LOCUS       sequence-410934-        9194 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  expressing SARS-CoV-2 BA.2.86 spike protein for pseudovirus 
            production.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9194)
  TITLE     SARS-CoV-2 variants
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 9194)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: pcDNA3.3_SARS2_BA.2.86
FEATURES             Location/Qualifiers
     source          1..9194
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             complement(join(209..1000,1001..1069))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     832..851
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(1070..1174)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(1249..1268)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        1440..1819
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        1820..2023
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     1974..1994
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     2020..2044
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     primer_bind     complement(5970..5989)
                     /label=TK-pA-R
                     /note="Thymidine kinase polyA, reverse primer"
     polyA_signal    6014..6062
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase 
                     polyadenylation signal (Cole and Stacy, 1985)"
     rep_origin      6264..6692
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(6351..6370)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     6561..6582
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(6701..6721)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        6706..7035
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      6886..7021
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     6948..6967
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     CDS             7102..7896
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(7156..7175)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     7766..7785
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     polyA_signal    8072..8193
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(8109..8128)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     8163..8182
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(8242..8258)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(8242..8258)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(8255..8277)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    8266..8282
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(8290..8296,8297..8314,8315..8320))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    8335..8356
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(8473..8490)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(join(8644..9194,1..38))
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(8724..8743)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 gcaagcagca gattacgcgc agaaaaaaag gatctcaaga agatcctttg atcttttcta
       61 cggggtctga cgctcagtgg aacgaaaact cacgttaagg gattttggtc atgagattat
      121 caaaaaggat cttcacctag atccttttaa attaaaaatg aagttttaaa tcaatctaaa
      181 gtatatatga gtaaacttgg tctgacagtt accaatgctt aatcagtgag gcacctatct
      241 cagcgatctg tctatttcgt tcatccatag ttgcctgact ccccgtcgtg tagataacta
      301 cgatacggga gggcttacca tctggcccca gtgctgcaat gataccgcga gacccacgct
      361 caccggctcc agatttatca gcaataaacc agccagccgg aagggccgag cgcagaagtg
      421 gtcctgcaac tttatccgcc tccatccagt ctattaattg ttgccgggaa gctagagtaa
      481 gtagttcgcc agttaatagt ttgcgcaacg ttgttgccat tgctacaggc atcgtggtgt
