LOCUS       sequence-411092-        4782 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Lentiviral expression vector encoding FIP200 mutant-mCherry.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 4782)
  AUTHORS   Chen M, Ren X, Nguyen TN, Khuu G, Cook ASI, Lazarou M, Hurley JH
  TITLE     Structure and activation of the human autophagy-initiating 
            ULK1C:PI3KC3-C1 supercomplex
  JOURNAL   bioRxiv 2023.06.01.543278
REFERENCE   2  (bases 1 to 4782)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "bioRxiv 
            2023.06.01.543278"
COMMENT     Sequence Label: 
            pCDH1-CMV-FIP200-V44D/V314D/L324D/R543D/R548D-mCherry-Hygro
FEATURES             Location/Qualifiers
     source          1..4782
                     /mol_type="genomic DNA"
                     /organism="unspecified"
ORIGIN
        1 atgaagctct atgtgtttct cgtgaatacc ggcactaccc tgacctttga taccgaactg
       61 accgtccaga ccgtggctga cctgaagcat gctatccagt ctaaatacaa gatcgcaatt
      121 cagcaccagg acctggtggt caacggcgga gagtgcatgg ccgctgatcg cagggtctgt
      181 acctattcag ccggcaccga cactaaccca atcttcctct ttaataagga gatgattctg
      241 tgcgatagac cccctgctat ccccaagacc acttttagca ctgagaacga catggaaatc
      301 aaagtggagg aatccctgat gatgccagcc gtgttccaca ccgtcgcttc tcgcactcag
      361 ctggcactcg agatgtacga agtggccaag aaactgtgct ctttctgtga gggtctcgtc
      421 cacgatgaac atctgcagca ccagggctgg gcagccatta tggctaacct ggaggactgt
      481 tcaaatagtt atcagaaact gctcttcaag tttgagtcta tctactcaaa ctatctgcag
      541 agcatcgaag acattaaact gaagctcaca cacctgggaa ccgcagtgag cgtcatggcc
      601 aagatccctc tgctcgagtg cctgactagg cattcctaca gagaatgtct gggtaggctc
      661 gatagtctgc cagaacacga ggacagcgag aaagccgaaa tgaagcgctc tacagagctg
      721 gtgctctcac ctgacatgcc aaggacaacc aacgaaagtc tgctcaccag cttcccaaag
      781 tccgtggagc atgtctcccc cgacactgcc gatgctgaat ctggaaagga gattagagaa
      841 agttgccaga gcaccgtgca ccagcaggac gagactacaa ttgacactaa ggacggcgat
      901 ctgcccttct ttaacgtcag tctgctcgat tggatcaacg accaggacag acctaatgat
      961 gtggagtccg acgtccggaa gtgctttgat agcatgtccc gtctggaccc acgtatcatt
     1021 cgtcctttca tcgcagagtg tcgtcagacc attgccaagc tggacaacca gaatatgaaa
     1081 gctattaagg gtctggaaga tcggctctac gccctggacc agatgatcgc tagctgtgga
     1141 cgcctggtga acgagcagaa ggaactcgct cagggtttcc tggcaaacca gaaaagagcc
     1201 gagaatctca aggatgcatc agtgctgcct gacctgtgcc tcagtcatgc caatcagctg
     1261 atgattatgc tccagaacca ccgcaagctg ctcgacatca aacagaagtg taccactgct
     1321 aaacaggagc tggcaaacaa tctccatgtg aggctgaagt ggtgctgttt cgtcatgctg
     1381 cacgcagacc aggatggcga gaagctccag gccctgctca ggctggtgat tgagctgctc
     1441 gaaagagtga agatcgtcga ggctctgtcc accgtcccac agatgtactg cctggcagtg
     1501 gtcgaggtgg tcagacgtaa aatgtttatc aagcactaca gagaatgggc aggtgccctc
     1561 gtgaaagatg gcaagcgtct gtatgaagcc gagaaatcta agcgtgagtc attcggcaaa
     1621 ctgtttgaca agagcttcct cgataaccgg ctgttccgcg gactcgacag ttggccaccc
     1681 agcttttgca cccagaaacc taggaagttc gactgtgagc tgccagatat ctccctcaag
     1741 gacctgcagt ttctccagtc cttctgccca tctgaggtgc agccctttct gagggtccct
     1801 ctgctctgtg acttcgaacc tctgcaccag catgtgctgg ccctccacaa cctcgtcaag
     1861 gctgcacaga gtctggatga gatgagccag acaatcaccg acctgctcag cgaacagaag
     1921 gcttctgtgt cacagacatc cccacagtct gctagctccc cccgtatgga gagcaccgca
     1981 ggcatcacaa ccactacatc cccacgtaca cctccaccac tcaccgtgca ggatcccctg
