LOCUS       sequence-410951-       11550 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Mammalian expression of hGBF1(E794K).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11550)
  AUTHORS   Kawamoto K, Yoshida Y, Tamaki H, Torii S, Shinotsuka C, Yamashina S,
            Nakayama K
  TITLE     GBF1, a guanine nucleotide exchange factor for ADP-ribosylation 
            factors, is localized to the cis-Golgi and involved in membrane 
            association of the COPI coat.
  JOURNAL   Traffic. 2002 Jul;3(7):483-95. doi: 
            10.1034/j.1600-0854.2002.30705.x.
  PUBMED    12047556
REFERENCE   2  (bases 1 to 11550)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1034/j.1600-0854.2002.30705"; journalName: "Traffic"; date: 
            "2002-07"; volume: "3"; issue: "7"; pages: "483-95"
COMMENT     Sequence Label: pcDNA3-HA-GBF1(E794K)
FEATURES             Location/Qualifiers
     source          1..11550
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(60..79)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        251..630
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        631..834
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     785..805
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     879..898
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        879..897
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             917..943
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     promoter        complement(7119..7137)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(7120..7137)
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     complement(7157..7174)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    7163..7387
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      7433..7861
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(7520..7539)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     7730..7751
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(7870..7890)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        7875..8204
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      8055..8190
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     8117..8136
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     CDS             8271..9065
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(8325..8344)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     8935..8954
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     polyA_signal    9239..9360
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(9276..9295)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     9330..9349
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(9409..9425)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(9409..9425)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(9422..9444)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    9433..9449
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(9457..9463,9464..9481,9482..9487))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    9502..9523
