LOCUS       sequence-410959-       12172 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses FLAG-tagged PEmax (lacking the RNAseH domain) fused to Gag
            through a linker sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 12172)
  AUTHORS   Haldrup J, Andersen S, Labial ARL, Wolff JH, Frandsen FP, Skov TW, 
            Rovsing AB, Nielsen I, Jakobsen TS, Askou AL, Thomsen MK, Corydon 
            TJ, Thomsen EA, Mikkelsen JG
  TITLE     Engineered lentivirus-derived nanoparticles (LVNPs) for delivery of 
            CRISPR/Cas ribonucleoprotein complexes supporting base editing, 
            prime editing and in vivo gene modification.
  JOURNAL   Nucleic Acids Res. 2023 Sep 8:gkad676. doi: 10.1093/nar/gkad676.
  PUBMED    37678882
REFERENCE   2  (bases 1 to 12172)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nucleic 
            Acids Res. 2023 Sep 8:gkad676. doi: 10.1093/nar/gkad676."
COMMENT     Sequence Label: pLVNP3.0-PEmaxRH
FEATURES             Location/Qualifiers
     source          1..12172
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(40..59)
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     enhancer        161..540
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        541..744
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     695..715
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     741..765
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     intron          878..1353
                     /label=beta-globin intron
                     /note="internally truncated intron from human beta-globin"
     misc_feature    1419..1475
                     /label=MCS
                     /note="multiple cloning site"
     regulatory      1476..1485
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             3315..3380
                     /codon_start=1
                     /product="three tandem FLAG(R) epitope tags, followed by an
                     enterokinase cleavage site"
                     /label=3xFLAG
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
     CDS             3420..3440
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             3441..7541
                     /codon_start=1
                     /product="Cas9 (Csn1) endonuclease from the Streptococcus 
                     pyogenes Type II CRISPR/Cas system"
                     /label=Cas9
                     /note="generates RNA-guided double strand breaks in DNA"
                     /translation="DKKYSIGLDIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKKN
                     LIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEESF
                     LVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIKF
                     RGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRKLE
                     NLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQI
                     GDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVRQ
                     QLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLKREDLLR
                     KQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARGN
                     SRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEYF
                     TVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFDS
                     VEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEERL
                     KTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFMQ
                     LIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGRH
                     KPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLYL
                     YYLQNGRDMYVDQELDINRLSDYDVDAIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVPS
                     EEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKHV
                     AQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYLN
                     AVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKTE
                     ITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSKE
                     SILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGIT
                     IMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNEL
                     ALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILADA
                     NLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVLD
                     ATLIHQSITGLYETRIDLSQLGGD"
     CDS             7599..7619
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             9099..9119
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             9129..9155
                     /codon_start=1
                     /product="nuclear localization signal of human c-Myc 
                     proto-oncogene (Dang and Lee, 1988)"
                     /label=c-myc NLS
                     /translation="PAAKRVKLD"
     misc_feature    9164..9397
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     polyA_signal    9514..9908
                     /label=beta-globin poly(A)
                     /note="human beta-globin polyadenylation signal"
     primer_bind     complement(10247..10264)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(10418..11006)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(10498..10517)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(11177..11968,11969..12037))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     11800..11819
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(12038..12142)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 ggatcccctg agggggcccc catgggctag aggatccggc ctcggcctct gcataaataa
       61 aaaaaattag tcagccatga gcttggccca ttgcatacgt tgtatccata tcataatatg
      121 tacatttata ttggctcatg tccaacatta ccgccatgtt gacattgatt attgactagt
      181 tattaatagt aatcaattac ggggtcatta gttcatagcc catatatgga gttccgcgtt
      241 acataactta cggtaaatgg cccgcctggc tgaccgccca acgacccccg cccattgacg
      301 tcaataatga cgtatgttcc catagtaacg ccaataggga ctttccattg acgtcaatgg
      361 gtggagtatt tacggtaaac tgcccacttg gcagtacatc aagtgtatca tatgccaagt
      421 acgcccccta ttgacgtcaa tgacggtaaa tggcccgcct ggcattatgc ccagtacatg
      481 accttatggg actttcctac ttggcagtac atctacgtat tagtcatcgc tattaccatg
      541 gtgatgcggt tttggcagta catcaatggg cgtggatagc ggtttgactc acggggattt
      601 ccaagtctcc accccattga cgtcaatggg agtttgtttt ggcaccaaaa tcaacgggac
      661 tttccaaaat gtcgtaacaa ctccgcccca ttgacgcaaa tgggcggtag gcgtgtacgg
      721 tgggaggtct atataagcag agctcgttta gtgaaccgtc agatcgcctg gagacgccat
      781 ccacgctgtt ttgacctcca tagaagacac cgggaccgat ccagcctccc ctcgaagctt
      841 acatgtggta ccgagctcgg atcctgagaa cttcagggtg agtctatggg acccttgatg
      901 ttttctttcc ccttcttttc tatggttaag ttcatgtcat aggaagggga gaagtaacag
      961 ggtacacata ttgaccaaat cagggtaatt ttgcatttgt aattttaaaa aatgctttct
     1021 tcttttaata tacttttttg tttatcttat ttctaatact ttccctaatc tctttctttc
     1081 agggcaataa tgatacaatg tatcatgcct ctttgcacca ttctaaagaa taacagtgat
