LOCUS       sequence-410945-       11398 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Mammalian expression of hGBF1.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11398)
  AUTHORS   Kawamoto K, Yoshida Y, Tamaki H, Torii S, Shinotsuka C, Yamashina S,
            Nakayama K
  TITLE     GBF1, a guanine nucleotide exchange factor for ADP-ribosylation 
            factors, is localized to the cis-Golgi and involved in membrane 
            association of the COPI coat.
  JOURNAL   Traffic. 2002 Jul;3(7):483-95. doi: 
            10.1034/j.1600-0854.2002.30705.x.
  PUBMED    12047556
REFERENCE   2  (bases 1 to 11398)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1034/j.1600-0854.2002.30705"; journalName: "Traffic"; date: 
            "2002-07"; volume: "3"; issue: "7"; pages: "483-95"
COMMENT     Sequence Label: pcDNA4-Xpress-GBF1
FEATURES             Location/Qualifiers
     source          1..11398
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(48..67)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        239..618
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        619..822
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     773..793
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     867..886
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        867..885
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     misc_feature    921..1083
                     /label=QBI SP163
                     /note="strong translational enhancer derived from the 5' 
                     UTR of the mouse VEGF gene"
     CDS             1096..1113
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             1117..1149
                     /codon_start=1
                     /product="leader peptide from bacteriophage T7 gene 10"
                     /label=T7 tag (gene 10 leader)
                     /note="promotes efficient translation in E. coli"
                     /translation="MASMTGGQQMG"
     CDS             join(1153..1161,1162..1176)
                     /codon_start=1
                     /product="Xpress(TM) epitope tag, including an enterokinase
                     recognition and cleavage site"
                     /label=Xpress(TM) tag
                     /translation="DLYDDDDK"
     primer_bind     complement(7410..7427)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    7416..7640
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      7686..8114
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(7773..7792)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     7983..8004
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(8123..8143)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        8128..8457
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      8308..8443
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     8370..8389
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     promoter        8505..8552
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     CDS             8571..8945
                     /codon_start=1
                     /gene="Sh ble from Streptoalloteichus hindustanus"