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(9640..9657)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(9811..10399)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(9891..9910)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(10570..11361,11362..11430))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     11193..11212
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(11431..11535)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 agtgccacct gacgtcgacg gatcgggaga tctcccgatc ccctatggtg cactctcagt
       61 acaatctgct ctgatgccgc atagttaagc cagtatctgc tccctgcttg tgtgttggag
      121 gtcgctgagt agtgcgcgag caaaatttaa gctacaacaa ggcaaggctt gaccgacaat
      181 tgcatgaaga atctgcttag ggttaggcgt tttgcgctgc ttcgcgatgt acgggccaga
      241 tatacgcgtt gacattgatt attgactagt tattaatagt aatcaattac ggggtcatta
      301 gttcatagcc catatatgga gttccgcgtt acataactta cggtaaatgg cccgcctggc
      361 tgaccgccca acgacccccg cccattgacg tcaataatga cgtatgttcc catagtaacg
      421 ccaataggga ctttccattg acgtcaatgg gtggagtatt tacggtaaac tgcccacttg
      481 gcagtacatc aagtgtatca tatgccaagt acgcccccta ttgacgtcaa tgacggtaaa
      541 tggcccgcct ggcattatgc ccagtacatg accttatggg actttcctac ttggcagtac
      601 atctacgtat tagtcatcgc tattaccatg gtgatgcggt tttggcagta catcaatggg
      661 cgtggatagc ggtttgactc acggggattt ccaagtctcc accccattga cgtcaatggg
      721 agtttgtttt ggcaccaaaa tcaacgggac tttccaaaat gtcgtaacaa ctccgcccca
      781 ttgacgcaaa tgggcggtag gcgtgtacgg tgggaggtct atataagcag agctctctgg
      841 ctaactagag aacccactgc ttactggctt atcgaaatta atacgactca ctatagggag
      901 acccaagctt accatgtacc cgtacgacgt gcccgactac gcgggatcta ccatggtgga
      961 taagaatatt tacatcattc aaggggagat taacattgtg gttggggcca tcaaacgaaa
     1021 tgcccgatgg agcacccata caccactgga tgaagaacgg gatcctctgc tgcatagttt
     1081 cggtcatcta aaggaggttt taaacagtat aacagaactc tcagaaattg agcccaatgt
     1141 attccttcga ccttttctgg aagtgattcg ctctgaagat accactggcc ctatcactgg
     1201 actggcactc acctctgtca acaagttcct gtcctatgca ctcatagatc ccacccatga
     1261 gggcacagca gagggcatgg agaacatggc agatgctgtc acccatgctc gttttgtggg
     1321 cacggatcct gccagtgatg aagttgtcct gatgaaaatc cttcaggttc tacggactct
     1381 gctgctaacc ccagtgggtg cccacctaac caatgaatct gtgcgtgaga ttatgcagtc
     1441 ttgcttccgg atctgctttg aaatgaggct cagtgagtta ttgagaaaat ccgcagagca
     1501 cactctcgta gacatggtgc agctgctctt cacaaggtta cctcagttta aagaagaacc
     1561 caagaactat gtggggacca acatgaagaa gctgaaaatg agagccggag gcatgagtga
     1621 ttcatccaaa tggaagaaac agaagagatc ccctcggccc ccacgccata tgaccaaagt
     1681 cacaccaggt tcagagctgc ccactcccaa tggaaccacc ttatcatcta acctcactgg
     1741 tggcatgccc ttcattgatg tgcccactcc catctcctct gcaagttcag aagctgcctc
     1801 agcagtggtc agtccctcta cagacagtgg cctggaattc tcctcccaaa ccacttccaa
     1861 ggaagacctt actgatctag agcaacctgg ctctccaggg tacagcacag ctacagagcc
     1921 tggaagcagt gagctaggtg ttcccgagca gcctgacctc caggaaggga cccatgtgga
