LOCUS       sequence-410942-       18997 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  ePPE for prime editing in rice.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 18997)
  AUTHORS   Zhong Z, Fan T, He Y, Liu S, Zheng X, Xu Y, Ren J, Yuan H, Xu Z, 
            Zhang Y
  TITLE     An improved plant prime editor for efficient generation of 
            multiple-nucleotide variations and structural variations in rice.
  JOURNAL   Plant Commun. 2024 May 14:100976. doi: 10.1016/j.xplc.2024.100976.
  PUBMED    38751122
REFERENCE   2  (bases 1 to 18997)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Plant 
            Commun. 2024 May 14:100976. doi: 10.1016/j.xplc.2024.100976."
COMMENT     Sequence Label: pGEL686
FEATURES             Location/Qualifiers
     source          1..18997
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        8..2006
                     /label=Ubi promoter
                     /note="maize polyubiquitin gene promoter"
     CDS             join(2019..4535,4536..4538,4539..6122)
                     /codon_start=1
                     /product="nickase mutant of the Cas9 endonuclease from the 
                     Streptococcus pyogenes Type II CRISPR/Cas system"
                     /label=Cas9(H840A)
                     /note="generates RNA-guided single strand nicks in DNA due 
                     to the H840A mutation in the HNH catalytic domain"
                     /translation="MDKKYSIGLDIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKK
                     NLIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEES
                     FLVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIK
                     FRGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRL
                     ENLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQ
                     IGDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVR
                     QQLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLL
                     RKQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARG
                     NSRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEY
                     FTVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFD
                     SVEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEER
                     LKTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFM
                     QLIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGR
                     HKPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLY
                     LYYLQNGRDMYVDQELDINRLSDYDVDAIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVP
                     SEEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKH
                     VAQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYL
                     NAVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKT
                     EITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSK
                     ESILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGI
                     TIMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNE
                     LALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILAD
                     ANLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVL
                     DATLIHQSITGLYETRIDLSQLGGD"
     RBS             6035..6043
                     /label=Shine-Dalgarno sequence
                     /note="full consensus sequence for ribosome-binding sites 
                     upstream of start codons in E. coli; complementary to a 
                     region in the 3' end of the 16S rRNA (Chen et al., 1994)"
     CDS             6345..6392
                     /codon_start=1
                     /product="bipartite nuclear localization signal from 
                     nucleoplasmin"
                     /label=nucleoplasmin NLS
                     /translation="KRPAATKKAGQAKKKK"
     CDS             8046..8066
