cgaccctggg tctcgcacat tcttcacgtc
      121 cgttcgcagc gtcacccgga tcttcgccgc tacccttgtg ggccccccgg cgacgcttcc
      181 tgctccgccc ctaagtcggg aaggttcctt gcggttcgcg gcgtgccgga cgtgacaaac
      241 ggaagccgca cgtctcacta gtaccctcgc agacggacag cgccagggag caatggcagc
      301 gcgccgaccg cgatgggctg tggccaatag cggctgctca gcagggcgcg ccgagagcag
      361 cggccgggaa ggggcggtgc gggaggcggg gtgtggggcg gtagtgtggg ccctgttcct
      421 gcccgcgcgg tgttccgcat tctgcaagcc tccggagcgc acgtcggcag tcggctccct
      481 cgttgaccga atcaccgacc tctctcccca gggggatccc gggcccgcgg taccaccatg
      541 tccggggtgg tgcggaccct cagccgctgc ctgctgccgg ccgaggccgg cggggcccgc
      601 gagcgcaggg cgggcagcgg cgcgcgcgac gcggagcgcg aggcccggag gcgtagccgc
      661 gacatcgacg cgctgctggc ccgcgagcgg cgcgcggtcc ggcgcctggt gaagatcctg
      721 ctgctgggcg cgggcgagag cggcaagtcc acgttcctca agcagatgcg catcatccac
      781 ggccgcgagt tcgaccagaa ggcgctgctg gagttccgcg acaccatctt cgacaacatc
      841 ctcaagggct caagggttct tgttgatgca cgagataagc ttggcattcc ttggcagtat
      901 tctgaaaatg agaagcatgg gatgttcctg atggccttcg agaacaaggc ggggctgcct
      961 gtggagccgg ccaccttcca gctgtacgtc ccggccctga gcgcactctg gagggattct
     1021 ggcatcaggg aggctttcag ccggagaagc gagtttcagc tgggggagtc ggtgaagtac
     1081 ttcctggaca acttggaccg gatcggccag ctgaattact ttcctagtaa gcaagatatc
     1141 ctgctggcta ggaaagccac caagggaatt gtggagcatg acttcgttat taagaagatc
     1201 ccctttaaga tggtggatgt gggcggcctg cggtcccagc gccagaagtg gttccagtgc
     1261 ttcgacggga tcacgtccat cctgttcatg gtctcctcca gcgagtacga ccaggtcctc
     1321 atggaggaca ggcgcaccaa ccggctggtg gagtccatga acatcttcga gaccatcgtc
     1381 aacaacaagc tcttcttcaa cgtctccatc attctcttcc tcaacaagat ggacctcctg
     1441 gtggagaagg tgaagaccgt gagcatcaag aagcacttcc cggacttcag gggcgacccg
     1501 cacaggctgg aggacgtcca gcgctacctg gtccagtgct tcgacaggaa gagacggaac
     1561 cgcagcaagc cactcttcca ccacttcacc accgccatcg acaccgagaa cgtccgcttc
     1621 gtgttccatg ctgtgaaaga caccatcctg caggagaacc tgaaggacat catgctgcag
     1681 tgactcgaga tctgagtccg gtagcgctag ctgatcaccc gggggccggc ctgtacagtc
     1741 gacaatcaac ctctggatta caaaatttgt gaaagattga ctggtattct taactatgtt
     1801 gctcctttta cgctatgtgg atacgctgct ttaatgcctt tgtatcatgc tattgcttcc
     1861 cgtatggctt tcattttctc ctccttgtat aaatcctggt tgctgtctct ttatgaggag
     1921 ttgtggcccg ttgtcaggca acgtggcgtg gtgtgcactg tgtttgctga cgcaaccccc
     1981 actggttggg gcattgccac cacctgtcag ctcctttccg ggactttcgc tttccccctc
     2041 cctattgcca cggcggaact catcgccgcc tgccttgccc gctgctggac aggggctcgg
     2101 ctgttgggca ctgacaattc cgtggtgttg tcggggaagc tgacgtcctt tccatggctg
     2161 ctcgcctgtg ttgccacctg gattctgcgc gggacgtcct tctgctacgt cccttcggcc
     2221 ctcaatccag cggaccttcc ttcccgcggc ctgctgccgg ctctgcggcc tcttccgcgt
     2281 cttcgccttc gccctcagac gagtcggatc tccctttggg ccgcctcccc gcctggaatt
     2341 cgagctcggt acctttaaga ccaatgactt acaaggcagc tgtagatctt agccactttt
     2401 taaaagaaaa ggggggactg gaagggctaa ttcactccca acgaagacaa gatctgcttt
