LOCUS       sequence-410984-       15119 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Positive control for FRET in plant ER / nuclear envelope..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 15119)
  AUTHORS   McKenna JF, Gumber HK, Turpin ZM, Jalovec AM, Kartick AC, Graumann 
            K, Bass HW
  TITLE     Maize (Zea mays L.) Nucleoskeletal Proteins Regulate Nuclear 
            Envelope Remodeling and Function in Stomatal Complex Development and
            Pollen Viability.
  JOURNAL   Front Plant Sci. 2021 Feb 17;12:645218. doi: 
            10.3389/fpls.2021.645218. eCollection 2021.
  PUBMED    33679862
REFERENCE   2  (bases 1 to 15119)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Front Plant
            Sci."; date: "2021-02-17"; volume: "12:645218. doi"; pages: " 
            10.3389/fpls.2021.645218. eCollection 2021"
COMMENT     Sequence Label: p35S::SP-mCherry-GFP-HDEL
FEATURES             Location/Qualifiers
     source          1..15119
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      1..589
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     terminator      complement(919..946)
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     terminator      complement(1038..1124)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     primer_bind     1173..1195
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     1187..1204
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     1188..1204
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    1220..1319
                     /gene="mutant version of attL"
                     /label=attL1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     CDS             1386..2093
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /label=mCherry
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     CDS             join(2109..2111,2112..2114,2115..2825)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             join(2109..2111,2112..2114,2115..2825)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     protein_bind    complement(2842..2866)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             join(2874..2876,2877..2879,2880..3593)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             join(2874..2876,2877..2879,2880..3590)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     promoter        3959..4138
                     /label=NOS promoter
                     /note="nopaline synthase promoter"
     CDS             4180..4731
                     /codon_start=1
                     /gene="Streptomyces hygroscopicus bar"
                     /product="phosphinothricin acetyltransferase"
                     /label=BlpR
                     /note="confers resistance to bialophos or phosphinothricin"
                     /translation="MSPERRPADIRRATEADMPAVCTIVNHYIETSTVNFRTEPQEPQE
                     WTDDLVRLRERYPWLVAEVDGEVAGIAYAGPWKARNAYDWTAESTVYVSPRHQRTGLGS
                     TLYTHLLKSLEAQGFKSVVAVIGLPNDPSVRMHEALGYAPRGMLRAAGFKHGNWHDVGF
                     WQLDFSLPVPPRPVLPVTEI"
     terminator      4751..5003
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     misc_feature    5096..5120
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     CDS             5641..6432