                     /product="antibiotic-binding protein"
                     /label=BleoR
                     /note="confers resistance to bleomycin, phleomycin, and 
                     Zeocin(TM)"
                     /translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD
                     VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR
                     EFALRDPAGNCVHFVAEEQD"
     polyA_signal    9075..9196
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(9112..9131)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     9166..9185
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(9245..9261)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(9245..9261)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(9258..9280)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    9269..9285
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(9293..9299,9300..9317,9318..9323))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    9338..9359
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(9476..9493)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(9647..10235)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(9727..9746)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(10406..11197,11198..11266))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     11029..11048
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(11267..11371)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 cgtcgacgga tcgggagatc tcccgatccc ctatggtgca ctctcagtac aatctgctct
       61 gatgccgcat agttaagcca gtatctgctc cctgcttgtg tgttggaggt cgctgagtag
      121 tgcgcgagca aaatttaagc tacaacaagg caaggcttga ccgacaattg catgaagaat
      181 ctgcttaggg ttaggcgttt tgcgctgctt cgcgatgtac gggccagata tacgcgttga
      241 cattgattat tgactagtta ttaatagtaa tcaattacgg ggtcattagt tcatagccca
      301 tatatggagt tccgcgttac ataacttacg gtaaatggcc cgcctggctg accgcccaac
      361 gacccccgcc cattgacgtc aataatgacg tatgttccca tagtaacgcc aatagggact
      421 ttccattgac gtcaatgggt ggagtattta cggtaaactg cccacttggc agtacatcaa
      481 gtgtatcata tgccaagtac gccccctatt gacgtcaatg acggtaaatg gcccgcctgg
      541 cattatgccc agtacatgac cttatgggac tttcctactt ggcagtacat ctacgtatta
      601 gtcatcgcta ttaccatggt gatgcggttt tggcagtaca tcaatgggcg tggatagcgg
      661 tttgactcac ggggatttcc aagtctccac cccattgacg tcaatgggag tttgttttgg
      721 caccaaaatc aacgggactt tccaaaatgt cgtaacaact ccgccccatt gacgcaaatg
      781 ggcggtaggc gtgtacggtg ggaggtctat ataagcagag ctctctggct aactagagaa
      841 cccactgctt actggcttat cgaaattaat acgactcact atagggagac ccaagctggc
      901 tagcgtttaa acttaagctt agcgcagagg cttggggcag ccgagcggca gccaggcccc
      961 ggcccgggcc tcggttccag aagggagagg agcccgccaa ggcgcgcaag agagcgggct
     1021 gcctcgcagt ccgagccgga gagggagcgc gagccgcgcc ggccccggac ggcctccgaa
     1081 accatggggg gttctcatca tcatcatcat catggtatgg ctagcatgac tggtggacag
     1141 caaatgggtc gggatctgta cgacgatgac gataaggtac caggatctac catggtggat
     1201 aagaatattt acatcattca aggggagatt aacattgtgg ttggggccat caaacgaaat
     1261 gcccgatgga gcacccatac accactggat gaagaacggg atcctctgct gcatagtttc
     1321 ggtcatctaa aggaggtttt aaacagtata acagaactct cagaaattga gcccaatgta