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     terminator      8075..8323
                     /label=HSP terminator
                     /note="efficient transcription terminator from the 
                     Arabidopsis thaliana heat shock protein 18.2 gene (Nagaya 
                     et al., 2010)"
     CDS             complement(8585..8890)
                     /codon_start=1
                     /gene="ccdB"
                     /product="CcdB, a bacterial toxin that poisons DNA gyrase"
                     /label=ccdB
                     /note="Plasmids containing the ccdB gene cannot be 
                     propagated in standard E. coli strains."
                     /translation="MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDK
                     VSRELYPVVHIGDESWRMMTTDMASVPVSVIGEEVADLSHRENDIKNAINLMFWGI"
     primer_bind     complement(8687..8706)
                     /label=ccdB-fwd
                     /note="ccdB gene, forward primer"
     protein_bind    complement(9221..9245)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     misc_feature    9275..9299
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     CDS             10600..11229
                     /codon_start=1
                     /product="stability protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 StaA
                     /translation="MKVIAVLNQKGGSGKTTIATHLARALQLAGADVLLVDSDPQGSAR
                     DWAAVREDQPLTVVGIDRPTIDRDVKAIGRRDFVVIDGAPQAADLAVSAIKAADFVLIP
                     VQPSPYDIWATADLVELVKQRIEVTDGRLQAAFVVSRAIKGTRIGGEVAEALAGYELPI
                     LESRITQRVSYPGTAAAGTTVLESEPEGDAAREVQALAAEIKSKLI"
     CDS             11658..12731
                     /codon_start=1
                     /product="replication protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 RepA
                     /translation="MSGRKPSGPVQIGAALGDDLVEKLKAAQAAQRQRIEAEARPGESW
                     QAAADRIRKESRQPPAAGAPSIRKPPKGDEQPDFFVPMLYDVGTRDSRSIMDVAVFRLS
                     KRDRRAGEVIRYELPDGHVEVSAGPAGMASVWDYDLVLMAVSHLTESMNRYREGKGDKP
                     GRVFRPHVADVLKFCRRADGGKQKDDLVETCIRLNTTHVAMQRTKKAKNGRLVTVSEGE
                     ALISRYKIVKSETGRPEYIEIELADWMYREITEGKNPDVLTVHPDYFLIDPGIGRFLYR
                     LARRAAGKAEARWLFKTIYERSGSAGEFKKFCFTVRKLIGSNDLPEYDLKEEAGQAGPI
                     LVMRYRNLIEGEASAGS"
     rep_origin      12797..12991
                     /label=pVS1 oriV
                     /note="origin of replication for the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
     primer_bind     complement(13227..13249)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     misc_feature    13325..13465
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     13380..13399
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     complement(13480..13497)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(13651..14239)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(13731..13750)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(15664..16458)
                     /codon_start=1
                     /gene="aphA-3"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin"
                     /translation="MAKMRISPELKKLIEKYRCVKDTEGMSPAKVYKLVGENENLYLKM
                     TDSRYKGTTYDVEREKDMMLWLEGKLPVPKVLHFERHDGWSNLLMSEADGVLCSEEYED
                     EQSPEKIIELYAECIRLFHSIDISDCPYTNSLDSRLAELDYLLNNDLADVDCENWEEDT
                     PFKDPRELYDFLKTEKPEEELVFSHGDLGDSNIFVKDGKVSGFIDLGRSGRADKWYDIA
                     FCVRSIREDIGEEQYVELFFDLLGIKPDWEKIKYYILLDELF"
     misc_feature    16883..16907
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     polyA_signal    16985..17159
                     /label=CaMV poly(A) signal
                     /note="cauliflower mosaic virus polyadenylation signal"
     promoter        complement(18249..18925)
                     /label=CaMV 35S promoter (enhanced)
                     /note="cauliflower mosaic virus 35S promoter with a 
                     duplicated enhancer region"
     primer_bind     complement(18292..18312)
