LOCUS       sequence-411099-        9262 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses E-cadherin with a LOV2 insert after T133 for blue light 
            inhibition in mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9262)
  AUTHORS   Nzigou Mombo B, Bijonowski BM, Raab CA, Niland S, Brockhaus K, 
            Muller M, Eble JA, Wegner SV
  TITLE     Reversible photoregulation of cell-cell adhesions with 
            opto-E-cadherin.
  JOURNAL   Nat Commun. 2023 Oct 9;14(1):6292. doi: 10.1038/s41467-023-41932-0.
  PUBMED    37813868
REFERENCE   2  (bases 1 to 9262)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2023-10-9"; volume: "14(1):6292. doi"; pages: " 
            10.1038/s41467-023-41932-0"
COMMENT     Sequence Label: opto-E-cad GFP
FEATURES             Location/Qualifiers
     source          1..9262
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(44..63)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        235..614
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        615..818
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     769..789
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     863..882
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        863..881
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             4086..4598
                     /codon_start=1
                     /product="N-terminal fragment of mVenus for use in 
                     bimolecular fluorescence complementation (BiFC) (Kodama and
                     Hu, 2010)"
                     /label=VN173
                     /translation="SKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLKF
                     ICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDDGN
                     YKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKVN
                     FKIRHNIE"
     primer_bind     complement(4125..4146)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(4386..4405)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     4733..4754
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     promoter        complement(4815..4833)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(4816..4833)
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     complement(4853..4870)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    4859..5083
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      5129..5557
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(5216..5235)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     5426..5447
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(5566..5586)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        5571..5900
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      5751..5886
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     5813..5832
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     CDS             5967..6761
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(6021..6040)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     6631..6650
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     polyA_signal    6935..7056
