LOCUS       sequence-410943-       19294 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Cas9-PE2 for prime editing in rice.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 19294)
  AUTHORS   Zhong Z, Fan T, He Y, Liu S, Zheng X, Xu Y, Ren J, Yuan H, Xu Z, 
            Zhang Y
  TITLE     An improved plant prime editor for efficient generation of 
            multiple-nucleotide variations and structural variations in rice.
  JOURNAL   Plant Commun. 2024 May 14:100976. doi: 10.1016/j.xplc.2024.100976.
  PUBMED    38751122
REFERENCE   2  (bases 1 to 19294)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Plant 
            Commun. 2024 May 14:100976. doi: 10.1016/j.xplc.2024.100976."
COMMENT     Sequence Label: pGEL683
FEATURES             Location/Qualifiers
     source          1..19294
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        8..2006
                     /label=Ubi promoter
                     /note="maize polyubiquitin gene promoter"
     CDS             2019..6122
                     /codon_start=1
                     /product="Cas9 (Csn1) endonuclease from the Streptococcus 
                     pyogenes Type II CRISPR/Cas system"
                     /label=Cas9
                     /note="generates RNA-guided double strand breaks in DNA"
                     /translation="MDKKYSIGLDIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKK
                     NLIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEES
                     FLVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIK
                     FRGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRL
                     ENLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQ
                     IGDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVR
                     QQLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLL
                     RKQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARG
                     NSRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEY
                     FTVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFD
                     SVEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEER
                     LKTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFM
                     QLIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGR
                     HKPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLY
                     LYYLQNGRDMYVDQELDINRLSDYDVDHIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVP
                     SEEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKH
                     VAQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYL
                     NAVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKT
                     EITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSK
                     ESILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGI
                     TIMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNE
                     LALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILAD
                     ANLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVL
                     DATLIHQSITGLYETRIDLSQLGGD"
     CDS             2019..6122
                     /codon_start=1
                     /product="Cas9 (Csn1) endonuclease from the Streptococcus 
                     pyogenes Type II CRISPR/Cas system"
                     /label=Cas9
                     /note="generates RNA-guided double strand breaks in DNA"
                     /translation="MDKKYSIGLDIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKK
                     NLIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEES
                     FLVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIK
                     FRGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRL
                     ENLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQ
                     IGDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVR
                     QQLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLL
                     RKQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARG
                     NSRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEY
                     FTVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFD
