LOCUS       sequence-411129-       10147 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  For generating targeting inversions. C-terminal split GFP, expressed
            in flight muscles..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10147)
  AUTHORS   Stern  D, Kim E, Behrman E
  TITLE     The Janelia Atalanta plasmids provide a simple and efficient 
            CRISPR/Cas9-mediated homology directed repair platform for 
            Drosophila
  JOURNAL   bioRxiv 2023.06.17.545412
REFERENCE   2  (bases 1 to 10147)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "bioRxiv 
            2023.06.17.545412"
COMMENT     Sequence Label: pJAT27
FEATURES             Location/Qualifiers
     source          1..10147
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             149..940
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MGEAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     rep_origin      1186..1774
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     1675..1694
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     1928..1945
                     /label=L4440
                     /note="L4440 vector, forward primer"
     promoter        1977..1995
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     1977..1994
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     promoter        2072..2492
                     /label=dU6-3 promoter
                     /note="RNA polymerase III promoter for Drosophila U6-3 
                     snRNA (Port et al., 2014)"
     protein_bind    2499..2622
                     /gene="mutant version of attR"
                     /label=attR3
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     promoter        join(2645..2650,2651..2668,2669..2675)
                     /label=lac UV5 promoter
                     /note="E. coli lac promoter with an ""up"" mutation"
     promoter        2718..2822
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(2823..2891,2892..3683)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(3041..3060)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     primer_bind     4204..4223
                     /label=ccdB-fwd
                     /note="ccdB gene, forward primer"
     protein_bind    complement(4363..4486)
                     /gene="mutant version of attR"
                     /label=attR4
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     CDS             6494..6541
                     /codon_start=1
                     /product="11th beta-strand of superfolder GFP"
                     /label=GFP11
                     /note="associates with GFP(1-10), consisting of the first 
                     10 beta-strands, to reconstitute fluorescent GFP (Cabantous
                     et al., 2005; Kamiyama et al., 2016)"
                     /translation="RDHMVLHEYVNAAGIT"
     polyA_signal    6652..6773
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(6689..6708)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     6743..6762