     1141 aatttctggg ttaaggcaat agcaatattt ctgcatataa atatttctgc atataaattg
     1201 taactgatgt aagaggtttc atattgctaa tagcagctac aatccagcta ccattctgct
     1261 tttattttat ggttgggata aggctggatt attctgagtc caagctaggc ccttttgcta
     1321 atcatgttca tacctcttat cttcctccca cagctcctgg gcaacgtgct ggtctgtgtg
     1381 ctggcccatc actttggcaa agcacgtgag atctgctcag atctcgagct caagcttcga
     1441 attctgcagt cgacggtacc gcgggcccgg gatccgccac catgggtgcc agggccagcg
     1501 tgctgagcgg cggcgagctg gacaggtggg agaagatcag gctgaggccc ggcggcaaga
     1561 agaagtataa gctgaagcac atcgtgtggg ccagcaggga gctggagagg ttcgccgtga
     1621 accccggcct gctggagacc agcgagggct gcaggcagat cctgggccag ctgcagccca
     1681 gcctgcagac cggcagcgag gagctgagga gcctgtacaa caccgtggcc accctgtact
     1741 gcgtgcacca gaggatcgag atcaaggaca ccaaggaggc cctggacaag atcgaggagg
     1801 agcagaacaa gtccaagaag aaggcccagc aggccgccgc cgacaccggc cacagcagcc
     1861 aggtgagcca gaactacccc atcgtgcaga acatccaggg ccagatggtg caccaggcca
     1921 tcagccccag gaccctgaac gcctgggtga aggtggtgga ggagaaggcc ttcagccccg
     1981 aggtgatccc catgttcagc gccctgagcg agggagccac cccccaggac ctgaacacca
     2041 tgctgaacac cgtgggcggc caccaggccg ccatgcagat gctgaaggag accatcaacg
     2101 aggaggccgc cgagtgggac agggtgcacc ccgtgcacgc cggccccatc gcccccggcc
     2161 agatgaggga gccccgcggc agcgacatcg ccggcaccac cagcaccctg caggagcaga
     2221 tcggctggat gaccaacaac ccccccatcc ccgtgggcga aatctacaag aggtggatca
     2281 tcctgggcct gaacaagatc gtgaggatgt acagccccac cagcatcctg gatatcaggc
     2341 agggccccaa agagcccttc agggactacg tggacaggtt ctacaagacc ctgcgcgccg
     2401 agcaggccag ccaggaggtg aagaactgga tgaccgagac cctgctggtg cagaacgcca
     2461 accccgactg caagaccatc ctgaaggccc tgggacccgc cgccaccctg gaggagatga
     2521 tgaccgcctg ccagggcgtg ggcggccccg gccacaaggc cagggtgctg gccgaggcca
     2581 tgagccaggt gaccaacacc gccaccatca tgatgcagag gggcaacttc aggaaccaga
     2641 ggaagatggt gaagtgcttc aactgcggca aggagggcca caccgccagg aactgccgct
     2701 cccccaggaa gaagggctgc tggaagtgcg gcaaggaggg ccaccagatg aaggactgca
     2761 ccgagaggca ggctaatttt ttagggaaga tctggccttc ctacaaggga aggccaggga
     2821 attttcttca gagcagacca gagccaacag ccccaccatt tcttcagagc agaccagagc
     2881 caacagcccc accagaagag agcttcaggt ctggggtaga gacaacaact ccccctcaga
     2941 agcaggagcc gatagacaag gaactgtatc ctttaacttc cctcagatca ctctttggca
     3001 acgacccctc gtcacaatta agatcggtgg ccagctcaag gaggccctgc tggacaccgg
     3061 cgccgacgac accgtgctgg aggagatgtc cctgcccggc aggtggaagc ccaagatcat
     3121 cggcggcatc ggcggcttca tcaaggtcag gcagtacgac cagatcctca tcgagatctg
     3181 cggccacaag gccatcggca ccgtgctggt gggacctaca cctgtcaaca tcatcggcag
     3241 gaacctgctc acccagatcg gctgcaccct caacttctaa ctttagccag aactatccga
     3301 ttgtgcagac cggagactat aaggaccacg acggagacta caaggatcat gatattgatt
     3361 acaaagacga tgacgataag tccatgaaac ggacagccga cggaagcgag ttcgagtcac
     3421 caaagaagaa gcggaaagtc gacaagaagt acagcatcgg cctggacatc ggcaccaact
     3481 ctgtgggctg ggccgtgatc accgacgagt acaaggtgcc cagcaagaaa ttcaaggtgc
     3541 tgggcaacac cgaccggcac agcatcaaga agaacctgat cggagccctg ctgttcgaca
     3601 gcggcgaaac agccgaggcc acccggctga agagaaccgc cagaagaaga tacaccagac
     3661 ggaagaaccg gatctgctat ctgcaagaga tcttcagcaa cgagatggcc aaggtggacg
     3721 acagcttctt ccacagactg gaagagtcct tcctggtgga agaggataag aagcacgagc
     3781 ggcaccccat cttcggcaac atcgtggacg aggtggccta ccacgagaag taccccacca
     3841 tctaccacct gagaaagaaa ctggtggaca gcaccgacaa ggccgacctg cggctgatct