                     SVEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEER
                     LKTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFM
                     QLIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGR
                     HKPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLY
                     LYYLQNGRDMYVDQELDINRLSDYDVDHIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVP
                     SEEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKH
                     VAQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYL
                     NAVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKT
                     EITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSK
                     ESILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGI
                     TIMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNE
                     LALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILAD
                     ANLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVL
                     DATLIHQSITGLYETRIDLSQLGGD"
     RBS             6035..6043
                     /label=Shine-Dalgarno sequence
                     /note="full consensus sequence for ribosome-binding sites 
                     upstream of start codons in E. coli; complementary to a 
                     region in the 3' end of the 16S rRNA (Chen et al., 1994)"
     CDS             6123..6170
                     /codon_start=1
                     /product="bipartite nuclear localization signal from 
                     nucleoplasmin"
                     /label=nucleoplasmin NLS
                     /translation="KRPAATKKAGQAKKKK"
     CDS             8343..8363
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     terminator      8372..8620
                     /label=HSP terminator
                     /note="efficient transcription terminator from the 
                     Arabidopsis thaliana heat shock protein 18.2 gene (Nagaya 
                     et al., 2010)"
     CDS             complement(8882..9187)
                     /codon_start=1
                     /gene="ccdB"
                     /product="CcdB, a bacterial toxin that poisons DNA gyrase"
                     /label=ccdB
                     /note="Plasmids containing the ccdB gene cannot be 
                     propagated in standard E. coli strains."
                     /translation="MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDK
                     VSRELYPVVHIGDESWRMMTTDMASVPVSVIGEEVADLSHRENDIKNAINLMFWGI"
     primer_bind     complement(8984..9003)
                     /label=ccdB-fwd
                     /note="ccdB gene, forward primer"
     protein_bind    complement(9518..9542)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     misc_feature    9572..9596
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     CDS             10897..11526
                     /codon_start=1
                     /product="stability protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 StaA
                     /translation="MKVIAVLNQKGGSGKTTIATHLARALQLAGADVLLVDSDPQGSAR
                     DWAAVREDQPLTVVGIDRPTIDRDVKAIGRRDFVVIDGAPQAADLAVSAIKAADFVLIP
                     VQPSPYDIWATADLVELVKQRIEVTDGRLQAAFVVSRAIKGTRIGGEVAEALAGYELPI
                     LESRITQRVSYPGTAAAGTTVLESEPEGDAAREVQALAAEIKSKLI"
     CDS             11955..13028
                     /codon_start=1
                     /product="replication protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 RepA
                     /translation="MSGRKPSGPVQIGAALGDDLVEKLKAAQAAQRQRIEAEARPGESW
                     QAAADRIRKESRQPPAAGAPSIRKPPKGDEQPDFFVPMLYDVGTRDSRSIMDVAVFRLS
                     KRDRRAGEVIRYELPDGHVEVSAGPAGMASVWDYDLVLMAVSHLTESMNRYREGKGDKP
                     GRVFRPHVADVLKFCRRADGGKQKDDLVETCIRLNTTHVAMQRTKKAKNGRLVTVSEGE
                     ALISRYKIVKSETGRPEYIEIELADWMYREITEGKNPDVLTVHPDYFLIDPGIGRFLYR
                     LARRAAGKAEARWLFKTIYERSGSAGEFKKFCFTVRKLIGSNDLPEYDLKEEAGQAGPI
                     LVMRYRNLIEGEASAGS"
     rep_origin      13094..13288
                     /label=pVS1 oriV
                     /note="origin of replication for the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