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(6972..6991)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     7026..7045
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(7105..7121)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(7105..7121)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(7118..7140)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    7129..7145
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(7153..7159,7160..7177,7178..7183))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    7198..7219
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(7336..7353)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(7507..8095)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(7587..7606)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(8266..9057,9058..9126))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     8889..8908
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(9127..9231)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 gacggatcgg gagatctccc gatcccctat ggtgcactct cagtacaatc tgctctgatg
       61 ccgcatagtt aagccagtat ctgctccctg cttgtgtgtt ggaggtcgct gagtagtgcg
      121 cgagcaaaat ttaagctaca acaaggcaag gcttgaccga caattgcatg aagaatctgc
      181 ttagggttag gcgttttgcg ctgcttcgcg atgtacgggc cagatatacg cgttgacatt
      241 gattattgac tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata
      301 tggagttccg cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc
      361 cccgcccatt gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc
      421 attgacgtca atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt
      481 atcatatgcc aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt
      541 atgcccagta catgacctta tgggactttc ctacttggca gtacatctac gtattagtca
      601 tcgctattac catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg
      661 actcacgggg atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc
      721 aaaatcaacg ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg
      781 gtaggcgtgt acggtgggag gtctatataa gcagagctct ctggctaact agagaaccca
      841 ctgcttactg gcttatcgaa attaatacga ctcactatag ggagacccaa gcttgatatc
      901 gaattccgga aagcacctgt gagcttgcgg aagtcagttc agactccagc ccgctccagc
      961 ccggcccgac ccgaccgcac ccggcgcctg ccctcgctcg gcgtccccgg ccagccatgg
     1021 gcccttggag ccgcagcctc tcggcgctgc tgctgctgct gcaggtctcc tcttggctct
     1081 gccaggagcc ggagccctgc caccctggct ttgacgccga gagctacacg ttcacggtgc
     1141 cccggcgcca cctggagaga ggccgcgtcc tgggcagagt gaattttgaa gattgcaccg
     1201 gtcgacaaag gacagcctat ttttccctcg acacccgatt caaagtgggc acagatggtg
     1261 tgattacagt caaaaggcct ctacggtttc ataacccaca gatccatttc ttggtctacg
     1321 cctgggactc cacctacaga aagttttcca ccaaagtcac gctgaataca gtggggcacc
     1381 accaccgccc cccgccccat caggcctccg tttctggaat ccaagcagaa ttgctcacat
     1441 ttcccaactc ctctcctggc ctcagaagac agaagagaga ctgggttatt cctcccatca
     1501 gctgcccaga aaatgaaaaa ggcccatttc ctaaaaacct ggttcagatc aaatccaaca
     1561 aagacaaaga aggcaaggtt ttctacagca tcactggcca aggagctgac acaccccctg
     1621 ttggtgtctt tattattgaa agagaaacag gatggctgaa ggtgacagag cctctggata
     1681 gagaacgcat tgccacatac actctcttct ctcacgctgt gtcatccaac gggaatgcag