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     protein_bind    6805..6928
                     /gene="mutant version of attR"
                     /label=attR5
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     promoter        join(6951..6956,6957..6974,6975..6981)
                     /label=lac UV5 promoter
                     /note="E. coli lac promoter with an ""up"" mutation"
     CDS             7035..7694
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     complement(7101..7120)
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     promoter        7703..8148
                     /gene="sacR"
                     /label=sacB promoter
                     /note="sacB promoter and control region"
     CDS             join(8149..8235,8236..9570)
                     /codon_start=1
                     /gene="Bacillus subtilis sacB"
                     /product="secreted levansucrase that renders bacterial 
                     growth sensitive to sucrose"
                     /label=SacB
                     /note="negative selection marker"
                     /translation="MNIKKFAKQATVLTFTTALLAGGATQAFAKETNQKPYKETYGISH
                     ITRHDMLQIPEQQKNEKYQVPEFDSSTIKNISSAKGLDVWDSWPLQNADGTVANYHGYH
                     IVFALAGDPKNADDTSIYMFYQKVGETSIDSWKNAGRVFKDSDKFDANDSILKDQTQEW
                     SGSATFTSDGKIRLFYTDFSGKHYGKQTLTTAQVNVSASDSSLNINGVEDYKSIFDGDG
                     KTYQNVQQFIDEGNYSSGDNHTLRDPHYVEDKGHKYLVFEANTGTEDGYQGEESLFNKA
                     YYGKSTSFFRQESQKLLQSDKKRTAELANGALGMIELNDDYTLKKVMKPLIASNTVTDE
                     IERANVFKMNGKWYLFTDSRGSKMTIDGITSNDIYMLGYVSNSLTGPYKPLNKTGLVLK
                     MDLDPNDVTFTYSHFAVPQAKGNNVVITSYMTNRGFYADKQSTFAPSFLLNIKGKKTSV
                     VKDSILEQGQLTVNK"
     protein_bind    complement(9609..9732)
                     /gene="mutant version of attR"
                     /label=attR6
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(9824..9843)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(9825..9843)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
ORIGIN
        1 ggcttgttat gactgttttt ttggggtaca gtctatgcct cgggcatcca agcagcaagc
       61 gcgttacgcc gtgggtcgat gtttgatgtt atggagcagc aacgatgtta cgcagcaggg
      121 cagtcgccct aaaacaaagt taaacatcat gggggaagcg gtgatcgccg aagtatcgac
      181 tcaactatca gaggtagttg gcgtcatcga gcgccatctc gaaccgacgt tgctggccgt
      241 acatttgtac ggctccgcag tggatggcgg cctgaagcca cacagtgata ttgatttgct
      301 ggttacggtg accgtaaggc ttgatgaaac aacgcggcga gctttgatca acgacctttt
      361 ggaaacttcg gcttcccctg gagagagcga gattctccgc gctgtagaag tcaccattgt
      421 tgtgcacgac gacatcattc cgtggcgtta tccagctaag cgcgaactgc aatttggaga
      481 atggcagcgc aatgacattc ttgcaggtat cttcgagcca gccacgatcg acattgatct
      541 ggctatcttg ctgacaaaag caagagaaca tagcgttgcc ttggtaggtc cagcggcgga
      601 ggaactcttt gatccggttc ctgaacagga tctatttgag gcgctaaatg aaaccttaac
