LOCUS       sequence-410957-       11549 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Mammalian expression of hGBF1.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11549)
  AUTHORS   Kawamoto K, Yoshida Y, Tamaki H, Torii S, Shinotsuka C, Yamashina S,
            Nakayama K
  TITLE     GBF1, a guanine nucleotide exchange factor for ADP-ribosylation 
            factors, is localized to the cis-Golgi and involved in membrane 
            association of the COPI coat.
  JOURNAL   Traffic. 2002 Jul;3(7):483-95. doi: 
            10.1034/j.1600-0854.2002.30705.x.
  PUBMED    12047556
REFERENCE   2  (bases 1 to 11549)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1034/j.1600-0854.2002.30705"; journalName: "Traffic"; date: 
            "2002-07"; volume: "3"; issue: "7"; pages: "483-95"
COMMENT     Sequence Label: pcDNA3-HA-GBF1
FEATURES             Location/Qualifiers
     source          1..11549
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(67..86)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        258..637
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        638..841
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     792..812
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     886..905
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        886..904
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             924..950
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     promoter        complement(7125..7143)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(7126..7143)
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     complement(7163..7180)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    7169..7393
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      7439..7867
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(7526..7545)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     7736..7757
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(7876..7896)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        7881..8210
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      8061..8196
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     8123..8142
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     CDS             8277..9071
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(8331..8350)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     8941..8960
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     polyA_signal    9245..9366
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(9282..9301)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     9336..9355
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(9415..9431)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(9415..9431)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(9428..9450)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    9439..9455
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(9463..9469,9470..9487,9488..9493))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    9508..9529