     1381 ttccttcgac cttttctgga agtgattcgc tctgaagata ccactggccc tatcactgga
     1441 ctggcactca cctctgtcaa caagttcctg tcctatgcac tcatagatcc cacccatgag
     1501 ggcacagcag agggcatgga gaacatggca gatgctgtca cccatgctcg ttttgtgggc
     1561 acggatcctg ccagtgatga agttgtcctg atgaaaatcc ttcaggttct acggactctg
     1621 ctgctaaccc cagtgggtgc ccacctaacc aatgaatctg tgcgtgagat tatgcagtct
     1681 tgcttccgga tctgctttga aatgaggctc agtgagttat tgagaaaatc cgcagagcac
     1741 actctcgtag acatggtgca gctgctcttc acaaggttac ctcagtttaa agaagaaccc
     1801 aagaactatg tggggaccaa catgaagaag ctgaaaatga gagccggagg catgagtgat
     1861 tcatccaaat ggaagaaaca gaagagatcc cctcggcccc cacgccatat gaccaaagtc
     1921 acaccaggtt cagagctgcc cactcccaat ggaaccacct tatcatctaa cctcactggt
     1981 ggcatgccct tcattgatgt gcccactccc atctcctctg caagttcaga agctgcctca
     2041 gcagtggtca gtccctctac agacagtggc ctggaattct cctcccaaac cacttccaag
     2101 gaagacctta ctgatctaga gcaacctggc tctccagggt acagcacagc tacagagcct
     2161 ggaagcagtg agctaggtgt tcccgagcag cctgacctcc aggaagggac ccatgtggaa
     2221 aagtcccagt cagcatctgt ggagtccatc cctgaagtgt tagaggagtg cacgtcccct
     2281 gccgaccact ctgactctgc ctctgtccat gacatggatt acgtcaatcc ccggggcgtg
     2341 cgctttacac agtcctccca gaaagaaggc acagctttgg tcccctatgg tcttccctgc
     2401 atccgcgagc tcttccgctt cctcatctcc ctcaccaatc cacacgaccg ccataactca
     2461 gaggttatga ttcacatggg actgcatttg ctgacagtgg cccttgagtc agcccctgta
     2521 gcccagtgcc agaccctctt gggcctcatc aaggatgaga tgtgccgtca cttattccag
     2581 ctactcagca tagagcgact aaacctttat gctgcttccc tgcgagtatg cttcctactg
     2641 tttgagagca tgcgagagca cctcaagttc caaatggaga tgtacatcaa aaagcttatg
     2701 gagatcatca ctgtggagaa ccccaagatg ccttatgaga tgaaggagat ggcactggag
     2761 gccattgtgc agctctggcg catccccagc tttgtcacag agctctacat caactatgat
     2821 tgtgactact actgttccaa cctctttgag gaactcacaa agctgctgtc caagaatgcc
     2881 ttccctgtgt ctggtcaact ctatacaaca cacctactat ctcttgatgc cctattgaca
     2941 gtgattgaca gcaccgaggc ccactgccag gctaaagtcc tcaacagcct cacccagcaa
     3001 gagaagaagg agacagccag accaagctgt gagatagtag atggcacccg agaagctagc
     3061 aatactgaga gaactgccag cgatgggaaa gctgtaggca tggcctcaga catcccaggc
     3121 ctgcatctgc caggtggagg gcggctgcca ccagaacatg ggaaatcagg atgcagtgat
     3181 ctggaggaag ctgttgactc tggggctgac aaaaagtttg cccggaagcc accccgattt
     3241 tcctgtctcc tgccagatcc acgggaacta attgaaatta aaaacaaaaa gaagctgcta
     3301 atcactggca cagagcagtt caatcagaaa ccaaagaagg ggattcagtt tctgcaagag
     3361 aaaggcctcc tcaccatccc aatggacaac acagaggttg ctcagtggct ccgagagaac
     3421 cctcggctgg acaagaagat gattggagag tttgtgagtg accgcaaaaa cattgacctg
     3481 ttggagagct ttgtgagcac cttcagtttt cagggtctgc gactggacga agccctccgc
     3541 ctctacctgg aagccttccg tttgcctggg gaagcaccag tcatccagag gttgctggag
     3601 gcattcacag agcgttggat gaattgtaat ggctccccat ttgccaatag cgatgcctgc
     3661 ttttccctgg cctatgctgt catcatgctt aatactgacc agcacaacca caatgttcgt
     3721 aaacagaatg cacccatgac cctggaggag tttcgcaaaa atctgaaagg tgtgaatgga
     3781 ggcaaggact ttgagcaaga catcctggag gacatgtacc atgccatcaa gaatgaggaa
     3841 attgtaatgc ctgaggagca gacaggcttg gttcgggaga actatgtgtg gaatgtgctg