                     /label=35S promoter
                     /note="CaMV 35S promoter, forward primer"
ORIGIN
        1 ggcgcgcctg cagtgcagcg tgacccggtc gtgcccctct cttgagataa ggtgagcatt
       61 gcatgtctaa gttataaaaa attaccacat attttttttg tcacacttgt ttgaagtgca
      121 gtttatctat ctttatacat atatttaaac tttactctac gaataatata atctatagta
      181 ctacaataat atcagtgttt tagagaatca tataaatgaa cagttagaca tggtctaaag
      241 gacaattgag tattttgaca acaggactct acagttttat ctttttagtg tgcatgtgtt
      301 ctcctttttt tttgcaaata gcttcaccta tataatactt catccatttt attagtacat
      361 ccatttaggg tttagggtta atggttttta tagactaatt tttttagtac atctatttta
      421 ttctatttta gcctctaaat taagaaaact aaaactctat tttagttttt ttatttaata
      481 atttagatat aaaatagaat aaaataaagt gactaaaaat taaacaaata ccctttaaga
      541 aattaaaaaa actaaggaaa catttttctt gtttcgagta gataatgcca gcctgttaaa
      601 cgccgtcgac gagtctaacg gacaccaacc agcgaaccag cagcgtcgcg tcgggccaag
      661 cgaagcagac ggcacggcat ctctgtcgct gcctctggac ccctctcgag agttccgctc
      721 caccgttgga cttgctccgc tgtcggcatc cagaaattgc gtggcggagc ggcagacgtg
      781 agccggcacg gcaggcggcc tcctcctcct ctcacggcac cggcagctac gggggattcc
      841 tttcccaccg ctccttcgct ttcccttcct cgcccgccgt aataaataga caccccctcc
      901 acaccctctt tccccaacct cgtgttgttc ggagcgcaca cacacacaac cagaactccc
      961 ccaaatccac ccgtcggcac ctccgcttca aggtacgccg ctcgtcctcc cccccccccc
     1021 ctctctacct tctcaagatc ggcgttccgg tccatggtta gggcccggta gttctacttc
     1081 tgttcatgtt tgtgttagat ccgtgtttgt gttagatccg tgctactagc gttcgtacac
     1141 ggatgcgacc tgtacgtcag acacgttctg attgctaact tgccagtgtt tctctttggg
     1201 gaatcctggg atggctctag ccgttccgca gacgggatcg atttcatgat tttttttgtt
     1261 tcgttgcata gggtttggtt tgcccttttc ctttatttca atatatgccg tgcacttgtt
     1321 tgtcgggtca tcttttcatg cttttttttg tcttggttgt gatgatgtgg tctggttggg
     1381 cggtcgttca agatcggagt agaattaatt ctgtttcaaa ctacctggtg gatttattaa
     1441 ttttggatct gtatgtgtgt gccatacata ttcatagtta cgaattgaag atgatggatg
     1501 gaaatatcga tctaggatag gtatacatgt tgatgcgggt tttactgatg catatacaga
     1561 gatgcttttt gttcgcttgg ttgtgatgat gtggtgtggt tgggcggtcg ttcattcgtt
     1621 caagatcgga gtagaatact gtttcaaact acctggtgta tttattaatt ttggaactgt
     1681 atgtgtgtgt catacatctt catagttacg agtttaagat ggatggaaat atcgatctag
     1741 gataggtata catgttgatg tgggttttac tgatgcatat acatgatggc atatgcagca
     1801 tctattcata tgctctaacc ttgagtacct atctattata ataaacaagt atgttttata
     1861 attattttga tcttgatata cttggatgat ggcatatgca gcagctatat gtggattttt
     1921 ttagccctgc cttcatacgc tatttatttg cttggtactg tttcttttgt cgatgctcac
     1981 cctgttgttt ggtgttactt ctgcagcctg caggccatat ggacaagaag tactcgatcg
     2041 gcctcgatat tgggactaac tctgttggct gggccgtgat caccgacgag tacaaggtgc
     2101 cctcaaagaa gttcaaggtc ctgggcaaca ccgatcggca ttccatcaag aagaatctca
     2161 ttggcgctct cctgttcgac agcggcgaga cggctgaggc tacgcggctc aagcgcaccg
     2221 cccgcaggcg gtacacgcgc aggaagaatc gcatctgcta cctgcaggag attttctcca
     2281 acgagatggc gaaggttgac gattctttct tccacaggct ggaggagtca ttcctcgtgg
     2341 aggaggataa gaagcacgag cggcatccaa tcttcggcaa cattgtcgac gaggttgcct
     2401 accacgagaa gtaccctacg atctaccatc tgcggaagaa gctcgtggac tccacagata
     2461 aggcggacct ccgcctgatc tacctcgctc tggcccacat gattaagttc aggggccatt
     2521 tcctgatcga gggggatctc aacccggaca atagcgatgt tgacaagctg ttcatccagc
     2581 tcgtgcagac gtacaaccag ctcttcgagg agaaccccat taatgcgtca ggcgtcgacg
     2641 cgaaggctat cctgtccgct aggctctcga agtctcggcg cctcgagaac ctgatcgccc
     2701 agctgccggg cgagaagaag aacggcctgt tcgggaatct cattgcgctc agcctggggc
     2761 tcacgcccaa cttcaagtcg aatttcgatc tcgctgagga cgccaagctg cagctctcca
     2821 aggacacata cgacgatgac ctggataacc tcctggccca gatcggcgat cagtacgcgg
     2881 acctgttcct cgctgccaag aatctgtcgg acgccatcct cctgtctgat attctcaggg
     2941 tgaacaccga gattacgaag gctccgctct cagcctccat gatcaagcgc tacgacgagc
     3001 accatcagga tctgaccctc ctgaaggcgc tggtcaggca gcagctcccc gagaagtaca
     3061 aggagatctt cttcgatcag tcgaagaacg gctacgctgg gtacattgac ggcggggcct