      541 cacgctcgtc gtttggtatg gcttcattca gctccggttc ccaacgatca aggcgagtta
      601 catgatcccc catgttgtgc aaaaaagcgg ttagctcctt cggtcctccg atcgttgtca
      661 gaagtaagtt ggccgcagtg ttatcactca tggttatggc agcactgcat aattctctta
      721 ctgtcatgcc atccgtaaga tgcttttctg tgactggtga gtactcaacc aagtcattct
      781 gagaatagtg tatgcggcga ccgagttgct cttgcccggc gtcaatacgg gataataccg
      841 cgccacatag cagaacttta aaagtgctca tcattggaaa acgttcttcg gggcgaaaac
      901 tctcaaggat cttaccgctg ttgagatcca gttcgatgta acccactcgt gcacccaact
      961 gatcttcagc atcttttact ttcaccagcg tttctgggtg agcaaaaaca ggaaggcaaa
     1021 atgccgcaaa aaagggaata agggcgacac ggaaatgttg aatactcata ctcttccttt
     1081 ttcaatatta ttgaagcatt tatcagggtt attgtctcat gagcggatac atatttgaat
     1141 gtatttagaa aaataaacaa ataggggttc cgcgcacatt tccccgaaaa gtgccacctg
     1201 acgtcgacgg atcgggagat ctcccgatcc cctatggtgc actctcagta caatctgctc
     1261 tgatgccgca tagttaagcc agtatctgct ccctgcttgt gtgttggagg tcgctgagta
     1321 gtgcgcgagc aaaatttaag ctacaacaag gcaaggcttg accgacaatt gcatgaagaa
     1381 tctgcttagg gttaggcgtt ttgcgctgct tcgcgatgta cgggccagat atacgcgttg
     1441 acattgatta ttgactagtt attaatagta atcaattacg gggtcattag ttcatagccc
     1501 atatatggag ttccgcgtta cataacttac ggtaaatggc ccgcctggct gaccgcccaa
     1561 cgacccccgc ccattgacgt caataatgac gtatgttccc atagtaacgc caatagggac
     1621 tttccattga cgtcaatggg tggagtattt acggtaaact gcccacttgg cagtacatca
     1681 agtgtatcat atgccaagta cgccccctat tgacgtcaat gacggtaaat ggcccgcctg
     1741 gcattatgcc cagtacatga ccttatggga ctttcctact tggcagtaca tctacgtatt
     1801 agtcatcgct attaccatgg tgatgcggtt ttggcagtac atcaatgggc gtggatagcg
     1861 gtttgactca cggggatttc caagtctcca ccccattgac gtcaatggga gtttgttttg
     1921 gcaccaaaat caacgggact ttccaaaatg tcgtaacaac tccgccccat tgacgcaaat
     1981 gggcggtagg cgtgtacggt gggaggtcta tataagcaga gctcgtttag tgaaccgtca
     2041 gatcgcctgg agacgccatc cacgctgttt tgacctccat agaagacacc gggaccgatc
     2101 cagcctccgg actgagctcc ttaaggttaa ctctagagaa ttcggatcca tgtttgtgtt
     2161 cctggtgctg ctgccactgg tgtccagcca gtgtgtgatg cctctgttca acctgatcac
     2221 cacgacccaa tcctacacca actccttcac caggggagtc tactaccctg acaaggtgtt
     2281 caggtcctct gtgctgcacc tcacccagga cctgttcctg ccattcttca gcaatgtgac
     2341 ctggttccat gccatctctg gcaccaatgg caccaagagg tttgacaacc ctgtgctgcc
     2401 attcaatgat ggagtctact ttgccagcac agagaagagc aacatcatca ggggctggat
     2461 ttttggcacc accctggaca gcaagaccca gtccctgctg attgtgaaca atgccaccaa
     2521 tgtgttcatt aaggtgtgtg agttccagtt ctgtaatgac ccattcctgg acgtctacca
     2581 caagaacaac aagtcctgga tggagtctga gagtggagtc tactcctctg ccaacaactg
     2641 tacctttgaa tatgtgagcc aaccattcct gatggacttg gagggcaagc agggcaactt
     2701 caagaacctg agggagtttg tgttcaagaa cattgatggc tacttcaaga tttacagcaa
     2761 acacacacca atcatcggta gggactttcc acagggcttc tctgccttgg aaccactggt
     2821 ggacctgcca attggcatca acatcaccag gttccagacc ctgctggctc tgaacaggtc
     2881 ctacctgaca cctggagact cctcctctgg ctggacagca ggagcagcag actactatgt
     2941 gggctacctc caaccaagga ccttcctgct gaaatacaat gagaatggca ccatcacaga
     3001 tgctgtggac tgtgccctgg acccactgtc tgagaccaag tgtaccctga aatccttcac
     3061 agtggagaag ggcatctacc agaccagcaa cttcagggtc caaccaacag agagcattgt
     3121 gaggtttcca aacgtcacca acctgtgtcc atttcacgag gtgttcaatg ccaccaggtt
     3181 tgcctctgtc tatgcctgga acaggacgag gattagcaac tgtgtggctg actactctgt
     3241 gctctacaac tttgccccct tcttcgcctt caagtgttat ggagtgagcc caaccaaact
     3301 gaatgacctg tgtttcacca atgtctatgc tgactccttt gtgattaagg gaaatgaggt
     3361 gagtcagatt gcccctggac aaacaggcaa cattgctgac tacaactaca aactgcctga