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MGEAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     rep_origin      6678..7266
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     misc_feature    7452..7592
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     complement(7518..7537)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     7678..7700
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     rep_origin      7936..8130
                     /label=pVS1 oriV
                     /note="origin of replication for the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
     CDS             complement(8196..9269)
                     /codon_start=1
                     /product="replication protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 RepA
                     /translation="MSGRKPSGPVQIGAALGDDLVEKLKAAQAAQRQRIEAEARPGESW
                     QAAADRIRKESRQPPAAGAPSIRKPPKGDEQPDFFVPMLYDVGTRDSRSIMDVAVFRLS
                     KRDRRAGEVIRYELPDGHVEVSAGPAGMASVWDYDLVLMAVSHLTESMNRYREGKGDKP
                     GRVFRPHVADVLKFCRRADGGKQKDDLVETCIRLNTTHVAMQRTKKAKNGRLVTVSEGE
                     ALISRYKIVKSETGRPEYIEIELADWMYREITEGKNPDVLTVHPDYFLIDPGIGRFLYR
                     LARRAAGKAEARWLFKTIYERSGSAGEFKKFCFTVRKLIGSNDLPEYDLKEEAGQAGPI
                     LVMRYRNLIEGEASAGS"
     CDS             complement(9698..10327)
                     /codon_start=1
                     /product="stability protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 StaA
                     /translation="MKVIAVLNQKGGSGKTTIATHLARALQLAGADVLLVDSDPQGSAR
                     DWAAVREDQPLTVVGIDRPTIDRDVKAIGRRDFVVIDGAPQAADLAVSAIKAADFVLIP
                     VQPSPYDIWATADLVELVKQRIEVTDGRLQAAFVVSRAIKGTRIGGEVAEALAGYELPI
                     LESRITQRVSYPGTAAAGTTVLESEPEGDAAREVQALAAEIKSKLI"
     misc_feature    11658..11682
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     promoter        12375..12719
                     /label=CaMV 35S promoter
                     /note="strong constitutive promoter from cauliflower mosaic
                     virus"
     primer_bind     12663..12683
                     /label=35S promoter
                     /note="CaMV 35S promoter, forward primer"
     protein_bind    12819..12843
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             12910..13617
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /label=mCherry
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     CDS             join(13633..13635,13636..13638,13639..14349)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             join(13633..13635,13636..13638,13639..14349)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     protein_bind    complement(14366..14465)
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(14482..14501)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(14483..14501)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(14506..14522)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(14506..14522)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     CDS             complement(14590..14964)
                     /codon_start=1
                     /gene="Sh ble from Streptoalloteichus hindustanus"
                     /product="antibiotic-binding protein"