     3121 ctcaggagga gttctacaag ttcatcaagc cgattctgga gaagatggac ggcacggagg
     3181 agctgctggt gaagctcaat cgcgaggacc tcctgaggaa gcagcggaca ttcgataacg
     3241 gcagcatccc acaccagatt catctcgggg agctgcacgc tatcctgagg aggcaggagg
     3301 acttctaccc tttcctcaag gataaccgcg agaagatcga gaagattctg actttcagga
     3361 tcccgtacta cgtcggccca ctcgctaggg gcaactcccg cttcgcttgg atgacccgca
     3421 agtcagagga gacgatcacg ccgtggaact tcgaggaggt ggtcgacaag ggcgctagcg
     3481 ctcagtcgtt catcgagagg atgacgaatt tcgacaagaa cctgccaaat gagaaggtgc
     3541 tccctaagca ctcgctcctg tacgagtact tcacagtcta caacgagctg actaaggtga
     3601 agtatgtgac cgagggcatg aggaagccgg ctttcctgtc tggggagcag aagaaggcca
     3661 tcgtggacct cctgttcaag accaaccgga aggtcacggt taagcagctc aaggaggact
     3721 acttcaagaa gattgagtgc ttcgattcgg tcgagatctc tggcgttgag gaccgcttca
     3781 acgcctccct ggggacctac cacgatctcc tgaagatcat taaggataag gacttcctgg
     3841 acaacgagga gaatgaggat atcctcgagg acattgtgct gacactcact ctgttcgagg
     3901 accgggagat gatcgaggag cgcctgaaga cttacgccca tctcttcgat gacaaggtca
     3961 tgaagcagct caagaggagg aggtacaccg gctgggggag gctgagcagg aagctcatca
     4021 acggcattcg ggacaagcag tccgggaaga cgatcctcga cttcctgaag agcgatggct
     4081 tcgcgaaccg caatttcatg cagctgattc acgatgacag cctcacattc aaggaggata
     4141 tccagaaggc tcaggtgagc ggccaggggg actcgctgca cgagcatatc gcgaacctcg
     4201 ctggctcgcc agctatcaag aaggggattc tgcagaccgt gaaggttgtg gacgagctgg
     4261 tgaaggtcat gggcaggcac aagcctgaga acatcgtcat tgagatggcc cgggagaatc
     4321 agaccacgca gaagggccag aagaactcac gcgagaggat gaagaggatc gaggagggca
     4381 ttaaggagct ggggtcccag atcctcaagg agcacccggt ggagaacacg cagctgcaga
     4441 atgagaagct ctacctgtac tacctccaga atggccgcga tatgtatgtg gaccaggagc
     4501 tggatattaa caggctcagc gattacgacg tcgatgctat cgttccacag tcattcctga
     4561 aggatgactc cattgacaac aaggtcctca ccaggtcgga caagaaccgg ggcaagtctg
     4621 ataatgttcc ttcagaggag gtcgttaaga agatgaagaa ctactggcgc cagctcctga
     4681 atgccaagct gatcacgcag cggaagttcg ataacctcac aaaggctgag aggggcgggc
     4741 tctctgagct ggacaaggcg ggcttcatca agaggcagct ggtcgagaca cggcagatca
     4801 ctaagcacgt tgcgcagatt ctcgactcac ggatgaacac taagtacgat gagaatgaca
     4861 agctgatccg cgaggtgaag gtcatcaccc tgaagtcaaa gctcgtctcc gacttcagga
     4921 aggatttcca gttctacaag gttcgggaga tcaacaatta ccaccatgcc catgacgcgt
     4981 acctgaacgc ggtggtcggc acagctctga tcaagaagta cccaaagctc gagagcgagt
     5041 tcgtgtacgg ggactacaag gtttacgatg tgaggaagat gatcgccaag tcggagcagg
     5101 agattggcaa ggctaccgcc aagtacttct tctactctaa cattatgaat ttcttcaaga
     5161 cagagatcac tctggccaat ggcgagatcc ggaagcgccc cctcatcgag acgaacggcg
     5221 agacggggga gatcgtgtgg gacaagggca gggatttcgc gaccgtcagg aaggttctct
     5281 ccatgccaca agtgaatatc gtcaagaaga cagaggtcca gactggcggg ttctctaagg
     5341 agtcaattct gcctaagcgg aacagcgaca agctcatcgc ccgcaagaag gactgggatc
     5401 cgaagaagta cggcgggttc gacagcccca ctgtggccta ctcggtcctg gttgtggcga
     5461 aggttgagaa gggcaagtcc aagaagctca agagcgtgaa ggagctgctg gggatcacga
     5521 ttatggagcg ctccagcttc gagaagaacc cgatcgattt cctggaggcg aagggctaca
     5581 aggaggtgaa gaaggacctg atcattaagc tccccaagta ctcactcttc gagctggaga
     5641 acggcaggaa gcggatgctg gcttccgctg gcgagctgca gaaggggaac gagctggctc
     5701 tgccgtccaa gtatgtgaac ttcctctacc tggcctccca ctacgagaag ctcaagggca
     5761 gccccgagga caacgagcag aagcagctgt tcgtcgagca gcacaagcat tacctcgacg
     5821 agatcattga gcagatttcc gagttctcca agcgcgtgat cctggccgac gcgaatctgg
     5881 ataaggtcct ctccgcgtac aacaagcacc gcgacaagcc aatcagggag caggctgaga
     5941 atatcattca tctcttcacc ctgacgaacc tcggcgcccc tgctgctttc aagtacttcg
     6001 acacaactat cgatcgcaag aggtacacaa gcactaagga ggtcctggac gcgaccctca
     6061 tccaccagtc gattaccggc ctctacgaga cgcgcatcga cctgtctcag ctcgggggcg
     6121 acgaattctc cgggagcgag acgccaggca cctccgagtc ggccacccca gaatctgcca
     6181 cagtggtgtc cggccaaaag caggaccgcc agggcggaga acgcagaagg tcccagctcg
     6241 atagggatca gtgtgcctac tgcaaggaga agggccactg ggccaaagac tgcccgaaaa
