LOCUS       sequence-411091-         777 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Lentiviral expression vector encoding FIP200 mutant-mCherry.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 777)
  AUTHORS   Chen M, Ren X, Nguyen TN, Khuu G, Cook ASI, Lazarou M, Hurley JH
  TITLE     Structure and activation of the human autophagy-initiating 
            ULK1C:PI3KC3-C1 supercomplex
  JOURNAL   bioRxiv 2023.06.01.543278
REFERENCE   2  (bases 1 to 777)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "bioRxiv 
            2023.06.01.543278"
COMMENT     Sequence Label: pCDH1-CMV-FIP200-L324D/R543D/R548D-mCherry-Hygro
FEATURES             Location/Qualifiers
     source          1..777
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     misc_feature    1..53
                     /label=MCS
                     /note="multiple cloning site"
     regulatory      61..70
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             67..777
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /label=mCherry
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     primer_bind     complement(212..230)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     480..499
                     /label=mCherry-F
                     /note="mCherry, forward primer"
ORIGIN
        1 ctcgagctca agcttcgaat tctgcagtcg acggtaccgc gggcccggga tccaccggtc
       61 gccaccatgg tgagcaaggg cgaggaggat aacatggcca tcatcaagga gttcatgcgc
      121 ttcaaggtgc acatggaggg ctccgtgaac ggccacgagt tcgagatcga gggcgagggc
      181 gagggccgcc cctacgaggg cacccagacc gccaagctga aggtgaccaa gggtggcccc
      241 ctgcccttcg cctgggacat cctgtcccct cagttcatgt acggctccaa ggcctacgtg
      301 aagcaccccg ccgacatccc cgactacttg aagctgtcct tccccgaggg cttcaagtgg
      361 gagcgcgtga tgaacttcga ggacggcggc gtggtgaccg tgacccagga ctcctccctg
      421 caggacggcg agttcatcta caaggtgaag ctgcgcggca ccaacttccc ctccgacggc
      481 cccgtaatgc agaagaagac catgggctgg gaggcctcct ccgagcggat gtaccccgag
      541 gacggcgccc tgaagggcga gatcaagcag aggctgaagc tgaaggacgg cggccactac
      601 gacgctgagg tcaagaccac ctacaaggcc aagaagcccg tgcagctgcc cggcgcctac
      661 aacgtcaaca tcaagttgga catcacctcc cacaacgagg actacaccat cgtggaacag
      721 tacgaacgcg ccgagggccg ccactccacc ggcggcatgg acgagctgta caagtag
//