     3901 cttcatcgag gtgccacccc tgagggcata ttcctgcgtg tgcctactgc cagctatgat
     3961 cttgacctct tcaccatgac ctggggcccc actattgctg ctctctctta tgtctttgac
     4021 aaaagccttg aggagacaat catccagaaa gccatctcag gcttcaggaa gtgcgccatg
     4081 atctccgccc actatggcct cagcgatgtg tttgacaatc tcatcatctc tctatgcaaa
     4141 ttcacagctc tcagcagtga gtctattgag aacctgccca gtgtatttgg aagcaaccct
     4201 aaagcccata ttgcagccaa gacagtattc catttggccc atcgtcatgg tgacatcctg
     4261 cgggagggct ggaagaatat catggaggcc atgctgcagc tcttccgagc ccaactactg
     4321 cccaaggcta tgatagaggt agaagatttc gtggatccca atggcaagat ctctctacag
     4381 cgggaagaga caccatcaaa ccgaggagag tcaacagtgc tgagctttgt gagctggcta
     4441 acactgagtg gtcctgagca gtctagtgtt cggggcccat ccactgaaaa ccaagaggcc
     4501 aagagagtgg ccttagagtg tataaagcaa tgtgacccag aaaaaatgat cacagaaagc
     4561 aagttcctcc agctggagtc actacaggag ctcatgaagg ctctggtctc agtgacacca
     4621 gatgaagaga catatgatga ggaagatgct gctttctgcc tagagatgct gctaaggatt
     4681 gtgttggaga acagggatcg tgtgggctgt gtgtggcaga ctgttcgaga ccatctatac
     4741 cacctctgtg ttcaggcaca agatttctgc ttccttgtgg agcgggcagt ggtggggttg
     4801 ctacgcctgg ccattcggct tctccggaga gaagagatca gtgctcaggt gctgctctcc
     4861 ctgcgcattt tgctactgat gaagcccagt gtgctatccc gagtcagcca ccaggttgcg
     4921 tatgggctcc atgaactcct gaagaccaat gcagccaaca tccactcagg tgatgactgg
     4981 gccacactct tcacactgct ggagtgcatc ggctcaggtg tgaagcctcc agctgctctg
     5041 caggccacag ccagggcaga tgcacctgat gccggggccc agtcagatag tgagctccca
     5101 tcctaccatc agaatgacgt gagcctggat cgagggtaca cttccgactc agaggtctac
     5161 actgaccatg gcaggccggg caagatacac cgatcagcca cagatgccga tgtggtcaac
     5221 agtggttggt tagtggttgg gaaggatgac gttgataact ccaagccagg gcccagccgc
     5281 ccaggccctt cacccctgat caatcaatac agcctaacag tgggactgga tttggggcca
     5341 cacgacacta agtctctgct taagtgtgtg gaatcgctgt ccttcatcgt gcgtgatgct
     5401 gcccacatca cacctgacaa ctttgagctc tgcgtcaaga ctctccggat ctttgtggag
     5461 gccagtctga atggcggatg caagtcccag gagaaacgtg gcaagagtca caaatatgac
     5521 agcaaaggga accgcttcaa gaagaaatcc aaagagggat caatgcttcg ccggcctcga
     5581 acctccagcc aacatgcctc tcggggcggg cagagtgatg atgatgagga cgaaggcgtg
     5641 cctgccagct accatacggt gtctttacag gtcagtcagg acttgctaga cctgatgcac
     5701 accctgcaca cgcgggcagc ctctatctac agctcatggg cggaggagca acgccacctg
     5761 gagacaggtg gccagaagat tgaagctgat tctcgcaccc tctgggccca ctgctggtgc
     5821 cctttactgc agggtattgc ctgcctgtgc tgcgatgccc ggcgccaggt acggatgcag
     5881 gcactgacct atctgcagcg agcactactt gtacatgatc tgcaaaagct agatgccctg
     5941 gaatgggagt cctgttttaa caaggtgctg tttcctctac ttaccaagct cttggagaac
     6001 atcagccctg cagatgtggg tgggatggag gagacccgga tgagggcttc cacattgctc
     6061 tctaaggtct tcctgcagca cctgtctcca ctgctgtcac tctctacctt tgcggccctc
     6121 tggctcacca tcttggactt catggacaag tacatgcacg caggctccag cgacttactg
     6181 tcagaggcga tccctgagtc tctgaagaac atgcttctgg tgatggacac agcggagatt
     6241 ttccacagtg cagatgcacg gggaggcggc ccctcggccc tctgggagat cacctgggaa
     6301 cgcattgact gttttctccc tcacctacga gatgaactct tcaagcagac cgtcatccag
     6361 gaccccatgc ccatggagcc tcaaggccaa aagcctctcg cctcagccca cctgacttcc