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(9646..9663)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(9817..10405)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(9897..9916)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(10576..11367,11368..11436))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     11199..11218
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(11437..11541)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 cccgaaaagt gccacctgac gtcgacggat cgggagatct cccgatcccc tatggtgcac
       61 tctcagtaca atctgctctg atgccgcata gttaagccag tatctgctcc ctgcttgtgt
      121 gttggaggtc gctgagtagt gcgcgagcaa aatttaagct acaacaaggc aaggcttgac
      181 cgacaattgc atgaagaatc tgcttagggt taggcgtttt gcgctgcttc gcgatgtacg
      241 ggccagatat acgcgttgac attgattatt gactagttat taatagtaat caattacggg
      301 gtcattagtt catagcccat atatggagtt ccgcgttaca taacttacgg taaatggccc
      361 gcctggctga ccgcccaacg acccccgccc attgacgtca ataatgacgt atgttcccat
      421 agtaacgcca atagggactt tccattgacg tcaatgggtg gagtatttac ggtaaactgc
      481 ccacttggca gtacatcaag tgtatcatat gccaagtacg ccccctattg acgtcaatga
      541 cggtaaatgg cccgcctggc attatgccca gtacatgacc ttatgggact ttcctacttg
      601 gcagtacatc tacgtattag tcatcgctat taccatggtg atgcggtttt ggcagtacat
      661 caatgggcgt ggatagcggt ttgactcacg gggatttcca agtctccacc ccattgacgt
      721 caatgggagt ttgttttggc accaaaatca acgggacttt ccaaaatgtc gtaacaactc
      781 cgccccattg acgcaaatgg gcggtaggcg tgtacggtgg gaggtctata taagcagagc
      841 tctctggcta actagagaac ccactgctta ctggcttatc gaaattaata cgactcacta
      901 tagggagacc caagcttacc atgtacccgt acgacgtgcc cgactacgcg ggatctacca
      961 tggtggataa gaatatttac atcattcaag gggagattaa cattgtggtt ggggccatca
     1021 aacgaaatgc ccgatggagc acccatacac cactggatga agaacgggat cctctgctgc
     1081 atagtttcgg tcatctaaag gaggttttaa acagtataac agaactctca gaaattgagc
     1141 ccaatgtatt ccttcgacct tttctggaag tgattcgctc tgaagatacc actggcccta
     1201 tcactggact ggcactcacc tctgtcaaca agttcctgtc ctatgcactc atagatccca
     1261 cccatgaggg cacagcagag ggcatggaga acatggcaga tgctgtcacc catgctcgtt
     1321 ttgtgggcac ggatcctgcc agtgatgaag ttgtcctgat gaaaatcctt caggttctac
     1381 ggactctgct gctaacccca gtgggtgccc acctaaccaa tgaatctgtg cgtgagatta
     1441 tgcagtcttg cttccggatc tgctttgaaa tgaggctcag tgagttattg agaaaatccg
     1501 cagagcacac tctcgtagac atggtgcagc tgctcttcac aaggttacct cagtttaaag
     1561 aagaacccaa gaactatgtg gggaccaaca tgaagaagct gaaaatgaga gccggaggca
     1621 tgagtgattc atccaaatgg aagaaacaga agagatcccc tcggccccca cgccatatga
     1681 ccaaagtcac accaggttca gagctgccca ctcccaatgg aaccacctta tcatctaacc
     1741 tcactggtgg catgcccttc attgatgtgc ccactcccat ctcctctgca agttcagaag
     1801 ctgcctcagc agtggtcagt ccctctacag acagtggcct ggaattctcc tcccaaacca
     1861 cttccaagga agaccttact gatctagagc aacctggctc tccagggtac agcacagcta
     1921 cagagcctgg aagcagtgag ctaggtgttc ccgagcagcc tgacctccag gaagggaccc