     3901 atctggccct ggcccacatg atcaagttcc ggggccactt cctgatcgag ggcgacctga
     3961 accccgacaa cagcgacgtg gacaagctgt tcatccagct ggtgcagacc tacaaccagc
     4021 tgttcgagga aaaccccatc aacgccagcg gcgtggacgc caaggccatc ctgtctgcca
     4081 gactgagcaa gagcagaaag ctggaaaatc tgatcgccca gctgcccggc gagaagaaga
     4141 atggcctgtt cggaaacctg attgccctga gcctgggcct gacccccaac ttcaagagca
     4201 acttcgacct ggccgaggat gccaaactgc agctgagcaa ggacacctac gacgacgacc
     4261 tggacaacct gctggcccag atcggcgacc agtacgccga cctgtttctg gccgccaaga
     4321 acctgtccga cgccatcctg ctgagcgaca tcctgagagt gaacaccgag atcaccaagg
     4381 cccccctgag cgcctctatg atcaagagat acgacgagca ccaccaggac ctgaccctgc
     4441 tgaaagctct cgtgcggcag cagctgcctg agaagtacaa agagattttc ttcgaccaga
     4501 gcaagaacgg ctacgccggc tacattgacg gcggagccag ccaggaagag ttctacaagt
     4561 tcatcaagcc catcctggaa aagatggacg gcaccgagga actgctcgtg aagctgaaga
     4621 gagaggacct gctgcggaag cagcggacct tcgacaacgg cagcatcccc caccagatcc
     4681 acctgggaga gctgcacgcc attctgcggc ggcaggaaga tttttaccca ttcctgaagg
     4741 acaaccggga aaagatcgag aagatcctga ccttccgcat cccctactac gtgggccctc
     4801 tggccagggg aaacagcaga ttcgcctgga tgaccagaaa gagcgaggaa accatcaccc
     4861 cctggaactt cgaggaagtg gtggacaagg gcgcttccgc ccagagcttc atcgagcgga
     4921 tgaccaactt cgataagaac ctgcccaacg agaaggtgct gcccaagcac agcctgctgt
     4981 acgagtactt caccgtgtat aacgagctga ccaaagtgaa atacgtgacc gagggaatga
     5041 gaaagcccgc cttcctgagc ggcgagcaga aaaaggccat cgtggacctg ctgttcaaga
     5101 ccaaccggaa agtgaccgtg aagcagctga aagaggacta cttcaagaaa atcgagtgct
     5161 tcgactccgt ggaaatctcc ggcgtggaag atcggttcaa cgcctccctg ggcacatacc
     5221 acgatctgct gaaaattatc aaggacaagg acttcctgga caatgaggaa aacgaggaca
     5281 ttctggaaga tatcgtgctg accctgacac tgtttgagga cagagagatg atcgaggaac
     5341 ggctgaaaac ctatgcccac ctgttcgacg acaaagtgat gaagcagctg aagcggcgga
     5401 gatacaccgg ctggggcagg ctgagccgga agctgatcaa cggcatccgg gacaagcagt
     5461 ccggcaagac aatcctggat ttcctgaagt ccgacggctt cgccaacaga aacttcatgc
     5521 agctgatcca cgacgacagc ctgaccttta aagaggacat ccagaaagcc caggtgtccg
     5581 gccagggcga tagcctgcac gagcacattg ccaatctggc cggcagcccc gccattaaga
     5641 agggcatcct gcagacagtg aaggtggtgg acgagctcgt gaaagtgatg ggccggcaca
     5701 agcccgagaa catcgtgatc gaaatggcca gagagaacca gaccacccag aagggacaga
     5761 agaacagccg cgagagaatg aagcggatcg aagagggcat caaagagctg ggcagccaga
     5821 tcctgaaaga acaccccgtg gaaaacaccc agctgcagaa cgagaagctg tacctgtact
     5881 acctgcagaa tgggcgggat atgtacgtgg accaggaact ggacatcaac cggctgtccg
     5941 actacgatgt ggacgctatc gtgcctcaga gctttctgaa ggacgactcc atcgacaaca
     6001 aggtgctgac cagaagcgac aagaaccggg gcaagagcga caacgtgccc tccgaagagg
     6061 tcgtgaagaa gatgaagaac tactggcggc agctgctgaa cgccaagctg attacccaga
     6121 gaaagttcga caatctgacc aaggccgaga gaggcggcct gagcgaactg gataaggccg
     6181 gcttcatcaa gagacagctg gtggaaaccc ggcagatcac aaagcacgtg gcacagatcc
     6241 tggactcccg gatgaacact aagtacgacg agaatgacaa gctgatccgg gaagtgaaag
     6301 tgatcaccct gaagtccaag ctggtgtccg atttccggaa ggatttccag ttttacaaag
     6361 tgcgcgagat caacaactac caccacgccc acgacgccta cctgaacgcc gtcgtgggaa
     6421 ccgccctgat caaaaagtac cctaagctgg aaagcgagtt cgtgtacggc gactacaagg
     6481 tgtacgacgt gcggaagatg atcgccaaga gcgagcagga aatcggcaag gctaccgcca
     6541 agtacttctt ctacagcaac atcatgaact ttttcaagac cgagattacc ctggccaacg
     6601 gcgagatccg gaagcggcct ctgatcgaga caaacggcga aaccggggag atcgtgtggg