     6421 gctgctggcg acactaggac acctggccat ccaccgcccc cagagattcc atctgagctg
     6481 ggggcctgtg actttgagaa gcccgagagc ccccgagccg ccagcagcag ctccccagga
     6541 tcaccagtgg cctcaagccc cagcaggctg agccccaccc ccgacgggcc tccacccttg
     6601 gctcagcccc cactgatcct gcagcccttg gcctccccac tgcaggtggg cgtgccacct
     6661 atgactctgc ccatcatcct caaccctgcg ctcatcgagg ccacctcacc agtgcccctc
     6721 ctggccacac cccgccccac agatcccata cccacctctg aggtcaacta aggcaggtca
     6781 ctcagagatc aggaccagtg cttcccacca ggctttcctt gaccccactt ctggctgtcc
     6841 tgcgggccac aagctcttca ggccaagtca gagctgctgt tgctgccact tggatgggga
     6901 cctgaaaaag agaatgttga tagccccagc taagaccccc aatcagctgt gggacctttt
     6961 tcctcctctg cgctccattc ctgggggttc agcctgagag tgaactcagc tgtcatctgc
     7021 agcctctgcc tccagcccgg cagctctggg gaggcatccg tgtgccggcc ctgcagtgcc
     7081 tgcccacggt caggcattga aaactaagcc caaccactct gcactttgtt tcccactccc
     7141 attagccctg ggccacctcc tccagttctt cctcttttac taattagttg gtcagtttgg
     7201 agagttgact ggcaccatgg agggtaggca ggtgggggct gggtggggga ctgcccacag
     7261 gagcatgtac atatggaaaa acagaacaac agtggacttt ttatgatata ataaatgtct
     7321 tagtaccaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaagcggc cgctcgagtc
     7381 tagagggccc gtttaaaccc gctgatcagc ctcgactgtg ccttctagtt gccagccatc
     7441 tgttgtttgc ccctcccccg tgccttcctt gaccctggaa ggtgccactc ccactgtcct
     7501 ttcctaataa aatgaggaaa ttgcatcgca ttgtctgagt aggtgtcatt ctattctggg
     7561 gggtggggtg gggcaggaca gcaaggggga ggattgggaa gacaatagca ggcatgctgg
     7621 ggatgcggtg ggctctatgg cttctgaggc ggaaagaacc agctggggct ctagggggta
     7681 tccccacgcg ccctgtagcg gcgcattaag cgcggcgggt gtggtggtta cgcgcagcgt
     7741 gaccgctaca cttgccagcg ccctagcgcc cgctcctttc gctttcttcc cttcctttct
     7801 cgccacgttc gccggctttc cccgtcaagc tctaaatcgg gggctccctt tagggttccg
     7861 atttagtgct ttacggcacc tcgaccccaa aaaacttgat tagggtgatg gttcacgtag
     7921 tgggccatcg ccctgataga cggtttttcg ccctttgacg ttggagtcca cgttctttaa
     7981 tagtggactc ttgttccaaa ctggaacaac actcaaccct atctcggtct attcttttga
     8041 tttataaggg attttgccga tttcggccta ttggttaaaa aatgagctga tttaacaaaa
     8101 atttaacgcg aattaattct gtggaatgtg tgtcagttag ggtgtggaaa gtccccaggc
     8161 tccccagcag gcagaagtat gcaaagcatg catctcaatt agtcagcaac caggtgtgga
     8221 aagtccccag gctccccagc aggcagaagt atgcaaagca tgcatctcaa ttagtcagca
     8281 accatagtcc cgcccctaac tccgcccatc ccgcccctaa ctccgcccag ttccgcccat
     8341 tctccgcccc atggctgact aatttttttt atttatgcag aggccgaggc cgcctctgcc
     8401 tctgagctat tccagaagta gtgaggaggc ttttttggag gcctaggctt ttgcaaaaag
     8461 ctcccgggag cttgtatatc cattttcgga tctgatcagc acgtgttgac aattaatcat
     8521 cggcatagta tatcggcata gtataatacg acaaggtgag gaactaaacc atggccaagt
     8581 tgaccagtgc cgttccggtg ctcaccgcgc gcgacgtcgc cggagcggtc gagttctgga
     8641 ccgaccggct cgggttctcc cgggacttcg tggaggacga cttcgccggt gtggtccggg
     8701 acgacgtgac cctgttcatc agcgcggtcc aggaccaggt ggtgccggac aacaccctgg
     8761 cctgggtgtg ggtgcgcggc ctggacgagc tgtacgccga gtggtcggag gtcgtgtcca
     8821 cgaacttccg ggacgcctcc gggccggcca tgaccgagat cggcgagcag ccgtgggggc
