LOCUS       sequence-411022-        5968 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  SDCBP protein expression.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5968)
  TITLE     TREAT-AD plasmids Jun 2023
REFERENCE   2  (bases 1 to 5968)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: SDCBPA-c012
FEATURES             Location/Qualifiers
     source          1..5968
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     128..145
                     /label=L4440
                     /note="L4440 vector, forward primer"
     misc_feature    160..302
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     complement(228..247)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     388..410
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     CDS             complement(404..595)
                     /codon_start=1
                     /gene="rop"
                     /product="Rop protein, which maintains plasmids at low copy
                     number"
                     /label=rop
                     /translation="MTKQEKTALNMARFIRSQTLTLLEKLNELDADEQADICESLHDHA
                     DELYRSCLARFGDDGENL"
     CDS             complement(1404..2486)
                     /codon_start=1
                     /gene="lacI"
                     /product="lac repressor"
                     /label=lacI
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
                     /translation="MKPVTLYDVAEYAGVSYQTVSRVVNQASHVSAKTREKVEAAMAEL
                     NYIPNRVAQQLAGKQSLLIGVATSSLALHAPSQIVAAIKSRADQLGASVVVSMVERSGV
                     EACKAAVHNLLAQRVSGLIINYPLDDQDAIAVEAACTNVPALFLDVSDQTPINSIIFSH
                     EDGTRLGVEHLVALGHQQIALLAGPLSSVSARLRLAGWHKYLTRNQIQPIAEREGDWSA
                     MSGFQQTMQMLNEGIVPTAMLVANDQMALGAMRAITESGLRVGADISVVGYDDTEDSSC
                     YIPPLTTIKQDFRLLGQTSVDRLLQLSQGQAVKGNQLLPVSLVKRKTTLAPNTQTASPR
                     ALADSLMQLARQVSRLESGQ"
     primer_bind     2448..2467
                     /label=LacI-R
                     /note="LacI, reverse primer"
     promoter        complement(2487..2564)
                     /gene="lacI"
                     /label=lacI promoter
     primer_bind     2770..2789
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
     primer_bind     2873..2892
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        2873..2891
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     protein_bind    2892..2916
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     RBS             2931..2953
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             2966..2983
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             3008..3028
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQS"
     CDS             3629..3673
                     /codon_start=1
                     /product="peptide tag that allows for enzymatic 
                     biotinylation"
                     /label=AviTag(TM)
                     /translation="GLNDIFEAQKIEWHE"
     CDS             3701..3718
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(3771..3789)
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
     terminator      3785..3832
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     rep_origin      3869..4324
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(3956..3975)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     4166..4187
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     CDS             complement(4417..5232)
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     CDS             complement(4417..5232)
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     5140..5159
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     primer_bind     5224..5243
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     rep_origin      5354..5942