     2461 ttgcttgtac tgggtctctc tggttagacc agatctgagc ctgggagctc tctggctaac
     2521 tagggaaccc actgcttaag cctcaataaa gcttgccttg agtgcttcaa gtagtgtgtg
     2581 cccgtctgtt gtgtgactct ggtaactaga gatccctcag acccttttag tcagtgtgga
     2641 aaatctctag cagtagtagt tcatgtcatc ttattattca gtatttataa cttgcaaaga
     2701 aatgaatatc agagagtgag aggaacttgt ttattgcagc ttataatggt tacaaataaa
     2761 gcaatagcat cacaaatttc acaaataaag catttttttc actgcattct agttgtggtt
     2821 tgtccaaact catcaatgta tcttatcatg tctggctcta gctatcccgc ccctaactcc
     2881 gcccatcccg cccctaactc cgcccagttc cgcccattct ccgccccatg gctgactaat
     2941 tttttttatt tatgcagagg ccgaggccgc ctcggcctct gagctattcc agaagtagtg
     3001 aggaggcttt tttggaggcc tagggacgta cccaattcgc cctatagtga gtcgtattac
     3061 gcgcgctcac tggccgtcgt tttacaacgt cgtgactggg aaaaccctgg cgttacccaa
     3121 cttaatcgcc ttgcagcaca tccccctttc gccagctggc gtaatagcga agaggcccgc
     3181 accgatcgcc cttcccaaca gttgcgcagc ctgaatggcg aatgggacgc gccctgtagc
     3241 ggcgcattaa gcgcggcggg tgtggtggtt acgcgcagcg tgaccgctac acttgccagc
     3301 gccctagcgc ccgctccttt cgctttcttc ccttcctttc tcgccacgtt cgccggcttt
     3361 ccccgtcaag ctctaaatcg ggggctccct ttagggttcc gatttagtgc attacggcac
     3421 ctcgacccca aaaaacttga ttagggtgat ggttcacgta gtgggccatc gccctgatag
     3481 acggtttttc gccctttgac gttggagtcc acgttcttta atagtggact cttgttccaa
     3541 actggaacaa cactcaaccc tatctcggtc tattcttttg atttataagg gattttgccg
     3601 atttcggcct attggttaaa aaatgagctg atttaacaaa aatttaacgc gaattttaac
     3661 aaaatattaa cgcttacaat ttaggtggca cttttcgggg aaatgtgcgc ggaaccccta
     3721 tttgtttatt tttctaaata cattcaaata tgtatccgct catgagacaa taaccctgat
     3781 aaatgcttca ataatattga aaaaggaaga gtatgagtat tcaacatttc cgtgtcgccc
     3841 ttattccctt ttttgcggca ttttgccttc ctgtttttgc tcacccagaa acgctggtga
     3901 aagtaaaaga tgctgaagat cagttgggtg cacgagtggg ttacatcgaa ctggatctca
     3961 acagcggtaa gatccttgag agttttcgcc ccgaagaacg ttttccaatg atgagcactt
     4021 ttaaagttct gctatgtggc gcggtattat cccgtattga cgccgggcaa gagcaactcg
     4081 gtcgccgcat acactattct cagaatgact tggttgagta ctcaccagtc acagaaaagc
     4141 atcttacgga tggcatgaca gtaagagaat tatgcagtgc tgccataacc atgagtgata
     4201 acactgcggc caacttactt ctgacaacga tcggaggacc gaaggagcta accgcttttt
     4261 tgcacaacat gggggatcat gtaactcgcc ttgatcgttg ggaaccggag ctgaatgaag
     4321 ccataccaaa cgacgagcgt gacaccacga tgcctgtagc aatggcaaca acgttgcgca
     4381 aactattaac tggcgaacta cttactctag cttcccggca acaattaata gactggatgg
     4441 aggcggataa agttgcagga ccacttctgc gctcggccct tccggctggc tggtttattg
     4501 ctgataaatc tggagccggt gagcgtgggt ctcgcggtat cattgcagca ctggggccag
     4561 atggtaagcc ctcccgtatc gtagttatct acacgacggg gagtcaggca actatggatg
     4621 aacgaaatag acagatcgct gagataggtg cctcactgat taagcattgg taactgtcag
     4681 accaagttta ctcatatata ctttagattg atttaaaact tcatttttaa tttaaaagga
     4741 tctaggtgaa gatccttttt gataatctca tgaccaaaat cccttaacgt gagttttcgt
     4801 tccactgagc gtcagacccc gtagaaaaga tcaaaggatc ttcttgagat cctttttttc