     1981 aaagtcccag tcagcatctg tggagtccat ccctgaagtg ttagaggagt gcacgtcccc
     2041 tgccgaccac tctgactctg cctctgtcca tgacatggat tacgtcaatc cccggggcgt
     2101 gcgctttaca cagtcctccc agaaagaagg cacagctttg gtcccctatg gtcttccctg
     2161 catccgcgag ctcttccgct tcctcatctc cctcaccaat ccacacgacc gccataactc
     2221 agaggttatg attcacatgg gactgcattt gctgacagtg gcccttgagt cagcccctgt
     2281 agcccagtgc cagaccctct tgggcctcat caaggatgag atgtgccgtc acttattcca
     2341 gctactcagc atagagcgac taaaccttta tgctgcttcc ctgcgagtat gcttcctact
     2401 gtttgagagc atgcgagagc acctcaagtt ccaaatggag atgtacatca aaaagcttat
     2461 ggagatcatc actgtggaga accccaagat gccttatgag atgaaggaga tggcactgga
     2521 ggccattgtg cagctctggc gcatccccag ctttgtcaca gagctctaca tcaactatga
     2581 ttgtgactac tactgttcca acctctttga ggaactcaca aagctgctgt ccaagaatgc
     2641 cttccctgtg tctggtcaac tctatacaac acacctacta tctcttgatg ccctattgac
     2701 agtgattgac agcaccgagg cccactgcca ggctaaagtc ctcaacagcc tcacccagca
     2761 agagaagaag gagacagcca gaccaagctg tgagatagta gatggcaccc gagaagctag
     2821 caatactgag agaactgcca gcgatgggaa agctgtaggc atggcctcag acatcccagg
     2881 cctgcatctg ccaggtggag ggcggctgcc accagaacat gggaaatcag gatgcagtga
     2941 tctggaggaa gctgttgact ctggggctga caaaaagttt gcccggaagc caccccgatt
     3001 ttcctgtctc ctgccagatc cacgggaact aattgaaatt aaaaacaaaa agaagctgct
     3061 aatcactggc acagagcagt tcaatcagaa accaaagaag gggattcagt ttctgcaaga
     3121 gaaaggcctc ctcaccatcc caatggacaa cacagaggtt gctcagtggc tccgagagaa
     3181 ccctcggctg gacaagaaga tgattggaga gtttgtgagt gaccgcaaaa acattgacct
     3241 gttggagagc tttgtgagca ccttcagttt tcagggtctg cgactggacg aagccctccg
     3301 cctctacctg gaagccttcc gtttgcccgg gaaagcacca gtcatccaga ggttgctgga
     3361 ggcattcaca gagcgttgga tgaattgtaa tggctcccca tttgccaata gcgatgcctg
     3421 cttttccctg gcctatgctg tcatcatgct taatactgac cagcacaacc acaatgttcg
     3481 taaacagaat gcacccatga ccctggagga gtttcgcaaa aatctgaaag gtgtgaatgg
     3541 aggcaaggac tttgagcaag acatcctgga ggacatgtac catgccatca agaatgagga
     3601 aattgtaatg cctgaggagc agacaggctt ggttcgggag aactatgtgt ggaatgtgct
     3661 gcttcatcga ggtgccaccc ctgagggcat attcctgcgt gtgcctactg ccagctatga
     3721 tcttgacctc ttcaccatga cctggggccc cactattgct gctctctctt atgtctttga
     3781 caaaagcctt gaggagacaa tcatccagaa agccatctca ggcttcagga agtgcgccat
     3841 gatctccgcc cactatggcc tcagcgatgt gtttgacaat ctcatcatct ctctatgcaa
     3901 attcacagct ctcagcagtg agtctattga gaacctgccc agtgtatttg gaagcaaccc
     3961 taaagcccat attgcagcca agacagtatt ccatttggcc catcgtcatg gtgacatcct
     4021 gcgggagggc tggaagaata tcatggaggc catgctgcag ctcttccgag cccaactact
     4081 gcccaaggct atgatagagg tagaagattt cgtggatccc aatggcaaga tctctctaca
     4141 gcgggaagag acaccatcaa accgaggaga gtcaacagtg ctgagctttg tgagctggct
     4201 aacactgagt ggtcctgagc agtctagtgt tcggggccca tccactgaaa accaagaggc
     4261 caagagagtg gccttagagt gtataaagca atgtgaccca gaaaaaatga tcacagaaag
     4321 caagttcctc cagctggagt cactacagga gctcatgaag gctctggtct cagtgacacc