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     5843..5862
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 gttcctggcc ttttgctggc cttttgctca catgttcttt cctgcgttat cccctgattc
       61 tgtggataac cgtattaccg cctttgagtg agctgatacc gctcgccgca gccgaacgac
      121 cgagcgcagc gagtcagtga gcgaggaagc ggaagagcgc ctgatgcggt attttctcct
      181 tacgcatctg tgcggtattt cacaccgcat atatggtgca ctctcagtac aatctgctct
      241 gatgccgcat agttaagcca gtatacactc cgctatcgct acgtgactgg gtcatggctg
      301 cgccccgaca cccgccaaca cccgctgacg cgccctgacg ggcttgtctg ctcccggcat
      361 ccgcttacag acaagctgtg accgtctccg ggagctgcat gtgtcagagg ttttcaccgt
      421 catcaccgaa acgcgcgagg cagctgcggt aaagctcatc agcgtggtcg tgaagcgatt
      481 cacagatgtc tgcctgttca tccgcgtcca gctcgttgag tttctccaga agcgttaatg
      541 tctggcttct gataaagcgg gccatgttaa gggcggtttt ttcctgtttg gtcactgatg
      601 cctccgtgta agggggattt ctgttcatgg gggtaatgat accgatgaaa cgagagagga
      661 tgctcacgat acgggttact gatgatgaac atgcccggtt actggaacgt tgtgagggta
      721 aacaactggc ggtatggatg cggcgggacc agagaaaaat cactcagggt caatgccagc
      781 gcttcgttaa tacagatgta ggtgttccac agggtagcca gcagcatcct gcgatgcaga
      841 tccggaacat aatggtgcag ggcgctgact tccgcgtttc cagactttac gaaacacgga
      901 aaccgaagac cattcatgtt gttgctcagg tcgcagacgt tttgcagcag cagtcgcttc
      961 acgttcgctc gcgtatcggt gattcattct gctaaccagt aaggcaaccc cgccagccta
     1021 gccgggtcct caacgacagg agcacgatca tgcgcacccg tggggccgcc atgccggcga
     1081 taatggcctg cttctcgccg aaacgtttgg tggcgggacc agtgacgaag gcttgagcga
     1141 gggcgtgcaa gattccgaat accgcaagcg acaggccgat catcgtcgcg ctccagcgaa
     1201 agcggtcctc gccgaaaatg acccagagcg ctgccggcac ctgtcctacg agttgcatga
     1261 taaagaagac agtcataagt gcggcgacga tagtcatgcc ccgcgcccac cggaaggagc
     1321 tgactgggtt gaaggctctc aagggcatcg gtcgagatcc cggtgcctaa tgagtgagct
     1381 aacttacatt aattgcgttg cgctcactgc ccgctttcca gtcgggaaac ctgtcgtgcc
     1441 agctgcatta atgaatcggc caacgcgcgg ggagaggcgg tttgcgtatt gggcgccagg
     1501 gtggtttttc ttttcaccag tgagacgggc aacagctgat tgcccttcac cgcctggccc
     1561 tgagagagtt gcagcaagcg gtccacgctg gtttgcccca gcaggcgaaa atcctgtttg
     1621 atggtggtta acggcgggat ataacatgag ctgtcttcgg tatcgtcgta tcccactacc
     1681 gagatatccg caccaacgcg cagcccggac tcggtaatgg cgcgcattgc gcccagcgcc
     1741 atctgatcgt tggcaaccag catcgcagtg ggaacgatgc cctcattcag catttgcatg
     1801 gtttgttgaa aaccggacat ggcactccag tcgccttccc gttccgctat cggctgaatt
     1861 tgattgcgag tgagatattt atgccagcca gccagacgca gacgcgccga gacagaactt
     1921 aatgggcccg ctaacagcgc gatttgctgg tgacccaatg cgaccagatg ctccacgccc
     1981 agtcgcgtac cgtcttcatg ggagaaaata atactgttga tgggtgtctg gtcagagaca
     2041 tcaagaaata acgccggaac attagtgcag gcagcttcca cagcaatggc atcctggtca
     2101 tccagcggat agttaatgat cagcccactg acgcgttgcg cgagaagatt gtgcaccgcc
     2161 gctttacagg cttcgacgcc gcttcgttct accatcgaca ccaccacgct ggcacccagt
     2221 tgatcggcgc gagatttaat cgccgcgaca atttgcgacg gcgcgtgcag ggccagactg
     2281 gaggtggcaa cgccaatcag caacgactgt ttgcccgcca gttgttgtgc cacgcggttg
     2341 ggaatgtaat tcagctccgc catcgccgct tccacttttt cccgcgtttt cgcagaaacg
     2401 tggctggcct ggttcaccac gcgggaaacg gtctgataag agacaccggc atactctgcg
     2461 acatcgtata acgttactgg tttcacattc accaccctga attgactctc ttccgggcgc
     2521 tatcatgcca taccgcgaaa ggttttgcgc cattcgatgg tgtccgggat ctcgacgctc
     2581 tcccttatgc gactcctgca ttaggaagca gcccagtagt aggttgaggc cgttgagcac
     2641 cgccgccgca aggaatggtg catgcaagga gatggcgccc aacagtcccc cggccacggg
     2701 gcctgccacc atacccacgc cgaaacaagc gctcatgagc ccgaagtggc gagcccgatc
     2761 ttccccatcg gtgatgtcgg cgatataggc gccagcaacc gcacctgtgg cgccggtgat
     2821 gccggccacg atgcgtccgg cgtagaggat cgagatctcg atcccgcgaa attaatacga
     2881 ctcactatag gggaattgtg agcggataac aattcccctc tagaaataat tttgtttaac