     6661 ataagggccg ggattttgcc accgtgcgga aagtgctgag catgccccaa gtgaatatcg
     6721 tgaaaaagac cgaggtgcag acaggcggct tcagcaaaga gtctatcctg cccaagagga
     6781 acagcgataa gctgatcgcc agaaagaagg actgggaccc taagaagtac ggcggcttcg
     6841 acagccccac cgtggcctat tctgtgctgg tggtggccaa agtggaaaag ggcaagtcca
     6901 agaaactgaa gagtgtgaaa gagctgctgg ggatcaccat catggaaaga agcagcttcg
     6961 agaagaatcc catcgacttt ctggaagcca agggctacaa agaagtgaaa aaggacctga
     7021 tcatcaagct gcctaagtac tccctgttcg agctggaaaa cggccggaag agaatgctgg
     7081 cctctgccgg cgaactgcag aagggaaacg aactggccct gccctccaaa tatgtgaact
     7141 tcctgtacct ggccagccac tatgagaagc tgaagggctc ccccgaggat aatgagcaga
     7201 aacagctgtt tgtggaacag cacaagcact acctggacga gatcatcgag cagatcagcg
     7261 agttctccaa gagagtgatc ctggccgacg ctaatctgga caaagtgctg tccgcctaca
     7321 acaagcaccg ggataagccc atcagagagc aggccgagaa tatcatccac ctgtttaccc
     7381 tgaccaatct gggagcccct gccgccttca agtactttga caccaccatc gaccggaaga
     7441 ggtacaccag caccaaagag gtgctggacg ccaccctgat ccaccagagc atcaccggcc
     7501 tgtacgagac acggatcgac ctgtctcagc tgggaggtga ctccggcgga agctctggtg
     7561 gcagcaagcg gaccgccgac ggctctgaat tcgagagccc taagaagaaa agaaaggtga
     7621 gcggaggctc tagcggcgga agcctgggct ccacctggct gtccgacttt ccccaggcct
     7681 gggccgagac aggaggaatg ggcctggccg tgcggcaggc acccctgatc atccctctga
     7741 aggccacctc tacacccgtg agcatcaagc agtaccctat gtctcaggag gccagactgg
     7801 gcatcaagcc tcacatccag aggctgctgg accagggcat cctggtgcca tgccagagcc
     7861 cctggaacac accactgctg cccgtgaaga agccaggcac caatgactat agacccgtgc
     7921 aggatctgag agaggtgaac aagagggtgg aggatatcca ccccaccgtg cccaaccctt
     7981 acaatctgct gtccggcctg cccccttctc accagtggta tacagtgctg gacctgaagg
     8041 atgccttctt ttgtctgaga ctgcacccta ccagccagcc actgttcgcc tttgagtgga
     8101 gggaccctga gatgggcatc tctggccagc tgacctggac acgcctgcct cagggcttca
     8161 agaatagccc aacactgttt aacgaggccc tgcaccgcga cctggcagat ttccggatcc
     8221 agcacccaga tctgatcctg ctgcagtacg tggacgatct gctgctggcc gccaccagcg
     8281 agctggattg ccagcaggga acacgcgccc tgctgcagac cctgggaaac ctgggatata
     8341 gggcatccgc caagaaggcc cagatctgtc agaagcaggt gaagtacctg ggctatctgc
     8401 tgaaggaggg ccagagatgg ctgacagagg ccaggaagga gacagtgatg ggccagccaa
     8461 cacccaagac cccaagacag ctgagggagt tcctgggcaa agcaggattt tgcaggctgt
     8521 tcatcccagg attcgcagag atggcagcac ctctgtaccc actgaccaag ccgggcaccc
     8581 tgtttaattg gggccctgac cagcagaagg cctatcagga gatcaagcag gccctgctga
     8641 cagcaccagc cctgggcctg ccagacctga ccaagccttt cgagctgttt gtggatgaga
     8701 agcagggcta cgccaagggc gtgctgaccc agaagctggg accatggaga cggcccgtgg
     8761 cctatctgtc caagaagctg gacccagtgg cagcaggatg gccaccatgc ctgaggatgg
     8821 tggcagcaat cgccgtgctg acaaaggatg ccggcaagct gaccatggga cagccactgg
     8881 tcatcctggc accacacgca gtggaggccc tggtgaagca gcctccagat cgctggctgt
     8941 ctaacgcccg gatgacacac taccaggccc tgctgctgga caccgatcgc gtgcagtttg
     9001 gccctgtggt ggccctgaat ccagccaccc tgctgcctct gccagaggag ggctctggcg
     9061 gctcaaaaag aaccgccgac ggcagcgaat tcgagtctcc caagaagaag aggaaagtcg
     9121 gctctggccc tgccgctaag agagtgaagc tggactaagc cgcaggagct ttgttccttg
     9181 ggttcttggg agcagcagga agcactatgg gcgcagcgtc aatgacgctg acggtacagg
     9241 ccagacaatt attgtctggt atagtgcagc agcagaacaa tttgctgagg gctattgagg
     9301 cgcaacagca tctgttgcaa ctcacagtct ggggcatcaa gcagctccag gcaagaatcc
     9361 tggctgtgga aagataccta aaggatcaac agctcctggg gatttggggt tgctctggaa
