LOCUS       sequence-411125-        9190 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Dual-luciferase plasmid to measure enhancer activity in plants.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9190)
  AUTHORS   Jores T, Tonnies J, Mueth NA, Romanowski A, Fields S, Cuperus JT, 
            Queitsch C
  TITLE     Plant enhancers exhibit both cooperative and additive interactions 
            among their functional elements.
  JOURNAL   Plant Cell. 2024 Mar 21:koae088. doi: 10.1093/plcell/koae088.
  PUBMED    38513612
REFERENCE   2  (bases 1 to 9190)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Plant Cell.
            2024 Mar 21:koae088. doi: 10.1093/plcell/koae088."
COMMENT     Sequence Label: pDL
FEATURES             Location/Qualifiers
     source          1..9190
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     1..17
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     27..46
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        27..45
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     70..86
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     primer_bind     71..87
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        151..197
                     /label=minimal CaMV 35S promoter
                     /note="minimal 35S promoter from cauliflower mosaic virus"
     CDS             225..740
                     /codon_start=1
                     /product="NanoLuc(R) luciferase"
                     /label=Nluc
                     /note="human codon-optimized"
                     /translation="MVFTLEDFVGDWRQTAGYNLDQVLEQGGVSSLFQNLGVSVTPIQR
                     IVLSGENGLKIDIHVIIPYEGLSGDQMGQIEKIFKVVYPVDDHHFKVILHYGTLVIDGV
                     TPNMIDYFGRPYEGIAVFDGKKITVTGTLWNGNKIIDERLINPDGSLLFRVTINGVTGW
                     RLCERILA"
     primer_bind     complement(1061..1081)
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        complement(1061..1079)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(1100..1116)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(1100..1116)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(1113..1135)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    1124..1140
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(1148..1154,1155..1172,1173..1178))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    1193..1214
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(1331..1348)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     misc_feature    1453..1477
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     rep_origin      complement(1568..2156)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(1648..1667)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(2327..3115)
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNGTLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEDRLASRADQLEEFVHYVKGEITKVVGK"
     rep_origin      3406..3841
                     /label=pSa ori
                     /note="origin of replication from bacterial plasmid pSa"
     misc_feature    3969..3991
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA 
                     (truncated)"
     CDS             complement(4209..5861)
                     /codon_start=1
                     /gene="luc+"
                     /product="firefly luciferase"
                     /label=luciferase
                     /note="enhanced luc+ version of the luciferase gene"