     4381 agatgaagag acatatgatg aggaagatgc tgctttctgc ctagagatgc tgctaaggat
     4441 tgtgttggag aacagggatc gtgtgggctg tgtgtggcag actgttcgag accatctata
     4501 ccacctctgt gttcaggcac aagatttctg cttccttgtg gagcgggcag tggtggggtt
     4561 gctacgcctg gccattcggc ttctccggag agaagagatc agtgctcagg tgctgctctc
     4621 cctgcgcatt ttgctactga tgaagcccag tgtgctatcc cgagtcagcc accaggttgc
     4681 gtatgggctc catgaactcc tgaagaccaa tgcagccaac atccactcag gtgatgactg
     4741 ggccacactc ttcacactgc tggagtgcat cggctcaggt gtgaagcctc cagctgctct
     4801 gcaggccaca gccagggcag atgcacctga tgccggggcc cagtcagata gtgagctccc
     4861 atcctaccat cagaatgacg tgagcctgga tcgagggtac acttccgact cagaggtcta
     4921 cactgaccat ggcaggccgg gcaagataca ccgatcagcc acagatgccg atgtggtcaa
     4981 cagtggttgg ttagtggttg ggaaggatga cgttgataac tccaagccag ggcccagccg
     5041 cccaggccct tcacccctga tcaatcaata cagcctaaca gtgggactgg atttggggcc
     5101 acacgacact aagtctctgc ttaagtgtgt ggaatcgctg tccttcatcg tgcgtgatgc
     5161 tgcccacatc acacctgaca actttgagct ctgcgtcaag actctccgga tctttgtgga
     5221 ggccagtctg aatggcggat gcaagtccca ggagaaacgt ggcaagagtc acaaatatga
     5281 cagcaaaggg aaccgcttca agaagaaatc caaagaggga tcaatgcttc gccggcctcg
     5341 aacctccagc caacatgcct ctcggggcgg gcagagtgat gatgatgagg acgaaggcgt
     5401 gcctgccagc taccatacgg tgtctttaca ggtcagtcag gacttgctag acctgatgca
     5461 caccctgcac acgcgggcag cctctatcta cagctcatgg gcggaggagc aacgccacct
     5521 ggagacaggt ggccagaaga ttgaagctga ttctcgcacc ctctgggccc actgctggtg
     5581 ccctttactg cagggtattg cctgcctgtg ctgcgatgcc cggcgccagg tacggatgca
     5641 ggcactgacc tatctgcagc gagcactact tgtacatgat ctgcaaaagc tagatgccct
     5701 ggaatgggag tcctgtttta acaaggtgct gtttcctcta cttaccaagc tcttggagaa
     5761 catcagccct gcagatgtgg gtgggatgga ggagacccgg atgagggctt ccacattgct
     5821 ctctaaggtc ttcctgcagc acctgtctcc actgctgtca ctctctacct ttgcggccct
     5881 ctggctcacc atcttggact tcatggacaa gtacatgcac gcaggctcca gcgacttact
     5941 gtcagaggcg atccctgagt ctctgaagaa catgcttctg gtgatggaca cagcggagat
     6001 tttccacagt gcagatgcac ggggaggcgg cccctcggcc ctctgggaga tcacctggga
     6061 acgcattgac tgttttctcc ctcacctacg agatgaactc ttcaagcaga ccgtcatcca
     6121 ggaccccatg cccatggagc ctcaaggcca aaagcctctc gcctcagccc acctgacttc
     6181 cgctgctggc gacactagga cacctggcca tccaccgccc ccagagattc catctgagct
     6241 gggggcctgt gactttgaga agcccgagag cccccgagcc gccagcagca gctccccagg
     6301 atcaccagtg gcctcaagcc ccagcaggct gagccccacc cccgacgggc ctccaccctt
     6361 ggctcagccc ccactgatcc tgcagccctt ggcctcccca ctgcaggtgg gcgtgccacc
     6421 tatgactctg cccatcatcc tcaaccctgc gctcatcgag gccacctcac cagtgcccct
     6481 cctggccaca ccccgcccca cagatcccat acccacctct gaggtcaact aaggcaggtc
     6541 actcagagat caggaccagt gcttcccacc aggctttcct tgaccccact tctggctgtc
     6601 ctgcgggcca caagctcttc aggccaagtc agagctgctg ttgctgccac ttggatgggg
     6661 acctgaaaaa gagaatgttg atagccccag ctaagacccc caatcagctg tgggaccttt
     6721 ttcctcctct gcgctccatt cctgggggtt cagcctgaga gtgaactcag ctgtcatctg