     9421 aactcatttg caataaacac cccaccagtg caggctgcct atcagaaagt ggtggctggt
     9481 gtggctaatg ccctggccca caagtatcac taagctcgct ttcttgctgt ccaatttcta
     9541 ttaaaggttc ctttgttccc taagtccaac tactaaactg ggggatatta tgaagggcct
     9601 tgagcatctg gattctgcct aataaaaaac atttattttc attgcaatga tgtatttaaa
     9661 ttatttctga atattttact aaaaagggaa tgtgggaggt cagtgcattt aaaacataaa
     9721 gaaatgaaga gctagttcaa accttgggaa aatacactat atcttaaact ccatgaaaga
     9781 aggtgaggct gcaaacagct aatgcacatt ggcaacagcc cctgatgcct atgccttatt
     9841 catccctcag aaaaggattc aagtagaggc ttgatttgga ggttaaagtt ttgctatgct
     9901 gtattttaca ttacttattg ttttagctgt cctcatgaat gtcttttcac tacccatttg
     9961 cttatcctgc atctctcagc cttgactcca ctcagttctc ttgcttagag ataccacctt
    10021 tcccctgaag tgttccttcc atgttttacg gcgagatggt ttctcctcgc ctggccactc
    10081 agccttagtt gtctctgttg tcttatagag gtctacttga agaaggaaaa acagggggca
    10141 tggtttgact gtcctgtgag cccttcttcc ctgcctcccc cactcacagt gacccggaat
    10201 ccctcgacat ggcagtctag cactagtgcg gccgcagatc tgcttcctcg ctcactgact
    10261 cgctgcgctc ggtcgttcgg ctgcggcgag cggtatcagc tcactcaaag gcggtaatac
    10321 ggttatccac agaatcaggg gataacgcag gaaagaacat gtgagcaaaa ggccagcaaa
    10381 aggccaggaa ccgtaaaaag gccgcgttgc tggcgttttt ccataggctc cgcccccctg
    10441 acgagcatca caaaaatcga cgctcaagtc agaggtggcg aaacccgaca ggactataaa
    10501 gataccaggc gtttccccct ggaagctccc tcgtgcgctc tcctgttccg accctgccgc
    10561 ttaccggata cctgtccgcc tttctccctt cgggaagcgt ggcgctttct catagctcac
    10621 gctgtaggta tctcagttcg gtgtaggtcg ttcgctccaa gctgggctgt gtgcacgaac
    10681 cccccgttca gcccgaccgc tgcgccttat ccggtaacta tcgtcttgag tccaacccgg
    10741 taagacacga cttatcgcca ctggcagcag ccactggtaa caggattagc agagcgaggt
    10801 atgtaggcgg tgctacagag ttcttgaagt ggtggcctaa ctacggctac actagaagaa
    10861 cagtatttgg tatctgcgct ctgctgaagc cagttacctt cggaaaaaga gttggtagct
    10921 cttgatccgg caaacaaacc accgctggta gcggtggttt ttttgtttgc aagcagcaga
    10981 ttacgcgcag aaaaaaagga tctcaagaag atcctttgat cttttctacg gggtctgacg
    11041 ctcagtggaa cgaaaactca cgttaaggga ttttggtcat gagattatca aaaaggatct
    11101 tcacctagat ccttttaaat taaaaatgaa gttttaaatc aatctaaagt atatatgagt
    11161 aaacttggtc tgacagttac caatgcttaa tcagtgaggc acctatctca gcgatctgtc
    11221 tatttcgttc atccatagtt gcctgactcc ccgtcgtgta gataactacg atacgggagg
    11281 gcttaccatc tggccccagt gctgcaatga taccgcgaga cccacgctca ccggctccag
    11341 atttatcagc aataaaccag ccagccggaa gggccgagcg cagaagtggt cctgcaactt
    11401 tatccgcctc catccagtct attaattgtt gccgggaagc tagagtaagt agttcgccag
    11461 ttaatagttt gcgcaacgtt gttgccattg ctacaggcat cgtggtgtca cgctcgtcgt
    11521 ttggtatggc ttcattcagc tccggttccc aacgatcaag gcgagttaca tgatccccca
    11581 tgttgtgcaa aaaagcggtt agctccttcg gtcctccgat cgttgtcaga agtaagttgg
    11641 ccgcagtgtt atcactcatg gttatggcag cactgcataa ttctcttact gtcatgccat
    11701 ccgtaagatg cttttctgtg actggtgagt actcaaccaa gtcattctga gaatagtgta
    11761 tgcggcgacc gagttgctct tgcccggcgt caatacggga taataccgcg ccacatagca
    11821 gaactttaaa agtgctcatc attggaaaac gttcttcggg gcgaaaactc tcaaggatct
    11881 taccgctgtt gagatccagt tcgatgtaac ccactcgtgc acccaactga tcttcagcat
    11941 cttttacttt caccagcgtt tctgggtgag caaaaacagg aaggcaaaat gccgcaaaaa
    12001 agggaataag ggcgacacgg aaatgttgaa tactcatact cttccttttt caatattatt
    12061 gaagcattta tcagggttat tgtctcatga gcggatacat atttgaatgt atttagaaaa
    12121 ataaacaaat aggggttccg cgcacatttc cccgaaaagt gccacctgac gt
//