     2941 tttaagaagg agatatacca tgggacacca tcatcatcat cattcttctg gtgtagatct
     3001 gggtaccgag aacctgtact tccaatccat ggcagaaatt aagcaaggga ttcgtgaagt
     3061 cattttgtgt aaggatcaag atggaaaaat tggactcagg cttaaatcaa tagataatgg
     3121 tatatttgtt cagctagtcc aggctaattc tccagcctca ttggttggtc tgagatttgg
     3181 ggaccaagta cttcagatca atggtgaaaa ctgtgcagga tggagctctg ataaagcgca
     3241 caaggtgctc aaacaggctt ttggagagaa gattaccatg accattcgtg acaggccctt
     3301 tgaacggacg attaccatgc ataaggatag cactggacat gttggtttta tctttaaaaa
     3361 tggaaaaata acatccatag tgaaagatag ctctgcagcc agaaatggtc ttctcacgga
     3421 acataacatc tgtgaaatca atggacagaa tgtcattgga ttgaaggact ctcaaattgc
     3481 agacatactg tcaacatctg ggactgtagt tactattaca atcatgcctg cttttatctt
     3541 tgaacatatt attaagcgga tggcaccaag cattatgaaa agcctaatgg accacaccat
     3601 tcctgaggtt ggaagtaaag gtggatacgg cctgaatgat atctttgaag cgcagaagat
     3661 tgaatggcat gaatgataaa agcttgcggc cgcactcgag caccaccacc accaccactg
     3721 agatccggct gctaacaaag cccgaaagga agctgagttg gctgctgcca ccgctgagca
     3781 ataactagca taaccccttg gggcctctaa acgggtcttg aggggttttt tgctgaaagg
     3841 aggaactata tccggattgg cgaatgggac gcgccctgta gcggcgcatt aagcgcggcg
     3901 ggtgtggtgg ttacgcgcag cgtgaccgct acacttgcca gcgccctagc gcccgctcct
     3961 ttcgctttct tcccttcctt tctcgccacg ttcgccggct ttccccgtca agctctaaat
     4021 cgggggctcc ctttagggtt ccgatttagt gctttacggc acctcgaccc caaaaaactt
     4081 gattagggtg atggttcacg tagtgggcca tcgccctgat agacggtttt tcgccctttg
     4141 acgttggagt ccacgttctt taatagtgga ctcttgttcc aaactggaac aacactcaac
     4201 cctatctcgg tctattcttt tgatttataa gggattttgc cgatttcggc ctattggtta
     4261 aaaaatgagc tgatttaaca aaaatttaac gcgaatttta acaaaatatt aacgtttaca
     4321 atttcaggtg gcacttttcg gggaaatgtg cgcggaaccc ctatttgttt atttttctaa
     4381 atacattcaa atatgtatcc gctcatgaat taattcttag aaaaactcat cgagcatcaa
     4441 atgaaactgc aatttattca tatcaggatt atcaatacca tatttttgaa aaagccgttt
     4501 ctgtaatgaa ggagaaaact caccgaggca gttccatagg atggcaagat cctggtatcg
     4561 gtctgcgatt ccgactcgtc caacatcaat acaacctatt aatttcccct cgtcaaaaat
     4621 aaggttatca agtgagaaat caccatgagt gacgactgaa tccggtgaga atggcaaaag
     4681 tttatgcatt tctttccaga cttgttcaac aggccagcca ttacgctcgt catcaaaatc
     4741 actcgcatca accaaaccgt tattcattcg tgattgcgcc tgagcgagac gaaatacgcg
     4801 atcgctgtta aaaggacaat tacaaacagg aatcgaatgc aaccggcgca ggaacactgc
     4861 cagcgcatca acaatatttt cacctgaatc aggatattct tctaatacct ggaatgctgt
     4921 tttcccgggg atcgcagtgg tgagtaacca tgcatcatca ggagtacgga taaaatgctt
     4981 gatggtcgga agaggcataa attccgtcag ccagtttagt ctgaccatct catctgtaac
     5041 atcattggca acgctacctt tgccatgttt cagaaacaac tctggcgcat cgggcttccc
     5101 atacaatcga tagattgtcg cacctgattg cccgacatta tcgcgagccc atttataccc
     5161 atataaatca gcatccatgt tggaatttaa tcgcggccta gagcaagacg tttcccgttg
     5221 aatatggctc ataacacccc ttgtattact gtttatgtaa gcagacagtt ttattgttca
     5281 tgaccaaaat cccttaacgt gagttttcgt tccactgagc gtcagacccc gtagaaaaga
     5341 tcaaaggatc ttcttgagat cctttttttc tgcgcgtaat ctgctgcttg caaacaaaaa
     5401 aaccaccgct accagcggtg gtttgtttgc cggatcaaga gctaccaact ctttttccga
     5461 aggtaactgg cttcagcaga gcgcagatac caaatactgt ccttctagtg tagccgtagt
     5521 taggccacca cttcaagaac tctgtagcac cgcctacata cctcgctctg ctaatcctgt
     5581 taccagtggc tgctgccagt ggcgataagt cgtgtcttac cgggttggac tcaagacgat
     5641 agttaccgga taaggcgcag cggtcgggct gaacgggggg ttcgtgcaca cagcccagct
     5701 tggagcgaac gacctacacc gaactgagat acctacagcg tgagctatga gaaagcgcca
     5761 cgcttcccga agggagaaag gcggacaggt atccggtaag cggcagggtc ggaacaggag
     5821 agcgcacgag ggagcttcca gggggaaacg cctggtatct ttatagtcct gtcgggtttc
     5881 gccacctctg acttgagcgt cgatttttgt gatgctcgtc aggggggcgg agcctatgga
     5941 aaaacgccag caacgcggcc tttttacg
//
