LOCUS       sequence-411119-       15373 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses the genetically-encoded fluorescent norepinephrine (NE) 
            sensor GRAB_NE2h pan-neuronally in zebrafish.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 15373)
  AUTHORS   Feng J, Dong H, Lischinsky JE, Zhou J, Deng F, Zhuang C, Miao X, 
            Wang H, Li G, Cai R, Xie H, Cui G, Lin D, Li Y
  TITLE     Monitoring norepinephrine release in vivo using next-generation 
            GRAB(NE) sensors.
  JOURNAL   Neuron. 2024 Jun 19;112(12):1930-1942.e6. doi: 
            10.1016/j.neuron.2024.03.001. Epub 2024 Mar 27.
  PUBMED    38547869
REFERENCE   2  (bases 1 to 15373)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.neuron.2024.03.001"; journalName: "Neuron"; date: 
            "2024-06-19- 19"; volume: "112"; issue: "12"; pages: "1930-1942.e6"
COMMENT     Sequence Label: pminiTol2-HuC-NE2h
FEATURES             Location/Qualifiers
     source          1..15373
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(24..41)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(24..40)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(33..55)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      181..636
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     promoter        714..818
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(819..887,888..1679)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(1037..1056)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      1850..2438
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     2339..2358
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     2592..2609
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    2726..2747
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(2762..2767,2768..2785,2786..2792)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    2800..2816
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     2805..2827
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     2824..2840
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     2824..2840
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     promoter        2858..2876
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     2858..2875
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     misc_feature    join(2927..2938,2939..3101)
                     /label=Tol2 right end
                     /note="right end of the Tol2 transposon from the medaka 
                     fish Oryzias latipes (Kawakami, 2007)"
     sig_peptide     11928..11987
                     /codon_start=1
                     /product="leader sequence from mouse immunoglobulin kappa 
                     light chain"
                     /label=Ig-kappa leader
                     /translation="METDTLLLWVLLLWVPGSTG"
     protein_bind    11991..12015
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     primer_bind     13112..13133
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     primer_bind     complement(13239..13260)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(13500..13519)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     polyA_signal    14041..14162
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(14078..14097)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     14132..14151