     2041 tgccctgctg tctgtcctct ggaggaactc tctccagaca gtattgatgc acacactttc
     2101 gactttgaga caatccctca tccaaacatc gaacagacca ttcaccaggt gtccctggac
     2161 ctcgattctc tggccgagag ccccgaatcc gattttatgt ccgctgtgaa tgagttcgtc
     2221 attgaggaaa acctgtcttc acccaatcct atctcagacc cacagagtcc cgagatgatg
     2281 gtggaatccc tgtacagtag cgtcatcaac gccattgatt ctcggcgcat gcaggacaca
     2341 aatgtgtgcg gcaaggagga ctttggagat catacctctc tgaacgtcca gctcgaacgc
     2401 tgtagggtgg tcgctcagga ttctcatttc tcaatccaga ctattaagga ggacctgtgc
     2461 cacttccgca catttgtgca gaaggaacag tgcgacttca gtaacagcct gaaatgtaca
     2521 gccgtggaga tcaggaacat catcgaaaag gtcaagtgtt ctctggagat caccctcaaa
     2581 gaaaagcacc agaaagagct gctctcactg aagaacgaat acgagggaaa actggacggt
     2641 ctcattaagg aaaccgagga aaacgagaat aaaatcaaga aactgaaggg agagctggtg
     2701 tgcctcgagg aagtcctgca gaacaaggat aatgaattcg ccctggtgaa acatgaaaag
     2761 gaggctgtca tctgcctgca gaatgagaaa gaccagaagc tgctcgaaat ggagaacatt
     2821 atgcactcac agaattgtga gatcaaagaa ctgaagcaga gtagagagat cgtgctggaa
     2881 gatctcaaga aactgcatgt cgagaatgac gaaaagctgc agctgctccg tgccgaactc
     2941 cagagcctgg agcagtccca cctcaaggaa ctggaggata ctctgcaggt gcgccatatc
     3001 caggaattcg agaaggtgat gacagaccac agagtcagcc tcgaggaact gaagaaagag
     3061 aaccagcaga tcattaatca gatccaggag tcccacgctg aaatcattca ggaaaaagag
     3121 aagcagctgc aggagctgaa actcaaggtg tctgacctca gcgatacccg ctgcaaactg
     3181 gaagtcgagc tggccctcaa ggaagctgag actgacgaga tcaaaattct gctcgaggaa
     3241 tctcgggccc agcagaaaga aaccctgaag tcactgctcg aacaggagac cgaaaatctc
     3301 cgtactgaga tcagcaaact gaaccagaag attcaggata acaatgagaa ttaccaagtg
     3361 ggactcgctg aactgagaac tctcatgaca atcgagaagg accagtgtat ctctgaactg
     3421 atttcacgtc atgaggaaga gtcaaacatt ctgaaagcag agctcaataa agtgactagt
     3481 ctgcacaacc aggccttcga gatcgagaag aacctgaagg agcagatcat tgaactgcag
     3541 tccaagctcg attccgaact gtctgccctc gagaggcaga aagacgaaaa gatcacacag
     3601 caggaagaga agtatgaggc tatcattcag aacctcgaaa aagataggca gaagctggtg
     3661 tcctctcagg agcaggacag agaacagctg attcagaaac tcaattgcga gaaggacgaa
     3721 gcaatccaga cagccctcaa agagttcaag ctggagagag aagtggtcga gaaagaactg
     3781 ctcgagaaag tgaagcacct ggaaaaccag atcgctaagt cacccgcaat tgatagtacc
     3841 cgtggtgact caagtagcct ggtggccgag ctccaggaaa agctgcagga agagaaggct
     3901 aagtttctgg agcagctcga agagcaggaa aaaagaaaga acgaagagat gcagaatgtg
     3961 cgtacaagcc tgatcgccga gcagcagacc aacttcaata ccgtcctgac tcgtgagaaa
     4021 atgcggaagg aaaacatcat taatgacctc agcgataaac tgaagtccac catgcagcag
     4081 caggagcgcg acaaggatct catcgagtcc ctgtctgaag acagagctcg tctgctcgaa
     4141 gagaagaaaa agctggaaga ggaagtcagt aagctgaggt cctcttcatt cgtgccaagc
     4201 ccctacgtcg ctactgcacc cgagctgtat ggagcctgcg ctcctgagct gcctggtgaa
     4261 tcagaccgga gtgccgtgga gactgctgat gaaggccgcg tcgactctgc catggagaca
     4321 agcatgatgt ccgtgcagga aaacattcat atgctgtcag aggaaaagca gagaatcatg
     4381 ctgctcgagc gtaccctgca gctcaaagag gaagagaaca agcgcctcaa tcagaggctg
     4441 atgtcacaga gtatgagtag cgtgtcctct cgccactccg agaagatcgc cattagggat
     4501 ttccaagtgg gagacctggt cctcatcatt ctggacgaac gccatgataa ctacgtgctg
     4561 tttactgtct ctcctacact gtatttcctc cacagcgagt ccctgcccgc tctggacctc
     4621 aagcctggtg aaggcgcatc tggcgcctca aggagaccct gggtgctggg aaaagtcatg
     4681 gagaaggaat actgccaggc taaaaaggca cagaaccgtt ttaaggtgcc tctgggcacc
     4741 aaattctatc gggtgaaggc cgtctcctgg aataaaaagg tc
//