      661 gctatggaac tcgccgcccg actgggctgg cgatgagcga aatgtagtgc ttacgttgtc
      721 ccgcatttgg tacagcgcag taaccggcaa aatcgcgccg aaggatgtcg ctgccgactg
      781 ggcaatggag cgcctgccgg cccagtatca gcccgtcata cttgaagcta gacaggctta
      841 tcttggacaa gaagaagatc gcttggcctc gcgcgcagat cagttggaag aatttgtcca
      901 ctacgtgaaa ggcgagatca ccaaggtagt cggcaaataa tgtctagcta gaaattcgtt
      961 caagccgacg ccgcttcgcg gcgcggctta actcaagcgt tagatgcact aagcacataa
     1021 ttgctcacag ccaaactatc aggtcaagtc tgcttttatt atttttaagc gtgcataata
     1081 agccctacac aaattgggag atatatcatg catgaccaaa atcccttaac gtgagttttc
     1141 gttccactga gcgtcagacc ccgtagaaaa gatcaaagga tcttcttgag atcctttttt
     1201 tctgcgcgta atctgctgct tgcaaacaaa aaaaccaccg ctaccagcgg tggtttgttt
     1261 gccggatcaa gagctaccaa ctctttttcc gaaggtaact ggcttcagca gagcgcagat
     1321 accaaatact gtccttctag tgtagccgta gttaggccac cacttcaaga actctgtagc
     1381 accgcctaca tacctcgctc tgctaatcct gttaccagtg gctgctgcca gtggcgataa
     1441 gtcgtgtctt accgggttgg actcaagacg atagttaccg gataaggcgc agcggtcggg
     1501 ctgaacgggg ggttcgtgca cacagcccag cttggagcga acgacctaca ccgaactgag
     1561 atacctacag cgtgagctat gagaaagcgc cacgcttccc gaagggagaa aggcggacag
     1621 gtatccggta agcggcaggg tcggaacagg agagcgcacg agggagcttc cagggggaaa
     1681 cgcctggtat ctttatagtc ctgtcgggtt tcgccacctc tgacttgagc gtcgattttt
     1741 gtgatgctcg tcaggggggc ggagcctatg gaaaaacgcc agcaacgcgg cctttttacg
     1801 gttcctggcc ttttgctggc cttttgctca catgttcttt cctgcgttat cccctgattc
     1861 tgtggataac cgtattaccg cctttgagtg agctgatacc gctcgccgca gccgaacgac
     1921 cgagcgcagc gagtcagtga gcgaggaaat cgtcacctgc aggaaggttt aaacgcattt
     1981 aggtgacact atagaagtgt gtatcgctcg agggatccga attcgaagtc ttggtacgga
     2041 ggggatccga tcgacaagcc gaacatatgg gttttttgct cacctgtgat tgctcctact
     2101 caaatacaaa aacatcaaat tttctgtcaa taaagcatat ttatttatat ttattttaca
     2161 ggaaagaatt ccttttaaag tgtattttaa cctataatga aaaacgatta aaaaaaatac
     2221 ataaaataat tcgaaaattt ttgaatagcc caggttgata aaaattcatt tcatacgttt
     2281 tataacttat gcccctaagt attttttgac catagtgttt caattctaca ttaattttac
     2341 agagtagaat gaaacgccac ctactcagcc aagaggcgaa aaggttagct cgccaagcag
     2401 agagggcgcc agtgctcact actttttata attctcaact tctttttcca gactcagttc
     2461 gtatatatag acctattttc aatttaacgt cggggcctca actttgtata ataaagttga
     2521 acgagaaacg taaaatgata taaatatcaa tatattaaat tagattttgc ataaaaaaca
     2581 gactacataa tactgtaaaa cacaacatat ccagtcacta tgggccgcat taggcacccc
     2641 aggctttaca ctttatgctt ccggctcgta taatgtgtgg attttgagtt aggatccgtc
     2701 gagattttca ggagctacgc ggaaccccta tttgtttatt tttctaaata cattcaaata
     2761 tgtatccgct catgagacaa taaccctgat aaatgcttca ataatattga aaaaggaaga
     2821 gtatgagtat tcaacatttc cgtgtcgccc ttattccctt ttttgcggca ttttgccttc
     2881 ctgtttttgc tcacccagaa acgctggtga aagtaaaaga tgctgaagat cagttgggtg
     2941 cacgagtggg ttacatcgaa ctggatctca acagcggtaa gatccttgag agttttcgcc
     3001 ccgaagaacg ttttccaatg atgagcactt ttaaagttct gctatgtggc gcggtattat