     4861 tgcgcgtaat ctgctgcttg caaacaaaaa aaccaccgct accagcggtg gtttgtttgc
     4921 cggatcaaga gctaccaact ctttttccga aggtaactgg cttcagcaga gcgcagatac
     4981 caaatactgt tcttctagtg tagccgtagt taggccacca cttcaagaac tctgtagcac
     5041 cgcctacata cctcgctctg ctaatcctgt taccagtggc tgctgccagt ggcgataagt
     5101 cgtgtcttac cgggttggac tcaagacgat agttaccgga taaggcgcag cggtcgggct
     5161 gaacgggggg ttcgtgcaca cagcccagct tggagcgaac gacctacacc gaactgagat
     5221 acctacagcg tgagctatga gaaagcgcca cgcttcccga agggagaaag gcggacaggt
     5281 atccggtaag cggcagggtc ggaacaggag agcgcacgag ggagcttcca gggggaaacg
     5341 cctggtatct ttatagtcct gtcgggtttc gccacctctg acttgagcgt cgatttttgt
     5401 gatgctcgtc aggggggcgg agcctatgga aaaacgccag caacgcggcc tttttacggt
     5461 tcctggcctt ttgctggcct tttgctcaca tgttctttcc tgcgttatcc cctgattctg
     5521 tggataaccg tattaccgcc tttgagtgag ctgataccgc tcgccgcagc cgaacgaccg
     5581 agcgcagcga gtcagtgagc gaggaagcgg aagagcgccc aatacgcaaa ccgcctctcc
     5641 ccgcgcgttg gccgattcat taatgcagct ggcacgacag gtttcccgac tggaaagcgg
     5701 gcagtgagcg caacgcaatt aatgtgagtt agctcactca ttaggcaccc caggctttac
     5761 actttatgct tccggctcgt atgttgggta atgactccaa cttattgata gtgttttatg
     5821 ttcagataat gcccgatgac tttgtcatgc agctccaccg attttgagaa cgacagcgac
     5881 ttccgtccca gccgtgccag gtgctgcctc agattcaggt tatgccgctc aattcgctgc
     5941 gtatatcgct tgctgattac gtgcagcttt cccttcaggc gggattcata cagcggccag
     6001 ccatccgtca tccatatcac cacgtcaaag ggtgacagca ggctcataag acgccccagc
     6061 gtcgccatag tgcgttcacc gaatacgtgc gcaacaaccg tcttccggag actgtcatac
     6121 gcgtaaaaca gccagcgctg gcgcgattta gccccgacat agccccactg ttcgtccatt
     6181 tccgcgcaga cgatgacgtc actgcccggc tgtatgcgcg aggttaccga ctgcggcctg
     6241 agttttttaa gtgacgtaaa atcgtgttga ggccaacgcc cataatgcgg gctgttgccc
     6301 ggcatccaac gccattcatg gccatatcaa tgattttctg gtgcgtaccg ggttgagaag
     6361 cggtgtaagt gaactgcagt tgccatgttt tacggcagtg agagcagaga tagcgctgat
     6421 gtccggcggt gcttttgccg ttacgcacca ccccgtcagt agctgaacag gagggacagc
     6481 tgatagaaac agaagccact ggagcacctc aaaaacacca tcatacacta aatcagtaag
     6541 ttggcagcat cacccgtatg ttgtgtggaa ttgtgagcgg ataacaattt cacacaggaa
     6601 acagctatga ccatgattac gccaagcgcg cgtttaaact cactaaaggg aacaaaagct
     6661 ggagctgcaa gcttaatgta gtcttatgca atactcttgt agtcttgcaa catggtaacg
     6721 atgagttagc aacatgcctt acaaggagag aaaaagcacc gtgcatgccg attggtggaa
     6781 gtaaggtggt acgatcgtgc cttattagga aggcaacaga cgggtctgac atggattgga
     6841 cgaaccactg aattgccgca ttgcagagat attgtattta agtgcctagc tcgatacata
     6901 aacgggtctc tctggttaga ccagatctga gcctgggagc tctctggcta actagggaac
     6961 ccactgctta agcctcaata aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg
     7021 ttgtgtgact ctggtaacta gagatccctc agaccctttt agtcagtgtg gaaaatctct
     7081 agcagtggcg cccgaacagg gacttgaaag cgaaagggaa accagaggag ctctctcgac
     7141 gcaggactcg gcttgctgaa gcgcgcacgg caagaggcga ggggcggcga ctggtgagta
     7201 cgccaaaaat tttgactagc ggaggctaga aggagagaga tgggtgcgag agcgtcagta
