LOCUS       sequence-411056-        5920 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  SDCBP protein expression.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5920)
  TITLE     TREAT-AD plasmids Jun 2023
REFERENCE   2  (bases 1 to 5920)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: SDCBPA-c023
FEATURES             Location/Qualifiers
     source          1..5920
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     671..690
                     /label=LacI-R
                     /note="LacI, reverse primer"
     promoter        complement(710..787)
                     /gene="lacI"
                     /label=lacI promoter
     primer_bind     993..1012
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
     primer_bind     1096..1115
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        1096..1114
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     protein_bind    1115..1139
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     CDS             1768..1788
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQS"
     CDS             1789..1806
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             1816..1830
                     /codon_start=1
                     /product="enterokinase recognition and cleavage site"
                     /label=enterokinase site
                     /translation="DDDDK"
     CDS             1880..1897
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(1950..1968)
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
     terminator      1964..2011
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     rep_origin      2048..2503
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(2135..2154)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     2345..2366
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     CDS             complement(2595..3410)
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     CDS             complement(2595..3410)
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     3318..3337
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     primer_bind     3402..3421
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     rep_origin      3532..4120
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     4021..4040
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     4274..4291
                     /label=L4440
                     /note="L4440 vector, forward primer"
     misc_feature    4306..4445
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     complement(4371..4390)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     4531..4553
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     CDS             complement(4547..4738)
                     /codon_start=1
                     /gene="rop"
                     /product="Rop protein, which maintains plasmids at low copy
                     number"
                     /label=rop
                     /translation="MTKQEKTALNMARFIRSQTLTLLEKLNELDADEQADICESLHDHA
                     DELYRSCLARFGDDGENL"
     CDS             complement(join(5547..5920,1..709))
                     /codon_start=1
                     /gene="lacI"
                     /product="lac repressor"
                     /label=lacI
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
                     /translation="MKPVTLYDVAEYAGVSYQTVSRVVNQASHVSAKTREKVEAAMAEL
                     NYIPNRVAQQLAGKQSLLIGVATSSLALHAPSQIVAAIKSRADQLGASVVVSMVERSGV
                     EACKAAVHNLLAQRVSGLIINYPLDDQDAIAVEAACTNVPALFLDVSDQTPINSIIFSH
                     EDGTRLGVEHLVALGHQQIALLAGPLSSVSARLRLAGWHKYLTRNQIQPIAEREGDWSA
                     MSGFQQTMQMLNEGIVPTAMLVANDQMALGAMRAITESGLRVGADISVVGYDDTEDSSC
                     YIPPLTTIKQDFRLLGQTSVDRLLQLSQGQAVKGNQLLPVSLVKRKTTLAPNTQTASPR