     3061 cccgtattga cgccgggcaa gagcaactcg gtcgccgcat acactattct cagaatgact
     3121 tggttgagta ctcaccagtc acagaaaagc atcttacgga tggcatgaca gtaagagaat
     3181 tatgcagtgc tgccataacc atgagtgata acactgcggc caacttactt ctgacaacga
     3241 tcggaggacc gaaggagcta accgcttttt tgcacaacat gggggatcat gtaactcgcc
     3301 ttgatcgttg ggaaccggag ctgaatgaag ccataccaaa cgacgagcgt gacaccacga
     3361 tgcctgtagc aatggcaaca acgttgcgca aactattaac tggcgaacta cttactctag
     3421 cttcccggca acaattaata gactggatgg aggcggataa agttgcagga ccacttctgc
     3481 gctcggccct tccggctggc tggtttattg ctgataaatc tggagccggt gagcgtgggt
     3541 cccgcggtat cattgcagca ctggggccag atggtaagcc ctcccgtatc gtagttatct
     3601 acacgacggg gagtcaggca actatggatg aacgaaatag acagatcgct gagataggtg
     3661 cctcactgat taagcattgg taatggatcc ggcttactaa aagccagata acagtatgcg
     3721 tatttgcgcg ctgatttttg cggtataaga atatatactg atatgtatac ccgaagtatg
     3781 tcaaaaagag gtatgctatg aagcagcgta ttacagtgac agttgacagc gacagctatc
     3841 agttgctcaa ggcatatatg atgtcaatat ctccggtctg gtaagcacaa ccatgcagaa
     3901 tgaagcccgt cgtctgcgtg ccgaacgctg gaaagcggaa aatcaggaag ggatggctga
     3961 ggtcgcccgg tttattgaaa tgaacggctc ttttgctgac gagaacaggg gctggtgaaa
     4021 tgcagtttaa ggtttacacc tataaaagag agagccgtta tcgtctgttt gtggatgtac
     4081 agagtgatat tatcgataca ccgggccgtc gcatggttat tccacttgcg tctgcgcgct
     4141 tgctctcaga taaagtctcc cgtgaacttt acccggtggt gcatatcggg gatgaaagct
     4201 ggcgcatgat gaccaccgat atggccagtg tgccggtttc cgttatcggg gaagaagtgg
     4261 ctgatctcag ccaccgcgaa aatgacatca aaaacgccat taacctgatg ttctggggaa
     4321 tataatgtca ggctccctta tacacagcca gtctgcacgt cgcatagtga ctggatatgt
     4381 tgtgttttac agtattatgt agtctgtttt ttatgcaaaa tctaatttaa tatattgata
     4441 tttatatcat tttacgtttc tcgttcaact tttctataca aagttgtcca gctggagcga
     4501 gaccggagat actcgaattc gttgtcggct catatacatg ggcgaaataa tttcgaatat
     4561 gttttaaaaa taaccaaaga cattagaaag agatcgccaa tacttataca ttatgtctat
     4621 gtgtgccatg tggtagcatg agccaaaaag cttctcgaaa ttacgaatta catatagacg
     4681 aatatgtatg tgactttagt ttcgaaataa tttcgagaat tttaaaaata acgcattcgt
     4741 taaaagttcg cgtcaattcg aatcgaattt tcgattgtcg atttagtgtg gattgtcgaa
     4801 aatcgttccg ccttcgaagt ttactgaaag gaatcattgc gatctcgtga attgcttgta
     4861 tgagaacacg ccaccatatc gagatacctg ttcaaaatat cgaaatcgcg ttccaaacgg
     4921 tagtagttac cagttgagtg agttgtggtg acagtttaac ctctctacat ttgtaataat
     4981 aagcattgtt ttggcgtatc aatagtctgt agattttttc acaaagaaac ctggagaaat
     5041 gtacacaaat atctaaatga aaattggcgt tacgtaacaa ataccaacgc cccgaaaatc
     5101 cgtatatatg caaagcattc tgatatatat atatataagt ggtatacata tacatattcg
     5161 tatcggatcc atgatctata tttaggaacc ctcgaccgcc ggagtataaa tagaggcgct
     5221 tcgtctacgg agcgacaatt caattcaaac aagcaaagtg aacacgtcgc taagcgaaag
     5281 ctaagcaaat aaacaagcgc agctgaacaa gctaaacaat cggggtaccg ctagagtcga
     5341 cggtaccgcg ggcccgggat ccaccggtcg ccacatgaag atcgaggagg gcaagttggt