                     /translation="MEDAKNIKKGPAPFYPLEDGTAGEQLHKAMKRYALVPGTIAFTDA
                     HIEVDITYAEYFEMSVRLAEAMKRYGLNTNHRIVVCSENSLQFFMPVLGALFIGVAVAP
                     ANDIYNERELLNSMGISQPTVVFVSKKGLQKILNVQKKLPIIQKIIIMDSKTDYQGFQS
                     MYTFVTSHLPPGFNEYDFVPESFDRDKTIALIMNSSGSTGLPKGVALPHRTACVRFSHA
                     RDPIFGNQIIPDTAILSVVPFHHGFGMFTTLGYLICGFRVVLMYRFEEELFLRSLQDYK
                     IQSALLVPTLFSFFAKSTLIDKYDLSNLHEIASGGAPLSKEVGEAVAKRFHLPGIRQGY
                     GLTETTSAILITPEGDDKPGAVGKVVPFFEAKVVDLDTGKTLGVNQRGELCVRGPMIMS
                     GYVNNPEATNALIDKDGWLHSGDIAYWDEDEHFFIVDRLKSLIKYKGYQVAPAELESIL
                     LQHPNIFDAGVAGLPDDDAGELPAAVVVLEHGKTMTEKEIVDYVASQVTTAKKLRGGVV
                     FVDEVPKGLTGKLDARKIREILIKAKKGGKIAV"
     primer_bind     5777..5795
                     /label=LucNrev
                     /note="Firefly luciferase, reverse primer"
     terminator      complement(7900..8152)
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     CDS             complement(8172..8723)
                     /codon_start=1
                     /gene="Streptomyces hygroscopicus bar"
                     /product="phosphinothricin acetyltransferase"
                     /label=BlpR
                     /note="confers resistance to bialophos or phosphinothricin"
                     /translation="MSPERRPADIRRATEADMPAVCTIVNHYIETSTVNFRTEPQEPQE
                     WTDDLVRLRERYPWLVAEVDGEVAGIAYAGPWKARNAYDWTAESTVYVSPRHQRTGLGS
                     TLYTHLLKSLEAQGFKSVVAVIGLPNDPSVRMHEALGYAPRGMLRAAGFKHGNWHDVGF
                     WQLDFSLPVPPRPVLPVTEI"
     promoter        complement(8765..8944)
                     /label=NOS promoter
                     /note="nopaline synthase promoter"
     primer_bind     join(9176..9190,1..8)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     join(9190,1..17)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
ORIGIN
        1 gtaaaacgac ggccagtgag cgcgcgtaat acgactcact atagggcgaa ttgggtaccg
       61 ggccccccct cgaggtcgac ggtatcgata agcttgatat cgaattccac tccgagacca
      121 caaggcgcgc ctagtggtct ccaggagctg gcaagaccct tcctctatat aaggaagttc
      181 atttcatttg gagaggacac gctggaattc tagtatacta aaccatggtc ttcacactcg
      241 aagatttcgt tggggactgg cgacagacag ccggctacaa cctggaccaa gtccttgaac
      301 agggaggtgt gtccagtttg tttcagaatc tcggggtgtc cgtaactccg atccaaagga
      361 ttgtcctgag cggtgaaaat gggctgaaga tcgacatcca tgtcatcatc ccgtatgaag
      421 gtctgagcgg cgaccaaatg ggccagatcg aaaaaatttt taaggtggtg taccctgtgg
      481 atgatcatca ctttaaggtg atcctgcact atggcacact ggtaatcgac ggggttacgc
      541 cgaacatgat cgactatttc ggacggccgt atgaaggcat cgccgtgttc gacggcaaaa
      601 agatcactgt aacagggacc ctgtggaacg gcaacaaaat tatcgacgag cgcctgatca
      661 accccgacgg ctccctgctg ttccgagtaa ccatcaacgg agtgaccggc tggcggctgt
      721 gcgaacgcat tctggcgtaa agcggccgca aattgcccag aattaattcc ccaactgcac
      781 agaacaaatt tccctttgct tttgtgtaga cctcaaaact ttatccccca tctttgatct
      841 tatcccttgt ttttctgctt ttttcttctt tcttgggttt tgatttccgg acttaacgtt
      901 tgttttccgg tttgcgagac atattctatc ggattctcaa ctgtctgatg aaataaatat
      961 gtaatgttct ataagtcttt caatttgatg ggatgttcca cttctgtata ggagccaatt
     1021 gattgacaac atgcatcaat cggagctcca gcttttgttc cctttagtga gggttaattg
     1081 cgcgcttggc gtaatcatgg tcatagctgt ttcctgtgtg aaattgttat ccgctcacaa