     3421 tgacttcaca ggctgtgtga ttgcctggaa cagcaacaag ctggacagca agcactcagg
     3481 caactacgac tactggtaca gactgttcag gaagagcaag ctgaaaccat ttgagaggga
     3541 catcagcaca gagatttacc aggctggcaa caaaccatgt aagggaaagg gcccgaactg
     3601 ttactttcca ctccagtcct atggcttcag accaacctat ggagtgggcc accaaccata
     3661 cagggtggtg gtgctgtcct ttgaactgct ccatgcccct gccacagtgt gtggaccaaa
     3721 gaagagcacc aacctggtga agaacaagtg tgtgaacttc aacttcaatg gactgacagg
     3781 cacaggagtg ctgacaaaga gcaacaagaa gttcctgcca ttccaacagt ttggcaggga
     3841 cattgttgac accacagatg ctgtgaggga cccacagacc ttggagattc tggacatcac
     3901 accatgttcc tttggaggag tgtctgtgat tacacctggc accaacacca gcaaccaggt
     3961 ggctgtgctc taccagggtg tgaactgtac tgaggtgtct gtggctatcc atgctgacca
     4021 acttacacca acctggaggg tctacagcac aggcagcaat gtgttccaga ccagggctgg
     4081 ctgtctgatt ggagcagagt atgtgaacaa ctcctatgag tgtgacatcc caattggagc
     4141 aggcgtctgt gcctcctacc agacccagac caagagccgt aggagggcaa ggtctgtggc
     4201 aagccagagc atcattgcct acacaatgag tctgggagca gagaactctg tggcttacag
     4261 caacaacagc attgccatcc caaccaactt caccatctct gtgaccacag aaattctgcc
     4321 tgtgagtatg accaagacct ctgtggactg tacaatgtat atctgtggag acagcacaga
     4381 gtgtagcaac ctgctgctcc aatatggctc cttctgtacc caacttaaga gggctctgac
     4441 aggcattgct gtggaacagg acaagaacac ccaggaggtg tttgcccagg tgaagcagat
     4501 ttacaagaca cctccaatca agtactttgg aggcttcaac ttcagccaga ttctgcctga
     4561 cccaagcaag ccaagcaaga ggtccttcat tgaggacctg ctgttcaaca aggtgaccct
     4621 ggctgatgct ggcttcatca agcaatatgg agactgtctg ggagacattg ctgcaaggga
     4681 cctgatttgt gcccagaagt tcaatggact gaccgtgctg cctccactgc tgacagatga
     4741 gatgattgcc caatacacct ctgccctgct ggctggcacc atcacctctg gctggacctt
     4801 tggagcagga gcagccctcc aaatcccatt tgctatgcag atggcttaca ggttcaatgg
     4861 cattggagtg acccagaatg tgctctatga gaaccagaaa ctgattgcca accagttcaa
     4921 ctctgccatt ggcaagattc aggactccct gttcagcaca gcctctgccc tgggcaaact
     4981 ccaagatgtg gtgaaccata atgcccaggc tctgaacacc ctggtgaagc aactttccag
     5041 caagtttgga gccatctcct ctgtgctgaa tgacatcctg agcagactgg acaaggtgga
     5101 ggctgaggtc cagattgaca gactgattac aggcagactc caatccctcc aaacctatgt
     5161 gacccaacaa cttatcaggg ctgctgagat tagggcatct gccaacctgg ctgccaccaa
     5221 gatgagtgag tgtgtgctgg gacaaagcaa gagggtggac ttctgtggca agggctacca
     5281 cctgatgagt tttccacagt ctgcccctca tggagtggtg ttcctgcatg tgacctatgt
     5341 gcctgcccag gagaagaact tcaccacagc ccctgccatc tgccatgatg gcaaggctca
     5401 ctttccaagg gagggagtgt ttgtgagcaa tggcacccac tggtttgtga cccagaggaa
     5461 cttctatgaa ccacagatta tcaccacaga caacaccttt gtgtctggca actgtgatgt
     5521 ggtgattggc attgtgaaca acacagtcta tgacccactc caacttgaac tggactcctt
     5581 caaggaggaa ctggacaaat acttcaagaa ccacaccagc cctgatgtgg acctgggaga
     5641 catctctggc atcaatgcct ctgtggtgaa catccagaag gagattgaca gactgaatga
     5701 ggtggctaag aacctgaatg agtccctgat tgacctccaa gaactgggca aatatgaaca
     5761 atacatcaag tggccatggt acatctggct gggcttcatt gctggactga ttgccattgt
     5821 gatggtgacc ataatgctgt gttgtatgac ctcctgttgt tcctgtctga aaggctgttg
     5881 ttcctgtggc tcctgttgta agtgactcga gccatggaag cttgatatct aactgactga
     5941 accggtggta ccaaacgggg gaggctaact gaaacacgga aggagacaat accggaagga
     6001 acccgcgcta tgacggcaat aaaaagacag aataaaacgc acgggtgttg ggtcgtttgt
     6061 tcataaacgc ggggttcggt cccagggctg gcactctgtc gataccccac cgagacccca
     6121 ttggggccaa tacgcccgcg tttcttcctt ttccccaccc caccccccaa gttcgggtga
     6181 aggcccaggg ctcgcagcca acgtcggggc ggcaggccct gccatagcag atctgcgcag
     6241 ctggggctct agggggtatc cccacgcgcc ctgtagcggc gcattaagcg cggcgggtgt
