LOCUS       sequence-410967-        4850 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Direct-expressing SYFP2 in oligodendrocytes AAV Virus.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4850)
  AUTHORS   Ben-Simon Y, Hooper M, Narayan S, Daigle TL, Dwivedi D, Way SW, 
            Oster A, Stafford DA, Mich JK, Taormina MJ, Martinez RA, Opitz-Araya
            X, Roth JR, Alexander JR, Allen S, Amster A, Arbuckle J, Ayala A, 
            Baker PM, Bakken TE, Barcelli T, Barta S, Bendrick J, Bertagnolli D,
            Bielstein C, Bishwakarma P, Bowlus J, Boyer G, Brouner K, Casian B, 
            Casper T, Chakka AB, Chakrabarty R, Chance RK, Chavan S, Clark M, 
            Colbert K, Collman F, Daniel S, Departee M, DiValentin P, Donadio N,
            Dotson N, Egdorf T, Fliss T, Gabitto M, Garcia J, Gary A, Gasperini 
            M, Gloe J, Goldy J, Gore BB, Graybuck L, Greisman N, Haeseleer F, 
            Halterman C, Haradon Z, Hastings SD, Helback O, Ho W, Hockemeyer D, 
            Huang C, Huff S, Hunker A, Johansen N, Jones D, Juneau Z, Kalmbach 
            B, Kannan M, Khem S, Kussick E, Kutsal R, Larsen R, Lee C, Lee AY, 
            Leibly M, Lenz GH, Li S, Liang E, Lusk N, Madigan Z, Malloy J, 
            Malone J, McCue R, Melchor J, Mollenkopf T, Moosman S, Morin E, 
            Newman D, Ng L, Ngo K, Omstead V, Otto S, Oyama A, Pena N, Pham T, 
            Phillips E, Pom CA, Potekhina L, Ransford S, Ray PL, Rette D, 
            Reynoldson C, Rimorin C, Rocha D, Ruiz A, Sanchez REA, Sawyer L, 
            Sedeno-Cortes A, Sevigny JP, Shapovalova N, Shepard N, Shulga L, 
            Sigler AR, Siverts L, Soliman S, Somasundaram S, Staats B, Stewart 
            K, Szelenyi E, Tieu M, Trader C, Tran A, van Velthoven CTJ, Walker 
            M, Wang Y, Weed N, Wirthlin M, Wood T, Wynalda B, Yao Z, Zhou T, 
            Ariza J, Dee N, Reding M, Ronellenfitch K, Mufti S, Sunkin SM, Smith
            KA, Esposito L, Waters J, Thyagarajan B, Yao S, Lein ES, Zeng H, 
            Levi BP, Ngai J, Ting JT, Tasic B
  TITLE     A suite of enhancer AAVs and transgenic mouse lines for genetic 
            access to cortical cell types.
  JOURNAL   Cell. 2025 May 16:S0092-8674(25)00513-6. doi: 
            10.1016/j.cell.2025.05.002.
  PUBMED    40403729
REFERENCE   2  (bases 1 to 4850)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell. 2025 
            May 16:S0092-8674(25)00513-6. doi: 10.1016/j.cell.2025.05.002."
COMMENT     Sequence Label: AiP12157 -
            pAAV-AiE0395h-minBglobin-SYFP2-WPRE...