     5401 catctggatt aatggagata agggctataa tggactggca gaggttggaa aaaaatttga
     5461 gaaggataca ggcattaagg tcacagttga gcatccagat aagttggagg agaagttccc
     5521 gcaggtggct gcgacgggtg acggacccga catcatattc tgggctcatg accgtttcgg
     5581 aggatacgcc cagtccggct tgttggcgga aattacccct gataaagctt ttcaggacaa
     5641 actctacccc ttcacctggg atgctgtccg atacaacggc aagctcattg cataccccat
     5701 tgcagttgaa gcactgagtc tgatttacaa taaagacctg ttgccgaatc ctccgaagac
     5761 ctgggaggag attcccgctc tggacaagga gctgaaggct aagggcaagt ccgccctgat
     5821 gttcaacctg caagagccgt actttacttg gcccctgatc gcagccgacg gcggatacgc
     5881 cttcaaatac gagaacggaa agtacgatat aaaggatgtc ggagtcgaca acgccggcgc
     5941 taaagcaggc ctgactttcc tggtggacct cataaagaac aagcatatga atgccgacac
     6001 cgattacagt atagcggaag ccgcgttcaa caagggcgag actgccatga caataaacgg
     6061 accctgggca tggtccaaca ttgacacgag caaggtgaac tacggtgtaa cggtgttgcc
     6121 tacgttcaag ggacagccga gtaaaccatt cgttggcgtt ttgtcggctg gaattaatgc
     6181 tgctagtccc aataaggagt tggctaagga gttcttggag aactacttgc tgacagacga
     6241 gggcttggag gcagtaaaca aggataagcc cctgggtgcg gttgccctca agtcctatga
     6301 agaagagctc gtaaaagacc cgcgtattgc tgcgaccatg gagaatgccc agaagggtga
     6361 gattatgccc aatatacccc agatgtccgc gttttggtat gcagttcgca ccgcggttat
     6421 aaacgcagcc tcgggacggc agaccgtgga tgaggacttg aaagacgccc agacacgcat
     6481 aaccaaacag tttcgcgacc atatggttct gcatgaatat gtcaacgcag caggcattac
     6541 ttaaagatca taatcagcca taccacattt gtagaggttt tacttgcttt aaaaaacctc
     6601 ccacacctcc ccctgaacct gaaacataaa atgaatgcaa ttgttgttgt taacttgttt
     6661 attgcagctt ataatggtta caaataaagc aatagcatca caaatttcac aaataaagca
     6721 tttttttcac tgcattctag ttgtggtttg tccaaactca tcaatgtatc ttaaagctta
     6781 tcgatacgcc catggtctca ccatcaactt tgtatacaaa agttgaacga gaaacgtaaa
     6841 atgatataaa tatcaatata ttaaattaga ttttgcataa aaaacagact acataatact
     6901 gtaaaacaca acatatccag tcactatggg ccgcattagg caccccaggc tttacacttt
     6961 atgcttccgg ctcgtataat gtgtggattt tgagttagga tccgtcgaga ttttcaggag
     7021 ctaaggaagc taaaatggag aaaaaaatca ctggatatac caccgttgat atatcccaat
     7081 ggcatcgtaa agaacatttt gaggcatttc agtcagttgc tcaatgtacc tataaccaga
     7141 ccgttcagct ggatattacg gcctttttaa agaccgtaaa gaaaaataag cacaagtttt
     7201 atccggcctt tattcacatt cttgcccgcc tgatgaatgc tcatccggaa ttccgtatgg
     7261 caatgaaaga cggtgagctg gtgatatggg atagtgttca cccttgttac accgttttcc
     7321 atgagcaaac tgaaacgttt tcatcgctct ggagtgaata ccacgacgat ttccggcagt
     7381 ttctacacat atattcgcaa gatgtggcgt gttacggtga aaacctggcc tatttcccta
     7441 aagggtttat tgagaatatg tttttcgtgt cagccaatcc ctgggtgagt ttcaccagtt
     7501 ttgatttaaa cgtggccaat atggacaact tcttcgcccc cgttttcacc atgggcaaat
     7561 attatacgca aggcgacaag gtgctgatgc cgctggcgat tcaggttcat catgccgttt
     7621 gtgatggctt ccatgtcggc agaatgctta atgaattaca acagtactgc gatgagtggc
     7681 agggcggggc gtaaacgcgt ggcacatata cctgccgttc actattattt agtgaaatga
     7741 gatattatga tattttctga attgtgatta aaaaggcaac tttatgccca tgcaacagaa