     6301 agccgcgcgg cccacgcggc ccaaggccac aaacatccct ccttaagcgg ccagcggcga
     6361 cgaagaaggc ggggcaggcg aagaagaaga agtcaggggg ttctagcggc ggttcatctg
     6421 gctccgaaac accgggaact tccgagagtg ctactccaga atctagcggt gggtcatctg
     6481 gagggtcttc cacacttaat attgaggacg aataccgcct gcatgaaact agtaaagaac
     6541 ccgacgtgag cctcggtagc acgtggctct ctgatttccc gcaagcatgg gctgaaacag
     6601 gtgggatggg cctcgctgtt cgccaggctc cattgatcat cccactcaag gccacttcta
     6661 caccggtgtc cataaagcaa tacccaatga gccaggaagc tcggcttggt atcaagcctc
     6721 acatccaaag attgcttgat cagggaattc ttgtcccttg tcaaagtcca tggaacaccc
     6781 ctcttcttcc cgtcaaaaaa cctgggacta acgattatag gccagtgcaa gacctcaggg
     6841 aggttaacaa aagggttgaa gacattcatc caacagtgcc gaatccttat aatctgctct
     6901 caggtttgcc tccaagtcac cagtggtata cagtgctgga tcttaaagac gcattttttt
     6961 gtctgcgcct tcatcccact tcgcaacctc tttttgcttt cgaatggaga gatccagaga
     7021 tgggtatctc cggacagctc acatggaccc ggttgcccca aggcttcaaa aactccccaa
     7081 ccttgtttaa tgaagcgctg cacagagatt tggcggactt caggatccag caccccgact
     7141 tgattctcct gcagtatgtg gatgaccttt tgcttgcagc cacatctgag cttgactgcc
     7201 aacaggggac gcgcgccctt cttcagactt tgggaaacct tgggtataga gcctctgcca
     7261 agaaggcaca gatctgccag aagcaagtta agtatctcgg ttatttgctc aaggagggtc
     7321 agaggtggct gacggaggcg aggaaagaaa cggtgatggg ccaaccaacc ccaaaaaccc
     7381 ctaggcaact tagagagttt cttggtaaag cggggttttg ccggttgttc attccaggct
     7441 ttgccgagat ggccgcaccg ttgtatccat tgacgaaacc gggcaccctt tttaattggg
     7501 ggcccgacca gcagaaggcg tatcaggaga tcaagcaagc tctcctgacc gcaccagcac
     7561 tcggacttcc agatcttacg aaaccgtttg agctcttcgt ggatgagaaa caggggtatg
     7621 ctaagggagt cctcacacaa aagttgggac cgtggaggcg gccggttgcc tatctttcga
     7681 aaaaacttga tcccgtggcg gctggttggc ctccctgtct gaggatggtg gccgcgatcg
     7741 cagtccttac aaaagatgca gggaagttga ctatgggaca accattggtc atacttgccc
     7801 cgcacgcggt tgaggccctc gttaagcagc ctcccgatag gtggctcagt aacgcccgca
     7861 tgactcatta ccaggcactg cttctcgaca cagacagggt gcagtttggt cccgtcgtcg
     7921 ctttgaaccc cgccaccctg ctgcccctcc cggaggaagg attgcagcac aactgtctgg
     7981 atatcttggc cgaagcgcat gggactcgcc cggacttgac tgaccagccc cttagcggcg
     8041 gcagcccgaa gaaaaagaga aaggtgtgag cttatatgaa gatgaagatg aaatatttgg
     8101 tgtgtcaaat aaaaagcttg tgtgcttaag tttgtgtttt tttcttggct tgttgtgtta
     8161 tgaatttgtg gctttttcta atattaaatg aatgtaagat cacattataa tgaataaaca
     8221 aatgtttcta taatccattg tgaatgtttt gttggatctc ttctgcagca tataactact
     8281 gtatgtgcta tggtatggac tatggaatat gattaaagat aagaggatcc gcggatcatg
     8341 aaccaacggc ctggctgtat ttggtggttg tgtagggaga tggggagaag aaaagcccga
     8401 ttctcttcgc tgtgatgggc tggatgcatg cgggggagcg ggaggcccaa gtacgtgcac
     8461 ggtgagcggc ccacagggcg agtgtgagcg cgagaggcgg gaggaacagt ttagtaccac
     8521 attgcccagc taactcgaac gcgaccaact tataaacccg cgcgctgtcg cttgtgtgga
     8581 gaccttatat tccccagaac atcaggttaa tggcgttttt gatgtcattt tcgcggtggc
     8641 tgagatcagc cacttcttcc ccgataacgg aaaccggcac actggccata tcggtggtca
     8701 tcatgcgcca gctttcatcc ccgatatgca ccaccgggta aagttcacgg gagactttat
     8761 ctgacagcag acgtgcactg gccaggggga tcaccatccg tcgcccgggc gtgtcaataa
     8821 tatcactctg tacatccaca aacagacgat aacggctctc tcttttatag gtgtaaacct
     8881 taaactgcat ttcaccagcc cctgttctcg tcagcaaaag agccgttcat ttcaataaac
     8941 cgggcgacct cagccatccc ttcctgattt tccgctttcc agcgttcggc acgcagacga
     9001 cgggcttcat tctgcatggt tgtgcttacc agaccggaga tattgacatc atatatgcct
     9061 tgagcaactg atagctgtcg ctgtcaactg tcactgtaat acgctgcttc atagcatacc
     9121 tctttttgac atacttcggg tatacatatc agtatatatt cttataccgc aaaaatcagc
     9181 gcgcaaatac gcatactgtt atctggcttg gtctctctag acccagcttt cttgtacaaa
     9241 gttggcatta ctgcgagctc aaactatcag tgtttgacag gatatattgg cgggtaaacc
     9301 taagagaaaa gagcgtttat tagaataatc ggatatttaa aagggcgtga aaaggtttat
     9361 ccgttcgtcc atttgtatgt gcatgccaac cacagggttc ccctcgggat caaagtactt
     9421 tgatccaacc cctccgctgc tatagtgcag tcggcttctg acgttcagtg cagccgtctt