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      complement(14169..14624)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     promoter        14651..14755
                     /gene="bla"
                     /label=AmpR promoter
     enhancer        14757..14948
                     /label=SV40 enhancer
                     /note="enhancer for the SV40 early promoter (Herr, 1993)"
     primer_bind     complement(14780..14800)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        14798..15095
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     misc_feature    join(15160..15347,15348..15359)
                     /label=Tol2 left end
                     /note="left end of the Tol2 transposon from the medaka fish
                     Oryzias latipes (Kawakami, 2007)"
     primer_bind     complement(join(15371..15373,1..17))
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(join(15372..15373,1..17))
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
ORIGIN
        1 tatagtgagt cgtattacaa ttcactggcc gtcgttttac aacgtcgtga ctgggaaaac
       61 cctggcgtta cccaacttaa tcgccttgca gcacatcccc ctttcgccag ctggcgtaat
      121 agcgaagagg cccgcaccga tcgcccttcc caacagttgc gcagcctgaa tggcgaatgg
      181 acgcgccctg tagcggcgca ttaagcgcgg cgggtgtggt ggttacgcgc agcgtgaccg
      241 ctacacttgc cagcgcccta gcgcccgctc ctttcgcttt cttcccttcc tttctcgcca
      301 cgttcgccgg ctttccccgt caagctctaa atcgggggct ccctttaggg ttccgattta
      361 gtgctttacg gcacctcgac cccaaaaaac ttgattaggg tgatggttca cgtagtgggc
      421 catcgccctg atagacggtt tttcgccctt tgacgttgga gtccacgttc tttaatagtg
      481 gactcttgtt ccaaactgga acaacactca accctatctc ggtctattct tttgatttat
      541 aagggatttt gccgatttcg gcctattggt taaaaaatga gctgatttaa caaaaattta
      601 acgcgaattt taacaaaata ttaacgctta caatttcctg atgcggtatt ttctccttac
      661 gcatctgtgc ggtatttcac accgcatcag gtggcacttt tcggggaaat gtgcgcggaa
      721 cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac
      781 cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg
      841 tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc
      901 tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg
      961 atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga
     1021 gcacttttaa agttctgcta tgtggcgcgg tattatcccg tattgacgcc gggcaagagc
     1081 aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag
     1141 aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga
     1201 gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg
     1261 cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga
     1321 atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgtagcaatg gcaacaacgt
     1381 tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact
     1441 ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt
     1501 ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg
     1561 ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta
     1621 tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac
     1681 tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta
     1741 aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt
     1801 tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt
     1861 tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt
     1921 gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc
     1981 agataccaaa tactgttctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg
     2041 tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg
     2101 ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt
     2161 cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac
     2221 tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg
     2281 acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg
     2341 gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat
     2401 ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt
     2461 tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg
     2521 attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa
     2581 cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcccaata cgcaaaccgc
     2641 ctctccccgc gcgttggccg attcattaat gcagctggca cgacaggttt cccgactgga
     2701 aagcgggcag tgagcgcaac gcaattaatg tgagttagct cactcattag gcaccccagg
     2761 ctttacactt tatgcttccg gctcgtatgt tgtgtggaat tgtgagcgga taacaatttc
     2821 acacaggaaa cagctatgac catgattacg ccaagctatt taggtgacac tatagaatac
     2881 tcaagctatg catccaacgc gttgggagct ctcccatatg gtcgagcaga ggtgtaaaaa
     2941 gtactcaaaa attttactca agtgaaagta caagtactta gggaaaattt tactcaatta
     3001 aaagtaaaag tatctggcta gaatcttact tgagtaaaag taaaaaagta ctccattaaa
     3061 attgtacttg agtattaagg aagtaaaagt aaaagcaaga aagaaaacta gagattcttg
     3121 tttaagcttg gatccgtacc gggccccccc tcgaaattca ctaatttgaa tttaaatgca
     3181 ttatctttct attcctaaag accttgggtg accaaaatct tattttaata aataaaactg
     3241 tttattaaaa cttttttgtt tcaaagaacc atatgtatag tgaaatttat aaaaatatca
     3301 atttttaaaa agctggtgta ctcatttatg ttatgaactc taaaaccata tactgactgc
     3361 aagtgatgat gtatagagtg atgtttacga gtaaacatat ttagttgtat acatcctact
     3421 gagcacattt tgatgtatga aataacatta caagctttat ccaaattaag ccattttaaa
     3481 acactgccaa ttgaaaatac aaatcctgga aaaaatcgtc tttagcgcag tcatttgagc
     3541 catcctaatc cgttacctca gaccataata agaagggata acactagctg tagcaatgga
     3601 acacatctgt ttcacacaat catatctcct gcgccggtgc taagcagatt cagcgtgatc
     3661 ataacatgct ttccactcat aaatgtaaat ttacaatttg cacatgtaaa acagacactt
     3721 ttgagatatt ggataaaaaa acaagagtat attgcttagt ttcatccacc agtcatcccc
     3781 acagcgtttg gaaggccata aaaagtgtct aaaatcaatg atcattgaaa gagcacaaga
     3841 gagactctta cgctgtaatg ccactgggga caaaagtgac agtctcttaa tgggctcttc
     3901 tggaggggct cctgaacatt aaaaattatc agcgaaatta ccgaaagagc ttcaagcaac
     3961 tggcatgctt gatcctctgc gtcggggcgg tgaataggtg cttcagatgc cctcttaccc
     4021 acgggctgga ttcagctgcc ccgctaccag cggagacccc ctaatgagcc tctgcaatta
     4081 agtttattca tgttaagtgt gaacggggtg cgtgcggaac tgtgggcagc taacagacct
     4141 gggttctttg tgccacaagt gctgccttta ttcggctcac aaagcagaaa acaacacccg
     4201 cacctataat ggcgccctcg gctgggtcta agaaacgtgg cgagttgaca gagcagagtg
     4261 ggcggggtta agacagactg acagcgggac ccatctccat cctcttatta acgcttaacg
     4321 agtgccttcc tcatgcaata ttcatcgcca ctaatatcat ccaagctctg agctgagctg
     4381 gccacttatg taaggcaatt atgtaaaata tcagacaggg cccacactca gaatctgact
     4441 ggggtagaga cgcgggacga gaaccgagag caagaactga aagtgaaagt gaccactaaa
     4501 gggaggagag gacagagggg caggatgtgt caagattacc agagaacact tggccagaaa
     4561 tgcgcaacca ttggagctct ccggattacc caaaggttaa cgagtttgaa cgcctctgcc
     4621 cactcgccca tctctgatgg tttcccaaga actcctcaag caaaatatat ataattgtgt
     4681 gtattatgca cagacacgag aaaatgctgt ttttctgatc tgcattacag cacatttgcc
