LOCUS       sequence-411128-        9743 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  GPCR/G protein BRET biosensor.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9743)
  AUTHORS   Janicot R, Maziarz M, Park JC, Zhao J, Luebbers A, Green E, 
            Philibert CE, Zhang H, Layne MD, Wu JC, Garcia-Marcos M
  TITLE     Direct interrogation of context-dependent GPCR activity with a 
            universal biosensor platform.
  JOURNAL   Cell. 2024 Feb 16:S0092-8674(24)00065-5. doi: 
            10.1016/j.cell.2024.01.028.
  PUBMED    38412860
REFERENCE   2  (bases 1 to 9743)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell. 2024 
            Feb 16:S0092-8674(24)00065-5. doi: 10.1016/j.cell.2024.01.028."
COMMENT     Sequence Label: pLVX-hSyn-Galpha-i1 ONE-GO
FEATURES             Location/Qualifiers
     source          1..9743
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    2..117
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1 (lacking the first T)"
     CDS             complement(380..424)
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    609..842
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     misc_feature    1339..1464
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     LTR             1511..2144
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     polyA_signal    2312..2446
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(2336..2355)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     2390..2409
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     promoter        2494..2598
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(2599..2667,2668..3459)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(2817..2836)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      3630..4218
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     4119..4138
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     4372..4389
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    4506..4527
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(4542..4547,4548..4565,4566..4572)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    4580..4596
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     4585..4607
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     4604..4620
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     4604..4620
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     LTR             4748..5381
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     misc_feature    5588..6176
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     CDS             5694..5705
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     6103..6123
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(6190..6705)
                     /codon_start=1
                     /product="NanoLuc(R) luciferase"
                     /label=Nluc
                     /note="human codon-optimized"
                     /translation="MVFTLEDFVGDWRQTAGYNLDQVLEQGGVSSLFQNLGVSVTPIQR
                     IVLSGENGLKIDIHVIIPYEGLSGDQMGQIEKIFKVVYPVDDHHFKVILHYGTLVIDGV
                     TPNMIDYFGRPYEGIAVFDGKKITVTGTLWNGNKIIDERLINPDGSLLFRVTINGVTGW
                     RLCERILA"
     CDS             complement(6811..6831)
                     /codon_start=1
                     /product="N-myristoylation signal from Src kinase (Pellman 
                     et al., 1985; Kaplan et al., 1988)"
                     /label=myr
                     /translation="MGSSKSK"
     misc_feature    join(6842..6850,6851..6853,6854..7428)
                     /label=IRES2
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(7015..7034)
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     primer_bind     7244..7261
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     CDS             complement(join(8144..8854,8855..8857,8858..8860))
                     /codon_start=1
                     /product="enhanced variant of YFP (Heikal et al., 2001)"
                     /label=Citrine
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTFGYGLMCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSYQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(8186..8207)