     9481 ctgaaaacga catgtcgcac aagtcctaag ttacgcgaca ggctgccgcc ctgccctttt
     9541 cctggcgttt tcttgtcgcg tgttttagtc gcataaagta gaatacttgc gactagaacc
     9601 ggagacatta cgccatgaac aagagcgccg ccgctggcct gctgggctat gcccgcgtca
     9661 gcaccgacga ccaggacttg accaaccaac gggccgaact gcacgcggcc ggctgcacca
     9721 agctgttttc cgagaagatc accggcacca ggcgcgaccg cccggagctg gccaggatgc
     9781 ttgaccacct acgccctggc gacgttgtga cagtgaccag gctagaccgc ctggcccgca
     9841 gcacccgcga cctactggac attgccgagc gcatccagga ggccggcgcg ggcctgcgta
     9901 gcctggcaga gccgtgggcc gacaccacca cgccggccgg ccgcatggtg ttgaccgtgt
     9961 tcgccggcat tgccgagttc gagcgttccc taatcatcga ccgcacccgg agcgggcgcg
    10021 aggccgccaa ggcccgaggc gtgaagtttg gcccccgccc taccctcacc ccggcacaga
    10081 tcgcgcacgc ccgcgagctg atcgaccagg aaggccgcac cgtgaaagag gcggctgcac
    10141 tgcttggcgt gcatcgctcg accctgtacc gcgcacttga gcgcagcgag gaagtgacgc
    10201 ccaccgaggc caggcggcgc ggtgccttcc gtgaggacgc attgaccgag gccgacgccc
    10261 tggcggccgc cgagaatgaa cgccaagagg aacaagcatg aaaccgcacc aggacggcca
    10321 ggacgaaccg tttttcatta ccgaagagat cgaggcggag atgatcgcgg ccgggtacgt
    10381 gttcgagccg cccgcgcacg gctcaaccgt gcggctgcat gaaatcctgg ccggtttgtc
    10441 tgatgccaag ctggcggcct ggccggccag cttggccgct gaagaaaccg agcgccgccg
    10501 tctaaaaagg tgatgtgtat ttgagtaaaa cagcttgcgt catgcggtcg ctgcgtatat
    10561 gatgcgatga gtaaataaac aaatacgcaa ggggaacgca tgaaggttat cgctgtactt
    10621 aaccagaaag gcgggtcagg caagacgacc atcgcaaccc atctagcccg cgccctgcaa
    10681 ctcgccgggg ccgatgttct gttagtcgat tccgatcccc agggcagtgc ccgcgattgg
    10741 gcggccgtgc gggaagatca accgctaacc gttgtcggca tcgaccgccc gacgattgac
    10801 cgcgacgtga aggccatcgg ccggcgcgac ttcgtagtga tcgacggagc gccccaggcg
    10861 gcggacttgg ctgtgtccgc gatcaaggca gccgacttcg tgctgattcc ggtgcagcca
    10921 agcccttacg acatatgggc caccgccgac ctggtggagc tggttaagca gcgcattgag
    10981 gtcacggatg gaaggctaca agcggccttt gtcgtgtcgc gggcgatcaa aggcacgcgc
    11041 atcggcggtg aggttgccga ggcgctggcc gggtacgagc tgcccattct tgagtcccgt
    11101 atcacgcagc gcgtgagcta cccaggcact gccgccgccg gcacaaccgt tcttgaatca
    11161 gaacccgagg gcgacgctgc ccgcgaggtc caggcgctgg ccgctgaaat taaatcaaaa
    11221 ctcatttgag ttaatgaggt aaagagaaaa tgagcaaaag cacaaacacg ctaagtgccg
    11281 gccgtccgag cgcacgcagc agcaaggctg caacgttggc cagcctggca gacacgccag
    11341 ccatgaagcg ggtcaacttt cagttgccgg cggaggatca caccaagctg aagatgtacg
    11401 cggtacgcca aggcaagacc attaccgagc tgctatctga atacatcgcg cagctaccag
    11461 agtaaatgag caaatgaata aatgagtaga tgaattttag cggctaaagg aggcggcatg
    11521 gaaaatcaag aacaaccagg caccgacgcc gtggaatgcc ccatgtgtgg aggaacgggc
    11581 ggttggccag gcgtaagcgg ctgggttgcc tgccggccct gcaatggcac tggaaccccc
    11641 aagcccgagg aatcggcgtg agcggtcgca aaccatccgg cccggtacaa atcggcgcgg
    11701 cgctgggtga tgacctggtg gagaagttga aggccgcgca ggccgcccag cggcaacgca
    11761 tcgaggcaga agcacgcccc ggtgaatcgt ggcaagcggc cgctgatcga atccgcaaag
    11821 aatcccggca accgccggca gccggtgcgc cgtcgattag gaagccgccc aagggcgacg
    11881 agcaaccaga ttttttcgtt ccgatgctct atgacgtggg cacccgcgat agtcgcagca
    11941 tcatggacgt ggccgttttc cgtctgtcga agcgtgaccg acgagctggc gaggtgatcc
    12001 gctacgagct tccagacggg cacgtagagg tttccgcagg gccggccggc atggccagtg
    12061 tgtgggatta cgacctggta ctgatggcgg tttcccatct aaccgaatcc atgaaccgat
    12121 accgggaagg gaagggagac aagcccggcc gcgtgttccg tccacacgtt gcggacgtac
    12181 tcaagttctg ccggcgagcc gatggcggaa agcagaaaga cgacctggta gaaacctgca
    12241 ttcggttaaa caccacgcac gttgccatgc agcgtacgaa gaaggccaag aacggccgcc
    12301 tggtgacggt atccgagggt gaagccttga ttagccgcta caagatcgta aagagcgaaa
    12361 ccgggcggcc ggagtacatc gagatcgagc tagctgattg gatgtaccgc gagatcacag
    12421 aaggcaagaa cccggacgtg ctgacggttc accccgatta ctttttgatc gatcccggca
    12481 tcggccgttt tctctaccgc ctggcacgcc gcgccgcagg caaggcagaa gccagatggt
    12541 tgttcaagac gatctacgaa cgcagtggca gcgccggaga gttcaagaag ttctgtttca
    12601 ccgtgcgcaa gctgatcggg tcaaatgacc tgccggagta cgatttgaag gaggaggcgg