                     /label=BleoR
                     /note="confers resistance to bleomycin, phleomycin, and 
                     Zeocin(TM)"
                     /translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD
                     VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR
                     EFALRDPAGNCVHFVAEEQD"
     promoter        complement(14983..15030)
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
ORIGIN
        1 ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc
       61 agcggtggtt tgtttgccgg atcaagagct accaactctt tttccgaagg taactggctt
      121 cagcagagcg cagataccaa atactgttct tctagtgtag ccgtagttag gccaccactt
      181 caagaactct gtagcaccgc ctacatacct cgctctgcta atcctgttac cagtggctgc
      241 tgccagtggc gataagtcgt gtcttaccgg gttggactca agacgatagt taccggataa
      301 ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag cccagcttgg agcgaacgac
      361 ctacaccgaa ctgagatacc tacagcgtga gctatgagaa agcgccacgc ttcccgaagg
      421 gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga acaggagagc gcacgaggga
      481 gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc acctctgact
      541 tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa
      601 cgcggccttt ttacggttcc tggccttttg ctggcctttt gctcacatgt tctttcctgc
      661 gttatcccct gattctgtgg ataaccgtat taccgccttt gagtgagctg ataccgctcg
      721 ccgcagccga acgaccgagc gcagcgagtc agtgagcgag gaagcggaag agcgcccaat
      781 acgcaaaccg cctctccccg cgcgttggcc gattcattaa tgcagctggc acgacaggtt
      841 tcccgactgg aaagcgggca gtgagcgcaa cgcaattaat acgcgtaccg ctagccagga
      901 agagtttgta gaaacgcaaa aaggccatcc gtcaggatgg ccttctgctt agtttgatgc
      961 ctggcagttt atggcgggcg tcctgcccgc caccctccgg gccgttgctt cacaacgttc
     1021 aaatccgctc ccggcggatt tgtcctactc aggagagcgt tcaccgacaa acaacagata
     1081 aaacgaaagg cccagtcttc cgactgagcc tttcgtttta tttgatgcct ggcagttccc
     1141 tactctcgcg ttaacgctag catggatgtt ttcccagtca cgacgttgta aaacgacggc
     1201 cagtcttaag ctcgggcccc aaataatgat tttattttga ctgatagtga cctgttcgtt
     1261 gcaacaaatt gatgagcaat gcttttttat aatgccaact ttgtacaaaa aagcaggcta
     1321 caatgaaagc cttcacactc gctctcttct tagctctttc cctctatctc ctgcccaatc
     1381 cagccatggt gagcaagggc gaggaggata acatggccat catcaaggag ttcatgcgtt
     1441 tcaaggtgca catggagggc tccgtgaacg gccacgagtt cgagatcgag ggcgagggcg
     1501 agggccgccc ctacgagggc acccagaccg ccaagctgaa ggtgaccaag ggtggccccc
     1561 tgcccttcgc ctgggacatc ctgtcccctc agttcatgta cggctccaag gcctacgtga
     1621 agcaccccgc cgacatcccc gactacttga agctgtcctt ccccgagggc ttcaagtggg
     1681 agcgcgtgat gaacttcgag gacggcggcg tggtgaccgt gacccaggac tcctccctgc
     1741 aggacggcga gttcatctac aaggtgaagc tgcgcggcac caacttcccc tccgacggcc
     1801 ccgtaatgca gaagaagacc atgggctggg aggcctcctc cgagcggatg taccccgagg
     1861 acggcgccct gaagggcgag atcaagcaga ggctgaagct gaaggacggc ggccactacg
     1921 acgctgaggt caagaccacc tacaaggcca agaagcccgt gcagctgccc ggcgcctaca
     1981 acgtcaacat caagttggac atcacctccc acaacgagga ctacaccatc gtggaacagt
     2041 acgaacgcgc cgagggccgc cactccaccg gcggcatgga cgagctgtac aagggtggca
     2101 gtggaggtat ggtgagcaag ggcgaggagc tgttcaccgg ggtggtgccc atcctggtcg
     2161 agctggacgg cgacgtaaac ggccacaagt tcagcgtgtc cggcgagggc gagggcgatg