     1141 ttccacacaa catacgagcc ggaagcataa agtgtaaagc ctggggtgcc taatgagtga
     1201 gctaactcac attaattgcg ttgcgctcac tgcccgcttt ccagtcggga aacctgtcgt
     1261 gccagctgca ttaatgaatc ggccaacgct cggggagagg cggtttgcgt attgggcgct
     1321 cttccgcttc ctcgctcact gactcgctgc gctcggtcgt tcggctgcgg cgagcggtat
     1381 cagctcactc aaaggcggta atacggttat ccacagaatc aggggataac gcaggaaaga
     1441 acatgaaggc cttgacagga tatattggcg ggtaaactaa gtcgctgtat gtgtttgttt
     1501 gagatctcat gtgagcaaaa ggccagcaaa aggccaggaa ccgtaaaaag gccgcgttgc
     1561 tggcgttttt ccataggctc cgcccccctg acgagcatca caaaaatcga cgctcaagtc
     1621 agaggtggcg aaacccgaca ggactataaa gataccaggc gtttccccct ggaagctccc
     1681 tcgtgcgctc tcctgttccg accctgccgc ttaccggata cctgtccgcc tttctccctt
     1741 cgggaagcgt ggcgctttct catagctcac gctgtaggta tctcagttcg gtgtaggtcg
     1801 ttcgctccaa gctgggctgt gtgcacgaac cccccgttca gcccgaccgc tgcgccttat
     1861 ccggtaacta tcgtcttgag tccaacccgg taagacacga cttatcgcca ctggcagcag
     1921 ccactggtaa caggattagc agagcgaggt atgtaggcgg tgctacagag ttcttgaagt
     1981 ggtggcctaa ctacggctac actagaagaa cagtatttgg tatctgcgct ctgctgaagc
     2041 cagttacctt cggaagaaga gttggtagct cttgatccgg caaacaaacc accgctggta
     2101 gcggtggttt ttttgtttgc aagcagcaga ttacgcgcag aaaaaaagga tctcaagaag
     2161 atcctttgat cttttctacg gggtctgacg ctcagtggaa cgaaaactca cgttaaggga
     2221 ttttggtcat tagattatca aaaaggatct tcacctagat ccttttaaat taaaaatgaa
     2281 gttttaaatc aatctaaagt atatatgtgt aacattggtc tagtgattat ttgccgacta
     2341 ccttggtgat ctcgcctttc acgtagtgaa caaattcttc caactgatct gcgcgcgagg
     2401 ccaagcgatc ttcttgtcca agataagcct gcctagcttc aagtatgacg ggctgatact
     2461 gggccggcag gcgctccatt gcccagtcgg cagcgacatc cttcggcgcg attttgccgg
     2521 ttactgcgct gtaccaaatg cgggacaacg taagcactac atttcgctca tcgccagccc
     2581 agtcgggcgg cgagttccat agcgttaagg ttccatttag cgcctcaaat agatcctgtt
     2641 caggaaccgg atcaaagagt tcctccgccg ctggacctac caaggcaacg ctatgttctc
     2701 ttgcttttgt cagcaagata gccagatcaa tgtcgatcgt ggctggctcg aagatacctg
     2761 caagaatgtc attgcgctgc cattctccaa attgcagttc gcgcttagct ggataacgcc
     2821 acggaatgat gtcgtcgtgc acaacaatgg tgacttctac agcgcggaga atctcgctct
     2881 ctccagggga agccgaagtt tccaaaaggt cgttgatcaa agctcgccgc gttgtttcat
     2941 caagccttac ggtcaccgta accagcaaat caatatcact gtgtggcttc aggccgccat
     3001 ccactgcgga gccgtacaaa tgtacggcca gcaacgtcgg ttcgagatgg cgctcgatga
     3061 cgccaactac ctctgatagt tgagtcgata cttcggcgat caccgcttcc ctcataacac
     3121 cccttgtatt actgtttatg taagcagaca gttttattgt tcatgatgat atatttttat
     3181 cttgtgcaat gtaacatcag agattttgag acacaacgtg gctttgttga ataaatcgaa
     3241 cttttgctga gttgaaggat cagatcacgc atcttcccga caacgcagac cgttccgtgg
     3301 caaagcaaaa gttcaaaatc accaactggt ccacctacaa caaagctctc atcaaccgtg
     3361 gctccctcac tttctggctg gatgatgggg cgattcaggc gatccccatc caacagcccg
     3421 ccgtcgagcg ggctttttta tccccggaag cctgtggata gagggtagtt atccacgtga
     3481 aaccgctaat gccccgcaaa gccttgattc acggggcttt ccggcccgct ccaaaaacta
     3541 tccacgtgaa atcgctaatc agggtacgtg aaatcgctaa tcggagtacg tgaaatcgct
     3601 aataaggtca cgtgaaatcg ctaatcaaaa aggcacgtga gaacgctaat agccctttca
     3661 gatcaacagc ttgcaaacac ccctcgctcc ggcaagtagt tacagcaagt agtatgttca
     3721 attagctttt caattatgaa tatatatatc aattattggt cgcccttggc ttgtggacaa
     3781 tgcgctacgc gcaccggctc cgcccgtgga caaccgcaag cggttgccca ccgtcgagcg