     6781 cagcctctgc ctccagcccg gcagctctgg ggaggcatcc gtgtgccggc cctgcagtgc
     6841 ctgcccacgg tcaggcattg aaaactaagc ccaaccactc tgcactttgt ttcccactcc
     6901 cattagccct gggccacctc ctccagttct tcctctttta ctaattagtt ggtcagtttg
     6961 gagagttgac tggcaccatg gagggtaggc aggtgggggc tgggtggggg actgcccaca
     7021 ggagcatgta catatggaaa aacagaacaa cagtggactt tttatgatat aataaatgtc
     7081 ttagtaccag gctcgagcat gcatctagag ggccctattc tatagtgtca cctaaatgct
     7141 agagctcgct gatcagcctc gactgtgcct tctagttgcc agccatctgt tgtttgcccc
     7201 tcccccgtgc cttccttgac cctggaaggt gccactccca ctgtcctttc ctaataaaat
     7261 gaggaaattg catcgcattg tctgagtagg tgtcattcta ttctgggggg tggggtgggg
     7321 caggacagca agggggagga ttgggaagac aatagcaggc atgctgggga tgcggtgggc
     7381 tctatggctt ctgaggcgga aagaaccagc tggggctcta gggggtatcc ccacgcgccc
     7441 tgtagcggcg cattaagcgc ggcgggtgtg gtggttacgc gcagcgtgac cgctacactt
     7501 gccagcgccc tagcgcccgc tcctttcgct ttcttccctt cctttctcgc cacgttcgcc
     7561 ggctttcccc gtcaagctct aaatcggggg ctccctttag ggttccgatt tagtgcttta
     7621 cggcacctcg accccaaaaa acttgattag ggtgatggtt cacgtagtgg gccatcgccc
     7681 tgatagacgg tttttcgccc tttgacgttg gagtccacgt tctttaatag tggactcttg
     7741 ttccaaactg gaacaacact caaccctatc tcggtctatt cttttgattt ataagggatt
     7801 ttgccgattt cggcctattg gttaaaaaat gagctgattt aacaaaaatt taacgcgaat
     7861 taattctgtg gaatgtgtgt cagttagggt gtggaaagtc cccaggctcc ccagcaggca
     7921 gaagtatgca aagcatgcat ctcaattagt cagcaaccag gtgtggaaag tccccaggct
     7981 ccccagcagg cagaagtatg caaagcatgc atctcaatta gtcagcaacc atagtcccgc
     8041 ccctaactcc gcccatcccg cccctaactc cgcccagttc cgcccattct ccgccccatg
     8101 gctgactaat tttttttatt tatgcagagg ccgaggccgc ctctgcctct gagctattcc
     8161 agaagtagtg aggaggcttt tttggaggcc taggcttttg caaaaagctc ccgggagctt
     8221 gtatatccat tttcggatct gatcaagaga caggatgagg atcgtttcgc atgattgaac
     8281 aagatggatt gcacgcaggt tctccggccg cttgggtgga gaggctattc ggctatgact
     8341 gggcacaaca gacaatcggc tgctctgatg ccgccgtgtt ccggctgtca gcgcaggggc
     8401 gcccggttct ttttgtcaag accgacctgt ccggtgccct gaatgaactg caggacgagg
     8461 cagcgcggct atcgtggctg gccacgacgg gcgttccttg cgcagctgtg ctcgacgttg
     8521 tcactgaagc gggaagggac tggctgctat tgggcgaagt gccggggcag gatctcctgt
     8581 catctcacct tgctcctgcc gagaaagtat ccatcatggc tgatgcaatg cggcggctgc
     8641 atacgcttga tccggctacc tgcccattcg accaccaagc gaaacatcgc atcgagcgag
     8701 cacgtactcg gatggaagcc ggtcttgtcg atcaggatga tctggacgaa gagcatcagg
     8761 ggctcgcgcc agccgaactg ttcgccaggc tcaaggcgcg catgcccgac ggcgaggatc
     8821 tcgtcgtgac ccatggcgat gcctgcttgc cgaatatcat ggtggaaaat ggccgctttt
     8881 ctggattcat cgactgtggc cggctgggtg tggcggaccg ctatcaggac atagcgttgg
     8941 ctacccgtga tattgctgaa gagcttggcg gcgaatgggc tgaccgcttc ctcgtgcttt
     9001 acggtatcgc cgctcccgat tcgcagcgca tcgccttcta tcgccttctt gacgagttct
     9061 tctgagcggg actctggggt tcgaaatgac cgaccaagcg acgcccaacc tgccatcacg
     9121 agatttcgat tccaccgccg ccttctatga aaggttgggc ttcggaatcg ttttccggga