     1741 ttgaggatcc aatggagatt ttgatcacgg taaccgatca gaatgacaac aagcccgaat
     1801 tcacccagga ggtctttaag gggtctgtca tggaaggtgc tcttccagga acctctgtga
     1861 tggaggtcac agccacactg gcgaccaccc tggaacgtat tgaaaagaac tttgtgatta
     1921 ccgatccgcg tctgccggat aacccgatta tttttgcgag cgatagcttt ctgcagctga
     1981 ccgaatatag ccgtgaagaa attctgggcc gtaactgccg ttttctgcag ggcccggaaa
     2041 ccgatcgtgc gaccgtgcgt aaaattcgtg atgcgattga taaccagacc gaagtgaccg
     2101 tgcagctgat taactatacc aaaagcggca aaaaattttg gaacctgttt catctgcagc
     2161 cgatgcgtga tcagaaaggc gatgtgcagt attttattgg cgtgcagctg gatggtaccg
     2221 aacatgtgcg tgatgcggcg gaacgtgaag gcgtgatgct gatcaagaaa accgcggaaa
     2281 acattgatga agcggacgcg gacgatgatg tgaacaccta caatgccgcc atcgcttaca
     2341 ccatcctcag ccaagatcct gagctccctg acaaaaatat gttcaccatt aacaggaaca
     2401 caggagtcat cagtgtggtc accactgggc tggaccgaga gagtttccct acgtataccc
     2461 tggtggttca agctgctgac cttcaaggtg aggggttaag cacaacagca acagctgtga
     2521 tcacagtcac tgacaccaac gataatcctc cgatcttcaa tcccaccacg tacaagggtc
     2581 aggtgcctga gaacgaggct aacgtcgtaa tcaccacact gaaagtgact gatgctgatg
     2641 cccccaatac cccagcgtgg gaggctgtat acaccatatt gaatgatgat ggtggacaat
     2701 ttgtcgtcac cacaaatcca gtgaacaacg atggcatttt gaaaacagca aagggcttgg
     2761 attttgaggc caagcagcag tacattctac acgtagcagt gacgaatgtg gtaccttttg
     2821 aggtctctct caccacctcc acagccaccg tcaccgtgga tgtgctggat gtgaatgaag
     2881 cccccatctt tgtgcctcct gaaaagagag tggaagtgtc cgaggacttt ggcgtgggcc
     2941 aggaaatcac atcctacact gcccaggagc cagacacatt tatggaacag aaaataacat
     3001 atcggatttg gagagacact gccaactggc tggagattaa tccggacact ggtgccattt
     3061 ccactcgggc tgagctggac agggaggatt ttgagcacgt gaagaacagc acgtacacag
     3121 ccctaatcat agctacagac aatggttctc cagttgctac tggaacaggg acacttctgc
     3181 tgatcctgtc tgatgtgaat gacaacgccc ccataccaga acctcgaact atattcttct
     3241 gtgagaggaa tccaaagcct caggtcataa acatcattga tgcagacctt cctcccaata
     3301 catctccctt cacagcagaa ctaacacacg gggcgagtgc caactggacc attcagtaca
     3361 acgacccaac ccaagaatct atcattttga agccaaagat ggccttagag gtgggtgact
     3421 acaaaatcaa tctcaagctc atggataacc agaataaaga ccaagtgacc accttagagg
     3481 tcagcgtgtg tgactgtgaa ggggccgccg gcgtctgtag gaaggcacag cctgtcgaag
     3541 caggattgca aattcctgcc attctgggga ttcttggagg aattcttgct ttgctaattc
     3601 tgattctgct gctcttgctg tttcttcgga ggagagcggt ggtcaaagag cccttactgc
     3661 ccccagagga tgacacccgg gacaacgttt attactatga tgaagaagga ggcggagaag
     3721 aggaccagga ctttgacttg agccagctgc acaggggcct ggacgctcgg cctgaagtga
     3781 ctcgtaacga cgttgcacca accctcatga gtgtcccccg gtatcttccc cgccctgcca
     3841 atcccgatga aattggaaat tttattgatg aaaatctgaa agcggctgat actgacccca
     3901 cagccccgcc ttatgattct ctgctcgtgt ttgactatga aggaagcggt tccgaagctg
     3961 ctagtctgag ctccctgaac tcctcagagt cagacaaaga ccaggactat gactacttga
     4021 acgaatgggg caatcgcttc aagaagctgg ctgacatgta cggaggcggc gaggacgacc
     4081 cgcggagcaa gggggaggag ctgttcaccg gggtggtgcc catcctggtc gagctggacg
     4141 gcgacgtaaa cggccacaag ttcagcgtgt ccggcgaggg cgagggcgat gccacctacg
     4201 gcaagctgac cctgaagttc atctgcacca ccggcaagct gcccgtgccc tggcccaccc