     7261 ttaagcgggg gagaattaga tcgcgatggg aaaaaattcg gttaaggcca gggggaaaga
     7321 aaaaatataa attaaaacat atagtatggg caagcaggga gctagaacga ttcgcagtta
     7381 atcctggcct gttagaaaca tcagaaggct gtagacaaat actgggacag ctacaaccat
     7441 cccttcagac aggatcagaa gaacttagat cattatataa tacagtagca accctctatt
     7501 gtgtgcatca aaggatagag ataaaagaca ccaaggaagc tttagacaag atagaggaag
     7561 agcaaaacaa aagtaagacc accgcacagc aagcggccgc tgatcttcag acctggagga
     7621 ggagatatga gggacaattg gagaagtgaa ttatataaat ataaagtagt aaaaattgaa
     7681 ccattaggag tagcacccac caaggcaaag agaagagtgg tgcagagaga aaaaagagca
     7741 gtgggaatag gagctttgtt ccttgggttc ttgggagcag caggaagcac tatgggcgca
     7801 gcgtcaatga cgctgacggt acaggccaga caattattgt ctggtatagt gcagcagcag
     7861 aacaatttgc tgagggctat tgaggcgcaa cagcatctgt tgcaactcac agtctggggc
     7921 atcaagcagc tccaggcaag aatcctggct gtggaaagat acctaaagga tcaacagctc
     7981 ctggggattt ggggttgctc tggaaaactc atttgcacca ctgctgtgcc ttggaatgct
     8041 agttggagta ataaatctct ggaacagatt tggaatcaca cgacctggat ggagtgggac
     8101 agagaaatta acaattacac aagcttaata cactccttaa ttgaagaatc gcaaaaccag
     8161 caagaaaaga atgaacaaga attattggaa ttagataaat gggcaagttt gtggaattgg
     8221 tttaacataa caaattggct gtggtatata aaattattca taatgatagt aggaggcttg
     8281 gtaggtttaa gaatagtttt tgctgtactt tctatagtga atagagttag gcagggatat
     8341 tcaccattat cgtttcagac ccacctccca accccgaggg gacccgacag gcccgaagga
     8401 atagaagaag aaggtggaga gagagacaga gacagatcca ttcgattagt gaacggatct
     8461 cgacggtatc ggttaacttt taaaagaaaa ggggggattg gggggtacag tgcaggggaa
     8521 agaatagtag acataatagc aacagacata caaactaaag aattacaaaa acaaattaca
     8581 aaaattcaaa attttatcga ccctgtggaa tgtgtgtcag ttagggtgtg gaaagtcccc
     8641 aggctcccca gcaggcagaa gtatgcaaag catgcatctc aattagtcag caaccaggtg
     8701 tggaaagtcc ccaggctccc cagcaggcag aagtatgcaa agcatgcatc tcaattagtc
     8761 agcaaccata gtcccgcccc taactccgcc catcccgccc ctaactccgc ccagttccgc
     8821 ccattctccg ccccatggct gactaatttt ttttatttat gcagaggccg aggccgcctc
     8881 ggcctctgag ctattccaga agtagtgagg aggctttttt ggaggcctag gcttttgcaa
     8941 aaagcttacc atgaccgagt acaagcccac ggtgcgcctc gccacccgcg acgacgtccc
     9001 cagggccgta cgcaccctcg ccgccgcgtt cgccgactac cccgccacgc gccacaccgt
     9061 cgatccggac cgccacatcg agcgggtcac cgagctgcaa gaactcttcc tcacgcgcgt
     9121 cgggctcgac atcggcaagg tgtgggtcgc ggacgacggc gccgcggtgg cggtctggac
     9181 cacgccggag agcgtcgaag cgggggcggt gttcgccgag atcggcccgc gcatggccga
     9241 gttgagcggt tcccggctgg ccgcgcagca acagatggaa ggcctcctgg cgccgcaccg
     9301 gcccaaggag cccgcgtggt tcctggccac cgtcggcgtc tcgcccgacc accagggcaa
     9361 gggtctgggc agcgccgtcg tgctccccgg agtggaggcg gccgagcgcg ccggggtgcc
     9421 cgccttcctg gagacctccg cgccccgcaa cctccccttc tacgagcggc tcggcttcac
     9481 cgtcaccgcc gacgtcgagg tgcccgaagg accgcgcacc tggtgcatga cccgcaagcc
     9541 cggtgcctga cgcccgcccc acgacccgca gcgcccgacc gaaaggagcg cacgacccca
     9601 tgcatcgatc acgagactag cctcgagaag cttgatatcg aattccac
//