    12661 ggcaggctgg cccgatccta gtcatgcgct accgcaacct gatcgagggc gaagcatccg
    12721 ccggttccta atgtacggag cagatgctag ggcaaattgc cctagcaggg gaaaaaggtc
    12781 gaaaaggcct ctttcctgtg gatagcacgt acattgggaa cccaaagccg tacattggga
    12841 accggaaccc gtacattggg aacccaaagc cgtacattgg gaaccggtca cacatgtaag
    12901 tgactgatat aaaagagaaa aaaggcgatt tttccgccta aaactcttta aaacttatta
    12961 aaactcttaa aacccgcctg gcctgtgcat aactgtctgg ccagcgcaca gccgaagagc
    13021 tgcaaaaagc gcctaccctt cggtcgctgc gctccctacg ccccgccgct tcgcgtcggc
    13081 ctatcgcggc cgctggccgc tcaaaaatgg ctggcctacg gccaggcaat ctaccagggc
    13141 gcggacaagc cgcgccgtcg ccactcgacc gccggcgccc acatcaaggc accctgcctc
    13201 gcgcgtttcg gtgatgacgg tgaaaacctc tgacacatgc agctcccgga aacggtcaca
    13261 gcttgtctgt aagcggatgc cgggagcaga caagcgcgtc agcgggtgtt ggcgggtgtc
    13321 ggggcgcagc catgacccag tcacgtagcg atagcggagt gtatactggc ttaactatgc
    13381 ggcatcagag cagattgtac tgagagtgca ccatatgcgg tgtgaaatac cgcacagatg
    13441 cgtaaggaga aaataccgca tcaggcgctc ttccgcttcc tcgctcactg actcgctgcg
    13501 ctcggtcgtt cggctgcggc gagcggtatc agctcactca aaggcggtaa tacggttatc
    13561 cacagaatca ggggataacg caggaaagaa catgtgagca aaaggccagc aaaaggccag
    13621 gaaccgtaaa aaggccgcgt tgctggcgtt tttccatagg ctccgccccc ctgacgagca
    13681 tcacaaaaat cgacgctcaa gtcagaggtg gcgaaacccg acaggactat aaagatacca
    13741 ggcgtttccc cctggaagct ccctcgtgcg ctctcctgtt ccgaccctgc cgcttaccgg
    13801 atacctgtcc gcctttctcc cttcgggaag cgtggcgctt tctcatagct cacgctgtag
    13861 gtatctcagt tcggtgtagg tcgttcgctc caagctgggc tgtgtgcacg aaccccccgt
    13921 tcagcccgac cgctgcgcct tatccggtaa ctatcgtctt gagtccaacc cggtaagaca
    13981 cgacttatcg ccactggcag cagccactgg taacaggatt agcagagcga ggtatgtagg
    14041 cggtgctaca gagttcttga agtggtggcc taactacggc tacactagaa ggacagtatt
    14101 tggtatctgc gctctgctga agccagttac cttcggaaaa agagttggta gctcttgatc
    14161 cggcaaacaa accaccgctg gtagcggtgg tttttttgtt tgcaagcagc agattacgcg
    14221 cagaaaaaaa ggatctcaag aagatccttt gatcttttct acggggtctg acgctcagtg
    14281 gaacgaaaac tcacgttaag ggctgatgaa tcccctaatg atttttatca aaatcattaa
    14341 gttaaggtag atacacatct tgtcatatga tcaaatggtt tcgccaaaaa tcaataatca
    14401 gacaacaaaa tgtgcgaact cgatatttta cacgactctc tttaccaatt ctgccccgaa
    14461 ttacacttaa aacgactcaa cagcttaacg ttggcttgcc acgccttact tgactgtaaa
    14521 actctcactc ttaccgaact tggccgtaac ctgccaacca aagcgagaac aaaacataac
    14581 atcaaacgaa tcgaccgatt gttaggtaat cgtcacctcc acaaagagcg actcgctgta
    14641 taccgttggc atgctagctt tatctgttcg ggcaatacga tgcccattgt acttgttgac
    14701 tggtctgata tccgtgagca aaaacggctt atggtattgc gagcttcagt cgcactacac
    14761 ggtcgttctg ttactcttta tgagaaagcg ttcccgcttt cagagcaatg ttcaaagaaa
    14821 gctcatgacc aatttctagc cgaccttgcg agcattctac cgagtaacac cacaccgctc
    14881 attgtcagtg atgctggctt taaagtgcca tggtataaat ccgttgagaa gctgggttgg
    14941 tactggttaa gtcgagtaag aggaaaagta caatatgcag acctaggagc ggaaaactgg
    15001 aaacctatca gcaacttaca tgatatgtca tctagtcact caaagacttt aggctataag
    15061 aggctgacta aaagcaatcc aatctcatgc caaattctat tgtataaatc tcgctctaaa
    15121 ggccgaaaaa atcagcgctc gacacggact cattgtcacc acccgtcacc taaaatctac
    15181 tcagcgtcgg caaaggagcc atggattcta gcaactaact tacctgttga aattcgaaca
    15241 cccaaacaac ttgttaatat ctattcgaag cgaatgcaga ttgaagaaac cttccgagac
    15301 ttgaaaagtc ctgcctacgg actaggccta cgccatagcc gaacgagcag ctcagagcgt
    15361 tttgatatca tgctgctaat cgccctgatg cttcaactaa catgttggct tgcgggcgtt
    15421 catgctcaga aacaaggttg ggacaagcac ttccaggcta acacagtcag aaatcgaaac
    15481 gtactctcaa cagttcgctt aggcatggaa gttttgcggc attctggcta cacaataaca
    15541 agggaagact tactcgtggc tgcaacccta ctagctcaaa atttattcac acatggttac
    15601 gctttgggga aattatgagg ggatctctca gcgttaaggg attttggtca tgcattctag
    15661 gtactaaaac aattcatcca gtaaaatata atattttatt ttctcccaat caggcttgat
    15721 ccccagtaag tcaaaaaata gctcgacata ctgttcttcc ccgatatcct ccctgatcga
    15781 ccggacgcag aaggcaatgt cataccactt gtccgccctg ccgcttctcc caagatcaat