     4261 tcgtgaccac cctgacctac ggcgtgcagt gcttcagccg ctaccccgac cacatgaagc
     4321 agcacgactt cttcaagtcc gccatgcccg aaggctacgt ccaggagcgc accatcttct
     4381 tcaaggacga cggcaactac aagacccgcg ccgaggtgaa gttcgagggc gacaccctgg
     4441 tgaaccgcat cgagctgaag ggcatcgact tcaaggagga cggcaacatc ctggggcaca
     4501 agctggagta caactacaac agccacaacg tctatatcat ggccgacaag cagaagaacg
     4561 gcatcaaggt gaacttcaag atccgccaca acatcgagga cggcagcgtg cagctcgccg
     4621 accactacca gcagaacacc cccatcggcg acggccccgt gctgctgccc gacaaccact
     4681 acctgagcac ccagtccgcc ctgagcaaag accccaacga gaagcgcgat cacatggtcc
     4741 tgctggagtt cgtgaccgcc gccgggatca ctctcggcat ggacgagctg atcaagtaat
     4801 ctagagggcc ctattctata gtgtcaccta aatgctagag ctcgctgatc agcctcgact
     4861 gtgccttcta gttgccagcc atctgttgtt tgcccctccc ccgtgccttc cttgaccctg
     4921 gaaggtgcca ctcccactgt cctttcctaa taaaatgagg aaattgcatc gcattgtctg
     4981 agtaggtgtc attctattct ggggggtggg gtggggcagg acagcaaggg ggaggattgg
     5041 gaagacaata gcaggcatgc tggggatgcg gtgggctcta tggcttctga ggcggaaaga
     5101 accagctggg gctctagggg gtatccccac gcgccctgta gcggcgcatt aagcgcggcg
     5161 ggtgtggtgg ttacgcgcag cgtgaccgct acacttgcca gcgccctagc gcccgctcct
     5221 ttcgctttct tcccttcctt tctcgccacg ttcgccggct ttccccgtca agctctaaat
     5281 cgggggctcc ctttagggtt ccgatttagt gctttacggc acctcgaccc caaaaaactt
     5341 gattagggtg atggttcacg tagtgggcca tcgccctgat agacggtttt tcgccctttg
     5401 acgttggagt ccacgttctt taatagtgga ctcttgttcc aaactggaac aacactcaac
     5461 cctatctcgg tctattcttt tgatttataa gggattttgc cgatttcggc ctattggtta
     5521 aaaaatgagc tgatttaaca aaaatttaac gcgaattaat tctgtggaat gtgtgtcagt
     5581 tagggtgtgg aaagtcccca ggctccccag caggcagaag tatgcaaagc atgcatctca
     5641 attagtcagc aaccaggtgt ggaaagtccc caggctcccc agcaggcaga agtatgcaaa
     5701 gcatgcatct caattagtca gcaaccatag tcccgcccct aactccgccc atcccgcccc
     5761 taactccgcc cagttccgcc cattctccgc cccatggctg actaattttt tttatttatg
     5821 cagaggccga ggccgcctct gcctctgagc tattccagaa gtagtgagga ggcttttttg
     5881 gaggcctagg cttttgcaaa aagctcccgg gagcttgtat atccattttc ggatctgatc
     5941 aagagacagg atgaggatcg tttcgcatga ttgaacaaga tggattgcac gcaggttctc
     6001 cggccgcttg ggtggagagg ctattcggct atgactgggc acaacagaca atcggctgct
     6061 ctgatgccgc cgtgttccgg ctgtcagcgc aggggcgccc ggttcttttt gtcaagaccg
     6121 acctgtccgg tgccctgaat gaactgcagg acgaggcagc gcggctatcg tggctggcca
     6181 cgacgggcgt tccttgcgca gctgtgctcg acgttgtcac tgaagcggga agggactggc
     6241 tgctattggg cgaagtgccg gggcaggatc tcctgtcatc tcaccttgct cctgccgaga
     6301 aagtatccat catggctgat gcaatgcggc ggctgcatac gcttgatccg gctacctgcc
     6361 cattcgacca ccaagcgaaa catcgcatcg agcgagcacg tactcggatg gaagccggtc
     6421 ttgtcgatca ggatgatctg gacgaagagc atcaggggct cgcgccagcc gaactgttcg
     6481 ccaggctcaa ggcgcgcatg cccgacggcg aggatctcgt cgtgacccat ggcgatgcct
     6541 gcttgccgaa tatcatggtg gaaaatggcc gcttttctgg attcatcgac tgtggccggc
     6601 tgggtgtggc ggaccgctat caggacatag cgttggctac ccgtgatatt gctgaagagc
     6661 ttggcggcga atgggctgac cgcttcctcg tgctttacgg tatcgccgct cccgattcgc
     6721 agcgcatcgc cttctatcgc cttcttgacg agttcttctg agcgggactc tggggttcga