     4741 cgccaacgac aataccaccc actcggtacc tcgctgactc ctgatgcctg atacctgcgc
     4801 ggtgactgtc tacaatctgc ataatcaaga gaagttgtgt tgaagacgag cgccacacaa
     4861 ccgtttccac aaggtcaccc aaggccggtg cagatgtagg tgaggtctcc ataaacagac
     4921 tgaaataaac acatcctccg ctgggaacaa caaccccctc acgcctcatg catttccata
     4981 agcctacatg catctcttcc aacttatgga gactcgcacc taccaacatc cgcacaacaa
     5041 agatatacag agcgcgctcc ctcaggtcaa ggccctgtgg gggtctgtgc agaaataggt
     5101 catttgtcac acatcaagtc ctggggcagg agatgcatta tagatgagac caaacagcct
     5161 gtctcggtga gctctaccca ctccctgaga ctagaaatgg gggaagggag cttgagataa
     5221 caaccgctgc aatcactgtg tcgatgttta atatcagcac caaccgggaa caataaggag
     5281 atgggtgcat tcatgttcac atcttaccag tcaagtatca tcgaaccggc ttgataacca
     5341 cacctcgtgt aatagctgag cagatagttg tcattttaaa gcgttggcct ttgtcgatta
     5401 tgtaatgcgc acattcaaca catggtaata tagaaacggt tatgtcgagg ttgttttgtc
     5461 cagagatgac cttcacacag ttacagccgc tctgcatcca cacaaatgga ggacttaatc
     5521 gtggactgca ttcttagaaa tgatctacaa agacaaataa tgtgaaatca agaaaggaca
     5581 aaatttaagt aaggggatga gggagagaga gaacgagggg caaggagaaa gcatggctcc
     5641 tgtctttttc tgcacccatc tgttcggagt gcaggtggag ctctattcac tcagctctgc
     5701 atgtgtgttt ggggggggca ggaagaaagg gagggcaaaa ggaagagtgg agagatggtg
     5761 ggggctggag ggatgggggg ttctcggtga tctctcctga aggggataat gggagagcag
     5821 cgctttgcaa tggctgccat gtagtaccct ccctgcacaa ttagccaatc agcagcaagc
     5881 tctgccagcc agaaggacac ataaaagaag aacattgcag cagaggcaca gaaggagcct
     5941 gcgaggagct gggaaataca cacacaacag cagaaccaca acaccctccc ctggacacac
     6001 cctactgggg atcactgctt ttcttttttt ctgaaccatc gcccacgcca cacggagaga
     6061 aatctctctc tcatcatcat cctgaagaaa acccccttat cctcattttc acactgctga
     6121 ggaaaaccta caatcgcacg ggctgagatt tcctggcgaa gactgtcctt tttccttttt
     6181 cttttttttt cttttccttt ggaaactgac atttgcattt ctccattcca agccacggcg
     6241 taataatatc tgcaatccag cctgaagacc tgcaaatcga aggacctaga tcactatcat
     6301 ctttgtacgt caaggatcct ggttactgta cgtataacct ttctttcttt tgctctgacc
     6361 aatatgaaac actaaaatcg attcgagcag cctctcagca tcaattacag tgcgtgaaaa
     6421 acattcaaat agaggcagca aatattacat gtgaaaatac aggctggcta aatccaagct
     6481 aatttagaaa tgtggtcaaa acgcatactg gcacgtctaa tcgcggattc agtaaacaag
     6541 attaacgatt agcccagtgt ataagtcata tgatacaggc atgcgcgaga gcatcgctac
     6601 acccgagctg gcttcatttt cggaggaaaa tcaaaacatt gctttctcct gccgtgcgaa
     6661 ccattcgtca taaaccgtaa tacgcaacat acatttatta ctacatccgt taattagcga
     6721 taattagccg ttattaacaa agagcgctga ggaattcctc tcaaatagcg gaggtgcggc
     6781 ggaggcagag gggcgtaaaa gggcacatgc gtgcctggct caaaaaagga tgctccagac
     6841 tgaaactcag agacaaaaca cgatgcctcg gaggctgaga gcccatcgag aggaaaggca
     6901 aagaaaaggg tcgagtcact cagaggggga ggggagatat tgtgcatcca ttggtttgaa
     6961 ttgaagcagg cagaataaaa atccttgcga ttatgcttcg tgagagatcg cgagagagga
     7021 aaaggctaaa tgccacgtat gcaaatggat aaatgtcatc tatttcttcg cgagtagaca
     7081 ttttgtggac aacgagagtg taaataagcg atatgagggg aatacaaagg ccttgaggga
     7141 aagcgttttg ggcgtgtctt gtcgaaaaga agacagcgat ggcacgcgct cagaaccacc
     7201 atcttcacgt agataatccg cgtgaaaaca ataacaaaac tgctcatttt aacacgagca
     7261 ggtgtttgaa accacccaaa aaagattgtc taaaatatta agattaatta atgacgttta
     7321 aatgtccagg cctgatataa atgaagcgtg tagtaagagt aatcgccttt gtttcttttc