FEATURES             Location/Qualifiers
     source          1..4850
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     repeat_region   1..130
                     /label=AAV2 ITR (alternate)
                     /note="Functional equivalent of wild-type AAV2 ITR"
     regulatory      862..871
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(868..870,871..873,874..1584)
                     /codon_start=1
                     /product="monomeric optimized variant of EYFP, identical to
                     mVenus but with L68V (Kremers et al., 2006)"
                     /label=SYFP2
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KLICTTGKLPVPWPTLVTTLGYGVQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYITADKQKNGIK
                     ANFKIRHNIEDGGVQLADHYQQNTPIGDGPVLLPDNHYLSYQSKLSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(913..934)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(1174..1193)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     1521..1542
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     primer_bind     complement(1677..1697)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     polyA_signal    1884..1995
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     repeat_region   2113..2253
                     /label=AAV2 ITR
                     /note="inverted terminal repeat of adeno-associated virus 
                     serotype 2"
     repeat_region   2113..2242
                     /label=AAV2 ITR
     rep_origin      2328..2783
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(2415..2434)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     2625..2646
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(2800..2819)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     2919..2941
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(2979..2997)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        3065..3169
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(3170..3238,3239..4030)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(3388..3407)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      4201..4789
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     4690..4709
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 cctgcaggca gctgcgcgct cgctcgctca ctgaggccgc ccgggcgtcg ggcgaccttt
       61 ggtcgcccgg cctcagtgag cgagcgagcg cgcagagagg gagtggccaa ctccatcact
      121 aggggttcct gcggccgcac gcgtctggcc ttggtctctc ctgggctaga acaggcccct
      181 tgtctgcctc cctgaatcac tcttgaggct gggcatgcac cttcccccac tccagatgtg
      241 ctgacagcag aaatgccaac ccctcccttg agggccagtg aattcagcag tgacaggtca
      301 ctctctgagc gagactggtt ctcccttcat ccgcttgtaa agggaaaaac agcctcggcc
      361 tctgagcctg gcaccaccag gtttggccag tcttggttgt ttgcctaagt cccagtgcct
      421 tcagttccca tccctcttct ctgaccccca actcctgagc cccttgcctg tcaccctccc
      481 ccaccccagc tgctgcatgt gccaaccccc cacccaggag aaaacgaggc tcttggaagg
      541 gaataaatgg aggctctgtg caggcttgca gctgaaatgg ccctgtgcgc ggccacagat
      601 gggcctcctc tccccctcca catttctgca tcaacaaagc gtgaccttgt ttggatgaca
      661 gtcgccccat gttttcccca tgacaatgct tgccttgcct ttgcctccca gcgggctgtc
      721 tcaggacatc tctgttccct gaaattggga aagggtggtg gggtggaaga gctcgggctg
      781 ggcataaaag tcagggcaga gccatctatt gcttacattt gcttctggga tccagatctt
      841 tcgaagctag cgctaccggt cgccaccatg gtgagcaagg gcgaggagct gttcaccggg
      901 gtggtgccca tcctggtcga gctggacggc gacgtaaacg gccacaagtt cagcgtgtcc
      961 ggcgagggcg agggcgatgc cacctacggc aagctgaccc tgaagctgat ctgcaccacc
     1021 ggcaagctgc ccgtgccctg gcccaccctc gtgaccaccc tgggctacgg cgtgcagtgc
     1081 ttcgcccgct accccgacca catgaagcag cacgacttct tcaagtccgc catgcccgaa
     1141 ggctacgtcc aggagcgcac catcttcttc aaggacgacg gcaactacaa gacccgcgcc
     1201 gaggtgaagt tcgagggcga caccctggtg aaccgcatcg agctgaaggg catcgacttc
     1261 aaggaggacg gcaacatcct ggggcacaag ctggagtaca actacaacag ccacaacgtc
     1321 tatatcaccg ccgacaagca gaagaacggc atcaaggcca acttcaagat ccgccacaac
     1381 atcgaggacg gcggcgtgca gctcgccgac cactaccagc agaacacccc catcggcgac
     1441 ggccccgtgc tgctgcccga caaccactac ctgagctacc agtccaagct gagcaaagac
     1501 cccaacgaga agcgcgatca catggtcctg ctggagttcg tgaccgccgc cgggatcact