                     ALADSLMQLARQVSRLESGQ"
ORIGIN
        1 tgccctcatt cagcatttgc atggtttgtt gaaaaccgga catggcactc cagtcgcctt
       61 cccgttccgc tatcggctga atttgattgc gagtgagata tttatgccag ccagccagac
      121 gcagacgcgc cgagacagaa cttaatgggc ccgctaacag cgcgatttgc tggtgaccca
      181 atgcgaccag atgctccacg cccagtcgcg taccgtcttc atgggagaaa ataatactgt
      241 tgatgggtgt ctggtcagag acatcaagaa ataacgccgg aacattagtg caggcagctt
      301 ccacagcaat ggcatcctgg tcatccagcg gatagttaat gatcagccca ctgacgcgtt
      361 gcgcgagaag attgtgcacc gccgctttac aggcttcgac gccgcttcgt tctaccatcg
      421 acaccaccac gctggcaccc agttgatcgg cgcgagattt aatcgccgcg acaatttgcg
      481 acggcgcgtg cagggccaga ctggaggtgg caacgccaat cagcaacgac tgtttgcccg
      541 ccagttgttg tgccacgcgg ttgggaatgt aattcagctc cgccatcgcc gcttccactt
      601 tttcccgcgt tttcgcagaa acgtggctgg cctggttcac cacgcgggaa acggtctgat
      661 aagagacacc ggcatactct gcgacatcgt ataacgttac tggtttcaca ttcaccaccc
      721 tgaattgact ctcttccggg cgctatcatg ccataccgcg aaaggttttg cgccattcga
      781 tggtgtccgg gatctcgacg ctctccctta tgcgactcct gcattaggaa gcagcccagt
      841 agtaggttga ggccgttgag caccgccgcc gcaaggaatg gtgcatgcaa ggagatggcg
      901 cccaacagtc ccccggccac ggggcctgcc accataccca cgccgaaaca agcgctcatg
      961 agcccgaagt ggcgagcccg atcttcccca tcggtgatgt cggcgatata ggcgccagca
     1021 accgcacctg tggcgccggt gatgccggcc acgatgcgtc cggcgtagag gatcgagatc
     1081 tcgatcccgc gaaattaata cgactcacta taggggaatt gtgagcggat aacaattccc
     1141 ctctagaaat aattttgttt aaccttaaga aggagatata ctatggctga aattaaacaa
     1201 ggaataaggg aggtcatcct ttgcaaggac caggatggta agatcggctt gcgcctgaag
     1261 tccatcgaca acggtatctt cgtgcaactg gttcaggcta atagcccagc tagcctggtg
     1321 ggtctgcgtt ttggtgatca agtgctgcag atcaacggcg agaactgcgc cggttggagc
     1381 tctgacaaag cacataaagt tttgaagcag gcgttcggcg aaaaaatcac gatgaccatc
     1441 cgtgatcgtc cgtttgagcg caccatcacc atgcacaagg actccactgg tcatgttggt
     1501 tttatcttta aaaacggcaa gatcacctcg atcgtgaaag acagctccgc ggcgcgtaat
     1561 ggcctgttga ccgagcacaa tatttgtgaa attaacggcc aaaacgtgat tggcctcaag
     1621 gacagccaga ttgcggatat tctgagcacc agtggtacag ttgttaccat tacgatcatg
     1681 ccggcgttca tcttcgagca cattattaaa agaatggcac cgagcatcat gaaatctctg
     1741 atggatcata ccattccgga agtcgcagag aacctctact tccaatcgca ccatcatcac
     1801 caccattgat acaaggatga cgacgataag tgaggatccg aattcgagct ccgtcgacaa
     1861 gcttgcggcc gcactcgagc accaccacca ccaccactga gatccggctg ctaacaaagc
     1921 ccgaaaggaa gctgagttgg ctgctgccac cgctgagcaa taactagcat aaccccttgg
     1981 ggcctctaaa cgggtcttga ggggtttttt gctgaaagga ggaactatat ccggattggc
     2041 gaatgggacg cgccctgtag cggcgcatta agcgcggcgg gtgtggtggt tacgcgcagc
     2101 gtgaccgcta cacttgccag cgccctagcg cccgctcctt tcgctttctt cccttccttt
     2161 ctcgccacgt tcgccggctt tccccgtcaa gctctaaatc gggggctccc tttagggttc
     2221 cgatttagtg ctttacggca cctcgacccc aaaaaacttg attagggtga tggttcacgt
     2281 agtgggccat cgccctgata gacggttttt cgccctttga cgttggagtc cacgttcttt
     2341 aatagtggac tcttgttcca aactggaaca acactcaacc ctatctcggt ctattctttt
     2401 gatttataag ggattttgcc gatttcggcc tattggttaa aaaatgagct gatttaacaa
     2461 aaatttaacg cgaattttaa caaaatatta acgcttacaa tttaggtggc acttttcggg
     2521 gaaatgtgcg cggaacccct atttgtttat ttttctaaat acattcaaat atgtatccgc
     2581 tcatgaatta attcttagaa aaactcatcg agcatcaaat gaaactgcaa tttattcata
     2641 tcaggattat caataccata tttttgaaaa agccgtttct gtaatgaagg agaaaactca
     2701 ccgaggcagt tccataggat ggcaagatcc tggtatcggt ctgcgattcc gactcgtcca
     2761 acatcaatac aacctattaa tttcccctcg tcaaaaataa ggttatcaag tgagaaatca
     2821 ccatgagtga cgactgaatc cggtgagaat ggcaaaagtt tatgcatttc tttccagact
     2881 tgttcaacag gccagccatt acgctcgtca tcaaaatcac tcgcatcaac caaaccgtta