     3841 cctttgccca caacccggcg gccggccgca acagatcgtt ttataaattt tttttttttg
     3901 aaaaagaaaa agcccgaaag gcggcaacct ctcgggcttc tggatttccg atccccggaa
     3961 ttagatcttg gcaggatata ttgtggtgta acgttatcag cttatctgga ttttagtact
     4021 ggattttggt tttaggaatt agaaatttta ttgatagaag tattttacaa atacaaatac
     4081 atactaaggg tttcttatat gctcaacaca tgagcgaaac cctataggaa ccctaattcc
     4141 cttatctggg aactactcac acattattat ggagaaactc gagcttgtcg atcgactcta
     4201 gctagagctt acacggcgat ctttccgccc ttcttggcct ttatgaggat ctctctgatt
     4261 tttcttgcgt cgagttttcc ggtaagacct ttcggtactt cgtccacaaa cacaactcct
     4321 ccgcgcaact ttttcgcggt tgttacttga ctggcgacgt aatccacgat ctctttttcc
     4381 gtcatcgtct ttccgtgctc caaaacaaca acggcggcgg gaagttcacc ggcgtcatcg
     4441 tcgggaagac ctgcgacacc tgcgtcgaag atgttggggt gttggagcaa gatggattcc
     4501 aattcagcgg gagccacctg atagcctttg tacttaatca gagacttcag gcggtcaacg
     4561 atgaagaagt gttcgtcttc gtcccagtaa gctatgtctc cagaatgtag ccatccatcc
     4621 ttgtcaatca aggcgttggt cgcttccgga ttgtttacat aaccggacat aatcatagga
     4681 cctctcacac acagttcgcc tctttgatta acgcccagcg ttttcccggt atccagatcc
     4741 acaaccttcg cttcaaaaaa tggaacaact ttaccgaccg cgcccggttt atcatccccc
     4801 tcgggtgtaa tcagaatagc tgatgtagtc tcagtgagcc catatccttg cctgatacct
     4861 ggcagatgga acctcttggc aaccgcttcc ccgacttcct tagagagggg agcgccacca
     4921 gaagcaattt cgtgtaaatt agataaatcg tatttgtcaa tcagagtgct tttggcgaag
     4981 aaggagaata gggttggcac cagcagcgca ctttgaatct tgtaatcctg aaggctcctc
     5041 agaaacagct cttcttcaaa tctatacatt aagacgactc gaaatccaca tatcaaatat
     5101 ccgagtgtag taaacattcc aaaaccgtga tggaatggaa caacacttaa aatcgcagta
     5161 tccggaatga tttgattgcc aaaaatagga tctctggcat gcgagaatct cacgcaggca
     5221 gttctatgag gcagagcgac acctttaggc agaccagtag atccagagga gttcatgatc
     5281 agtgcaattg tcttgtccct atcgaaggac tctggcacaa aatcgtattc attaaaaccg
     5341 ggaggtagat gagatgtgac gaacgtgtac atcgactgaa atccctggta atccgtttta
     5401 gaatccatga taataatttt ttggatgatt gggagctttt tttgcacgtt caaaattttt
     5461 tgcaacccct ttttggaaac gaacaccacg gtaggctgcg aaatgcccat actgttgagc
     5521 aattcacgtt cattataaat gtcgttcgcg ggcgcaactg caactccgat aaataacgcg
     5581 cccaacaccg gcataaagaa ttgaagagag ttttcactgc atacgacgat tctgtgattt
     5641 gtattcagcc catatcgttt catagcttct gccaaccgaa cggacatttc gaagtactca
     5701 gcgtaagtga tgtccacctc gatatgtgca tctgtaaaag caattgttcc aggaaccagg
     5761 gcgtatctct tcatagcctt atgcagttgc tctccagcgg ttccatcttc cagcggatag
     5821 aatggcgccg ggcctttctt tatgtttttg gcgtcttcca tctgttaatc agaaaaactc
     5881 agattaatcg acaaattcga tcgcacaaac tagaaactaa caccagatct agatagaaat
     5941 cacaaatcga agagtaatta ttcgacaaaa ctcaaattat ttgaacaaat cggatgatat
     6001 ctatgaaacc ctaatcgaga attaagatga tatctaacga tcaaacccag aaaatcgtct
     6061 tcgatctaag attaacagaa tctaaaccaa agaacatata cgaaattggg atcgaacgaa
     6121 aacaaaatcg aagattttga gagaataagg aacacagaaa tttaccttga tcacggtaga
     6181 gagaattgag agaaagtttt taagattttg agaaattgaa atctgaattg tgaagaagaa
     6241 gagctctttg ggtattgttt tatagaagaa gaagaagaaa agacgaggac gactaggtca
     6301 cgagaaagct aaggcggtga agcaatagct aataataaaa tgacacgtgt attgagcgtt
     6361 gtttacacgc aaagttgttt ttggctaatt gccttatttt taggttgagg aaaagtattt
     6421 gtgctttgag ttgataaaca cgactcgtgt gtgccggctg caaccacttt gacgccgttt
     6481 attactgact cgtcgacaac cacaatttct aacggtcgtc ataagatcca gccgttgaga