     9181 cgccggctgg atgatcctcc agcgcgggga tctcatgctg gagttcttcg cccaccccaa
     9241 cttgtttatt gcagcttata atggttacaa ataaagcaat agcatcacaa atttcacaaa
     9301 taaagcattt ttttcactgc attctagttg tggtttgtcc aaactcatca atgtatctta
     9361 tcatgtctgt ataccgtcga cctctagcta gagcttggcg taatcatggt catagctgtt
     9421 tcctgtgtga aattgttatc cgctcacaat tccacacaac atacgagccg gaagcataaa
     9481 gtgtaaagcc tggggtgcct aatgagtgag ctaactcaca ttaattgcgt tgcgctcact
     9541 gcccgctttc cagtcgggaa acctgtcgtg ccagctgcat taatgaatcg gccaacgcgc
     9601 ggggagaggc ggtttgcgta ttgggcgctc ttccgcttcc tcgctcactg actcgctgcg
     9661 ctcggtcgtt cggctgcggc gagcggtatc agctcactca aaggcggtaa tacggttatc
     9721 cacagaatca ggggataacg caggaaagaa catgtgagca aaaggccagc aaaaggccag
     9781 gaaccgtaaa aaggccgcgt tgctggcgtt tttccatagg ctccgccccc ctgacgagca
     9841 tcacaaaaat cgacgctcaa gtcagaggtg gcgaaacccg acaggactat aaagatacca
     9901 ggcgtttccc cctggaagct ccctcgtgcg ctctcctgtt ccgaccctgc cgcttaccgg
     9961 atacctgtcc gcctttctcc cttcgggaag cgtggcgctt tctcatagct cacgctgtag
    10021 gtatctcagt tcggtgtagg tcgttcgctc caagctgggc tgtgtgcacg aaccccccgt
    10081 tcagcccgac cgctgcgcct tatccggtaa ctatcgtctt gagtccaacc cggtaagaca
    10141 cgacttatcg ccactggcag cagccactgg taacaggatt agcagagcga ggtatgtagg
    10201 cggtgctaca gagttcttga agtggtggcc taactacggc tacactagaa gaacagtatt
    10261 tggtatctgc gctctgctga agccagttac cttcggaaaa agagttggta gctcttgatc
    10321 cggcaaacaa accaccgctg gtagcggtgg tttttttgtt tgcaagcagc agattacgcg
    10381 cagaaaaaaa ggatctcaag aagatccttt gatcttttct acggggtctg acgctcagtg
    10441 gaacgaaaac tcacgttaag ggattttggt catgagatta tcaaaaagga tcttcaccta
    10501 gatcctttta aattaaaaat gaagttttaa atcaatctaa agtatatatg agtaaacttg
    10561 gtctgacagt taccaatgct taatcagtga ggcacctatc tcagcgatct gtctatttcg
    10621 ttcatccata gttgcctgac tccccgtcgt gtagataact acgatacggg agggcttacc
    10681 atctggcccc agtgctgcaa tgataccgcg agacccacgc tcaccggctc cagatttatc
    10741 agcaataaac cagccagccg gaagggccga gcgcagaagt ggtcctgcaa ctttatccgc
    10801 ctccatccag tctattaatt gttgccggga agctagagta agtagttcgc cagttaatag
    10861 tttgcgcaac gttgttgcca ttgctacagg catcgtggtg tcacgctcgt cgtttggtat
    10921 ggcttcattc agctccggtt cccaacgatc aaggcgagtt acatgatccc ccatgttgtg
    10981 caaaaaagcg gttagctcct tcggtcctcc gatcgttgtc agaagtaagt tggccgcagt
    11041 gttatcactc atggttatgg cagcactgca taattctctt actgtcatgc catccgtaag
    11101 atgcttttct gtgactggtg agtactcaac caagtcattc tgagaatagt gtatgcggcg
    11161 accgagttgc tcttgcccgg cgtcaatacg ggataatacc gcgccacata gcagaacttt
    11221 aaaagtgctc atcattggaa aacgttcttc ggggcgaaaa ctctcaagga tcttaccgct
    11281 gttgagatcc agttcgatgt aacccactcg tgcacccaac tgatcttcag catcttttac
    11341 tttcaccagc gtttctgggt gagcaaaaac aggaaggcaa aatgccgcaa aaaagggaat
    11401 aagggcgaca cggaaatgtt gaatactcat actcttcctt tttcaatatt attgaagcat
    11461 ttatcagggt tattgtctca tgagcggata catatttgaa tgtatttaga aaaataaaca
    11521 aataggggtt ccgcgcacat ttccccgaaa
//