     2941 ttcattcgtg attgcgcctg agcgagacga aatacgcgat cgctgttaaa aggacaatta
     3001 caaacaggaa tcgaatgcaa ccggcgcagg aacactgcca gcgcatcaac aatattttca
     3061 cctgaatcag gatattcttc taatacctgg aatgctgttt tcccggggat cgcagtggtg
     3121 agtaaccatg catcatcagg agtacggata aaatgcttga tggtcggaag aggcataaat
     3181 tccgtcagcc agtttagtct gaccatctca tctgtaacat cattggcaac gctacctttg
     3241 ccatgtttca gaaacaactc tggcgcatcg ggcttcccat acaatcgata gattgtcgca
     3301 cctgattgcc cgacattatc gcgagcccat ttatacccat ataaatcagc atccatgttg
     3361 gaatttaatc gcggcctaga gcaagacgtt tcccgttgaa tatggctcat aacacccctt
     3421 gtattactgt ttatgtaagc agacagtttt attgttcatg accaaaatcc cttaacgtga
     3481 gttttcgttc cactgagcgt cagaccccgt agaaaagatc aaaggatctt cttgagatcc
     3541 tttttttctg cgcgtaatct gctgcttgca aacaaaaaaa ccaccgctac cagcggtggt
     3601 ttgtttgccg gatcaagagc taccaactct ttttccgaag gtaactggct tcagcagagc
     3661 gcagatacca aatactgtcc ttctagtgta gccgtagtta ggccaccact tcaagaactc
     3721 tgtagcaccg cctacatacc tcgctctgct aatcctgtta ccagtggctg ctgccagtgg
     3781 cgataagtcg tgtcttaccg ggttggactc aagacgatag ttaccggata aggcgcagcg
     3841 gtcgggctga acggggggtt cgtgcacaca gcccagcttg gagcgaacga cctacaccga
     3901 actgagatac ctacagcgtg agctatgaga aagcgccacg cttcccgaag ggagaaaggc
     3961 ggacaggtat ccggtaagcg gcagggtcgg aacaggagag cgcacgaggg agcttccagg
     4021 gggaaacgcc tggtatcttt atagtcctgt cgggtttcgc cacctctgac ttgagcgtcg
     4081 atttttgtga tgctcgtcag gggggcggag cctatggaaa aacgccagca acgcggcctt
     4141 tttacggttc ctggcctttt gctggccttt tgctcacatg ttctttcctg cgttatcccc
     4201 tgattctgtg gataaccgta ttaccgcctt tgagtgagct gataccgctc gccgcagccg
     4261 aacgaccgag cgcagcgagt cagtgagcga ggaagcggaa gagcgcctga tgcggtattt
     4321 tctccttacg catctgtgcg gtatttcaca ccgcaatggt gcactctcag tacaatctgc
     4381 tctgatgccg catagttaag ccagtataca ctccgctatc gctacgtgac tgggtcatgg
     4441 ctgcgccccg acacccgcca acacccgctg acgcgccctg acgggcttgt ctgctcccgg
     4501 catccgctta cagacaagct gtgaccgtct ccgggagctg catgtgtcag aggttttcac
     4561 cgtcatcacc gaaacgcgcg aggcagctgc ggtaaagctc atcagcgtgg tcgtgaagcg
     4621 attcacagat gtctgcctgt tcatccgcgt ccagctcgtt gagtttctcc agaagcgtta
     4681 atgtctggct tctgataaag cgggccatgt taagggcggt tttttcctgt ttggtcactg
     4741 atgcctccgt gtaaggggga tttctgttca tgggggtaat gataccgatg aaacgagaga
     4801 ggatgctcac gatacgggtt actgatgatg aacatgcccg gttactggaa cgttgtgagg
     4861 gtaaacaact ggcggtatgg atgcggcggg accagagaaa aatcactcag ggtcaatgcc
     4921 agcgcttcgt taatacagat gtaggtgttc cacagggtag ccagcagcat cctgcgatgc
     4981 agatccggaa cataatggtg cagggcgctg acttccgcgt ttccagactt tacgaaacac
     5041 ggaaaccgaa gaccattcat gttgttgctc aggtcgcaga cgttttgcag cagcagtcgc
     5101 ttcacgttcg ctcgcgtatc ggtgattcat tctgctaacc agtaaggcaa ccccgccagc
     5161 ctagccgggt cctcaacgac aggagcacga tcatgcgcac ccgtggggcc gccatgccgg
     5221 cgataatggc ctgcttctcg ccgaaacgtt tggtggcggg accagtgacg aaggcttgag
     5281 cgagggcgtg caagattccg aataccgcaa gcgacaggcc gatcatcgtc gcgctccagc
     5341 gaaagcggtc ctcgccgaaa atgacccaga gcgctgccgg cacctgtcct acgagttgca
     5401 tgataaagaa gacagtcata agtgcggcga cgatagtcat gccccgcgcc caccggaagg
     5461 agctgactgg gttgaaggct ctcaagggca tcggtcgaga tcccggtgcc taatgagtga
     5521 gctaacttac attaattgcg ttgcgctcac tgcccgcttt ccagtcggga aacctgtcgt
     5581 gccagctgca ttaatgaatc ggccaacgcg cggggagagg cggtttgcgt attgggcgcc
     5641 agggtggttt ttcttttcac cagtgagacg ggcaacagct gattgccctt caccgcctgg
     5701 ccctgagaga gttgcagcaa gcggtccacg ctggtttgcc ccagcaggcg aaaatcctgt
     5761 ttgatggtgg ttaacggcgg gatataacat gagctgtctt cggtatcgtc gtatcccact
     5821 accgagatat ccgcaccaac gcgcagcccg gactcggtaa tggcgcgcat tgcgcccagc
     5881 gccatctgat cgttggcaac cagcatcgca gtgggaacga
//