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     primer_bind     8535..8554
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     8794..8815
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     promoter        complement(9256..9703)
                     /label=hSyn promoter
                     /note="human synapsin I promoter; confers neuron-specific 
                     expression (Kugler et al., 2003)"
ORIGIN
        1 aaaattttga atttttgtaa tttgtttttg taattcttta gtttgtatgt ctgttgctat
       61 tatgtctact attctttccc ctgcactgta ccccccaatc cccccttttc ttttaaaggc
      121 gataccgtcg agatccgttc actaatcgaa tggatctgtc tctgtctctc tctccacctt
      181 cttcttctat tccttcgggc ctgtcgggtc ccctcggggt tgggaggtgg gtctgaaacg
      241 ataatggtga atatccctgc ctaactctat tcactataga aagtacagca aaaactattc
      301 ttaaacctac caagcctcct actatcatta tgaataattt tatataccac agccaatttg
      361 ttatgttaaa ccaattccac aaacttgccc atttatctaa ttccaataat tcttgttcat
      421 tcttttcttg ctggttttgc gattcttcaa ttaaggagtg tattaagctt gtgtaattgt
      481 taatttctct gtcccactcc atccaggtcg tgtgattcca aatctgttcc agagatttat
      541 tactccaact agcattccaa ggcacagcag tggtgcaaat gagttttcca gagcaacccc
      601 aaatccccag gagctgttga tcctttaggt atctttccac agccaggatt cttgcctgga
      661 gctgcttgat gccccagact gtgagttgca acagatgctg ttgcgcctca atagccctca
      721 gcaaattgtt ctgctgctgc actataccag acaataattg tctggcctgt accgtcagcg
      781 tcattgacgc tgcgcccata gtgcttcctg ctgctcccaa gaacccaagg aacaaagctc
      841 ctattcccac tgctcttttt tctctctgca ccactcttct ctttgccttg gtgggtgcta
      901 ctcctaatgg ttcaattttt actactttat atttatataa ttcacttctc caattgtccc
      961 tcatatctcc tcctccaggt ctgaagatca gcggccggcc gcttgctgtg cggtggtctt
     1021 acttttgttt tgctcttcct ctatcttgtc taaagcttcc ttggtgtctt ttatctctat
     1081 cctttgatgc acacaataga gggttgctac tgtattatat aatgatctaa gttcttctga
     1141 tcctgtctga agggatggtt gtagctgtcc cagtatttgt ctacagcctt ctgatgtttc
     1201 taacaggcca ggattaactg cgaatcgttc tagctccctg cttgcccata ctatatgttt
     1261 taatttatat tttttctttc cccctggcct taaccgaatt ttttcccatc gcgatctaat
     1321 tctcccccgc ttaatactga cgctctcgca cccatctctc tccttctagc ctccgctagt
     1381 caaaattttt ggcgtactca ccagtcgccg cccctcgcct cttgccgtgc gcgcttcagc
     1441 aagccgagtc ctgcgtcgag agagctcctc tggtttccct ttcgctttca agtccctgtt
     1501 cgggcgccac tgctagagat tttccacact gactaaaagg gtctgaggga tctctagtta
     1561 ccagagtcac acaacagacg ggcacacact acttgaagca ctcaaggcaa gctttattga
     1621 ggcttaagca gtgggttccc tagttagcca gagagctccc aggctcagat ctggtctaac
     1681 cagagagacc cagtacaggc aaaaagcagc tgcttatatg caggatctga gggctcgcca
     1741 ctccccagtc ccgcccaggc cacgcctccc tggaaagtcc ccagcggaaa gtcccttgta
     1801 gcaagctcga tatcagcagt tcttgaagta ctccggatgc agctctcggg ccacgtgatg
     1861 aaatgctagg cggctgtcaa acctccactc taacacttct ctctccgggt catccatccc
     1921 atgcaggctc acagggtgta acaagctggt gttctctcct ttattggcct cttctacctt
     1981 atctggctca actggtacta gcttgtagca ccatccaaag gtcagtggat atctgacccc
     2041 tggccctggt gtgtagttct gctaatcagg gaagtagcct tgtgtgtggt agatccacag
     2101 atcaaggata tcttgtcttc tttgggagtg aattagccct tccaactact aagtttgtag
     2161 tacatattta acaaatacaa tttctttaaa atgaaaataa ttcagaggaa tcacaggttt
     2221 agagtaaatg aaaccacagg taattggcag tggtaatagg gtatggggtg ggaagtttgg
     2281 gatgattttg gttagcttga gttatccagt tgatccagac atgataagat acattgatga
     2341 gtttggacaa accacaacta gaatgcagtg aaaaaaatgc tttatttgtg aaatttgtga
     2401 tgctattgct ttatttgtaa ccattataag ctgcaataaa caagttgatc tcccgatccg
     2461 tcgacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc
     2521 taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa
     2581 tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt
     2641 gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct
     2701 gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc
     2761 cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta
     2821 tgtggcgcgg tattatcccg tattgacgcc gggcaagagc aactcggtcg ccgcatacac
     2881 tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc
     2941 atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac
     3001 ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg
     3061 gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac
     3121 gagcgtgaca ccacgatgcc tgtagcaatg gcaacaacgt tgcgcaaact attaactggc
     3181 gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt
     3241 gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga
     3301 gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc
     3361 cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag
     3421 atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca
     3481 tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc
     3541 ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca
     3601 gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc
     3661 tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta
     3721 ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgttctt
     3781 ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc
     3841 gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg
     3901 ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg
     3961 tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag
     4021 ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc
     4081 agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat
     4141 agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg
     4201 gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc
     4261 tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt
     4321 accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca
     4381 gtgagcgagg aagcggaaga gcgcccaata cgcaaaccgc ctctccccgc gcgttggccg
     4441 attcattaat gcagctggca cgacaggttt cccgactgga aagcgggcag tgagcgcaac
     4501 gcaattaatg tgagttagct cactcattag gcaccccagg ctttacactt tatgcttccg
     4561 gctcgtatgt tgtgtggaat tgtgagcgga taacaatttc acacaggaaa cagctatgac
     4621 catgattacg ccaagctcta gctagaggtc gacggtaaac gctagcaatg tcaaggcctc
     4681 tcactctctg atattcattt ctttgcaagt tataaatact gaataataag atgacatgaa
     4741 ctactactgc tagagatttt ccacactgac taaaagggtc tgagggatct ctagttacca
     4801 gagtcacaca acagacgggc acacactact tgaagcactc aaggcaagct ttattgaggc
     4861 ttaagcagtg ggttccctag ttagccagag agctcccagg ctcagatctg gtctaaccag
     4921 agagacccag tacaggcaaa aagcagctgc ttatatgcag gatctgaggg ctcgccactc
     4981 cccagtcccg cccaggccac gcctccctgg aaagtcccca gcggaaagtc ccttgtagca
     5041 agctcgatat cagcagttct tgaagtactc cggatgcagc tctcgggcca cgtgatgaaa
     5101 tgctaggcgg ctgtcaaacc tccactctaa cacttctctc tccgggtcat ccatcccatg
     5161 caggctcaca gggtgtaaca agctggtgtt ctctccttta ttggcctctt ctaccttatc
     5221 tggctcaact ggtactagct tgtagcacca tccaaaggtc agtggatatc tgacccctgg
     5281 ccctggtgtg tagttctgct aatcagggaa gtagccttgt gtgtggtaga tccacagatc
     5341 aaggatatct tgtcttcgtt gggagtgaat tagcccttcc agtcccctct tttcttttaa
     5401 aaagtggcta agatctacag ctgccttgta agtcattggt cttaaaggta cctgaggtgt
     5461 gactggaaaa cccacctcct cctcctcttg tgcttctagc caggcacaat cagcattggt
     5521 agctgctgta ttgctacttg tgattgctcc atgtttttct aggtctcgac tgcagaatta
     5581 attccaggcg gggaggcggc ccaaagggag atccgactcg tctgagggcg aaggcgaaga
     5641 cgcggaagag gccgcagagc cggcagcagg ccgcgggaag gaaggtccgc tggattgagg
     5701 gccgaaggga cgtagcagaa ggacgtcccg cgcagaatcc aggtggcaac acaggcgagc
     5761 agccatggaa aggacgtcag cttccccgac aacaccacgg aattgtcagt gcccaacagc
     5821 cgagcccctg tccagcagcg ggcaaggcag gcggcgatga gttccgccgt ggcaataggg
     5881 agggggaaag cgaaagtccc ggaaaggagc tgacaggtgg tggcaatgcc ccaaccagtg
     5941 ggggttgcgt cagcaaacac agtgcacacc acgccacgtt gcctgacaac gggccacaac
     6001 tcctcataaa gagacagcaa ccaggattta tacaaggagg agaaaatgaa agccatacgg
     6061 gaagcaatag catgatacaa aggcattaaa gcagcgtatc cacatagcgt aaaaggagca
     6121 acatagttaa gaataccagt caatctttca caaattttgt aatccagagg ttgattgttc
     6181 cagacgcgtt tacgccagaa tgcgttcgca cagccgccag ccggtcactc cgttgatggt
     6241 tactcggaac agcagggagc cgtcggggtt gatcaggcgc tcgtcgataa ttttgttgcc
     6301 gttccacagg gtccctgtta cagtgatctt tttgccgtcg aacacggcga tgccttcata
     6361 cggccgtccg aaatagtcga tcatgttcgg cgtaaccccg tcgattacca gtgtgccata
     6421 gtgcaggatc accttaaagt gatgatcatc cacagggtac accaccttaa aaattttttc