     1561 ctcggcatgg acgagctgta caagtaagtc gacggcgcgc cgcggccgcg aattcgatat
     1621 cataatcaac ctctggatta caaaatttgt gaaagattga ctggtattct taactatgtt
     1681 gctcctttta cgctatgtgg atacgctgct ttaatgcctt tgtatcatgc tattgcttcc
     1741 cgtatggctt tcattttctc ctccttgtat aaatcctggt tagttcttgc cacggcggaa
     1801 ctcatcgccg cctgccttgc ccgctgctgg acaggggctc ggctgttggg cactgacaat
     1861 tccgtggctc gagagatctt cgactgtgcc ttctagttgc cagccatctg ttgtttgccc
     1921 ctcccccgtg ccttccttga ccctggaagg tgccactccc actgtccttt cctaataaaa
     1981 tgaggaaatt gcatcgcatt gtctgagtag gtgtcattct attctggggg gtggggtggg
     2041 gcaggacagc aagggggagg attgggaaga caatagcagg catgagatct cacgtgcgga
     2101 ccgagcggcc gcaggaaccc ctagtgatgg agttggccac tccctctctg cgcgctcgct
     2161 cgctcactga ggccgggcga ccaaaggtcg cccgacgccc gggctttgcc cgggcggcct
     2221 cagtgagcga gcgagcgcgc agctgcctgc aggggcgcct gatgcggtat tttctcctta
     2281 cgcatctgtg cggtatttca caccgcatac gtcaaagcaa ccatagtacg cgccctgtag
     2341 cggcgcatta agcgcggcgg gtgtggtggt tacgcgcagc gtgaccgcta cacttgccag
     2401 cgccttagcg cccgctcctt tcgctttctt cccttccttt ctcgccacgt tcgccggctt
     2461 tccccgtcaa gctctaaatc gggggctccc tttagggttc cgatttagtg ctttacggca
     2521 cctcgacccc aaaaaacttg atttgggtga tggttcacgt agtgggccat cgccctgata
     2581 gacggttttt cgccctttga cgttggagtc cacgttcttt aatagtggac tcttgttcca
     2641 aactggaaca acactcaact ctatctcggg ctattctttt gatttataag ggattttgcc
     2701 gatttcggtc tattggttaa aaaatgagct gatttaacaa aaatttaacg cgaattttaa
     2761 caaaatatta acgtttacaa ttttatggtg cactctcagt acaatctgct ctgatgccgc
     2821 atagttaagc cagccccgac acccgccaac acccgctgac gcgccctgac gggcttgtct
     2881 gctcccggca tccgcttaca gacaagctgt gaccgtctcc gggagctgca tgtgtcagag
     2941 gttttcaccg tcatcaccga aacgcgcgag acgaaagggc ctcgtgatac gcctattttt
     3001 ataggttaat gtcatgataa taatggtttc ttagacgtca ggtggcactt ttcggggaaa
     3061 tgtgcgcgga acccctattt gtttattttt ctaaatacat tcaaatatgt atccgctcat
     3121 gagacaataa ccctgataaa tgcttcaata atattgaaaa aggaagagta tgagtattca
     3181 acatttccgt gtcgccctta ttcccttttt tgcggcattt tgccttcctg tttttgctca
     3241 cccagaaacg ctggtgaaag taaaagatgc tgaagatcag ttgggtgcac gagtgggtta
     3301 catcgaactg gatctcaaca gcggtaagat ccttgagagt tttcgccccg aagaacgttt
     3361 tccaatgatg agcactttta aagttctgct atgtggcgcg gtattatccc gtattgacgc
     3421 cgggcaagag caactcggtc gccgcataca ctattctcag aatgacttgg ttgagtactc
     3481 accagtcaca gaaaagcatc ttacggatgg catgacagta agagaattat gcagtgctgc
     3541 cataaccatg agtgataaca ctgcggccaa cttacttctg acaacgatcg gaggaccgaa
     3601 ggagctaacc gcttttttgc acaacatggg ggatcatgta actcgccttg atcgttggga
     3661 accggagctg aatgaagcca taccaaacga cgagcgtgac accacgatgc ctgtagcaat
     3721 ggcaacaacg ttgcgcaaac tattaactgg cgaactactt actctagctt cccggcaaca
     3781 attaatagac tggatggagg cggataaagt tgcaggacca cttctgcgct cggcccttcc
     3841 ggctggctgg tttattgctg ataaatctgg agccggtgag cgtgggtctc gcggtatcat
     3901 tgcagcactg gggccagatg gtaagccctc ccgtatcgta gttatctaca cgacggggag
     3961 tcaggcaact atggatgaac gaaatagaca gatcgctgag ataggtgcct cactgattaa
     4021 gcattggtaa ctgtcagacc aagtttactc atatatactt tagattgatt taaaacttca
     4081 tttttaattt aaaaggatct aggtgaagat cctttttgat aatctcatga ccaaaatccc
     4141 ttaacgtgag ttttcgttcc actgagcgtc agaccccgta gaaaagatca aaggatcttc
     4201 ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc
     4261 agcggtggtt tgtttgccgg atcaagagct accaactctt tttccgaagg taactggctt
     4321 cagcagagcg cagataccaa atactgttct tctagtgtag ccgtagttag gccaccactt
     4381 caagaactct gtagcaccgc ctacatacct cgctctgcta atcctgttac cagtggctgc
     4441 tgccagtggc gataagtcgt gtcttaccgg gttggactca agacgatagt taccggataa
     4501 ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag cccagcttgg agcgaacgac
     4561 ctacaccgaa ctgagatacc tacagcgtga gctatgagaa agcgccacgc ttcccgaagg
     4621 gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga acaggagagc gcacgaggga
     4681 gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc acctctgact
     4741 tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa
     4801 cgcggccttt ttacggttcc tggccttttg ctggcctttt gctcacatgt
//