     2221 ccacctacgg caagctgacc ctgaagttca tctgcaccac cggcaagctg cccgtgccct
     2281 ggcccaccct cgtgaccacc ctgacctacg gcgtgcagtg cttcagccgc taccccgacc
     2341 acatgaagca gcacgacttc ttcaagtccg ccatgcccga aggctacgtc caggagcgca
     2401 ccatcttctt caaggacgac ggcaactaca agacccgcgc cgaggtgaag ttcgagggcg
     2461 acaccctggt gaaccgcatc gagctgaagg gcatcgactt caaggaggac ggcaacatcc
     2521 tggggcacaa gctggagtac aactacaaca gccacaacgt ctatatcatg gccgacaagc
     2581 agaagaacgg catcaaggtg aacttcaaga tccgccacaa catcgaggac ggcagcgtgc
     2641 agctcgccga ccactaccag cagaacaccc ccatcggcga cggccccgtg ctgctgcccg
     2701 acaaccacta cctgagcacc cagtccgccc tgagcaaaga ccccaacgag aagcgcgatc
     2761 acatggtcct gctggagttc gtgaccgccg ccgggatcac tctcggcatg gacgagctgt
     2821 acaagcatga tgaattatga cacccagctt tcttgtacaa agtggtgata tcaatggtga
     2881 gcaagggcga ggagctgttc accggggtgg tgcccatcct ggtcgagctg gacggcgacg
     2941 taaacggcca caagttcagc gtgtccggcg agggcgaggg cgatgccacc tacggcaagc
     3001 tgaccctgaa gttcatctgc accaccggca agctgcccgt gccctggccc accctcgtga
     3061 ccaccctgac ctacggcgtg cagtgcttca gccgctaccc cgaccacatg aagcagcacg
     3121 acttcttcaa gtccgccatg cccgaaggct acgtccagga gcgcaccatc ttcttcaagg
     3181 acgacggcaa ctacaagacc cgcgccgagg tgaagttcga gggcgacacc ctggtgaacc
     3241 gcatcgagct gaagggcatc gacttcaagg aggacggcaa catcctgggg cacaagctgg
     3301 agtacaacta caacagccac aacgtctata tcatggccga caagcagaag aacggcatca
     3361 aggtgaactt caagatccgc cacaacatcg aggacggcag cgtgcagctc gccgaccact
     3421 accagcagaa cacccccatc ggcgacggcc ccgtgctgct gcccgacaac cactacctga
     3481 gcacccagtc cgccctgagc aaagacccca acgagaagcg cgatcacatg gtcctgctgg
     3541 agttcgtgac cgccgccggg atcactctcg gcatggacga gctgtacaag taaccgcggc
     3601 catgctagag tccgcaaaaa tcaccagtct ctctctacaa atctatctct ctctattttt
     3661 ctccagaata atgtgtgagt agttcccaga taagggaatt agggttctta tagggtttcg
     3721 ctcatgtgtt gagcatataa gaaaccctta gtatgtattt gtatttgtaa aatacttcta
     3781 tcaataaaat ttctaattcc taaaaccaaa atccagtgac ctgcaggcat gcgacgtcgg
     3841 gccctctaga ggatccccgg gtaccgcgaa ttatcgatca tgagcggaga attaagggag
     3901 tcacgttatg acccccgccg atgacgcggg acaagccgtt ttacgtttgg aactgacaga
     3961 accgcaacgt tgaaggagcc actgagccgc gggtttctgg agtttaatga gctaagcaca
     4021 tacgtcagaa accattattg cgcgttcaaa agtcgcctaa ggtcactatc agctagcaaa
     4081 tatttcttgt caaaaatgct ccactgacgt tccataaatt cccctcggta tccaattaga
     4141 gtctcatatt cactctcaac tcgatcgagg ggatctacca tgagcccaga acgacgcccg
     4201 gccgacatcc gccgtgccac cgaggcggac atgccggcgg tctgcaccat cgtcaaccac
     4261 tacatcgaga caagcacggt caacttccgt accgagccgc aggaaccgca ggagtggacg
     4321 gacgacctcg tccgtctgcg ggagcgctat ccctggctcg tcgccgaggt ggacggcgag
     4381 gtcgccggca tcgcctacgc gggtccctgg aaggcacgca acgcctacga ctggacggcc
     4441 gagtcgaccg tgtacgtctc cccccgccac cagcggacgg gactgggctc cacgctctac
     4501 acccacctgc tgaagtccct ggaggcacag ggcttcaaga gcgtggtcgc tgtcatcggg
     4561 ctgcccaacg acccgagcgt gcgcatgcac gaggcgctcg gatatgcccc ccgcggcatg
     4621 ctgcgggcgg ccggcttcaa gcacgggaac tggcatgacg tgggtttctg gcagctggac
     4681 ttcagcctgc cggtgccgcc ccgtccggtc ctgcccgtca ccgaaatctg atgaccccta
     4741 gagtcaagca gatcgttcaa acatttggca ataaagtttc ttaagattga atcctgttgc