     1981 atgtggaaaa gtcccagtca gcatctgtgg agtccatccc tgaagtgtta gaggagtgca
     2041 cgtcccctgc cgaccactct gactctgcct ctgtccatga catggattac gtcaatcccc
     2101 ggggcgtgcg ctttacacag tcctcccaga aagaaggcac agctttggtc ccctatggtc
     2161 ttccctgcat ccgcgagctc ttccgcttcc tcatctccct caccaatcca cacgaccgcc
     2221 ataactcaga ggttatgatt cacatgggac tgcatttgct gacagtggcc cttgagtcag
     2281 cccctgtagc ccagtgccag accctcttgg gcctcatcaa ggatgagatg tgccgtcact
     2341 tattccagct actcagcata gagcgactaa acctttatgc tgcttccctg cgagtatgct
     2401 tcctactgtt tgagagcatg cgagagcacc tcaagttcca aatggagatg tacatcaaaa
     2461 agcttatgga gatcatcact gtggagaacc ccaagatgcc ttatgagatg aaggagatgg
     2521 cactggaggc cattgtgcag ctctggcgca tccccagctt tgtcacagag ctctacatca
     2581 actatgattg tgactactac tgttccaacc tctttgagga actcacaaag ctgctgtcca
     2641 agaatgcctt ccctgtgtct ggtcaactct atacaacaca cctactatct cttgatgccc
     2701 tattgacagt gattgacagc accgaggccc actgccaggc taaagtcctc aacagcctca
     2761 cccagcaaga gaagaaggag acagccagac caagctgtga gatagtagat ggcacccgag
     2821 aagctagcaa tactgagaga actgccagcg atgggaaagc tgtaggcatg gcctcagaca
     2881 tcccaggcct gcatctgcca ggtggagggc ggctgccacc agaacatggg aaatcaggat
     2941 gcagtgatct ggaggaagct gttgactctg gggctgacaa aaagtttgcc cggaagccac
     3001 cccgattttc ctgtctcctg ccagatccac gggaactaat tgaaattaaa aacaaaaaga
     3061 agctgctaat cactggcaca gagcagttca atcagaaacc aaagaagggg attcagtttc
     3121 tgcaagagaa aggcctcctc accatcccaa tggacaacac agaggttgct cagtggctcc
     3181 gagagaaccc tcggctggac aagaagatga ttggagagtt tgtgagtgac cgcaaaaaca
     3241 ttgacctgtt ggagagcttt gtgagcacct tcagttttca gggtctgcga ctggacgaag
     3301 ccctccgcct ctacctggaa gccttccgtt tgcctgggga agcaccagtc atccagaggt
     3361 tgctggaggc attcacagag cgttggatga attgtaatgg ctccccattt gccaatagcg
     3421 atgcctgctt ttccctggcc tatgctgtca tcatgcttaa tactgaccag cacaaccaca
     3481 atgttcgtaa acagaatgca cccatgaccc tggaggagtt tcgcaaaaat ctgaaaggtg
     3541 tgaatggagg caaggacttt gagcaagaca tcctggagga catgtaccat gccatcaaga
     3601 atgaggaaat tgtaatgcct gaggagcaga caggcttggt tcgggagaac tatgtgtgga
     3661 atgtgctgct tcatcgaggt gccacccctg agggcatatt cctgcgtgtg cctactgcca
     3721 gctatgatct tgacctcttc accatgacct ggggccccac tattgctgct ctctcttatg
     3781 tctttgacaa aagccttgag gagacaatca tccagaaagc catctcaggc ttcaggaagt
     3841 gcgccatgat ctccgcccac tatggcctca gcgatgtgtt tgacaatctc atcatctctc
     3901 tatgcaaatt cacagctctc agcagtgagt ctattgagaa cctgcccagt gtatttggaa
     3961 gcaaccctaa agcccatatt gcagccaaga cagtattcca tttggcccat cgtcatggtg
     4021 acatcctgcg ggagggctgg aagaatatca tggaggccat gctgcagctc ttccgagccc
     4081 aactactgcc caaggctatg atagaggtag aagatttcgt ggatcccaat ggcaagatct
     4141 ctctacagcg ggaagagaca ccatcaaacc gaggagagtc aacagtgctg agctttgtga
     4201 gctggctaac actgagtggt cctgagcagt ctagtgttcg gggcccatcc actgaaaacc
     4261 aagaggccaa gagagtggcc ttagagtgta taaagcaatg tgacccagaa aaaatgatca
     4321 cagaaagcaa gttcctccag ctggagtcac tacaggagct catgaaggct ctggtctcag