     primer_bind     complement(13524..13546)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     misc_feature    13622..13762
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     13677..13696
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     complement(13777..13794)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(13948..14536)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(14028..14047)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(15961..16755)
                     /codon_start=1
                     /gene="aphA-3"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin"
                     /translation="MAKMRISPELKKLIEKYRCVKDTEGMSPAKVYKLVGENENLYLKM
                     TDSRYKGTTYDVEREKDMMLWLEGKLPVPKVLHFERHDGWSNLLMSEADGVLCSEEYED
                     EQSPEKIIELYAECIRLFHSIDISDCPYTNSLDSRLAELDYLLNNDLADVDCENWEEDT
                     PFKDPRELYDFLKTEKPEEELVFSHGDLGDSNIFVKDGKVSGFIDLGRSGRADKWYDIA
                     FCVRSIREDIGEEQYVELFFDLLGIKPDWEKIKYYILLDELF"
     misc_feature    17180..17204
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     polyA_signal    17282..17456
                     /label=CaMV poly(A) signal
                     /note="cauliflower mosaic virus polyadenylation signal"
     promoter        complement(18546..19222)
                     /label=CaMV 35S promoter (enhanced)
                     /note="cauliflower mosaic virus 35S promoter with a 
                     duplicated enhancer region"
     primer_bind     complement(18589..18609)
                     /label=35S promoter
                     /note="CaMV 35S promoter, forward primer"
ORIGIN
        1 ggcgcgcctg cagtgcagcg tgacccggtc gtgcccctct cttgagataa ggtgagcatt
       61 gcatgtctaa gttataaaaa attaccacat attttttttg tcacacttgt ttgaagtgca
      121 gtttatctat ctttatacat atatttaaac tttactctac gaataatata atctatagta
      181 ctacaataat atcagtgttt tagagaatca tataaatgaa cagttagaca tggtctaaag
      241 gacaattgag tattttgaca acaggactct acagttttat ctttttagtg tgcatgtgtt
      301 ctcctttttt tttgcaaata gcttcaccta tataatactt catccatttt attagtacat
      361 ccatttaggg tttagggtta atggttttta tagactaatt tttttagtac atctatttta
      421 ttctatttta gcctctaaat taagaaaact aaaactctat tttagttttt ttatttaata
      481 atttagatat aaaatagaat aaaataaagt gactaaaaat taaacaaata ccctttaaga
      541 aattaaaaaa actaaggaaa catttttctt gtttcgagta gataatgcca gcctgttaaa
      601 cgccgtcgac gagtctaacg gacaccaacc agcgaaccag cagcgtcgcg tcgggccaag
      661 cgaagcagac ggcacggcat ctctgtcgct gcctctggac ccctctcgag agttccgctc
      721 caccgttgga cttgctccgc tgtcggcatc cagaaattgc gtggcggagc ggcagacgtg
      781 agccggcacg gcaggcggcc tcctcctcct ctcacggcac cggcagctac gggggattcc
      841 tttcccaccg ctccttcgct ttcccttcct cgcccgccgt aataaataga caccccctcc
      901 acaccctctt tccccaacct cgtgttgttc ggagcgcaca cacacacaac cagaactccc
      961 ccaaatccac ccgtcggcac ctccgcttca aggtacgccg ctcgtcctcc cccccccccc
     1021 ctctctacct tctcaagatc ggcgttccgg tccatggtta gggcccggta gttctacttc
     1081 tgttcatgtt tgtgttagat ccgtgtttgt gttagatccg tgctactagc gttcgtacac
     1141 ggatgcgacc tgtacgtcag acacgttctg attgctaact tgccagtgtt tctctttggg
     1201 gaatcctggg atggctctag ccgttccgca gacgggatcg atttcatgat tttttttgtt
     1261 tcgttgcata gggtttggtt tgcccttttc ctttatttca atatatgccg tgcacttgtt
     1321 tgtcgggtca tcttttcatg cttttttttg tcttggttgt gatgatgtgg tctggttggg
     1381 cggtcgttca agatcggagt agaattaatt ctgtttcaaa ctacctggtg gatttattaa
     1441 ttttggatct gtatgtgtgt gccatacata ttcatagtta cgaattgaag atgatggatg
     1501 gaaatatcga tctaggatag gtatacatgt tgatgcgggt tttactgatg catatacaga
     1561 gatgcttttt gttcgcttgg ttgtgatgat gtggtgtggt tgggcggtcg ttcattcgtt
     1621 caagatcgga gtagaatact gtttcaaact acctggtgta tttattaatt ttggaactgt
     1681 atgtgtgtgt catacatctt catagttacg agtttaagat ggatggaaat atcgatctag
     1741 gataggtata catgttgatg tgggttttac tgatgcatat acatgatggc atatgcagca
     1801 tctattcata tgctctaacc ttgagtacct atctattata ataaacaagt atgttttata
     1861 attattttga tcttgatata cttggatgat ggcatatgca gcagctatat gtggattttt
     1921 ttagccctgc cttcatacgc tatttatttg cttggtactg tttcttttgt cgatgctcac
     1981 cctgttgttt ggtgttactt ctgcagcctg caggccatat ggacaagaag tactcgatcg
     2041 gcctcgatat tgggactaac tctgttggct gggccgtgat caccgacgag tacaaggtgc
     2101 cctcaaagaa gttcaaggtc ctgggcaaca ccgatcggca ttccatcaag aagaatctca