     4801 cggtcttgcg atgattatca tataatttct gttgaattac gttaagcatg taataattaa
     4861 catgtaatgc atgacgttat ttatgagatg ggtttttatg attagagtcc cgcaattata
     4921 catttaatac gcgatagaaa acaaaatata gcgcgcaaac taggataaat tatcgcgcgc
     4981 ggtgtcatct atgttactag atcgaccggc atgcaagctg ataattcaat tcggcgttaa
     5041 ttcagtacat taaaaacgtc cgcaatgtgt tattaagttg tctaagcgtc aatttgttta
     5101 caccacaata tatcctgcca ccagccagcc aacagctccc cgaccggcag ctcggcacaa
     5161 aatcaccact cgatacaggc agcccatcag tccgggacgg cgtcagcggg agagccgttg
     5221 taaggcggca gactttgctc atgttaccga tgctattcgg aagaacggca actaagctgc
     5281 cgggtttgaa acacggatga tctcgcggag ggtagcatgt tgattgtaac gatgacagag
     5341 cgttgctgcc tgtgatcaat tcgggcacga acccagtgga cataagcctg ttcggttcgt
     5401 aagctgtaat gcaagtagcg tatgcgctca cgcaactggt ccagaacctt gaccgaacgc
     5461 agcggtggta acggcgcagt ggcggttttc atggcttgtt atgactgttt ttttggggta
     5521 cagtctatgc ctcgggcatc caagcagcaa gcgcgttacg ccgtgggtcg atgtttgatg
     5581 ttatggagca gcaacgatgt tacgcagcag ggcagtcgcc ctaaaacaaa gttaaacatc
     5641 atgggggaag cggtgatcgc cgaagtatcg actcaactat cagaggtagt tggcgtcatc
     5701 gagcgccatc tcgaaccgac gttgctggcc gtacatttgt acggctccgc agtggatggc
     5761 ggcctgaagc cacacagtga tattgatttg ctggttacgg tgaccgtaag gcttgatgaa
     5821 acaacgcggc gagctttgat caacgacctt ttggaaactt cggcttcccc tggagagagc
     5881 gagattctcc gcgctgtaga agtcaccatt gttgtgcacg acgacatcat tccgtggcgt
     5941 tatccagcta agcgcgaact gcaatttgga gaatggcagc gcaatgacat tcttgcaggt
     6001 atcttcgagc cagccacgat cgacattgat ctggctatct tgctgacaaa agcaagagaa
     6061 catagcgttg ccttggtagg tccagcggcg gaggaactct ttgatccggt tcctgaacag
     6121 gatctatttg aggcgctaaa tgaaacctta acgctatgga actcgccgcc cgactgggct
     6181 ggcgatgagc gaaatgtagt gcttacgttg tcccgcattt ggtacagcgc agtaaccggc
     6241 aaaatcgcgc cgaaggatgt cgctgccgac tgggcaatgg agcgcctgcc ggcccagtat
     6301 cagcccgtca tacttgaagc tagacaggct tatcttggac aagaagaaga tcgcttggcc
     6361 tcgcgcgcag atcagttgga agaatttgtc cactacgtga aaggcgagat caccaaggta
     6421 gtcggcaaat aatgtctagc tagaaattcg ttcaagccga cgccgcttcg cggcgcggct
     6481 taactcaagc gttagatgca ctaagcacat aattgctcac agccaaacta tcaggtcaag
     6541 tctgctttta ttatttttaa gcgtgcataa taagccctac acaaattggg agatatatca
     6601 tgcatgacca aaatccctta acgtgagttt tcgttccact gagcgtcaga ccccgtagaa
     6661 aagatcaaag gatcttcttg agatcctttt tttctgcgcg taatctgctg cttgcaaaca
     6721 aaaaaaccac cgctaccagc ggtggtttgt ttgccggatc aagagctacc aactcttttt
     6781 ccgaaggtaa ctggcttcag cagagcgcag ataccaaata ctgtccttct agtgtagccg
     6841 tagttaggcc accacttcaa gaactctgta gcaccgccta catacctcgc tctgctaatc
     6901 ctgttaccag tggctgctgc cagtggcgat aagtcgtgtc ttaccgggtt ggactcaaga
     6961 cgatagttac cggataaggc gcagcggtcg ggctgaacgg ggggttcgtg cacacagccc
     7021 agcttggagc gaacgaccta caccgaactg agatacctac agcgtgagct atgagaaagc
     7081 gccacgcttc ccgaagggag aaaggcggac aggtatccgg taagcggcag ggtcggaaca
     7141 ggagagcgca cgagggagct tccaggggga aacgcctggt atctttatag tcctgtcggg
     7201 tttcgccacc tctgacttga gcgtcgattt ttgtgatgct cgtcaggggg gcggagccta
     7261 tggaaaaacg ccagcaacgc ggccttttta cggttcctgg ccttttgctg gccttttgct
     7321 cacatgttct ttcctgcgtt atcccctgat tctgtggata accgtattac cgcctttgag