     7381 agagatacgt tttcagagat tcagggaatt atatcttcaa taaaataatg tttgatttca
     7441 ttattttaaa gctttttaag aagtaaaaat acacattatt catttacatt ttattttatt
     7501 taaaggccta tggtcttgaa aataataagt ggaatatata ggctatatat tcgtctaagg
     7561 acagccggtg ttattttata gtgacctcag acaaaagccg agacaaaacg aggcgcttgc
     7621 ctttcatttt aatccttaat agacggtgtg atgaatgaat gaatgaatga ataaatgaat
     7681 gaatgacggg cccttcggca tgttgttccg cagtcctccg tgagcacgag tctctaccgc
     7741 agtgccaagc acataaaacc gttgagtcag ataaccttct ccaggcgcgt atacacaatt
     7801 caaggacaag cacatcacaa ctatacacaa cacaacacaa ccgcctaaac gcacgagctc
     7861 atcaatcgca atttaaaggc atgtcagtca aagaaaaagg cggcgttatt taaaaccaat
     7921 gttcaacaat atttgtatgc tgactagccc gggcgcgcac gctataagat cacaaggcct
     7981 tgttttttcc ctttattcaa aatatcatta attattacag gtcataaaac aaaacaatgc
     8041 gaattacaat gatttaaaga ctattgattt aacaataatc agttaattat agaataatat
     8101 tataatattt tacatacaaa tgaaagatga cattatttga tgacattgta attaaaatat
     8161 atgcaatata tttttacgtt tttattaatt aggctattac tattattata attatcatta
     8221 tttttattat tattattatt attaaatagt cttgcgaaat aagagtgaag gcagttgcaa
     8281 tgttcattgt tcccggagag tcccgcagcc tctggcctca acacaaagcg ctactgttta
     8341 cattaatatt catagtgctg ccatggtctc tgtagaggag gaacgggtgg ctcttttgtt
     8401 cgctagtgtc aatacgttta ttcagcaaaa agcagctcgg atttgtgaat gcccaacaag
     8461 cacacgcgca ctccaatcaa aggacgagag gataacgatt ttaaacgatt cgcaaacgtg
     8521 tttatttgta aagacgacat caagagctta atgtccactt gaaaagaaat aaatatgccc
     8581 gcggcgccaa ggatattttg aatgtagtcg ttcatggtga gccactcccg aattcagcac
     8641 tctggagagc gaccaaagag gaatagcgga atagcaacta cttgagccat ttctctccgt
     8701 ttgatggcgt tcatgattaa aaatataatc acgtgatttt ttatacatat ataaatatat
     8761 acataaagta acgctttcct tttgagaata cataaatatt tattcgcgtg ataaatcaat
     8821 gatcttatat ttatttgcac gaccgaaatt agccaaattc accaattaaa aaaaaaataa
     8881 gccaacaaaa aagaagggtt tatccgttca attttgactt gtgtcctgtt gttttacagc
     8941 tgccactgtg atcccttaag ctgcagtgag agtgtggcta atgccttttg tttaagataa
     9001 tcctgtaatc tgttaccgaa acggcctatt gacaagccgg cattcacatt tcagtcagga
     9061 ggcccgtcca gacatgtaca tttatgaatt acgaatgata aaattaagat ctgcattagg
     9121 cgtcagaaat gtcacgaaca cccattcatt tcccacagcc aataatggat catgctggga
     9181 agcgctatcc tcgcagtctc aaaattaatg ttgacatgtt gcgtagagct acagattaga
     9241 tcagccatat gctttatgtg tttacctcag cggatcttca gcaagatgtg tttattttaa
     9301 gaaaatggct tcctcgctgc ctgcacaggg gcattaagga agtgacgtga gcgtcctcac
     9361 agtcaaatta ctttatctca tgctgctttc aggcctctgc ttattttatt attattattt
     9421 taaattaggg ggaatcacgg tggcgttgtg ggtagggtag cgcgatcacc ttacagcaag
     9481 aaggtcgctg gttcgagctc tggctgggtc agttggcatt tctgtgtgga gtttaaattg
     9541 aattgaaatt gaataaacta aattggccct agtgtatgtg tgtgaatgta agtgtgcatg
     9601 ggtgtttccc agtgttgggt ttgactggaa gagcatccgc ttcgtaaaac atatgctgga
     9661 taagttggcg gttcatttca ctgtggtgac cgctgattaa taaagggact aagctgaaaa
     9721 tgaaatgaat taatacattt taaatgagct tttgctgaca tgtttatgat tacaaatagt
     9781 gaaattgtat gttaatttac gataagtgca ttgttctaaa ttcattttta tgtagcagct
     9841 ggtttttctt ccatttgtgt cattcaacag aacatttttc taaaaaagtg aacttaaatt
     9901 ttctcctccc tctgacaagg cttgatggta atttcagccg actctaatta cagactcact
     9961 aaaaggaatc ggtgcagtca tttatcttta tcagcgcgct ggtgggaagg taataggttt
    10021 tgcttatgag ttgtttgcgt gcgagtctgg gtccttgtgt aggcgatgct gatgcactgc