     2161 ttggcgctct cctgttcgac agcggcgaga cggctgaggc tacgcggctc aagcgcaccg
     2221 cccgcaggcg gtacacgcgc aggaagaatc gcatctgcta cctgcaggag attttctcca
     2281 acgagatggc gaaggttgac gattctttct tccacaggct ggaggagtca ttcctcgtgg
     2341 aggaggataa gaagcacgag cggcatccaa tcttcggcaa cattgtcgac gaggttgcct
     2401 accacgagaa gtaccctacg atctaccatc tgcggaagaa gctcgtggac tccacagata
     2461 aggcggacct ccgcctgatc tacctcgctc tggcccacat gattaagttc aggggccatt
     2521 tcctgatcga gggggatctc aacccggaca atagcgatgt tgacaagctg ttcatccagc
     2581 tcgtgcagac gtacaaccag ctcttcgagg agaaccccat taatgcgtca ggcgtcgacg
     2641 cgaaggctat cctgtccgct aggctctcga agtctcggcg cctcgagaac ctgatcgccc
     2701 agctgccggg cgagaagaag aacggcctgt tcgggaatct cattgcgctc agcctggggc
     2761 tcacgcccaa cttcaagtcg aatttcgatc tcgctgagga cgccaagctg cagctctcca
     2821 aggacacata cgacgatgac ctggataacc tcctggccca gatcggcgat cagtacgcgg
     2881 acctgttcct cgctgccaag aatctgtcgg acgccatcct cctgtctgat attctcaggg
     2941 tgaacaccga gattacgaag gctccgctct cagcctccat gatcaagcgc tacgacgagc
     3001 accatcagga tctgaccctc ctgaaggcgc tggtcaggca gcagctcccc gagaagtaca
     3061 aggagatctt cttcgatcag tcgaagaacg gctacgctgg gtacattgac ggcggggcct
     3121 ctcaggagga gttctacaag ttcatcaagc cgattctgga gaagatggac ggcacggagg
     3181 agctgctggt gaagctcaat cgcgaggacc tcctgaggaa gcagcggaca ttcgataacg
     3241 gcagcatccc acaccagatt catctcgggg agctgcacgc tatcctgagg aggcaggagg
     3301 acttctaccc tttcctcaag gataaccgcg agaagatcga gaagattctg actttcagga
     3361 tcccgtacta cgtcggccca ctcgctaggg gcaactcccg cttcgcttgg atgacccgca
     3421 agtcagagga gacgatcacg ccgtggaact tcgaggaggt ggtcgacaag ggcgctagcg
     3481 ctcagtcgtt catcgagagg atgacgaatt tcgacaagaa cctgccaaat gagaaggtgc
     3541 tccctaagca ctcgctcctg tacgagtact tcacagtcta caacgagctg actaaggtga
     3601 agtatgtgac cgagggcatg aggaagccgg ctttcctgtc tggggagcag aagaaggcca
     3661 tcgtggacct cctgttcaag accaaccgga aggtcacggt taagcagctc aaggaggact
     3721 acttcaagaa gattgagtgc ttcgattcgg tcgagatctc tggcgttgag gaccgcttca
     3781 acgcctccct ggggacctac cacgatctcc tgaagatcat taaggataag gacttcctgg
     3841 acaacgagga gaatgaggat atcctcgagg acattgtgct gacactcact ctgttcgagg
     3901 accgggagat gatcgaggag cgcctgaaga cttacgccca tctcttcgat gacaaggtca
     3961 tgaagcagct caagaggagg aggtacaccg gctgggggag gctgagcagg aagctcatca
     4021 acggcattcg ggacaagcag tccgggaaga cgatcctcga cttcctgaag agcgatggct
     4081 tcgcgaaccg caatttcatg cagctgattc acgatgacag cctcacattc aaggaggata
     4141 tccagaaggc tcaggtgagc ggccaggggg actcgctgca cgagcatatc gcgaacctcg
     4201 ctggctcgcc agctatcaag aaggggattc tgcagaccgt gaaggttgtg gacgagctgg
     4261 tgaaggtcat gggcaggcac aagcctgaga acatcgtcat tgagatggcc cgggagaatc
     4321 agaccacgca gaagggccag aagaactcac gcgagaggat gaagaggatc gaggagggca
     4381 ttaaggagct ggggtcccag atcctcaagg agcacccggt ggagaacacg cagctgcaga
     4441 atgagaagct ctacctgtac tacctccaga atggccgcga tatgtatgtg gaccaggagc
     4501 tggatattaa caggctcagc gattacgacg tcgatcatat cgttccacag tcattcctga
     4561 aggatgactc cattgacaac aaggtcctca ccaggtcgga caagaaccgg ggcaagtctg
     4621 ataatgttcc ttcagaggag gtcgttaaga agatgaagaa ctactggcgc cagctcctga
     4681 atgccaagct gatcacgcag cggaagttcg ataacctcac aaaggctgag aggggcgggc
     4741 tctctgagct ggacaaggcg ggcttcatca agaggcagct ggtcgagaca cggcagatca
     4801 ctaagcacgt tgcgcagatt ctcgactcac ggatgaacac taagtacgat gagaatgaca
     4861 agctgatccg cgaggtgaag gtcatcaccc tgaagtcaaa gctcgtctcc gacttcagga
     4921 aggatttcca gttctacaag gttcgggaga tcaacaatta ccaccatgcc catgacgcgt
     4981 acctgaacgc ggtggtcggc acagctctga tcaagaagta cccaaagctc gagagcgagt
     5041 tcgtgtacgg ggactacaag gtttacgatg tgaggaagat gatcgccaag tcggagcagg
     5101 agattggcaa ggctaccgcc aagtacttct tctactctaa cattatgaat ttcttcaaga
     5161 cagagatcac tctggccaat ggcgagatcc ggaagcgccc cctcatcgag acgaacggcg
     5221 agacggggga gatcgtgtgg gacaagggca gggatttcgc gaccgtcagg aaggttctct
     5281 ccatgccaca agtgaatatc gtcaagaaga cagaggtcca gactggcggg ttctctaagg
     5341 agtcaattct gcctaagcgg aacagcgaca agctcatcgc ccgcaagaag gactgggatc
     5401 cgaagaagta cggcgggttc gacagcccca ctgtggccta ctcggtcctg gttgtggcga
     5461 aggttgagaa gggcaagtcc aagaagctca agagcgtgaa ggagctgctg gggatcacga
     5521 ttatggagcg ctccagcttc gagaagaacc cgatcgattt cctggaggcg aagggctaca