     7381 tgagctgata ccgctcgccg cagccgaacg accgagcgca gcgagtcagt gagcgaggaa
     7441 gcggaagagc gcctgatgcg gtattttctc cttacgcatc tgtgcggtat ttcacaccgc
     7501 atatggtgca ctctcagtac aatctgctct gatgccgcat agttaagcca gtatacactc
     7561 cgctatcgct acgtgactgg gtcatggctg cgccccgaca cccgccaaca cccgctgacg
     7621 cgccctgacg ggcttgtctg ctcccggcat ccgcttacag acaagctgtg accgtctccg
     7681 ggagctgcat gtgtcagagg ttttcaccgt catcaccgaa acgcgcgagg cagggtgcct
     7741 tgatgtgggc gccggcggtc gagtggcgac ggcgcggctt gtccgcgccc tggtagattg
     7801 cctggccgta ggccagccat ttttgagcgg ccagcggccg cgataggccg acgcgaagcg
     7861 gcggggcgta gggagcgcag cgaccgaagg gtaggcgctt tttgcagctc ttcggctgtg
     7921 cgctggccag acagttatgc acaggccagg cgggttttaa gagttttaat aagttttaaa
     7981 gagttttagg cggaaaaatc gccttttttc tcttttatat cagtcactta catgtgtgac
     8041 cggttcccaa tgtacggctt tgggttccca atgtacgggt tccggttccc aatgtacggc
     8101 tttgggttcc caatgtacgt gctatccaca ggaaagagac cttttcgacc tttttcccct
     8161 gctagggcaa tttgccctag catctgctcc gtacattagg aaccggcgga tgcttcgccc
     8221 tcgatcaggt tgcggtagcg catgactagg atcgggccag cctgccccgc ctcctccttc
     8281 aaatcgtact ccggcaggtc atttgacccg atcagcttgc gcacggtgaa acagaacttc
     8341 ttgaactctc cggcgctgcc actgcgttcg tagatcgtct tgaacaacca tctggcttct
     8401 gccttgcctg cggcgcggcg tgccaggcgg tagagaaaac ggccgatgcc gggatcgatc
     8461 aaaaagtaat cggggtgaac cgtcagcacg tccgggttct tgccttctgt gatctcgcgg
     8521 tacatccaat cagctagctc gatctcgatg tactccggcc gcccggtttc gctctttacg
     8581 atcttgtagc ggctaatcaa ggcttcaccc tcggataccg tcaccaggcg gccgttcttg
     8641 gccttcttcg tacgctgcat ggcaacgtgc gtggtgttta accgaatgca ggtttctacc
     8701 aggtcgtctt tctgctttcc gccatcggct cgccggcaga acttgagtac gtccgcaacg
     8761 tgtggacgga acacgcggcc gggcttgtct cccttccctt cccggtatcg gttcatggat
     8821 tcggttagat gggaaaccgc catcagtacc aggtcgtaat cccacacact ggccatgccg
     8881 gccggccctg cggaaacctc tacgtgcccg tctggaagct cgtagcggat cacctcgcca
     8941 gctcgtcggt cacgcttcga cagacggaaa acggccacgt ccatgatgct gcgactatcg
     9001 cgggtgccca cgtcatagag catcggaacg aaaaaatctg gttgctcgtc gcccttgggc
     9061 ggcttcctaa tcgacggcgc accggctgcc ggcggttgcc gggattcttt gcggattcga
     9121 tcagcggccg cttgccacga ttcaccgggg cgtgcttctg cctcgatgcg ttgccgctgg
     9181 gcggcctgcg cggccttcaa cttctccacc aggtcatcac ccagcgccgc gccgatttgt
     9241 accgggccgg atggtttgcg accgctcacg ccgattcctc gggcttgggg gttccagtgc
     9301 cattgcaggg ccggcagaca acccagccgc ttacgcctgg ccaaccgccc gttcctccac
     9361 acatggggca ttccacggcg tcggtgcctg gttgttcttg attttccatg ccgcctcctt
     9421 tagccgctaa aattcatcta ctcatttatt catttgctca tttactctgg tagctgcgcg
     9481 atgtattcag atagcagctc ggtaatggtc ttgccttggc gtaccgcgta catcttcagc
     9541 ttggtgtgat cctccgccgg caactgaaag ttgacccgct tcatggctgg cgtgtctgcc
     9601 aggctggcca acgttgcagc cttgctgctg cgtgcgctcg gacggccggc acttagcgtg
     9661 tttgtgcttt tgctcatttt ctctttacct cattaactca aatgagtttt gatttaattt
     9721 cagcggccag cgcctggacc tcgcgggcag cgtcgccctc gggttctgat tcaagaacgg
     9781 ttgtgccggc ggcggcagtg cctgggtagc tcacgcgctg cgtgatacgg gactcaagaa
     9841 tgggcagctc gtacccggcc agcgcctcgg caacctcacc gccgatgcgc gtgcctttga
     9901 tcgcccgcga cacgacaaag gccgcttgta gccttccatc cgtgacctca atgcgctgct