     4381 tgacaccaga tgaagagaca tatgatgagg aagatgctgc tttctgccta gagatgctgc
     4441 taaggattgt gttggagaac agggatcgtg tgggctgtgt gtggcagact gttcgagacc
     4501 atctatacca cctctgtgtt caggcacaag atttctgctt ccttgtggag cgggcagtgg
     4561 tggggttgct acgcctggcc attcggcttc tccggagaga agagatcagt gctcaggtgc
     4621 tgctctccct gcgcattttg ctactgatga agcccagtgt gctatcccga gtcagccacc
     4681 aggttgcgta tgggctccat gaactcctga agaccaatgc agccaacatc cactcaggtg
     4741 atgactgggc cacactcttc acactgctgg agtgcatcgg ctcaggtgtg aagcctccag
     4801 ctgctctgca ggccacagcc agggcagatg cacctgatgc cggggcccag tcagatagtg
     4861 agctcccatc ctaccatcag aatgacgtga gcctggatcg agggtacact tccgactcag
     4921 aggtctacac tgaccatggc aggccgggca agatacaccg atcagccaca gatgccgatg
     4981 tggtcaacag tggttggtta gtggttggga aggatgacgt tgataactcc aagccagggc
     5041 ccagccgccc aggcccttca cccctgatca atcaatacag cctaacagtg ggactggatt
     5101 tggggccaca cgacactaag tctctgctta agtgtgtgga atcgctgtcc ttcatcgtgc
     5161 gtgatgctgc ccacatcaca cctgacaact ttgagctctg cgtcaagact ctccggatct
     5221 ttgtggaggc cagtctgaat ggcggatgca agtcccagga gaaacgtggc aagagtcaca
     5281 aatatgacag caaagggaac cgcttcaaga agaaatccaa agagggatca atgcttcgcc
     5341 ggcctcgaac ctccagccaa catgcctctc ggggcgggca gagtgatgat gatgaggacg
     5401 aaggcgtgcc tgccagctac catacggtgt ctttacaggt cagtcaggac ttgctagacc
     5461 tgatgcacac cctgcacacg cgggcagcct ctatctacag ctcatgggcg gaggagcaac
     5521 gccacctgga gacaggtggc cagaagattg aagctgattc tcgcaccctc tgggcccact
     5581 gctggtgccc tttactgcag ggtattgcct gcctgtgctg cgatgcccgg cgccaggtac
     5641 ggatgcaggc actgacctat ctgcagcgag cactacttgt acatgatctg caaaagctag
     5701 atgccctgga atgggagtcc tgttttaaca aggtgctgtt tcctctactt accaagctct
     5761 tggagaacat cagccctgca gatgtgggtg ggatggagga gacccggatg agggcttcca
     5821 cattgctctc taaggtcttc ctgcagcacc tgtctccact gctgtcactc tctacctttg
     5881 cggccctctg gctcaccatc ttggacttca tggacaagta catgcacgca ggctccagcg
     5941 acttactgtc agaggcgatc cctgagtctc tgaagaacat gcttctggtg atggacacag
     6001 cggagatttt ccacagtgca gatgcacggg gaggcggccc ctcggccctc tgggagatca
     6061 cctgggaacg cattgactgt tttctccctc acctacgaga tgaactcttc aagcagaccg
     6121 tcatccagga ccccatgccc atggagcctc aaggccaaaa gcctctcgcc tcagcccacc
     6181 tgacttccgc tgctggcgac actaggacac ctggccatcc accgccccca gagattccat
     6241 ctgagctggg ggcctgtgac tttgagaagc ccgagagccc ccgagccgcc agcagcagct
     6301 ccccaggatc accagtggcc tcaagcccca gcaggctgag ccccaccccc gacgggcctc
     6361 cacccttggc tcagccccca ctgatcctgc agcccttggc ctccccactg caggtgggcg
     6421 tgccacctat gactctgccc atcatcctca accctgcgct catcgaggcc acctcaccag
     6481 tgcccctcct ggccacaccc cgccccacag atcccatacc cacctctgag gtcaactaag
     6541 gcaggtcact cagagatcag gaccagtgct tcccaccagg ctttccttga ccccacttct
     6601 ggctgtcctg cgggccacaa gctcttcagg ccaagtcaga gctgctgttg ctgccacttg
     6661 gatggggacc tgaaaaagag aatgttgata gccccagcta agacccccaa tcagctgtgg
     6721 gacctttttc ctcctctgcg ctccattcct gggggttcag cctgagagtg aactcagctg