     5581 aggaggtgaa gaaggacctg atcattaagc tccccaagta ctcactcttc gagctggaga
     5641 acggcaggaa gcggatgctg gcttccgctg gcgagctgca gaaggggaac gagctggctc
     5701 tgccgtccaa gtatgtgaac ttcctctacc tggcctccca ctacgagaag ctcaagggca
     5761 gccccgagga caacgagcag aagcagctgt tcgtcgagca gcacaagcat tacctcgacg
     5821 agatcattga gcagatttcc gagttctcca agcgcgtgat cctggccgac gcgaatctgg
     5881 ataaggtcct ctccgcgtac aacaagcacc gcgacaagcc aatcagggag caggctgaga
     5941 atatcattca tctcttcacc ctgacgaacc tcggcgcccc tgctgctttc aagtacttcg
     6001 acacaactat cgatcgcaag aggtacacaa gcactaagga ggtcctggac gcgaccctca
     6061 tccaccagtc gattaccggc ctctacgaga cgcgcatcga cctgtctcag ctcgggggcg
     6121 acaagcggcc agcggcgacg aagaaggcgg ggcaggcgaa gaagaagaag tcagggggtt
     6181 ctagcggcgg ttcatctggc tccgaaacac cgggaacttc cgagagtgct actccagaat
     6241 ctagcggtgg gtcatctgga gggtcttcca cacttaatat tgaggacgaa taccgcctgc
     6301 atgaaactag taaagaaccc gacgtgagcc tcggtagcac gtggctctct gatttcccgc
     6361 aagcatgggc tgaaacaggt gggatgggcc tcgctgttcg ccaggctcca ttgatcatcc
     6421 cactcaaggc cacttctaca ccggtgtcca taaagcaata cccaatgagc caggaagctc
     6481 ggcttggtat caagcctcac atccaaagat tgcttgatca gggaattctt gtcccttgtc
     6541 aaagtccatg gaacacccct cttcttcccg tcaaaaaacc tgggactaac gattataggc
     6601 cagtgcaaga cctcagggag gttaacaaaa gggttgaaga cattcatcca acagtgccga
     6661 atccttataa tctgctctca ggtttgcctc caagtcacca gtggtataca gtgctggatc
     6721 ttaaagacgc atttttttgt ctgcgccttc atcccacttc gcaacctctt tttgctttcg
     6781 aatggagaga tccagagatg ggtatctccg gacagctcac atggacccgg ttgccccaag
     6841 gcttcaaaaa ctccccaacc ttgtttaatg aagcgctgca cagagatttg gcggacttca
     6901 ggatccagca ccccgacttg attctcctgc agtatgtgga tgaccttttg cttgcagcca
     6961 catctgagct tgactgccaa caggggacgc gcgcccttct tcagactttg ggaaaccttg
     7021 ggtatagagc ctctgccaag aaggcacaga tctgcccgaa gcaagttaag tatctcggtt
     7081 atttgctcaa ggagggtcag aggtggctga cggaggcgag gaaagaaacg gtgatgggcc
     7141 aaccaacccc aaaaacccct aggcaactta gagagtttct tggtaaagcg gggttttgcc
     7201 ggttgttcat tccaggcttt gccgagatgg ccgcaccgtt gtatccattg acgaaaccgg
     7261 gcaccctttt taattggggg cccgaccagc agaaggcgta tcaggagatc aagcaagctc
     7321 tcctgaccgc accagcactc ggacttccag atcttacgaa accgtttgag ctcttcgtgg
     7381 atgagaaaca ggggtatgct aagggagtcc tcacacaaaa gttgggaccg tggaggcggc
     7441 cggttgccta tctttcgaaa aaacttgatc ccgtggcggc tggttggcct ccctgtctga
     7501 ggatggtggc cgcgatcgca gtccttacaa aagatgcagg gaagttgact atgggacaac
     7561 cattggtcat acttgccccg cacgcggttg aggccctcgt taagcagcct cccgataggt
     7621 ggctcagtaa cgcccgcatg actcattacc aggcactgct tctcgacaca gacagggtgc
     7681 agtttggtcc cgtcgtcgct ttgaaccccg ccaccctgct gcccctcccg gaggaaggat
     7741 tgcagcacaa ctgtctggat atcttggccg aagcgcatgg gactcgcccg gacttgactg
     7801 accagcccct tcccgacgct gaccatactt ggtatacgga tggaagcagc cttctgcagg
     7861 aagggcaacg caaggcaggg gccgcggtta ctactgaaac tgaggtcatc tgggctaagg
     7921 ctttgccagc cggaactagt gctcaaagag cagaattgat tgccctcacg caagctttga
     7981 aaatggctga gggtaagaag cttaatgtgt atacagacag taggtatgca tttgcgacag
     8041 cacatatcca tggtgagatc taccggcgca gaggatggct tacgagcgag gggaaggaga
     8101 ttaagaacaa agatgagatt ttggccctgc tcaaagcgct cttcctcccg aagcgcctta
     8161 gcatcatcca ttgcccaggc catcagaagg gtcactcggc cgaagcacgg ggtaatagga
     8221 tggccgatca ggcagcaagg aaggctgcta tcactgagac acccgacacc tctacccttc
     8281 tcatagagaa ttcatcgccg agcggcggca gcaagaggac tgcagacggg tcggaattcg
     8341 aaccgaagaa aaagagaaag gtgtgagctt atatgaagat gaagatgaaa tatttggtgt
     8401 gtcaaataaa aagcttgtgt gcttaagttt gtgttttttt cttggcttgt tgtgttatga
     8461 atttgtggct ttttctaata ttaaatgaat gtaagatcac attataatga ataaacaaat
     8521 gtttctataa tccattgtga atgttttgtt ggatctcttc tgcagcatat aactactgta
     8581 tgtgctatgg tatggactat ggaatatgat taaagataag aggatccgcg gatcatgaac
     8641 caacggcctg gctgtatttg gtggttgtgt agggagatgg ggagaagaaa agcccgattc
     8701 tcttcgctgt gatgggctgg atgcatgcgg gggagcggga ggcccaagta cgtgcacggt
     8761 gagcggccca cagggcgagt gtgagcgcga gaggcgggag gaacagttta gtaccacatt
     8821 gcccagctaa ctcgaacgcg accaacttat aaacccgcgc gctgtcgctt gtgtggagac
     8881 cttatattcc ccagaacatc aggttaatgg cgtttttgat gtcattttcg cggtggctga
     8941 gatcagccac ttcttccccg ataacggaaa ccggcacact ggccatatcg gtggtcatca