     7801 actataaaaa atacagagaa tgaaaagaaa cagatagatt ttttagttct ttaggcccgt
     7861 agtctgcaaa tccttttatg attttctatc aaacaaaaga ggaaaataga ccagttgcaa
     7921 tccaaacgag agtctaatag aatgaggtcg aaaagtaaat cgcgcgggtt tgttactgat
     7981 aaagcaggca agacctaaaa tgtgtaaagg gcaaagtgta tactttggcg tcacccctta
     8041 catattttag gtcttttttt attgtgcgta actaacttgc catcttcaaa caggagggct
     8101 ggaagaagca gaccgctaac acagtacata aaaaaggaga catgaacgat gaacatcaaa
     8161 aagtttgcaa aacaagcaac agtattaacc tttactaccg cactgctggc aggaggcgca
     8221 actcaagcgt ttgcgaaaga aacgaaccaa aagccatata aggaaacata cggcatttcc
     8281 catattacac gccatgatat gctgcaaatc cctgaacagc aaaaaaatga aaaatatcaa
     8341 gttcctgaat tcgattcgtc cacaattaaa aatatctctt ctgcaaaagg cctggacgtt
     8401 tgggacagct ggccattaca aaacgctgac ggcactgtcg caaactatca cggctaccac
     8461 atcgtctttg cattagccgg agatcctaaa aatgcggatg acacatcgat ttacatgttc
     8521 tatcaaaaag tcggcgaaac ttctattgac agctggaaaa acgctggccg cgtctttaaa
     8581 gacagcgaca aattcgatgc aaatgattct atcctaaaag accaaacaca agaatggtca
     8641 ggttcagcca catttacatc tgacggaaaa atccgtttat tctacactga tttctccggt
     8701 aaacattacg gcaaacaaac actgacaact gcacaagtta acgtatcagc atcagacagc
     8761 tctttgaaca tcaacggtgt agaggattat aaatcaatct ttgacggtga cggaaaaacg
     8821 tatcaaaatg tacagcagtt catcgatgaa ggcaactaca gctcaggcga caaccatacg
     8881 ctgagagatc ctcactacgt agaagataaa ggccacaaat acttagtatt tgaagcaaac
     8941 actggaactg aagatggcta ccaaggcgaa gaatctttat ttaacaaagc atactatggc
     9001 aaaagcacat cattcttccg tcaagaaagt caaaaacttc tgcaaagcga taaaaaacgc
     9061 acggctgagt tagcaaacgg cgctctcggt atgattgagc taaacgatga ttacacactg
     9121 aaaaaagtga tgaaaccgct gattgcatct aacacagtaa cagatgaaat tgaacgcgcg
     9181 aacgtcttta aaatgaacgg caaatggtac ctgttcactg actcccgcgg atcaaaaatg
     9241 acgattgacg gcattacgtc taacgatatt tacatgcttg gttatgtttc taattcttta
     9301 actggcccat acaagccgct gaacaaaact ggccttgtgt taaaaatgga tcttgatcct
     9361 aacgatgtaa cctttactta ctcacacttc gctgtacctc aagcgaaagg aaacaatgtc
     9421 gtgattacaa gctatatgac aaacagagga ttctacgcag acaaacaatc aacgtttgcg
     9481 ccaagcttcc tgctgaacat caaaggcaag aaaacatctg ttgtcaaaga cagcatcctt
     9541 gaacaaggac aattaacagt taacaaataa atgtcaggct cccttataca cagccagtct
     9601 gcacgtcgca tagtgactgg atatgttgtg ttttacagta ttatgtagtc tgttttttat
     9661 gcaaaatcta atttaatata ttgatattta tatcatttta cgtttctcgt tcaacttttt
     9721 aatacaaagt tgcctccgga acataatggt gcatctagat gcccattcct gtagacttct
     9781 taattaagac gtcagaattc tcgaggcggc cgcatgtgag tctccctata gtgagtcgta
     9841 ttaatttcgc gggcgcgtca gcgggagagc cgttgtaagg cggcagactt tgctcatgtt
     9901 accgatgcta ttcggaagaa cggcaactaa gctgccgggt ttgaaacacg gatgatctcg
     9961 cggagggtag catgttgatt gtaacgatga cagagcgttg ctgcctgtga tcaattcggg
    10021 cacgaaccca gtggacataa gcctgttcgg ttcgtaagct gtaatgcaag tagcgtatgc
    10081 gctcacgcaa ctggtccaga accttgaccg aacgcagcgg tggtaacggc gcagtggcgg
    10141 ttttcat
//