     6781 tcatctgcag cctctgcctc cagcccggca gctctgggga ggcatccgtg tgccggccct
     6841 gcagtgcctg cccacggtca ggcattgaaa actaagccca accactctgc actttgtttc
     6901 ccactcccat tagccctggg ccacctcctc cagttcttcc tcttttacta attagttggt
     6961 cagtttggag agttgactgg caccatggag ggtaggcagg tgggggctgg gtgggggact
     7021 gcccacagga gcatgtacat atggaaaaac agaacaacag tggacttttt ataaaaaaaa
     7081 aaaaaaaaaa gcggccgctc gagcatgcat ctagagggcc ctattctata gtgtcaccta
     7141 aatgctagag ctcgctgatc agcctcgact gtgccttcta gttgccagcc atctgttgtt
     7201 tgcccctccc ccgtgccttc cttgaccctg gaaggtgcca ctcccactgt cctttcctaa
     7261 taaaatgagg aaattgcatc gcattgtctg agtaggtgtc attctattct ggggggtggg
     7321 gtggggcagg acagcaaggg ggaggattgg gaagacaata gcaggcatgc tggggatgcg
     7381 gtgggctcta tggcttctga ggcggaaaga accagctggg gctctagggg gtatccccac
     7441 gcgccctgta gcggcgcatt aagcgcggcg ggtgtggtgg ttacgcgcag cgtgaccgct
     7501 acacttgcca gcgccctagc gcccgctcct ttcgctttct tcccttcctt tctcgccacg
     7561 ttcgccggct ttccccgtca agctctaaat cgggggctcc ctttagggtt ccgatttagt
     7621 gctttacggc acctcgaccc caaaaaactt gattagggtg atggttcacg tagtgggcca
     7681 tcgccctgat agacggtttt tcgccctttg acgttggagt ccacgttctt taatagtgga
     7741 ctcttgttcc aaactggaac aacactcaac cctatctcgg tctattcttt tgatttataa
     7801 gggattttgc cgatttcggc ctattggtta aaaaatgagc tgatttaaca aaaatttaac
     7861 gcgaattaat tctgtggaat gtgtgtcagt tagggtgtgg aaagtcccca ggctccccag
     7921 caggcagaag tatgcaaagc atgcatctca attagtcagc aaccaggtgt ggaaagtccc
     7981 caggctcccc agcaggcaga agtatgcaaa gcatgcatct caattagtca gcaaccatag
     8041 tcccgcccct aactccgccc atcccgcccc taactccgcc cagttccgcc cattctccgc
     8101 cccatggctg actaattttt tttatttatg cagaggccga ggccgcctct gcctctgagc
     8161 tattccagaa gtagtgagga ggcttttttg gaggcctagg cttttgcaaa aagctcccgg
     8221 gagcttgtat atccattttc ggatctgatc aagagacagg atgaggatcg tttcgcatga
     8281 ttgaacaaga tggattgcac gcaggttctc cggccgcttg ggtggagagg ctattcggct
     8341 atgactgggc acaacagaca atcggctgct ctgatgccgc cgtgttccgg ctgtcagcgc
     8401 aggggcgccc ggttcttttt gtcaagaccg acctgtccgg tgccctgaat gaactgcagg
     8461 acgaggcagc gcggctatcg tggctggcca cgacgggcgt tccttgcgca gctgtgctcg
     8521 acgttgtcac tgaagcggga agggactggc tgctattggg cgaagtgccg gggcaggatc
     8581 tcctgtcatc tcaccttgct cctgccgaga aagtatccat catggctgat gcaatgcggc
     8641 ggctgcatac gcttgatccg gctacctgcc cattcgacca ccaagcgaaa catcgcatcg
     8701 agcgagcacg tactcggatg gaagccggtc ttgtcgatca ggatgatctg gacgaagagc
     8761 atcaggggct cgcgccagcc gaactgttcg ccaggctcaa ggcgcgcatg cccgacggcg
     8821 aggatctcgt cgtgacccat ggcgatgcct gcttgccgaa tatcatggtg gaaaatggcc
     8881 gcttttctgg attcatcgac tgtggccggc tgggtgtggc ggaccgctat caggacatag
     8941 cgttggctac ccgtgatatt gctgaagagc ttggcggcga atgggctgac cgcttcctcg
     9001 tgctttacgg tatcgccgct cccgattcgc agcgcatcgc cttctatcgc cttcttgacg
     9061 agttcttctg agcgggactc tggggttcga aatgaccgac caagcgacgc ccaacctgcc
     9121 atcacgagat ttcgattcca ccgccgcctt ctatgaaagg ttgggcttcg gaatcgtttt