    10081 attaaatatt gagggagaag tcgctcaaaa atagcaaagt acaggctggg gcaggaacgt
    10141 caaagcccgc tcagtggcca cgtttcttgt ttcgtaagtc ctatttttaa tagcgataat
    10201 tcaggctcag tagaaggaac tccagcacta taagcagtct ctcgcctgtc tcggcgagtt
    10261 atgtattttg ggatgaagtg ctcttccacc tctacctctg ctgtgctggc gccagtatct
    10321 gggctgtaag atagtgtacg tgtcagtctg tgtggccgaa gagcctgtgt gttgtgtaac
    10381 tcactacatt cgtcgctgtg gatggctgac ttgccatggg gaggtttact tcagttcacg
    10441 aatcacattc gccacgatct tgtcactttg tgtcaagact cgctctctgt gtgtcaacag
    10501 ctctgctgtt tgaggttgaa attctgaact tgagatatat cccaggatat attagggcta
    10561 ctgccaacgg aaaaagcaaa tggtgccatg gccatttgat tgatcaaaca aacacccttt
    10621 tgcttgggtg acagtgaaat gtcagatcca tgtttgtact gctttaataa cttgcctctc
    10681 tgagctttct ccttgtcagt gtcaatttgc ctgagatata aattccggtt gccccgaaag
    10741 cttattttat taaaaaaaag ctaacatgca ctcatacaac gtgcgcacat acatgtgagg
    10801 cttgctaaaa gtcaagaagc aagccaatag caagagtcgt cagactgctg ttacctgggc
    10861 aacctggtta gaggtaaggc tcaaggcaag aataaatcat tccactgaag agacacagcg
    10921 ttacagcgag atgagagcaa gaggcgatag tgaagggaga acaaaagaac agcctaccca
    10981 cactagtgca gatctacctg tttgagctac tgattgttca gtttgattag atatcagaca
    11041 attctgctat ctccaattgc attgctgcat gaattcctag aaatcatttt aatcaccaaa
    11101 aactagtagg gacacttgtg gcgtatatga ttctgcctgt tgattgtggg gatccaacag
    11161 aaagatcatg aatgcattgt taattaagtc ggtgagacac cctgttccac cctaggggcc
    11221 gttctgggga aagagtgctt tcagtcagct aaagatgact aatatgtgaa catatattac
    11281 tacacatgcc aatttgtaca ttctggatag ttaccagccc tgggaaaaca tcgaaaaaaa
    11341 taaatcaaca tagataaaat tggcaaatcc cttctggtac gatgcagagt gcttgctctg
    11401 agaacttgtg cattgtgaca gaaagcgacc gttaaaaact gaaagtagca tctacaacct
    11461 gcatacatct gttctttgaa aatcccctgt aggccaaatt aagcatcacg ctgtgctcca
    11521 cagcaacacg gatgaaccga aaacccagac acaaacgcac acacttttca cataaacaag
    11581 gtttagttat gctagctatg ctttttcgtc ctccctttgt catcagcggc gagtgacgta
    11641 acacagtttg accctggaca gcgtaaaggg ggggttggga atgagtgaaa ggactgtgac
    11701 atctgcaggg ccaggggctg atggagagag ctgaagtgag ggaggtcaga ggaggaggaa
    11761 gggtgggata ttgtcctcct caggccctat ccctctcaga cgcattgatt tttaacctct
    11821 cagcgagaat gccaagcatt acacccctca aattatggtt cctcacccaa cctgttatat
    11881 tttccacctg cagataatta gcaccattga aactcgaggc cgcaaacatg gagacagaca
    11941 cactcctgct atgggtactg ctgctctggg ttccaggttc cactggtgac acaagtttgt
    12001 acaaaaaagt tggcaccggc tccctgcagc cggacgcggg caacgcgagc tggaacggga
    12061 ccgaggcgcc ggggggcggc gcccgggcca ccccttactc cctgcaggtg acgctgacgc
    12121 tggtgtgcct ggccggcctg ctcatgctgc tcaccgtgtt cggcaacgtg ctcgtcatca
    12181 tcgccgtgtt cacgagccgc gcgctcaagg cgccccaaaa cctcttcctg gtgtctctgg
    12241 cctcggccga catcctggtg gccacgctcg tcatcccttt ctcgctggcc aacgaggtca
    12301 tgggctactg gtacttcggc aaggcttggt gcgagatcta cctggcgctc gacgtgctct
    12361 tctgcacgtc gtccatcgtg cacctgtgcg ccatcagcct ggaccgctac tggtccatca
    12421 cacaggccaa agagtacaac ctgaagcgca cgccgcgccg catcaaggcc atcatcatca
    12481 ccgtgtgggt catctcggcc gtcatctcct tcccgccgct catctccatc gagaagaagg
    12541 gcggcggcgg cggcccgcag ccggccgagc cgcgctgcga gatcaacgac cagaagtggt
    12601 acgtcatctc gtcgtgcatc ggctccttct tcgctccctg cctcatcatg atcctggtct
    12661 acgtgcgcat ctaccagatc gccaagcgtc gcacccgcgt gccacccagc cgccggggtc