     9001 tgcgccagct ttcatccccg atatgcacca ccgggtaaag ttcacgggag actttatctg
     9061 acagcagacg tgcactggcc agggggatca ccatccgtcg cccgggcgtg tcaataatat
     9121 cactctgtac atccacaaac agacgataac ggctctctct tttataggtg taaaccttaa
     9181 actgcatttc accagcccct gttctcgtca gcaaaagagc cgttcatttc aataaaccgg
     9241 gcgacctcag ccatcccttc ctgattttcc gctttccagc gttcggcacg cagacgacgg
     9301 gcttcattct gcatggttgt gcttaccaga ccggagatat tgacatcata tatgccttga
     9361 gcaactgata gctgtcgctg tcaactgtca ctgtaatacg ctgcttcata gcatacctct
     9421 ttttgacata cttcgggtat acatatcagt atatattctt ataccgcaaa aatcagcgcg
     9481 caaatacgca tactgttatc tggcttggtc tctctagacc cagctttctt gtacaaagtt
     9541 ggcattactg cgagctcaaa ctatcagtgt ttgacaggat atattggcgg gtaaacctaa
     9601 gagaaaagag cgtttattag aataatcgga tatttaaaag ggcgtgaaaa ggtttatccg
     9661 ttcgtccatt tgtatgtgca tgccaaccac agggttcccc tcgggatcaa agtactttga
     9721 tccaacccct ccgctgctat agtgcagtcg gcttctgacg ttcagtgcag ccgtcttctg
     9781 aaaacgacat gtcgcacaag tcctaagtta cgcgacaggc tgccgccctg cccttttcct
     9841 ggcgttttct tgtcgcgtgt tttagtcgca taaagtagaa tacttgcgac tagaaccgga
     9901 gacattacgc catgaacaag agcgccgccg ctggcctgct gggctatgcc cgcgtcagca
     9961 ccgacgacca ggacttgacc aaccaacggg ccgaactgca cgcggccggc tgcaccaagc
    10021 tgttttccga gaagatcacc ggcaccaggc gcgaccgccc ggagctggcc aggatgcttg
    10081 accacctacg ccctggcgac gttgtgacag tgaccaggct agaccgcctg gcccgcagca
    10141 cccgcgacct actggacatt gccgagcgca tccaggaggc cggcgcgggc ctgcgtagcc
    10201 tggcagagcc gtgggccgac accaccacgc cggccggccg catggtgttg accgtgttcg
    10261 ccggcattgc cgagttcgag cgttccctaa tcatcgaccg cacccggagc gggcgcgagg
    10321 ccgccaaggc ccgaggcgtg aagtttggcc cccgccctac cctcaccccg gcacagatcg
    10381 cgcacgcccg cgagctgatc gaccaggaag gccgcaccgt gaaagaggcg gctgcactgc
    10441 ttggcgtgca tcgctcgacc ctgtaccgcg cacttgagcg cagcgaggaa gtgacgccca
    10501 ccgaggccag gcggcgcggt gccttccgtg aggacgcatt gaccgaggcc gacgccctgg
    10561 cggccgccga gaatgaacgc caagaggaac aagcatgaaa ccgcaccagg acggccagga
    10621 cgaaccgttt ttcattaccg aagagatcga ggcggagatg atcgcggccg ggtacgtgtt
    10681 cgagccgccc gcgcacggct caaccgtgcg gctgcatgaa atcctggccg gtttgtctga
    10741 tgccaagctg gcggcctggc cggccagctt ggccgctgaa gaaaccgagc gccgccgtct
    10801 aaaaaggtga tgtgtatttg agtaaaacag cttgcgtcat gcggtcgctg cgtatatgat
    10861 gcgatgagta aataaacaaa tacgcaaggg gaacgcatga aggttatcgc tgtacttaac
    10921 cagaaaggcg ggtcaggcaa gacgaccatc gcaacccatc tagcccgcgc cctgcaactc
    10981 gccggggccg atgttctgtt agtcgattcc gatccccagg gcagtgcccg cgattgggcg
    11041 gccgtgcggg aagatcaacc gctaaccgtt gtcggcatcg accgcccgac gattgaccgc
    11101 gacgtgaagg ccatcggccg gcgcgacttc gtagtgatcg acggagcgcc ccaggcggcg
    11161 gacttggctg tgtccgcgat caaggcagcc gacttcgtgc tgattccggt gcagccaagc
    11221 ccttacgaca tatgggccac cgccgacctg gtggagctgg ttaagcagcg cattgaggtc
    11281 acggatggaa ggctacaagc ggcctttgtc gtgtcgcggg cgatcaaagg cacgcgcatc
    11341 ggcggtgagg ttgccgaggc gctggccggg tacgagctgc ccattcttga gtcccgtatc
    11401 acgcagcgcg tgagctaccc aggcactgcc gccgccggca caaccgttct tgaatcagaa
    11461 cccgagggcg acgctgcccg cgaggtccag gcgctggccg ctgaaattaa atcaaaactc
    11521 atttgagtta atgaggtaaa gagaaaatga gcaaaagcac aaacacgcta agtgccggcc
    11581 gtccgagcgc acgcagcagc aaggctgcaa cgttggccag cctggcagac acgccagcca
    11641 tgaagcgggt caactttcag ttgccggcgg aggatcacac caagctgaag atgtacgcgg
    11701 tacgccaagg caagaccatt accgagctgc tatctgaata catcgcgcag ctaccagagt
    11761 aaatgagcaa atgaataaat gagtagatga attttagcgg ctaaaggagg cggcatggaa
    11821 aatcaagaac aaccaggcac cgacgccgtg gaatgcccca tgtgtggagg aacgggcggt
    11881 tggccaggcg taagcggctg ggttgcctgc cggccctgca atggcactgg aacccccaag
    11941 cccgaggaat cggcgtgagc ggtcgcaaac catccggccc ggtacaaatc ggcgcggcgc
    12001 tgggtgatga cctggtggag aagttgaagg ccgcgcaggc cgcccagcgg caacgcatcg
    12061 aggcagaagc acgccccggt gaatcgtggc aagcggccgc tgatcgaatc cgcaaagaat
    12121 cccggcaacc gccggcagcc ggtgcgccgt cgattaggaa gccgcccaag ggcgacgagc
    12181 aaccagattt tttcgttccg atgctctatg acgtgggcac ccgcgatagt cgcagcatca
    12241 tggacgtggc cgttttccgt ctgtcgaagc gtgaccgacg agctggcgag gtgatccgct
    12301 acgagcttcc agacgggcac gtagaggttt ccgcagggcc ggccggcatg gccagtgtgt
    12361 gggattacga cctggtactg atggcggttt cccatctaac cgaatccatg aaccgatacc