     9961 taaccagctc caccaggtcg gcggtggccc atatgtcgta agggcttggc tgcaccggaa
    10021 tcagcacgaa gtcggctgcc ttgatcgcgg acacagccaa gtccgccgcc tggggcgctc
    10081 cgtcgatcac tacgaagtcg cgccggccga tggccttcac gtcgcggtca atcgtcgggc
    10141 ggtcgatgcc gacaacggtt agcggttgat cttcccgcac ggccgcccaa tcgcgggcac
    10201 tgccctgggg atcggaatcg actaacagaa catcggcccc ggcgagttgc agggcgcggg
    10261 ctagatgggt tgcgatggtc gtcttgcctg acccgccttt ctggttaagt acagcgataa
    10321 ccttcatgcg ttccccttgc gtatttgttt atttactcat cgcatcatat acgcagcgac
    10381 cgcatgacgc aagctgtttt actcaaatac acatcacctt tttagacggc ggcgctcggt
    10441 ttcttcagcg gccaagctgg ccggccaggc cgccagcttg gcatcagaca aaccggccag
    10501 gatttcatgc agccgcacgg ttgagacgtg cgcgggcggc tcgaacacgt acccggccgc
    10561 gatcatctcc gcctcgatct cttcggtaat gaaaaacggt tcgtcctggc cgtcctggtg
    10621 cggtttcatg cttgttcctc ttggcgttca ttctcggcgg ccgccagggc gtcggcctcg
    10681 gtcaatgcgt cctcacggaa ggcaccgcgc cgcctggcct cggtgggcgt cacttcctcg
    10741 ctgcgctcaa gtgcgcggta cagggtcgag cgatgcacgc caagcagtgc agccgcctct
    10801 ttcacggtgc ggccttcctg gtcgatcagc tcgcgggcgt gcgcgatctg tgccggggtg
    10861 agggtagggc gggggccaaa cttcacgcct cgggccttgg cggcctcgcg cccgctccgg
    10921 gtgcggtcga tgattaggga acgctcgaac tcggcaatgc cggcgaacac ggtcaacacc
    10981 atgcggccgg ccggcgtggt ggtgtcggcc cacggctctg ccaggctacg caggcccgcg
    11041 ccggcctcct ggatgcgctc ggcaatgtcc agtaggtcgc gggtgctgcg ggccaggcgg
    11101 tctagcctgg tcactgtcac aacgtcgcca gggcgtaggt ggtcaagcat cctggccagc
    11161 tccgggcggt cgcgcctggt gccggtgatc ttctcggaaa acagcttggt gcagccggcc
    11221 gcgtgcagtt cggcccgttg gttggtcaag tcctggtcgt cggtgctgac gcgggcatag
    11281 cccagcaggc cagcggcggc gctcttgttc atggcgtaat gtctccggtt ctagtcgcaa
    11341 gtattctact ttatgcgact aaaacacgcg acaagaaaac gccaggaaaa gggcagggcg
    11401 gcagcctgtc gcgtaactta ggacttgtgc gacatgtcgt tttcagaaga cggctgcact
    11461 gaacgtcaga agccgactgc actatagcag cggaggggtt ggatcaaagt actttaaagt
    11521 actttaaagt actttaaagt actttgatcc cgaggggaac cctgtggttg gcatgcacat
    11581 acaaatggac gaacggataa accttttcac gcccttttaa atatccgatt attctaataa
    11641 acgctctttt ctcttaggtt tacccgccaa tatatcctgt caaacactga tagtttaaac
    11701 tgaaggcggg aaacgacaat ctgatccaag ctcaagctaa gcttgagctc tcccatatgg
    11761 tcgactagag ccaagctgat ctcctttgcc ccggagatca ccatggacga ctttctctat
    11821 ctctacgatc taggaagaaa gttcgacgga gaaggtgacg ataccatgtt caccaccgat
    11881 aatgagaaga ttagcctctt caatttcaga aagaatgctg acccacagat ggttagagag
    11941 gcctacgcgg caggtctcat caagacgatc tacccgagta ataatctcca ggagatcaaa
    12001 taccttccca agaaggttaa agatgcagtc aaaagattca ggactaactg catcaagaac
    12061 acagagaaag atatatttct caagatcaga agtactattc cagtatggac gattcaaggc
    12121 ttgcttcata aaccaaggca agtaatagag attggagtct ctaagaaagt agttcctact
    12181 gaatcaaagg ccatggagtc aaaaattcag atcgaggatc taacagaact cgccgtgaag
    12241 actggcgaac agttcataca gagtctttta cgactcaatg acaagaagaa aatcttcgtc
    12301 aacatggtgg agcacgacac tctcgtctac tccaagaata tcaaagatac agtctcagaa
    12361 gaccaaaggg ctattgagac ttttcaacaa agggtaatat cgggaaacct cctcggattc
    12421 cattgcccag ctatctgtca cttcatcaaa aggacagtag aaaaggaagg tggcacctac
    12481 aaatgccatc attgcgataa aggaaaggct atcgttcaag atgcccctgc cgacagtggt