     6541 tttaacgatc gttacgattt atattttttt agcattatcg ttttattttt taaatatacg
     6601 gtggagctga aaattggcaa taattgaacc gtgggtccca ctgcattgaa gcgtatttcg
     6661 tattttctag aattcttcgt gctttatttc ttttcctttt tgtttttttt tgccatttat
     6721 ctaatgcaag tgggcttata aaatcagtga atttcttgga aaagtaactt ctttatcgta
     6781 taacatattg tgaaattatc catttctttt aattttttag tgttattgga tatttttgta
     6841 tgattattga tttgcatagg ataatgactt ttgtatcaag ttggtgaaca agtctcgtta
     6901 aaaaaggcaa gtggtttggt gactcgattt attcttgtta tttaattcat atatcaatgg
     6961 atcttatttg gggcctggtc catatttaac actcgtgttc agtccaatga ccaataatat
     7021 tttttcatta ataacaatgt aacaagaatg atacacaaaa cattctttga ataagttcgc
     7081 tatgaagaag ggaacttatc cggtcctaga tcatcagttc atacaaacct ccatagagtt
     7141 caacatctta aacaagaata tcctgatctg aagaatgtgg aggctttagt cccttggata
     7201 cttgggaggc tgtggaagaa cagaaacgag ctggtgctta aagggaggga atttggaacc
     7261 aatgaggtat tagtaaggac acaagaagat gcagatgagt ggattagaag gaaagaggct
     7321 cagaatgtaa ggaaagcacc aaccacaaca ccgtcgggac agacgagaaa catgggaagc
     7381 tccaccacaa tcttgggtta agtgcaactt tgatggggca tggccaacag aaggattaaa
     7441 atgtggctta gggtgggtgc ttcgcgatca tacagggaag gtgttatggt taggtgcaag
     7501 agctgtggta aaagtaagaa gcgtgctgga agtagaagtg gaggctctta gatgggctgt
     7561 gctgtcatta tcccgattca attataggaa gatcattttt gaggtggatt ctcagcaact
     7621 tgtgtctttg gttacatgaa agttatgctt gtcaagtctc aatccaatta tccaagacat
     7681 aaagtatcta cttagcaagt ttgaggattt tatgcttgtg catacaagcc gagaaggaaa
     7741 tggagtggca gatagaatag ctaaggaatc tctttctttt gagaattatg atctaaagtt
     7801 gtattctatt gtaccaattt gggttaaaag ctctgttgag ctagactgtg tatccataaa
     7861 tgggtgaatg gaaagtttgt tgttgagcgc atgccggtcg atctagtaac atagatgaca
     7921 ccgcgcgcga taatttatcc tagtttgcgc gctatatttt gttttctatc gcgtattaaa
     7981 tgtataattg cgggactcta atcataaaaa cccatctcat aaataacgtc atgcattaca
     8041 tgttaattat tacatgctta acgtaattca acagaaatta tatgataatc atcgcaagac
     8101 cggcaacagg attcaatctt aagaaacttt attgccaaat gttttaacga tctgcttgac
     8161 tctaggggtc atcagatttc ggtgacgggc aggaccggac ggggcggcac cggcaggctg
     8221 aagtccagct gccagaaacc cacgtcatgc cagttcccgt gcttgaagcc ggccgcccgc
     8281 agcatgccgc ggggggcata tccgagcgcc tcgtgcatgc gcacgctcgg gtcgttgggc
     8341 agcccgatga cagcgaccac gctcttgaag ccctgtgcct ccagggactt cagcaggtgg
     8401 gtgtagagcg tggagcccag tcccgtccgc tggtggcggg gggagacgta cacggtggac
     8461 tcggccgtcc agtcgtaggc gttgcgtgcc ttccagggac ccgcgtaggc gatgccggcg
     8521 acctcgccgt ccacctcggc gacgagccag ggatagcgct cccgcagacg gacgaggtcg
     8581 tccgtccact cctgcggttc ctgcggctcg gtacggaagt tgaccgtgct tgtctcgatg
     8641 tagtggttga cgatggtgca gaccgccggc atgtccgcct cggtggcacg gcggatgtcg
     8701 gccgggcgtc gttctgggct catggtagat cccctcgatc gagttgagag tgaatatgag
     8761 actctaattg gataccgagg ggaatttatg gaacgtcagt ggagcatttt tgacaagaaa
     8821 tatttgctag cttatagtga ccttaggcga cttttgaacg cgcaataatg gtttctgacg
     8881 tatgtgctta gctcattaaa ctccagaaac ccgcggctca gtggctcctt caacgttgcg
     8941 gttctgtcag ttccaaacgt aaaacggctt gtcccgcgtc atcggcgggg gtcataacgt
     9001 gactccctta attctccgct catgatcgat aacattaacg cttacaattt ccattcgcca
     9061 ttcaggctgc gcaactgttg ggaagggcga tcggtgcggg cctcttcgct attacgccag
     9121 ctggcgaaag ggggatgtgc tgcaaggcga ttaagttggg taacgccagg gttttcccag
     9181 tcacgacgtt
//