    12421 gggaagggaa gggagacaag cccggccgcg tgttccgtcc acacgttgcg gacgtactca
    12481 agttctgccg gcgagccgat ggcggaaagc agaaagacga cctggtagaa acctgcattc
    12541 ggttaaacac cacgcacgtt gccatgcagc gtacgaagaa ggccaagaac ggccgcctgg
    12601 tgacggtatc cgagggtgaa gccttgatta gccgctacaa gatcgtaaag agcgaaaccg
    12661 ggcggccgga gtacatcgag atcgagctag ctgattggat gtaccgcgag atcacagaag
    12721 gcaagaaccc ggacgtgctg acggttcacc ccgattactt tttgatcgat cccggcatcg
    12781 gccgttttct ctaccgcctg gcacgccgcg ccgcaggcaa ggcagaagcc agatggttgt
    12841 tcaagacgat ctacgaacgc agtggcagcg ccggagagtt caagaagttc tgtttcaccg
    12901 tgcgcaagct gatcgggtca aatgacctgc cggagtacga tttgaaggag gaggcggggc
    12961 aggctggccc gatcctagtc atgcgctacc gcaacctgat cgagggcgaa gcatccgccg
    13021 gttcctaatg tacggagcag atgctagggc aaattgccct agcaggggaa aaaggtcgaa
    13081 aaggcctctt tcctgtggat agcacgtaca ttgggaaccc aaagccgtac attgggaacc
    13141 ggaacccgta cattgggaac ccaaagccgt acattgggaa ccggtcacac atgtaagtga
    13201 ctgatataaa agagaaaaaa ggcgattttt ccgcctaaaa ctctttaaaa cttattaaaa
    13261 ctcttaaaac ccgcctggcc tgtgcataac tgtctggcca gcgcacagcc gaagagctgc
    13321 aaaaagcgcc tacccttcgg tcgctgcgct ccctacgccc cgccgcttcg cgtcggccta
    13381 tcgcggccgc tggccgctca aaaatggctg gcctacggcc aggcaatcta ccagggcgcg
    13441 gacaagccgc gccgtcgcca ctcgaccgcc ggcgcccaca tcaaggcacc ctgcctcgcg
    13501 cgtttcggtg atgacggtga aaacctctga cacatgcagc tcccggaaac ggtcacagct
    13561 tgtctgtaag cggatgccgg gagcagacaa gcgcgtcagc gggtgttggc gggtgtcggg
    13621 gcgcagccat gacccagtca cgtagcgata gcggagtgta tactggctta actatgcggc
    13681 atcagagcag attgtactga gagtgcacca tatgcggtgt gaaataccgc acagatgcgt
    13741 aaggagaaaa taccgcatca ggcgctcttc cgcttcctcg ctcactgact cgctgcgctc
    13801 ggtcgttcgg ctgcggcgag cggtatcagc tcactcaaag gcggtaatac ggttatccac
    13861 agaatcaggg gataacgcag gaaagaacat gtgagcaaaa ggccagcaaa aggccaggaa
    13921 ccgtaaaaag gccgcgttgc tggcgttttt ccataggctc cgcccccctg acgagcatca
    13981 caaaaatcga cgctcaagtc agaggtggcg aaacccgaca ggactataaa gataccaggc
    14041 gtttccccct ggaagctccc tcgtgcgctc tcctgttccg accctgccgc ttaccggata
    14101 cctgtccgcc tttctccctt cgggaagcgt ggcgctttct catagctcac gctgtaggta
    14161 tctcagttcg gtgtaggtcg ttcgctccaa gctgggctgt gtgcacgaac cccccgttca
    14221 gcccgaccgc tgcgccttat ccggtaacta tcgtcttgag tccaacccgg taagacacga
    14281 cttatcgcca ctggcagcag ccactggtaa caggattagc agagcgaggt atgtaggcgg
    14341 tgctacagag ttcttgaagt ggtggcctaa ctacggctac actagaagga cagtatttgg
    14401 tatctgcgct ctgctgaagc cagttacctt cggaaaaaga gttggtagct cttgatccgg
    14461 caaacaaacc accgctggta gcggtggttt ttttgtttgc aagcagcaga ttacgcgcag
    14521 aaaaaaagga tctcaagaag atcctttgat cttttctacg gggtctgacg ctcagtggaa
    14581 cgaaaactca cgttaagggc tgatgaatcc cctaatgatt tttatcaaaa tcattaagtt
    14641 aaggtagata cacatcttgt catatgatca aatggtttcg ccaaaaatca ataatcagac
    14701 aacaaaatgt gcgaactcga tattttacac gactctcttt accaattctg ccccgaatta
    14761 cacttaaaac gactcaacag cttaacgttg gcttgccacg ccttacttga ctgtaaaact
    14821 ctcactctta ccgaacttgg ccgtaacctg ccaaccaaag cgagaacaaa acataacatc
    14881 aaacgaatcg accgattgtt aggtaatcgt cacctccaca aagagcgact cgctgtatac
    14941 cgttggcatg ctagctttat ctgttcgggc aatacgatgc ccattgtact tgttgactgg
    15001 tctgatatcc gtgagcaaaa acggcttatg gtattgcgag cttcagtcgc actacacggt
    15061 cgttctgtta ctctttatga gaaagcgttc ccgctttcag agcaatgttc aaagaaagct
    15121 catgaccaat ttctagccga ccttgcgagc attctaccga gtaacaccac accgctcatt
    15181 gtcagtgatg ctggctttaa agtgccatgg tataaatccg ttgagaagct gggttggtac
    15241 tggttaagtc gagtaagagg aaaagtacaa tatgcagacc taggagcgga aaactggaaa
    15301 cctatcagca acttacatga tatgtcatct agtcactcaa agactttagg ctataagagg
    15361 ctgactaaaa gcaatccaat ctcatgccaa attctattgt ataaatctcg ctctaaaggc
    15421 cgaaaaaatc agcgctcgac acggactcat tgtcaccacc cgtcacctaa aatctactca
    15481 gcgtcggcaa aggagccatg gattctagca actaacttac ctgttgaaat tcgaacaccc
    15541 aaacaacttg ttaatatcta ttcgaagcga atgcagattg aagaaacctt ccgagacttg
    15601 aaaagtcctg cctacggact aggcctacgc catagccgaa cgagcagctc agagcgtttt
    15661 gatatcatgc tgctaatcgc cctgatgctt caactaacat gttggcttgc gggcgttcat
    15721 gctcagaaac aaggttggga caagcacttc caggctaaca cagtcagaaa tcgaaacgta
    15781 ctctcaacag ttcgcttagg catggaagtt ttgcggcatt ctggctacac aataacaagg
    15841 gaagacttac tcgtggctgc aaccctacta gctcaaaatt tattcacaca tggttacgct