     6781 aatgaccgac caagcgacgc ccaacctgcc atcacgagat ttcgattcca ccgccgcctt
     6841 ctatgaaagg ttgggcttcg gaatcgtttt ccgggacgcc ggctggatga tcctccagcg
     6901 cggggatctc atgctggagt tcttcgccca ccccaacttg tttattgcag cttataatgg
     6961 ttacaaataa agcaatagca tcacaaattt cacaaataaa gcattttttt cactgcattc
     7021 tagttgtggt ttgtccaaac tcatcaatgt atcttatcat gtctgtatac cgtcgacctc
     7081 tagctagagc ttggcgtaat catggtcata gctgtttcct gtgtgaaatt gttatccgct
     7141 cacaattcca cacaacatac gagccggaag cataaagtgt aaagcctggg gtgcctaatg
     7201 agtgagctaa ctcacattaa ttgcgttgcg ctcactgccc gctttccagt cgggaaacct
     7261 gtcgtgccag ctgcattaat gaatcggcca acgcgcgggg agaggcggtt tgcgtattgg
     7321 gcgctcttcc gcttcctcgc tcactgactc gctgcgctcg gtcgttcggc tgcggcgagc
     7381 ggtatcagct cactcaaagg cggtaatacg gttatccaca gaatcagggg ataacgcagg
     7441 aaagaacatg tgagcaaaag gccagcaaaa ggccaggaac cgtaaaaagg ccgcgttgct
     7501 ggcgtttttc cataggctcc gcccccctga cgagcatcac aaaaatcgac gctcaagtca
     7561 gaggtggcga aacccgacag gactataaag ataccaggcg tttccccctg gaagctccct
     7621 cgtgcgctct cctgttccga ccctgccgct taccggatac ctgtccgcct ttctcccttc
     7681 gggaagcgtg gcgctttctc atagctcacg ctgtaggtat ctcagttcgg tgtaggtcgt
     7741 tcgctccaag ctgggctgtg tgcacgaacc ccccgttcag cccgaccgct gcgccttatc
     7801 cggtaactat cgtcttgagt ccaacccggt aagacacgac ttatcgccac tggcagcagc
     7861 cactggtaac aggattagca gagcgaggta tgtaggcggt gctacagagt tcttgaagtg
     7921 gtggcctaac tacggctaca ctagaagaac agtatttggt atctgcgctc tgctgaagcc
     7981 agttaccttc ggaaaaagag ttggtagctc ttgatccggc aaacaaacca ccgctggtag
     8041 cggtggtttt tttgtttgca agcagcagat tacgcgcaga aaaaaaggat ctcaagaaga
     8101 tcctttgatc ttttctacgg ggtctgacgc tcagtggaac gaaaactcac gttaagggat
     8161 tttggtcatg agattatcaa aaaggatctt cacctagatc cttttaaatt aaaaatgaag
     8221 ttttaaatca atctaaagta tatatgagta aacttggtct gacagttacc aatgcttaat
     8281 cagtgaggca cctatctcag cgatctgtct atttcgttca tccatagttg cctgactccc
     8341 cgtcgtgtag ataactacga tacgggaggg cttaccatct ggccccagtg ctgcaatgat
     8401 accgcgagac ccacgctcac cggctccaga tttatcagca ataaaccagc cagccggaag
     8461 ggccgagcgc agaagtggtc ctgcaacttt atccgcctcc atccagtcta ttaattgttg
     8521 ccgggaagct agagtaagta gttcgccagt taatagtttg cgcaacgttg ttgccattgc
     8581 tacaggcatc gtggtgtcac gctcgtcgtt tggtatggct tcattcagct ccggttccca
     8641 acgatcaagg cgagttacat gatcccccat gttgtgcaaa aaagcggtta gctccttcgg
     8701 tcctccgatc gttgtcagaa gtaagttggc cgcagtgtta tcactcatgg ttatggcagc
     8761 actgcataat tctcttactg tcatgccatc cgtaagatgc ttttctgtga ctggtgagta
     8821 ctcaaccaag tcattctgag aatagtgtat gcggcgaccg agttgctctt gcccggcgtc
     8881 aatacgggat aataccgcgc cacatagcag aactttaaaa gtgctcatca ttggaaaacg
     8941 ttcttcgggg cgaaaactct caaggatctt accgctgttg agatccagtt cgatgtaacc
     9001 cactcgtgca cccaactgat cttcagcatc ttttactttc accagcgttt ctgggtgagc
     9061 aaaaacagga aggcaaaatg ccgcaaaaaa gggaataagg gcgacacgga aatgttgaat
     9121 actcatactc ttcctttttc aatattattg aagcatttat cagggttatt gtctcatgag
     9181 cggatacata tttgaatgta tttagaaaaa taaacaaata ggggttccgc gcacatttcc
     9241 ccgaaaagtg ccacctgacg tc
//