     9181 ccgggacgcc ggctggatga tcctccagcg cggggatctc atgctggagt tcttcgccca
     9241 ccccaacttg tttattgcag cttataatgg ttacaaataa agcaatagca tcacaaattt
     9301 cacaaataaa gcattttttt cactgcattc tagttgtggt ttgtccaaac tcatcaatgt
     9361 atcttatcat gtctgtatac cgtcgacctc tagctagagc ttggcgtaat catggtcata
     9421 gctgtttcct gtgtgaaatt gttatccgct cacaattcca cacaacatac gagccggaag
     9481 cataaagtgt aaagcctggg gtgcctaatg agtgagctaa ctcacattaa ttgcgttgcg
     9541 ctcactgccc gctttccagt cgggaaacct gtcgtgccag ctgcattaat gaatcggcca
     9601 acgcgcgggg agaggcggtt tgcgtattgg gcgctcttcc gcttcctcgc tcactgactc
     9661 gctgcgctcg gtcgttcggc tgcggcgagc ggtatcagct cactcaaagg cggtaatacg
     9721 gttatccaca gaatcagggg ataacgcagg aaagaacatg tgagcaaaag gccagcaaaa
     9781 ggccaggaac cgtaaaaagg ccgcgttgct ggcgtttttc cataggctcc gcccccctga
     9841 cgagcatcac aaaaatcgac gctcaagtca gaggtggcga aacccgacag gactataaag
     9901 ataccaggcg tttccccctg gaagctccct cgtgcgctct cctgttccga ccctgccgct
     9961 taccggatac ctgtccgcct ttctcccttc gggaagcgtg gcgctttctc atagctcacg
    10021 ctgtaggtat ctcagttcgg tgtaggtcgt tcgctccaag ctgggctgtg tgcacgaacc
    10081 ccccgttcag cccgaccgct gcgccttatc cggtaactat cgtcttgagt ccaacccggt
    10141 aagacacgac ttatcgccac tggcagcagc cactggtaac aggattagca gagcgaggta
    10201 tgtaggcggt gctacagagt tcttgaagtg gtggcctaac tacggctaca ctagaagaac
    10261 agtatttggt atctgcgctc tgctgaagcc agttaccttc ggaaaaagag ttggtagctc
    10321 ttgatccggc aaacaaacca ccgctggtag cggtggtttt tttgtttgca agcagcagat
    10381 tacgcgcaga aaaaaaggat ctcaagaaga tcctttgatc ttttctacgg ggtctgacgc
    10441 tcagtggaac gaaaactcac gttaagggat tttggtcatg agattatcaa aaaggatctt
    10501 cacctagatc cttttaaatt aaaaatgaag ttttaaatca atctaaagta tatatgagta
    10561 aacttggtct gacagttacc aatgcttaat cagtgaggca cctatctcag cgatctgtct
    10621 atttcgttca tccatagttg cctgactccc cgtcgtgtag ataactacga tacgggaggg
    10681 cttaccatct ggccccagtg ctgcaatgat accgcgagac ccacgctcac cggctccaga
    10741 tttatcagca ataaaccagc cagccggaag ggccgagcgc agaagtggtc ctgcaacttt
    10801 atccgcctcc atccagtcta ttaattgttg ccgggaagct agagtaagta gttcgccagt
    10861 taatagtttg cgcaacgttg ttgccattgc tacaggcatc gtggtgtcac gctcgtcgtt
    10921 tggtatggct tcattcagct ccggttccca acgatcaagg cgagttacat gatcccccat
    10981 gttgtgcaaa aaagcggtta gctccttcgg tcctccgatc gttgtcagaa gtaagttggc
    11041 cgcagtgtta tcactcatgg ttatggcagc actgcataat tctcttactg tcatgccatc
    11101 cgtaagatgc ttttctgtga ctggtgagta ctcaaccaag tcattctgag aatagtgtat
    11161 gcggcgaccg agttgctctt gcccggcgtc aatacgggat aataccgcgc cacatagcag
    11221 aactttaaaa gtgctcatca ttggaaaacg ttcttcgggg cgaaaactct caaggatctt
    11281 accgctgttg agatccagtt cgatgtaacc cactcgtgca cccaactgat cttcagcatc
    11341 ttttactttc accagcgttt ctgggtgagc aaaaacagga aggcaaaatg ccgcaaaaaa
    11401 gggaataagg gcgacacgga aatgttgaat actcatactc ttcctttttc aatattattg
    11461 aagcatttat cagggttatt gtctcatgag cggatacata tttgaatgta tttagaaaaa
    11521 taaacaaata ggggttccgc gcacatttc
//