    15901 ttggggaaat tatgagggga tctctcagcg ttaagggatt ttggtcatgc attctaggta
    15961 ctaaaacaat tcatccagta aaatataata ttttattttc tcccaatcag gcttgatccc
    16021 cagtaagtca aaaaatagct cgacatactg ttcttccccg atatcctccc tgatcgaccg
    16081 gacgcagaag gcaatgtcat accacttgtc cgccctgccg cttctcccaa gatcaataaa
    16141 gccacttact ttgccatctt tcacaaagat gttgctgtct cccaggtcgc cgtgggaaaa
    16201 gacaagttcc tcttcgggct tttccgtctt taaaaaatca tacagctcgc gcggatcttt
    16261 aaatggagtg tcttcttccc agttttcgca atccacatcg gccagatcgt tattcagtaa
    16321 gtaatccaat tcggctaagc ggctgtctaa gctattcgta tagggacaat ccgatatgtc
    16381 gatggagtga aagagcctga tgcactccgc atacagctcg ataatctttt cagggctttg
    16441 ttcatcttca tactcttccg agcaaaggac gccatcggcc tcactcatga gcagattgct
    16501 ccagccatca tgccgttcaa agtgcaggac ctttggaaca ggcagctttc cttccagcca
    16561 tagcatcatg tccttttccc gttccacatc ataggtggtc cctttatacc ggctgtccgt
    16621 catttttaaa tataggtttt cattttctcc caccagctta tataccttag caggagacat
    16681 tccttccgta tcttttacgc agcggtattt ttcgatcagt tttttcaatt ccggtgatat
    16741 tctcatttta gccatttatt atttccttcc tcttttctac agtatttaaa gataccccaa
    16801 gaagctaatt ataacaagac gaactccaat tcactgttcc ttgcattcta aaaccttaaa
    16861 taccagaaaa cagctttttc aaagttgttt tcaaagttgg cgtataacat agtatcgacg
    16921 gagccgattt tgaaaccgcg gtgatcacag gcagcaacgc tctgtcatcg ttacaatcaa
    16981 catgctaccc tccgcgagat catccgtgtt tcaaacccgg cagcttagtt gccgttcttc
    17041 cgaatagcat cggtaacatg agcaaagtct gccgccttac aacggctctc ccgctgacgc
    17101 cgtcccggac tgatgggctg cctgtatcga gtggtgattt tgtgccgagc tgccggtcgg
    17161 ggagctgttg gctggctggt ggcaggatat attgtggtgt aaacaaattg acgcttagac
    17221 aacttaataa cacattgcgg acgtttttaa tgtactgaat taacgccgaa ttaattcggg
    17281 ggatctggat tttagtactg gattttggtt ttaggaatta gaaattttat tgatagaagt
    17341 attttacaaa tacaaataca tactaagggt ttcttatatg ctcaacacat gagcgaaacc
    17401 ctataggaac cctaattccc ttatctggga actactcaca cattattatg gagaaaatag
    17461 agcttgtcga tcgttaatta atcacctcca agatcccgga cgagtgctgg ggcgtcggtt
    17521 tccactatcg gcgagtactt ctacacagcc atcggtccag acggccgcgc ttctgcgggc
    17581 gatttgtgta cgcccgacag tcccggctcc ggatcggacg attgcgtcgc atcgaccctg
    17641 cgcccaagct gcatcatcga aattgccgtc aaccaagctc tgatagagtt ggtcaagacc
    17701 aatgcggagc atatacgccc ggagccgcgg cgatcctgca agctccggat gcctccgctc
    17761 gaagtagcgc gtctgctgct ccatacaagc caaccacggc ctccagaaga agatgttggc
    17821 gacctcgtat tgggaatccc cgaacatcgc ctcgctccag tcaatgaccg ctgttatgcg
    17881 gccattgtcc gtcaggacat tgttggagcc gaaatccgcg tgcacgaggt gccggacttc
    17941 ggggcagtcc tcggcccaaa gcatcagctc atcgagagcc tgcgcgacgg acgcactgac
    18001 ggtgtcgtcc atcacagttt gccagtgata cacatgggga tcagcaatcg cgcatatgaa
    18061 atcacgccat gtagtgtatt gaccgattcc ttgcggtccg aatgggccga acccgctcgt
    18121 ctggctaaga tcggccgcag cgatcgcatc catggcctcc gcgaccggct gcagaacagc
    18181 gggcagttcg gtttcaggca ggtcttgcaa cgtgacaccc tgtgcacggc gggagatgca
    18241 ataggtcagg ctctcgctga attccccaat gtcaagcact tccggaatcg ggagcgcggc
    18301 cgatgcaaag tgccgataaa cataacgatc tttgtagaaa ccatcggcgc agctatttac
    18361 ccgcaggaca tatccacgcc ctcctacatc gaagctgaaa gcacgagatt cttcgccctc
    18421 cgagagctgc atcaggtcgg acacgctgtc gaacttttcg atcagaaact tctcgacaga
    18481 cgtcgcggtg agttcaggct ttttcatcct aggagagata gatttgtaga gagagactgg
    18541 tgatttcagc gtgtcctctc caaatgaaat gaacttcctt atatagagga agggtcttgc
    18601 gaaggatagt gggattgtgc gtcatccctt acgtcagtgg agatatcaca tcaatccact
    18661 tgctttgaag acgtggttgg aacgtcttct ttttccacga tgctcctcgt gggtgggggt
    18721 ccatctttgg gaccactgtc ggcagaggca tcttgaacga tagcctttcc tttatcgcaa
    18781 tgatggcatt tgtaggtgcc accttccttt tctactgtcc ttttgatgaa gtgacagata
    18841 gctgggcaat ggaatccgag gaggtttccc gatattaccc tttgttgaaa agtctcaata
    18901 gccctttggt cttctgagac tgtatctttg atattcttgg agtagacgag agtgtcgtgc
    18961 tccaccatgt tcacatcaat ccacttgctt tgaagacgtg gttggaacgt cttctttttc
    19021 cacgatgctc ctcgtgggtg ggggtccatc tttgggacca ctgtcggcag aggcatcttg
    19081 aacgatagcc tttcctttat cgcaatgatg gcatttgtag gtgccacctt ccttttctac
    19141 tgtccttttg atgaagtgac agatagctgg gcaatggaat ccgaggaggt ttcccgatat
    19201 taccctttgt tgaaaagtct caatagccct ttggtcttct gagactgtat ctttgatatt
    19261 cttggagtag acgagagtgt cgtgctccac catg
//