     6301 ggtggttacg cgcagcgtga ccgctacact tgccagcgcc ctagcgcccg ctcctttcgc
     6361 tttcttccct tcctttctcg ccacgttcgc cggctttccc cgtcaagctc taaatcgggg
     6421 gctcccttta gggttccgat ttagtgcttt acggcacctc gaccccaaaa aacttgatta
     6481 gggtgatggt tcacgtagtg ggccatcgcc ctgatagacg gtttttcgcc ctttgacgtt
     6541 ggagtccacg ttctttaata gtggactctt gttccaaact ggaacaacac tcaaccctat
     6601 ctcggtctat tcttttgatt tataagggat tttgccgatt tcggcctatt ggttaaaaaa
     6661 tgagctgatt taacaaaaat ttaacgcgaa ttaattctgt ggaatgtgtg tcagttaggg
     6721 tgtggaaagt ccccaggctc cccagcaggc agaagtatgc aaagcatgca tctcaattag
     6781 tcagcaacca ggtgtggaaa gtccccaggc tccccagcag gcagaagtat gcaaagcatg
     6841 catctcaatt agtcagcaac catagtcccg cccctaactc cgcccatccc gcccctaact
     6901 ccgcccagtt ccgcccattc tccgccccat ggctgactaa ttttttttat ttatgcagag
     6961 gccgaggccg cctctgcctc tgagctattc cagaagtagt gaggaggctt ttttggaggc
     7021 ctaggctttt gcaaaaagct cccgggagct tgtatatcca ttttcggatc tgatcaagag
     7081 acaggatgag gatcgtttcg catgattgaa caagatggat tgcacgcagg ttctccggcc
     7141 gcttgggtgg agaggctatt cggctatgac tgggcacaac agacaatcgg ctgctctgat
     7201 gccgccgtgt tccggctgtc agcgcagggg cgcccggttc tttttgtcaa gaccgacctg
     7261 tccggtgccc tgaatgaact gcaggacgag gcagcgcggc tatcgtggct ggccacgacg
     7321 ggcgttcctt gcgcagctgt gctcgacgtt gtcactgaag cgggaaggga ctggctgcta
     7381 ttgggcgaag tgccggggca ggatctcctg tcatctcacc ttgctcctgc cgagaaagta
     7441 tccatcatgg ctgatgcaat gcggcggctg catacgcttg atccggctac ctgcccattc
     7501 gaccaccaag cgaaacatcg catcgagcga gcacgtactc ggatggaagc cggtcttgtc
     7561 gatcaggatg atctggacga agagcatcag gggctcgcgc cagccgaact gttcgccagg
     7621 ctcaaggcgc gcatgcccga cggcgaggat ctcgtcgtga cccatggcga tgcctgcttg
     7681 ccgaatatca tggtggaaaa tggccgcttt tctggattca tcgactgtgg ccggctgggt
     7741 gtggcggacc gctatcagga catagcgttg gctacccgtg atattgctga agagcttggc
     7801 ggcgaatggg ctgaccgctt cctcgtgctt tacggtatcg ccgctcccga ttcgcagcgc
     7861 atcgccttct atcgccttct tgacgagttc ttctgagcgg gactctgggg ttcgcgaaat
     7921 gaccgaccaa gcgacgccca acctgccatc acgagatttc gattccaccg ccgccttcta
     7981 tgaaaggttg ggcttcggaa tcgttttccg ggacgccggc tggatgatcc tccagcgcgg
     8041 ggatctcatg ctggagttct tcgcccaccc caacttgttt attgcagctt ataatggtta
     8101 caaataaagc aatagcatca caaatttcac aaataaagca tttttttcac tgcattctag
     8161 ttgtggtttg tccaaactca tcaatgtatc ttatcatgtc tgtataccgt cgacctctag
     8221 ctagagcttg gcgtaatcat ggtcatagct gtttcctgtg tgaaattgtt atccgctcac
     8281 aattccacac aacatacgag ccggaagcat aaagtgtaaa gcctggggtg cctaatgagt
     8341 gagctaactc acattaattg cgttgcgctc actgcccgct ttccagtcgg gaaacctgtc
     8401 gtgccagctg cattaatgaa tcggccaacg cgcggggaga ggcggtttgc gtattgggcg
     8461 ctcttccgct tcctcgctca ctgactcgct gcgctcggtc gttcggctgc ggcgagcggt
     8521 atcagctcac tcaaaggcgg taatacggtt atccacagaa tcaggggata acgcaggaaa
     8581 gaacatgtga gcaaaaggcc agcaaaaggc caggaaccgt aaaaaggccg cgttgctggc
     8641 gtttttccat aggctccgcc cccctgacga gcatcacaaa aatcgacgct caagtcagag
     8701 gtggcgaaac ccgacaggac tataaagata ccaggcgttt ccccctggaa gctccctcgt
     8761 gcgctctcct gttccgaccc tgccgcttac cggatacctg tccgcctttc tcccttcggg
     8821 aagcgtggcg ctttctcata gctcacgctg taggtatctc agttcggtgt aggtcgttcg
     8881 ctccaagctg ggctgtgtgc acgaaccccc cgttcagccc gaccgctgcg ccttatccgg
     8941 taactatcgt cttgagtcca acccggtaag acacgactta tcgccactgg cagcagccac
     9001 tggtaacagg attagcagag cgaggtatgt aggcggtgct acagagttct tgaagtggtg
     9061 gcctaactac ggctacacta gaagaacagt atttggtatc tgcgctctgc tgaagccagt
     9121 taccttcgga aaaagagttg gtagctcttg atccggcaaa caaaccaccg ctggtagcgg
     9181 tggttttttt gttt
//