    12541 cccaaagatg gacccccacc cacgaggagc atcgtggaaa aagaagacgt tccaaccacg
    12601 tcttcaaagc aagtggattg atgtgatatc tccactgacg taagggatga cgcacaatcc
    12661 cactatcctt cgcaagaccc ttcctctata taaggaagtt catttcattt ggagaggact
    12721 ccggtatttt tacaacaatt accacaacaa aacaaacaac aaacaacatt acaatttact
    12781 attctagtcg acctgcaggc ggccgcacta gtgatatcac aagtttgtac aaaaaagcag
    12841 gctacaatga aagccttcac actcgctctc ttcttagctc tttccctcta tctcctgccc
    12901 aatccagcca tggtgagcaa gggcgaggag gataacatgg ccatcatcaa ggagttcatg
    12961 cgtttcaagg tgcacatgga gggctccgtg aacggccacg agttcgagat cgagggcgag
    13021 ggcgagggcc gcccctacga gggcacccag accgccaagc tgaaggtgac caagggtggc
    13081 cccctgccct tcgcctggga catcctgtcc cctcagttca tgtacggctc caaggcctac
    13141 gtgaagcacc ccgccgacat ccccgactac ttgaagctgt ccttccccga gggcttcaag
    13201 tgggagcgcg tgatgaactt cgaggacggc ggcgtggtga ccgtgaccca ggactcctcc
    13261 ctgcaggacg gcgagttcat ctacaaggtg aagctgcgcg gcaccaactt cccctccgac
    13321 ggccccgtaa tgcagaagaa gaccatgggc tgggaggcct cctccgagcg gatgtacccc
    13381 gaggacggcg ccctgaaggg cgagatcaag cagaggctga agctgaagga cggcggccac
    13441 tacgacgctg aggtcaagac cacctacaag gccaagaagc ccgtgcagct gcccggcgcc
    13501 tacaacgtca acatcaagtt ggacatcacc tcccacaacg aggactacac catcgtggaa
    13561 cagtacgaac gcgccgaggg ccgccactcc accggcggca tggacgagct gtacaagggt
    13621 ggcagtggag gtatggtgag caagggcgag gagctgttca ccggggtggt gcccatcctg
    13681 gtcgagctgg acggcgacgt aaacggccac aagttcagcg tgtccggcga gggcgagggc
    13741 gatgccacct acggcaagct gaccctgaag ttcatctgca ccaccggcaa gctgcccgtg
    13801 ccctggccca ccctcgtgac caccctgacc tacggcgtgc agtgcttcag ccgctacccc
    13861 gaccacatga agcagcacga cttcttcaag tccgccatgc ccgaaggcta cgtccaggag
    13921 cgcaccatct tcttcaagga cgacggcaac tacaagaccc gcgccgaggt gaagttcgag
    13981 ggcgacaccc tggtgaaccg catcgagctg aagggcatcg acttcaagga ggacggcaac
    14041 atcctggggc acaagctgga gtacaactac aacagccaca acgtctatat catggccgac
    14101 aagcagaaga acggcatcaa ggtgaacttc aagatccgcc acaacatcga ggacggcagc
    14161 gtgcagctcg ccgaccacta ccagcagaac acccccatcg gcgacggccc cgtgctgctg
    14221 cccgacaacc actacctgag cacccagtcc gccctgagca aagaccccaa cgagaagcgc
    14281 gatcacatgg tcctgctgga gttcgtgacc gccgccggga tcactctcgg catggacgag
    14341 ctgtacaagc atgatgaatt atgacaccca gctttcttgt acaaagttgg cattataaga
    14401 aagcattgct tatcaatttg ttgcaacgaa caggtcacta tcagtcaaaa taaaatcatt
    14461 atttgccatc cagctgatat cccctatagt gagtcgtatt acatggtcat agctgtttcc
    14521 tggcagctct ggcccgtgtc tcaaaatctc tgatgttaca ttgcacaaga taaaaatata
    14581 tcatcatgat cagtcctgct cctcggccac gaagtgcacg cagttgccgg ccgggtcgcg
    14641 cagggcgaac tcccgccccc acggctgctc gccgatctcg gtcatggccg gcccggaggc
    14701 gtcccggaag ttcgtggaca cgacctccga ccactcggcg tacagctcgt ccaggccgcg
    14761 cacccacacc caggccaggg tgttgtccgg caccacctgg tcctggaccg cgctgatgaa
    14821 cagggtcacg tcgtcccgga ccacaccggc gaagtcgtcc tccacgaagt cccgggagaa
    14881 cccgagccgg tcggtccaga actcgaccgc tccggcgacg tcgcgcgcgg tgagcaccgg
    14941 aacggcactg gtcaacttgg ccatggttta gttcctcacc ttgtcgtatt atactatgcc
    15001 gatatactat gccgatgatt aattgtcaac acgtgctgat catgaccaaa atcccttaac
    15061 gtgagttacg cgtcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttc
//