    15841 aaagccactt actttgccat ctttcacaaa gatgttgctg tctcccaggt cgccgtggga
    15901 aaagacaagt tcctcttcgg gcttttccgt ctttaaaaaa tcatacagct cgcgcggatc
    15961 tttaaatgga gtgtcttctt cccagttttc gcaatccaca tcggccagat cgttattcag
    16021 taagtaatcc aattcggcta agcggctgtc taagctattc gtatagggac aatccgatat
    16081 gtcgatggag tgaaagagcc tgatgcactc cgcatacagc tcgataatct tttcagggct
    16141 ttgttcatct tcatactctt ccgagcaaag gacgccatcg gcctcactca tgagcagatt
    16201 gctccagcca tcatgccgtt caaagtgcag gacctttgga acaggcagct ttccttccag
    16261 ccatagcatc atgtcctttt cccgttccac atcataggtg gtccctttat accggctgtc
    16321 cgtcattttt aaatataggt tttcattttc tcccaccagc ttatatacct tagcaggaga
    16381 cattccttcc gtatctttta cgcagcggta tttttcgatc agttttttca attccggtga
    16441 tattctcatt ttagccattt attatttcct tcctcttttc tacagtattt aaagataccc
    16501 caagaagcta attataacaa gacgaactcc aattcactgt tccttgcatt ctaaaacctt
    16561 aaataccaga aaacagcttt ttcaaagttg ttttcaaagt tggcgtataa catagtatcg
    16621 acggagccga ttttgaaacc gcggtgatca caggcagcaa cgctctgtca tcgttacaat
    16681 caacatgcta ccctccgcga gatcatccgt gtttcaaacc cggcagctta gttgccgttc
    16741 ttccgaatag catcggtaac atgagcaaag tctgccgcct tacaacggct ctcccgctga
    16801 cgccgtcccg gactgatggg ctgcctgtat cgagtggtga ttttgtgccg agctgccggt
    16861 cggggagctg ttggctggct ggtggcagga tatattgtgg tgtaaacaaa ttgacgctta
    16921 gacaacttaa taacacattg cggacgtttt taatgtactg aattaacgcc gaattaattc
    16981 gggggatctg gattttagta ctggattttg gttttaggaa ttagaaattt tattgataga
    17041 agtattttac aaatacaaat acatactaag ggtttcttat atgctcaaca catgagcgaa
    17101 accctatagg aaccctaatt cccttatctg ggaactactc acacattatt atggagaaaa
    17161 tagagcttgt cgatcgttaa ttaatcacct ccaagatccc ggacgagtgc tggggcgtcg
    17221 gtttccacta tcggcgagta cttctacaca gccatcggtc cagacggccg cgcttctgcg
    17281 ggcgatttgt gtacgcccga cagtcccggc tccggatcgg acgattgcgt cgcatcgacc
    17341 ctgcgcccaa gctgcatcat cgaaattgcc gtcaaccaag ctctgataga gttggtcaag
    17401 accaatgcgg agcatatacg cccggagccg cggcgatcct gcaagctccg gatgcctccg
    17461 ctcgaagtag cgcgtctgct gctccataca agccaaccac ggcctccaga agaagatgtt
    17521 ggcgacctcg tattgggaat ccccgaacat cgcctcgctc cagtcaatga ccgctgttat
    17581 gcggccattg tccgtcagga cattgttgga gccgaaatcc gcgtgcacga ggtgccggac
    17641 ttcggggcag tcctcggccc aaagcatcag ctcatcgaga gcctgcgcga cggacgcact
    17701 gacggtgtcg tccatcacag tttgccagtg atacacatgg ggatcagcaa tcgcgcatat
    17761 gaaatcacgc catgtagtgt attgaccgat tccttgcggt ccgaatgggc cgaacccgct
    17821 cgtctggcta agatcggccg cagcgatcgc atccatggcc tccgcgaccg gctgcagaac
    17881 agcgggcagt tcggtttcag gcaggtcttg caacgtgaca ccctgtgcac ggcgggagat
    17941 gcaataggtc aggctctcgc tgaattcccc aatgtcaagc acttccggaa tcgggagcgc
    18001 ggccgatgca aagtgccgat aaacataacg atctttgtag aaaccatcgg cgcagctatt
    18061 tacccgcagg acatatccac gccctcctac atcgaagctg aaagcacgag attcttcgcc
    18121 ctccgagagc tgcatcaggt cggacacgct gtcgaacttt tcgatcagaa acttctcgac
    18181 agacgtcgcg gtgagttcag gctttttcat cctaggagag atagatttgt agagagagac
    18241 tggtgatttc agcgtgtcct ctccaaatga aatgaacttc cttatataga ggaagggtct
    18301 tgcgaaggat agtgggattg tgcgtcatcc cttacgtcag tggagatatc acatcaatcc
    18361 acttgctttg aagacgtggt tggaacgtct tctttttcca cgatgctcct cgtgggtggg
    18421 ggtccatctt tgggaccact gtcggcagag gcatcttgaa cgatagcctt tcctttatcg
    18481 caatgatggc atttgtaggt gccaccttcc ttttctactg tccttttgat gaagtgacag
    18541 atagctgggc aatggaatcc gaggaggttt cccgatatta ccctttgttg aaaagtctca
    18601 atagcccttt ggtcttctga gactgtatct ttgatattct tggagtagac gagagtgtcg
    18661 tgctccacca tgttcacatc aatccacttg ctttgaagac gtggttggaa cgtcttcttt
    18721 ttccacgatg ctcctcgtgg gtgggggtcc atctttggga ccactgtcgg cagaggcatc
    18781 ttgaacgata gcctttcctt tatcgcaatg atggcatttg taggtgccac cttccttttc
    18841 tactgtcctt ttgatgaagt gacagatagc tgggcaatgg aatccgagga ggtttcccga
    18901 tattaccctt tgttgaaaag tctcaatagc cctttggtct tctgagactg tatctttgat
    18961 attcttggag tagacgagag tgtcgtgctc caccatg
//