    12721 cggacgccgt cgccgcgccg ccggggggca ccgagcgcag gcccaacggt ctgggccccg
    12781 agcgcagcgc gggcccgggg ggcgcagagg ccgaaccgct gcccacccag ctcaacggcg
    12841 cccctggcga gcccgcgccg gccgggccgc gcgacaccga cgcgctggac ctggaggagg
    12901 gaggaaacgt ctatatcaag gccgacaagc agaagaacgg catcaaggcg aacttccaca
    12961 tccgccacaa catcgaggac ggcggcgtgc agctcgccta ccactaccag cagaacaccc
    13021 ccatcggcga cggccccgtg ctgctgcccg acaaccacta cctgagcgtg cagtccaaac
    13081 tttcgaaaga ccccaacgag aagcgcgatc acatggtcct gctggagttc gtgaccgccg
    13141 ccgggatcac tctcggcatg gacgagctgt acaagggcgg taccggaggg agcatggtga
    13201 gaaagggcga ggagctgttc accggggtgg tgcccatcct ggtcgagctg gacggcgacg
    13261 taaacggcca caagttcagc gtgtccggcg agggtgaggg cgatgccacc gagggcaagc
    13321 tgaccctgaa gttcatctgc accaccggca agctgcccgt gccctggccc accctcgtga
    13381 ccaccctgac ctacggcgtg cagtgcttca gccgctaccc cgaccacatg aagcagcacg
    13441 acttcttcaa gtccgccatg cccgaaggct acatccagga gcgcaccatc ttcttcaagg
    13501 acgacggcaa ctacaagacc cgcgccgagg tgaagttcga gggcgacacc ctggtgaacc
    13561 gcatcgagct gaagggcatc gacttcaagg aggacggcaa catcctgggg cacaagctgg
    13621 agtacaacac cggagcagca gcacgctggc gcgggcggca gaaccgcgag aagcgcttca
    13681 ttttcgtgct ggccgtggtc atcggagtgt tcgtggtgtg ctggttcccc ttcttcttca
    13741 cctacacgct cacggccgtc gggtgctccg tgccacgcac gctcttcaaa ttcttcttct
    13801 ggttcggcta ctgcaacagc tcgttgaacc cggtcatcta caccatcttc aaccacgatt
    13861 tccgccgcgc cttcaagaag atcctctgtc ggggggacag gaagcggatc gtgttgtgag
    13921 cggccgcgac tctagatcat aatcagccat accacatttg tagaggtttt acttgcttta
    13981 aaaaacctcc cacacctccc cctgaacctg aaacataaaa tgaatgcaat tgttgttgtt
    14041 aacttgttta ttgcagctta taatggttac aaataaagca atagcatcac aaatttcaca
    14101 aataaagcat ttttttcact gcattctagt tgtggtttgt ccaaactcat caatgtatct
    14161 taaggcgtaa attgtaagcg ttaatatttt gttaaaattc gcgttaaatt tttgttaaat
    14221 cagctcattt tttaaccaat aggccgaaat cggcaaaatc ccttataaat caaaagaata
    14281 gaccgagata gggttgagtg ttgttccagt ttggaacaag agtccactat taaagaacgt
    14341 ggactccaac gtcaaagggc gaaaaaccgt ctatcagggc gatggcccac tacgtgaacc
    14401 atcaccctaa tcaagttttt tggggtcgag gtgccgtaaa gcactaaatc ggaaccctaa
    14461 agggagcccc cgatttagag cttgacgggg aaagccggcg aacgtggcga gaaaggaagg
    14521 gaagaaagcg aaaggagcgg gcgctagggc gctggcaagt gtagcggtca cgctgcgcgt
    14581 aaccaccaca cccgccgcgc ttaatgcgcc gctacagggc gcgtcaggtg gcacttttcg
    14641 gggaaatgtg cgcggaaccc ctatttgttt atttttctaa atacattcaa atatgtatcc
    14701 gctcatgaga caataaccct gataaatgct tcaataatat tgaaaaagga agagtcctga
    14761 ggcggaaaga accagctgtg gaatgtgtgt cagttagggt gtggaaagtc cccaggctcc
    14821 ccagcaggca gaagtatgca aagcatgcat ctcaattagt cagcaaccag gtgtggaaag
    14881 tccccaggct ccccagcagg cagaagtatg caaagcatgc atctcaatta gtcagcaacc
    14941 atagtcccgc ccctaactcc gcccatcccg cccctaactc cgcccagttc cgcccattct
    15001 ccgccccatg gctgactaat tttttttatt tatgcagagg ccgaggccgc ctcggcctct
    15061 gagctattcc agaagtagtg aggaggcttt tttggaggaa attaaactgg gcatcagcgc
    15121 aattcaattg gtttggtaat agcaagggaa aatagaatga agtgatctcc aaaaaataag
    15181 tactttttga ctgtaaataa aattgtaagg agtaaaaagt actttttttt ctaaaaaaat
    15241 gtaattaagt aaaagtaaaa gtattgattt ttaattgtac tcaagtaaag taaaaatccc
    15301 caaaaataat acttaagtac agtaatcaag taaaattact caagtacttt acacctctgg
    15361 gcccaattcg ccc
//