     8881 gggagttcgc cctgcgcgac ccggccggca actgcgtgca cttcgtggcc gaggagcagg
     8941 actgacacgt gctacgagat ttcgattcca ccgccgcctt ctatgaaagg ttgggcttcg
     9001 gaatcgtttt ccgggacgcc ggctggatga tcctccagcg cggggatctc atgctggagt
     9061 tcttcgccca ccccaacttg tttattgcag cttataatgg ttacaaataa agcaatagca
     9121 tcacaaattt cacaaataaa gcattttttt cactgcattc tagttgtggt ttgtccaaac
     9181 tcatcaatgt atcttatcat gtctgtatac cgtcgacctc tagctagagc ttggcgtaat
     9241 catggtcata gctgtttcct gtgtgaaatt gttatccgct cacaattcca cacaacatac
     9301 gagccggaag cataaagtgt aaagcctggg gtgcctaatg agtgagctaa ctcacattaa
     9361 ttgcgttgcg ctcactgccc gctttccagt cgggaaacct gtcgtgccag ctgcattaat
     9421 gaatcggcca acgcgcgggg agaggcggtt tgcgtattgg gcgctcttcc gcttcctcgc
     9481 tcactgactc gctgcgctcg gtcgttcggc tgcggcgagc ggtatcagct cactcaaagg
     9541 cggtaatacg gttatccaca gaatcagggg ataacgcagg aaagaacatg tgagcaaaag
     9601 gccagcaaaa ggccaggaac cgtaaaaagg ccgcgttgct ggcgtttttc cataggctcc
     9661 gcccccctga cgagcatcac aaaaatcgac gctcaagtca gaggtggcga aacccgacag
     9721 gactataaag ataccaggcg tttccccctg gaagctccct cgtgcgctct cctgttccga
     9781 ccctgccgct taccggatac ctgtccgcct ttctcccttc gggaagcgtg gcgctttctc
     9841 atagctcacg ctgtaggtat ctcagttcgg tgtaggtcgt tcgctccaag ctgggctgtg
     9901 tgcacgaacc ccccgttcag cccgaccgct gcgccttatc cggtaactat cgtcttgagt
     9961 ccaacccggt aagacacgac ttatcgccac tggcagcagc cactggtaac aggattagca
    10021 gagcgaggta tgtaggcggt gctacagagt tcttgaagtg gtggcctaac tacggctaca
    10081 ctagaagaac agtatttggt atctgcgctc tgctgaagcc agttaccttc ggaaaaagag
    10141 ttggtagctc ttgatccggc aaacaaacca ccgctggtag cggtggtttt tttgtttgca
    10201 agcagcagat tacgcgcaga aaaaaaggat ctcaagaaga tcctttgatc ttttctacgg
    10261 ggtctgacgc tcagtggaac gaaaactcac gttaagggat tttggtcatg agattatcaa
    10321 aaaggatctt cacctagatc cttttaaatt aaaaatgaag ttttaaatca atctaaagta
    10381 tatatgagta aacttggtct gacagttacc aatgcttaat cagtgaggca cctatctcag
    10441 cgatctgtct atttcgttca tccatagttg cctgactccc cgtcgtgtag ataactacga
    10501 tacgggaggg cttaccatct ggccccagtg ctgcaatgat accgcgagac ccacgctcac
    10561 cggctccaga tttatcagca ataaaccagc cagccggaag ggccgagcgc agaagtggtc
    10621 ctgcaacttt atccgcctcc atccagtcta ttaattgttg ccgggaagct agagtaagta
    10681 gttcgccagt taatagtttg cgcaacgttg ttgccattgc tacaggcatc gtggtgtcac
    10741 gctcgtcgtt tggtatggct tcattcagct ccggttccca acgatcaagg cgagttacat
    10801 gatcccccat gttgtgcaaa aaagcggtta gctccttcgg tcctccgatc gttgtcagaa
    10861 gtaagttggc cgcagtgtta tcactcatgg ttatggcagc actgcataat tctcttactg
    10921 tcatgccatc cgtaagatgc ttttctgtga ctggtgagta ctcaaccaag tcattctgag
    10981 aatagtgtat gcggcgaccg agttgctctt gcccggcgtc aatacgggat aataccgcgc
    11041 cacatagcag aactttaaaa gtgctcatca ttggaaaacg ttcttcgggg cgaaaactct
    11101 caaggatctt accgctgttg agatccagtt cgatgtaacc cactcgtgca cccaactgat
    11161 cttcagcatc ttttactttc accagcgttt ctgggtgagc aaaaacagga aggcaaaatg
    11221 ccgcaaaaaa gggaataagg gcgacacgga aatgttgaat actcatactc ttcctttttc
    11281 aatattattg aagcatttat cagggttatt gtctcatgag cggatacata tttgaatgta
    11341 tttagaaaaa taaacaaata ggggttccgc gcacatttcc ccgaaaagtg ccacctga
//