     6481 gatctggccc atttggtcgc cgctcagacc ttcatacggg atgatgacat ggatgtcgat
     6541 cttcagccca ttttcaccgc tcaggacaat cctttggatc ggagttacgg acaccccgag
     6601 attctgaaac aaactggaca cacctccctg ttcaaggact tggtccaggt tgtagccggc
     6661 tgtctgtcgc cagtccccaa cgaaatcttc gagtgtgaag accatggatc caccggtgcc
     6721 acctcgagaa agtcgacctt cttcacccac ccaaatacca tggtagtaac ctcgagaaga
     6781 tccaccaagc ttgattccgg aattcgacgt cttgctcttg ctactcccca tctttggtac
     6841 gggttgtggc aagcttatca tcgtgttttt caaaggaaaa ccacgtcccc gtggttcggg
     6901 gggcctagac gtttttttaa cctcgactaa acacatgtaa agcatgtgca ccgaggcccc
     6961 agatcagatc ccatacaatg gggtaccttc tgggcatcct tcagcccctt gttgaatacg
     7021 cttgaggaga gccatttgac tctttccaca actatccaac tcacaacgtg gcactggggt
     7081 tgtgccgcct ttgcaggtgt atcttataca cgtggctttt ggccgcagag gcacctgtcg
     7141 ccaggtgggg ggttccgctg cctgcaaagg gtcgctacag acgttgtttg tcttcaagaa
     7201 gcttccagag gaactgcttc cttcacgaca ttcaacagac cttgcattcc tttggcgaga
     7261 ggggaaagac ccctaggaat gctcgtcaag aagacagggc caggtttccg ggccctcaca
     7321 ttgccaaaag acggcaatat ggtggaaaat aacatataga caaacgcaca ccggccttat
     7381 tccaagcggc ttcggccagt aacgttaggg gggggggagg gagaggggcg gcggccgctt
     7441 agaagagacc acagtctttt aggttattct ttatgatgac gtccgttaca gcatcgaaca
     7501 caaactgcac attcttcgta tccgtggcgc aagtgaagtg ggtgtaaatt tcctttgtgt
     7561 ccttcctttt attgaggtct tcaaactgac actggatata cgcagccgcc tcttcatatg
     7621 tgtttgagcc tgcatattct ggatagcata tcgtgagggg actctttttg atcttctctt
     7681 cgaagaggtc cttcttgttc aggaaaagga tgatggatgt gtccgtaaac cacttgttgt
     7741 tacatatgct atcgaacagc ttcatgcttt cgtgcatccg gttcatttct tcatcctcag
     7801 caagaaccag gtcatagtca ctcagggcca cacagaagat gatggcagtc acgccttcaa
     7861 agcagtgaat ccacttcttc cgctctgatc tctggcctcc cacgtcaaac attttaaaat
     7921 gaagatcttt gaaagtaaag tgggtttcca caattcccgt cgttttcact ctagttctga
     7981 gaacatcctg ctgggttggg atgtaatttg gttgtgctat tctgtccaag tcattcaggt
     8041 agtacgccgc cgaatcgttc agctggtact cccgggatct gttgaagcag gcttgcacac
     8101 cgctgtcctt ccacagtctc tttatgacgc cggcgagctc cgccttgtat agctcgtcca
     8161 tgccgagagt gatcccggcg gcggtcacga actccagcag gaccatgtga tcgcgcttct
     8221 cgttggggtc tttgctcagg gcggactggt agctcaggta gtggttgtcg ggcagcagca
     8281 cggggccgtc gccgatgggg gtgttctgct ggtagtggtc ggcgagctgc acgctgccgt
     8341 cctcgatgtt gtggcggatc ttgaagttca ccttgatgcc gttcttctgc ttgtcggcca
     8401 tgatatagac gttgtggctg ttgtagttgt actccagctt gtgccccagg atgttgccgt
     8461 cctccttgaa gtcgatgccc ttcagctcga tgcggttcac cagggtgtcg ccctcgaact
     8521 tcacctcggc gcgggtcttg tagttgccgt cgtccttgaa gaagatggtg cgctcctgga
     8581 cgtagccttc gggcatggcg gacttgaaga agtcgtgctg cttcatgtgg tcggggtagc
     8641 gggcgaagca catcaggccg tagccgaagg tggtcacgag ggtgggccag ggcacgggca
     8701 gcttgccggt ggtgcagatg aacttcaggg tcagcttgcc gtaggtggca tcgccctcgc
     8761 cctcgccgga cacgctgaac ttgtggccgt ttacgtcgcc gtccagctcg accaggatgg
     8821 gcaccacccc ggtgaacagc tcctcgccct tgctcaccat cgcggtcata aagccttcct
     8881 ctgcagcccc agcaagcacg aagagttggc gagcatcatc cgcacgagca gcgtctccaa
     8941 agtcgatttt caatctcccc atggctctaa tgatggcaat gatggactgg atggtgttgc
     9001 tgtagaccac tgctttgtac tgcttacact cttcctctga gtagccagcc tcgtggataa
     9061 ttttcatctg cttcacaatt gtgctcttcc cggattcacc agcacccagc agcagcagct
     9121 tgacctcgcg cgctgccttc tctccgtcct cccggaggtt gcggtcgatc atcttgctgc
     9181 gctccacggc cgccttgtcc tcagcgctca gtgtgcagcc catggatccg cggccgctct
     9241 agaactagtc tcgagctgcg ctctcaggca cgacacgact cctccgctgc ccaccgcaga
     9301 ctgaggcagc gctgagtcgc cggcgccgca gcgcagatgg tcgcgcccgt gcccccctat
     9361 ctcgcgcctc gcgtggtgcg gtccggctgg gccggcggcg gcgcggacgc gaccaaggtg
     9421 gccgggaagg ggagtttgcg ggggaccggc gagtgacgtc agcgcgcctt cagtgctgag
     9481 gcggcggtgg cgcgcgccgc caggcggggg cgaaggcact gtccgcggtg ctgaagctgg
     9541 cagtgcgcac gcgcctcgcc gcatcctgtt tcccctcccc ctctctgata ggggatgcgc
     9601 aatttgggga atgggggttg ggtgcttgtc cagtgggtcg gggtcggtcg tcaggtaggc
     9661 acccccaccc cgcctcatcc tggtcctaaa acccacttgc actatcgata aactggatct
     9721 ctgctgtccc tgtaataaac ccg
//
